CmaCh10G000890 (gene) Cucurbita maxima (Rimu)

NameCmaCh10G000890
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCytochrome P450 family 71 protein
LocationCma_Chr10 : 365303 .. 367138 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTCATGCAAACGTCCATGTAGAGTGTGAAGCCGAGCTATCTTGTTTTCATTTATGGTTGTAGAGAAGTTTGAGTCTCACAGATATTATTTCCTTCTAAGAACACCAAAATCTTCAATTTCTGAAATTTCTGGATTCAAATGGAAGGTGATCTCCTTGTTGCGTTGATTGGTCACCCAGTTTCCGTCGCTGGTTTCATTGTCCTCTTCATCTGGCTTTTGTTTCGTCAAAAATCTTCATCACAAGAAGAACAATCCTCGCCCCCATCTCCACCAACAAAGCTTCCAATTTTCGGACATCTTTTGAAGCTTGGCTCCCATCCTCATATCACTCTCCAGAGCTACGCCCGCCGCCATGGCCCTCTGTTTCTCCTCCACCTCGGCTCCAAGCCCACCATCGTGGTCTCTTCATCCGAAATAGCTCGTGATGTCATGAAAACCCACGATCTTGTCTTTGCCAACCGACCCAAATCGAGTATTTCCGATAAGCTTCTTTACAACGCCAGCGACATATCAGCTGCTCCTTATGGCGAGTATTGGAGGCAGATGAGAAGCGTTGGCGTACTTCACCTTTTAAGTAACAAAAGGGTTCAATCCTTCCGCTCTGTGAGAGAAGAAGAGGTCAAATTAATGATCGAGAAGATCCAACGGAACCCAATATCGGTGAATTTGACTGAAACCTTCTCTGGACTGACCAACAACGTGGTTTGCAGAGTGGCTTTAGGGGCAAAATATGGAGTGGGAGAAGATTGGATTAAGTTAAGGTCTCTTCTATTGGAATTTCTGGAGTTGTTAGGGAGCTTTGGCGTCAGAGACTTCATTCCTTGGCTGGGTTGGATCGATGGTATCAGTGGGCTGGACGCCAAAGCAAAGCGGGTAGCCAAAGAGCTGGATGTTTTCCTAGATAGAGTGATTCAAGATCATGTCGATTCGGAAAACAGAGACGAGAATTCTGATAAGCACATGGACCTGGTCGATGTTCTGCTTTGGATACAGAGAAACAACTCCATTGGTTTCCCCCTTGAGATGGATAGCATAAAAGCTTTAATCTTGGTAAGCCAATCTTGCATTCATTTTTCACCCATCAACTTTGGTTGTGGTTTGGTTTTGATTGTGGTTTCGATTACAGGACATGTTTTCAGCTGGCACAGACACAACTTCCACAGTATTGGAGTGGGCAATGTCAGAATTGTTCAAACACCCAGAAGCCATGAAGAAATTGAAGAGTGAGATCAGAGAAATATCAGGCGATCAGTACGTAAACGAAGATGATCTAGACAAAATGCATTACTTGAAGGCAGTAATGAAGGAAACGTTGCGTCTACACCCACCAATCCCACTTCTTGTCCCGAGAGAATCGACCCAATGCATAAAGCTAAGGGGCTACGATATTAAACCCAAGACCCGAGTGATGATCAATGCATGGGCTATTGGGAGAGACCCAGAAGTGTGGGAAGAAGCAGAGGAGTTTCGGCCAGAAAGGTTTATGAACAGTTCGATAGATTTCAAGGGTCAGGATTTTGAGTTAATCCCATTTGGCGCAGGGAGGAGAGGATGTCCTGGAGTTGAGTTTGCAACGGTGATAAATGAGATAGGTTTAGCAAATCTGGTGCATAAATTTGAATGGATGTTGCCAAGTGGTGAGGATTTAGACATGACGGGGGCGTTTGGGTTAACCATTCATAGGAAACATCCTCTTGTAGCCACGGCAACTCCTTGTTAGAGTGTTGGAGGCTTTATTGCATGTGTTTTTGTTGCCTTTTATCTTTATTGTATCCATTATGATTATGTAGGCAGCCTATTAATGAGAACTGGACTCAAGAATATAGAACAGAGC

mRNA sequence

GCTTCATGCAAACGTCCATGTAGAGTGTGAAGCCGAGCTATCTTGTTTTCATTTATGGTTGTAGAGAAGTTTGAGTCTCACAGATATTATTTCCTTCTAAGAACACCAAAATCTTCAATTTCTGAAATTTCTGGATTCAAATGGAAGGTGATCTCCTTGTTGCGTTGATTGGTCACCCAGTTTCCGTCGCTGGTTTCATTGTCCTCTTCATCTGGCTTTTGTTTCGTCAAAAATCTTCATCACAAGAAGAACAATCCTCGCCCCCATCTCCACCAACAAAGCTTCCAATTTTCGGACATCTTTTGAAGCTTGGCTCCCATCCTCATATCACTCTCCAGAGCTACGCCCGCCGCCATGGCCCTCTGTTTCTCCTCCACCTCGGCTCCAAGCCCACCATCGTGGTCTCTTCATCCGAAATAGCTCGTGATGTCATGAAAACCCACGATCTTGTCTTTGCCAACCGACCCAAATCGAGTATTTCCGATAAGCTTCTTTACAACGCCAGCGACATATCAGCTGCTCCTTATGGCGAGTATTGGAGGCAGATGAGAAGCGTTGGCGTACTTCACCTTTTAAGTAACAAAAGGGTTCAATCCTTCCGCTCTGTGAGAGAAGAAGAGGTCAAATTAATGATCGAGAAGATCCAACGGAACCCAATATCGGTGAATTTGACTGAAACCTTCTCTGGACTGACCAACAACGTGGTTTGCAGAGTGGCTTTAGGGGCAAAATATGGAGTGGGAGAAGATTGGATTAAGTTAAGGTCTCTTCTATTGGAATTTCTGGAGTTGTTAGGGAGCTTTGGCGTCAGAGACTTCATTCCTTGGCTGGGTTGGATCGATGGTATCAGTGGGCTGGACGCCAAAGCAAAGCGGGTAGCCAAAGAGCTGGATGTTTTCCTAGATAGAGTGATTCAAGATCATGTCGATTCGGAAAACAGAGACGAGAATTCTGATAAGCACATGGACCTGGTCGATGTTCTGCTTTGGATACAGAGAAACAACTCCATTGGTTTCCCCCTTGAGATGGATAGCATAAAAGCTTTAATCTTGGACATGTTTTCAGCTGGCACAGACACAACTTCCACAGTATTGGAGTGGGCAATGTCAGAATTGTTCAAACACCCAGAAGCCATGAAGAAATTGAAGAGTGAGATCAGAGAAATATCAGGCGATCAGTACGTAAACGAAGATGATCTAGACAAAATGCATTACTTGAAGGCAGTAATGAAGGAAACGTTGCGTCTACACCCACCAATCCCACTTCTTGTCCCGAGAGAATCGACCCAATGCATAAAGCTAAGGGGCTACGATATTAAACCCAAGACCCGAGTGATGATCAATGCATGGGCTATTGGGAGAGACCCAGAAGTGTGGGAAGAAGCAGAGGAGTTTCGGCCAGAAAGGTTTATGAACAGTTCGATAGATTTCAAGGGTCAGGATTTTGAGTTAATCCCATTTGGCGCAGGGAGGAGAGGATGTCCTGGAGTTGAGTTTGCAACGGTGATAAATGAGATAGGTTTAGCAAATCTGGTGCATAAATTTGAATGGATGTTGCCAAGTGGTGAGGATTTAGACATGACGGGGGCGTTTGGGTTAACCATTCATAGGAAACATCCTCTTGTAGCCACGGCAACTCCTTGTTAGAGTGTTGGAGGCTTTATTGCATGTGTTTTTGTTGCCTTTTATCTTTATTGTATCCATTATGATTATGTAGGCAGCCTATTAATGAGAACTGGACTCAAGAATATAGAACAGAGC

Coding sequence (CDS)

