CmaCh08G009070 (gene) Cucurbita maxima (Rimu)

NameCmaCh08G009070
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionRop guanine nucleotide exchange factor 2
LocationCma_Chr08 : 5633577 .. 5635998 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTTTCTCTGGTCCTAAAGTTGCAGAGCTGCTTCCCTCTCTTTTTCCAAAAGAAACAAAAAGAAATTTCAGAGAAAGAAAAAGGGAAAAGAAAGCAACCCACAAGGAGGGAATAGCAATTCAAAAATCCTTTAACTCTCTTCTTCTTCTTCTTCAAAATTCAAATTTATTAACCAAACCAGAGCTTCCAATGGAGAAATTCTCCATCTCCGACCAAAAATTTCACCTGGGTTTCTGCCCTTCCTCCTCTTCCATGGATCTAAATGACCCATCCACCGTTTCTGAAACTTCCGGCCAATCTCCGCCCAGCCTCGCCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCTGCCTTCTCCGACCCCACCACCGACGATACCAGTTTCTGCACTGAGCCCTCTCCAGCGATTCCCACCAGAAAATATAAACTCAACGCCGGAGATAAAGCCGACGATCAAGACGCCATGGATTCCGGTGAGTGGGTTTTGTGAATTTTCTCCCCCTTCTTACAAAATCACAAGTTTTTTTTTTAACATTGTTGCAGAAACAGAGATGATGAAGGAGAGATTTGCGAAGCTTTTGCTCGGAGAAGACATGTCCGGAAGTGGGAAAGGGGTTTGCACGGCGGTTACAATCTCAAACGCCATTACTAATCTCTATGGTCAGATTTTTCTTTTTCCGAACTGTTCTAACTTTTTTTGATTCTGGGTCTTAACTTTTGGCTCTGTTTTTTGGTCTGATCAGCGACTGTGTTCGGACAGAATCTGAAACTGGAGCCGTTGCCGGCGGAGAAGAAGGCGATGTGGAAGAGAGAAATGGCTTGTCTGTTATCTGTTTGTGATTACATTCTTGAGTTCTTCACTGAATCTCAAACCTTGGACGATGGAACAACAATGGAGGTCACATTTCTCTGTCCTTTTCAGAACAAAATTGGTTCTGTTTTAGAACAAAATTGTCCGTCGGCTAGGATGTCGGTTTTAATGATGTTTTTTTTTTCAGGTTTTGAACAGCCGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGGAAAAGCTTGACATGATGCTCTTGGTAAATATTATTAATAACTTTGTAATTGAACTTCGGTTGAATATTTTGAGAGTGATCCATCAATCTTGTTTGGTTTTGGGTTGGCAGGATATTTTGGATAGTTTTCACGACACGGAGTTTTGGTATGCTGAAAAAGGAAGTATGTCGTCGAACTCGAACTCGAATCGTTCGAAATCGGCTTCGTTTCGCCGACTTTCGCTGCAGAAAGAAGAGAAATGGTGGTTACCAGTTCCTTGCCTTCCTTCATGTGGTCTATCAGAGAAAGCACGAAAACAACTAAGAAATAATCGTGAATTTGCGAATCAAATCCATAAAGCTGCTATGGCTATTAACAGTACTATTCTTGCTGAAATGGATATACCTGAGTCATACATCGACACTCTTCCAAAGGTTAGTCCTCCAACCAATCGTAGGAAGATAGCAATCTTTACTTTCGACCAGTGTAAATCCGTTTTCTGATTGGTTTTCACAAAACAACCCATTTTTCATTTTTTCAGAGTGGAAAAACGAGTATAGGCGATACGATGTACCGTTATATGTACAGTGCTGACAAATTCTCGCCCGATCATTTGCTCGATTGCTTGAACATAGGATCTGAACACGATGCGCTCGAACTTGCAGACCGGATTGAGGCTTCGATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCATCGTGGGAATTGGTAAAAGATCTAATGGCTGAAGGTGATAAAGGTGACAAGAACACTATCTTAGCTGAAAGAGCCGAGAGCTTGCTTTTGTCCTTGAAACAACGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGTAAGATTCAATACAACAGGGTAAGTTAAGAGTATATGTCTTGTCCACTTACGCTCTTCTCGATTTTTGAATTTTTAAAAGTGCATGTTAGATACAAAATTGAAAGTTTATGGATCAAATATGCGTTAAATTAAAAGTTTAAAGATCTATTAAACCCATTTTAGAATCCCGATGCTTATAAGATTTGATGTTGTAGGACGTGGGACAAGCAGTGTTGGAGAGTTATTCGAGGGTGTTGGAAGGACTGGCATTTAACGTTGTGGCATGGATTGAAGATGTTCTTTTTGCTGATAGGTCCCTATGAAACCAAGAGTAGGATCTCCTCTCTTACCAAGAAAAGGAAATGTTTAGTTGTTTGTTTATGTTGTACGGTCACAATTTGTATAAAAACTATGTTGTTAGGTTGGGACAAAGGGTTCCTCCCTATTTGTAGAATGTATTTATCTTTTAGGTTAAAGTTACACGGAAAATTGTACCTTTTTTTTTTAATCGGTTTGATTGAAAAGGCATAGTCACTTACC

