CmaCh08G013100 (gene) Cucurbita maxima (Rimu)

NameCmaCh08G013100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionCytochrome P450 family protein
LocationCma_Chr08 : 7913743 .. 7917158 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAATTAATTAATATATAATGATGGATGGCGTTGCTTCGTTTGCAGGCATTGGATTGGAGCAGGAATGAGAATGTGGGAGGCCTCACCCTGGTCCCTGCTGTGCGTTCTTACAGGGCTGTACGCGCTTGTGTTGGTTGCAAGAACACGAAGAAAACGAAAGGCGAGAGCATACAGGTTTCCTCCAGGGAGGAGAAGCTGGCCTCTAATAGGCGATAGCTTGAAGTGGTACAGTGCGGTGGCAAGCTCTCATCCGCCCAAGTATGTTGAAGAACAGGCCAAAAGGTACGGGAAGATCTTCTCGTGCCGTGTGTTCGGGAAATGGGCAGTGGTGTCGGTGGATCCCGTGTTTAACCGATACGTCATGCAAAACGAAGGCAAGTTGTTCAAATCCAGCTACCCGAAATCTTTCAGAGATTTGGTAGGGACCAACGGTGTGATCACAGCACAAGGGGAACAACAAAGGAAACTCCATGCAATTGCCTCCAATATGATGCGCCTACATGACCCCAAATTTCAGTTCCTGCGAGATATTCAAATCATAATCATTCAAACCTTGGGAAGTTTCCATAACGGCCAACTCATATGTCTCCAAGACGTTTGCAGGAAGGCTAGTAAATTCTTTTCTTTTGTTCACTATGGTTGGGATGGGAGTCAGTCTCGTAGGAATACATTTTCATTGGTATAAGGGGCTTTGAGAAAGTCCAAAGCAAAATCAGCCCTGAGAAATTATGCTCAAAATGAACCATATCATATCATGGAGAGTCTTAATTTCTAACATCTTTTTTTGCAAATTTTCTCCGATTACAATGGATTTATTTATTTATTTATTTATTTTTGTGTGTCACAGATAGCTATTAATCTAATGGTAAGTCAGGTACTAGGAGTGTCAAGTGAATCAGAAGTGGAAGAAATTAGTGGGTTATTTTACGACTTCGTGGATGGCTGTTTGTCTATTCCTATCAACTTTCCTGGCTTTGCCTATTACAGGGCCATGAAGGTAATATTTGACCAATATCGTGTAATCATTTATTATTCTTTCATTTTATTATTTTTTCATATTATTTATAATTTAGCATTATTTGTAATTTACATTTTGGTTACTGAATCATGGTTTGGCTACTTATTAACGCAGCTGTGATGTGTGATGTGTGTGGCATAGGCGAGGGAGCAAATCATAGGCAAAATTAAGAAGAAAATAGCGTGGCATAAGGCGAGGATGGCAGAGGGAAGGAGCGGGGAGGGAATGTTGGGAGGAGTATTGGAGGACCAGAAGTTGTCGGATGAGGCAGTTGCTGATTTCATTATCAATCTTCTTTTTGCTGGGAATGAAACCACATCTAAAACAATGCTTTTTGCAGTCTATTTCCTAACCCACTCACCCACAGCAATGAAGCAACTTGTGGTACCTTTCCCTATTTATACGCTAAATCTATGCTCCTTGGACTATTATTAATTAATTCCTTCTCTGTCTTTCTTTGTTTACATCAATTTATAAATTATAGGATGAGCAAGAGGGTTTAACAAACAAGAATGGAGATCATACAATCACATGGCAGGATTACAAATCGATGCCCTTTACTCAATGTGTAAGTTCCGGTTACGAATCACGATTCTACACAATACTATATCCGTAATATTAAAAGCCTCAATATAGGTTATTGACGAGACACTTCGACTGGGAGGAATTGCAATATGGTTGATGAGGGAGGCAAAAGAAGAGATCAAGTATTCAGGTTTTTTTTTTTTTTTTTTTTAATGATAATAATATAGTTCCAATAACGAAAAATGAATTTTTGGTTTGCATCGTTGATAGATTACGTTATTCCGAAAGGATGTTTCGTTGTTCCATTTCTTTCTGCTGTACATTTGGACGAGACTATATATGGAGAAGCTTCAACTTTCAACCCTTGGAGATGGATGGAGCTTAAGAATGAGGTTAGTCGGTTCAAAACCATGAGATTTTATGTTCAAAGTGGACAATATCTTACCATTATGAAGATCCATGAGAGTCATGTCTTTAATTTAGTCGTGTCAATAGAATCCTCAAATATCGAACAAAGAAGTTGTGAGACTCGAAGGTATAGTCAAAAGTGACTTTAGTTTTAAACAAAGTTTTAAACAAAGGGTGTACTTTGTTCGAGAACTCTAGAGAAGGAGTCGAGTCTTGATTAAGGGGAGGCTGTTCGAGAGATGTATTTTGTTCGAGGGGAGGATTGTTGGGAGAAATACATCTCCATTGGGATGAGGCATTTTGCGGAAGTCCAAAGCAAAACTACAAGAGTTTATGCTCAAAGTGGACAATATCATTCTATTGTGGATGAGTCGTGATTTCTAACATTTTAAGTCTTTGTTTTCCAAAGCGTTAGAGGCTAAGAATGATTAAAATTGTGGTTAATTATTAGGAAATGAGAAACTGGAGGAGTAGCCCATTCTTTGCTCCATTCGGGGGAGGTGCTAGATTATGTCCGGGAGCAGAATTGGCGCGCCTCCAAATTGCTCTATTTCTCCACCACTTTGTTACCAAGTATAGGTGAGTTGTTAAGGATATTTAATACTGAATTATAGTTTACCATACAACTAAATATTTAAACGGGTGGTTTTAGCCAATATTCTCACATTTAATTTTAAATTCTCTTCTCAGGTGGACCCAAGTTAGAGTAGATAGGATGTCTTTTTTTCCCTCTGCTCGCCTAGTCAACGGCTTCCAAATTCGCTTGAACACATCCCAACACTGATATATGACTTTCATAATGTTTCTTCCTTCGTTTTCTACTTCACTTATTTCTTGCCTAAATGAGTGTAGCTGAATATGTGCACATTTGTATTATAATGTTCAAGATTATGATGATGATTATGATTTTAAGTACTATTAGATAGTTGTTAATCAATCTCTAATAGTCGATTGTCTCAATTGACAGAATGTGTATATGAGTTAGAGTTACGACGAGGTCACAGATATGTATCATTTGAAAAACGAGTGTAATAATAGTAAAAGGTTTATAAAGTATCCATTTTTATTGAAAGAATTTGACATATTTTTTTAAGTTTAAAATAGATTGCAACCATTTTTGTAAGATTATATATTATTACAGCGTTGAAACCAATAAAGTATCCATTTTTATGTTTATTATATAAGTTAGAAGGAAAAAATAAAATATAAATAAGGATAAATTATATTTAATTAATTAATCAATCGACTCCCAGACCTGATTTTCATTTCTGTTGAATCTCCATTTTGTGAAGGCGAGGTCTTCCACTTCCAGCTTCGTTTTCATTGTGAATTGCTCTCTGCGTTTTTCGCCATTCCTTCCCTTTCACTCTACCCACCTAACTCCCACTCCCACTCCCGCTAGGGTTCCTTCGGGTTTTTCCCTCTCCAAATGTTCCTTTACTTGGATTCCATGCTACGCGTAG

