CmaCh12G000050 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh12G000050 vs. Swiss-Prot
Match: PGL3_ARATH (Polygalacturonase 1 beta-like protein 3 OS=Arabidopsis thaliana GN=PGL3 PE=2 SV=2)

HSP 1 Score: 783.9 bits (2023), Expect = 1.3e-225
Identity = 385/622 (61.90%), Postives = 475/622 (76.37%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGD---------EDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSKA 71
           L L  F    FNV   G          E+NPFTP+A L RYWNKE+R   P+  FL SKA
Sbjct: 6   LLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKA 65

Query: 72  SPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNF 131
           SPL AV SA F+KLAA N+L +R P FCSAANL CFPDL  SL KH+ D  F+VY  +NF
Sbjct: 66  SPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNF 125

Query: 132 TTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYA 191
           T YG+ RAGG DSFKNYS D N++ DSFRRYS  A  H D+F+ YG ++NV ++GFN+Y 
Sbjct: 126 TNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYG 185

Query: 192 SKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRG 251
           +   GG G FT Y+ + N P  RFT+YS    GRSQ FK+Y+  ANAG+ QSF +YGK G
Sbjct: 186 TFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNG 245

Query: 252 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAI 311
           NG PNEF++YG ++NVIGSGFS+YG  GN ANDTFT+YG++ N PQNNF +YG  GN A+
Sbjct: 246 NGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAV 305

Query: 312 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 371
           ++F++YRD+ANVGDDSF SY+K+SNS KVNF NYG+S N GS+ F+GYG+      + FK
Sbjct: 306 DTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFK 365

Query: 372 IYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMA 431
            Y  N+TFKDYAK+G++FA+Y NVS+  A    +G    +W+EPGKFFRES L++GTV+ 
Sbjct: 366 TYTPNSTFKDYAKKGVAFAKY-NVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIP 425

Query: 432 MPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPS 491
           MPDIKDKMPKRSFLPRSI++KLPFS+SKLG +K+IFHA +NS+M  ++T+ + ECER PS
Sbjct: 426 MPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPS 485

Query: 492 AGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSC 551
            GETKRCVGS EDM+DFATSVLGR+VV+R TEN  GSK  ++IG V  + GGK+TK+VSC
Sbjct: 486 VGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSC 545

Query: 552 HQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGS 611
           HQSL+PYLLYYCHSVPKVRVYEA+LL+  S   INHG+AICH+DTS+W PSHGAF+ALGS
Sbjct: 546 HQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGS 605

Query: 612 APGRVEVCHWIFENDMTWTVAD 624
            PGR+EVCHWIFENDM W +AD
Sbjct: 606 KPGRIEVCHWIFENDMNWAIAD 626

BLAST of CmaCh12G000050 vs. Swiss-Prot
Match: PGL1_ARATH (Polygalacturonase 1 beta-like protein 1 OS=Arabidopsis thaliana GN=PGL1 PE=2 SV=2)

HSP 1 Score: 763.1 bits (1969), Expect = 2.4e-219
Identity = 377/623 (60.51%), Postives = 466/623 (74.80%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGDED----------NPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 71
           ++L  FL   ++V  AG E           NPFTP+A L RYWNK++ +  P   FL SK
Sbjct: 6   VFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSK 65

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 131
           ASPLTAV SA FAKLA++N L  RLP FCSAANL CFPDL P+L KH+ D  F+VY  RN
Sbjct: 66  ASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRN 125

Query: 132 FTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 191
           FT YG+   GG DSFK YS + N + DSFRRYS  A  H D+F+ Y  ++NV ++ FN+Y
Sbjct: 126 FTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSY 185

Query: 192 ASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKR 251
            + A GG+G F  Y+   N P  RF +YS +  GR+Q FK+Y+  AN+G  QSF +YGK 
Sbjct: 186 GTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKN 245

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 311
           GNG PNEFS+YG ++NVIGS FS+YG +GN ANDTF  YG + N PQNNF+SYG  GN A
Sbjct: 246 GNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAA 305

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N GS+ FSGYG+      + F
Sbjct: 306 VDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDF 365

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K Y  N+TFKDY K G+ FA+Y+  S GG K +VN     +WVEPGKFFRESML++GT++
Sbjct: 366 KTYTQNSTFKDYTKTGVEFAKYNRSSLGGGK-TVN-----KWVEPGKFFRESMLKEGTLI 425

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKRSFLPRSI+SKLPFS+SK+  +K++FHA DNS+ME ++T+ +RECER P
Sbjct: 426 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 485

Query: 492 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           +  ETKRCVGS EDM+DFATSVLGR+VV+R TE+  GSK  +MIG V  + GG+VTKSVS
Sbjct: 486 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 545

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVPKVRVYE++LLDP+S A INHG+AICH+DTSAW  +HGAF+ LG
Sbjct: 546 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 605

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDM W +AD
Sbjct: 606 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of CmaCh12G000050 vs. Swiss-Prot
Match: GP1_SOLLC (Polygalacturonase-1 non-catalytic subunit beta OS=Solanum lycopersicum GN=GP1 PE=1 SV=1)

HSP 1 Score: 755.0 bits (1948), Expect = 6.6e-217
Identity = 381/620 (61.45%), Postives = 464/620 (74.84%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGDED--NPFTPRAFLSRYWNKEVRNDLPKPTFLFSKASPLTAVQ 71
           L L +F L +FNV   GD +  NPFTP+ +L RYW K++ NDLPKP FL +KASPL A Q
Sbjct: 12  LLLLLFSLPSFNVVVGGDGESGNPFTPKGYLIRYWKKQISNDLPKPWFLLNKASPLNAAQ 71

Query: 72  SAAFAKLAA-QNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGSK 131
            A + KL A QN L+++L  FCS+ANL+C PDL+PSL KH+ D +FA YS++NFT YG+ 
Sbjct: 72  YATYTKLVADQNALTTQLHTFCSSANLMCAPDLSPSLEKHSGDIHFATYSDKNFTNYGTN 131

Query: 132 RAG-GIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAIG 191
             G G+++FKNYS  ENI V+SFRRY  G+    ++F  Y  D NV DQ FN+Y++   G
Sbjct: 132 EPGIGVNTFKNYSEGENIPVNSFRRYGRGS-PRDNKFDNYASDGNVIDQSFNSYSTSTAG 191

Query: 192 GNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPNE 251
           G+GKFT Y  + N PNL FTSYS   TG  Q F  YS+ ANAGDQ F +YGK GNG   E
Sbjct: 192 GSGKFTNYAANANDPNLHFTSYSDQGTGGVQKFTIYSQEANAGDQYFKSYGKNGNGANGE 251

Query: 252 FSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAIESFSSY 311
           F +YG + NVIGS F++YG   NG +  FT+YG   N P+N+F +YG GGNG  E+F+SY
Sbjct: 252 FVSYGNDTNVIGSTFTNYGQTANGGDQKFTSYGFNGNVPENHFTNYGAGGNGPSETFNSY 311

Query: 312 RDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVNT 371
           RDQ+NVGDD+F +Y K++N  + NF NYG+S N G+D F+ YG+ GN   + FK YGVN 
Sbjct: 312 RDQSNVGDDTFTTYVKDANGGEANFTNYGQSFNEGTDVFTTYGKGGNDPHINFKTYGVNN 371

Query: 372 TFKDYAKRGISFAQYSNVSSG--GAKMSVNGS--LAQRWVEPGKFFRESMLRKGTVMAMP 431
           TFKDY K   +F+ Y N +S    + M VNG   +  RWVEPGKFFRE ML+ GT+M MP
Sbjct: 372 TFKDYVKDTATFSNYHNKTSQVLASLMEVNGGKKVNNRWVEPGKFFREKMLKSGTIMPMP 431

Query: 432 DIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAG 491
           DIKDKMPKRSFLPR I SKLPFS+SK+  +KKIFHA D S +E M+ + L ECERAPSAG
Sbjct: 432 DIKDKMPKRSFLPRVIASKLPFSTSKIAELKKIFHAGDESQVEKMIGDALSECERAPSAG 491

Query: 492 ETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSCHQ 551
           ETKRCV S EDM+DFATSVLGRNVVVR TE+TKGS GNIMIG+VK + GGKVTKSVSCHQ
Sbjct: 492 ETKRCVNSAEDMIDFATSVLGRNVVVRTTEDTKGSNGNIMIGSVKGINGGKVTKSVSCHQ 551