mRNA sequence

TGTTTTCTCTGGTCCTAAAGTTGCAGAGCTGCTTCCCTCTCTTTTTCCAAAAGAAACAAAAAGAAATTTCAGAGAAAGAAAAAGGGAAAAGAAAGCAACCCACAAGGAGGGAATAGCAATTCAAAAATCCTTTAACTCTCTTCTTCTTCTTCTTCAAAATTCAAATTTATTAACCAAACCAGAGCTTCCAATGGAGAAATTCTCCATCTCCGACCAAAAATTTCACCTGGGTTTCTGCCCTTCCTCCTCTTCCATGGATCTAAATGACCCATCCACCGTTTCTGAAACTTCCGGCCAATCTCCGCCCAGCCTCGCCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCTGCCTTCTCCGACCCCACCACCGACGATACCAGTTTCTGCACTGAGCCCTCTCCAGCGATTCCCACCAGAAAATATAAACTCAACGCCGGAGATAAAGCCGACGATCAAGACGCCATGGATTCCGAAACAGAGATGATGAAGGAGAGATTTGCGAAGCTTTTGCTCGGAGAAGACATGTCCGGAAGTGGGAAAGGGGTTTGCACGGCGGTTACAATCTCAAACGCCATTACTAATCTCTATGCGACTGTGTTCGGACAGAATCTGAAACTGGAGCCGTTGCCGGCGGAGAAGAAGGCGATGTGGAAGAGAGAAATGGCTTGTCTGTTATCTGTTTGTGATTACATTCTTGAGTTCTTCACTGAATCTCAAACCTTGGACGATGGAACAACAATGGAGGTTTTGAACAGCCGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGGAAAAGCTTGACATGATGCTCTTGGATATTTTGGATAGTTTTCACGACACGGAGTTTTGGTATGCTGAAAAAGGAAGTATGTCGTCGAACTCGAACTCGAATCGTTCGAAATCGGCTTCGTTTCGCCGACTTTCGCTGCAGAAAGAAGAGAAATGGTGGTTACCAGTTCCTTGCCTTCCTTCATGTGGTCTATCAGAGAAAGCACGAAAACAACTAAGAAATAATCGTGAATTTGCGAATCAAATCCATAAAGCTGCTATGGCTATTAACAGTACTATTCTTGCTGAAATGGATATACCTGAGTCATACATCGACACTCTTCCAAAGAGTGGAAAAACGAGTATAGGCGATACGATGTACCGTTATATGTACAGTGCTGACAAATTCTCGCCCGATCATTTGCTCGATTGCTTGAACATAGGATCTGAACACGATGCGCTCGAACTTGCAGACCGGATTGAGGCTTCGATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCATCGTGGGAATTGGTAAAAGATCTAATGGCTGAAGGTGATAAAGGTGACAAGAACACTATCTTAGCTGAAAGAGCCGAGAGCTTGCTTTTGTCCTTGAAACAACGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGTAAGATTCAATACAACAGGGTAAGTTAAGAGTATATGTCTTGTCCACTTACGCTCTTCTCGATTTTTGAATTTTTAAAAGTGCATGTTAGATACAAAATTGAAAGTTTATGGATCAAATATGCGTTAAATTAAAAGTTTAAAGATCTATTAAACCCATTTTAGAATCCCGATGCTTATAAGATTTGATGTTGTAGGACGTGGGACAAGCAGTGTTGGAGAGTTATTCGAGGGTGTTGGAAGGACTGGCATTTAACGTTGTGGCATGGATTGAAGATGTTCTTTTTGCTGATAGGTCCCTATGAAACCAAGAGTAGGATCTCCTCTCTTACCAAGAAAAGGAAATGTTTAGTTGTTTGTTTATGTTGTACGGTCACAATTTGTATAAAAACTATGTTGTTAGGTTGGGACAAAGGGTTCCTCCCTATTTGTAGAATGTATTTATCTTTTAGGTTAAAGTTACACGGAAAATTGTACCTTTTTTTTTTAATCGGTTTGATTGAAAAGGCATAGTCACTTACC

Coding sequence (CDS)

ATGGAGAAATTCTCCATCTCCGACCAAAAATTTCACCTGGGTTTCTGCCCTTCCTCCTCTTCCATGGATCTAAATGACCCATCCACCGTTTCTGAAACTTCCGGCCAATCTCCGCCCAGCCTCGCCTACTCTTTCTCTTACTGCAGAACCAGCTCCGACGCCTCTGCCTTCTCCGACCCCACCACCGACGATACCAGTTTCTGCACTGAGCCCTCTCCAGCGATTCCCACCAGAAAATATAAACTCAACGCCGGAGATAAAGCCGACGATCAAGACGCCATGGATTCCGAAACAGAGATGATGAAGGAGAGATTTGCGAAGCTTTTGCTCGGAGAAGACATGTCCGGAAGTGGGAAAGGGGTTTGCACGGCGGTTACAATCTCAAACGCCATTACTAATCTCTATGCGACTGTGTTCGGACAGAATCTGAAACTGGAGCCGTTGCCGGCGGAGAAGAAGGCGATGTGGAAGAGAGAAATGGCTTGTCTGTTATCTGTTTGTGATTACATTCTTGAGTTCTTCACTGAATCTCAAACCTTGGACGATGGAACAACAATGGAGGTTTTGAACAGCCGACAAAGGTCAGATATATACCTAAACCTTCCAGCATTGGAAAAGCTTGACATGATGCTCTTGGATATTTTGGATAGTTTTCACGACACGGAGTTTTGGTATGCTGAAAAAGGAAGTATGTCGTCGAACTCGAACTCGAATCGTTCGAAATCGGCTTCGTTTCGCCGACTTTCGCTGCAGAAAGAAGAGAAATGGTGGTTACCAGTTCCTTGCCTTCCTTCATGTGGTCTATCAGAGAAAGCACGAAAACAACTAAGAAATAATCGTGAATTTGCGAATCAAATCCATAAAGCTGCTATGGCTATTAACAGTACTATTCTTGCTGAAATGGATATACCTGAGTCATACATCGACACTCTTCCAAAGAGTGGAAAAACGAGTATAGGCGATACGATGTACCGTTATATGTACAGTGCTGACAAATTCTCGCCCGATCATTTGCTCGATTGCTTGAACATAGGATCTGAACACGATGCGCTCGAACTTGCAGACCGGATTGAGGCTTCGATGTATACATGGAGAAGGAAAGCATGTCTAAGCCACTCAAAATCATCGTGGGAATTGGTAAAAGATCTAATGGCTGAAGGTGATAAAGGTGACAAGAACACTATCTTAGCTGAAAGAGCCGAGAGCTTGCTTTTGTCCTTGAAACAACGGTTCCCTGAACTTTCTCAAACAACCTTAGACACTAGTAAGATTCAATACAACAGGGTAAGTTAA

