CmaCh11G011550 (gene) Cucurbita maxima (Rimu)

NameCmaCh11G011550
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSaccharopine dehydrogenase-like protein
LocationCma_Chr11 : 6686197 .. 6687641 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCTCCCCTTTACGATTTCATAATCCTAGCCGCCTCTGGTTTCACCGGCAAGTACGTGGTCCGACAAGCCCTCAAATTCCTCAACCCATCTCCTTCTTCCCCTCTCAAATCCTTCGCTCTAGCTGGTCGGAATCCGACAAAACTGTCGGAAACACTCAAATGGGCAGCTCATCCCAACTCCCCGCCGTCAATTCCGCTTCTAACCGCCGATATTTCCGACCCACAATCCATCCACCGCCTCTGCACCCAAACCAAGCTCATCCTCAACTGCGCCGGACCCTTCCGCCGCTACGGCGAGCCGGTGGTGGCGGCTTGTGTAGAGACAGGGTGCGATTATTTGGACATTTGTGGGGAGCCGGAGTTCATAGAGAAAATGGAAGCTAATTATCATGAGAGAGCGGTTGAGAATGGTTGTTTGGTGATTTCGGCTTGTGGGTTCGACTCGGTTCCGGCTGAATTGGGTTTTATTTTCAATTCCAAGCAGTGGGTTGGGGAATCGGCGCCGAACCGGATCGAGGCGTATTTGAGCTTGGAATCCAAGAACAAAATTGTTGCGAATTTTGGGACATTTGAGTCGGCTGTTTTGGGTGTCGCTAATGCCGACCGGTTAGTGCAATTGCGGCGGTCGAGGTCTAGAAGATCTCGGCCAACGGTGAGGAAAATTCCACAATTTAAAAATATATTTGAAAATATTTGAAATTTATGGAACATTATTAAAATTTATTATGAATTTTGTTGTGTCAGATTCCGGGTTGTCCTCCTCCTAAAGGACCAACTGTGGAACACAACAAAGAGACAAGCCTTTGGGCTGTAAGATTACCTTCAGCTGATTCTACTGTTGTTCGTAGAACACTCTCTTCTCTAGCTGAAAACCCTCATGGTCTTCCCGGTGTTAACGAGCGTGTCGAAGAGATCGAACAGAGGACGACGTTTTGGTCCTCTATGAAGCCAGCTCATTTCGGCGTAAAAATTGGCCATAAGTCATTGATTTGCATTCTCCAAATGATTGTAGTTGGAATCATCATAGGTTTGTTGAGTAGAAGTTCATTCGGGACGTGGCTTCTTTTGACGTTCCCTTCGATTTTTAGTCTCGGTTGGTTTAGGAAGAACGGTCCTTCTGAAGACGAGGTCGATAGTGCGTCGTTTAAGATGTGGTTTGTTGGTCATGGTTTTAGTAATAATAATGGTGGAGGGAGTCGAGAACTGGATATGAAAATTGTAACGAGAGTGATGGGGCCTGAATCTGGATATGTAGCTACTTCGATAATCCTTGTTCAATGTGCTTTGGTTGTTCTAAGCAAACGCGAGATTCTTCCGAAAGGAGGAGTTCTTACTCCTGGTATTGTGTTCGGACCTACTGATCTTCAGAAAAGACTCGAAGAGAATGAGATATGTTTTGATGTTATTTCAAAGCATGCTCTATTGCAGAAATTGCTGTAA

mRNA sequence

ATGGAGGCTCCCCTTTACGATTTCATAATCCTAGCCGCCTCTGGTTTCACCGGCAAGTACGTGGTCCGACAAGCCCTCAAATTCCTCAACCCATCTCCTTCTTCCCCTCTCAAATCCTTCGCTCTAGCTGGTCGGAATCCGACAAAACTGTCGGAAACACTCAAATGGGCAGCTCATCCCAACTCCCCGCCGTCAATTCCGCTTCTAACCGCCGATATTTCCGACCCACAATCCATCCACCGCCTCTGCACCCAAACCAAGCTCATCCTCAACTGCGCCGGACCCTTCCGCCGCTACGGCGAGCCGGTGGTGGCGGCTTGTGTAGAGACAGGGTGCGATTATTTGGACATTTGTGGGGAGCCGGAGTTCATAGAGAAAATGGAAGCTAATTATCATGAGAGAGCGGTTGAGAATGGTTGTTTGGTGATTTCGGCTTGTGGGTTCGACTCGGTTCCGGCTGAATTGGGTTTTATTTTCAATTCCAAGCAGTGGGTTGGGGAATCGGCGCCGAACCGGATCGAGGCGTATTTGAGCTTGGAATCCAAGAACAAAATTGTTGCGAATTTTGGGACATTTGAGTCGGCTGTTTTGGGTGTCGCTAATGCCGACCGGTTAGTGCAATTGCGGCGGTCGAGGTCTAGAAGATCTCGGCCAACGATTCCGGGTTGTCCTCCTCCTAAAGGACCAACTGTGGAACACAACAAAGAGACAAGCCTTTGGGCTGTAAGATTACCTTCAGCTGATTCTACTGTTGTTCGTAGAACACTCTCTTCTCTAGCTGAAAACCCTCATGGTCTTCCCGGTGTTAACGAGCGTGTCGAAGAGATCGAACAGAGGACGACGTTTTGGTCCTCTATGAAGCCAGCTCATTTCGGCGTAAAAATTGGCCATAAGTCATTGATTTGCATTCTCCAAATGATTGTAGTTGGAATCATCATAGGTTTGTTGAGTAGAAGTTCATTCGGGACGTGGCTTCTTTTGACGTTCCCTTCGATTTTTAGTCTCGGTTGGTTTAGGAAGAACGGTCCTTCTGAAGACGAGGTCGATAGTGCGTCGTTTAAGATGTGGTTTGTTGGTCATGGTTTTAGTAATAATAATGGTGGAGGGAGTCGAGAACTGGATATGAAAATTGTAACGAGAGTGATGGGGCCTGAATCTGGATATGTAGCTACTTCGATAATCCTTGTTCAATGTGCTTTGGTTGTTCTAAGCAAACGCGAGATTCTTCCGAAAGGAGGAGTTCTTACTCCTGGTATTGTGTTCGGACCTACTGATCTTCAGAAAAGACTCGAAGAGAATGAGATATGTTTTGATGTTATTTCAAAGCATGCTCTATTGCAGAAATTGCTGTAA

Coding sequence (CDS)