Query: 552 SLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGSAP 611
           +L+PYLLYYCHSVPKVRVYEA++LDP S   INHGVAICH+DTS+W PSHGAF+ALGS P
Sbjct: 552 TLYPYLLYYCHSVPKVRVYEADILDPNSKVKINHGVAICHVDTSSWGPSHGAFVALGSGP 611

Query: 612 GRVEVCHWIFENDMTWTVAD 624
           G++EVCHWIFENDMTW +AD
Sbjct: 612 GKIEVCHWIFENDMTWAIAD 630

BLAST of CmaCh12G000050 vs. Swiss-Prot
Match: PGL2_ARATH (Polygalacturonase 1 beta-like protein 2 OS=Arabidopsis thaliana GN=PGL2 PE=2 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 4.4e-213
Identity = 373/623 (59.87%), Postives = 450/623 (72.23%), Query Frame = 1

Query: 12  LYLSIF-LLSAFNVPAAGDE---------DNPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 71
           L+L  F + S+ NV  AG +         +NPFTP+A L RYWN  +  D PKP+F  SK
Sbjct: 8   LFLCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSK 67

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAP-SLAKHNKDSNFAVYSNR 131
           ASPLTAV S  FA LA+ + L++    FCSAA L CFP+LA  SL KH  D NFA YS +
Sbjct: 68  ASPLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGK 127

Query: 132 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 191
           NFT YGS R  G DSFKNYS  +NI VDSFRRYS  +  H D F+ Y  + NV DQ F T
Sbjct: 128 NFTNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTT 187

Query: 192 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 251
           YA+   GG+G+FT Y    N PN RFTSYS  A GRSQ F +YSEN N G QSF +Y K 
Sbjct: 188 YATGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKN 247

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 311
           GNG PNEFS YGT +NV+ +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGNGA
Sbjct: 248 GNGAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGA 307

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +  F +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKS N+GSD F+GYGQ+    +V F
Sbjct: 308 VYGFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSF 367

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K YG   +FK Y K G+ FA+YSN       +S NG    +WVE GKFFRE+ML++GT+M
Sbjct: 368 KTYGQGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLM 427

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKR+FLPR+I+  LPFSSS +G + ++F A +NSSM  +++  + ECER  
Sbjct: 428 QMPDIKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPA 487

Query: 492 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           S GETKRCVGS EDM+DFATSVLGR VVVR TEN  GSK  ++IG V  + GG VT++VS
Sbjct: 488 SHGETKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVS 547

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVP+VRVYE +LLDP+S   INHGVAICH+DTSAWSPSHGAF+ALG
Sbjct: 548 CHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALG 607

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDMTW + D
Sbjct: 608 SGPGQIEVCHWIFENDMTWNIID 624

BLAST of CmaCh12G000050 vs. Swiss-Prot
Match: GP2_SOLLC (Polygalacturonase non-catalytic subunit AroGP2 OS=Solanum lycopersicum GN=GP2 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 9.2e-211
Identity = 371/621 (59.74%), Postives = 460/621 (74.07%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGDED---NPFTPRAFLSRYWNKEVRNDLPKPTFLFSKASPLTAV 71
           L + +F LS+FN+  A D D   NPFTP+ ++ RYWNK V NDLPKP FL +KASPL A 
Sbjct: 12  LLVLLFSLSSFNIVVAKDGDESGNPFTPKGYVIRYWNKHVSNDLPKPWFLLNKASPLNAA 71

Query: 72  QSAAFAKLAA-QNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGS 131
           Q A + KL A QN LS+ L  FCS+ANL+C PDL PSL KH  D +F  Y N+NFT YG+
Sbjct: 72  QYATYTKLVADQNALSTHLQSFCSSANLMCAPDLLPSLEKHTGDIHFTTYGNKNFTNYGT 131

Query: 132 KRAG-GIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAI 191
              G G+++FKNYS D +  V+SFRRY  G+    ++F  Y  D NV DQ FN+Y++   
Sbjct: 132 NEPGIGVNTFKNYSEDAS--VNSFRRYGRGS-PRDNKFDNYAPDGNVIDQSFNSYSTNTP 191

Query: 192 GGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPN 251
           GG+G+FT Y  +TNVP+LRFT+YS   TG  Q FK+Y E  N+G QSF +YGK GNG  +
Sbjct: 192 GGSGQFTNYAPNTNVPDLRFTAYSDQGTGGEQEFKTYLEQGNSGGQSFKSYGKNGNGADS 251

Query: 252 EFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAIESFSS 311
           +F++YG   NV  S F +YG + NG N  FT+Y    N+PQNNF++YG GGNG  E+F++
Sbjct: 252 KFTSYGNETNVAASTFKNYGQNANGENQNFTSYSTNGNNPQNNFKNYGVGGNGPSETFTN 311

Query: 312 YRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVN 371
           YRD++NVGDD F +Y K++N+ + NF NYG+S N G+D F  YG+ GN   + FK YGVN
Sbjct: 312 YRDESNVGDDKFSNYVKDANAGEANFTNYGQSFNEGTDVFITYGKGGNDPHINFKTYGVN 371

Query: 372 TTFKDYAKRGISFAQYSNVSSGG-AKMS-VNGS--LAQRWVEPGKFFRESMLRKGTVMAM 431
            TFKDY K   +F+ Y N +S   A +S VNG   +  RW+EPGKFFRE ML+ GT+M M
Sbjct: 372 NTFKDYVKDTATFSNYHNKTSQDLASLSEVNGGKKVNNRWIEPGKFFREKMLKSGTIMPM 431

Query: 432 PDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSA 491
           PDIKDKMPKRSFLPR+I +KLPFS+SK+  +KKIFHA ++S +  M+ + L ECERAPS 
Sbjct: 432 PDIKDKMPKRSFLPRAIAAKLPFSTSKIDELKKIFHAANDSQVAKMIGDALSECERAPSP 491

Query: 492 GETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSCH 551
           GETK+CV S EDM+DFATSVLGRNVVVR TENT GSKGNIMIG++K + GGKVTKSVSCH
Sbjct: 492 GETKQCVNSAEDMIDFATSVLGRNVVVRTTENTNGSKGNIMIGSIKGINGGKVTKSVSCH 551

Query: 552 QSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGSA 611
           Q+L+P LLYYCHSVPKVRVYEA++LDP S A INHGVAICH+DTS+W P HGAFIALGS 
Sbjct: 552 QTLYPSLLYYCHSVPKVRVYEADILDPNSKAKINHGVAICHVDTSSWGPRHGAFIALGSG 611

Query: 612 PGRVEVCHWIFENDMTWTVAD 624
           PG++EVCHWIFENDMTW  AD
Sbjct: 612 PGKIEVCHWIFENDMTWATAD 629

BLAST of CmaCh12G000050 vs. TrEMBL
Match: A0A0A0LK44_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G348230 PE=4 SV=1)

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 541/623 (86.84%), Postives = 586/623 (94.06%), Query Frame = 1

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFS 60
           MHRLP   +VPLYL IF LS+ NV  AGDED+PFTP+AFL+RYWNKEVRND PKP+FLFS
Sbjct: 1   MHRLPTLYVVPLYLFIFSLSSLNVSTAGDEDDPFTPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAA+N LSSRLP FCSAANLLCFPDL+PSLAKH++DSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N++VDSFRRYSH AL H DRFS YG DTNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGG+GKFTTYEQS NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300
           GNGGPNEF+ YGT+ANVIGS FSSYG++GNGANDTFTNYGN+QN+PQNNFRSYGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K YGVNTTFKDYAK+GI+FA+YSNVSSGGAKMSV+GSLA+RWVEPGKFFRESMLRKG+VM
Sbjct: 361 KTYGVNTTFKDYAKQGINFAKYSNVSSGGAKMSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKLGVMK+IFHA DNSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERLP 480

Query: 481 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDM+DFATSVLGRNVVV+ TENTKGSKGNIMIG VK + GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE +LLDP + A +NHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of CmaCh12G000050 vs. TrEMBL
Match: A0A061E744_THECC (Polygalacturonase 2 OS=Theobroma cacao GN=TCM_010347 PE=4 SV=1)