Protein sequence

MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDPTTDDTSFCTEPSPAIPTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTSKIQYNRVS
BLAST of CmaCh08G009070 vs. Swiss-Prot
Match: ROGF3_ARATH (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 9.4e-138
Identity = 264/424 (62.26%), Postives = 325/424 (76.65%), Query Frame = 1

Query: 18  SSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDA-SAFSDPTTDDTSFCTEPSPA-- 77
           S  S+D ND S V ET   S  S+  SF Y RT S+  S FSD   +  SFC+E SP   
Sbjct: 15  SPRSIDQNDQSAV-ETPVYSTMSID-SFVYPRTCSETTSGFSDQIDETNSFCSEASPCNW 74

Query: 78  ---IPTRKYKLNAGDKADD------QDAMDSETEMMKERFAKLLLGEDMSGSGKGVCTAV 137
                ++  K  +G +         Q+  + E E MKERFAKLLLGEDMSGSGKGVCTAV
Sbjct: 75  PVLTESKSSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAV 134

Query: 138 TISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDDGTT 197
           TISNAITNLYATVFGQNL+LEPL  EK+A+WKREM CLLSVCDYI+EF    Q L +G T
Sbjct: 135 TISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGAT 194

Query: 198 MEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKSASF 257
           +EV+ SR R+DIY+NLPAL KLD ML++ LDSF +TEFWYAE+GS+S  S   RS + SF
Sbjct: 195 VEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSA--RSSTGSF 254

Query: 258 RRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMDIP 317
           R++ +Q KEEKWWLPVP +PS GLS+KARKQL+N RE  NQIHKAAMAINS+IL+EM+IP
Sbjct: 255 RKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIP 314

Query: 318 ESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMYTW 377
           +SY+ TLPK GK+S+GD++YRYM  + +F P+ LLDCLNI SEH+A++LADR+EASMYTW
Sbjct: 315 DSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTW 374

Query: 378 RRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTSKI 429
           RRK+CLS+SK+SW +VKDLM+  ++ DKN ++AERAE+LL  LKQR+PELSQT+LD  KI
Sbjct: 375 RRKSCLSNSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKI 434

BLAST of CmaCh08G009070 vs. Swiss-Prot
Match: ROGF2_ARATH (Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana GN=ROPGEF2 PE=1 SV=2)

HSP 1 Score: 480.7 bits (1236), Expect = 1.7e-134
Identity = 267/450 (59.33%), Postives = 325/450 (72.22%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASA--FS 60
           ME     ++   +G+  S   +D ND S  SET   S  S   SF+Y RT S+ S   FS
Sbjct: 1   MENLPNHEENDDVGYHQSPGPIDPNDHSA-SETPVYSTMSTD-SFAYHRTCSETSGGGFS 60

Query: 61  DPTTDDTSFCTEPSPA---------------IPT----RKYKLNAGDKADDQDAMDSETE 120
           D   + +SFCTE SP+                PT    +  ++   +    Q+  + E E
Sbjct: 61  DQIDETSSFCTEASPSDWPVLTESNNSASSNFPTVFDLKHNQIETDEHLAVQEISEPELE 120

Query: 121 MMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKRE 180
            MKERF+KLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  E+K  WKRE
Sbjct: 121 TMKERFSKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLEIEQKTTWKRE 180

Query: 181 MACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFH 240
           M CLLSVCDYI EF  +SQ L +G T+EV+ SR R+DIY+NLPAL KLD ML++ LDSF 
Sbjct: 181 MNCLLSVCDYIFEFIPKSQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQ 240

Query: 241 DTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRN 300
            TEFWYAE+GS+S    S RS + SFR++ +Q KEEKWWLP+P +P  GLSEKARKQL++
Sbjct: 241 KTEFWYAEEGSLS--MKSTRSATGSFRKVIVQRKEEKWWLPIPLVPLQGLSEKARKQLKS 300

Query: 301 NREFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHL 360
            RE  NQIHKAAMAINS+IL EMDIP+SY+ TLPKSGK S GD +YR+M S+ +FSP+ L
Sbjct: 301 KRESTNQIHKAAMAINSSILGEMDIPDSYMATLPKSGKASTGDAIYRHMTSSGRFSPEKL 360

Query: 361 LDCLNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAE 420
           LD L I SEH+AL+LADR+EASMYTWRRKACL++SKSSW +VKDLM+  ++ DKN +LAE
Sbjct: 361 LDRLKIVSEHEALQLADRVEASMYTWRRKACLNNSKSSWNMVKDLMSITERSDKNYVLAE 420

Query: 421 RAESLLLSLKQRFPELSQTTLDTSKIQYNR 429
           RAESLL  LKQR+PELSQT+LD  KI  N+
Sbjct: 421 RAESLLFCLKQRYPELSQTSLDICKIHCNK 446

BLAST of CmaCh08G009070 vs. Swiss-Prot
Match: ROGF4_ARATH (Rop guanine nucleotide exchange factor 4 OS=Arabidopsis thaliana GN=ROPGEF4 PE=1 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 4.1e-117
Identity = 233/431 (54.06%), Postives = 302/431 (70.07%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           ME  S SDQ         +SS+      T S+ SG S      SF    ++++A   S  
Sbjct: 1   MESSSNSDQNEGT----PTSSVSSPYRRTYSDISGLSHRFDVQSFYNRPSNTNAVVLSGH 60

Query: 61  TTDDTSFCTEPSPAIPTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKG 120
             D +    EP   +    +     + +D   A D+E EMM+ERFAKLLLGEDMSGSGKG
Sbjct: 61  EEDVSEDAEEPKDDVVNDVHGDGDEEDSDIDSAEDAELEMMRERFAKLLLGEDMSGSGKG 120

Query: 121 VCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTL 180
           VCTAVT+SN+ITNLYATVFGQ+L+L+PL  EKK +WKREM C +S+CDYI+E    S   
Sbjct: 121 VCTAVTVSNSITNLYATVFGQSLRLQPLSTEKKDLWKREMNCFMSICDYIVEVIPRSL-- 180