ATGGAGGCTCCCCTTTACGATTTCATAATCCTAGCCGCCTCTGGTTTCACCGGCAAGTACGTGGTCCGACAAGCCCTCAAATTCCTCAACCCATCTCCTTCTTCCCCTCTCAAATCCTTCGCTCTAGCTGGTCGGAATCCGACAAAACTGTCGGAAACACTCAAATGGGCAGCTCATCCCAACTCCCCGCCGTCAATTCCGCTTCTAACCGCCGATATTTCCGACCCACAATCCATCCACCGCCTCTGCACCCAAACCAAGCTCATCCTCAACTGCGCCGGACCCTTCCGCCGCTACGGCGAGCCGGTGGTGGCGGCTTGTGTAGAGACAGGGTGCGATTATTTGGACATTTGTGGGGAGCCGGAGTTCATAGAGAAAATGGAAGCTAATTATCATGAGAGAGCGGTTGAGAATGGTTGTTTGGTGATTTCGGCTTGTGGGTTCGACTCGGTTCCGGCTGAATTGGGTTTTATTTTCAATTCCAAGCAGTGGGTTGGGGAATCGGCGCCGAACCGGATCGAGGCGTATTTGAGCTTGGAATCCAAGAACAAAATTGTTGCGAATTTTGGGACATTTGAGTCGGCTGTTTTGGGTGTCGCTAATGCCGACCGGTTAGTGCAATTGCGGCGGTCGAGGTCTAGAAGATCTCGGCCAACGATTCCGGGTTGTCCTCCTCCTAAAGGACCAACTGTGGAACACAACAAAGAGACAAGCCTTTGGGCTGTAAGATTACCTTCAGCTGATTCTACTGTTGTTCGTAGAACACTCTCTTCTCTAGCTGAAAACCCTCATGGTCTTCCCGGTGTTAACGAGCGTGTCGAAGAGATCGAACAGAGGACGACGTTTTGGTCCTCTATGAAGCCAGCTCATTTCGGCGTAAAAATTGGCCATAAGTCATTGATTTGCATTCTCCAAATGATTGTAGTTGGAATCATCATAGGTTTGTTGAGTAGAAGTTCATTCGGGACGTGGCTTCTTTTGACGTTCCCTTCGATTTTTAGTCTCGGTTGGTTTAGGAAGAACGGTCCTTCTGAAGACGAGGTCGATAGTGCGTCGTTTAAGATGTGGTTTGTTGGTCATGGTTTTAGTAATAATAATGGTGGAGGGAGTCGAGAACTGGATATGAAAATTGTAACGAGAGTGATGGGGCCTGAATCTGGATATGTAGCTACTTCGATAATCCTTGTTCAATGTGCTTTGGTTGTTCTAAGCAAACGCGAGATTCTTCCGAAAGGAGGAGTTCTTACTCCTGGTATTGTGTTCGGACCTACTGATCTTCAGAAAAGACTCGAAGAGAATGAGATATGTTTTGATGTTATTTCAAAGCATGCTCTATTGCAGAAATTGCTGTAA

Protein sequence

MEAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEPEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLESKNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWAVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHGFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIVFGPTDLQKRLEENEICFDVISKHALLQKLL
BLAST of CmaCh11G011550 vs. Swiss-Prot
Match: SCPDL_ARATH (Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 OS=Arabidopsis thaliana GN=At5g39410 PE=1 SV=2)

HSP 1 Score: 615.1 bits (1585), Expect = 5.9e-175
Identity = 293/442 (66.29%), Postives = 361/442 (81.67%), Query Frame = 1

Query: 4   PLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNSP 63
           P+YD +IL ASGFTGKYVVR+ALKFL    SSPLKS ALAGRNPT+L+++L+WAA PN P
Sbjct: 9   PVYDMVILGASGFTGKYVVREALKFLQTPSSSPLKSLALAGRNPTRLTQSLEWAARPNPP 68

Query: 64  PS-IPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEPE 123
           PS + +LTAD SDP S+ RLCTQTKLILNC GPFR +G+PVV+AC ++GCDYLDI GEPE
Sbjct: 69  PSSVAILTADTSDPDSLRRLCTQTKLILNCVGPFRIHGDPVVSACADSGCDYLDISGEPE 128

Query: 124 FIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLESK 183
           F+E+MEANYH+RA E G L++SACGFDS+PAELG +FN+KQWV  S PN+IEAYLSLES 
Sbjct: 129 FMERMEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLESD 188

Query: 184 NKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWAV 243
            KI  NFGT+ESAVLGVANA++L +LRRSR RR RPTI G PP KGPT+E+ K   LWA+
Sbjct: 189 KKIAGNFGTYESAVLGVANAEKLKELRRSRPRRPRPTICG-PPAKGPTLENQKTIGLWAL 248

Query: 244 RLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLIC 303
           +LPSAD+ VVRRTL++L E PHGLPG+NE  E+I++R  FWSS+KPAHFGVKI  KSL  
Sbjct: 249 KLPSADAVVVRRTLTTLTEKPHGLPGINESPEQIQKREAFWSSIKPAHFGVKITSKSLFG 308

Query: 304 ILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHGF 363
           I + + +G+ +GLLS+ SFG WLLL FPS+FSLGWF+K GPSE+EV+SA+FKMWF+G G+
Sbjct: 309 IFRYVTLGVSLGLLSKFSFGRWLLLKFPSVFSLGWFQKKGPSEEEVESATFKMWFIGRGY 368

Query: 364 SNNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 423
           S  +    G  + D++I+TR+ GPE GY+ T I LVQC L+VL +RE L KGGV TPGIV
Sbjct: 369 SEESLASQGETKPDLEIITRISGPEIGYITTPITLVQCGLIVLGQRESLVKGGVYTPGIV 428

Query: 424 FGPTDLQKRLEENEICFDVISK 443
           FG TD+Q+RLE+N I F++ISK
Sbjct: 429 FGSTDIQQRLEDNGISFELISK 449

BLAST of CmaCh11G011550 vs. Swiss-Prot
Match: SCPDL_MOUSE (Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.8e-68
Identity = 153/442 (34.62%), Postives = 241/442 (54.52%), Query Frame = 1

Query: 6   YDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNSPPS 65
           +  ++  ASGFTG++V  +  +    S  S    +A+AGR+  KL + L+ AA     PS
Sbjct: 8   FHLVVFGASGFTGQFVTEEVAREQIASEQSSRLPWAVAGRSKEKLQQVLEKAAQKLGRPS 67

Query: 66  IP----LLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 125
           +     ++  DIS+P S+  +  Q KL+LNC GP+R YGEPVV AC+E G   +DICGEP
Sbjct: 68  LSSEVGVIICDISNPASLDEMAKQAKLVLNCVGPYRFYGEPVVKACIENGTSCIDICGEP 127

Query: 126 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 185
           +F+E M A YHE+A E G  +I + GFDS+PA+LG ++   Q  G      +E++L++ +
Sbjct: 128 QFLELMHAKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNGTLTA--VESFLTINT 187

Query: 186 KNK-IVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 245
             + +  + GT++SA+ G  +   L +LR     +  P + G    +   V + +E + +
Sbjct: 188 GPEGLCIHDGTWKSAIYGFGDKGSLRKLRSVSCLKPVPIV-GTKLKRRWPVSYCRELNSY 247

Query: 246 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 305
           ++    +D +VV+RT   L EN          +E+            P  +   +    +
Sbjct: 248 SIPFLGSDISVVKRTQRYLHEN----------LED-----------SPVQYAAYVTVGGI 307