ATGGAAGGTGATCTCCTTGTTGCGTTGATTGGTCACCCAGTTTCCGTCGCTGGTTTCATTGTCCTCTTCATCTGGCTTTTGTTTCGTCAAAAATCTTCATCACAAGAAGAACAATCCTCGCCCCCATCTCCACCAACAAAGCTTCCAATTTTCGGACATCTTTTGAAGCTTGGCTCCCATCCTCATATCACTCTCCAGAGCTACGCCCGCCGCCATGGCCCTCTGTTTCTCCTCCACCTCGGCTCCAAGCCCACCATCGTGGTCTCTTCATCCGAAATAGCTCGTGATGTCATGAAAACCCACGATCTTGTCTTTGCCAACCGACCCAAATCGAGTATTTCCGATAAGCTTCTTTACAACGCCAGCGACATATCAGCTGCTCCTTATGGCGAGTATTGGAGGCAGATGAGAAGCGTTGGCGTACTTCACCTTTTAAGTAACAAAAGGGTTCAATCCTTCCGCTCTGTGAGAGAAGAAGAGGTCAAATTAATGATCGAGAAGATCCAACGGAACCCAATATCGGTGAATTTGACTGAAACCTTCTCTGGACTGACCAACAACGTGGTTTGCAGAGTGGCTTTAGGGGCAAAATATGGAGTGGGAGAAGATTGGATTAAGTTAAGGTCTCTTCTATTGGAATTTCTGGAGTTGTTAGGGAGCTTTGGCGTCAGAGACTTCATTCCTTGGCTGGGTTGGATCGATGGTATCAGTGGGCTGGACGCCAAAGCAAAGCGGGTAGCCAAAGAGCTGGATGTTTTCCTAGATAGAGTGATTCAAGATCATGTCGATTCGGAAAACAGAGACGAGAATTCTGATAAGCACATGGACCTGGTCGATGTTCTGCTTTGGATACAGAGAAACAACTCCATTGGTTTCCCCCTTGAGATGGATAGCATAAAAGCTTTAATCTTGGACATGTTTTCAGCTGGCACAGACACAACTTCCACAGTATTGGAGTGGGCAATGTCAGAATTGTTCAAACACCCAGAAGCCATGAAGAAATTGAAGAGTGAGATCAGAGAAATATCAGGCGATCAGTACGTAAACGAAGATGATCTAGACAAAATGCATTACTTGAAGGCAGTAATGAAGGAAACGTTGCGTCTACACCCACCAATCCCACTTCTTGTCCCGAGAGAATCGACCCAATGCATAAAGCTAAGGGGCTACGATATTAAACCCAAGACCCGAGTGATGATCAATGCATGGGCTATTGGGAGAGACCCAGAAGTGTGGGAAGAAGCAGAGGAGTTTCGGCCAGAAAGGTTTATGAACAGTTCGATAGATTTCAAGGGTCAGGATTTTGAGTTAATCCCATTTGGCGCAGGGAGGAGAGGATGTCCTGGAGTTGAGTTTGCAACGGTGATAAATGAGATAGGTTTAGCAAATCTGGTGCATAAATTTGAATGGATGTTGCCAAGTGGTGAGGATTTAGACATGACGGGGGCGTTTGGGTTAACCATTCATAGGAAACATCCTCTTGTAGCCACGGCAACTCCTTGTTAG

Protein sequence

MEGDLLVALIGHPVSVAGFIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTETFSGLTNNVVCRVALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTGAFGLTIHRKHPLVATATPC
BLAST of CmaCh10G000890 vs. Swiss-Prot
Match: C71AP_ARATH (Cytochrome P450 71A25 OS=Arabidopsis thaliana GN=CYP71A25 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.6e-144
Identity = 253/486 (52.06%), Postives = 353/486 (72.63%), Query Frame = 1

Query: 21  VLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLHL 80
           ++F+ +LF +K  S ++  +PPSPP  LP+ G+L +LG H H +L   +RR+GPL LLHL
Sbjct: 11  IIFMTILFLKKQLSGKKGKTPPSPPG-LPLIGNLHQLGRHTHRSLCDLSRRYGPLMLLHL 70

Query: 81  GSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSVG 140
           G  P ++VSS+++A++++KTHD  FANRP+S +S KLLYN  D+++APYGEYWRQM+SV 
Sbjct: 71  GRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQMKSVC 130

Query: 141 VLHLLSNKRVQSFRSVREEEVKLMIEKIQR-NPISVNLTETFSGLTNNVVCRVALGAKYG 200
           V+HLLSNK V+SFR VREEE+ LM+ KI++ + +  N+++    LTN+V+CRVALG KYG
Sbjct: 131 VIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICRVALGRKYG 190

Query: 201 VGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQ 260
              D+ KL   L    ELLG+F +  F+PWL W+D I G DA+  ++ K+LD F ++V+Q
Sbjct: 191 GETDFKKLTDRL---SELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQ 250

Query: 261 DHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLE 320
           DH D + RD       DL+D LL ++R  S GF +E  SIKA+ LD+F  G+DT+ T+LE
Sbjct: 251 DHEDGDRRDGT-----DLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLE 310

Query: 321 WAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLVP 380
           WAM+EL +HP+++ +L+ E+R I  G   V+EDD+  M YLKAV+KE LRLHPP P++ P
Sbjct: 311 WAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPMMAP 370

Query: 381 RESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFEL 440
            EST+ +KLR Y I   T+VM+NAWAIGR+   W  +AEEF+PER +++S+DF+GQ+FEL
Sbjct: 371 HESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLDTSVDFRGQNFEL 430

Query: 441 IPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLP--SGED-LDMTGAFGLTIHRKHPL 500
           +PFGAGRR CP V FA V+NE+ LANLVH F+W LP  S ED  D+  + G ++HR+ PL
Sbjct: 431 LPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTDVAESSGFSVHREFPL 487

BLAST of CmaCh10G000890 vs. Swiss-Prot
Match: C71AO_ARATH (Cytochrome P450 71A24 OS=Arabidopsis thaliana GN=CYP71A24 PE=2 SV=3)

HSP 1 Score: 512.3 bits (1318), Expect = 6.0e-144
Identity = 256/485 (52.78%), Postives = 340/485 (70.10%), Query Frame = 1

Query: 21  VLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLHL 80
           ++ I +LF +K S  ++ ++PPSPP +LP+  +L +LG HPH +L S + R+GPL LLH 
Sbjct: 14  IILITILFFKKQSRGKKSNAPPSPP-RLPLIRNLHQLGRHPHRSLCSLSHRYGPLMLLHF 73

Query: 81  GSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSVG 140
           GS P +VVSS++ A+DV+KTHD VFA+RP+S I DK+ YN  D++ APYGEYWRQM+SV 
Sbjct: 74  GSVPVLVVSSADAAKDVLKTHDRVFASRPRSKIFDKIFYNGRDVALAPYGEYWRQMKSVC 133

Query: 141 VLHLLSNKRVQSFRSVREEEVKLMIEKIQ-RNPISVNLTETFSGLTNNVVCRVALGAKYG 200
           VLHL SNK V+SFR VR+EE+ LMIEKI+  + + +NL+E    LTNNV+CRVALG KYG
Sbjct: 134 VLHLFSNKMVRSFRDVRQEEISLMIEKIRISSSLRINLSEILVNLTNNVICRVALGRKYG 193

Query: 201 VGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQ 260
              D+   + L+     LLG F V  ++ WL WID I GLD +  +++ +LD FL+RV+Q
Sbjct: 194 GKTDF---KDLMKRLTRLLGEFSVGSYVSWLAWIDWIRGLDGQLIKISNDLDEFLERVVQ 253

Query: 261 DHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLE 320
           DHVD +          D VD LL I+R  S+GF ++  SIKA+ILD+F    DTT T+LE
Sbjct: 254 DHVDGDGHKN------DFVDFLLTIEREKSVGFEIDRLSIKAIILDVFVGDMDTTYTLLE 313

Query: 321 WAMSELFKHPEAMKKLKSEIREISGDQY-VNEDDLDKMHYLKAVMKETLRLHPPIPLLVP 380
           WAM+EL  H E + +L+ E+R +  D+  V+EDDL  M YLKAV+KETLRLHPP+PL+VP
Sbjct: 314 WAMTELLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLHPPLPLMVP 373

Query: 381 RESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFEL 440
            EST  +KLR Y I   T VMINAWAIGR+   W  +AEEFRPER +NS +D++GQD EL
Sbjct: 374 HESTHDVKLRDYHIPAGTHVMINAWAIGREAATWGPDAEEFRPERHLNSYVDYRGQDTEL 433

Query: 441 IPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGE---DLDMTGAFGLTIHRKHPL 500
           +PFGAGRR CP + FA V++E+ LANLVH+F+W LP        D+  + G+ +HR  PL
Sbjct: 434 VPFGAGRRICPAISFAVVLDEVVLANLVHQFDWTLPEESTEYQTDVAESTGMAVHRMFPL 488

BLAST of CmaCh10G000890 vs. Swiss-Prot
Match: C71A4_SOLME (Cytochrome P450 71A4 OS=Solanum melongena GN=CYP71A4 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.0e-143
Identity = 260/492 (52.85%), Postives = 345/492 (70.12%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSS--PPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFL 79
           ++LFI+LL     ++ + Q+   PPSP  KLPI G+L +LGSHPH +L+  ++++GP+ L
Sbjct: 14  LLLFIFLLIHHCFTTSKTQNMFLPPSP-RKLPIIGNLHQLGSHPHRSLRKLSQKYGPVML 73

Query: 80  LHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMR 139
           LHLGSKP IV SS + ARD++KTHD V+A RPK SI+D LLY + D+  +P+GEYW Q+R
Sbjct: 74  LHLGSKPVIVASSVDAARDILKTHDHVWATRPKYSIADSLLYGSKDVGFSPFGEYWWQVR 133

Query: 140 SVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQR--NPISVNLTETFSGLTNNVVCRVALG 199
           S+ VLHLLSNKRVQS+R VREEE   MIEKI++  +   +NL E    LTNN+  RVALG
Sbjct: 134 SIVVLHLLSNKRVQSYRDVREEETANMIEKIRQGCDASVINLGEHLCFLTNNITSRVALG 193

Query: 200 AKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLD 259
             Y   E  I  + +L +FL+LL +F V D+IPWL W++ I+GLD K +++AK+LD FLD
Sbjct: 194 RTYDERESGIDAKDILEQFLQLLDTFNVGDYIPWLKWVNKITGLDTKVEKIAKKLDTFLD 253

Query: 260 RVIQDHVDSENRDEN--SDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDT 319
            VI++H+    ++E   +D+  D VDVLL IQ      FPL+ DS+KA++LD F+AGTDT
Sbjct: 254 SVIEEHIIRNKKEEYAITDEAKDFVDVLLEIQNGKETDFPLQRDSLKAILLDAFAAGTDT 313

