CmaCh11G012350 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh11G012350
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionNicotianamine synthase
LocationCma_Chr11 : 8123732 .. 8125093 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
NNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCTGCTTTCCTTTAACCTCATAGTGTCGACATGGTTAGCGAAGAAGAAGCTCTAATCCAAAAGGTGGTGGATTTGTATGAGCAAATCTCTGGCCTTGAAAGCCTCAAACCTTGTAAGCATGTCGACACTCTGTTCACAGAGTTGGTGGTCACGTGCATGCCACCCACCAGCCCTAATTTCCACATCAATTCACTCTCAAACCCTCTTCAACAAATGAGATCAAATCTGATCCGCCTTTGCGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAATTTTGATTACCCTCTTCACCATCTCCATCTCTTCCCTTACTTCTCCAATTATCTCAAGCTTAGCCTCCTTGAATTCTCCATCCTCCGCCGCCACTGCCCCATCCTCCCCTCCTCCGTTGCCTTCGTCGGCTCCGGCCCTCTTCCTCTCACCTCCATCGTCCTCGCCACCCGCCACCTCCCCTCCACCTCCTTCCACAATTATGATCTCGACCCACTTGCTAATTCCCAGGTATCAGGCCTTAAAGTAGATGTGAACATACGTGATTTCTTAGTTTATTTTCTCATACTTGTTTGTTGTTGTGCAGGCGTCAAAATTGGTATCGGTGGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACGGAGGAGTTGAAGGAGTACGAGGTTGTGTTTTTGGCAGCTCTAGTAGGAATGGAGAAGGAGGAGAAATTGAGAGTGATAGAGCATTTGTGTGAGTTCATGGCGGAAGGGGCTTGTTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTCCTTTATCCTGTGGTGGAGGAATGTGATTTGGTAGGGTTTGAGGTTCTATCGGTGTTTCATCCGAGTGATGAAGTTATAAACTCAGTGATAATTGCAAGAAAAAAGAAGAAGGATGATGATTGTTTGAAGATTGTTGGTGATGATAATCATGAAAATGGTATTGAAAATGGTGTTGTTGTTCATAACAAACAATGTTGTGATCACATTCATGGCTTCAACAATCATGGAGGAATGATTGAGGAGTTAGCTGAGTAGTAAATGATGTTAAGCTTATGTGGGTTTCTGATTATGTTCACGTTTTAGGGCTAATTTTAGGGCAAATGAACCACCCTTCACTTATGTTGGTTCATGCTTTATGTAATAATTTTTGTGTTCTCATTTATGAATGAATGAGAATGCTATGTTTTTTTGTTTTACCATTTCGATCCTATCCGATCCATTCGACTATGTTTTTCTCGATTGCTATAAAACTATTTTAAAGTTTCAATAA

mRNA sequence

NNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCTGCTTTCCTTTAACCTCATAGTGTCGACATGGTTAGCGAAGAAGAAGCTCTAATCCAAAAGGTGGTGGATTTGTATGAGCAAATCTCTGGCCTTGAAAGCCTCAAACCTTGTAAGCATGTCGACACTCTGTTCACAGAGTTGGTGGTCACGTGCATGCCACCCACCAGCCCTAATTTCCACATCAATTCACTCTCAAACCCTCTTCAACAAATGAGATCAAATCTGATCCGCCTTTGCGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAATTTTGATTACCCTCTTCACCATCTCCATCTCTTCCCTTACTTCTCCAATTATCTCAAGCTTAGCCTCCTTGAATTCTCCATCCTCCGCCGCCACTGCCCCATCCTCCCCTCCTCCGTTGCCTTCGTCGGCTCCGGCCCTCTTCCTCTCACCTCCATCGTCCTCGCCACCCGCCACCTCCCCTCCACCTCCTTCCACAATTATGATCTCGACCCACTTGCTAATTCCCAGGCGTCAAAATTGGTATCGGTGGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACGGAGGAGTTGAAGGAGTACGAGGTTGTGTTTTTGGCAGCTCTAGTAGGAATGGAGAAGGAGGAGAAATTGAGAGTGATAGAGCATTTGTGTGAGTTCATGGCGGAAGGGGCTTGTTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTCCTTTATCCTGTGGTGGAGGAATGTGATTTGGTAGGGTTTGAGGTTCTATCGGTGTTTCATCCGAGTGATGAAGTTATAAACTCAGTGATAATTGCAAGAAAAAAGAAGAAGGATGATGATTGTTTGAAGATTGTTGGTGATGATAATCATGAAAATGGTATTGAAAATGGTGTTGTTGTTCATAACAAACAATGTTGTGATCACATTCATGGCTTCAACAATCATGGAGGAATGATTGAGGAGTTAGCTGAGTAGTAAATGATGTTAAGCTTATGTGGGTTTCTGATTATGTTCACGTTTTAGGGCTAATTTTAGGGCAAATGAACCACCCTTCACTTATGTTGGTTCATGCTTTATGTAATAATTTTTGTGTTCTCATTTATGAATGAATGAGAATGCTATGTTTTTTTGTTTTACCATTTCGATCCTATCCGATCCATTCGACTATGTTTTTCTCGATTGCTATAAAACTATTTTAAAGTTTCAATAA