Query: 306 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 365
             +++++  G+      + S G  LL+ FP +FS G+F K GP++ ++D  SF M F G 
Sbjct: 308 TSVIKLMFAGLFFLFFVKFSIGRQLLIKFPWLFSFGYFSKQGPTQKQMDETSFTMTFFGQ 367

Query: 366 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPK-GGVLTPGI 425
           G+S+       + +++I T+V GPE+GYVAT I +VQ A+  LS    LPK GGV TPG 
Sbjct: 368 GYSHGTCVEKNKPNIRICTQVKGPEAGYVATPIAMVQAAMTFLSDASDLPKGGGVFTPGA 425

Query: 426 VFGPTDLQKRLEENEICFDVIS 442
            F  T L  RL ++ I F VIS
Sbjct: 428 AFSRTKLIDRLNKHGIEFSVIS 425

BLAST of CmaCh11G011550 vs. Swiss-Prot
Match: SCPDL_RAT (Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus GN=Sccpdh PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.4e-67
Identity = 151/442 (34.16%), Postives = 238/442 (53.85%), Query Frame = 1

Query: 6   YDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP----N 65
           +  ++  ASGFTG++V  +  +    S  S    +A+AGR+  KL + L+ AA       
Sbjct: 8   FQLVVFGASGFTGQFVTEEVAREQMASEQSSRLPWAVAGRSKEKLQQVLEKAAQKLGRAT 67

Query: 66  SPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 125
               + ++  DIS+P S+  +  +  L+LNC GP+R YGEPVV AC+E G   +DICGEP
Sbjct: 68  LSSEVGIIICDISNPASLDEMAKKATLVLNCVGPYRFYGEPVVKACIENGTSCIDICGEP 127

Query: 126 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 185
           +F+E M   YHE+A E G  +I + GFDS+PA+LG ++   Q  G      +E++L++ S
Sbjct: 128 QFLELMHVKYHEKAAEKGVYIIGSSGFDSIPADLGVLYTRNQMNG--TLTAVESFLTINS 187

Query: 186 -KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 245
               +  + GT++SA+ G  +   L +LR   + +  P I      + P V + +E + +
Sbjct: 188 GPEGLCIHDGTWKSAIYGFGDKGSLRKLRSVSNLKPVPVIGSKLKRRWP-VSYCRELNSY 247

Query: 246 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 305
           A+    +D +VV+RT   L EN          +E+            P  +   I    +
Sbjct: 248 AIPFLGSDMSVVKRTQRYLHEN----------LED-----------SPVQYAAYITVGGI 307

Query: 306 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 365
             +++++  G+      + S G  LL+ FP +FS G+F K GP++ ++D +SF M F G 
Sbjct: 308 TSVIKLMFAGLFFLFFVKFSIGRQLLVKFPWLFSFGYFSKRGPTQKQMDESSFTMTFFGQ 367

Query: 366 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPK-GGVLTPGI 425
           G+S+       + +++I T+V GPE+GYVAT I +VQ A+  L+    LPK GGV TPG 
Sbjct: 368 GYSHGVSAEKNKPNIRICTQVKGPEAGYVATPIAMVQAAVTFLNDASDLPKGGGVFTPGA 425

Query: 426 VFGPTDLQKRLEENEICFDVIS 442
            F  T L  RL ++ I F VIS
Sbjct: 428 AFSRTKLIDRLNQHGIQFSVIS 425

BLAST of CmaCh11G011550 vs. Swiss-Prot
Match: SCPDL_HUMAN (Saccharopine dehydrogenase-like oxidoreductase OS=Homo sapiens GN=SCCPDH PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.1e-67
Identity = 153/443 (34.54%), Postives = 242/443 (54.63%), Query Frame = 1

Query: 6   YDFIILAASGFTGKYVVRQ-ALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAA----HP 65
           +  ++  ASGFTG++V  + A + ++P  SS L  +A+AGR+  KL   L+ AA     P
Sbjct: 8   FHLVVFGASGFTGQFVTEEVAREQVDPERSSRLP-WAVAGRSREKLQRVLEKAALKLGRP 67

Query: 66  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 125
                + ++  DI++P S+  +  Q  ++LNC GP+R YGEPV+ AC+E G   +DI GE
Sbjct: 68  TLSSEVGIIICDIANPASLDEMAKQATVVLNCVGPYRFYGEPVIKACIENGASCIDISGE 127

Query: 126 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 185
           P+F+E M+  YHE+A + G  +I + GFDS+PA+LG I+   +  G      +E++L++ 
Sbjct: 128 PQFLELMQLKYHEKAADKGVYIIGSSGFDSIPADLGVIYTRNKMNG--TLTAVESFLTIH 187

Query: 186 S-KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSL 245
           S    +  + GT++SA+ G  +   L +LR   + +  P I      + P + + +E   
Sbjct: 188 SGPEGLSIHDGTWKSAIYGFGDQSNLRKLRNVSNLKPVPLIGPKLKRRWP-ISYCRELKG 247

Query: 246 WAVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKS 305
           +++    +D +VVRRT   L EN          +EE            P  +   +    
Sbjct: 248 YSIPFMGSDVSVVRRTQRYLYEN----------LEE-----------SPVQYAAYVTVGG 307

Query: 306 LICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVG 365
           +  +++++  G+      R   G  LL+ FP  FS G+F K GP++ ++D+ASF + F G
Sbjct: 308 ITSVIKLMFAGLFFLFFVRFGIGRQLLIKFPWFFSFGYFSKQGPTQKQIDAASFTLTFFG 367

Query: 366 HGFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPK-GGVLTPG 425
            G+S   G    + ++KI T+V GPE+GYVAT I +VQ A+ +LS    LPK GGV TPG
Sbjct: 368 QGYSQGTGTDKNKPNIKICTQVKGPEAGYVATPIAMVQAAMTLLSDASHLPKAGGVFTPG 425

Query: 426 IVFGPTDLQKRLEENEICFDVIS 442
             F  T L  RL ++ I F VIS
Sbjct: 428 AAFSKTKLIDRLNKHGIEFSVIS 425

BLAST of CmaCh11G011550 vs. Swiss-Prot
Match: SCPDL_BOVIN (Saccharopine dehydrogenase-like oxidoreductase OS=Bos taurus GN=SCCPDH PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.4e-66
Identity = 152/443 (34.31%), Postives = 242/443 (54.63%), Query Frame = 1

Query: 6   YDFIILAASGFTGKYVVRQ-ALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAA----HP 65
           +  ++  ASGFTG++V  + A + ++P  +S L  +A+AGR+  KL   L+ AA     P
Sbjct: 8   FHLVVFGASGFTGQFVTEEVAREQVSPERTSHLP-WAVAGRSREKLLRVLERAAMKLGRP 67

Query: 66  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 125
                + ++  DI++P S+  +  Q  ++LNC GP+R YGEPV+ AC+E G   +DI GE
Sbjct: 68  TLSSEVGIIICDITNPASLDEMAKQATVVLNCVGPYRFYGEPVIKACIENGTSCIDISGE 127

Query: 126 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 185
           P+F+E M   YHE+A E G  +I + GFDS+PA+LG I+   +  G      +E++L++ 
Sbjct: 128 PQFLELMYWKYHEKAAEKGVYIIGSSGFDSIPADLGVIYTRNKMNG--TLTAVESFLTIS 187