Query: 320 TSTVLEWAMSELFKHPEAMKKLKSEIREIS-GDQYVNEDDLDKMHYLKAVMKETLRLHPP 379
             T L+W M+++ + P AMK L++E+R ++ G   + EDDL  M YL+AV+KE+LRLHPP
Sbjct: 314 IYTNLDWTMADVLRQPRAMKTLQNEVRGLAQGKSEITEDDLKNMQYLRAVIKESLRLHPP 373

Query: 380 IPLLVPRESTQCIKLRG-YDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSIDFK 439
             LLVPRES + +KL G Y I  +T+ +IN WAIGRDP  WE  EEF PERF+N+ ID K
Sbjct: 374 NSLLVPRESMEDVKLLGYYHIPARTQALINVWAIGRDPLSWENPEEFCPERFLNNDIDMK 433

Query: 440 GQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG---EDLDMTGAFGLTI 499
           G  FEL+PFG+GRRGCPG  FA  + E+ LA LVHKF + LP G   EDLDMT   G+  
Sbjct: 434 GLKFELLPFGSGRRGCPGSSFAIAVIELALARLVHKFNFALPKGTKPEDLDMTECTGIAT 493

Query: 500 HRKHPLVATATP 501
            RK PL   ATP
Sbjct: 494 RRKSPLPVVATP 504

BLAST of CmaCh10G000890 vs. Swiss-Prot
Match: C71AQ_ARATH (Cytochrome P450 71A26 OS=Arabidopsis thaliana GN=CYP71A26 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 251/486 (51.65%), Postives = 355/486 (73.05%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           I+  + ++  +K    +++++ PSPP  LP+ G+L +LG HPH +L S + R+GPL LLH
Sbjct: 11  IIFVVTIIIFRKQKRGKKRNTLPSPPG-LPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLH 70

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
            G  P +VVSS+E+ARDV+KTHD VFA+RP+S I +KLLY+  D+++APYGEYWRQM+SV
Sbjct: 71  FGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSV 130

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRN-PISVNLTETFSGLTNNVVCRVALGAKY 199
            VLHL SNK V+SFR VREEE+ LM+EKI+++  + VNL++    LTN+V+C+VALG KY
Sbjct: 131 CVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLTNDVICKVALGRKY 190

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
           G   D+   + L+    +LLG+F V  ++PWL WID I GLD + ++ A ++D F +RV+
Sbjct: 191 GGETDF---KELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVV 250

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDHVD  NRD       D VDVLL IQR+ ++GF +   SIKA+++++F  GTDT+ST++
Sbjct: 251 QDHVDG-NRDMT-----DFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTLM 310

Query: 320 EWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           EWAM+EL +HP+ +K+L+ E+R I  D+  V+E+++  M YLKAV+KE LRLHPP+PL+V
Sbjct: 311 EWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLHPPLPLMV 370

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P ESTQ ++L  + I   T+V+INAWAIGR+   W  + EEFRPER ++SS+D++GQ FE
Sbjct: 371 PHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDSSVDYRGQAFE 430

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWML--PSGED-LDMTGAFGLTIHRKHP 499
           LIPFG+GRR CP + FA V+NE+ LANLVH+F+W L   S ED  ++  + G+ IHR  P
Sbjct: 431 LIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQTEVAESTGIAIHRMFP 486

BLAST of CmaCh10G000890 vs. Swiss-Prot
Match: C71AM_ARATH (Cytochrome P450 71A22 OS=Arabidopsis thaliana GN=CYP71A22 PE=2 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 8.1e-141
Identity = 249/483 (51.55%), Postives = 344/483 (71.22%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           +++FI +LF  K    ++ ++P SPP +LP+ G+L +LG HPH +L S + R+GPL LL 
Sbjct: 12  LIIFITILFFIKQKKGKKSNTPASPP-RLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLR 71

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
            G  P +VVSS+++ARD++KT+D VFA+RP+S I +K+ Y A D++ APYGEYWRQM+SV
Sbjct: 72  FGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSV 131

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNP-ISVNLTETFSGLTNNVVCRVALGAKY 199
            VLHLL+NK V+SFR+VR+EE+ LM+EKIQ++  + VNL+E    LTN+V+ RVALG KY
Sbjct: 132 CVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQVNLSELLGSLTNDVISRVALGRKY 191

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
               D+   + L+    +LLG F V  ++PWL WID ISGLD + K+   +LD FL++V+
Sbjct: 192 SDETDF---KELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVV 251

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDH D + +        D VDVLL IQR  S+GF ++  SIKA+ILD+   GTDT+  ++
Sbjct: 252 QDHEDGDAQ------RTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYALM 311

Query: 320 EWAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           EWAM+EL   PE + +L+ E+R I  G+  V+EDD+  M+YLKAV+KET+RLHPP+PL+V
Sbjct: 312 EWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLHPPLPLMV 371

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P ESTQ ++L  Y I   T+VMINAWAIGR+   W  +AE+FRPER +NSS+DF+G +FE
Sbjct: 372 PHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLNSSVDFRGHNFE 431

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTG---AFGLTIHRKHP 497
           LIPFGAGRR CP + FA ++ E+ LANLVH+++W LP     D T    + G+ IHR  P
Sbjct: 432 LIPFGAGRRICPAISFAVILIEVTLANLVHRYDWRLPEEYIEDQTNVAESTGMVIHRLFP 484

BLAST of CmaCh10G000890 vs. TrEMBL
Match: A0A061FZH7_THECC (Cytochrome P450, family 71, subfamily A, polypeptide 25, putative OS=Theobroma cacao GN=TCM_014500 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 8.9e-171
Identity = 307/511 (60.08%), Postives = 382/511 (74.76%), Query Frame = 1

Query: 7   VALIGHPVSVAGFIVLFIWLLFR--QKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHIT 66
           +  + +P   A   + F+ LL      S+S  + + PPSP +K PIFG+L  +G  PH +
Sbjct: 13  IFFLSYPWLFAILAIFFVVLLKHIFNPSTSNAKLALPPSP-SKFPIFGNLHNIGLQPHRS 72

Query: 67  LQSYARRHGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDI 126
           L++ A+R GPL LLHLGS P +VVSSS+ AR++MKTHDL FANRPKSS S KLLY+  D+
Sbjct: 73  LRALAQRFGPLMLLHLGSVPVLVVSSSDAAREIMKTHDLTFANRPKSSSSQKLLYDYKDV 132

Query: 127 SAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPIS---VNLTETF 186
           S+APYGEYWRQM+S  VLH LSNKRVQSFR VREEE  L+I+KI+ +  S   VNLTE F
Sbjct: 133 SSAPYGEYWRQMKSACVLHFLSNKRVQSFRVVREEETALIIDKIKESSSSALPVNLTEIF 192

Query: 187 SGLTNNVVCRVALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDA 246
           + LTNNV+CR ALG KY  GE   K + LL EF+ELLG F V D+IPWL W+  I+GL+A
Sbjct: 193 AMLTNNVICRAALGRKYSEGEGGKKFKKLLCEFVELLGGFNVGDYIPWLAWVSRINGLEA 252

Query: 247 KAKRVAKELDVFLDRVIQDHVDSENRDENS--------DKHMDLVDVLLWIQRNNSIGFP 306
           K ++VAKE D FL+ V+ +H+D   R  N         ++    VDVLL IQR N++GFP
Sbjct: 253 KMEKVAKEFDAFLEGVVDEHMDRYKRGHNDHDDTRLQHEEQKSFVDVLLEIQRENTVGFP 312

Query: 307 LEMDSIKALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDD 366
           LE  S+KALILDMF+AGTDTT TVLEWAM+EL +HPE MK+L+ E+R++ GD+ YV+EDD
Sbjct: 313 LERVSVKALILDMFAAGTDTTYTVLEWAMTELLRHPEIMKELQKEVRQVCGDETYVSEDD 372

Query: 367 LDKMHYLKAVMKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW 426
           LDKMHYLKAV+KE+LRLHPPIPLLVPR ST+ +K++GYDI   T+V+INAWAIGRDP  W
Sbjct: 373 LDKMHYLKAVIKESLRLHPPIPLLVPRISTEDVKIKGYDIARGTQVIINAWAIGRDPLSW 432

Query: 427 EEAEEFRPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWML 486
            + EEF PER +N+SIDFKG DFELIPFG+GRR CPG+ FA  INE+ LANLVHKF+W L
Sbjct: 433 AKPEEFLPERILNNSIDFKGHDFELIPFGSGRRICPGILFAMKINELFLANLVHKFDWSL 492

Query: 487 PSG---EDLDMTGAFGLTIHRKHPLVATATP 501
           P G   EDLDM  +FGLT HR++PL+A A P
Sbjct: 493 PGGAKEEDLDMGESFGLTTHRRYPLIAIANP 522

BLAST of CmaCh10G000890 vs. TrEMBL
Match: A5B9L0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035274 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 3.4e-162
Identity = 289/491 (58.86%), Postives = 370/491 (75.36%), Query Frame = 1

Query: 19  FIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLL 78
           FIVL +  LF    ++ +  + PPSPP +LP+ G++ +LG +P+ +L   AR +GPL LL
Sbjct: 17  FIVLLVRWLFSTPPTTHK--TLPPSPP-RLPVLGNMHQLGIYPYRSLLCLARCYGPLMLL 76