Query: 181 DDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRS 240
             GT +E+  ++ RSDI ++LPAL KLD ML++ILDSF + EFWY E+GS S NS     
Sbjct: 181 --GTNVEITETKLRSDILMSLPALRKLDNMLMEILDSFTENEFWYVERGSSSMNSGGGGR 240

Query: 241 KSASFRRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILA 300
            S +FR++ +Q K+EKWWLPVPC+P+ GLSE+ RK LR+ R+ A+QIHKAA+AIN + L 
Sbjct: 241 DSGTFRKVVVQRKDEKWWLPVPCVPAEGLSEEERKHLRHKRDCASQIHKAALAINDSTLN 300

Query: 301 EMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEA 360
           +MDIP+SY+ TLPKSGK S+GD +Y+ + +A+KF PD LLD L I SEH+ALELAD++EA
Sbjct: 301 DMDIPDSYLTTLPKSGKASVGDVIYKQLCTAEKFYPDQLLDILKITSEHEALELADKVEA 360

Query: 361 SMYTWRRK-ACLSHSKSSWELVKDLMAEGDKG-DKNTILAERAESLLLSLKQRFPELSQT 420
           S+ TWRRK   L+HSKSSW+++KD+  + D+G DKN ILA RA SLL  LKQR+PELSQT
Sbjct: 361 SLVTWRRKTGGLTHSKSSWDMMKDISGDADRGNDKNHILAARARSLLFCLKQRYPELSQT 420

Query: 421 TLDTSKIQYNR 429
           +LD  KIQ+NR
Sbjct: 421 SLDICKIQFNR 423

BLAST of CmaCh08G009070 vs. Swiss-Prot
Match: ROGF7_ARATH (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 2.5e-106
Identity = 208/388 (53.61%), Postives = 280/388 (72.16%), Query Frame = 1

Query: 53  DASAFSDPTT-----DDTSFCTEPSPAIPTRKY----KLNAGDKADDQDAMDSETEMMKE 112
           ++S  S  TT     + +  CTE   A P         ++   K    D+  SE EMMKE
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEKKKIRSDSRVSEVEMMKE 76

Query: 113 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACL 172
           RF+KLLLGEDMSGSG GVCTA+ ISNAITNL AT+FGQ  +LEPLP EKK MW+REM  L
Sbjct: 77  RFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKEMWRREMEWL 136

Query: 173 LSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEF 232
           L V D+I+E     QT  DGT +E++  R RSD+Y+NLPAL KLD MLL+ILDSF +TEF
Sbjct: 137 LCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEILDSFEETEF 196

Query: 233 WYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFA 292
           WY ++G M+  S ++   S+SFR+   ++E+KWWLPVP +   GL E +RKQL++ R+  
Sbjct: 197 WYVDQGIMAHESAAD--GSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQLQHKRDCT 256

Query: 293 NQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLN 352
           NQI KAAMAINS  LA+M+IPESY+++LP+ G++ +GD +YRY+ S+D+FSP+ LLDCL+
Sbjct: 257 NQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYI-SSDQFSPECLLDCLD 316

Query: 353 IGSEHDALELADRIEASMYTWRRKA---CLSHSKSSWELVKDLMAEGDKGDKNTILAERA 412
           + SEH A+E+A+R+E+S+Y W ++      +++K+SWE+VK+LM + DK +   ++A+RA
Sbjct: 317 LSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLE---LMADRA 376

Query: 413 ESLLLSLKQRFPELSQTTLDTSKIQYNR 429
           ESLLLSLKQRFP L QT LD SKIQYN+
Sbjct: 377 ESLLLSLKQRFPGLPQTALDMSKIQYNK 398

BLAST of CmaCh08G009070 vs. Swiss-Prot
Match: ROGF5_ARATH (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 6.3e-102
Identity = 211/421 (50.12%), Postives = 283/421 (67.22%), Query Frame = 1

Query: 18  SSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDPTTDDTSFCTEPSPAIPT 77
           S +S + +  +TV+ +S   PPS    +   + S   ++      D              
Sbjct: 36  SGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESVNFDHKKLTLHD------ 95

Query: 78  RKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYAT 137
                ++G KA + ++ D   EMMKERFAKLLLGEDMSGSGKGVCTA+ ISNAITNL AT
Sbjct: 96  -----DSGFKAKEMNSAD--VEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLCAT 155

Query: 138 VFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDI 197
           +FGQ  +LEPL +EKK MW+REM  +LSV D+I+E    +QT  DG   EV+  R R D+
Sbjct: 156 IFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRFDL 215

Query: 198 YLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWW 257
           ++NLPAL KLD MLLDIL SF  TEFWY ++G ++S ++     SASFRR   ++EEKWW
Sbjct: 216 FINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDG----SASFRRKIQRQEEKWW 275

Query: 258 LPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKT 317
           LPVP L   GL+E+AR +L + RE A QI KAAMAINS  L EMD+P+SY++TLPK+G++
Sbjct: 276 LPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRS 335

Query: 318 SIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMYTWRRK---------- 377
            +GD +YRY+ ++DKFS + LLDCL++ SEH AL++A+R+EAS+Y WRR+          
Sbjct: 336 CLGDVIYRYV-TSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNN 395

Query: 378 ACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTSKIQYN 429
              +  K +WE+VK+LMA    GDK  +L ER+E+LL  LKQRFP L+QT+LD SKIQ+N
Sbjct: 396 TSSTTPKLTWEMVKELMA---AGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWN 435

BLAST of CmaCh08G009070 vs. TrEMBL
Match: A0A0A0K849_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004490 PE=4 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 3.1e-212
Identity = 385/432 (89.12%), Postives = 402/432 (93.06%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+KFSISD+ FHLGF PSSSSMDLND ST+S+TSGQSP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPAS-GYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDTSFCTEPSPAIP----TRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSG 120
           TTDD SFCTEPSPAI     TRKYK   GDKADDQDAMDSE EMMKERFAKLLLGEDMSG
Sbjct: 61  TTDDGSFCTEPSPAILGRQCTRKYKHAVGDKADDQDAMDSEMEMMKERFAKLLLGEDMSG 120