Coding sequence (CDS)

ATGGTTAGCGAAGAAGAAGCTCTAATCCAAAAGGTGGTGGATTTGTATGAGCAAATCTCTGGCCTTGAAAGCCTCAAACCTTGTAAGCATGTCGACACTCTGTTCACAGAGTTGGTGGTCACGTGCATGCCACCCACCAGCCCTAATTTCCACATCAATTCACTCTCAAACCCTCTTCAACAAATGAGATCAAATCTGATCCGCCTTTGCGGCCAAGCTGAGTCCCTTTTGGAGTCCCATTTCTCTGATCTCTTAGCCAATTTTGATTACCCTCTTCACCATCTCCATCTCTTCCCTTACTTCTCCAATTATCTCAAGCTTAGCCTCCTTGAATTCTCCATCCTCCGCCGCCACTGCCCCATCCTCCCCTCCTCCGTTGCCTTCGTCGGCTCCGGCCCTCTTCCTCTCACCTCCATCGTCCTCGCCACCCGCCACCTCCCCTCCACCTCCTTCCACAATTATGATCTCGACCCACTTGCTAATTCCCAGGCGTCAAAATTGGTATCGGTGGACTCGGACCTGAAGACGAGGATGGTGTTTCACACGTGCGACATCATGAAAGTGACGGAGGAGTTGAAGGAGTACGAGGTTGTGTTTTTGGCAGCTCTAGTAGGAATGGAGAAGGAGGAGAAATTGAGAGTGATAGAGCATTTGTGTGAGTTCATGGCGGAAGGGGCTTGTTTGATGGTGAGAAGTGCTCATGGAGGAAGGGCTTTCCTTTATCCTGTGGTGGAGGAATGTGATTTGGTAGGGTTTGAGGTTCTATCGGTGTTTCATCCGAGTGATGAAGTTATAAACTCAGTGATAATTGCAAGAAAAAAGAAGAAGGATGATGATTGTTTGAAGATTGTTGGTGATGATAATCATGAAAATGGTATTGAAAATGGTGTTGTTGTTCATAACAAACAATGTTGTGATCACATTCATGGCTTCAACAATCATGGAGGAATGATTGAGGAGTTAGCTGAGTAG

Protein sequence

MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQQMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCPILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVHNKQCCDHIHGFNNHGGMIEELAE
Homology
[Close]
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match: NAS4_ARATH (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.0e-92
Identity = 169/276 (61.23%), Postives = 222/276 (80.43%), Query Frame = 1 

Query: 4   EEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQQMR 63
           +++ L+ K+ DLYE+IS LE+LKPC+ VDTLF +LV TC+PP +PN  +  +S  +Q+MR
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPP-NPNIDVTKMSENIQEMR 64

Query: 64  SNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP-I 123
           SNLI++CG+AE  LE HFS +L +F D PLHHL+LFPY++NYLKLS LEF +L ++    
Sbjct: 65  SNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGF 124

Query: 124 LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVFH 183
           +P +VAF+GSGPLPLTS+VLA+ HL  + FHN+D+DP AN  A++LVS D DL  RM FH
Sbjct: 125 VPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFH 184

Query: 184 TCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFLY 243
           T DIM VTE LK ++VVFLAALVGM+K+EK++V+EHL + M+ GA LM+RSAHG RAFLY
Sbjct: 185 TVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLY 244

Query: 244 PVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKD 278
           P+VE CDL GFEVLSV+HP+DEVINS++I+RK  +D
Sbjct: 245 PIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGED 279

[Close]
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match: NAS_SOLLC (Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.5e-92
Identity = 177/317 (55.84%), Postives = 235/317 (74.13%), Query Frame = 1 

Query: 8   LIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQQMRSNLI 67
           +++KV +LYEQIS LE+L P K V+ LFT+LV TCMPP      ++ L   +Q++RS+LI
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP--IDVSKLCQKIQEIRSHLI 68

Query: 68  RLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCPILPSSVA 127
           +LCGQAE LLESHFS +L++++ PL HLH+FPYF NY+KLSLLE++IL ++   +P  +A
Sbjct: 69  KLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIA 128

Query: 128 FVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVFHTCDIMK 187
           F+GSGPLPLTS+VLAT+HL +T FHNYD+D  AN  AS LV+ D D+ +RM FHT D+M 
Sbjct: 129 FIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMD 188

Query: 188 VTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFLYPVVEEC 247
           VT  LK+Y+VVFLAALVGM+KE+K++V++HL ++M+ GA LM+RSAHG RAFLYPV++  
Sbjct: 189 VTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPR 248

Query: 248 DLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVHNKQCCDH 307
           DL GFEVLSV+HP+DEVINSVIIARK       L         +G+    V+ +K  C  
Sbjct: 249 DLRGFEVLSVYHPTDEVINSVIIARKLPVPSVPLL--------DGL-GAYVLPSKCACAE 308