Query: 79  HLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRS 138
            LG   T+VVSS + A+++MKTHDL+FANRPK S+  +LLY+  D+S APYGEYWRQMRS
Sbjct: 77  QLGRVRTLVVSSPDAAQEIMKTHDLIFANRPKMSLGKRLLYDYKDVSVAPYGEYWRQMRS 136

Query: 139 VGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNP---ISVNLTETFSGLTNNVVCRVALG 198
           + VLHLLSNKRVQSF +VR EE+ L+I+KI+       S++L+  F  LTN+V+CRVA G
Sbjct: 137 ICVLHLLSNKRVQSFNTVRREEISLLIQKIEEFSSLSTSMDLSGMFMRLTNDVICRVAFG 196

Query: 199 AKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLD 258
            KY   E   K R LL EF+ELLG F V D+IPWL W++ ++G  AK +RVAKE D FLD
Sbjct: 197 RKYSGDERGKKFRRLLGEFVELLGGFNVGDYIPWLAWVEYVNGWXAKVERVAKEFDEFLD 256

Query: 259 RVIQDHVDSENRD-ENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTT 318
            V+++H+D         D   D VDVLL IQR+ ++GF ++ DSIKALILD+F+ GTDTT
Sbjct: 257 GVVEEHLDGGTGSIAKGDNEKDFVDVLLEIQRDGTLGFSMDRDSIKALILDIFAGGTDTT 316

Query: 319 STVLEWAMSELFKHPEAMKKLKSEIREIS-GDQYVNEDDLDKMHYLKAVMKETLRLHPPI 378
            TVLEWAM+EL +HP+AMK+L++E+R I+ G +++ EDDL+KMHYLKAV+KETLRLHPPI
Sbjct: 317 YTVLEWAMTELLRHPKAMKELQNEVRGITRGKEHITEDDLEKMHYLKAVIKETLRLHPPI 376

Query: 379 PLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSIDFKGQ 438
           PLLVPRES+Q + + GY I   T V+INAWA+GRDP  W+E EEFRPERF+N++IDFKG 
Sbjct: 377 PLLVPRESSQDVNIMGYHIPAGTMVIINAWAMGRDPMSWDEPEEFRPERFLNTNIDFKGH 436

Query: 439 DFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG---EDLDMTGAFGLTIHR 498
           DFELIPFGAGRRGCPG+ FA   NE+ LANLV+KF+W LP G   EDLDMT   GLTIHR
Sbjct: 437 DFELIPFGAGRRGCPGISFAMATNELVLANLVNKFDWALPDGARAEDLDMTECTGLTIHR 496

Query: 499 KHPLVATATPC 502
           K PL+A +TPC
Sbjct: 497 KFPLLAVSTPC 504

BLAST of CmaCh10G000890 vs. TrEMBL
Match: A0A059CXR3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B00204 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 2.2e-161
Identity = 285/490 (58.16%), Postives = 377/490 (76.94%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           ++LF WLL    S++Q+    PPSPP +LPI G+L ++GS PH +L + +R+HGPL LL 
Sbjct: 23  LLLFKWLL--SPSTTQKHHHPPPSPP-RLPILGNLHQIGSRPHSSLANLSRKHGPLMLLR 82

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
           LG  P +VVSS++ AR++MKTHDL+FANRPKS I +KL+Y+  D+S APYGEYWRQM+SV
Sbjct: 83  LGRVPVLVVSSADAAREIMKTHDLIFANRPKSKIFEKLVYDYKDVSMAPYGEYWRQMKSV 142

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQ-----RNPISVNLTETFSGLTNNVVCRVAL 199
            VL LLSNKRVQSFR VREEEV  +I  I+      +P +VNL E F+ LTN+VVCRVAL
Sbjct: 143 FVLQLLSNKRVQSFRLVREEEVANLIADIEGLLSSASPTTVNLGERFAALTNDVVCRVAL 202

Query: 200 GAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFL 259
           G KYG G+   + +++L+EF+ELLG+    D+IPWL W+D +SGLD +  +VAKELD FL
Sbjct: 203 GRKYG-GK---RFKAILMEFVELLGALSFGDYIPWLAWVDRVSGLDERLDKVAKELDGFL 262

Query: 260 DRVIQDHVDSENRDENS----DKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAG 319
           D V+++HV+     E      +   D VD+LL IQ+++S+GF ++  +IKALILD F+AG
Sbjct: 263 DEVVEEHVEKRMNCEGGVDVQEGKEDFVDILLSIQKDSSVGFSIDKITIKALILDAFAAG 322

Query: 320 TDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQY-VNEDDLDKMHYLKAVMKETLRL 379
           TDTT T LEWAM+EL +HP AM++ ++E+R I+G +  + E+DLD+M YLKAV+KETLR 
Sbjct: 323 TDTTYTALEWAMTELLRHPNAMRQAQAEVRRIAGHKSSITEEDLDQMCYLKAVIKETLRS 382

Query: 380 HPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSID 439
           HPPIPLL+PRESTQ  K+ GYD+  +TRV++NAWAIGRDP +W+  +EFRPERF+NSSI+
Sbjct: 383 HPPIPLLIPRESTQDTKVTGYDVAARTRVIVNAWAIGRDPSLWDSPDEFRPERFLNSSIN 442

Query: 440 FKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG-EDLDMTGAFGLTI 499
           F+G DFELIPFGAGRRGCPG+ FA V++E+ LANL+HKF+W LP G   LDMT + GLTI
Sbjct: 443 FQGHDFELIPFGAGRRGCPGIAFAIVVDELVLANLLHKFDWRLPDGVGQLDMTESTGLTI 502

BLAST of CmaCh10G000890 vs. TrEMBL
Match: A0A059CZ64_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B00199 PE=3 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 2.2e-161
Identity = 285/490 (58.16%), Postives = 377/490 (76.94%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           ++LF WLL    S++Q+    PPSPP +LPI G+L ++GS PH +L + +R+HGPL LL 
Sbjct: 23  LLLFKWLL--SPSTTQKHHHPPPSPP-RLPILGNLHQIGSRPHSSLANLSRKHGPLMLLR 82

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
           LG  P +VVSS++ AR++MKTHDL+FANRPKS I +KL+Y+  D+S APYGEYWRQM+SV
Sbjct: 83  LGRVPVLVVSSADAAREIMKTHDLIFANRPKSKIFEKLVYDYKDLSMAPYGEYWRQMKSV 142

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQ-----RNPISVNLTETFSGLTNNVVCRVAL 199
            VL LLSNKRVQSFR VREEEV  +I  I+      +P +VNL E F+ LTN+VVCRVAL
Sbjct: 143 FVLQLLSNKRVQSFRPVREEEVANLIADIEGLLSSASPTTVNLGERFAALTNDVVCRVAL 202

Query: 200 GAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFL 259
           G KYG G+   + +++L+EF+ELLG+    D+IPWL W+D +SGLD +  +VAKELD FL
Sbjct: 203 GRKYG-GK---RFKAILMEFVELLGALSFGDYIPWLAWVDRVSGLDERLDKVAKELDGFL 262

Query: 260 DRVIQDHVDSENRDENS----DKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAG 319
           D V+++HV+     E      +   D VD+LL IQ+++S+GF ++  +IKALILD F+AG
Sbjct: 263 DEVVEEHVEKRMNCEGGVDVQEGKEDFVDILLSIQKDSSVGFSIDKITIKALILDAFAAG 322

Query: 320 TDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQY-VNEDDLDKMHYLKAVMKETLRL 379
           TDTT T LEWAM+EL +HP AM++ ++E+R I+G +  + E+DLD+M YLKAV+KETLR 
Sbjct: 323 TDTTYTALEWAMTELLRHPNAMRQAQAEVRRIAGHKSSITEEDLDQMCYLKAVIKETLRS 382

Query: 380 HPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSID 439
           HPPIPLL+PRESTQ  K+ GYD+  +TRV++NAWAIGRDP +W+  +EFRPERF+NSSI+
Sbjct: 383 HPPIPLLIPRESTQDTKVMGYDVAARTRVIVNAWAIGRDPSLWDSPDEFRPERFLNSSIN 442

Query: 440 FKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG-EDLDMTGAFGLTI 499
           F+G DFELIPFGAGRRGCPG+ FA V++E+ LANL+HKF+W LP G   LDMT + GLTI
Sbjct: 443 FQGHDFELIPFGAGRRGCPGIAFAIVVDELVLANLLHKFDWRLPDGVGQLDMTESTGLTI 502

BLAST of CmaCh10G000890 vs. TrEMBL
Match: A0A059D144_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B01015 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 4.9e-161
Identity = 284/490 (57.96%), Postives = 376/490 (76.73%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           ++LF WLL    S++Q+    PPSPP +LPI G+L ++GS PH +L + +R+HGPL LL 
Sbjct: 23  LLLFKWLL--SPSTTQKHHHPPPSPP-RLPILGNLHQIGSRPHSSLANLSRKHGPLMLLR 82

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
           LG  P +VVSS++ AR++MKTHDL+FANRPKS I +KL+Y+  D+S APYGEYWRQM+SV
Sbjct: 83  LGRVPVLVVSSADAAREIMKTHDLIFANRPKSKIFEKLVYDYKDVSMAPYGEYWRQMKSV 142

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQ-----RNPISVNLTETFSGLTNNVVCRVAL 199
            VL LLSNKRVQSFR VREEEV  +I  I+      +P +VNL E F+ LTN+VVCRVAL
Sbjct: 143 FVLQLLSNKRVQSFRPVREEEVANLITDIEGLLSSASPTTVNLGERFAALTNDVVCRVAL 202