Query: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTE 180
           SGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM+CLLSVCDYI+EFFT 
Sbjct: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTL 180

Query: 181 SQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSN 240
           SQTL+DGT++EVLNSRQRSDI LNLPAL+KLDMMLLDILDSF DTEFWYAEKGSMSSNSN
Sbjct: 181 SQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSMSSNSN 240

Query: 241 SNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINST 300
             RS+S SFRRLSLQKEEKWWLPVPC+PSCGLSE ARK LRN RE ANQIHKAAMAINST
Sbjct: 241 --RSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINST 300

Query: 301 ILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADR 360
           ILAEMDIPES+IDTLPKSGK SIGDT+YRYMYSADKFSP+HLLDCLNIGSEHDALELADR
Sbjct: 301 ILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADR 360

Query: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQ 420
           IEASMYTWRRKACLSHSKSSWELVKDLMAE +K DKNTILAERAE+LLLSLKQRFPELSQ
Sbjct: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQ 420

Query: 421 TTLDTSKIQYNR 429
           TTLDTSKIQYNR
Sbjct: 421 TTLDTSKIQYNR 429

BLAST of CmaCh08G009070 vs. TrEMBL
Match: A0A061DT39_THECC (RHO guanyl-nucleotide exchange factor 2 isoform 4 OS=Theobroma cacao GN=TCM_005331 PE=4 SV=1)

HSP 1 Score: 574.7 bits (1480), Expect = 9.4e-161
Identity = 299/446 (67.04%), Postives = 359/446 (80.49%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+  S SD+ + LG+ PS SS+D ND ST   T G SP S   SF YCRT S+ SAFSDP
Sbjct: 1   MDSMSNSDENYDLGYQPSPSSIDQNDQSTTG-TPGHSPLS-GDSFVYCRTYSETSAFSDP 60

Query: 61  TTDDTSFCTEPSPA-----------------IPTRKYKLNAGDKADDQDAMDSETEMMKE 120
             D+ S C+E SP+                 + T++ K NA DK DDQD++D E +MMKE
Sbjct: 61  V-DNHSCCSEASPSHWPPTRSGAQNQAVLSRLGTKQRKSNADDKLDDQDSLDLELDMMKE 120

Query: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACL 180
           RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  EKKA+WKREM CL
Sbjct: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLNPEKKALWKREMDCL 180

Query: 181 LSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEF 240
           LSVCDYI+EF ++SQ L +GT +E++ SRQRSDIY+NLPAL KLD ML++ILDSF D EF
Sbjct: 181 LSVCDYIVEFSSKSQNLCNGTAVEIMESRQRSDIYINLPALRKLDAMLIEILDSFQDREF 240

Query: 241 WYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFA 300
           WYAE+GSMSSNS    +++ SFRR+  + +EKWW+PVPC+P  GLSEKARK LR+ R+ A
Sbjct: 241 WYAEQGSMSSNS----TRTGSFRRVVQRNDEKWWVPVPCVPPTGLSEKARKCLRHKRDCA 300

Query: 301 NQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLN 360
           NQIHKAAMAINS +LAEM+IP+SY+ +LPKSG+ SIGD +YR+MY+ +KFSPD+LLDCLN
Sbjct: 301 NQIHKAAMAINSGVLAEMEIPDSYMASLPKSGRASIGDPIYRFMYTTEKFSPDYLLDCLN 360

Query: 361 IGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESL 420
           I SEH+ALELADR+EASMYTWRRKAC+SHSKSSW +VKDLM++ D+ DKN ILAERAESL
Sbjct: 361 IASEHEALELADRVEASMYTWRRKACMSHSKSSWGMVKDLMSDVDRSDKNHILAERAESL 420

Query: 421 LLSLKQRFPELSQTTLDTSKIQYNRV 430
           L +LKQR+PEL+QT+LDT KIQYNRV
Sbjct: 421 LFALKQRYPELAQTSLDTCKIQYNRV 439

BLAST of CmaCh08G009070 vs. TrEMBL
Match: A0A061DU87_THECC (RHO guanyl-nucleotide exchange factor 3 isoform 1 OS=Theobroma cacao GN=TCM_005331 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 3.6e-160
Identity = 298/445 (66.97%), Postives = 358/445 (80.45%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+  S SD+ + LG+ PS SS+D ND ST   T G SP S   SF YCRT S+ SAFSDP
Sbjct: 1   MDSMSNSDENYDLGYQPSPSSIDQNDQSTTG-TPGHSPLS-GDSFVYCRTYSETSAFSDP 60

Query: 61  TTDDTSFCTEPSPA-----------------IPTRKYKLNAGDKADDQDAMDSETEMMKE 120
             D+ S C+E SP+                 + T++ K NA DK DDQD++D E +MMKE
Sbjct: 61  V-DNHSCCSEASPSHWPPTRSGAQNQAVLSRLGTKQRKSNADDKLDDQDSLDLELDMMKE 120

Query: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACL 180
           RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  EKKA+WKREM CL
Sbjct: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLNPEKKALWKREMDCL 180

Query: 181 LSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEF 240
           LSVCDYI+EF ++SQ L +GT +E++ SRQRSDIY+NLPAL KLD ML++ILDSF D EF
Sbjct: 181 LSVCDYIVEFSSKSQNLCNGTAVEIMESRQRSDIYINLPALRKLDAMLIEILDSFQDREF 240

Query: 241 WYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFA 300
           WYAE+GSMSSNS    +++ SFRR+  + +EKWW+PVPC+P  GLSEKARK LR+ R+ A
Sbjct: 241 WYAEQGSMSSNS----TRTGSFRRVVQRNDEKWWVPVPCVPPTGLSEKARKCLRHKRDCA 300

Query: 301 NQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLN 360
           NQIHKAAMAINS +LAEM+IP+SY+ +LPKSG+ SIGD +YR+MY+ +KFSPD+LLDCLN
Sbjct: 301 NQIHKAAMAINSGVLAEMEIPDSYMASLPKSGRASIGDPIYRFMYTTEKFSPDYLLDCLN 360