Query: 186 S-KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSL 245
           S    +  + GT++SAV G  +   L +LR     +  P +      + P + + +E + 
Sbjct: 188 SGPEGLCVHDGTWKSAVYGFGDKSNLKKLRNESDMKPVPIVGPKLKRRWP-ISYCRELNS 247

Query: 246 WAVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKS 305
           +++    AD +VV+RT   L EN             +EQ         P  +   I    
Sbjct: 248 YSIPFLGADVSVVKRTQRYLHEN-------------LEQ--------SPVQYAAYINVGG 307

Query: 306 LICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVG 365
           +  +++++  G+      R   G  LL+ F  +FS G+F K GP++ ++D++SF M F G
Sbjct: 308 ITSVIKLMFAGLFFLFFVRFGIGRQLLIKFTWLFSFGYFSKQGPTQKQIDASSFTMTFFG 367

Query: 366 HGFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPK-GGVLTPG 425
            GFS        + +++I T+V GPE+GYV+TSI +VQ A+++L+    LPK GGV TPG
Sbjct: 368 QGFSQGVSPVKNKPNIRICTQVKGPEAGYVSTSIAMVQAAMILLNDASDLPKAGGVFTPG 425

Query: 426 IVFGPTDLQKRLEENEICFDVIS 442
             F  T L  RL E+ I F VIS
Sbjct: 428 AAFSRTKLIDRLNEHGIEFSVIS 425

BLAST of CmaCh11G011550 vs. TrEMBL
Match: A0A0A0LF70_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824740 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.3e-205
Identity = 352/444 (79.28%), Postives = 395/444 (88.96%), Query Frame = 1

Query: 1   MEAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP 60
           ME   YD IIL A+GFTGKYVVR+AL+FLNPS  SPLKSFALAGRN TKL++TL+WAAHP
Sbjct: 34  MEGTPYDLIILGATGFTGKYVVREALRFLNPS--SPLKSFALAGRNLTKLTQTLQWAAHP 93

Query: 61  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 120
           +SPP IPLL ADI+DPQSIHRLCTQTKLILNC GPFRRYGEPVVAACVETGCDYLDICGE
Sbjct: 94  HSPPPIPLLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 153

Query: 121 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 180
           PEF+EKMEANYHERAV++G LV+SACGFDSVPAELG +FNS+QWVG++APNRIEAYLSLE
Sbjct: 154 PEFMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQTAPNRIEAYLSLE 213

Query: 181 SKNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 240
           S  KIV NFGTFESAVLGVANAD+L++LRRSR R+ RP IPG PPPKGPT+EH KE  LW
Sbjct: 214 SSKKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPKIPGPPPPKGPTIEHKKEIGLW 273

Query: 241 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 300
           +VRLPSADSTVVRRTLS+L ENP GLPGVNE   EIEQR TFWSS+KPAHFGVKIG KSL
Sbjct: 274 SVRLPSADSTVVRRTLSTLVENPQGLPGVNESAYEIEQRKTFWSSVKPAHFGVKIGTKSL 333

Query: 301 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 360
           I IL++I VG+ IGLL ++S G WLLLTFPS+FSLGWFRK GPSE+EV+SASFKMWFVGH
Sbjct: 334 IGILRIIAVGMFIGLLGKTSLGRWLLLTFPSVFSLGWFRKKGPSEEEVNSASFKMWFVGH 393

Query: 361 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 420
           GF ++N   + E +M+IVTRVMGPE GY+ T IILVQCAL+VLS+RE LPKGGVLTPGIV
Sbjct: 394 GFRSSNNEANVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSRREALPKGGVLTPGIV 453

Query: 421 FGPTDLQKRLEENEICFDVISKHA 445
           FGPTDLQ+RL+EN I FDVISK+A
Sbjct: 454 FGPTDLQQRLQENGISFDVISKNA 475

BLAST of CmaCh11G011550 vs. TrEMBL
Match: M5X4V6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005544mg PE=4 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.3e-186
Identity = 320/445 (71.91%), Postives = 370/445 (83.15%), Query Frame = 1

Query: 3   APLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNS 62
           +P YD IIL ASGFTGKYVV++ALKFLN +PSSPLKS ALAGR+PTKL++TLKWAAHPN 
Sbjct: 10  SPPYDLIILGASGFTGKYVVKEALKFLN-TPSSPLKSLALAGRSPTKLTQTLKWAAHPNP 69

Query: 63  PPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEPE 122
           PP IP++ AD +DP S+HRLCTQTKLILNC GPFR YGE VVAAC ETGCDYLDICGEPE
Sbjct: 70  PPPIPIINADTTDPPSLHRLCTQTKLILNCVGPFRLYGESVVAACAETGCDYLDICGEPE 129

Query: 123 FIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLESK 182
           F+E+MEA YH+RAVE G LVISACGFDSVPAE G +FNS+QWV  + PNR+EAY+SLES+
Sbjct: 130 FMERMEAKYHDRAVEVGSLVISACGFDSVPAEFGLMFNSRQWVDPALPNRVEAYVSLESE 189

Query: 183 NKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWAV 242
            +IV NFGTFESAVL VANAD+L +LRRSR R+ RP IPG PPPKGPT+E  K+  LWAV
Sbjct: 190 KRIVGNFGTFESAVLSVANADKLQELRRSRPRKPRPAIPGPPPPKGPTIEQQKDIGLWAV 249

Query: 243 RLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLIC 302
           +LPSAD+ VVRRTL  L ENP GLPG NE  E IE+R  FWS++KP HFGVKIG KSL+ 
Sbjct: 250 KLPSADAIVVRRTLGVLTENPRGLPGSNESAERIEKREAFWSTVKPVHFGVKIGSKSLLG 309

Query: 303 ILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHGF 362
           IL ++ VGI IGLL R SFG WLLL FPS FS+GWFRK GPSEDEV SASFKMWFVG GF
Sbjct: 310 ILHIMTVGIFIGLLGRFSFGRWLLLKFPSFFSVGWFRKKGPSEDEVRSASFKMWFVGKGF 369

Query: 363 SNNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 422
           S+++    G+R+ DM+I+TRVMGPE GY+ T IIL+QCAL++L +R  LPKGGV  PGIV
Sbjct: 370 SDSSLVSQGNRKPDMEIITRVMGPEIGYLTTPIILLQCALILLHQRNNLPKGGVFPPGIV 429

Query: 423 FGPTDLQKRLEENEICFDVISKHAL 446
           FGPTDLQ+RL+EN I FDVISK A+
Sbjct: 430 FGPTDLQERLQENGISFDVISKKAI 453

BLAST of CmaCh11G011550 vs. TrEMBL
Match: A0A067JTH7_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19896 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.0e-185
Identity = 318/446 (71.30%), Postives = 371/446 (83.18%), Query Frame = 1