mRNA sequence

TATAATTAATTAATATATAATGATGGATGGCGTTGCTTCGTTTGCAGGCATTGGATTGGAGCAGGAATGAGAATGTGGGAGGCCTCACCCTGGTCCCTGCTGTGCGTTCTTACAGGGCTGTACGCGCTTGTGTTGGTTGCAAGAACACGAAGAAAACGAAAGGCGAGAGCATACAGGTTTCCTCCAGGGAGGAGAAGCTGGCCTCTAATAGGCGATAGCTTGAAGTGGTACAGTGCGGTGGCAAGCTCTCATCCGCCCAAGTATGTTGAAGAACAGGCCAAAAGGTACGGGAAGATCTTCTCGTGCCGTGTGTTCGGGAAATGGGCAGTGGTGTCGGTGGATCCCGTGTTTAACCGATACGTCATGCAAAACGAAGGCAAGTTGTTCAAATCCAGCTACCCGAAATCTTTCAGAGATTTGGTAGGGACCAACGGTGTGATCACAGCACAAGGGGAACAACAAAGGAAACTCCATGCAATTGCCTCCAATATGATGCGCCTACATGACCCCAAATTTCAGTTCCTGCGAGATATTCAAATCATAATCATTCAAACCTTGGGAAGTTTCCATAACGGCCAACTCATATGTCTCCAAGACGTTTGCAGGAAGATAGCTATTAATCTAATGGTAAGTCAGGTACTAGGAGTGTCAAGTGAATCAGAAGTGGAAGAAATTAGTGGGTTATTTTACGACTTCGTGGATGGCTGTTTGTCTATTCCTATCAACTTTCCTGGCTTTGCCTATTACAGGGCCATGAAGGCGAGGGAGCAAATCATAGGCAAAATTAAGAAGAAAATAGCGTGGCATAAGGCGAGGATGGCAGAGGGAAGGAGCGGGGAGGGAATGTTGGGAGGAGTATTGGAGGACCAGAAGTTGTCGGATGAGGCAGTTGCTGATTTCATTATCAATCTTCTTTTTGCTGGGAATGAAACCACATCTAAAACAATGCTTTTTGCAGTCTATTTCCTAACCCACTCACCCACAGCAATGAAGCAACTTGTGGATGAGCAAGAGGGTTTAACAAACAAGAATGGAGATCATACAATCACATGGCAGGATTACAAATCGATGCCCTTTACTCAATGTGTTATTGACGAGACACTTCGACTGGGAGGAATTGCAATATGGTTGATGAGGGAGGCAAAAGAAGAGATCAAGTATTCAGATTACGTTATTCCGAAAGGATGTTTCGTTGTTCCATTTCTTTCTGCTGTACATTTGGACGAGACTATATATGGAGAAGCTTCAACTTTCAACCCTTGGAGATGGATGGAGCTTAAGAATGAGGAAATGAGAAACTGGAGGAGTAGCCCATTCTTTGCTCCATTCGGGGGAGGTGCTAGATTATGTCCGGGAGCAGAATTGGCGCGCCTCCAAATTGCTCTATTTCTCCACCACTTTGTTACCAAGTATAGGTCTTCCACTTCCAGCTTCGTTTTCATTGTGAATTGCTCTCTGCGTTTTTCGCCATTCCTTCCCTTTCACTCTACCCACCTAACTCCCACTCCCACTCCCGCTAGGGTTCCTTCGGGTTTTTCCCTCTCCAAATGTTCCTTTACTTGGATTCCATGCTACGCGTAG

Coding sequence (CDS)

ATGAGAATGTGGGAGGCCTCACCCTGGTCCCTGCTGTGCGTTCTTACAGGGCTGTACGCGCTTGTGTTGGTTGCAAGAACACGAAGAAAACGAAAGGCGAGAGCATACAGGTTTCCTCCAGGGAGGAGAAGCTGGCCTCTAATAGGCGATAGCTTGAAGTGGTACAGTGCGGTGGCAAGCTCTCATCCGCCCAAGTATGTTGAAGAACAGGCCAAAAGGTACGGGAAGATCTTCTCGTGCCGTGTGTTCGGGAAATGGGCAGTGGTGTCGGTGGATCCCGTGTTTAACCGATACGTCATGCAAAACGAAGGCAAGTTGTTCAAATCCAGCTACCCGAAATCTTTCAGAGATTTGGTAGGGACCAACGGTGTGATCACAGCACAAGGGGAACAACAAAGGAAACTCCATGCAATTGCCTCCAATATGATGCGCCTACATGACCCCAAATTTCAGTTCCTGCGAGATATTCAAATCATAATCATTCAAACCTTGGGAAGTTTCCATAACGGCCAACTCATATGTCTCCAAGACGTTTGCAGGAAGATAGCTATTAATCTAATGGTAAGTCAGGTACTAGGAGTGTCAAGTGAATCAGAAGTGGAAGAAATTAGTGGGTTATTTTACGACTTCGTGGATGGCTGTTTGTCTATTCCTATCAACTTTCCTGGCTTTGCCTATTACAGGGCCATGAAGGCGAGGGAGCAAATCATAGGCAAAATTAAGAAGAAAATAGCGTGGCATAAGGCGAGGATGGCAGAGGGAAGGAGCGGGGAGGGAATGTTGGGAGGAGTATTGGAGGACCAGAAGTTGTCGGATGAGGCAGTTGCTGATTTCATTATCAATCTTCTTTTTGCTGGGAATGAAACCACATCTAAAACAATGCTTTTTGCAGTCTATTTCCTAACCCACTCACCCACAGCAATGAAGCAACTTGTGGATGAGCAAGAGGGTTTAACAAACAAGAATGGAGATCATACAATCACATGGCAGGATTACAAATCGATGCCCTTTACTCAATGTGTTATTGACGAGACACTTCGACTGGGAGGAATTGCAATATGGTTGATGAGGGAGGCAAAAGAAGAGATCAAGTATTCAGATTACGTTATTCCGAAAGGATGTTTCGTTGTTCCATTTCTTTCTGCTGTACATTTGGACGAGACTATATATGGAGAAGCTTCAACTTTCAACCCTTGGAGATGGATGGAGCTTAAGAATGAGGAAATGAGAAACTGGAGGAGTAGCCCATTCTTTGCTCCATTCGGGGGAGGTGCTAGATTATGTCCGGGAGCAGAATTGGCGCGCCTCCAAATTGCTCTATTTCTCCACCACTTTGTTACCAAGTATAGGTCTTCCACTTCCAGCTTCGTTTTCATTGTGAATTGCTCTCTGCGTTTTTCGCCATTCCTTCCCTTTCACTCTACCCACCTAACTCCCACTCCCACTCCCGCTAGGGTTCCTTCGGGTTTTTCCCTCTCCAAATGTTCCTTTACTTGGATTCCATGCTACGCGTAG

Protein sequence

MRMWEASPWSLLCVLTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYRSSTSSFVFIVNCSLRFSPFLPFHSTHLTPTPTPARVPSGFSLSKCSFTWIPCYA
BLAST of CmaCh08G013100 vs. Swiss-Prot
Match: C72B1_PINTA (Abietadienol/abietadienal oxidase OS=Pinus taeda GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.5e-102
Identity = 196/452 (43.36%), Postives = 282/452 (62.39%), Query Frame = 1

Query: 11  LLCVLTGLYALVLV------ARTRRKR----KARAYRFPPGRRSWPLIGDSLKWYSAVAS 70
           LL V T   AL+ +      A+  +KR    K +    PPG   WPLIG++  +Y ++ S
Sbjct: 8   LLVVFTAAVALLHLIYRWWNAQRGQKRTSNEKNQELHLPPGSTGWPLIGETYSYYRSMTS 67

Query: 71  SHPPKYVEEQAKRYGK-IFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLV 130
           + P ++++++ KRY   +F   +FG  AV+S DP FN+YV+QNEG+ F++ YPK+ + L+
Sbjct: 68  NRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQFNKYVLQNEGRFFQAHYPKALKALI 127

Query: 131 GTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVC 190
           G  G+++  G+ QRKLH IA N++R    KF F+ +IQ ++  TL  + + + I LQ+ C
Sbjct: 128 GDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEIQNLVHSTLDRWVDKKEIALQNEC 187

Query: 191 RKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGK 250
            ++ +NLM  Q+L +S   E  EI  LF D+ +  ++IPI  PG  Y + +KARE +I K
Sbjct: 188 HQMVLNLMAKQLLDLSPSKETNEICELFVDYTNAVIAIPIKIPGSTYAKGLKARELLIRK 247

Query: 251 IKKKIAWHKARMAEGRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVY 310
           I   I   + R       + +L  +LE+  +SDE + DFI+ LLFAG+ET+S+ M FA+ 
Sbjct: 248 ISNMI--KERRDHPHIVHKDLLTKLLEEDSISDEIICDFILFLLFAGHETSSRAMTFAIK 307

Query: 311 FLTHSPTAMKQLVDEQEGLTNKNGDH-TITWQDYKSMPFTQCVIDETLRLGGIAIWLMRE 370
           FLT  P A+ Q+ +E + +    G H  + W DYKSM FTQCVI+ETLRLG     + RE
Sbjct: 308 FLTTCPKALTQMKEEHDAILKAKGGHKKLEWDDYKSMKFTQCVINETLRLGNFGPGVFRE 367

Query: 371 AKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFF 430
            KE+ K  D +IPKG  V  FL+A HLDE  + EA TFNPWRW EL  +   N      F
Sbjct: 368 TKEDTKVKDCLIPKGWVVFAFLTATHLDEKFHNEALTFNPWRW-ELDQDVSNN----HLF 427

Query: 431 APFGGGARLCPGAELARLQIALFLHHFVTKYR 451
           +PFGGGARLCPG+ LARL++ALFLH F+T++R
Sbjct: 428 SPFGGGARLCPGSHLARLELALFLHIFITRFR 452

BLAST of CmaCh08G013100 vs. Swiss-Prot
Match: C72B2_PINTA (Cytochrome P450 720B2 OS=Pinus taeda GN=CYP720B2 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.6e-96
Identity = 189/452 (41.81%), Postives = 282/452 (62.39%), Query Frame = 1