Query: 308 IHGFN--NHGGMIEELA 323
           IH FN  N   ++EE A
Sbjct: 309 IHAFNPLNKMNLVEEFA 314

[Close]
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match: NAS2_ARATH (Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 3.2e-90
Identity = 174/325 (53.54%), Postives = 232/325 (71.38%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNP-L 60
           M  E   ++++++DLY QIS LESLKP K+VDTLF +LV TC+P T  N  +  + +  +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP-TDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRH 120
           + MRS+LI+LCG+AE  LE HFS +L +F D PL+HLH+FPY++NYLKL  LEF +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRM 180
              +P+ VAF+GSGP+PLTSIVLA  HLP+T+FHN+D+D  AN+ AS LVS DSDL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRA 240
           +FHT D++   E L +Y+VVFLAALVGM+KE K++ IEHL + MA GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVV 300
           FLYP+V+ CDL GFEVL+++HPSD+V+NSV+IARK          +G  N   G + G  
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK----------LGGSNGARGSQIGRC 300

Query: 301 VHNKQCCDHIHGFNNHGGMIEELAE 324
           V     C  +H   N+ GM + L E
Sbjct: 301 VVMPCNCSKVHAILNNRGMEKNLIE 314

[Close]
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match: NAS3_ARATH (Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-88
Identity = 167/275 (60.73%), Postives = 216/275 (78.55%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           M  ++E L+Q + DLYE+IS LESLKP + V+ LF +LV TC+PP +PN  +  + + +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPP-NPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRHC 120
           ++R NLI++CG AE  LE+HFS +L ++ D PLHHL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 P-ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRM 180
              +P SVAF+GSGPLPLTSIVLA+ HL  T FHN+D+DP ANS AS LVS D D+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK++VIEHL + MA GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARK 274
           FLYP+VE CDL GFEVLS++HP+D+VINSV+I++K
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match: NAS1_ARATH (Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.1e-85
Identity = 155/276 (56.16%), Postives = 211/276 (76.45%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           M  +   ++++++DLY+QIS L+SLKP K+VDTLF +LV TC+P T  N  + ++   ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP-TDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDY---PLHHLHLFPYFSNYLKLSLLEFSILRR 120
            MR+NLI+LCG+AE  LE HFS +L +      PL HLH+FPY+SNYLKL  LEF +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTR 180
           H   +P+ +AFVGSGP+PLTSIVLA  HLP+T+FHN+D+D  AN+ AS LVS D DL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGR 240
           M+FHT D++  TE L +Y+VVFLAALVGM+KE K++ IEHL + MA GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARK 274
           AFLYP+V+  DL GF++L+++HP+D+V+NSV+IARK
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of CmaCh11G012350 vs. TrEMBL
Match: A0A0A0LVX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G561410 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 6.2e-133
Identity = 253/336 (75.30%), Postives = 280/336 (83.33%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180
             LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+ANS+AS LVS D DLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK-------------DDDCLKIVGD 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K             D+ C   V  
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DNHENGIENGVVVHNKQCCDHIHGFNNHGGMIEELA 323
           D +EN   +  VVHN +C +  +GFNNHGG IEE A
Sbjct: 301 DENENVKISSGVVHNNKCSEIQNGFNNHGGKIEEFA 336

[Close]
BLAST of CmaCh11G012350 vs. TrEMBL
Match: I1NBR5_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_19G228400 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.5e-102
Identity = 202/324 (62.35%), Postives = 246/324 (75.93%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV  LYEQIS LESLKPCK+VD LFT+LV+TC+PP+     +  L+  +Q
Sbjct: 1   MVCEEELLIEKVCSLYEQISSLESLKPCKNVDMLFTKLVLTCIPPSP--IDVTKLNKTVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
            +RS+LI+LCG+AE  LESH+S +L ++D PL+HL++FPY+SNYLKL  LEF+IL +HC 
Sbjct: 61  DIRSHLIKLCGEAEGHLESHYSTILGSYDKPLNHLNIFPYYSNYLKLGHLEFTILSQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +P+ VAFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A +LVS D DL  RM F
Sbjct: 121 HVPTKVAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNAMRLVSSDPDLSKRMFF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI++V+  LKEYEVV+LAALVGM+KE K R+I+HL + M  GA LM+RSAHG RAFL
Sbjct: 181 HTNDILEVSNALKEYEVVYLAALVGMDKEGKNRIIDHLGKHMNPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPVVE CD  GFEVLSVFHPSDEVINSV+IARK              + E G+   +++ 
Sbjct: 241 YPVVEPCDFRGFEVLSVFHPSDEVINSVVIARKLY-----------HSLEQGLVGSMILP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK  C  I  FN  NHG MIEELA
Sbjct: 301 NK--CSEIQAFNPLNHGNMIEELA 309