Query: 2   EAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPN 61
           ++P YD IIL ASGFTGKYVVR+ALKFLN SPSSPLK+ ALAGRNP KLS+ L WAAHP 
Sbjct: 9   QSPPYDLIILGASGFTGKYVVREALKFLN-SPSSPLKTLALAGRNPAKLSQALNWAAHPK 68

Query: 62  SPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 121
           SPP IP+LTAD ++P SI  LCTQTKLILNC GPFR YG+PVVAAC ETGCDYLDICGEP
Sbjct: 69  SPPQIPILTADTTNPASIRHLCTQTKLILNCVGPFRLYGDPVVAACAETGCDYLDICGEP 128

Query: 122 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 181
           EF+E+ME  YHE+A+E G LV+SACG+DSVPAELG+IFNS+QWV  + PN+IEAYLSLES
Sbjct: 129 EFMERMEVKYHEKAMETGSLVVSACGYDSVPAELGWIFNSRQWVAPAVPNQIEAYLSLES 188

Query: 182 KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWA 241
           + KIV NFGT+ESAVLGVANAD+L +LRRSR +R+RPTIPG  PPKGPT+EH KE  LWA
Sbjct: 189 EKKIVGNFGTYESAVLGVANADKLQELRRSRPKRARPTIPGPFPPKGPTIEHQKELGLWA 248

Query: 242 VRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLI 301
           V+LPSADS VVRRTL++L ENPHGLPGVNE  E+IE+R  FWS +KPAHFGVK+G K++ 
Sbjct: 249 VKLPSADSVVVRRTLATLTENPHGLPGVNESAEQIEKREAFWSMVKPAHFGVKLGAKTVF 308

Query: 302 CILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHG 361
            + + I VGI IGLL R++ G WLLL FPS+FSLGWF+K GPSED+V SA+FKMWFVG G
Sbjct: 309 GVFRYITVGIFIGLLGRNAIGRWLLLKFPSVFSLGWFKKKGPSEDQVRSATFKMWFVGRG 368

Query: 362 FSNNNG--GGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGI 421
           FS+ N     + + DM+IVTRV GPE GY+ T IILVQCAL++LS+R  LPKGGVL PGI
Sbjct: 369 FSDINQVLQTTTKPDMEIVTRVTGPEIGYLTTPIILVQCALILLSERNTLPKGGVLPPGI 428

Query: 422 VFGPTDLQKRLEENEICFDVISKHAL 446
           VFGPTDLQ+RL  N I FD+ISK  L
Sbjct: 429 VFGPTDLQERLLLNGISFDIISKKTL 453

BLAST of CmaCh11G011550 vs. TrEMBL
Match: W9QQU6_9ROSA (Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Morus notabilis GN=L484_020963 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.4e-183
Identity = 311/446 (69.73%), Postives = 372/446 (83.41%), Query Frame = 1

Query: 2   EAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPN 61
           + P YD +IL ASGFTGKYVVR+ALKFLN +PSSPLKS ALAGRN TKL+E LKWAAHPN
Sbjct: 12  DLPRYDIVILGASGFTGKYVVREALKFLN-TPSSPLKSLALAGRNSTKLAEALKWAAHPN 71

Query: 62  SPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 121
           SPP IP+LTA+ SD  S+ RLC QT+L+LNC GPFR YGEPVVAACVETG DYLDI GEP
Sbjct: 72  SPPPIPILTAETSDAASLRRLCAQTRLVLNCVGPFRLYGEPVVAACVETGTDYLDISGEP 131

Query: 122 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 181
           EF+E+MEA +H++AVE   L +SACGFDSVPAE+G +FNS+QW G + PNR+EAYLSLES
Sbjct: 132 EFMERMEAKFHDKAVETASLAVSACGFDSVPAEIGLLFNSRQWTGPAVPNRVEAYLSLES 191

Query: 182 KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWA 241
             KIV NFGT+ESAVLG ANA +L++LRRSR +R+RP IPG PPPKGPT+EH KE +LWA
Sbjct: 192 DKKIVGNFGTYESAVLGFANAQKLMELRRSRPKRARPVIPGPPPPKGPTIEHQKEINLWA 251

Query: 242 VRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLI 301
           VRLPSADS VVRRTL++L ENP GLPG NE  E+I++R  FWS++KPA+FGVKIG KSL+
Sbjct: 252 VRLPSADSIVVRRTLATLTENPGGLPGKNESPEQIQKREAFWSTVKPAYFGVKIGFKSLL 311

Query: 302 CILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHG 361
            I +++ VGI IG+  R +FG WLLL FPS F+LGWFRK GPSE+EV  ASFKMWFVGHG
Sbjct: 312 GIFRIMTVGIFIGIFGRLAFGRWLLLKFPSFFTLGWFRKKGPSEEEVRRASFKMWFVGHG 371

Query: 362 FSNNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGI 421
           FS+++    G  + DM+IVTRVMGPE GY+ T IIL+QCAL+VLS+RE LPKGGV TPGI
Sbjct: 372 FSDSSLLSQGKSKPDMEIVTRVMGPEIGYLTTPIILLQCALIVLSQRENLPKGGVFTPGI 431

Query: 422 VFGPTDLQKRLEENEICFDVISKHAL 446
           VFGPT LQ+RL+EN + FD++SK+A+
Sbjct: 432 VFGPTYLQERLQENGVSFDLVSKNAI 456

BLAST of CmaCh11G011550 vs. TrEMBL
Match: B9H0F4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s12470g PE=4 SV=2)

HSP 1 Score: 649.0 bits (1673), Expect = 4.1e-183
Identity = 309/446 (69.28%), Postives = 374/446 (83.86%), Query Frame = 1

Query: 2   EAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPN 61
           + PLYD IIL ASGFTGKYVV++ALKFLN  PSSPLKS ALAGRNPTKL++TLKWA+HP+
Sbjct: 13  QPPLYDLIILGASGFTGKYVVKEALKFLNV-PSSPLKSLALAGRNPTKLAQTLKWASHPD 72

Query: 62  SPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 121
            PP IP+LTAD +DP S+H LC+Q+KLILNC GPFR  GEPVVAAC ETGCDYLDICGEP
Sbjct: 73  HPPPIPILTADTTDPASLHHLCSQSKLILNCVGPFRLLGEPVVAACAETGCDYLDICGEP 132

Query: 122 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 181
           EF+E+ME  YHE+A+E G LV+SACGFDSVPAELG++FNS+ WVG +APN+IEAYLSLES
Sbjct: 133 EFMERMEVKYHEKAMETGSLVVSACGFDSVPAELGWMFNSRHWVGPAAPNQIEAYLSLES 192

Query: 182 KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWA 241
           + +IV NFGT+ESAVLGVAN ++LV+LRRSR +R+RP IPG  P KGP ++H KE  LWA
Sbjct: 193 EKRIVGNFGTYESAVLGVANVEQLVELRRSRPKRARPAIPGPFPTKGPMIDHQKEIGLWA 252

Query: 242 VRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLI 301
           V+LPSADS VVRRTL++L ENP GLPG+NE  E+IE+R  FWS++KPAHFGVK+G K+L+
Sbjct: 253 VKLPSADSVVVRRTLTTLTENPRGLPGLNESPEQIEKRDAFWSTVKPAHFGVKLGSKTLL 312