Query: 200 GAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFL 259
           G KYG G+   + +++L+EF+ELLG+    D+IPWL W+D +SGLD +  +VAKELD FL
Sbjct: 203 GRKYG-GK---RFKAILMEFVELLGALSFGDYIPWLAWVDRVSGLDERLDKVAKELDGFL 262

Query: 260 DRVIQDHVDSENRDENS----DKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAG 319
           D V+++HV+     E      +   D VD+LL IQ+++S+GF ++  +IKALILD F+AG
Sbjct: 263 DEVVEEHVEKRMNCEGGVDVQEGKEDFVDILLSIQKDSSVGFSIDKITIKALILDAFAAG 322

Query: 320 TDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQY-VNEDDLDKMHYLKAVMKETLRL 379
           TDTT T LEWAM+EL +HP AM++ ++E+R I+G +  + E+DLD+M YLKAV+KETLR 
Sbjct: 323 TDTTYTALEWAMTELLRHPNAMRQAQAEVRRIAGHKSSITEEDLDQMCYLKAVIKETLRS 382

Query: 380 HPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSID 439
           HPPIPLL+PRESTQ  K+ GYD+  +TRV++NAWAIGRDP +W+  +EFRPERF+NSSI+
Sbjct: 383 HPPIPLLIPRESTQDTKVMGYDVVARTRVIVNAWAIGRDPSLWDSPDEFRPERFLNSSIN 442

Query: 440 FKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG-EDLDMTGAFGLTI 499
           FKG DFELIPFGAGRRGCPG+ F+  ++E+ LANL+HKF+W LP G   LDMT + GLTI
Sbjct: 443 FKGHDFELIPFGAGRRGCPGIAFSIAVDELVLANLLHKFDWRLPDGVGQLDMTESTGLTI 502

BLAST of CmaCh10G000890 vs. TAIR10
Match: AT3G48280.1 (AT3G48280.1 cytochrome P450, family 71, subfamily A, polypeptide 25)

HSP 1 Score: 514.2 bits (1323), Expect = 8.9e-146
Identity = 253/486 (52.06%), Postives = 353/486 (72.63%), Query Frame = 1

Query: 21  VLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLHL 80
           ++F+ +LF +K  S ++  +PPSPP  LP+ G+L +LG H H +L   +RR+GPL LLHL
Sbjct: 11  IIFMTILFLKKQLSGKKGKTPPSPPG-LPLIGNLHQLGRHTHRSLCDLSRRYGPLMLLHL 70

Query: 81  GSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSVG 140
           G  P ++VSS+++A++++KTHD  FANRP+S +S KLLYN  D+++APYGEYWRQM+SV 
Sbjct: 71  GRVPVLIVSSADMAQEILKTHDQAFANRPRSKLSQKLLYNNRDVASAPYGEYWRQMKSVC 130

Query: 141 VLHLLSNKRVQSFRSVREEEVKLMIEKIQR-NPISVNLTETFSGLTNNVVCRVALGAKYG 200
           V+HLLSNK V+SFR VREEE+ LM+ KI++ + +  N+++    LTN+V+CRVALG KYG
Sbjct: 131 VIHLLSNKMVRSFRDVREEEITLMMAKIRKSSSLPFNVSKVLECLTNDVICRVALGRKYG 190

Query: 201 VGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVIQ 260
              D+ KL   L    ELLG+F +  F+PWL W+D I G DA+  ++ K+LD F ++V+Q
Sbjct: 191 GETDFKKLTDRL---SELLGTFSIGSFVPWLAWVDWIRGWDAQLDKMGKDLDDFFEKVVQ 250

Query: 261 DHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVLE 320
           DH D + RD       DL+D LL ++R  S GF +E  SIKA+ LD+F  G+DT+ T+LE
Sbjct: 251 DHEDGDRRDGT-----DLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLE 310

Query: 321 WAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLVP 380
           WAM+EL +HP+++ +L+ E+R I  G   V+EDD+  M YLKAV+KE LRLHPP P++ P
Sbjct: 311 WAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPMMAP 370

Query: 381 RESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFEL 440
            EST+ +KLR Y I   T+VM+NAWAIGR+   W  +AEEF+PER +++S+DF+GQ+FEL
Sbjct: 371 HESTEDVKLRDYHIPAGTQVMMNAWAIGREVATWGPDAEEFKPERHLDTSVDFRGQNFEL 430

Query: 441 IPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLP--SGED-LDMTGAFGLTIHRKHPL 500
           +PFGAGRR CP V FA V+NE+ LANLVH F+W LP  S ED  D+  + G ++HR+ PL
Sbjct: 431 LPFGAGRRICPAVSFAVVLNEVVLANLVHGFDWKLPEESKEDKTDVAESSGFSVHREFPL 487

BLAST of CmaCh10G000890 vs. TAIR10
Match: AT3G48270.1 (AT3G48270.1 cytochrome P450, family 71, subfamily A, polypeptide 26)

HSP 1 Score: 504.2 bits (1297), Expect = 9.2e-143
Identity = 251/486 (51.65%), Postives = 355/486 (73.05%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           I+  + ++  +K    +++++ PSPP  LP+ G+L +LG HPH +L S + R+GPL LLH
Sbjct: 11  IIFVVTIIIFRKQKRGKKRNTLPSPPG-LPLIGNLHQLGRHPHRSLCSLSHRYGPLMLLH 70

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
            G  P +VVSS+E+ARDV+KTHD VFA+RP+S I +KLLY+  D+++APYGEYWRQM+SV
Sbjct: 71  FGRVPVLVVSSAELARDVLKTHDRVFASRPRSKIFEKLLYDKHDVASAPYGEYWRQMKSV 130

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRN-PISVNLTETFSGLTNNVVCRVALGAKY 199
            VLHL SNK V+SFR VREEE+ LM+EKI+++  + VNL++    LTN+V+C+VALG KY
Sbjct: 131 CVLHLFSNKMVRSFREVREEEISLMMEKIRKSISLPVNLSKILVSLTNDVICKVALGRKY 190

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
           G   D+   + L+    +LLG+F V  ++PWL WID I GLD + ++ A ++D F +RV+
Sbjct: 191 GGETDF---KELMERLNKLLGTFSVGSYVPWLAWIDWIRGLDCQLEKTANDVDKFFERVV 250

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDHVD  NRD       D VDVLL IQR+ ++GF +   SIKA+++++F  GTDT+ST++
Sbjct: 251 QDHVDG-NRDMT-----DFVDVLLAIQRDKTVGFEINRVSIKAIVMNVFVGGTDTSSTLM 310

Query: 320 EWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           EWAM+EL +HP+ +K+L+ E+R I  D+  V+E+++  M YLKAV+KE LRLHPP+PL+V
Sbjct: 311 EWAMTELLRHPKCLKRLQEEVRTICKDKSSVSEEEIQNMSYLKAVIKEALRLHPPLPLMV 370

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P ESTQ ++L  + I   T+V+INAWAIGR+   W  + EEFRPER ++SS+D++GQ FE
Sbjct: 371 PHESTQDVRLGDHHIPAGTQVLINAWAIGREAATWGPDVEEFRPERHLDSSVDYRGQAFE 430

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWML--PSGED-LDMTGAFGLTIHRKHP 499
           LIPFG+GRR CP + FA V+NE+ LANLVH+F+W L   S ED  ++  + G+ IHR  P
Sbjct: 431 LIPFGSGRRICPAISFAVVLNEVVLANLVHRFDWRLSVESTEDQTEVAESTGIAIHRMFP 486

BLAST of CmaCh10G000890 vs. TAIR10
Match: AT3G48310.1 (AT3G48310.1 cytochrome P450, family 71, subfamily A, polypeptide 22)

HSP 1 Score: 501.9 bits (1291), Expect = 4.6e-142
Identity = 249/483 (51.55%), Postives = 344/483 (71.22%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           +++FI +LF  K    ++ ++P SPP +LP+ G+L +LG HPH +L S + R+GPL LL 
Sbjct: 12  LIIFITILFFIKQKKGKKSNTPASPP-RLPLIGNLHQLGRHPHRSLCSLSNRYGPLMLLR 71

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
            G  P +VVSS+++ARD++KT+D VFA+RP+S I +K+ Y A D++ APYGEYWRQM+SV
Sbjct: 72  FGLVPVLVVSSADVARDILKTYDRVFASRPRSKIFEKIFYEARDVALAPYGEYWRQMKSV 131

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNP-ISVNLTETFSGLTNNVVCRVALGAKY 199
            VLHLL+NK V+SFR+VR+EE+ LM+EKIQ++  + VNL+E    LTN+V+ RVALG KY
Sbjct: 132 CVLHLLTNKMVRSFRNVRQEEISLMMEKIQKSSSLQVNLSELLGSLTNDVISRVALGRKY 191

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
               D+   + L+    +LLG F V  ++PWL WID ISGLD + K+   +LD FL++V+
Sbjct: 192 SDETDF---KELMKRLTKLLGEFCVGTYVPWLAWIDWISGLDGQLKKTGNDLDEFLEKVV 251

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDH D + +        D VDVLL IQR  S+GF ++  SIKA+ILD+   GTDT+  ++
Sbjct: 252 QDHEDGDAQ------RTDFVDVLLRIQREKSVGFEIDRLSIKAIILDVVVGGTDTSYALM 311