[Close]
BLAST of CmaCh11G012350 vs. TrEMBL
Match: A0A0B2SE88_GLYSO (Nicotianamine synthase OS=Glycine soja GN=glysoja_001928 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.5e-102
Identity = 202/324 (62.35%), Postives = 246/324 (75.93%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV  LYEQIS LESLKPCK+VD LFT+LV+TC+PP+     +  L+  +Q
Sbjct: 1   MVCEEELLIEKVCSLYEQISSLESLKPCKNVDMLFTKLVLTCIPPSP--IDVTKLNKTVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
            +RS+LI+LCG+AE  LESH+S +L ++D PL+HL++FPY+SNYLKL  LEF+IL +HC 
Sbjct: 61  DIRSHLIKLCGEAEGHLESHYSTILGSYDKPLNHLNIFPYYSNYLKLGHLEFTILSQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +P+ VAFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A +LVS D DL  RM F
Sbjct: 121 HVPTKVAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNAMRLVSSDPDLSKRMFF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI++V+  LKEYEVV+LAALVGM+KE K R+I+HL + M  GA LM+RSAHG RAFL
Sbjct: 181 HTNDILEVSNALKEYEVVYLAALVGMDKEGKNRIIDHLGKHMNPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPVVE CD  GFEVLSVFHPSDEVINSV+IARK              + E G+   +++ 
Sbjct: 241 YPVVEPCDFRGFEVLSVFHPSDEVINSVVIARKLY-----------HSLEQGLVGSMILP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK  C  I  FN  NHG MIEELA
Sbjct: 301 NK--CSEIQAFNPLNHGNMIEELA 309

[Close]
BLAST of CmaCh11G012350 vs. TrEMBL
Match: A0A151TW62_CAJCA (Nicotianamine synthase OS=Cajanus cajan GN=KK1_010551 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.7e-102
Identity = 201/324 (62.04%), Postives = 251/324 (77.47%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV +LYEQIS LESLKP K+VD LFT+LV+TC+PP+     +  L   +Q
Sbjct: 1   MVCEEEMLIEKVCELYEQISSLESLKPSKNVDMLFTQLVLTCIPPSP--IDVTKLDKTVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           ++RS+LIRLCG+AE  LE+H+S +L ++D PL HL++FPY+SNYLKL LLEF+IL +HC 
Sbjct: 61  EIRSHLIRLCGEAEGHLETHYSTILGSYDNPLDHLNIFPYYSNYLKLGLLEFTILSQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +P+ +AFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A++LVS D DL  RMVF
Sbjct: 121 HVPTKIAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNATRLVSSDPDLSKRMVF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI+ V+  LKEYEVV+LAALVGM+KE K R+I+HL + MA GA LM+RSAHG RAFL
Sbjct: 181 HTTDILDVSNGLKEYEVVYLAALVGMDKEGKNRIIDHLAKNMAPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPVVE CDL GFEVLSVFHP+DEVINSV+IARK  +      +      + G+ + +++ 
Sbjct: 241 YPVVEPCDLRGFEVLSVFHPTDEVINSVVIARKYPQ-----PVPMHSLDQQGLAS-MILP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK  C  I  FN  NHG +IEELA
Sbjct: 301 NK--CSEIQAFNPLNHGNIIEELA 314

[Close]
BLAST of CmaCh11G012350 vs. TrEMBL
Match: A0A0S3RZV4_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G374000 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 1.6e-101
Identity = 198/324 (61.11%), Postives = 247/324 (76.23%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV  LYEQIS LESLKPCK+VD LFT+LV+TC+PP+     + +L+  +Q
Sbjct: 1   MVCEEELLIEKVCGLYEQISSLESLKPCKNVDMLFTQLVLTCIPPSP--IDVTNLNKNVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           ++RS+LI+LCG+AE  LE+H+S LL ++D PL +LH+FPY+SNYLKL LLEF+IL +HC 
Sbjct: 61  EIRSHLIKLCGEAEGHLETHYSTLLGSYDNPLQNLHIFPYYSNYLKLGLLEFTILTQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +PS +AFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A +LVS   DL  RMVF
Sbjct: 121 HVPSKIAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNAMRLVSSHPDLSNRMVF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI+ V+  LKEYEVV+LAALVGM+KE K R+I+HL + M  GA LM+RSAHG RAFL
Sbjct: 181 HTTDILDVSNALKEYEVVYLAALVGMDKESKNRIIDHLAKHMNPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPV+E CDL GFEVL+VFHP+DEVINSV+IARK          V   + + G+ + ++ +
Sbjct: 241 YPVIEPCDLRGFEVLTVFHPTDEVINSVVIARKYP--------VPMHSLDQGLASKILPN 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
               C  I  FN  NHG MIEE A
Sbjct: 301 K---CSEIQAFNPLNHGNMIEEFA 311

[Close]
BLAST of CmaCh11G012350 vs. TAIR10
Match: AT1G56430.1 (AT1G56430.1 nicotianamine synthase 4)

HSP 1 Score: 340.5 bits (872), Expect = 1.1e-93
Identity = 169/276 (61.23%), Postives = 222/276 (80.43%), Query Frame = 1 

Query: 4   EEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQQMR 63
           +++ L+ K+ DLYE+IS LE+LKPC+ VDTLF +LV TC+PP +PN  +  +S  +Q+MR
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPP-NPNIDVTKMSENIQEMR 64

Query: 64  SNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP-I 123
           SNLI++CG+AE  LE HFS +L +F D PLHHL+LFPY++NYLKLS LEF +L ++    
Sbjct: 65  SNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGF 124