Query: 302 CILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHG 361
            + + I VG+ IGLL R++ G WLLL FPS FSLGWFRK GPS+DEV SASFKMWFVG G
Sbjct: 313 GVFRFIAVGMFIGLLGRNAIGRWLLLKFPSFFSLGWFRKKGPSDDEVRSASFKMWFVGRG 372

Query: 362 FSNNN-GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 421
           FS+ N     ++ DM+I+TRV+GPE GY+ T IILVQCA ++LS R+ LPKGGV  PGIV
Sbjct: 373 FSDMNVSQDKKKPDMEIITRVVGPEIGYLTTPIILVQCARILLSHRDNLPKGGVFPPGIV 432

Query: 422 FGPTDLQKRLEENEICFDVISKHALL 447
           FGPTDLQ++LE+N I FD+ISK ++L
Sbjct: 433 FGPTDLQEQLEQNGISFDLISKKSIL 457

BLAST of CmaCh11G011550 vs. TAIR10
Match: AT5G39410.1 (AT5G39410.1 Saccharopine dehydrogenase )

HSP 1 Score: 615.1 bits (1585), Expect = 3.3e-176
Identity = 293/442 (66.29%), Postives = 361/442 (81.67%), Query Frame = 1

Query: 4   PLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNSP 63
           P+YD +IL ASGFTGKYVVR+ALKFL    SSPLKS ALAGRNPT+L+++L+WAA PN P
Sbjct: 9   PVYDMVILGASGFTGKYVVREALKFLQTPSSSPLKSLALAGRNPTRLTQSLEWAARPNPP 68

Query: 64  PS-IPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEPE 123
           PS + +LTAD SDP S+ RLCTQTKLILNC GPFR +G+PVV+AC ++GCDYLDI GEPE
Sbjct: 69  PSSVAILTADTSDPDSLRRLCTQTKLILNCVGPFRIHGDPVVSACADSGCDYLDISGEPE 128

Query: 124 FIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLESK 183
           F+E+MEANYH+RA E G L++SACGFDS+PAELG +FN+KQWV  S PN+IEAYLSLES 
Sbjct: 129 FMERMEANYHDRAEETGSLIVSACGFDSIPAELGLLFNAKQWVSPSVPNQIEAYLSLESD 188

Query: 184 NKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWAV 243
            KI  NFGT+ESAVLGVANA++L +LRRSR RR RPTI G PP KGPT+E+ K   LWA+
Sbjct: 189 KKIAGNFGTYESAVLGVANAEKLKELRRSRPRRPRPTICG-PPAKGPTLENQKTIGLWAL 248

Query: 244 RLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLIC 303
           +LPSAD+ VVRRTL++L E PHGLPG+NE  E+I++R  FWSS+KPAHFGVKI  KSL  
Sbjct: 249 KLPSADAVVVRRTLTTLTEKPHGLPGINESPEQIQKREAFWSSIKPAHFGVKITSKSLFG 308

Query: 304 ILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHGF 363
           I + + +G+ +GLLS+ SFG WLLL FPS+FSLGWF+K GPSE+EV+SA+FKMWF+G G+
Sbjct: 309 IFRYVTLGVSLGLLSKFSFGRWLLLKFPSVFSLGWFQKKGPSEEEVESATFKMWFIGRGY 368

Query: 364 SNNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 423
           S  +    G  + D++I+TR+ GPE GY+ T I LVQC L+VL +RE L KGGV TPGIV
Sbjct: 369 SEESLASQGETKPDLEIITRISGPEIGYITTPITLVQCGLIVLGQRESLVKGGVYTPGIV 428

Query: 424 FGPTDLQKRLEENEICFDVISK 443
           FG TD+Q+RLE+N I F++ISK
Sbjct: 429 FGSTDIQQRLEDNGISFELISK 449

BLAST of CmaCh11G011550 vs. NCBI nr
Match: gi|659085331|ref|XP_008443363.1| (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucumis melo])

HSP 1 Score: 736.5 bits (1900), Expect = 2.8e-209
Identity = 357/444 (80.41%), Postives = 399/444 (89.86%), Query Frame = 1

Query: 1   MEAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP 60
           ME P YD IIL A+GFTGKYVVR+ALKFLNPS  SPLKSFALAGRN TKL++TL+WAAHP
Sbjct: 1   MEGPPYDLIILGATGFTGKYVVREALKFLNPS--SPLKSFALAGRNLTKLTQTLQWAAHP 60

Query: 61  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 120
           +SPP IP L ADI+DPQSIHRLCTQTKLILNC GPFRRYGEPVVAACVETGCDYLDICGE
Sbjct: 61  HSPPPIPFLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 120

Query: 121 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 180
           PEF+EKMEANYHERAV++G LV+SACGFDSVPAELG +FNS+QWVG+ APNRIEAYLSLE
Sbjct: 121 PEFMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQMAPNRIEAYLSLE 180

Query: 181 SKNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 240
           SK KIV NFGTFESAVLGVANAD+L++LRRSR R+ RP IPG PPPKGPT+EH KE  LW
Sbjct: 181 SKKKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPQIPGPPPPKGPTIEHKKEIGLW 240

Query: 241 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 300
           AVRLPSADSTVVRRTLS+LAENP GLPGVNE  +EIEQR TFWSS+KPAHFGVKIG KSL
Sbjct: 241 AVRLPSADSTVVRRTLSTLAENPQGLPGVNESADEIEQRKTFWSSVKPAHFGVKIGTKSL 300

Query: 301 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 360
           I IL++I VG+ IGLL ++SFG WLLLTFPS+FSLGWFRK GPSE+EV+SASFKMWF+GH
Sbjct: 301 IGILRIITVGMFIGLLGKTSFGRWLLLTFPSVFSLGWFRKKGPSEEEVNSASFKMWFIGH 360

Query: 361 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 420
           GF ++N  G+ E +M+IVTRVMGPE GY+ T IILVQCAL+VLSKRE LPKGGVLTPGIV
Sbjct: 361 GFRSSNNEGNVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSKRESLPKGGVLTPGIV 420

Query: 421 FGPTDLQKRLEENEICFDVISKHA 445
           FGPTDLQ+RL+EN I FDV+SK+A
Sbjct: 421 FGPTDLQQRLQENGISFDVVSKNA 442

BLAST of CmaCh11G011550 vs. NCBI nr
Match: gi|449437814|ref|XP_004136685.1| (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 1.9e-205
Identity = 352/444 (79.28%), Postives = 395/444 (88.96%), Query Frame = 1

Query: 1   MEAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP 60
           ME   YD IIL A+GFTGKYVVR+AL+FLNPS  SPLKSFALAGRN TKL++TL+WAAHP
Sbjct: 1   MEGTPYDLIILGATGFTGKYVVREALRFLNPS--SPLKSFALAGRNLTKLTQTLQWAAHP 60

Query: 61  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 120
           +SPP IPLL ADI+DPQSIHRLCTQTKLILNC GPFRRYGEPVVAACVETGCDYLDICGE
Sbjct: 61  HSPPPIPLLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 120