Query: 320 EWAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           EWAM+EL   PE + +L+ E+R I  G+  V+EDD+  M+YLKAV+KET+RLHPP+PL+V
Sbjct: 312 EWAMTELLHRPECLNRLQEEVRTICKGNSSVSEDDIKDMNYLKAVIKETMRLHPPLPLMV 371

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P ESTQ ++L  Y I   T+VMINAWAIGR+   W  +AE+FRPER +NSS+DF+G +FE
Sbjct: 372 PHESTQDVRLGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLNSSVDFRGHNFE 431

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTG---AFGLTIHRKHP 497
           LIPFGAGRR CP + FA ++ E+ LANLVH+++W LP     D T    + G+ IHR  P
Sbjct: 432 LIPFGAGRRICPAISFAVILIEVTLANLVHRYDWRLPEEYIEDQTNVAESTGMVIHRLFP 484

BLAST of CmaCh10G000890 vs. TAIR10
Match: AT3G48320.1 (AT3G48320.1 cytochrome P450, family 71, subfamily A, polypeptide 21)

HSP 1 Score: 496.5 bits (1277), Expect = 1.9e-140
Identity = 243/486 (50.00%), Postives = 343/486 (70.58%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           +++FI +LF +K    ++ ++P SPP +LP+ G+L +LG HPH +L S + R+GPL LLH
Sbjct: 12  LIIFITILFFKKQKRGKKSNTPRSPP-RLPLIGNLHQLGHHPHRSLCSLSHRYGPLMLLH 71

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
           LG  P +VVSS+++ARD++KTHD VFA+RP+S + +KL Y+  D++ APYGEYWRQ++SV
Sbjct: 72  LGRVPVLVVSSADVARDILKTHDRVFASRPRSKLFEKLFYDGRDVAFAPYGEYWRQIKSV 131

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQR-NPISVNLTETFSGLTNNVVCRVALGAKY 199
            VL LLSNK V SFR+VR+EE+ LM+EKIQ+ + + VN++E    LTN+V+ R+ALG KY
Sbjct: 132 CVLRLLSNKMVTSFRNVRQEEISLMMEKIQKSSSLQVNVSELLGSLTNDVISRIALGRKY 191

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
               D    + L+   + L+G F V  ++PWLGWID ISGLD +  +   +LD FL++V+
Sbjct: 192 SGETD---SKELMKRLMMLMGEFSVGTYVPWLGWIDWISGLDGQLNKTGNDLDEFLEKVV 251

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDHVD +       +  D VDVLL IQR  SIGF ++   IKA++LD+  AGTD++  ++
Sbjct: 252 QDHVDGDG------QRTDFVDVLLRIQREKSIGFEIDRLCIKAIVLDVLVAGTDSSYALM 311

Query: 320 EWAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           +WAM+EL +HPE ++ L+ E+R I  G+  V+E+D+  M YLKAV+KET RLHPP+PLL 
Sbjct: 312 DWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLHPPLPLLA 371

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P ES Q + L  Y I   T+VMINAWAIGR+   W  +AE+FRPER ++SS+DF+G +FE
Sbjct: 372 PHESIQDVILGDYHIPAGTQVMINAWAIGREAATWGPDAEKFRPERHLDSSVDFRGHNFE 431

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTG---AFGLTIHRKHP 499
           L+PFGAGRR CP + FA V+ E+ LAN VH+++W LP     + T    + G+ IHR  P
Sbjct: 432 LVPFGAGRRICPAISFAVVLIEVALANFVHRYDWKLPEDSKENQTNVAESTGMVIHRLFP 487

BLAST of CmaCh10G000890 vs. TAIR10
Match: AT3G48300.1 (AT3G48300.1 cytochrome P450, family 71, subfamily A, polypeptide 23)

HSP 1 Score: 495.4 bits (1274), Expect = 4.3e-140
Identity = 246/478 (51.46%), Postives = 338/478 (70.71%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           I+  I +LF +K  +  +  + PSPP +LP+ G+L +L  HPH +L   + R+GPL LLH
Sbjct: 9   ILFIITILFFKKHKTVNKIINFPSPP-RLPLIGNLHQLSQHPHRSLCYLSHRYGPLMLLH 68

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
            GS P IV S++E ARDV+KTHD VFA+RP+S I +KLLY + ++++APYGEYWRQM+SV
Sbjct: 69  FGSVPVIVASTAEAARDVLKTHDRVFASRPRSKIFEKLLYKSRNMASAPYGEYWRQMKSV 128

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPIS-VNLTETFSGLTNNVVCRVALGAKY 199
            VLHLLSNK V+SF+ VR+EE+ LM+E I+++    VNL++  S LTN+V+CRVALG KY
Sbjct: 129 SVLHLLSNKMVRSFQDVRQEEITLMMETIRKSSSKPVNLSKILSSLTNDVICRVALGRKY 188

Query: 200 GVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDRVI 259
           GVG D+   + L+   +  LG+F +  ++PWL W D +SGL+A+ ++ A + D  L+R++
Sbjct: 189 GVGTDF---KELIDRLMRQLGTFTIGSYVPWLAWTDWVSGLEARLEKTANDFDKLLERIV 248

Query: 260 QDHVDSENRDENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTTSTVL 319
           QDH D +      DK  D VDVLL  QR+ S GF ++  SIKA++LD F  GTDT+ST++
Sbjct: 249 QDHEDGD-----GDK-TDFVDVLLAAQRDKSFGFDIDRLSIKAIVLDAFVGGTDTSSTLV 308

Query: 320 EWAMSELFKHPEAMKKLKSEIREI-SGDQYVNEDDLDKMHYLKAVMKETLRLHPPIPLLV 379
           EW M+EL +HP  +KKL+ E+R I  G   V+EDD+  M YLKAV+KE LRLHPP+PL+V
Sbjct: 309 EWEMTELLRHPTCLKKLQEEVRTICKGKSSVSEDDIQGMEYLKAVVKEALRLHPPVPLMV 368

Query: 380 PRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW-EEAEEFRPERFMNSSIDFKGQDFE 439
           P +STQ ++LR   I   T+V++N WA+GR+   W  +A EFRPER + S  DF+GQDFE
Sbjct: 369 PHQSTQDVRLRDNHIPAGTQVIVNLWAVGREAATWGPDANEFRPERHLESPSDFRGQDFE 428

Query: 440 LIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTGAFGLTIHRKHPL 495
           LIPFGAGRR CPG+ FA V+NE+ LANLVH F+W     ++ D+  + G  I R HPL
Sbjct: 429 LIPFGAGRRMCPGISFAVVLNEVVLANLVHGFDWQ-SIDDETDVAESIGSVIRRMHPL 475

BLAST of CmaCh10G000890 vs. NCBI nr
Match: gi|449440634|ref|XP_004138089.1| (PREDICTED: cytochrome P450 71A22-like [Cucumis sativus])

HSP 1 Score: 770.4 bits (1988), Expect = 1.9e-219
Identity = 379/494 (76.72%), Postives = 426/494 (86.23%), Query Frame = 1

Query: 12  HPVSVAGFIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARR 71
           + +S   FI+L I+LL  QK      QSSPPSPP K+PIFGHLL LGS PH+TLQ+YAR 
Sbjct: 18  YSISFTAFILLLIFLL--QKRLLSSSQSSPPSPPAKIPIFGHLLSLGSLPHLTLQNYARL 77

Query: 72  HGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGE 131
           HGPLFLL LGS PT+VVSSSE+ARD+MKTHDL+FANRPKSSISDKLLY + D++A+PYGE
Sbjct: 78  HGPLFLLRLGSVPTLVVSSSELARDIMKTHDLIFANRPKSSISDKLLYGSRDVAASPYGE 137

Query: 132 YWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTETFSGLTNNVVCR 191
           YWRQM+SV VLH+LSNKRVQSFR VREEEVKLMIEKI++NP+ VNLTE  SGLTN+VVCR
Sbjct: 138 YWRQMKSVCVLHMLSNKRVQSFRCVREEEVKLMIEKIEQNPVGVNLTEILSGLTNDVVCR 197

Query: 192 VALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELD 251
           V LG KY VGED +K  SLL +F ELLGSF VRDFIPWLGWID ISGLD KA R+AKELD
Sbjct: 198 VGLGRKYRVGEDGVKFMSLLKKFGELLGSFSVRDFIPWLGWIDWISGLDGKANRIAKELD 257

Query: 252 VFLDRVIQDHVDSENRD-ENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAG 311
            F DRVI+DH++ EN++  N D+  DLVDVLLWIQR NSIGFPLEM+SIKALILDMF+AG
Sbjct: 258 EFFDRVIEDHMNPENKEMRNFDEQKDLVDVLLWIQRENSIGFPLEMESIKALILDMFAAG 317

Query: 312 TDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGD---QYVNEDDLDKMHYLKAVMKETL 371
           TDTT TVLEWAMSEL KHPE MKKLK+EIREI G+    YVNEDDL+KM YLKAV KETL
Sbjct: 318 TDTTYTVLEWAMSELLKHPEVMKKLKNEIREIKGEHKGSYVNEDDLNKMVYLKAVFKETL 377

Query: 372 RLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSS 431
           RLH PIPLLVPRES + +KL GYDIKP TRVMINAW IGRDP+VWEEAE+F+PERFMNSS
Sbjct: 378 RLHTPIPLLVPRESIKPVKLGGYDIKPGTRVMINAWTIGRDPKVWEEAEKFQPERFMNSS 437