HSP 1 Score: 852.0 bits (2200), Expect = 4.4e-244
Identity = 417/614 (67.92%), Postives = 495/614 (80.62%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSKASPLTAVQSA 71
           L  S F +    V A+   +NPFTP+A L RYWNKE+RN +PK  FL SK SPLTAV SA
Sbjct: 68  LSFSSFSVIFGGVGASSVSENPFTPKASLIRYWNKEIRNQIPKTHFLLSKVSPLTAVDSA 127

Query: 72  AFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGSKRAG 131
           +F+KLAAQN L+SRLP FCS+A L CFPDL+PSL KH KD+NFA Y+N+NFT YG+ R  
Sbjct: 128 SFSKLAAQNDLASRLPSFCSSAKLFCFPDLSPSLEKHPKDANFAFYANKNFTNYGTDRLA 187

Query: 132 GIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAIGGNGK 191
           G+DSFKNYS  +N++VDSFRRYS  +  H D FS Y  ++NV DQ FNTY + A GG G 
Sbjct: 188 GVDSFKNYSEGDNVIVDSFRRYSRDSAGHKDLFSNYASESNVVDQSFNTYGAAATGGGGN 247

Query: 192 FTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRGNGGPNEFSA 251
           F  Y +  N PNLRFTSYS D+ GR Q F  Y+ENANAG+ QSF++YGK GNG PN+FS+
Sbjct: 248 FNNYNREVNNPNLRFTSYSDDSNGRGQTFTRYTENANAGNGQSFSSYGKNGNGVPNDFSS 307

Query: 252 YGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAIESFSSYRDQ 311
           YG  ANV+GSGFS YG   NGANDTFT+YG + N P N F+SYG+GGN A++SFSSYRDQ
Sbjct: 308 YGKGANVVGSGFSGYGESANGANDTFTSYGFDSNVPLNTFKSYGDGGNAAVDSFSSYRDQ 367

Query: 312 ANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVNTTFK 371
           +NVGDDSFQSY+KNSN+AKV+F NYG+S N G+DKF+GYGQ    QS+GFKIYG NTTFK
Sbjct: 368 SNVGDDSFQSYAKNSNAAKVDFNNYGQSFNEGTDKFTGYGQGAEGQSIGFKIYGRNTTFK 427

Query: 372 DYAKRGISFAQYSNVSSGG-AKMSVNGSLAQRWVEPGKFFRESMLRKGTVMAMPDIKDKM 431
           DY K+G+SF +Y+N SS   A++  + S   +WVEPGKFFRE ML++GTV+ MPDI+DKM
Sbjct: 428 DYTKKGVSFGRYNNESSAETAQVKASVSAVNKWVEPGKFFREKMLKRGTVLPMPDIRDKM 487

Query: 432 PKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAGETKRCV 491
           P+RSFLPR+I+SKLPFSSSK+G +K+IFHA DNS++ET+M + L+ECERAPSAGETKRCV
Sbjct: 488 PERSFLPRTIVSKLPFSSSKIGELKRIFHAGDNSTLETVMLDALKECERAPSAGETKRCV 547

Query: 492 GSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSCHQSLFPYL 551
           GS EDM+DFATSVLGRNV VR TEN KGSK NI IG V+ +  GKVTKSVSCHQSL+PYL
Sbjct: 548 GSAEDMIDFATSVLGRNVEVRTTENVKGSKQNIKIGTVRGINSGKVTKSVSCHQSLYPYL 607

Query: 552 LYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGSAPGRVEVC 611
           LYYCHSVPKVRVYEA++LDP S   INHGVAICHLDTSAWS  HGAF+ALGS PGR+EVC
Sbjct: 608 LYYCHSVPKVRVYEADILDPNSREKINHGVAICHLDTSAWSSGHGAFLALGSGPGRIEVC 667

Query: 612 HWIFENDMTWTVAD 624
           HWIFENDMTWT+AD
Sbjct: 668 HWIFENDMTWTIAD 681

BLAST of CmaCh12G000050 vs. TrEMBL
Match: W9RD10_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_027781 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 1.3e-240
Identity = 412/601 (68.55%), Postives = 491/601 (81.70%), Query Frame = 1

Query: 27  AGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSKASPLTAVQSAAFAKLAAQNTLSSRL 86
           AG E+NPF+P+A L RYWNKE+RND+ KP+FL SKASPLTAVQ+A FAKLAAQN+LS+RL
Sbjct: 8   AGGEENPFSPKAHLIRYWNKEIRNDIEKPSFLLSKASPLTAVQTAVFAKLAAQNSLSTRL 67

Query: 87  PQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNFTTYGSKRAGGIDSFKNYSNDENIL 146
           P+FCSAA+LLCFPDL PSL  H+KDS+F+ Y +RNFT YG+ R GG D FKNYS + NI 
Sbjct: 68  PEFCSAAHLLCFPDLGPSLESHDKDSSFSGYVDRNFTNYGTDRIGGADVFKNYSPEVNIP 127

Query: 147 VDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYASKAIGGNGKFTTYEQSTNVPNLRF 206
           V+SFR YS  +  H D+F  Y  + NV DQ FNTYA+K  GG G F+ Y +S NVPNLRF
Sbjct: 128 VNSFRSYSRHSTGHDDKFHNYAFEGNVVDQSFNTYATKTTGGAGDFSKYAESVNVPNLRF 187

Query: 207 TSYSTDATGRSQVFKSYSENANAGDQSFANYGKRGNGGPNEFSAYGTNANVIGSGFSSYG 266
           TSYS    GR Q FK YS NAN+GDQSF+NYGK GNG  N+FS+YG ++NVIGS FSSY 
Sbjct: 188 TSYSFQTNGREQNFKGYSVNANSGDQSFSNYGKDGNGAENDFSSYGNSSNVIGSEFSSYQ 247

Query: 267 SDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAIESFSSYRDQANVGDDSFQSYSKNSN 326
              NG  DTFT Y  + N+PQNNFR+YG+G N A+E F+SYRDQ+NVGDDSFQSY+KNSN
Sbjct: 248 RTSNGGQDTFTAYAFDGNNPQNNFRNYGDGANAAVEKFTSYRDQSNVGDDSFQSYAKNSN 307

Query: 327 SAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFKIYGVNTTFKDYAKRGISFAQYSNVS 386
             ++ F+NYGKS N G+DKF+GYG++    S+GFKIYGVN TFK+YA+ G++F++Y+NVS
Sbjct: 308 GEEIKFSNYGKSFNEGTDKFTGYGKDATGHSIGFKIYGVNNTFKEYAEDGVTFSRYTNVS 367

Query: 387 SG----GAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMAMPDIKDKMPKRSFLPRSILSK 446
           SG      K++ +GSL +RWVEPGKFFRES L+KG VM MPDIKDKMP+RSFLPR I SK
Sbjct: 368 SGPEESANKLAFSGSLVKRWVEPGKFFRESKLKKGVVMPMPDIKDKMPERSFLPRVITSK 427

Query: 447 LPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAGETKRCVGSVEDMLDFATSV 506
           LPFS+SK   +KKIFHA DNS+ME ++ +TL ECERAPS GETKRCV SVEDM+DFATSV
Sbjct: 428 LPFSTSKASDLKKIFHAADNSTMEKIIVDTLAECERAPSRGETKRCVSSVEDMIDFATSV 487

Query: 507 LGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSCHQSLFPYLLYYCHSVPKVRVY 566
           LGRNVV R TE+  GSK NIMIG+VK + GGKVT+SVSCHQSLFPYL+YYCHSVPKVRVY
Sbjct: 488 LGRNVVARTTESVSGSKKNIMIGSVKGINGGKVTQSVSCHQSLFPYLVYYCHSVPKVRVY 547

Query: 567 EAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGSAPGRVEVCHWIFENDMTWTVA 624
            A++LDP S A INHGVAICHLDTSAWSP+HGAF+ALGS PGR+EVCHWIFEND+TW+V+
Sbjct: 548 TADILDPNSKAKINHGVAICHLDTSAWSPTHGAFLALGSKPGRIEVCHWIFENDLTWSVS 607

BLAST of CmaCh12G000050 vs. TrEMBL
Match: A0A067GRT8_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g036311mg PE=4 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 3.9e-240
Identity = 419/631 (66.40%), Postives = 495/631 (78.45%), Query Frame = 1