Query: 124 LPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVFH 183
           +P +VAF+GSGPLPLTS+VLA+ HL  + FHN+D+DP AN  A++LVS D DL  RM FH
Sbjct: 125 VPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFH 184

Query: 184 TCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFLY 243
           T DIM VTE LK ++VVFLAALVGM+K+EK++V+EHL + M+ GA LM+RSAHG RAFLY
Sbjct: 185 TVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLY 244

Query: 244 PVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKD 278
           P+VE CDL GFEVLSV+HP+DEVINS++I+RK  +D
Sbjct: 245 PIVEPCDLEGFEVLSVYHPTDEVINSIVISRKLGED 279

[Close]
BLAST of CmaCh11G012350 vs. TAIR10
Match: AT5G56080.1 (AT5G56080.1 nicotianamine synthase 2)

HSP 1 Score: 333.2 bits (853), Expect = 1.8e-91
Identity = 174/325 (53.54%), Postives = 232/325 (71.38%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNP-L 60
           M  E   ++++++DLY QIS LESLKP K+VDTLF +LV TC+P T  N  +  + +  +
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLP-TDTNIDVTEIHDEKV 60

Query: 61  QQMRSNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRH 120
           + MRS+LI+LCG+AE  LE HFS +L +F D PL+HLH+FPY++NYLKL  LEF +L +H
Sbjct: 61  KDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQH 120

Query: 121 CPILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRM 180
              +P+ VAF+GSGP+PLTSIVLA  HLP+T+FHN+D+D  AN+ AS LVS DSDL  RM
Sbjct: 121 TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRM 180

Query: 181 VFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRA 240
           +FHT D++   E L +Y+VVFLAALVGM+KE K++ IEHL + MA GA +M+RSAHG RA
Sbjct: 181 IFHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRA 240

Query: 241 FLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVV 300
           FLYP+V+ CDL GFEVL+++HPSD+V+NSV+IARK          +G  N   G + G  
Sbjct: 241 FLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIARK----------LGGSNGARGSQIGRC 300

Query: 301 VHNKQCCDHIHGFNNHGGMIEELAE 324
           V     C  +H   N+ GM + L E
Sbjct: 301 VVMPCNCSKVHAILNNRGMEKNLIE 314

[Close]
BLAST of CmaCh11G012350 vs. TAIR10
Match: AT1G09240.1 (AT1G09240.1 nicotianamine synthase 3)

HSP 1 Score: 327.4 bits (838), Expect = 9.9e-90
Identity = 167/275 (60.73%), Postives = 216/275 (78.55%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           M  ++E L+Q + DLYE+IS LESLKP + V+ LF +LV TC+PP +PN  +  + + +Q
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPP-NPNIDVTKMCDRVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANF-DYPLHHLHLFPYFSNYLKLSLLEFSILRRHC 120
           ++R NLI++CG AE  LE+HFS +L ++ D PLHHL++FPY++NYLKL  LEF +L ++ 
Sbjct: 61  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 120

Query: 121 P-ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRM 180
              +P SVAF+GSGPLPLTSIVLA+ HL  T FHN+D+DP ANS AS LVS D D+  RM
Sbjct: 121 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 180

Query: 181 VFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRA 240
            FHT DIM VTE LK ++VVFLAALVGM KEEK++VIEHL + MA GA LM+RSAHG RA
Sbjct: 181 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 240

Query: 241 FLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARK 274
           FLYP+VE CDL GFEVLS++HP+D+VINSV+I++K
Sbjct: 241 FLYPIVEPCDLQGFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of CmaCh11G012350 vs. TAIR10
Match: AT5G04950.1 (AT5G04950.1 nicotianamine synthase 1)

HSP 1 Score: 316.6 bits (810), Expect = 1.7e-86
Identity = 155/276 (56.16%), Postives = 211/276 (76.45%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           M  +   ++++++DLY+QIS L+SLKP K+VDTLF +LV TC+P T  N  + ++   ++
Sbjct: 1   MACQNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLP-TDTNIDVTNMCEEVK 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDY---PLHHLHLFPYFSNYLKLSLLEFSILRR 120
            MR+NLI+LCG+AE  LE HFS +L +      PL HLH+FPY+SNYLKL  LEF +L +
Sbjct: 61  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 120

Query: 121 HCPILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTR 180
           H   +P+ +AFVGSGP+PLTSIVLA  HLP+T+FHN+D+D  AN+ AS LVS D DL  R
Sbjct: 121 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 180

Query: 181 MVFHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGR 240
           M+FHT D++  TE L +Y+VVFLAALVGM+KE K++ IEHL + MA GA LM+RSAH  R
Sbjct: 181 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 240

Query: 241 AFLYPVVEECDLVGFEVLSVFHPSDEVINSVIIARK 274
           AFLYP+V+  DL GF++L+++HP+D+V+NSV+IARK
Sbjct: 241 AFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of CmaCh11G012350 vs. TAIR10
Match: AT4G26483.1 (AT4G26483.1 nicotianamine synthases)

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-10
Identity = 32/54 (59.26%), Postives = 40/54 (74.07%), Query Frame = 1 