Query: 11  LLCVLTG-----LYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPK 70
           LLC++ G     LY LV  A       +RAY+ PPG   WPLIG+++ ++  + S+  P+
Sbjct: 18  LLCLVLGFLLLQLYKLVWGA------SSRAYKLPPGSTGWPLIGETISFFRGINSTAQPR 77

Query: 71  -YVEEQAKRYGKIFSCRVFGKWA-VVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNG 130
            +++E+ +RYG+IF   +FG+   VVSVDP FN++V+Q+EG+ F+++YPK  R+L+G  G
Sbjct: 78  QFIQEREQRYGEIFRSNLFGRSRIVVSVDPEFNKHVLQHEGRQFQANYPKPLRNLIGKYG 137

Query: 131 VITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIA 190
           +++  G+ QRKLH  A N++R       F+ DIQ ++  TL  +   + I LQ+ C ++ 
Sbjct: 138 LLSVHGDLQRKLHGAAVNLLRFERLSVDFMEDIQNLLHITLAKWEAKRDIHLQEECHQLV 197

Query: 191 INLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKK 250
           +NLM  Q+L +S   + EEI   F  F +  L++PI  PG  Y R  KARE +I KI + 
Sbjct: 198 LNLMAKQLLDLSPSKDTEEICEAFGHFSEALLAVPIKIPGTKYARGFKAREFLIKKIYES 257

Query: 251 IAWHKARMAEGRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTH 310
           I     R         +L  +L++   S+E +ADFI+ LLFAG+ET+S++M FA+ FLT 
Sbjct: 258 I--EDRRQHPEAVHNDLLTKLLKEDSFSEEIIADFILFLLFAGHETSSRSMSFAIKFLTD 317

Query: 311 SPTAMKQLVDEQEGLTNKNG---DHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAK 370
            P A+++L  E + L  + G   +  + W DY+S+ FTQCVI ETLR+G     + RE K
Sbjct: 318 CPRALEELKAEHDALLKRKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPGVFRETK 377

Query: 371 EEIK-YSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWM-ELKNEEMRNWRSSPFF 430
           E+IK    +VIP+G  V  FL+  HLDE  +  A  F+PWRW   L+++E+     +P F
Sbjct: 378 EDIKTKGGFVIPRGWTVYVFLTGTHLDEKYHSSALKFDPWRWQPHLQDQEL---LKNPSF 437

Query: 431 APFGGGARLCPGAELARLQIALFLHHFVTKYR 451
            PFGGGARLCPG  LA++++ALFLH+FVTK+R
Sbjct: 438 MPFGGGARLCPGMHLAKMELALFLHNFVTKFR 458

BLAST of CmaCh08G013100 vs. Swiss-Prot
Match: C90B1_ARATH (Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 302.8 bits (774), Expect = 7.2e-81
Identity = 155/464 (33.41%), Postives = 259/464 (55.82%), Query Frame = 1

Query: 21  LVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSC 80
           L L+   RR RK R +  PPG+  WP +G+++ +     ++    ++++   +YGKI+  
Sbjct: 23  LFLILLKRRNRKTR-FNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRS 82

Query: 81  RVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIAS 140
            +FG+  +VS D   NR+++QNEG+LF+ SYP+S   ++G   ++   G+  R + +I+ 
Sbjct: 83  NLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISL 142

Query: 141 NMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVS-SESE 200
           N +     +   L+D++   +  L S+    +   QD  +K   NLM   ++ +   E E
Sbjct: 143 NFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEE 202

Query: 201 VEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEG----- 260
            E++   +  F+ G +S P+N PG AY++A+++R  I+  I++K+   K  + E      
Sbjct: 203 TEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEE 262

Query: 261 -------------------RSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTML 320
                              R+ + +LG VL+   LS E + D I++LLFAG+ET+S  + 
Sbjct: 263 EVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIA 322

Query: 321 FAVYFLTHSPTAMKQLVDEQEGLTNKN---GDHTITWQDYKSMPFTQCVIDETLRLGGIA 380
            A++FL   P A+++L +E   +       G+  + W DYK M FTQCVI+ETLRLG + 
Sbjct: 323 LAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVV 382

Query: 381 IWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKN------ 440
            +L R+A ++++Y  Y IP G  V+P +SAVHLD + Y + + FNPWRW +  N      
Sbjct: 383 RFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSG 442

Query: 441 -EEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKY 450
                 W ++  + PFGGG RLC G+ELA+L++A+F+HH V K+
Sbjct: 443 SGSFSTWGNN--YMPFGGGPRLCAGSELAKLEMAVFIHHLVLKF 483

BLAST of CmaCh08G013100 vs. Swiss-Prot
Match: C90D1_ARATH (3-epi-6-deoxocathasterone 23-monooxygenase OS=Arabidopsis thaliana GN=CYP90D1 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 2.9e-74
Identity = 139/424 (32.78%), Postives = 248/424 (58.49%), Query Frame = 1

Query: 37  RFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFN 96
           +FP G   WP+IG+++++ S+  S  P  +++++   YG++F   +FG   +VS D   N
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 97  RYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDI 156
           R V+Q++   F   YPK+ R+L+G + ++   G   R+ H +  + ++    K Q +RD+
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 157 QIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLS 216
              + +++  +   Q + LQDV + +A  ++   ++ V    ++EE+   F +F+ G +S
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 217 IPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLEDQKLSDEA-- 276
           +PINFPG   +R+++A++ ++ ++ ++I   K R  + +  + ++   + D  L D +  
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQV-ERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH 289

Query: 277 -----VADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQ---EGLTNKNGDHT 336
                +A+ +I+++  G+++    +  AV FL+ SP A+  L +E    + L    G+  
Sbjct: 290 LTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGE-P 349

Query: 337 ITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLD 396
           + W DY S+PFTQ VI ETLR+G + I +MR+A ++++   YVIPKG   + +L +VHLD
Sbjct: 350 LYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLD 409

Query: 397 ETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFV 451
           +  Y     FNPWRW E      R+  +S  F+PFGGG RLCPG +LARL+ ++FLHH V
Sbjct: 410 KLYYESPYKFNPWRWQE------RDMNTSS-FSPFGGGQRLCPGLDLARLETSVFLHHLV 464

BLAST of CmaCh08G013100 vs. Swiss-Prot
Match: C90D2_ORYSJ (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.0e-74
Identity = 149/459 (32.46%), Postives = 256/459 (55.77%), Query Frame = 1

Query: 4   WEASPWSLLCVLTGLYALVLVARTRRKRK-------ARAYRFPPGRRSWPLIGDSLKWYS 63
           W A  +++  V+  +     + R RR+         A A R PPG   WP++G++L++ S
Sbjct: 8   WAAPAFAVAAVVIWVVLCSELLRRRRRGAGSGKGDAAAAARLPPGSFGWPVVGETLEFVS 67

Query: 64  AVASSHPPKYVEEQAKRYGK-IFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSF 123
              S  P  +V+++ K +G  +F   +FG   VV+ D   +R+V+Q++ + F   YP+S 
Sbjct: 68  CAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVSRFVLQSDARAFVPWYPRSL 127

Query: 124 RDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICL 183
            +L+G + ++   G  QR++H +     +    K Q   D++  +   L SF +  L+ +
Sbjct: 128 TELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADMRRRLSPALSSFPDSSLLHV 187

Query: 184 QDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQ 243
           Q + + +   ++V  ++G+ +  E++++   F +F+ G +S+PI  PG   YR+++A+++
Sbjct: 188 QHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLMSLPIKLPGTRLYRSLQAKKK 247

Query: 244 IIGKIKKKIAWHKARMAEGRSGEGMLGGVLED--QKLSDEAVADFIINLLFAGNETTSKT 303
           +   I++ I   +AR A        +  ++ D   +L+DE ++D +I+L+    ++    
Sbjct: 248 MARLIQRIIREKRARRAAASPPRDAIDVLIGDGSDELTDELISDNMIDLMIPAEDSVPVL 307

Query: 304 MLFAVYFLTHSPTAMKQLVDEQEGLTNKNGD--HTITWQDYKSMPFTQCVIDETLRLGGI 363
           +  AV FL+  P A+ QL +E   L  +  D   T+ W DY S+ FTQ VI ETLRLG I
Sbjct: 308 ITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWTDYMSLSFTQHVITETLRLGNI 367

Query: 364 AIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRN 423
              +MR+A  +++   ++IPKG  V  +  +VHLD+T+Y E   FNPWRW   K ++M N
Sbjct: 368 IGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLYDEPYKFNPWRW---KEKDMSN 427

Query: 424 WRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
                 F PFGGG RLCPG +LARL+ ++FLHH VT +R
Sbjct: 428 GS----FTPFGGGQRLCPGLDLARLEASIFLHHLVTSFR 459