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGD-------EDNPFTPRAFLSRYWNKEVRNDLP 60
           +H L +PL   L LS+  L+  N     +       + NPFTP+A L RYW+KE+R++LP
Sbjct: 2   LHLLSSPLFFLLLLSLCFLNHVNYLGGTEISDSIDMKQNPFTPKAHLIRYWDKEIRSNLP 61

Query: 61  KPTFLFSKASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSN 120
           K  FL SKASPL AV SA+FAKLAAQN LSS LP FCS+A L CFPDL+ SL KH+ DSN
Sbjct: 62  KSPFLLSKASPLNAVDSASFAKLAAQNALSSVLPSFCSSAKLFCFPDLSNSLEKHDTDSN 121

Query: 121 FAVYSNRNFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNV 180
           FA Y N+NFT YG+ R GG DSFKNYS+ +N+ VDSFRRYS  A+ H D+FS Y   +NV
Sbjct: 122 FARYLNKNFTNYGTSRVGGADSFKNYSDGDNLPVDSFRRYSRDAVGHQDKFSNYAPGSNV 181

Query: 181 PDQGFNTYASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQS 240
            DQ FNTY + A GG G F  Y +  NVP+LRFTSY+++  GR+  F +Y+E  N+G + 
Sbjct: 182 ADQSFNTYGASASGGAGSFKNYNEEVNVPHLRFTSYASEGNGRAHSFTAYAEKTNSGSEE 241

Query: 241 FANYGKRGNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSY 300
           FANYGK  NG PN F+ YG   NVIGSGFS+YG   NGAND+FT+YG + N P N F +Y
Sbjct: 242 FANYGKNANGAPNAFTGYGKETNVIGSGFSNYGEGANGANDSFTSYGEDGNVPNNRFNNY 301

Query: 301 GEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNG 360
           G GGN A+ESFS+YRDQANVGDDSFQSY K+S S KVNFANYG S N G+D F GYG+  
Sbjct: 302 GAGGNAAVESFSNYRDQANVGDDSFQSYGKDSKSGKVNFANYGNSFNEGTDVFKGYGEAA 361

Query: 361 NAQSVGFKIYGVNTTFKDYAKRGISFAQYSNVSSGGAKM-SVNGSLAQRWVEPGKFFRES 420
             Q+VGFK+YGVN TFKDYAK G++FA+Y+N SSG   +   +GSL  RWVEPGKFFRES
Sbjct: 362 RGQTVGFKMYGVNNTFKDYAKNGVTFAKYNNDSSGVQNIKKPSGSLLNRWVEPGKFFRES 421

Query: 421 MLRKGTVMAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTET 480
           +L+KG+VMAMPDI+DKMPKRSFLPR+I+SKLPFSSSK+  MK+IFHA DNSSME+++ E 
Sbjct: 422 VLKKGSVMAMPDIQDKMPKRSFLPRTIVSKLPFSSSKIKDMKEIFHAVDNSSMESIIKEA 481

Query: 481 LRECERAPSAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEG 540
           L +CERAPSAGETKRCVGSVEDM+DFATS+LGRNVVVR TEN  GSK  IMIG+VK + G
Sbjct: 482 LGDCERAPSAGETKRCVGSVEDMIDFATSILGRNVVVRTTENVNGSKKKIMIGSVKGING 541

Query: 541 GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPS 600
           GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEA++LD  + A INHGVAICHLDTSAWSP+
Sbjct: 542 GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEADVLDANTKAKINHGVAICHLDTSAWSPT 601

Query: 601 HGAFIALGSAPGRVEVCHWIFENDMTWTVAD 624
           HGAF+ALGS PG++EVCHWIFENDMTWT AD
Sbjct: 602 HGAFLALGSGPGKIEVCHWIFENDMTWTTAD 632

BLAST of CmaCh12G000050 vs. TrEMBL
Match: V4VGJ6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10030947mg PE=4 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 1.1e-239
Identity = 418/631 (66.24%), Postives = 495/631 (78.45%), Query Frame = 1

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGD-------EDNPFTPRAFLSRYWNKEVRNDLP 60
           +H L +PL   L LS+  L+  N     +       + NPFTP+A L RYW+KE+R++LP
Sbjct: 2   LHLLSSPLFFLLLLSLCFLNHVNYLGGTEISDSIDMKQNPFTPKAHLIRYWDKEIRSNLP 61

Query: 61  KPTFLFSKASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSN 120
           K  FL SKASPL AV SA+FAKLAAQN LSS LP FCS+A L CFPDL+ SL KH+ DSN
Sbjct: 62  KSPFLLSKASPLNAVDSASFAKLAAQNALSSVLPSFCSSAKLFCFPDLSNSLEKHDTDSN 121

Query: 121 FAVYSNRNFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNV 180
           FA Y N+NFT YG+ R GG DSFKNYS+ +N+ VDSFRRYS  A+ H D+FS Y   +NV
Sbjct: 122 FARYLNKNFTNYGTSRVGGADSFKNYSDGDNLPVDSFRRYSRDAVGHHDKFSNYAPGSNV 181

Query: 181 PDQGFNTYASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQS 240
            DQ FNTY + A GG G F  Y +  NVP+LRFTSY+++  GR+  F +Y+E  N+G + 
Sbjct: 182 ADQSFNTYGASASGGAGSFKNYNEEVNVPHLRFTSYASEGNGRAHSFTTYAEKTNSGSEE 241

Query: 241 FANYGKRGNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSY 300
           FANYGK  NG PN F+ YG   NVIGSGFS+YG   NGAND+FT+YG + N P N F +Y
Sbjct: 242 FANYGKNANGAPNAFTGYGKETNVIGSGFSNYGEGANGANDSFTSYGEDGNVPNNRFNNY 301

Query: 301 GEGGNGAIESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNG 360
           G GGN A+ESFS+YRDQANVGDDSFQSY K+S S KVNFANYG S N G+D F GYG+  
Sbjct: 302 GAGGNAAVESFSNYRDQANVGDDSFQSYGKDSKSGKVNFANYGNSFNEGTDVFKGYGEAA 361

Query: 361 NAQSVGFKIYGVNTTFKDYAKRGISFAQYSNVSSGGAKM-SVNGSLAQRWVEPGKFFRES 420
             Q+VGFK+YGVN TFKDYAK G++FA+Y+N SSG   +   +GSL  RWVEPGKFFRES
Sbjct: 362 RGQTVGFKMYGVNNTFKDYAKNGVTFAKYNNDSSGVQNIKKPSGSLLNRWVEPGKFFRES 421

Query: 421 MLRKGTVMAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTET 480
           +L+KG+VMAMPDI+DKMPKRSFLPR+I+SKLPFSSSK+  MK+IFHA DNSSME+++ E 
Sbjct: 422 VLKKGSVMAMPDIQDKMPKRSFLPRTIVSKLPFSSSKIKDMKEIFHAVDNSSMESIIKEA 481

Query: 481 LRECERAPSAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEG 540
           L +CERAPSAGETKRCVGSVEDM+DFATS+LGRNVVVR TEN  GSK  I+IG+VK + G
Sbjct: 482 LGDCERAPSAGETKRCVGSVEDMIDFATSILGRNVVVRTTENVNGSKKKIVIGSVKGING 541

Query: 541 GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPS 600
           GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEA++LD  + A INHGVAICHLDTSAWSP+
Sbjct: 542 GKVTKSVSCHQSLFPYLLYYCHSVPKVRVYEADILDANTKAKINHGVAICHLDTSAWSPT 601

Query: 601 HGAFIALGSAPGRVEVCHWIFENDMTWTVAD 624
           HGAF+ALGS PG++EVCHWIFENDMTWT AD
Sbjct: 602 HGAFLALGSGPGKIEVCHWIFENDMTWTTAD 632

BLAST of CmaCh12G000050 vs. TAIR10
Match: AT1G70370.1 (AT1G70370.1 polygalacturonase 2)

HSP 1 Score: 783.9 bits (2023), Expect = 7.5e-227
Identity = 385/622 (61.90%), Postives = 475/622 (76.37%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGD---------EDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSKA 71
           L L  F    FNV   G          E+NPFTP+A L RYWNKE+R   P+  FL SKA
Sbjct: 6   LLLQSFSFFLFNVVIVGGRTFGGGFSAEENPFTPKASLVRYWNKEIRGQSPRSEFLISKA 65