Query: 222 MAEGACLMVRSAHGGRAFLYPVVEECDLVGFEVLSVFHP--SDEVINSVIIARK 274
           M  GA LM+RSA G R+FLY  V+ CDL GFEVL ++HP  SD  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVARK 54

[Close]
BLAST of CmaCh11G012350 vs. NCBI nr
Match: gi|449435595|ref|XP_004135580.1| (PREDICTED: nicotianamine synthase-like [Cucumis sativus])

HSP 1 Score: 481.9 bits (1239), Expect = 8.9e-133
Identity = 253/336 (75.30%), Postives = 280/336 (83.33%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LIQKV +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVCEEETLIQKVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           QMRSNLIRLCGQAESLLE HFSDLLA FD P+ HLH+FPYFSNY+KLSLLEFSIL RH P
Sbjct: 61  QMRSNLIRLCGQAESLLEFHFSDLLAKFDSPIDHLHVFPYFSNYIKLSLLEFSILHRHGP 120

Query: 121 -ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180
             LPS+VAFVGSGPLPLTS+VLATRHL ST FHNYDLDP+ANS+AS LVS D DLKTRMV
Sbjct: 121 RALPSAVAFVGSGPLPLTSVVLATRHLTSTVFHNYDLDPVANSKASNLVSRDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEKEEKL+VI+HL E+M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKEEKLKVIKHLSEYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK-------------DDDCLKIVGD 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K             D+ C   V  
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTMKFDNDNDSDDNNNNDNGCCLFVNS 300

Query: 301 DNHENGIENGVVVHNKQCCDHIHGFNNHGGMIEELA 323
           D +EN   +  VVHN +C +  +GFNNHGG IEE A
Sbjct: 301 DENENVKISSGVVHNNKCSEIQNGFNNHGGKIEEFA 336

[Close]
BLAST of CmaCh11G012350 vs. NCBI nr
Match: gi|659099330|ref|XP_008450545.1| (PREDICTED: nicotianamine synthase-like [Cucumis melo])

HSP 1 Score: 477.6 bits (1228), Expect = 1.7e-131
Identity = 255/344 (74.13%), Postives = 287/344 (83.43%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LIQ+V +LY++IS LESLKPC +VDTLFT+LVVTCM PTSP+FHINSLS PLQ
Sbjct: 1   MVCEEETLIQRVCELYDEISSLESLKPCNNVDTLFTKLVVTCMSPTSPHFHINSLSKPLQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           QMRS+LI LCGQAESLLESHFSDLLA FD P+ HLH+FPYFSNYLKLSLLEFSILRRH P
Sbjct: 61  QMRSSLISLCGQAESLLESHFSDLLAKFDSPIDHLHVFPYFSNYLKLSLLEFSILRRHGP 120

Query: 121 -ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMV 180
            +LPS+VAFVGSGPLPLTSIVLATRHL ST FHNYDLDP+ANS+AS LVS+D DLKTRMV
Sbjct: 121 RVLPSAVAFVGSGPLPLTSIVLATRHLTSTVFHNYDLDPVANSKASNLVSLDPDLKTRMV 180

Query: 181 FHTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAF 240
           FHTCDIMKVTEELK+YEVVFLAALVGMEK+EKL+VI+HL ++M+EGA LMVRSAHGGRAF
Sbjct: 181 FHTCDIMKVTEELKQYEVVFLAALVGMEKDEKLKVIKHLSDYMSEGAYLMVRSAHGGRAF 240

Query: 241 LYPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKK-----------DDDCLKIVGDDN 300
           LYPVVE+ DL+GFEVLSVFHP+DEVINSVIIARK  K           DD+C   V  D 
Sbjct: 241 LYPVVEDSDLLGFEVLSVFHPTDEVINSVIIARKTTKYDNENDSNDNNDDNCCLFVNSDE 300

Query: 301 HE-----NGI----ENGVVVHNKQCCDHIHGF-NNHGGMIEELA 323
           +E     +GI    +N  VVHN +C +  +GF NNHGG IEE A
Sbjct: 301 NEKVKISSGIDHKKDNNGVVHNNKCSEIQNGFNNNHGGKIEEFA 344

[Close]
BLAST of CmaCh11G012350 vs. NCBI nr
Match: gi|743890000|ref|XP_011038922.1| (PREDICTED: nicotianamine synthase-like [Populus euphratica])