BLAST of CmaCh08G013100 vs. TrEMBL
Match: A0A0A0K7D5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G001760 PE=3 SV=1)

HSP 1 Score: 672.5 bits (1734), Expect = 3.9e-190
Identity = 338/462 (73.16%), Postives = 378/462 (81.82%), Query Frame = 1

Query: 3   MWEAS--PWSLLCVLTGLYALVLVARTRRKR----------KARAYRFPPGRRSWPLIGD 62
           MWE S   WS   +LT L+   L    ++K+              Y+FP GRRSWP+IGD
Sbjct: 16  MWEPSLVAWSFF-ILTLLFTTFLPNILKKKKYNDQNDQNDQSRTRYKFPSGRRSWPVIGD 75

Query: 63  SLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSS 122
           S  WYSA+ASSHP KYVEEQAKRYGKIFSCRVFGK  VVSVDP FNRYVMQNEG LF+SS
Sbjct: 76  SFNWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPDFNRYVMQNEGILFQSS 135

Query: 123 YPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNG 182
           YPKSFRDLVG NGVITAQGEQQRKLH IASN MR++   F F+++IQ III +L +FH+ 
Sbjct: 136 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFIKEIQTIIIHSLTTFHDR 195

Query: 183 -QLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRA 242
            Q+I LQD CRKIAINLMVSQ+LGVSS+SEV EISGLFYDFVDGCLS PIN P FAY+ A
Sbjct: 196 HQIISLQDACRKIAINLMVSQLLGVSSDSEVNEISGLFYDFVDGCLSFPINLPPFAYHSA 255

Query: 243 MKAREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLEDQK-LSDEAVADFIINLLFAGNE 302
           MKAR+QII KIKKKI   K+      SG G+LG +LE+QK L DEAV DFIINLLFAGNE
Sbjct: 256 MKARKQIIRKIKKKIEMQKSMEKSSSSGHGVLGRLLEEQKHLCDEAVEDFIINLLFAGNE 315

Query: 303 TTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRL 362
           TTSKTMLFAVY+LTHSP A +QL++EQ+GL NK GD+T+TW+DYKSM FTQCVIDETLRL
Sbjct: 316 TTSKTMLFAVYYLTHSPKAFEQLMEEQDGLRNKCGDNTVTWEDYKSMSFTQCVIDETLRL 375

Query: 363 GGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEE 422
           GGIAIWLMRE KEEIKYSDYVIPKG FVVPFLSAVHLDE IY EA TFNPWRWM+L+N+E
Sbjct: 376 GGIAIWLMRETKEEIKYSDYVIPKGTFVVPFLSAVHLDENIYDEALTFNPWRWMQLQNQE 435

Query: 423 MRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
            RNW+SSP+FAPFGGG RLCPGAELARLQIALFLHHF+T YR
Sbjct: 436 KRNWKSSPYFAPFGGGGRLCPGAELARLQIALFLHHFLTNYR 476

BLAST of CmaCh08G013100 vs. TrEMBL
Match: V7C555_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G136300g PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 2.4e-184
Identity = 314/437 (71.85%), Postives = 366/437 (83.75%), Query Frame = 1

Query: 14  VLTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKR 73
           ++T ++A V+  + R++ K++  R PPGRR WPLIGDS+ WY+AVASSHPP++VEE  KR
Sbjct: 20  LVTAIFAKVIQFKLRKEDKSKC-RLPPGRRGWPLIGDSINWYNAVASSHPPQFVEEMVKR 79

Query: 74  YGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQR 133
           Y KIFSC +FGKWAVVS DP FNR+VMQNEG+LF SSYPKSFRDLVG NGVIT QGEQQR
Sbjct: 80  YDKIFSCSLFGKWAVVSADPSFNRFVMQNEGRLFISSYPKSFRDLVGKNGVITVQGEQQR 139

Query: 134 KLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLG 193
           KLH IASNMMRL   KF FL DIQ +++QTLG+F+N Q+I LQDVCRK+AI+LMV+Q+LG
Sbjct: 140 KLHGIASNMMRLEKLKFHFLNDIQKVMLQTLGNFNNNQVILLQDVCRKVAIHLMVNQLLG 199

Query: 194 VSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAE 253
           VSSES+V E++ LF DFVDGCLSIPIN PG+AY+ AMKARE II KI K I  H+   A 
Sbjct: 200 VSSESQVSELAQLFSDFVDGCLSIPINIPGYAYHTAMKARENIISKINKIIEEHRQNGAP 259

Query: 254 GRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVD 313
              G G+LG +LE++ L D+AVADFIINLLFAGNETT+KTMLFAVYFLT  P AMKQL+D
Sbjct: 260 -MEGNGVLGRLLEEESLPDDAVADFIINLLFAGNETTTKTMLFAVYFLTQCPRAMKQLLD 319

Query: 314 EQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKG 373
           E   L + +GD  +TWQDYK+MPFTQCVIDETLRLGGIAIWLMREAKE+++Y D+VIPKG
Sbjct: 320 EHGSLRSNSGDELLTWQDYKAMPFTQCVIDETLRLGGIAIWLMREAKEDVQYQDFVIPKG 379

Query: 374 CFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAEL 433
           CFVVPFLSAVHLDE +Y  A  FNPWRWME +NEE RNWRSSPF+APFGGGAR CPGAEL
Sbjct: 380 CFVVPFLSAVHLDEKVYSGALNFNPWRWMEHENEEKRNWRSSPFYAPFGGGARFCPGAEL 439

Query: 434 ARLQIALFLHHFVTKYR 451
           ARLQIALFLH+FVT YR
Sbjct: 440 ARLQIALFLHYFVTTYR 454

BLAST of CmaCh08G013100 vs. TrEMBL
Match: A0A0L9VHS8_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan10g051700 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.7e-183
Identity = 312/437 (71.40%), Postives = 366/437 (83.75%), Query Frame = 1

Query: 14  VLTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKR 73
           ++T ++A +++ +  ++ K R  R PPGRR WPLIGDS+ WY+AVASSHPP++VEE  KR
Sbjct: 20  LVTAIFAKIILFKLGKEDK-RKCRLPPGRRGWPLIGDSINWYNAVASSHPPQFVEEMVKR 79

Query: 74  YGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQR 133
           YGKIFSC +FGKWAVVS DP FNR+VMQNEG+LF SSYPKSFRDLVG NGVIT QGEQQR
Sbjct: 80  YGKIFSCSLFGKWAVVSADPSFNRFVMQNEGRLFISSYPKSFRDLVGKNGVITVQGEQQR 139

Query: 134 KLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLG 193
           KLH IASNMMRL   KF FL DIQ +++QTLG+F+N Q+I LQDVCRK+AI+LMV+Q+LG
Sbjct: 140 KLHGIASNMMRLEKLKFHFLNDIQKVMVQTLGNFNNNQVILLQDVCRKVAIHLMVNQLLG 199

Query: 194 VSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAE 253
           VSSES+V E++ LF DFVDGCLSIPIN PG+AY+ AMKARE II KI K I  H+ + A 
Sbjct: 200 VSSESQVNEMAQLFSDFVDGCLSIPINIPGYAYHTAMKARENIISKINKIIEEHRQKGAP 259

Query: 254 GRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVD 313
              G G+LG +LE++ L D+AVADFIINLLFAGNETT+KTMLFAVYFLT  P AMKQL++
Sbjct: 260 -MEGSGVLGRLLEEESLPDDAVADFIINLLFAGNETTTKTMLFAVYFLTQCPRAMKQLLE 319

Query: 314 EQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKG 373
           E   L + +GD  +TWQDYK+M FTQCVIDETLRLGGIAIWLMREAKE+++Y D++IPKG
Sbjct: 320 EHSSLRSDSGDELLTWQDYKAMSFTQCVIDETLRLGGIAIWLMREAKEDVQYQDFIIPKG 379

Query: 374 CFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAEL 433
           CFVVPFLSAVHLDE IY  A  FNPWRWME +NEE RNWRSSPF+APFGGGAR CPGAEL
Sbjct: 380 CFVVPFLSAVHLDEKIYSGALDFNPWRWMEHENEEKRNWRSSPFYAPFGGGARFCPGAEL 439

Query: 434 ARLQIALFLHHFVTKYR 451
           ARLQIALFLH+FVT YR
Sbjct: 440 ARLQIALFLHYFVTTYR 454

BLAST of CmaCh08G013100 vs. TrEMBL
Match: A0A0S3TBJ6_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G209500 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.7e-183
Identity = 312/437 (71.40%), Postives = 366/437 (83.75%), Query Frame = 1