Query: 72  SPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRNF 131
           SPL AV SA F+KLAA N+L +R P FCSAANL CFPDL  SL KH+ D  F+VY  +NF
Sbjct: 66  SPLNAVDSATFSKLAAANSLPTRFPDFCSAANLFCFPDLGASLEKHDDDVKFSVYDQKNF 125

Query: 132 TTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTYA 191
           T YG+ RAGG DSFKNYS D N++ DSFRRYS  A  H D+F+ YG ++NV ++GFN+Y 
Sbjct: 126 TNYGNARAGGADSFKNYSKDGNVVTDSFRRYSRNAAGHDDKFTVYGENSNVVEEGFNSYG 185

Query: 192 SKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKRG 251
           +   GG G FT Y+ + N P  RFT+YS    GRSQ FK+Y+  ANAG+ QSF +YGK G
Sbjct: 186 TFGTGGAGDFTNYQNNVNNPTSRFTAYSDGGNGRSQTFKTYTHEANAGNGQSFTSYGKNG 245

Query: 252 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAI 311
           NG PNEF++YG ++NVIGSGFS+YG  GN ANDTFT+YG++ N PQNNF +YG  GN A+
Sbjct: 246 NGVPNEFTSYGVSSNVIGSGFSNYGESGNAANDTFTSYGSDGNVPQNNFNNYGASGNAAV 305

Query: 312 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 371
           ++F++YRD+ANVGDDSF SY+K+SNS KVNF NYG+S N GS+ F+GYG+      + FK
Sbjct: 306 DTFANYRDKANVGDDSFSSYAKDSNSEKVNFVNYGQSFNPGSETFTGYGKGAEGSKLSFK 365

Query: 372 IYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVMA 431
            Y  N+TFKDYAK+G++FA+Y NVS+  A    +G    +W+EPGKFFRES L++GTV+ 
Sbjct: 366 TYTPNSTFKDYAKKGVAFAKY-NVSTTTANTVGDGKTVNKWIEPGKFFRESSLKEGTVIP 425

Query: 432 MPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPS 491
           MPDIKDKMPKRSFLPRSI++KLPFS+SKLG +K+IFHA +NS+M  ++T+ + ECER PS
Sbjct: 426 MPDIKDKMPKRSFLPRSIITKLPFSTSKLGEIKRIFHAVENSTMGGIITDAVTECERPPS 485

Query: 492 AGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVSC 551
            GETKRCVGS EDM+DFATSVLGR+VV+R TEN  GSK  ++IG V  + GGK+TK+VSC
Sbjct: 486 VGETKRCVGSAEDMIDFATSVLGRSVVLRTTENVAGSKEKVVIGKVNGINGGKLTKAVSC 545

Query: 552 HQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGS 611
           HQSL+PYLLYYCHSVPKVRVYEA+LL+  S   INHG+AICH+DTS+W PSHGAF+ALGS
Sbjct: 546 HQSLYPYLLYYCHSVPKVRVYEADLLELNSKKKINHGIAICHMDTSSWGPSHGAFLALGS 605

Query: 612 APGRVEVCHWIFENDMTWTVAD 624
            PGR+EVCHWIFENDM W +AD
Sbjct: 606 KPGRIEVCHWIFENDMNWAIAD 626

BLAST of CmaCh12G000050 vs. TAIR10
Match: AT1G23760.1 (AT1G23760.1 BURP domain-containing protein)

HSP 1 Score: 763.1 bits (1969), Expect = 1.4e-220
Identity = 377/623 (60.51%), Postives = 466/623 (74.80%), Query Frame = 1

Query: 12  LYLSIFLLSAFNVPAAGDED----------NPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 71
           ++L  FL   ++V  AG E           NPFTP+A L RYWNK++ +  P   FL SK
Sbjct: 6   VFLLPFLSRLYHVVIAGGERLTNGGFSPEINPFTPKASLVRYWNKQIHHQSPGSAFLLSK 65

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 131
           ASPLTAV SA FAKLA++N L  RLP FCSAANL CFPDL P+L KH+ D  F+VY  RN
Sbjct: 66  ASPLTAVASATFAKLASENALPDRLPDFCSAANLFCFPDLGPTLEKHSNDVKFSVYDQRN 125

Query: 132 FTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 191
           FT YG+   GG DSFK YS + N + DSFRRYS  A  H D+F+ Y  ++NV ++ FN+Y
Sbjct: 126 FTNYGTIHPGGADSFKKYSQNGNAVTDSFRRYSRDAAGHEDKFTGYADNSNVVEEKFNSY 185

Query: 192 ASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGD-QSFANYGKR 251
            + A GG+G F  Y+   N P  RF +YS +  GR+Q FK+Y+  AN+G  QSF +YGK 
Sbjct: 186 GTSATGGSGDFKNYQSGVNNPTTRFKAYSDEGNGRAQTFKTYTHEANSGPGQSFTSYGKN 245

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 311
           GNG PNEFS+YG ++NVIGS FS+YG +GN ANDTF  YG + N PQNNF+SYG  GN A
Sbjct: 246 GNGAPNEFSSYGVSSNVIGSDFSNYGENGNAANDTFKGYGGDGNVPQNNFKSYGASGNAA 305

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +++F +YRD ANVGDDSF SY+KNSN  KV+F NYGKS N GS+ FSGYG+      + F
Sbjct: 306 VDTFLNYRDNANVGDDSFSSYAKNSNFEKVDFVNYGKSINPGSESFSGYGKGAEGNKIDF 365

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K Y  N+TFKDY K G+ FA+Y+  S GG K +VN     +WVEPGKFFRESML++GT++
Sbjct: 366 KTYTQNSTFKDYTKTGVEFAKYNRSSLGGGK-TVN-----KWVEPGKFFRESMLKEGTLI 425

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKRSFLPRSI+SKLPFS+SK+  +K++FHA DNS+ME ++T+ +RECER P
Sbjct: 426 WMPDIKDKMPKRSFLPRSIVSKLPFSTSKIAEIKRVFHANDNSTMEGIITDAVRECERPP 485

Query: 492 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           +  ETKRCVGS EDM+DFATSVLGR+VV+R TE+  GSK  +MIG V  + GG+VTKSVS
Sbjct: 486 TVSETKRCVGSAEDMIDFATSVLGRSVVLRTTESVAGSKEKVMIGKVNGINGGRVTKSVS 545

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVPKVRVYE++LLDP+S A INHG+AICH+DTSAW  +HGAF+ LG
Sbjct: 546 CHQSLYPYLLYYCHSVPKVRVYESDLLDPKSKAKINHGIAICHMDTSAWGANHGAFMLLG 605

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDM W +AD
Sbjct: 606 SRPGQIEVCHWIFENDMNWAIAD 622

BLAST of CmaCh12G000050 vs. TAIR10
Match: AT1G60390.1 (AT1G60390.1 polygalacturonase 1)

HSP 1 Score: 742.3 bits (1915), Expect = 2.5e-214
Identity = 373/623 (59.87%), Postives = 450/623 (72.23%), Query Frame = 1

Query: 12  LYLSIF-LLSAFNVPAAGDE---------DNPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 71
           L+L  F + S+ NV  AG +         +NPFTP+A L RYWN  +  D PKP+F  SK
Sbjct: 8   LFLCFFCIFSSSNVHFAGAKQTAGNITPSENPFTPKASLIRYWNNHINGDSPKPSFFLSK 67

Query: 72  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAP-SLAKHNKDSNFAVYSNR 131
           ASPLTAV S  FA LA+ + L++    FCSAA L CFP+LA  SL KH  D NFA YS +
Sbjct: 68  ASPLTAVDSTRFASLASNHALNTHHSDFCSAAKLFCFPELAAHSLEKHGDDVNFAAYSGK 127

Query: 132 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 191
           NFT YGS R  G DSFKNYS  +NI VDSFRRYS  +  H D F+ Y  + NV DQ F T
Sbjct: 128 NFTNYGSDRLSGADSFKNYSGGDNIAVDSFRRYSRNSAGHDDGFTNYAGEVNVADQSFTT 187