Query: 121 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 180
           PEF+EKMEANYHERAV++G LV+SACGFDSVPAELG +FNS+QWVG++APNRIEAYLSLE
Sbjct: 121 PEFMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQTAPNRIEAYLSLE 180

Query: 181 SKNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 240
           S  KIV NFGTFESAVLGVANAD+L++LRRSR R+ RP IPG PPPKGPT+EH KE  LW
Sbjct: 181 SSKKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPKIPGPPPPKGPTIEHKKEIGLW 240

Query: 241 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 300
           +VRLPSADSTVVRRTLS+L ENP GLPGVNE   EIEQR TFWSS+KPAHFGVKIG KSL
Sbjct: 241 SVRLPSADSTVVRRTLSTLVENPQGLPGVNESAYEIEQRKTFWSSVKPAHFGVKIGTKSL 300

Query: 301 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 360
           I IL++I VG+ IGLL ++S G WLLLTFPS+FSLGWFRK GPSE+EV+SASFKMWFVGH
Sbjct: 301 IGILRIIAVGMFIGLLGKTSLGRWLLLTFPSVFSLGWFRKKGPSEEEVNSASFKMWFVGH 360

Query: 361 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 420
           GF ++N   + E +M+IVTRVMGPE GY+ T IILVQCAL+VLS+RE LPKGGVLTPGIV
Sbjct: 361 GFRSSNNEANVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSRREALPKGGVLTPGIV 420

Query: 421 FGPTDLQKRLEENEICFDVISKHA 445
           FGPTDLQ+RL+EN I FDVISK+A
Sbjct: 421 FGPTDLQQRLQENGISFDVISKNA 442

BLAST of CmaCh11G011550 vs. NCBI nr
Match: gi|700204396|gb|KGN59529.1| (hypothetical protein Csa_3G824740 [Cucumis sativus])

HSP 1 Score: 723.8 bits (1867), Expect = 1.9e-205
Identity = 352/444 (79.28%), Postives = 395/444 (88.96%), Query Frame = 1

Query: 1   MEAPLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP 60
           ME   YD IIL A+GFTGKYVVR+AL+FLNPS  SPLKSFALAGRN TKL++TL+WAAHP
Sbjct: 34  MEGTPYDLIILGATGFTGKYVVREALRFLNPS--SPLKSFALAGRNLTKLTQTLQWAAHP 93

Query: 61  NSPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGE 120
           +SPP IPLL ADI+DPQSIHRLCTQTKLILNC GPFRRYGEPVVAACVETGCDYLDICGE
Sbjct: 94  HSPPPIPLLIADIADPQSIHRLCTQTKLILNCVGPFRRYGEPVVAACVETGCDYLDICGE 153

Query: 121 PEFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLE 180
           PEF+EKMEANYHERAV++G LV+SACGFDSVPAELG +FNS+QWVG++APNRIEAYLSLE
Sbjct: 154 PEFMEKMEANYHERAVQSGALVVSACGFDSVPAELGLMFNSRQWVGQTAPNRIEAYLSLE 213

Query: 181 SKNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLW 240
           S  KIV NFGTFESAVLGVANAD+L++LRRSR R+ RP IPG PPPKGPT+EH KE  LW
Sbjct: 214 SSKKIVGNFGTFESAVLGVANADQLLKLRRSRPRKPRPKIPGPPPPKGPTIEHKKEIGLW 273

Query: 241 AVRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSL 300
           +VRLPSADSTVVRRTLS+L ENP GLPGVNE   EIEQR TFWSS+KPAHFGVKIG KSL
Sbjct: 274 SVRLPSADSTVVRRTLSTLVENPQGLPGVNESAYEIEQRKTFWSSVKPAHFGVKIGTKSL 333

Query: 301 ICILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGH 360
           I IL++I VG+ IGLL ++S G WLLLTFPS+FSLGWFRK GPSE+EV+SASFKMWFVGH
Sbjct: 334 IGILRIIAVGMFIGLLGKTSLGRWLLLTFPSVFSLGWFRKKGPSEEEVNSASFKMWFVGH 393

Query: 361 GFSNNNGGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIV 420
           GF ++N   + E +M+IVTRVMGPE GY+ T IILVQCAL+VLS+RE LPKGGVLTPGIV
Sbjct: 394 GFRSSNNEANVEPEMEIVTRVMGPEIGYLTTPIILVQCALIVLSRREALPKGGVLTPGIV 453

Query: 421 FGPTDLQKRLEENEICFDVISKHA 445
           FGPTDLQ+RL+EN I FDVISK+A
Sbjct: 454 FGPTDLQQRLQENGISFDVISKNA 475

BLAST of CmaCh11G011550 vs. NCBI nr
Match: gi|1009125839|ref|XP_015879828.1| (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Ziziphus jujuba])

HSP 1 Score: 667.9 bits (1722), Expect = 1.2e-188
Identity = 324/446 (72.65%), Postives = 379/446 (84.98%), Query Frame = 1

Query: 3   APLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHP-N 62
           +P YD +I  ASGFTGKYVV++ALKFLN +PSSPLKS ALAGRNPTKL ETLKWAAHP N
Sbjct: 14  SPQYDVVIFGASGFTGKYVVQEALKFLN-TPSSPLKSLALAGRNPTKLRETLKWAAHPQN 73

Query: 63  SPPSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEP 122
            PP IP+LTAD +DPQS+ RLCTQTKLILNC GPFR YGEPVVAAC ETGCDYLDICGEP
Sbjct: 74  PPPHIPILTADTTDPQSLRRLCTQTKLILNCVGPFRLYGEPVVAACAETGCDYLDICGEP 133

Query: 123 EFIEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLES 182
           EF+E+MEA YHE+AVE G LV+SACGFDSVPAELGF+FNS+QWV  + PNR+EAYLSLES
Sbjct: 134 EFMERMEAKYHEKAVETGSLVVSACGFDSVPAELGFMFNSRQWVAPAVPNRVEAYLSLES 193

Query: 183 KNKIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWA 242
           + +IV N GT+ESAVLGVANAD+L++LRRSR RR+RP IPG PPPKGPT+EH K   L A
Sbjct: 194 EKRIVGNLGTYESAVLGVANADKLLELRRSRPRRARPVIPGPPPPKGPTIEHQKNIGLHA 253

Query: 243 VRLPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLI 302
           V+LPSADS VVRRTL++L ENP GLPG+NE  E+IE+R  FWS++KPAHFGVKIG KSL+
Sbjct: 254 VKLPSADSIVVRRTLATLTENPDGLPGLNESAEQIERRKAFWSTVKPAHFGVKIGTKSLL 313

Query: 303 CILQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHG 362
            I ++I VGI IG+L + +FG WLLL FPS FSLGWF+K GP+EDEV SASFKMWFVGHG
Sbjct: 314 GIYRIIAVGIFIGILGKVAFGRWLLLKFPSFFSLGWFKKKGPTEDEVRSASFKMWFVGHG 373