Query: 14  VLTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKR 73
           ++T ++A +++ +  ++ K R  R PPGRR WPLIGDS+ WY+AVASSHPP++VEE  KR
Sbjct: 20  LVTAIFAKIILFKLGKEDK-RKCRLPPGRRGWPLIGDSINWYNAVASSHPPQFVEEMVKR 79

Query: 74  YGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQR 133
           YGKIFSC +FGKWAVVS DP FNR+VMQNEG+LF SSYPKSFRDLVG NGVIT QGEQQR
Sbjct: 80  YGKIFSCSLFGKWAVVSADPSFNRFVMQNEGRLFISSYPKSFRDLVGKNGVITVQGEQQR 139

Query: 134 KLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLG 193
           KLH IASNMMRL   KF FL DIQ +++QTLG+F+N Q+I LQDVCRK+AI+LMV+Q+LG
Sbjct: 140 KLHGIASNMMRLEKLKFHFLNDIQKVMVQTLGNFNNNQVILLQDVCRKVAIHLMVNQLLG 199

Query: 194 VSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAE 253
           VSSES+V E++ LF DFVDGCLSIPIN PG+AY+ AMKARE II KI K I  H+ + A 
Sbjct: 200 VSSESQVNEMAQLFSDFVDGCLSIPINIPGYAYHTAMKARENIISKINKIIEEHRQKGAP 259

Query: 254 GRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVD 313
              G G+LG +LE++ L D+AVADFIINLLFAGNETT+KTMLFAVYFLT  P AMKQL++
Sbjct: 260 -MEGSGVLGRLLEEESLPDDAVADFIINLLFAGNETTTKTMLFAVYFLTQCPRAMKQLLE 319

Query: 314 EQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKG 373
           E   L + +GD  +TWQDYK+M FTQCVIDETLRLGGIAIWLMREAKE+++Y D++IPKG
Sbjct: 320 EHSSLRSDSGDELLTWQDYKAMSFTQCVIDETLRLGGIAIWLMREAKEDVQYQDFIIPKG 379

Query: 374 CFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAEL 433
           CFVVPFLSAVHLDE IY  A  FNPWRWME +NEE RNWRSSPF+APFGGGAR CPGAEL
Sbjct: 380 CFVVPFLSAVHLDEKIYSGALDFNPWRWMEHENEEKRNWRSSPFYAPFGGGARFCPGAEL 439

Query: 434 ARLQIALFLHHFVTKYR 451
           ARLQIALFLH+FVT YR
Sbjct: 440 ARLQIALFLHYFVTTYR 454

BLAST of CmaCh08G013100 vs. TrEMBL
Match: A0A072UB54_MEDTR (Cytochrome P450 family protein OS=Medicago truncatula GN=MTR_6g069000 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.0e-182
Identity = 309/440 (70.23%), Postives = 364/440 (82.73%), Query Frame = 1

Query: 11  LLCVLTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQ 70
           ++ + T ++A V+  + R++ K+   R PPGRR WPLIGDS+ WY+AVASSHPP++VEE 
Sbjct: 24  IVIMFTAIFAKVIQIKKRKEEKSIG-RLPPGRRGWPLIGDSINWYNAVASSHPPQFVEEM 83

Query: 71  AKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGE 130
            +RYGKIFSC +FGKWAVVS DP FNR+VMQNEGKLF SSYPKSFRDLVG NGVIT QGE
Sbjct: 84  VQRYGKIFSCSLFGKWAVVSSDPSFNRFVMQNEGKLFMSSYPKSFRDLVGKNGVITVQGE 143

Query: 131 QQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQ 190
           QQRKLH IASNMMRL   KF F+ DIQ ++IQTL +F N Q+I LQDVCRK+AINLMV+Q
Sbjct: 144 QQRKLHGIASNMMRLDKLKFHFMNDIQNVMIQTLSNFKNQQVILLQDVCRKVAINLMVNQ 203

Query: 191 VLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKAR 250
           +LGVSSES+V E++ LF DFVDGCLS+PIN PG +Y+ AMKARE+II KI K I   +  
Sbjct: 204 LLGVSSESQVNEMAQLFSDFVDGCLSVPINIPGSSYHTAMKAREKIISKINKIIEVQRQN 263

Query: 251 MAEGRSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQ 310
                   G+LG ++E+  L D+AVADFIINLLFAGNETT+KTMLFAVYFLT  P AM+Q
Sbjct: 264 GPPKEGNNGVLGRLIEEDSLPDDAVADFIINLLFAGNETTTKTMLFAVYFLTQCPNAMEQ 323

Query: 311 LVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVI 370
           L+DEQ+ L  K+ + ++TWQDYK+MPFTQCVIDETLRLGGIAIWL+REAKE+I+Y D+VI
Sbjct: 324 LLDEQDSLRTKSAEESLTWQDYKAMPFTQCVIDETLRLGGIAIWLLREAKEDIQYQDFVI 383

Query: 371 PKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPG 430
           PKGCFVVPFLSAVHLDE +Y EA  FNPWRWME +NEE RNWRSSPF+APFGGGAR CPG
Sbjct: 384 PKGCFVVPFLSAVHLDEKVYNEAKNFNPWRWMEPENEEKRNWRSSPFYAPFGGGARFCPG 443

Query: 431 AELARLQIALFLHHFVTKYR 451
           AELARLQIALFLH+FVT YR
Sbjct: 444 AELARLQIALFLHYFVTNYR 462

BLAST of CmaCh08G013100 vs. TAIR10
Match: AT1G73340.1 (AT1G73340.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 570.1 bits (1468), Expect = 1.4e-162
Identity = 279/464 (60.13%), Postives = 348/464 (75.00%), Query Frame = 1

Query: 15  LTGLYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRY 74
           L  +   +L    RRKR+A  +R PPG R WPLIGD+  W +AVA SHP  +VE+Q K+Y
Sbjct: 21  LAFIIIFLLAGIARRKRRA-PHRLPPGSRGWPLIGDTFAWLNAVAGSHPSSFVEKQIKKY 80

Query: 75  GKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRK 134
           G+IFSC +FGKWAVVS DP FNR++MQNEGKLF+SSYPKSFRDLVG +GVIT  G+QQR+
Sbjct: 81  GRIFSCSLFGKWAVVSADPDFNRFIMQNEGKLFQSSYPKSFRDLVGKDGVITVHGDQQRR 140

Query: 135 LHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGV 194
           LH+IAS+MMR    K  FL  I ++++QTL +F +G+++ LQD+CRK+AI+LMV+Q+LGV
Sbjct: 141 LHSIASSMMRHDQLKTHFLEVIPVVMLQTLSNFKDGEVVLLQDICRKVAIHLMVNQLLGV 200

Query: 195 SSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQ------------------- 254
           SSESEV+E+S LF DFVDGCLS+PI+ PGF Y +AMK   +                   
Sbjct: 201 SSESEVDEMSQLFSDFVDGCLSVPIDLPGFTYNKAMKVSFKHLSQLLICGFGACLLRFCL 260

Query: 255 --------IIGKIKKKIAWH-KARMAEGRSGEGMLGGVLEDQKLSDEAVADFIINLLFAG 314
                   II KI K I    + + A   +G G+LG +LE++ L +E++ADFIINLLFAG
Sbjct: 261 FLIQARKEIIRKINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAG 320

Query: 315 NETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDETL 374
           NETTSKTMLFAVYFLTH P AM QL++E + L        +TWQDYK+M FTQCVIDETL
Sbjct: 321 NETTSKTMLFAVYFLTHCPKAMTQLLEEHDRLAG----GMLTWQDYKTMDFTQCVIDETL 380

Query: 375 RLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKN 434
           RLGGIAIWLMREAKE++ Y DYVIPKGCFVVPFLSAVHLDE+ Y E+ +FNPWRW++ + 
Sbjct: 381 RLGGIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHLDESYYKESLSFNPWRWLDPET 440

Query: 435 EEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
           ++ RNWR+SPF+ PFGGG R CPGAELARLQIALFLH+F+T Y+
Sbjct: 441 QQKRNWRTSPFYCPFGGGTRFCPGAELARLQIALFLHYFITTYK 479

BLAST of CmaCh08G013100 vs. TAIR10
Match: AT3G50660.1 (AT3G50660.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 302.8 bits (774), Expect = 4.1e-82
Identity = 155/464 (33.41%), Postives = 259/464 (55.82%), Query Frame = 1

Query: 21  LVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSC 80
           L L+   RR RK R +  PPG+  WP +G+++ +     ++    ++++   +YGKI+  
Sbjct: 23  LFLILLKRRNRKTR-FNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRS 82

Query: 81  RVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIAS 140
            +FG+  +VS D   NR+++QNEG+LF+ SYP+S   ++G   ++   G+  R + +I+ 
Sbjct: 83  NLFGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISL 142

Query: 141 NMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVS-SESE 200
           N +     +   L+D++   +  L S+    +   QD  +K   NLM   ++ +   E E
Sbjct: 143 NFLSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEE 202