Query: 192 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 251
           YA+   GG+G+FT Y    N PN RFTSYS  A GRSQ F +YSEN N G QSF +Y K 
Sbjct: 188 YATGTTGGSGEFTNYNTDANEPNGRFTSYSDKANGRSQTFTTYSENGNTGYQSFTSYSKN 247

Query: 252 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 311
           GNG PNEFS YGT +NV+ +GF+ YG   NGAND+FT+YG   N P N F+ YG+GGNGA
Sbjct: 248 GNGAPNEFSGYGTGSNVVNTGFTKYGESANGANDSFTSYGENGNVPVNEFKGYGDGGNGA 307

Query: 312 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 371
           +  F +YRDQ+N+G DSF SY+KNSN+ KVNF NYGKS N+GSD F+GYGQ+    +V F
Sbjct: 308 VYGFKNYRDQSNIGVDSFSSYAKNSNNEKVNFVNYGKSFNLGSDNFTGYGQDNVGGNVSF 367

Query: 372 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 431
           K YG   +FK Y K G+ FA+YSN       +S NG    +WVE GKFFRE+ML++GT+M
Sbjct: 368 KTYGQGQSFKVYTKDGVVFARYSN------NVSSNGKTVNKWVEEGKFFREAMLKEGTLM 427

Query: 432 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 491
            MPDIKDKMPKR+FLPR+I+  LPFSSS +G + ++F A +NSSM  +++  + ECER  
Sbjct: 428 QMPDIKDKMPKRTFLPRNIVKNLPFSSSTIGEIWRVFGAGENSSMAGIISSAVSECERPA 487

Query: 492 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 551
           S GETKRCVGS EDM+DFATSVLGR VVVR TEN  GSK  ++IG V  + GG VT++VS
Sbjct: 488 SHGETKRCVGSAEDMIDFATSVLGRGVVVRTTENVVGSKKKVVIGKVNGINGGDVTRAVS 547

Query: 552 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 611
           CHQSL+PYLLYYCHSVP+VRVYE +LLDP+S   INHGVAICH+DTSAWSPSHGAF+ALG
Sbjct: 548 CHQSLYPYLLYYCHSVPRVRVYETDLLDPKSLEKINHGVAICHIDTSAWSPSHGAFLALG 607

Query: 612 SAPGRVEVCHWIFENDMTWTVAD 624
           S PG++EVCHWIFENDMTW + D
Sbjct: 608 SGPGQIEVCHWIFENDMTWNIID 624

BLAST of CmaCh12G000050 vs. TAIR10
Match: AT5G25610.1 (AT5G25610.1 BURP domain-containing protein)

HSP 1 Score: 119.8 bits (299), Expect = 6.1e-27
Identity = 72/193 (37.31%), Postives = 97/193 (50.26%), Query Frame = 1

Query: 431 KRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAPSAGETKRCVG 490
           K +FLPR     +PF S K     K F  E  S    MM +T+ ECE    +GE K C  
Sbjct: 201 KTAFLPRGEAETVPFGSEKFSETLKRFSVEAGSEEAEMMKKTIEECEARKVSGEEKYCAT 260

Query: 491 SVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGG----KVTKSVSCHQSLF 550
           S+E M+DF+ S LG+   VRA  +T+ +K N  +   K    G       KSV CH+  +
Sbjct: 261 SLESMVDFSVSKLGK-YHVRAV-STEVAKKNAPMQKYKIAAAGVKKLSDDKSVVCHKQKY 320

Query: 551 PYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALGSAPGRV 610
           P+ ++YCH      VY   L            VA+CH +TSAW+P+H AF  L   PG V
Sbjct: 321 PFAVFYCHKAMMTTVYAVPLEGENGMR--AKAVAVCHKNTSAWNPNHLAFKVLKVKPGTV 380

Query: 611 EVCHWIFENDMTW 620
            VCH++ E  + W
Sbjct: 381 PVCHFLPETHVVW 389

BLAST of CmaCh12G000050 vs. TAIR10
Match: AT1G49320.1 (AT1G49320.1 unknown seed protein like 1)

HSP 1 Score: 89.4 bits (220), Expect = 8.8e-18
Identity = 65/224 (29.02%), Postives = 101/224 (45.09%), Query Frame = 1

Query: 408 FFRESMLRKGTVMAMPDIKDKMPKRS-FLPRSILSKLPFSSSKLGVMKKIFHAEDNSSME 467
           +F  + L+ GT + +   K+ + K    L R     +PF+ SKL  +   F    +S   
Sbjct: 58  YFTLNDLKLGTKLLIYFYKNDLQKLPPLLTRQQADLIPFTKSKLDFLLDHFSITKDSPQG 117

Query: 468 TMMTETLRECERAPSAGETKRCVGSVEDMLDFATSVLGRNV--VVRATENTKGSKGNI-- 527
             + ETL  C+     GE K C  S+E ++D     +G NV   V  T+    ++ +I  
Sbjct: 118 KAIKETLGHCDAKAIEGEHKFCGTSLESLIDLVKKTMGYNVDLKVMTTKVMVPAQNSISY 177

Query: 528 ------MIGAVKAVEGGKVTKSVSCHQSLFPYLLYYCHSVPK-VRVYEAELLDPRSTANI 587
                  + A K + G    K + CH+  +PY +YYCH      RV+E  L+       +
Sbjct: 178 ALHNYTFVEAPKELVG---IKMLGCHRMPYPYAVYYCHGHKGGSRVFEVNLVTDDGRQRV 237

Query: 588 NHGVAICHLDTSAWSPSHGAFIALGSAPGRVEVCHWIFENDMTW 620
             G A+CH+DTS W   H AF  L   P    VCH+   +++ W
Sbjct: 238 V-GPAVCHMDTSTWDADHVAFKVLKMEPRSAPVCHFFPLDNIVW 277

BLAST of CmaCh12G000050 vs. NCBI nr
Match: gi|659087185|ref|XP_008444317.1| (PREDICTED: probable polygalacturonase non-catalytic subunit JP650 [Cucumis melo])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 546/623 (87.64%), Postives = 589/623 (94.54%), Query Frame = 1

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFS 60
           MHRLP   +VPLYL IF LSA NVPA GDEDNPFTP+AFL+RYWNKEVRND+PKP+FLFS
Sbjct: 1   MHRLPTLHVVPLYLFIFSLSALNVPAVGDEDNPFTPKAFLNRYWNKEVRNDIPKPSFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAA+N LSSRLP FCSAANLLCFPDL+PSLAKH++DSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N++VDSFRRYSH AL H DRFSTYG +TNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSTYGTETNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGG+GKFTTYEQS NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQAFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300
           GNGGPNEF+ YGT+ANV+GS FSSYG+DGNGANDTFTNYGN+QN+PQNNFRSYGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVVGSRFSSYGTDGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K+YGVNTTFKDYAK+GI+FA+YSNVSSGGAK SV+GSLA+RWVEPGKFFRESMLRKG+VM
Sbjct: 361 KVYGVNTTFKDYAKQGINFAKYSNVSSGGAKTSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKLGVMK+IFHA DNSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERVP 480

Query: 481 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDM+DFATSVLGRNVVVR TENTKGSKGNIMIG VK + GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVRTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE +LLDP S A INHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPASKATINHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of CmaCh12G000050 vs. NCBI nr
Match: gi|449450462|ref|XP_004142981.1| (PREDICTED: probable polygalacturonase non-catalytic subunit JP650 [Cucumis sativus])

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 541/623 (86.84%), Postives = 586/623 (94.06%), Query Frame = 1

Query: 1   MHRLPAPLLVPLYLSIFLLSAFNVPAAGDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFS 60
           MHRLP   +VPLYL IF LS+ NV  AGDED+PFTP+AFL+RYWNKEVRND PKP+FLFS
Sbjct: 1   MHRLPTLYVVPLYLFIFSLSSLNVSTAGDEDDPFTPKAFLNRYWNKEVRNDFPKPSFLFS 60

Query: 61  KASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNR 120
           KASPLTAVQSAAFAKLAA+N LSSRLP FCSAANLLCFPDL+PSLAKH++DSNFAVYSN+
Sbjct: 61  KASPLTAVQSAAFAKLAAENALSSRLPDFCSAANLLCFPDLSPSLAKHDRDSNFAVYSNK 120