Query: 361 IGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESL 420
           I SEH+ALELADR+EASMYTWRRKAC+SHSKSSW +VKDLM++ D+ DKN ILAERAESL
Sbjct: 361 IASEHEALELADRVEASMYTWRRKACMSHSKSSWGMVKDLMSDVDRSDKNHILAERAESL 420

Query: 421 LLSLKQRFPELSQTTLDTSKIQYNR 429
           L +LKQR+PEL+QT+LDT KIQYNR
Sbjct: 421 LFALKQRYPELAQTSLDTCKIQYNR 438

BLAST of CmaCh08G009070 vs. TrEMBL
Match: W9RSL4_9ROSA (Rop guanine nucleotide exchange factor 2 OS=Morus notabilis GN=L484_017276 PE=4 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 2.6e-158
Identity = 298/449 (66.37%), Postives = 359/449 (79.96%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+  S +D+ + LG+ PS SS+D ND      T G S  S   SF+YCRT+S+ SAFS+ 
Sbjct: 1   MDNLSNTDESYDLGYQPSPSSLDQND-----RTPGYSTFS-GDSFAYCRTNSETSAFSE- 60

Query: 61  TTDDTSFCTEPSP----------------AIPTR---KYKLNAGD--KADDQDAMDSETE 120
           T DD S+ +EPSP                A+ TR   K   N  D  K DDQ+A+DSE E
Sbjct: 61  TIDDQSYSSEPSPSFWPGLKSGGVHSSNQAVLTRLGMKQHKNVADHDKLDDQEALDSELE 120

Query: 121 MMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKRE 180
           +MKERFAKLLLGEDMSGSGKGVC AVTISN+ITNLYATVFGQNL+LEPL  EK+AMWKRE
Sbjct: 121 LMKERFAKLLLGEDMSGSGKGVCAAVTISNSITNLYATVFGQNLRLEPLKLEKRAMWKRE 180

Query: 181 MACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFH 240
           M CLLSVCDYI+EF   SQ L DGT++E+++SR R+DIY+NLPAL+KLDMML++ILDSF 
Sbjct: 181 MDCLLSVCDYIVEFIPTSQNLQDGTSLEIMSSRPRADIYINLPALKKLDMMLIEILDSFQ 240

Query: 241 DTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNN 300
           DTEFWYAE+GSMSSNS  +   S SFRR+  +K+EKWWLP+PC+P  GLSEK+RK LR+ 
Sbjct: 241 DTEFWYAEQGSMSSNSTRS-GPSGSFRRIIQRKDEKWWLPIPCVPPEGLSEKSRKHLRHK 300

Query: 301 REFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLL 360
           R+ ANQIHKAAMAINS+ILAEM+IP+SY+ TLPKSGK  +GD++YRYM SA+KFSPDHLL
Sbjct: 301 RDCANQIHKAAMAINSSILAEMEIPDSYMATLPKSGKACLGDSIYRYMCSAEKFSPDHLL 360

Query: 361 DCLNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAER 420
           D L+I SEH+ALELADR+EASMYTWRRKAC+SHSKSSW++VKDLM++ D+ DKN +LAER
Sbjct: 361 DSLSIASEHEALELADRVEASMYTWRRKACMSHSKSSWDMVKDLMSDTDRSDKNHVLAER 420

Query: 421 AESLLLSLKQRFPELSQTTLDTSKIQYNR 429
           AESLL SLKQ++PELSQT+LDT KIQYNR
Sbjct: 421 AESLLFSLKQKYPELSQTSLDTCKIQYNR 441

BLAST of CmaCh08G009070 vs. TrEMBL
Match: A0A061E106_THECC (RHO guanyl-nucleotide exchange factor 3 isoform 3 (Fragment) OS=Theobroma cacao GN=TCM_005331 PE=4 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.0e-155
Identity = 290/426 (68.08%), Postives = 346/426 (81.22%), Query Frame = 1

Query: 20  SSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDPTTDDTSFCTEPSPA----- 79
           SS+D ND ST   T G SP S   SF YCRT S+ SAFSDP  D+ S C+E SP+     
Sbjct: 2   SSIDQNDQSTTG-TPGHSPLS-GDSFVYCRTYSETSAFSDPV-DNHSCCSEASPSHWPPT 61

Query: 80  ------------IPTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKGVC 139
                       + T++ K NA DK DDQD++D E +MMKERFAKLLLGEDMSGSGKGVC
Sbjct: 62  RSGAQNQAVLSRLGTKQRKSNADDKLDDQDSLDLELDMMKERFAKLLLGEDMSGSGKGVC 121

Query: 140 TAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDD 199
           TAVTISNAITNLYATVFGQNL+LEPL  EKKA+WKREM CLLSVCDYI+EF ++SQ L +
Sbjct: 122 TAVTISNAITNLYATVFGQNLRLEPLNPEKKALWKREMDCLLSVCDYIVEFSSKSQNLCN 181

Query: 200 GTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKS 259
           GT +E++ SRQRSDIY+NLPAL KLD ML++ILDSF D EFWYAE+GSMSSNS    +++
Sbjct: 182 GTAVEIMESRQRSDIYINLPALRKLDAMLIEILDSFQDREFWYAEQGSMSSNS----TRT 241

Query: 260 ASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMD 319
            SFRR+  + +EKWW+PVPC+P  GLSEKARK LR+ R+ ANQIHKAAMAINS +LAEM+
Sbjct: 242 GSFRRVVQRNDEKWWVPVPCVPPTGLSEKARKCLRHKRDCANQIHKAAMAINSGVLAEME 301

Query: 320 IPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMY 379
           IP+SY+ +LPKSG+ SIGD +YR+MY+ +KFSPD+LLDCLNI SEH+ALELADR+EASMY
Sbjct: 302 IPDSYMASLPKSGRASIGDPIYRFMYTTEKFSPDYLLDCLNIASEHEALELADRVEASMY 361

Query: 380 TWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTS 429
           TWRRKAC+SHSKSSW +VKDLM++ D+ DKN ILAERAESLL +LKQR+PEL+QT+LDT 
Sbjct: 362 TWRRKACMSHSKSSWGMVKDLMSDVDRSDKNHILAERAESLLFALKQRYPELAQTSLDTC 420