Query: 201 VEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEG----- 260
            E++   +  F+ G +S P+N PG AY++A+++R  I+  I++K+   K  + E      
Sbjct: 203 TEQLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEE 262

Query: 261 -------------------RSGEGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTML 320
                              R+ + +LG VL+   LS E + D I++LLFAG+ET+S  + 
Sbjct: 263 EVKTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIA 322

Query: 321 FAVYFLTHSPTAMKQLVDEQEGLTNKN---GDHTITWQDYKSMPFTQCVIDETLRLGGIA 380
            A++FL   P A+++L +E   +       G+  + W DYK M FTQCVI+ETLRLG + 
Sbjct: 323 LAIFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVV 382

Query: 381 IWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKN------ 440
            +L R+A ++++Y  Y IP G  V+P +SAVHLD + Y + + FNPWRW +  N      
Sbjct: 383 RFLHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSG 442

Query: 441 -EEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKY 450
                 W ++  + PFGGG RLC G+ELA+L++A+F+HH V K+
Sbjct: 443 SGSFSTWGNN--YMPFGGGPRLCAGSELAKLEMAVFIHHLVLKF 483

BLAST of CmaCh08G013100 vs. TAIR10
Match: AT3G13730.1 (AT3G13730.1 cytochrome P450, family 90, subfamily D, polypeptide 1)

HSP 1 Score: 280.8 bits (717), Expect = 1.7e-75
Identity = 139/424 (32.78%), Postives = 248/424 (58.49%), Query Frame = 1

Query: 37  RFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFN 96
           +FP G   WP+IG+++++ S+  S  P  +++++   YG++F   +FG   +VS D   N
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 97  RYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDI 156
           R V+Q++   F   YPK+ R+L+G + ++   G   R+ H +  + ++    K Q +RD+
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 157 QIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLS 216
              + +++  +   Q + LQDV + +A  ++   ++ V    ++EE+   F +F+ G +S
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 217 IPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLEDQKLSDEA-- 276
           +PINFPG   +R+++A++ ++ ++ ++I   K R  + +  + ++   + D  L D +  
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQV-ERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH 289

Query: 277 -----VADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQ---EGLTNKNGDHT 336
                +A+ +I+++  G+++    +  AV FL+ SP A+  L +E    + L    G+  
Sbjct: 290 LTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGE-P 349

Query: 337 ITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLD 396
           + W DY S+PFTQ VI ETLR+G + I +MR+A ++++   YVIPKG   + +L +VHLD
Sbjct: 350 LYWNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLD 409

Query: 397 ETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFV 451
           +  Y     FNPWRW E      R+  +S  F+PFGGG RLCPG +LARL+ ++FLHH V
Sbjct: 410 KLYYESPYKFNPWRWQE------RDMNTSS-FSPFGGGQRLCPGLDLARLETSVFLHHLV 464

BLAST of CmaCh08G013100 vs. TAIR10
Match: AT4G36380.1 (AT4G36380.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-74
Identity = 141/419 (33.65%), Postives = 231/419 (55.13%), Query Frame = 1

Query: 39  PPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRY 98
           P G   WP+IG++L + +   SS P  +++++   YGK+F   + G   ++S D   N+ 
Sbjct: 70  PNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKV 129

Query: 99  VMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQI 158
           V+QN G  F  +YPKS  +L+G N +++  G  Q++LH +    +R    K +  RDI+ 
Sbjct: 130 VLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEA 189

Query: 159 IIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIP 218
            ++ TL S+    L+ +QD  +K+   ++V  ++  S   ++  +   F +F+ G + IP
Sbjct: 190 SVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIP 249

Query: 219 INFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEGRSGEGML------GGVLEDQKLSD 278
           I FPG   Y+++KA+E++I  +KK +   +  M        ++      GG  E Q    
Sbjct: 250 IKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQPS 309

Query: 279 EAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGD--HTITWQ 338
           + V+  I+ ++  G ET    M  AV FL+ +P A+ +LV+E   +  +  +      W 
Sbjct: 310 DFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWT 369

Query: 339 DYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIY 398
           DY S+ FTQ VI+ETLR+  I   + R+A ++++   Y+IPKG  V+    +VH+DE IY
Sbjct: 370 DYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIY 429

Query: 399 GEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKY 450
                F+PWRW  +         SS  F PFGGG RLCPG EL++L+I++FLHH VT+Y
Sbjct: 430 DNPYQFDPWRWDRINGSA----NSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRY 484

BLAST of CmaCh08G013100 vs. TAIR10
Match: AT5G05690.1 (AT5G05690.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 275.4 bits (703), Expect = 7.0e-74
Identity = 157/445 (35.28%), Postives = 242/445 (54.38%), Query Frame = 1

Query: 10  SLLCVLTGLYA--LVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYV 69
           + L +L+ + A  L+L+ RTR +R       PPG    PLIG++ +   A  + +P  ++
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMG----LPPGSLGLPLIGETFQLIGAYKTENPEPFI 64

Query: 70  EEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITA 129
           +E+  RYG +F   +FG+  + S DP  NR+V+QNEGKLF+ SYP S  +L+G + ++  
Sbjct: 65  DERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLM 124

Query: 130 QGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLM 189
           +G   +++H++  +       K   + DI  ++   L S+ +  L  L +  +KI   L 
Sbjct: 125 KGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL--LMEEAKKITFELT 184

Query: 190 VSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWH 249
           V Q++        E +   +   ++G  S+P+      Y +A++AR ++   +   +   
Sbjct: 185 VKQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKR 244

Query: 250 KARMAEGRSGE-GMLGGVLE-DQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSP 309
           +    EG   +  ML  +L  D   SDE + DF++ LL AG ETTS  M  AV FLT +P
Sbjct: 245 REEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETP 304

Query: 310 TAMKQLVDEQEGL-TNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIK 369
            A+ QL +E E +   K+  +++ W DYKSMPFTQCV++ETLR+  I   + R A  +++
Sbjct: 305 LALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVE 364

Query: 370 YSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGG 429
              Y IPKG  V     AVHLD   + +A TFNPWRW             S  F PFGGG
Sbjct: 365 IKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRW----QSNSVTTGPSNVFTPFGGG 424

Query: 430 ARLCPGAELARLQIALFLHHFVTKY 450
            RLCPG ELAR+ +++FLH  VT +
Sbjct: 425 PRLCPGYELARVALSVFLHRLVTGF 439

BLAST of CmaCh08G013100 vs. NCBI nr
Match: gi|659116813|ref|XP_008458272.1| (PREDICTED: abietadienol/abietadienal oxidase isoform X2 [Cucumis melo])

HSP 1 Score: 674.9 bits (1740), Expect = 1.1e-190
Identity = 346/466 (74.25%), Postives = 381/466 (81.76%), Query Frame = 1

Query: 1   MRMWEAS--PWSLLCVLTGLYALVLVARTRRKR--------KARAYRFPPGRRSWPLIGD 60
           M MWE+S   WS   + T L A  L    + K+            Y+FPPGRRSWPLIGD
Sbjct: 1   MTMWESSLVVWSFF-IFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPGRRSWPLIGD 60

Query: 61  SLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSS 120
           S  WYSA+ASSHP KYVEEQAKRYGKIFSCRVFGK  VVSVDP FNRYVMQNEG LF+SS
Sbjct: 61  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 120

Query: 121 YPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNG 180
           YPKSFRDLVG NGVITAQGEQQRKLH IASN MR++   F FL++IQ III +L +FH+ 
Sbjct: 121 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDR 180

Query: 181 -QLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRA 240
            Q+I LQD CRKIAIN+MVSQ+LGVSS+SEV EISGLFYDFVDGCLS PIN P FAYY A
Sbjct: 181 HQIISLQDACRKIAINVMVSQLLGVSSDSEVNEISGLFYDFVDGCLSFPINLPPFAYYSA 240

Query: 241 MKAREQIIGKIKKKIAWHKA--RMAEGRS--GEGMLGGVLEDQK-LSDEAVADFIINLLF 300
           MKAR+QII KIKKK+   K+  RM+   S  G G+L  +LE+QK L DEAV DFIINLLF
Sbjct: 241 MKARKQIIRKIKKKMKMQKSMERMSSSSSSSGHGVLARLLEEQKHLCDEAVEDFIINLLF 300

Query: 301 AGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDE 360
           AGNETTSKTMLFAVY+LTHSP A  QL+DEQ+GL N+ GD+TITWQDYKSM FTQCVIDE
Sbjct: 301 AGNETTSKTMLFAVYYLTHSPKAFNQLLDEQDGLRNRCGDNTITWQDYKSMSFTQCVIDE 360