Query: 121 NFTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNT 180
           NFTTYG++R+GGIDSFKNYSN +N++VDSFRRYSH AL H DRFS YG DTNVPDQ FNT
Sbjct: 121 NFTTYGTQRSGGIDSFKNYSNGDNVVVDSFRRYSHEALGHTDRFSVYGTDTNVPDQSFNT 180

Query: 181 YASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKR 240
           YASKAIGG+GKFTTYEQS NVPNLRFT+YS+DATGRSQ FKSYSENANAGDQSF NYGKR
Sbjct: 181 YASKAIGGDGKFTTYEQSVNVPNLRFTTYSSDATGRSQSFKSYSENANAGDQSFVNYGKR 240

Query: 241 GNGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGA 300
           GNGGPNEF+ YGT+ANVIGS FSSYG++GNGANDTFTNYGN+QN+PQNNFRSYGEGGNGA
Sbjct: 241 GNGGPNEFTGYGTSANVIGSRFSSYGAEGNGANDTFTNYGNDQNNPQNNFRSYGEGGNGA 300

Query: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGF 360
           IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFA+YGKS N G+DKFSGYGQNGN QSVGF
Sbjct: 301 IESFSSYRDQANVGDDSFQSYSKNSNSAKVNFASYGKSFNEGTDKFSGYGQNGNGQSVGF 360

Query: 361 KIYGVNTTFKDYAKRGISFAQYSNVSSGGAKMSVNGSLAQRWVEPGKFFRESMLRKGTVM 420
           K YGVNTTFKDYAK+GI+FA+YSNVSSGGAKMSV+GSLA+RWVEPGKFFRESMLRKG+VM
Sbjct: 361 KTYGVNTTFKDYAKQGINFAKYSNVSSGGAKMSVSGSLAKRWVEPGKFFRESMLRKGSVM 420

Query: 421 AMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERAP 480
           AMPDI+DKMPKRSFLPRSILSKLPFSSSKLGVMK+IFHA DNSSMETMMTET+RECER P
Sbjct: 421 AMPDIRDKMPKRSFLPRSILSKLPFSSSKLGVMKQIFHAGDNSSMETMMTETVRECERLP 480

Query: 481 SAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSVS 540
           SAGETKRCVGSVEDM+DFATSVLGRNVVV+ TENTKGSKGNIMIG VK + GGKVT+SVS
Sbjct: 481 SAGETKRCVGSVEDMIDFATSVLGRNVVVQTTENTKGSKGNIMIGKVKGMNGGKVTESVS 540

Query: 541 CHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIALG 600
           CHQSLFPYLLYYCHSVPKVRVYE +LLDP + A +NHGVAICHLDTSAWSP+HGAFIALG
Sbjct: 541 CHQSLFPYLLYYCHSVPKVRVYEVDLLDPATKATVNHGVAICHLDTSAWSPAHGAFIALG 600

Query: 601 SAPGRVEVCHWIFENDMTWTVAD 624
           SAPGRVEVCHWIFENDMTWTVAD
Sbjct: 601 SAPGRVEVCHWIFENDMTWTVAD 623

BLAST of CmaCh12G000050 vs. NCBI nr
Match: gi|694372402|ref|XP_009363553.1| (PREDICTED: probable polygalacturonase non-catalytic subunit JP650 isoform X2 [Pyrus x bretschneideri])

HSP 1 Score: 868.6 bits (2243), Expect = 6.5e-249
Identity = 423/624 (67.79%), Postives = 512/624 (82.05%), Query Frame = 1

Query: 8   LLVPLYLSIFLLSAFNVPAA------GDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 67
           L+ PL L     S+ NV  A      G  +NPF+P+A+L RYWNKEV+ND  KP+FL SK
Sbjct: 9   LVTPLSLFFIFFSSLNVAIAAAGGSSGATENPFSPKAYLVRYWNKEVQNDAEKPSFLLSK 68

Query: 68  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 127
           ASPL AV SAAFAKLAAQN LS+RLP+FCSAANLLCFPDLA SL KH+KD+NFAVY+++N
Sbjct: 69  ASPLDAVGSAAFAKLAAQNGLSTRLPEFCSAANLLCFPDLAQSLEKHDKDANFAVYADKN 128

Query: 128 FTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 187
           FT YG+ R  G+DSFKNYS  EN+ VDSFRRYS  +++H D+F++Y  D NV DQ FN Y
Sbjct: 129 FTNYGTDRLAGVDSFKNYSLGENLPVDSFRRYSRDSVNHKDQFTSYANDGNVVDQSFNGY 188

Query: 188 ASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRG 247
           A+ A GG G F  Y  S NVP+L F SYS D+ GRS  F  YSENANAGDQSF +Y K G
Sbjct: 189 AAGATGGAGDFKKYADSVNVPHLGFNSYSDDSNGRSHGFTGYSENANAGDQSFTSYSKNG 248

Query: 248 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAI 307
           NG PNEF+ YGTNANV+GSGF++YG  GNGAND F NYGN+QNDP NNF+SYG+GGN  +
Sbjct: 249 NGAPNEFTGYGTNANVVGSGFTAYGQSGNGANDKFNNYGNDQNDPANNFKSYGDGGNAGV 308

Query: 308 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 367
           E+F+SYR++ANVGDDSFQSY+KNSN  K++F +YGKS N+GSDKF+GYG+    Q +GFK
Sbjct: 309 ETFTSYRERANVGDDSFQSYAKNSNGEKMDFTSYGKSFNIGSDKFTGYGKGARGQDIGFK 368

Query: 368 IYGVNTTFKDYAKR-GISFAQYSNVSSGGAK-MSVNGSLAQRWVEPGKFFRESMLRKGTV 427
           IYGVNTTFKDYAK+  +SF  Y+   +G  K  + +GS+ ++WVEPGKFFRESML+KG V
Sbjct: 369 IYGVNTTFKDYAKKETVSFKSYTKAGAGSVKAAAASGSMVKKWVEPGKFFRESMLKKGVV 428

Query: 428 MAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERA 487
           M MPDI+DKMPKRSFLPR+ILSKLPF++SK+  +K+IFHA DNS+ME ++ + L+ECERA
Sbjct: 429 MPMPDIQDKMPKRSFLPRTILSKLPFATSKIAALKQIFHAADNSTMEKIIVDALQECERA 488

Query: 488 PSAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSV 547
           PSAGETKRCVGS EDM+DFAT++LGRN+VVR+TEN  G+K ++MIG+VK + GGKVT+SV
Sbjct: 489 PSAGETKRCVGSAEDMIDFATTILGRNIVVRSTENVNGAKHDVMIGSVKGINGGKVTQSV 548

Query: 548 SCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIAL 607
           SCHQSLFPYLLYYCHSVPKVRVYEA+LLDP S A +NHGVAICHLDTSAWSP+HG+F+AL
Sbjct: 549 SCHQSLFPYLLYYCHSVPKVRVYEADLLDPVSKAKVNHGVAICHLDTSAWSPTHGSFLAL 608

Query: 608 GSAPGRVEVCHWIFENDMTWTVAD 624
           GS PGR+EVCHWIF+NDMTWT+AD
Sbjct: 609 GSGPGRIEVCHWIFQNDMTWTIAD 632

BLAST of CmaCh12G000050 vs. NCBI nr
Match: gi|657994670|ref|XP_008389645.1| (PREDICTED: probable polygalacturonase non-catalytic subunit JP650 [Malus domestica])

HSP 1 Score: 868.6 bits (2243), Expect = 6.5e-249
Identity = 424/624 (67.95%), Postives = 510/624 (81.73%), Query Frame = 1

Query: 8   LLVPLYLSIFLLSAFNVPAA------GDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 67
           L+ PL L     S+ NV  A      G  +NPF+P+A+L RYWNKEVRND  KP+FL SK
Sbjct: 9   LVTPLSLFFIFFSSLNVAVAAAGGSSGAAENPFSPKAYLVRYWNKEVRNDAEKPSFLLSK 68

Query: 68  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 127
           ASPL  V SAAFAKLAAQN LS+RLP+FCSAANLLCFPDLA SL KH+KD+NFA+Y+++N
Sbjct: 69  ASPLNTVDSAAFAKLAAQNGLSTRLPEFCSAANLLCFPDLAQSLEKHDKDANFAIYADKN 128