Query: 363 FSNNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGI 422
            S+ N    G+ + DM+IVTRVMGPE GY+ T IIL+QCAL+VL++R+ LPKGGV  PGI
Sbjct: 374 VSDINLISQGNSKPDMEIVTRVMGPEIGYLTTPIILLQCALIVLNQRDNLPKGGVYPPGI 433

Query: 423 VFGPTDLQKRLEENEICFDVISKHAL 446
           VFGPTDLQ+RL++N I FDVISK+A+
Sbjct: 434 VFGPTDLQERLQQNGISFDVISKNAI 458

BLAST of CmaCh11G011550 vs. NCBI nr
Match: gi|694379273|ref|XP_009365835.1| (PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 [Pyrus x bretschneideri])

HSP 1 Score: 661.8 bits (1706), Expect = 8.8e-187
Identity = 321/444 (72.30%), Postives = 375/444 (84.46%), Query Frame = 1

Query: 4   PLYDFIILAASGFTGKYVVRQALKFLNPSPSSPLKSFALAGRNPTKLSETLKWAAHPNSP 63
           P YD IIL ASGFTGKYVVR+ALKFLN +P+SPLKS ALAGRNP KL++TLKWAAHPNSP
Sbjct: 13  PSYDLIILGASGFTGKYVVREALKFLN-TPASPLKSLALAGRNPAKLTQTLKWAAHPNSP 72

Query: 64  PSIPLLTADISDPQSIHRLCTQTKLILNCAGPFRRYGEPVVAACVETGCDYLDICGEPEF 123
           PSIP++TAD +DP S+HRLCTQT LILNC GPFR YG+PVVAAC ETGCDYLDICGEPEF
Sbjct: 73  PSIPIITADTTDPPSLHRLCTQTTLILNCVGPFRLYGDPVVAACAETGCDYLDICGEPEF 132

Query: 124 IEKMEANYHERAVENGCLVISACGFDSVPAELGFIFNSKQWVGESAPNRIEAYLSLESKN 183
           +E+MEA YH +A E G LV+SACGFDSVPAE G +FNS+QWV  + PNR+EAY+SLES+ 
Sbjct: 133 MERMEAQYHGKAAETGSLVVSACGFDSVPAEFGLMFNSRQWVDPAVPNRVEAYVSLESEK 192

Query: 184 KIVANFGTFESAVLGVANADRLVQLRRSRSRRSRPTIPGCPPPKGPTVEHNKETSLWAVR 243
           +IV N GT+ESA LGVANAD+L +LRRSR RR+RP IPG PPPKGPT+E  K+  LWAV+
Sbjct: 193 RIVGNLGTYESAGLGVANADKLQELRRSRPRRARPAIPG-PPPKGPTIEQQKDIGLWAVK 252

Query: 244 LPSADSTVVRRTLSSLAENPHGLPGVNERVEEIEQRTTFWSSMKPAHFGVKIGHKSLICI 303
           LPSAD+ +VRRTL  L ENPHGLPG NE +E IE+R  FWS++KPAHFGVKIG KSL+ I
Sbjct: 253 LPSADAIIVRRTLGFLTENPHGLPGRNENLEHIEKREAFWSTVKPAHFGVKIGTKSLLGI 312

Query: 304 LQMIVVGIIIGLLSRSSFGTWLLLTFPSIFSLGWFRKNGPSEDEVDSASFKMWFVGHGFS 363
           L++++VGI IGLL R SFG WLLL FPS FS+GWFRK GPSEDEV SASFKMWFVG GFS
Sbjct: 313 LRIMMVGIFIGLLGRFSFGRWLLLKFPSFFSVGWFRKKGPSEDEVRSASFKMWFVGRGFS 372

Query: 364 NNN--GGGSRELDMKIVTRVMGPESGYVATSIILVQCALVVLSKREILPKGGVLTPGIVF 423
           +++    GSR+ +M+I+TRVMGPE GY+ T IIL+QCAL+VLS+R+ LPKGGVL  GIVF
Sbjct: 373 DSSLVSQGSRKPNMEIITRVMGPEIGYLTTPIILLQCALIVLSQRDNLPKGGVLPAGIVF 432

Query: 424 GPTDLQKRLEENEICFDVISKHAL 446
           GPT LQ+RL+EN I FDVISK AL
Sbjct: 433 GPTGLQERLQENGISFDVISKKAL 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SCPDL_ARATH5.9e-17566.29Probable mitochondrial saccharopine dehydrogenase-like oxidoreductase At5g39410 ... [more]
SCPDL_MOUSE2.8e-6834.62Saccharopine dehydrogenase-like oxidoreductase OS=Mus musculus GN=Sccpdh PE=1 SV... [more]
SCPDL_RAT2.4e-6734.16Saccharopine dehydrogenase-like oxidoreductase OS=Rattus norvegicus GN=Sccpdh PE... [more]
SCPDL_HUMAN3.1e-6734.54Saccharopine dehydrogenase-like oxidoreductase OS=Homo sapiens GN=SCCPDH PE=1 SV... [more]
SCPDL_BOVIN3.4e-6634.31Saccharopine dehydrogenase-like oxidoreductase OS=Bos taurus GN=SCCPDH PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LF70_CUCSA1.3e-20579.28Uncharacterized protein OS=Cucumis sativus GN=Csa_3G824740 PE=4 SV=1[more]
M5X4V6_PRUPE2.3e-18671.91Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005544mg PE=4 SV=1[more]
A0A067JTH7_JATCU2.0e-18571.30Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19896 PE=4 SV=1[more]
W9QQU6_9ROSA2.4e-18369.73Putative mitochondrial saccharopine dehydrogenase-like oxidoreductase OS=Morus n... [more]
B9H0F4_POPTR4.1e-18369.28Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s12470g PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT5G39410.13.3e-17666.29 Saccharopine dehydrogenase [more]
Match NameE-valueIdentityDescription
gi|659085331|ref|XP_008443363.1|2.8e-20980.41PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
gi|449437814|ref|XP_004136685.1|1.9e-20579.28PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
gi|700204396|gb|KGN59529.1|1.9e-20579.28hypothetical protein Csa_3G824740 [Cucumis sativus][more]
gi|1009125839|ref|XP_015879828.1|1.2e-18872.65PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
gi|694379273|ref|XP_009365835.1|8.8e-18772.30PREDICTED: probable mitochondrial saccharopine dehydrogenase-like oxidoreductase... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005097Sacchrp_dh_NADP
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006333 chromatin assembly or disassembly
biological_process GO:0007623 circadian rhythm
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005774 vacuolar membrane
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G011550.1CmaCh11G011550.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005097Saccharopine dehydrogenase, NADP binding domainPFAMPF03435Sacchrp_dh_NADPcoord: 13..143
score: 2.3
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 10..153
score: 7.2
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 9..159
score: 1.6
NoneNo IPR availablePANTHERPTHR12286UNCHARACTERIZEDcoord: 1..255
score: 7.6E-177coord: 277..448
score: 7.6E
NoneNo IPR availablePANTHERPTHR12286:SF5SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASEcoord: 277..448
score: 7.6E-177coord: 1..255
score: 7.6E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None