Query: 361 TLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMEL 420
           TLRLGGIAIWLMRE KEEIKYSDYVIPKG FVVPFLSAVHLDE IY EA TFNPWRWM+L
Sbjct: 361 TLRLGGIAIWLMRETKEEIKYSDYVIPKGTFVVPFLSAVHLDENIYDEALTFNPWRWMQL 420

Query: 421 KNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
           +N+E RNW+SSP+FAPFGGGARLCPGAELARLQIALFLHHFVT YR
Sbjct: 421 QNQEKRNWKSSPYFAPFGGGARLCPGAELARLQIALFLHHFVTNYR 465

BLAST of CmaCh08G013100 vs. NCBI nr
Match: gi|659116811|ref|XP_008458271.1| (PREDICTED: abietadienol/abietadienal oxidase isoform X1 [Cucumis melo])

HSP 1 Score: 674.9 bits (1740), Expect = 1.1e-190
Identity = 346/466 (74.25%), Postives = 381/466 (81.76%), Query Frame = 1

Query: 1   MRMWEAS--PWSLLCVLTGLYALVLVARTRRKR--------KARAYRFPPGRRSWPLIGD 60
           M MWE+S   WS   + T L A  L    + K+            Y+FPPGRRSWPLIGD
Sbjct: 4   MTMWESSLVVWSFF-IFTLLSATFLSNIFKNKKYNNNQNDQSQTGYKFPPGRRSWPLIGD 63

Query: 61  SLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSS 120
           S  WYSA+ASSHP KYVEEQAKRYGKIFSCRVFGK  VVSVDP FNRYVMQNEG LF+SS
Sbjct: 64  SFDWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPEFNRYVMQNEGILFQSS 123

Query: 121 YPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNG 180
           YPKSFRDLVG NGVITAQGEQQRKLH IASN MR++   F FL++IQ III +L +FH+ 
Sbjct: 124 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFLKEIQTIIIHSLTTFHDR 183

Query: 181 -QLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRA 240
            Q+I LQD CRKIAIN+MVSQ+LGVSS+SEV EISGLFYDFVDGCLS PIN P FAYY A
Sbjct: 184 HQIISLQDACRKIAINVMVSQLLGVSSDSEVNEISGLFYDFVDGCLSFPINLPPFAYYSA 243

Query: 241 MKAREQIIGKIKKKIAWHKA--RMAEGRS--GEGMLGGVLEDQK-LSDEAVADFIINLLF 300
           MKAR+QII KIKKK+   K+  RM+   S  G G+L  +LE+QK L DEAV DFIINLLF
Sbjct: 244 MKARKQIIRKIKKKMKMQKSMERMSSSSSSSGHGVLARLLEEQKHLCDEAVEDFIINLLF 303

Query: 301 AGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDE 360
           AGNETTSKTMLFAVY+LTHSP A  QL+DEQ+GL N+ GD+TITWQDYKSM FTQCVIDE
Sbjct: 304 AGNETTSKTMLFAVYYLTHSPKAFNQLLDEQDGLRNRCGDNTITWQDYKSMSFTQCVIDE 363

Query: 361 TLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMEL 420
           TLRLGGIAIWLMRE KEEIKYSDYVIPKG FVVPFLSAVHLDE IY EA TFNPWRWM+L
Sbjct: 364 TLRLGGIAIWLMRETKEEIKYSDYVIPKGTFVVPFLSAVHLDENIYDEALTFNPWRWMQL 423

Query: 421 KNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
           +N+E RNW+SSP+FAPFGGGARLCPGAELARLQIALFLHHFVT YR
Sbjct: 424 QNQEKRNWKSSPYFAPFGGGARLCPGAELARLQIALFLHHFVTNYR 468

BLAST of CmaCh08G013100 vs. NCBI nr
Match: gi|700190426|gb|KGN45630.1| (hypothetical protein Csa_6G001760 [Cucumis sativus])

HSP 1 Score: 672.5 bits (1734), Expect = 5.6e-190
Identity = 338/462 (73.16%), Postives = 378/462 (81.82%), Query Frame = 1

Query: 3   MWEAS--PWSLLCVLTGLYALVLVARTRRKR----------KARAYRFPPGRRSWPLIGD 62
           MWE S   WS   +LT L+   L    ++K+              Y+FP GRRSWP+IGD
Sbjct: 16  MWEPSLVAWSFF-ILTLLFTTFLPNILKKKKYNDQNDQNDQSRTRYKFPSGRRSWPVIGD 75

Query: 63  SLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSS 122
           S  WYSA+ASSHP KYVEEQAKRYGKIFSCRVFGK  VVSVDP FNRYVMQNEG LF+SS
Sbjct: 76  SFNWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPDFNRYVMQNEGILFQSS 135

Query: 123 YPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNG 182
           YPKSFRDLVG NGVITAQGEQQRKLH IASN MR++   F F+++IQ III +L +FH+ 
Sbjct: 136 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFIKEIQTIIIHSLTTFHDR 195

Query: 183 -QLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRA 242
            Q+I LQD CRKIAINLMVSQ+LGVSS+SEV EISGLFYDFVDGCLS PIN P FAY+ A
Sbjct: 196 HQIISLQDACRKIAINLMVSQLLGVSSDSEVNEISGLFYDFVDGCLSFPINLPPFAYHSA 255

Query: 243 MKAREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLEDQK-LSDEAVADFIINLLFAGNE 302
           MKAR+QII KIKKKI   K+      SG G+LG +LE+QK L DEAV DFIINLLFAGNE
Sbjct: 256 MKARKQIIRKIKKKIEMQKSMEKSSSSGHGVLGRLLEEQKHLCDEAVEDFIINLLFAGNE 315

Query: 303 TTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDHTITWQDYKSMPFTQCVIDETLRL 362
           TTSKTMLFAVY+LTHSP A +QL++EQ+GL NK GD+T+TW+DYKSM FTQCVIDETLRL
Sbjct: 316 TTSKTMLFAVYYLTHSPKAFEQLMEEQDGLRNKCGDNTVTWEDYKSMSFTQCVIDETLRL 375

Query: 363 GGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHLDETIYGEASTFNPWRWMELKNEE 422
           GGIAIWLMRE KEEIKYSDYVIPKG FVVPFLSAVHLDE IY EA TFNPWRWM+L+N+E
Sbjct: 376 GGIAIWLMRETKEEIKYSDYVIPKGTFVVPFLSAVHLDENIYDEALTFNPWRWMQLQNQE 435

Query: 423 MRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHFVTKYR 451
            RNW+SSP+FAPFGGG RLCPGAELARLQIALFLHHF+T YR
Sbjct: 436 KRNWKSSPYFAPFGGGGRLCPGAELARLQIALFLHHFLTNYR 476

BLAST of CmaCh08G013100 vs. NCBI nr
Match: gi|778709023|ref|XP_011656332.1| (PREDICTED: LOW QUALITY PROTEIN: abietadienol/abietadienal oxidase [Cucumis sativus])

HSP 1 Score: 659.4 bits (1700), Expect = 4.9e-186
Identity = 338/485 (69.69%), Postives = 378/485 (77.94%), Query Frame = 1

Query: 3   MWEAS--PWSLLCVLTGLYALVLVARTRRKR----------KARAYRFPPGRRSWPLIGD 62
           MWE S   WS   +LT L+   L    ++K+              Y+FP GRRSWP+IGD
Sbjct: 1   MWEPSLVAWSFF-ILTLLFTTFLPNILKKKKYNDQNDQNDQSRTRYKFPSGRRSWPVIGD 60

Query: 63  SLKWYSAVASSHPPKYVEEQAKRYGKIFSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSS 122
           S  WYSA+ASSHP KYVEEQAKRYGKIFSCRVFGK  VVSVDP FNRYVMQNEG LF+SS
Sbjct: 61  SFNWYSAIASSHPSKYVEEQAKRYGKIFSCRVFGKCTVVSVDPDFNRYVMQNEGILFQSS 120

Query: 123 YPKSFRDLVGTNGVITAQGEQQRKLHAIASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNG 182
           YPKSFRDLVG NGVITAQGEQQRKLH IASN MR++   F F+++IQ III +L +FH+ 
Sbjct: 121 YPKSFRDLVGVNGVITAQGEQQRKLHGIASNFMRINKLNFSFIKEIQTIIIHSLTTFHDR 180

Query: 183 -QLICLQDVCRKIAINLMVSQVLGVSSESEVEEISGLFYDFVDGCLSIPINFPGFAYYRA 242
            Q+I LQD CRKIAINLMVSQ+LGVSS+SEV EISGLFYDFVDGCLS PIN P FAY+ A
Sbjct: 181 HQIISLQDACRKIAINLMVSQLLGVSSDSEVNEISGLFYDFVDGCLSFPINLPPFAYHSA 240