Query: 128 FTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 187
           FT YG+ R  G+DSFKNYS   NI VDSFRRYS  +++H D+F++Y  D NV DQ FN Y
Sbjct: 129 FTNYGTDRLAGVDSFKNYSLGNNIPVDSFRRYSRDSVNHKDQFTSYANDGNVVDQSFNGY 188

Query: 188 ASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRG 247
           A+ A GG G F  Y  S NVP+L F SYS D+ GRS  F  YSENANAGDQSF  Y K G
Sbjct: 189 AAGATGGAGDFKKYADSVNVPHLGFNSYSDDSNGRSHGFTGYSENANAGDQSFTGYSKNG 248

Query: 248 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAI 307
           NG PNEF+ YG+NANV+GSGF+SYG  GNGAND F+NYGN+QN+P+NNF+SYG+GGN  +
Sbjct: 249 NGAPNEFTGYGSNANVVGSGFTSYGQSGNGANDKFSNYGNDQNNPKNNFQSYGDGGNAGV 308

Query: 308 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 367
           E+F+SYR++ANVGDDSFQSY+KNSN  K++F +YGKS N+GSDKF+GYG+    Q +GFK
Sbjct: 309 ETFTSYRERANVGDDSFQSYAKNSNGEKMDFTSYGKSFNIGSDKFTGYGKGAKGQDIGFK 368

Query: 368 IYGVNTTFKDYAKR-GISFAQYSNVSSGGAK-MSVNGSLAQRWVEPGKFFRESMLRKGTV 427
           IYGVNTTFKDYAK+  +SF  Y+    G  K  + +GSL ++WVEPGKFFRESML+KG V
Sbjct: 369 IYGVNTTFKDYAKKETVSFKSYTKAGGGSVKAAAASGSLVKKWVEPGKFFRESMLKKGVV 428

Query: 428 MAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRECERA 487
           M MPDI+DKMPKRSFLPR+ILSKLPF++SK+  +K+IFHA DNSSME ++ + L+ECERA
Sbjct: 429 MPMPDIQDKMPKRSFLPRTILSKLPFATSKIATLKQIFHAADNSSMEKIIVDALQECERA 488

Query: 488 PSAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKVTKSV 547
           PSAGETKRCVGS EDM+DFAT++LGRNVVVR+TEN  G+K ++M+G+VK + GGKVT+SV
Sbjct: 489 PSAGETKRCVGSAEDMIDFATTILGRNVVVRSTENVNGAKHDVMVGSVKGINGGKVTQSV 548

Query: 548 SCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGAFIAL 607
           SCHQSLFPYLLYYCHSVPKVRVYEA+LLDP S A INHGVAICHLDTSAWSP+HG+F+AL
Sbjct: 549 SCHQSLFPYLLYYCHSVPKVRVYEADLLDPVSKAKINHGVAICHLDTSAWSPTHGSFLAL 608

Query: 608 GSAPGRVEVCHWIFENDMTWTVAD 624
           GS PGR+EVCHWIF+NDMTWT+AD
Sbjct: 609 GSGPGRIEVCHWIFQNDMTWTIAD 632

BLAST of CmaCh12G000050 vs. NCBI nr
Match: gi|658009623|ref|XP_008340027.1| (PREDICTED: probable polygalacturonase non-catalytic subunit JP650 [Malus domestica])

HSP 1 Score: 863.2 bits (2229), Expect = 2.7e-247
Identity = 424/628 (67.52%), Postives = 509/628 (81.05%), Query Frame = 1

Query: 8   LLVPLYLSIFLLSAFNVPAA------GDEDNPFTPRAFLSRYWNKEVRNDLPKPTFLFSK 67
           L+ PL L     S+ NV  A      G  +NPF+P+A+L RYW+KEVRND  KP+FL SK
Sbjct: 9   LVTPLSLFFIFFSSLNVALAANGGSSGAAENPFSPKAYLVRYWSKEVRNDAEKPSFLLSK 68

Query: 68  ASPLTAVQSAAFAKLAAQNTLSSRLPQFCSAANLLCFPDLAPSLAKHNKDSNFAVYSNRN 127
           ASPL AV SAAFAKLAAQN LS+RLP+FCSAANLLCFPDLAPSL KH+K++NFAVY+++N
Sbjct: 69  ASPLNAVDSAAFAKLAAQNGLSTRLPEFCSAANLLCFPDLAPSLEKHDKNANFAVYTDKN 128

Query: 128 FTTYGSKRAGGIDSFKNYSNDENILVDSFRRYSHGALSHADRFSTYGIDTNVPDQGFNTY 187
           FT YG+ R  G+DSFKNYS  EN+ VDSFRRYS  +++H D+F++Y  D NV DQ FN+Y
Sbjct: 129 FTNYGTDRLAGVDSFKNYSIGENLPVDSFRRYSRDSVNHKDQFTSYANDGNVVDQSFNSY 188

Query: 188 ASKAIGGNGKFTTYEQSTNVPNLRFTSYSTDATGRSQVFKSYSENANAGDQSFANYGKRG 247
           A  + GG G F  Y  S NVP+L F SYS D+ GRS  F  YSENANAGDQSF++Y K  
Sbjct: 189 AGGSTGGAGDFKKYADSVNVPHLGFNSYSDDSNGRSHGFTGYSENANAGDQSFSSYSKNA 248

Query: 248 NGGPNEFSAYGTNANVIGSGFSSYGSDGNGANDTFTNYGNEQNDPQNNFRSYGEGGNGAI 307
           NG PNEF+ YGT+ANV+GSGF+ YG  GNGAND F NYGN+QN+P NNFRSYG+GGN  +
Sbjct: 249 NGAPNEFTGYGTSANVVGSGFTGYGQSGNGANDKFNNYGNDQNNPTNNFRSYGDGGNAGV 308

Query: 308 ESFSSYRDQANVGDDSFQSYSKNSNSAKVNFANYGKSANVGSDKFSGYGQNGNAQSVGFK 367
           E+F+SYRD ANVG DSFQSY+KNSN  K++F NYGKS NVGSDKF+GYGQ    Q +GFK
Sbjct: 309 ETFTSYRDSANVGXDSFQSYAKNSNGQKMDFTNYGKSFNVGSDKFTGYGQGAKGQEIGFK 368

Query: 368 IYGVNTTFKDYAKR-GISFAQYSNVSSGGAKM-----SVNGSLAQRWVEPGKFFRESMLR 427
           IYG NTTFKDYAK+  +SF  Y+   +G  K+     + +GSL ++WVEPGKFFRESML+
Sbjct: 369 IYGANTTFKDYAKKETVSFKSYTKAGAGSEKVAATMAAASGSLVKKWVEPGKFFRESMLK 428

Query: 428 KGTVMAMPDIKDKMPKRSFLPRSILSKLPFSSSKLGVMKKIFHAEDNSSMETMMTETLRE 487
           KG VM MPDI+DK PKRSFLPR+ILSKLPF++SK+  +K+IFHA DNS+ME ++ + L+E
Sbjct: 429 KGVVMPMPDIQDKTPKRSFLPRTILSKLPFATSKIAALKQIFHAADNSTMEKIIVDALQE 488

Query: 488 CERAPSAGETKRCVGSVEDMLDFATSVLGRNVVVRATENTKGSKGNIMIGAVKAVEGGKV 547
           CERAPSAGETKRCVGS EDM+DFAT+VLGRNVVVR+TEN  G+K ++MIG+VK + GGKV
Sbjct: 489 CERAPSAGETKRCVGSAEDMIDFATTVLGRNVVVRSTENVNGAKHDVMIGSVKGINGGKV 548

Query: 548 TKSVSCHQSLFPYLLYYCHSVPKVRVYEAELLDPRSTANINHGVAICHLDTSAWSPSHGA 607
           T+SVSCHQS+FPYLLYYCHSVPKVRVYEA+LLDP S A INHGVAICHLDTSAWSP+HG+
Sbjct: 549 TQSVSCHQSMFPYLLYYCHSVPKVRVYEADLLDPVSKAKINHGVAICHLDTSAWSPTHGS 608

Query: 608 FIALGSAPGRVEVCHWIFENDMTWTVAD 624
           F+ALGS PGR+EVCHWIF+NDMTWT+AD
Sbjct: 609 FMALGSGPGRIEVCHWIFQNDMTWTIAD 636