HSP 1 Score: 382.1 bits (980), Expect = 9.6e-103
Identity = 203/324 (62.65%), Postives = 253/324 (78.09%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV ++E L+QKV DLYEQIS LESLKP K VD LFT+LV+TC+PP      +N L   +Q
Sbjct: 1   MVCQKEPLVQKVCDLYEQISSLESLKPSKDVDMLFTQLVLTCIPPNP--IDVNKLCKKIQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           +MRS LIRLCG+AE LLESHFS +L +++ PL HL++FPY+SNYLKLS LEF+IL++HC 
Sbjct: 61  EMRSKLIRLCGEAEGLLESHFSAILGSYENPLDHLNVFPYYSNYLKLSHLEFNILKQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +PS VAFVGSGPLPLTSIVLA  HL +TSFHNYD+DP ANS+A +LV+   DL +RM F
Sbjct: 121 NVPSKVAFVGSGPLPLTSIVLACNHLTATSFHNYDIDPSANSKALQLVTSHPDLSSRMFF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DIM VT EL+E++VVFLAALVGM+KEEK++VI+HL + MA GA LM+RSAHG RAFL
Sbjct: 181 HTTDIMDVTSELQEFDVVFLAALVGMDKEEKVKVIDHLAKQMAPGAILMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPV++ CDL GFEVLSVFHPSDEVINSV+IARK       + +   D+ + G+ + +V+ 
Sbjct: 241 YPVIDPCDLRGFEVLSVFHPSDEVINSVVIARKYS-----MPLKSIDHQQTGLGSMIVLP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK CC+ I  FN  +H  M+EELA
Sbjct: 301 NK-CCE-ILAFNPLSHVTMVEELA 315

[Close]
BLAST of CmaCh11G012350 vs. NCBI nr
Match: gi|356572930|ref|XP_003554618.1| (PREDICTED: nicotianamine synthase-like [Glycine max])

HSP 1 Score: 380.2 bits (975), Expect = 3.7e-102
Identity = 202/324 (62.35%), Postives = 246/324 (75.93%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV  LYEQIS LESLKPCK+VD LFT+LV+TC+PP+     +  L+  +Q
Sbjct: 1   MVCEEELLIEKVCSLYEQISSLESLKPCKNVDMLFTKLVLTCIPPSP--IDVTKLNKTVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
            +RS+LI+LCG+AE  LESH+S +L ++D PL+HL++FPY+SNYLKL  LEF+IL +HC 
Sbjct: 61  DIRSHLIKLCGEAEGHLESHYSTILGSYDKPLNHLNIFPYYSNYLKLGHLEFTILSQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +P+ VAFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A +LVS D DL  RM F
Sbjct: 121 HVPTKVAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNAMRLVSSDPDLSKRMFF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI++V+  LKEYEVV+LAALVGM+KE K R+I+HL + M  GA LM+RSAHG RAFL
Sbjct: 181 HTNDILEVSNALKEYEVVYLAALVGMDKEGKNRIIDHLGKHMNPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPVVE CD  GFEVLSVFHPSDEVINSV+IARK              + E G+   +++ 
Sbjct: 241 YPVVEPCDFRGFEVLSVFHPSDEVINSVVIARKLY-----------HSLEQGLVGSMILP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK  C  I  FN  NHG MIEELA
Sbjct: 301 NK--CSEIQAFNPLNHGNMIEELA 309

[Close]
BLAST of CmaCh11G012350 vs. NCBI nr
Match: gi|1012360113|gb|KYP71297.1| (Nicotianamine synthase [Cajanus cajan])

HSP 1 Score: 379.0 bits (972), Expect = 8.1e-102
Identity = 201/324 (62.04%), Postives = 251/324 (77.47%), Query Frame = 1 

Query: 1   MVSEEEALIQKVVDLYEQISGLESLKPCKHVDTLFTELVVTCMPPTSPNFHINSLSNPLQ 60
           MV EEE LI+KV +LYEQIS LESLKP K+VD LFT+LV+TC+PP+     +  L   +Q
Sbjct: 1   MVCEEEMLIEKVCELYEQISSLESLKPSKNVDMLFTQLVLTCIPPSP--IDVTKLDKTVQ 60

Query: 61  QMRSNLIRLCGQAESLLESHFSDLLANFDYPLHHLHLFPYFSNYLKLSLLEFSILRRHCP 120
           ++RS+LIRLCG+AE  LE+H+S +L ++D PL HL++FPY+SNYLKL LLEF+IL +HC 
Sbjct: 61  EIRSHLIRLCGEAEGHLETHYSTILGSYDNPLDHLNIFPYYSNYLKLGLLEFTILSQHCT 120

Query: 121 ILPSSVAFVGSGPLPLTSIVLATRHLPSTSFHNYDLDPLANSQASKLVSVDSDLKTRMVF 180
            +P+ +AFVGSGPLPLTSIVLA+ HLPST+FHNYD+DPLANS A++LVS D DL  RMVF
Sbjct: 121 HVPTKIAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNATRLVSSDPDLSKRMVF 180

Query: 181 HTCDIMKVTEELKEYEVVFLAALVGMEKEEKLRVIEHLCEFMAEGACLMVRSAHGGRAFL 240
           HT DI+ V+  LKEYEVV+LAALVGM+KE K R+I+HL + MA GA LM+RSAHG RAFL
Sbjct: 181 HTTDILDVSNGLKEYEVVYLAALVGMDKEGKNRIIDHLAKNMAPGALLMLRSAHGARAFL 240

Query: 241 YPVVEECDLVGFEVLSVFHPSDEVINSVIIARKKKKDDDCLKIVGDDNHENGIENGVVVH 300
           YPVVE CDL GFEVLSVFHP+DEVINSV+IARK  +      +      + G+ + +++ 
Sbjct: 241 YPVVEPCDLRGFEVLSVFHPTDEVINSVVIARKYPQ-----PVPMHSLDQQGLAS-MILP 300