Query: 243 MK-----------------------AREQIIGKIKKKIAWHKARMAEGRSGEGMLGGVLE 302
           MK                       AR+QII KIKKKI   K+      SG G+LG +LE
Sbjct: 241 MKVXSITIVIIAXYRIIFLILCIVXARKQIIRKIKKKIEMQKSMEKSSSSGHGVLGRLLE 300

Query: 303 DQK-LSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEGLTNKNGDH 362
           +QK L DEAV DFIINLLFAGNETTSKTMLFAVY+LTHSP A +QL++EQ+GL NK GD+
Sbjct: 301 EQKHLCDEAVEDFIINLLFAGNETTSKTMLFAVYYLTHSPKAFEQLMEEQDGLRNKCGDN 360

Query: 363 TITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVVPFLSAVHL 422
           T+TW+DYKSM FTQCVIDETLRLGGIAIWLMRE KEEIKYSDYVIPKG FVVPFLSAVHL
Sbjct: 361 TVTWEDYKSMSFTQCVIDETLRLGGIAIWLMRETKEEIKYSDYVIPKGTFVVPFLSAVHL 420

Query: 423 DETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQIALFLHHF 451
           DE IY EA TFNPWRWM+L+N+E RNW+SSP+FAPFGGG RLCPGAELARLQIALFLHHF
Sbjct: 421 DENIYDEALTFNPWRWMQLQNQEKRNWKSSPYFAPFGGGGRLCPGAELARLQIALFLHHF 480

BLAST of CmaCh08G013100 vs. NCBI nr
Match: gi|1021521306|ref|XP_016204040.1| (PREDICTED: abietadienol/abietadienal oxidase [Arachis ipaensis])

HSP 1 Score: 654.8 bits (1688), Expect = 1.2e-184
Identity = 311/433 (71.82%), Postives = 363/433 (83.83%), Query Frame = 1

Query: 18  LYALVLVARTRRKRKARAYRFPPGRRSWPLIGDSLKWYSAVASSHPPKYVEEQAKRYGKI 77
           ++A ++  + ++  K    R PPGRR WPLIGDS+ WY+AVASSHPP++VEE  KRYGKI
Sbjct: 25  MFAKMVQEKLKKGEKRSKLRLPPGRRGWPLIGDSINWYNAVASSHPPQFVEEMVKRYGKI 84

Query: 78  FSCRVFGKWAVVSVDPVFNRYVMQNEGKLFKSSYPKSFRDLVGTNGVITAQGEQQRKLHA 137
           FSC +FGKWAVVS DP FNR+VMQNEGKLF+SSYPKSFRDLVG NGVIT QGEQQRKLH 
Sbjct: 85  FSCSLFGKWAVVSADPTFNRFVMQNEGKLFRSSYPKSFRDLVGKNGVITVQGEQQRKLHG 144

Query: 138 IASNMMRLHDPKFQFLRDIQIIIIQTLGSFHNGQLICLQDVCRKIAINLMVSQVLGVSSE 197
           IASNMMRL   KF FL DIQ +++QTL +F N Q+I LQDVCRK+AINLMV+Q+LGVSSE
Sbjct: 145 IASNMMRLEKLKFHFLNDIQKVMLQTLSNFENNQVILLQDVCRKVAINLMVNQLLGVSSE 204

Query: 198 SEVEEISGLFYDFVDGCLSIPINFPGFAYYRAMKAREQIIGKIKKKIAWHKARMAEGRSG 257
           S++ E++ LF DFVDGCLSIP+N PGFAY+ AMKARE+II KI K I  H+   A    G
Sbjct: 205 SQINEMAQLFSDFVDGCLSIPVNTPGFAYHTAMKAREKIINKINKTIEVHRKNDAP-TVG 264

Query: 258 EGMLGGVLEDQKLSDEAVADFIINLLFAGNETTSKTMLFAVYFLTHSPTAMKQLVDEQEG 317
            G+LG +LE++ L D+AVADFIINLLFAGNETT+KTMLFAV+FLT  P AMKQL+DEQ+ 
Sbjct: 265 SGVLGRLLEEESLPDDAVADFIINLLFAGNETTTKTMLFAVFFLTQCPQAMKQLLDEQDS 324

Query: 318 LTNKNGDHTITWQDYKSMPFTQCVIDETLRLGGIAIWLMREAKEEIKYSDYVIPKGCFVV 377
           L + +G+  +TWQDYK+MPFTQCVIDETLRLGGIAIWLMREAKE+I+Y D+VIPKGCFVV
Sbjct: 325 LRSNSGEEFLTWQDYKAMPFTQCVIDETLRLGGIAIWLMREAKEDIQYQDFVIPKGCFVV 384

Query: 378 PFLSAVHLDETIYGEASTFNPWRWMELKNEEMRNWRSSPFFAPFGGGARLCPGAELARLQ 437
           PFLSAVHLDE +Y  A  FNPWRWME +NEE RNWR+SPF+APFGGGAR CPGAELARLQ
Sbjct: 385 PFLSAVHLDENVYNGALNFNPWRWMEPENEEKRNWRTSPFYAPFGGGARFCPGAELARLQ 444

Query: 438 IALFLHHFVTKYR 451
           IALFLH+FVT YR
Sbjct: 445 IALFLHYFVTTYR 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C72B1_PINTA1.5e-10243.36Abietadienol/abietadienal oxidase OS=Pinus taeda GN=CYP720B1 PE=1 SV=1[more]
C72B2_PINTA3.6e-9641.81Cytochrome P450 720B2 OS=Pinus taeda GN=CYP720B2 PE=2 SV=1[more]
C90B1_ARATH7.2e-8133.41Cytochrome P450 90B1 OS=Arabidopsis thaliana GN=CYP90B1 PE=1 SV=2[more]
C90D1_ARATH2.9e-7432.783-epi-6-deoxocathasterone 23-monooxygenase OS=Arabidopsis thaliana GN=CYP90D1 PE... [more]
C90D2_ORYSJ5.0e-7432.46Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica GN=CYP90D2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K7D5_CUCSA3.9e-19073.16Uncharacterized protein OS=Cucumis sativus GN=Csa_6G001760 PE=3 SV=1[more]
V7C555_PHAVU2.4e-18471.85Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G136300g PE=3 SV=1[more]
A0A0L9VHS8_PHAAN2.7e-18371.40Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan10g051700 PE=3 SV=1[more]
A0A0S3TBJ6_PHAAN2.7e-18371.40Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G209500 PE=... [more]
A0A072UB54_MEDTR1.0e-18270.23Cytochrome P450 family protein OS=Medicago truncatula GN=MTR_6g069000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73340.11.4e-16260.13 Cytochrome P450 superfamily protein[more]
AT3G50660.14.1e-8233.41 Cytochrome P450 superfamily protein[more]
AT3G13730.11.7e-7532.78 cytochrome P450, family 90, subfamily D, polypeptide 1[more]
AT4G36380.11.1e-7433.65 Cytochrome P450 superfamily protein[more]
AT5G05690.17.0e-7435.28 Cytochrome P450 superfamily protein[more]
Match NameE-valueIdentityDescription
gi|659116813|ref|XP_008458272.1|1.1e-19074.25PREDICTED: abietadienol/abietadienal oxidase isoform X2 [Cucumis melo][more]
gi|659116811|ref|XP_008458271.1|1.1e-19074.25PREDICTED: abietadienol/abietadienal oxidase isoform X1 [Cucumis melo][more]
gi|700190426|gb|KGN45630.1|5.6e-19073.16hypothetical protein Csa_6G001760 [Cucumis sativus][more]
gi|778709023|ref|XP_011656332.1|4.9e-18669.69PREDICTED: LOW QUALITY PROTEIN: abietadienol/abietadienal oxidase [Cucumis sativ... [more]
gi|1021521306|ref|XP_016204040.1|1.2e-18471.82PREDICTED: abietadienol/abietadienal oxidase [Arachis ipaensis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002403Cyt_P450_E_grp-IV
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
GO:0004497monooxygenase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G013100.1CmaCh08G013100.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 419..428
score: 2.8E-10coord: 285..302
score: 2.8E-10coord: 340..351
score: 2.8E-10coord: 428..439
score: 2.8
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 19..452
score: 1.4
IPR001128Cytochrome P450PFAMPF00067p450coord: 39..453
score: 1.2
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 34..453
score: 4.98
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 368..382
score: 1.6E-21coord: 412..428
score: 1.6E-21coord: 335..351
score: 1.6E-21coord: 428..446
score: 1.6E-21coord: 276..302
score: 1.6E-21coord: 65..88
score: 1.6E-21coord: 384..402
score: 1.6
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 421..430
scor
NoneNo IPR availablePANTHERPTHR24286FAMILY NOT NAMEDcoord: 7..450
score: 1.4E
NoneNo IPR availablePANTHERPTHR24286:SF83CYTOCHROME P450 SUPERFAMILY PROTEINcoord: 7..450
score: 1.4E

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None