BLAST of CmaCh08G009070 vs. TAIR10
Match: AT4G00460.2 (AT4G00460.2 RHO guanyl-nucleotide exchange factor 3)

HSP 1 Score: 491.5 bits (1264), Expect = 5.3e-139
Identity = 264/424 (62.26%), Postives = 325/424 (76.65%), Query Frame = 1

Query: 18  SSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDA-SAFSDPTTDDTSFCTEPSPA-- 77
           S  S+D ND S V ET   S  S+  SF Y RT S+  S FSD   +  SFC+E SP   
Sbjct: 15  SPRSIDQNDQSAV-ETPVYSTMSID-SFVYPRTCSETTSGFSDQIDETNSFCSEASPCNW 74

Query: 78  ---IPTRKYKLNAGDKADD------QDAMDSETEMMKERFAKLLLGEDMSGSGKGVCTAV 137
                ++  K  +G +         Q+  + E E MKERFAKLLLGEDMSGSGKGVCTAV
Sbjct: 75  PVLTESKSSKCLSGLEMQSNECLVVQEISEPELETMKERFAKLLLGEDMSGSGKGVCTAV 134

Query: 138 TISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDDGTT 197
           TISNAITNLYATVFGQNL+LEPL  EK+A+WKREM CLLSVCDYI+EF    Q L +G T
Sbjct: 135 TISNAITNLYATVFGQNLRLEPLETEKRALWKREMNCLLSVCDYIVEFIPRCQNLSNGAT 194

Query: 198 MEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKSASF 257
           +EV+ SR R+DIY+NLPAL KLD ML++ LDSF +TEFWYAE+GS+S  S   RS + SF
Sbjct: 195 VEVMESRPRADIYINLPALRKLDSMLMEALDSFQNTEFWYAEEGSLSMKSA--RSSTGSF 254

Query: 258 RRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMDIP 317
           R++ +Q KEEKWWLPVP +PS GLS+KARKQL+N RE  NQIHKAAMAINS+IL+EM+IP
Sbjct: 255 RKVIVQRKEEKWWLPVPLVPSEGLSDKARKQLKNKRESTNQIHKAAMAINSSILSEMEIP 314

Query: 318 ESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMYTW 377
           +SY+ TLPK GK+S+GD++YRYM  + +F P+ LLDCLNI SEH+A++LADR+EASMYTW
Sbjct: 315 DSYMTTLPKCGKSSVGDSIYRYMSGSGRFFPEQLLDCLNISSEHEAVQLADRVEASMYTW 374

Query: 378 RRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTSKI 429
           RRK+CLS+SK+SW +VKDLM+  ++ DKN ++AERAE+LL  LKQR+PELSQT+LD  KI
Sbjct: 375 RRKSCLSNSKNSWNMVKDLMSTTERTDKNYVMAERAETLLFCLKQRYPELSQTSLDICKI 434

BLAST of CmaCh08G009070 vs. TAIR10
Match: AT1G01700.1 (AT1G01700.1 RHO guanyl-nucleotide exchange factor 2)

HSP 1 Score: 480.7 bits (1236), Expect = 9.3e-136
Identity = 267/450 (59.33%), Postives = 325/450 (72.22%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASA--FS 60
           ME     ++   +G+  S   +D ND S  SET   S  S   SF+Y RT S+ S   FS
Sbjct: 1   MENLPNHEENDDVGYHQSPGPIDPNDHSA-SETPVYSTMSTD-SFAYHRTCSETSGGGFS 60

Query: 61  DPTTDDTSFCTEPSPA---------------IPT----RKYKLNAGDKADDQDAMDSETE 120
           D   + +SFCTE SP+                PT    +  ++   +    Q+  + E E
Sbjct: 61  DQIDETSSFCTEASPSDWPVLTESNNSASSNFPTVFDLKHNQIETDEHLAVQEISEPELE 120

Query: 121 MMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKRE 180
            MKERF+KLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  E+K  WKRE
Sbjct: 121 TMKERFSKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLEIEQKTTWKRE 180

Query: 181 MACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFH 240
           M CLLSVCDYI EF  +SQ L +G T+EV+ SR R+DIY+NLPAL KLD ML++ LDSF 
Sbjct: 181 MNCLLSVCDYIFEFIPKSQNLSNGATVEVMESRPRADIYINLPALRKLDSMLMEALDSFQ 240

Query: 241 DTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRN 300
            TEFWYAE+GS+S    S RS + SFR++ +Q KEEKWWLP+P +P  GLSEKARKQL++
Sbjct: 241 KTEFWYAEEGSLS--MKSTRSATGSFRKVIVQRKEEKWWLPIPLVPLQGLSEKARKQLKS 300

Query: 301 NREFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHL 360
            RE  NQIHKAAMAINS+IL EMDIP+SY+ TLPKSGK S GD +YR+M S+ +FSP+ L
Sbjct: 301 KRESTNQIHKAAMAINSSILGEMDIPDSYMATLPKSGKASTGDAIYRHMTSSGRFSPEKL 360

Query: 361 LDCLNIGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAE 420
           LD L I SEH+AL+LADR+EASMYTWRRKACL++SKSSW +VKDLM+  ++ DKN +LAE
Sbjct: 361 LDRLKIVSEHEALQLADRVEASMYTWRRKACLNNSKSSWNMVKDLMSITERSDKNYVLAE 420

Query: 421 RAESLLLSLKQRFPELSQTTLDTSKIQYNR 429
           RAESLL  LKQR+PELSQT+LD  KI  N+
Sbjct: 421 RAESLLFCLKQRYPELSQTSLDICKIHCNK 446

BLAST of CmaCh08G009070 vs. TAIR10
Match: AT2G45890.1 (AT2G45890.1 RHO guanyl-nucleotide exchange factor 4)