Query: 301 NKQCCDHIHGFN--NHGGMIEELA 323
           NK  C  I  FN  NHG +IEELA
Sbjct: 301 NK--CSEIQAFNPLNHGNIIEELA 314

The following BLAST results are available for this feature:
BLAST of CmaCh11G012350 vs. Swiss-Prot
Match NameE-valueIdentityDescription
NAS4_ARATH2.0e-9261.23Probable nicotianamine synthase 4 OS=Arabidopsis thaliana GN=NAS4 PE=2 SV=1[more]
NAS_SOLLC4.5e-9255.84Nicotianamine synthase OS=Solanum lycopersicum GN=CHLN PE=2 SV=1[more]
NAS2_ARATH3.2e-9053.54Nicotianamine synthase 2 OS=Arabidopsis thaliana GN=NAS2 PE=2 SV=1[more]
NAS3_ARATH1.8e-8860.73Nicotianamine synthase 3 OS=Arabidopsis thaliana GN=NAS3 PE=2 SV=1[more]
NAS1_ARATH3.1e-8556.16Nicotianamine synthase 1 OS=Arabidopsis thaliana GN=NAS1 PE=2 SV=1[more]
BLAST of CmaCh11G012350 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LVX1_CUCSA6.2e-13375.30Uncharacterized protein OS=Cucumis sativus GN=Csa_1G561410 PE=4 SV=1[more]
I1NBR5_SOYBN2.5e-10262.35Uncharacterized protein OS=Glycine max GN=GLYMA_19G228400 PE=4 SV=1[more]
A0A0B2SE88_GLYSO2.5e-10262.35Nicotianamine synthase OS=Glycine soja GN=glysoja_001928 PE=4 SV=1[more]
A0A151TW62_CAJCA5.7e-10262.04Nicotianamine synthase OS=Cajanus cajan GN=KK1_010551 PE=4 SV=1[more]
A0A0S3RZV4_PHAAN1.6e-10161.11Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G374000 PE=... [more]
BLAST of CmaCh11G012350 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G56430.11.1e-9361.23 nicotianamine synthase 4[more]
AT5G56080.11.8e-9153.54 nicotianamine synthase 2[more]
AT1G09240.19.9e-9060.73 nicotianamine synthase 3[more]
AT5G04950.11.7e-8656.16 nicotianamine synthase 1[more]
AT4G26483.12.7e-1059.26 nicotianamine synthases[more]
BLAST of CmaCh11G012350 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449435595|ref|XP_004135580.1|8.9e-13375.30PREDICTED: nicotianamine synthase-like [Cucumis sativus][more]
gi|659099330|ref|XP_008450545.1|1.7e-13174.13PREDICTED: nicotianamine synthase-like [Cucumis melo][more]
gi|743890000|ref|XP_011038922.1|9.6e-10362.65PREDICTED: nicotianamine synthase-like [Populus euphratica][more]
gi|356572930|ref|XP_003554618.1|3.7e-10262.35PREDICTED: nicotianamine synthase-like [Glycine max][more]
gi|1012360113|gb|KYP71297.1|8.1e-10262.04Nicotianamine synthase [Cajanus cajan][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004298Nicotian_synth
Vocabulary: Molecular Function
TermDefinition
GO:0030410nicotianamine synthase activity
Vocabulary: Biological Process
TermDefinition
GO:0030418nicotianamine biosynthetic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030410 nicotianamine synthase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G012350.1CmaCh11G012350.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 4..276
score: 3.4E
IPR004298Nicotianamine synthasePROFILEPS51142NAScoord: 1..278
score: 56
NoneNo IPR availablePANTHERPTHR32266FAMILY NOT NAMEDcoord: 1..274
score: 4.0E
NoneNo IPR availablePANTHERPTHR32266:SF12NICOTIANAMINE SYNTHASE-RELATEDcoord: 1..274
score: 4.0E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh11G012350CmoCh11G012910Cucurbita moschata (Rifu)cmacmoB115
CmaCh11G012350Lsi06G000550Bottle gourd (USVL1VR-Ls)cmalsiB142
CmaCh11G012350Cp4.1LG04g08620Cucurbita pepo (Zucchini)cmacpeB150
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh11G012350Cucurbita maxima (Rimu)cmacmaB085
CmaCh11G012350Cucumber (Gy14) v1cgycmaB0055
CmaCh11G012350Wild cucumber (PI 183967)cmacpiB105
CmaCh11G012350Cucumber (Chinese Long) v2cmacuB106
CmaCh11G012350Melon (DHL92) v3.5.1cmameB108
CmaCh11G012350Watermelon (Charleston Gray)cmawcgB114
CmaCh11G012350Bottle gourd (USVL1VR-Ls)cmalsiB133
CmaCh11G012350Melon (DHL92) v3.6.1cmamedB116
CmaCh11G012350Cucumber (Chinese Long) v3cmacucB0120
CmaCh11G012350Watermelon (97103) v2cmawmbB117
CmaCh11G012350Wax gourdcmawgoB0171