Query: 432 IDFKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTGAFGLT 491
           IDFKGQDFELIPFGAGRRGCPG+ FA +++EI LANLVHKFEW+LP+GEDLDMTGAFGL+
Sbjct: 438 IDFKGQDFELIPFGAGRRGCPGIMFAAMVSEITLANLVHKFEWILPNGEDLDMTGAFGLS 497

Query: 492 IHRKHPLVATATPC 502
           IHRK PLVATA PC
Sbjct: 498 IHRKFPLVATAIPC 509

BLAST of CmaCh10G000890 vs. NCBI nr
Match: gi|659129069|ref|XP_008464513.1| (PREDICTED: cytochrome P450 71A22-like [Cucumis melo])

HSP 1 Score: 756.9 bits (1953), Expect = 2.2e-215
Identity = 375/495 (75.76%), Postives = 424/495 (85.66%), Query Frame = 1

Query: 12  HPVSVAGFIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARR 71
           + +S   FI+L I+LL  QK      QSSPPSPP KLPIFGHLL LGS PH+TLQ+YAR 
Sbjct: 19  YSISFTTFILLLIFLL--QKCLRSSPQSSPPSPP-KLPIFGHLLNLGSLPHLTLQNYARL 78

Query: 72  HGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGE 131
           HGPLFLL LGS PT+VVS+S++ARD+MKTHDLVFANRPKSSISDKLLY++ D++A+PYGE
Sbjct: 79  HGPLFLLRLGSVPTLVVSASDLARDIMKTHDLVFANRPKSSISDKLLYSSRDVAASPYGE 138

Query: 132 YWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPISVNLTETFSGLTNNVVCR 191
           YWRQM+S+ VLHLLSNKRVQSFR VREEEVKLMIEKI++NP+ VNLTE FSGLTN+VVCR
Sbjct: 139 YWRQMKSICVLHLLSNKRVQSFRHVREEEVKLMIEKIEQNPVGVNLTEIFSGLTNDVVCR 198

Query: 192 VALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELD 251
           VALG KY VGED +K   LL EF +LLGSF VRDFIPWLGWID ISGLD KA  VAKELD
Sbjct: 199 VALGRKYRVGEDGVKFMGLLKEFSKLLGSFSVRDFIPWLGWIDWISGLDGKANGVAKELD 258

Query: 252 VFLDRVIQDHVDSENRDE--NSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSA 311
            F DRVI+DH++ ENR +  N D+  DLVDVLLWIQR NSIGFPLEM +IKALILDMF+A
Sbjct: 259 KFFDRVIEDHMNPENRADMKNFDEQKDLVDVLLWIQRENSIGFPLEMKNIKALILDMFAA 318

Query: 312 GTDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGD---QYVNEDDLDKMHYLKAVMKET 371
           GTDT  TVLEWAMSEL KHPE MKKLK+EI EI G+    Y+NEDD+D+M YLKA++KET
Sbjct: 319 GTDTAYTVLEWAMSELLKHPEVMKKLKNEIGEIKGEHKGSYINEDDIDQMVYLKAIIKET 378

Query: 372 LRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNS 431
           LRLH PIPLLVPRES + +KLRGYDIKP TRVMINAW IGRDP+VWEEAE+F+PERF+NS
Sbjct: 379 LRLHTPIPLLVPRESIKPVKLRGYDIKPGTRVMINAWTIGRDPKVWEEAEKFQPERFLNS 438

Query: 432 SIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSGEDLDMTGAFGL 491
           SIDFKGQDFELIPFGAGRRGCPG+ FAT + EI LANLVHKFEW+LP+GEDLDM+GA GL
Sbjct: 439 SIDFKGQDFELIPFGAGRRGCPGIAFATTVIEIALANLVHKFEWILPNGEDLDMSGASGL 498

Query: 492 TIHRKHPLVATATPC 502
           TIHRK PLVATA PC
Sbjct: 499 TIHRKIPLVATAVPC 510

BLAST of CmaCh10G000890 vs. NCBI nr
Match: gi|590669464|ref|XP_007037781.1| (Cytochrome P450, family 71, subfamily A, polypeptide 25, putative [Theobroma cacao])

HSP 1 Score: 608.2 bits (1567), Expect = 1.3e-170
Identity = 307/511 (60.08%), Postives = 382/511 (74.76%), Query Frame = 1

Query: 7   VALIGHPVSVAGFIVLFIWLLFR--QKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHIT 66
           +  + +P   A   + F+ LL      S+S  + + PPSP +K PIFG+L  +G  PH +
Sbjct: 13  IFFLSYPWLFAILAIFFVVLLKHIFNPSTSNAKLALPPSP-SKFPIFGNLHNIGLQPHRS 72

Query: 67  LQSYARRHGPLFLLHLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDI 126
           L++ A+R GPL LLHLGS P +VVSSS+ AR++MKTHDL FANRPKSS S KLLY+  D+
Sbjct: 73  LRALAQRFGPLMLLHLGSVPVLVVSSSDAAREIMKTHDLTFANRPKSSSSQKLLYDYKDV 132

Query: 127 SAAPYGEYWRQMRSVGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPIS---VNLTETF 186
           S+APYGEYWRQM+S  VLH LSNKRVQSFR VREEE  L+I+KI+ +  S   VNLTE F
Sbjct: 133 SSAPYGEYWRQMKSACVLHFLSNKRVQSFRVVREEETALIIDKIKESSSSALPVNLTEIF 192

Query: 187 SGLTNNVVCRVALGAKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDA 246
           + LTNNV+CR ALG KY  GE   K + LL EF+ELLG F V D+IPWL W+  I+GL+A
Sbjct: 193 AMLTNNVICRAALGRKYSEGEGGKKFKKLLCEFVELLGGFNVGDYIPWLAWVSRINGLEA 252

Query: 247 KAKRVAKELDVFLDRVIQDHVDSENRDENS--------DKHMDLVDVLLWIQRNNSIGFP 306
           K ++VAKE D FL+ V+ +H+D   R  N         ++    VDVLL IQR N++GFP
Sbjct: 253 KMEKVAKEFDAFLEGVVDEHMDRYKRGHNDHDDTRLQHEEQKSFVDVLLEIQRENTVGFP 312

Query: 307 LEMDSIKALILDMFSAGTDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDD 366
           LE  S+KALILDMF+AGTDTT TVLEWAM+EL +HPE MK+L+ E+R++ GD+ YV+EDD
Sbjct: 313 LERVSVKALILDMFAAGTDTTYTVLEWAMTELLRHPEIMKELQKEVRQVCGDETYVSEDD 372

Query: 367 LDKMHYLKAVMKETLRLHPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVW 426
           LDKMHYLKAV+KE+LRLHPPIPLLVPR ST+ +K++GYDI   T+V+INAWAIGRDP  W
Sbjct: 373 LDKMHYLKAVIKESLRLHPPIPLLVPRISTEDVKIKGYDIARGTQVIINAWAIGRDPLSW 432

Query: 427 EEAEEFRPERFMNSSIDFKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWML 486
            + EEF PER +N+SIDFKG DFELIPFG+GRR CPG+ FA  INE+ LANLVHKF+W L
Sbjct: 433 AKPEEFLPERILNNSIDFKGHDFELIPFGSGRRICPGILFAMKINELFLANLVHKFDWSL 492

Query: 487 PSG---EDLDMTGAFGLTIHRKHPLVATATP 501
           P G   EDLDM  +FGLT HR++PL+A A P
Sbjct: 493 PGGAKEEDLDMGESFGLTTHRRYPLIAIANP 522

BLAST of CmaCh10G000890 vs. NCBI nr
Match: gi|502176358|ref|XP_004515856.1| (PREDICTED: cytochrome P450 71A26-like [Cicer arietinum])

HSP 1 Score: 585.9 bits (1509), Expect = 6.8e-164
Identity = 297/495 (60.00%), Postives = 376/495 (75.96%), Query Frame = 1

Query: 20  IVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLLH 79
           I L I+L+ +  S+S++ ++S PSPP KLPI G+L +LGS PH TLQS ++++GPL  LH
Sbjct: 8   ISLIIFLIIKWYSNSRKIKNSLPSPP-KLPIIGNLHQLGSFPHRTLQSLSKKYGPLMKLH 67

Query: 80  LGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRSV 139
           LGS P IV+SS E ARD+MKTHDLVFANRP+S   D LLY+  D+SAAPYGEYWRQ+RS+
Sbjct: 68  LGSVPVIVISSPESARDIMKTHDLVFANRPQSKFYDILLYDCKDVSAAPYGEYWRQIRSI 127

Query: 140 GVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNPIS---VNLTETFSGLTNNVVCRVALGA 199
            VLHLLS KRVQS R VREEEV LMIEKI+++  S   +N++E  S   N++VCRVALG 
Sbjct: 128 SVLHLLSGKRVQSLRGVREEEVCLMIEKIKQSSSSSLPINISELLSTTINDIVCRVALGR 187

Query: 200 KYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLDR 259
           KY  GE     + LL++F ELLG+F V +++PWL W+  +SGL A+AK+VAKE D  L+ 
Sbjct: 188 KYS-GESGKGFKKLLMDFTELLGTFVVGEYVPWLDWLTHVSGLYARAKKVAKEFDELLED 247

Query: 260 VIQDHVDSE--NRDE----NSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAG 319
           V++DH++    +RDE    +S+ H D VDVLL IQR  S+GFP++   IKAL+LDMFSAG
Sbjct: 248 VVEDHINRNKGDRDERVCLDSEDHGDFVDVLLSIQRTESLGFPIDRTVIKALLLDMFSAG 307

Query: 320 TDTTSTVLEWAMSELFKHPEAMKKLKSEIREISGDQ-YVNEDDLDKMHYLKAVMKETLRL 379
           TDT ST+LEWAM+EL +HP  MKKL+ E R + GD+ ++ EDDL  M YLKAV+KETLRL
Sbjct: 308 TDTISTLLEWAMTELLRHPNVMKKLQEEGRTVGGDRTHITEDDLSNMKYLKAVIKETLRL 367

Query: 380 HPPIPLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSID 439
           HPPIPLLVPRES + IKL+GY I+  TRV+INAWAI RDP  W++ EEF+PERF+N+SID
Sbjct: 368 HPPIPLLVPRESRKDIKLKGYHIERGTRVIINAWAIARDPSYWDQPEEFKPERFLNNSID 427

Query: 440 FKGQDFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG----EDLDMTGAFG 499
            KG DF+LIPFGAGRRGCPG+ +A V NE+ LANLVH+F W LP+G    E +DM+   G
Sbjct: 428 IKGNDFQLIPFGAGRRGCPGIVYAMVANELVLANLVHQFNWTLPAGVLGLEAIDMSETVG 487

Query: 500 LTIHRKHPLVATATP 501
           LT+HRK PLVA   P
Sbjct: 488 LTMHRKTPLVAVVVP 500

BLAST of CmaCh10G000890 vs. NCBI nr
Match: gi|731424959|ref|XP_010663080.1| (PREDICTED: cytochrome P450 71A25 [Vitis vinifera])

HSP 1 Score: 581.3 bits (1497), Expect = 1.7e-162
Identity = 290/491 (59.06%), Postives = 370/491 (75.36%), Query Frame = 1

Query: 19  FIVLFIWLLFRQKSSSQEEQSSPPSPPTKLPIFGHLLKLGSHPHITLQSYARRHGPLFLL 78
           FIVL +  LF    ++ +  + PPSPP +LP+ G++ +LG +P+ +L   AR +GPL LL
Sbjct: 27  FIVLLVRWLFSTPPTTHK--TLPPSPP-RLPVLGNMHQLGIYPYRSLLCLARCYGPLMLL 86

Query: 79  HLGSKPTIVVSSSEIARDVMKTHDLVFANRPKSSISDKLLYNASDISAAPYGEYWRQMRS 138
            LG   T+VVSS + A+++MKTHDL+FANRPK S+  +LLY+  D+S APYGEYWRQMRS
Sbjct: 87  QLGRVRTLVVSSPDAAQEIMKTHDLIFANRPKMSLGKRLLYDYKDVSVAPYGEYWRQMRS 146

Query: 139 VGVLHLLSNKRVQSFRSVREEEVKLMIEKIQRNP---ISVNLTETFSGLTNNVVCRVALG 198
           + VLHLLSNKRVQSF +VR EE+ L+I+KI+       S++L+  F  LTN+V+CRVA G
Sbjct: 147 ICVLHLLSNKRVQSFNTVRREEISLLIQKIEEFSSLSTSMDLSGMFMRLTNDVICRVAFG 206

Query: 199 AKYGVGEDWIKLRSLLLEFLELLGSFGVRDFIPWLGWIDGISGLDAKAKRVAKELDVFLD 258
            KY   E   K R LL EF+ELLG F V D+IPWL W++ ++G  AK +RVAKE D FLD
Sbjct: 207 RKYSGDERGKKFRRLLGEFVELLGGFNVGDYIPWLAWVEYVNGWSAKVERVAKEFDEFLD 266

Query: 259 RVIQDHVDSENRD-ENSDKHMDLVDVLLWIQRNNSIGFPLEMDSIKALILDMFSAGTDTT 318
            V+++H+D         D   D VDVLL IQR+ ++GF ++ DSIKALILD+F+ GTDTT
Sbjct: 267 GVVEEHLDGGTGSIAKGDNEKDFVDVLLEIQRDGTLGFSMDRDSIKALILDIFAGGTDTT 326

Query: 319 STVLEWAMSELFKHPEAMKKLKSEIREIS-GDQYVNEDDLDKMHYLKAVMKETLRLHPPI 378
            TVLEWAM+EL +HP+AMK+L++E+R I+ G +++ EDDL+KMHYLKAV+KETLRLHPPI
Sbjct: 327 YTVLEWAMTELLRHPKAMKELQNEVRGITRGKEHITEDDLEKMHYLKAVIKETLRLHPPI 386

Query: 379 PLLVPRESTQCIKLRGYDIKPKTRVMINAWAIGRDPEVWEEAEEFRPERFMNSSIDFKGQ 438
           PLLVPRES+Q + + GY I   T V+INAWAIGRDP  W+E EEFRPERF+N++IDFKG 
Sbjct: 387 PLLVPRESSQDVNIMGYHIPAGTMVIINAWAIGRDPMSWDEPEEFRPERFLNTNIDFKGH 446

Query: 439 DFELIPFGAGRRGCPGVEFATVINEIGLANLVHKFEWMLPSG---EDLDMTGAFGLTIHR 498
           DFELIPFGAGRRGCPG+ FA   NE+ LANLV+KF+W LP G   EDLDMT   GLTIHR
Sbjct: 447 DFELIPFGAGRRGCPGISFAMATNELVLANLVNKFDWALPDGARAEDLDMTECTGLTIHR 506

Query: 499 KHPLVATATPC 502
           K PL+A +TPC
Sbjct: 507 KFPLLAVSTPC 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C71AP_ARATH1.6e-14452.06Cytochrome P450 71A25 OS=Arabidopsis thaliana GN=CYP71A25 PE=2 SV=1[more]
C71AO_ARATH6.0e-14452.78Cytochrome P450 71A24 OS=Arabidopsis thaliana GN=CYP71A24 PE=2 SV=3[more]
C71A4_SOLME3.0e-14352.85Cytochrome P450 71A4 OS=Solanum melongena GN=CYP71A4 PE=2 SV=1[more]
C71AQ_ARATH1.6e-14151.65Cytochrome P450 71A26 OS=Arabidopsis thaliana GN=CYP71A26 PE=3 SV=1[more]
C71AM_ARATH8.1e-14151.55Cytochrome P450 71A22 OS=Arabidopsis thaliana GN=CYP71A22 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A061FZH7_THECC8.9e-17160.08Cytochrome P450, family 71, subfamily A, polypeptide 25, putative OS=Theobroma c... [more]
A5B9L0_VITVI3.4e-16258.86Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_035274 PE=3 SV=1[more]
A0A059CXR3_EUCGR2.2e-16158.16Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B00204 PE=3 SV=1[more]
A0A059CZ64_EUCGR2.2e-16158.16Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B00199 PE=3 SV=1[more]
A0A059D144_EUCGR4.9e-16157.96Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_B01015 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48280.18.9e-14652.06 cytochrome P450, family 71, subfamily A, polypeptide 25[more]
AT3G48270.19.2e-14351.65 cytochrome P450, family 71, subfamily A, polypeptide 26[more]
AT3G48310.14.6e-14251.55 cytochrome P450, family 71, subfamily A, polypeptide 22[more]
AT3G48320.11.9e-14050.00 cytochrome P450, family 71, subfamily A, polypeptide 21[more]
AT3G48300.14.3e-14051.46 cytochrome P450, family 71, subfamily A, polypeptide 23[more]
Match NameE-valueIdentityDescription
gi|449440634|ref|XP_004138089.1|1.9e-21976.72PREDICTED: cytochrome P450 71A22-like [Cucumis sativus][more]
gi|659129069|ref|XP_008464513.1|2.2e-21575.76PREDICTED: cytochrome P450 71A22-like [Cucumis melo][more]
gi|590669464|ref|XP_007037781.1|1.3e-17060.08Cytochrome P450, family 71, subfamily A, polypeptide 25, putative [Theobroma cac... [more]
gi|502176358|ref|XP_004515856.1|6.8e-16460.00PREDICTED: cytochrome P450 71A26-like [Cicer arietinum][more]
gi|731424959|ref|XP_010663080.1|1.7e-16259.06PREDICTED: cytochrome P450 71A25 [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0016310 phosphorylation
biological_process GO:0044550 secondary metabolite biosynthetic process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0016301 kinase activity
molecular_function GO:0097159 organic cyclic compound binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G000890.1CmaCh10G000890.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 438..447
score: 6.4E-12coord: 361..372
score: 6.4E-12coord: 309..326
score: 6.4
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 24..500
score: 3.9E
IPR001128Cytochrome P450PFAMPF00067p450coord: 42..483
score: 2.9E
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 41..500
score: 8.51E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 69..88
score: 5.1E-52coord: 93..114
score: 5.1E-52coord: 184..202
score: 5.1E-52coord: 401..425
score: 5.1E-52coord: 447..470
score: 5.1E-52coord: 298..315
score: 5.1E-52coord: 360..378
score: 5.1E-52coord: 318..344
score: 5.1E-52coord: 437..447
score: 5.1
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 440..449
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 2..501
score: 3.8E
NoneNo IPR availablePANTHERPTHR24298:SF117SUBFAMILY NOT NAMEDcoord: 2..501
score: 3.8E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh10G000890CmaCh13G007640Cucurbita maxima (Rimu)cmacmaB092
CmaCh10G000890CmaCh18G002000Cucurbita maxima (Rimu)cmacmaB102