HSP 1 Score: 422.9 bits (1086), Expect = 2.3e-118
Identity = 233/431 (54.06%), Postives = 302/431 (70.07%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           ME  S SDQ         +SS+      T S+ SG S      SF    ++++A   S  
Sbjct: 1   MESSSNSDQNEGT----PTSSVSSPYRRTYSDISGLSHRFDVQSFYNRPSNTNAVVLSGH 60

Query: 61  TTDDTSFCTEPSPAIPTRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKG 120
             D +    EP   +    +     + +D   A D+E EMM+ERFAKLLLGEDMSGSGKG
Sbjct: 61  EEDVSEDAEEPKDDVVNDVHGDGDEEDSDIDSAEDAELEMMRERFAKLLLGEDMSGSGKG 120

Query: 121 VCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTL 180
           VCTAVT+SN+ITNLYATVFGQ+L+L+PL  EKK +WKREM C +S+CDYI+E    S   
Sbjct: 121 VCTAVTVSNSITNLYATVFGQSLRLQPLSTEKKDLWKREMNCFMSICDYIVEVIPRSL-- 180

Query: 181 DDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRS 240
             GT +E+  ++ RSDI ++LPAL KLD ML++ILDSF + EFWY E+GS S NS     
Sbjct: 181 --GTNVEITETKLRSDILMSLPALRKLDNMLMEILDSFTENEFWYVERGSSSMNSGGGGR 240

Query: 241 KSASFRRLSLQ-KEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILA 300
            S +FR++ +Q K+EKWWLPVPC+P+ GLSE+ RK LR+ R+ A+QIHKAA+AIN + L 
Sbjct: 241 DSGTFRKVVVQRKDEKWWLPVPCVPAEGLSEEERKHLRHKRDCASQIHKAALAINDSTLN 300

Query: 301 EMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEA 360
           +MDIP+SY+ TLPKSGK S+GD +Y+ + +A+KF PD LLD L I SEH+ALELAD++EA
Sbjct: 301 DMDIPDSYLTTLPKSGKASVGDVIYKQLCTAEKFYPDQLLDILKITSEHEALELADKVEA 360

Query: 361 SMYTWRRK-ACLSHSKSSWELVKDLMAEGDKG-DKNTILAERAESLLLSLKQRFPELSQT 420
           S+ TWRRK   L+HSKSSW+++KD+  + D+G DKN ILA RA SLL  LKQR+PELSQT
Sbjct: 361 SLVTWRRKTGGLTHSKSSWDMMKDISGDADRGNDKNHILAARARSLLFCLKQRYPELSQT 420

Query: 421 TLDTSKIQYNR 429
           +LD  KIQ+NR
Sbjct: 421 SLDICKIQFNR 423

BLAST of CmaCh08G009070 vs. TAIR10
Match: AT5G02010.1 (AT5G02010.1 RHO guanyl-nucleotide exchange factor 7)

HSP 1 Score: 387.1 bits (993), Expect = 1.4e-107
Identity = 208/388 (53.61%), Postives = 280/388 (72.16%), Query Frame = 1

Query: 53  DASAFSDPTT-----DDTSFCTEPSPAIPTRKY----KLNAGDKADDQDAMDSETEMMKE 112
           ++S  S  TT     + +  CTE   A P         ++   K    D+  SE EMMKE
Sbjct: 17  ESSCCSSETTRQEEEEQSPSCTEDFTASPVSSRWSVKNIDGEKKKIRSDSRVSEVEMMKE 76

Query: 113 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACL 172
           RF+KLLLGEDMSGSG GVCTA+ ISNAITNL AT+FGQ  +LEPLP EKK MW+REM  L
Sbjct: 77  RFSKLLLGEDMSGSGNGVCTALAISNAITNLCATLFGQLWRLEPLPTEKKEMWRREMEWL 136

Query: 173 LSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEF 232
           L V D+I+E     QT  DGT +E++  R RSD+Y+NLPAL KLD MLL+ILDSF +TEF
Sbjct: 137 LCVSDHIVEMTPTWQTFPDGTKLEIMTCRPRSDLYVNLPALRKLDNMLLEILDSFEETEF 196

Query: 233 WYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFA 292
           WY ++G M+  S ++   S+SFR+   ++E+KWWLPVP +   GL E +RKQL++ R+  
Sbjct: 197 WYVDQGIMAHESAAD--GSSSFRKSFQRQEDKWWLPVPRVSPGGLQENSRKQLQHKRDCT 256

Query: 293 NQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLN 352
           NQI KAAMAINS  LA+M+IPESY+++LP+ G++ +GD +YRY+ S+D+FSP+ LLDCL+
Sbjct: 257 NQILKAAMAINSITLADMEIPESYLESLPRKGRSCLGDLIYRYI-SSDQFSPECLLDCLD 316

Query: 353 IGSEHDALELADRIEASMYTWRRKA---CLSHSKSSWELVKDLMAEGDKGDKNTILAERA 412
           + SEH A+E+A+R+E+S+Y W ++      +++K+SWE+VK+LM + DK +   ++A+RA
Sbjct: 317 LSSEHQAIEIANRVESSIYLWHKRTNSKPATNTKTSWEMVKELMVDADKLE---LMADRA 376

Query: 413 ESLLLSLKQRFPELSQTTLDTSKIQYNR 429
           ESLLLSLKQRFP L QT LD SKIQYN+
Sbjct: 377 ESLLLSLKQRFPGLPQTALDMSKIQYNK 398

BLAST of CmaCh08G009070 vs. TAIR10
Match: AT5G05940.1 (AT5G05940.1 ROP guanine nucleotide exchange factor 5)

HSP 1 Score: 372.5 bits (955), Expect = 3.6e-103
Identity = 211/421 (50.12%), Postives = 283/421 (67.22%), Query Frame = 1

Query: 18  SSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDPTTDDTSFCTEPSPAIPT 77
           S +S + +  +TV+ +S   PPS    +   + S   ++      D              
Sbjct: 36  SGASYESSSTTTVASSSPPPPPSQILGWPIRKASFRKNSKESVNFDHKKLTLHD------ 95

Query: 78  RKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSGSGKGVCTAVTISNAITNLYAT 137
                ++G KA + ++ D   EMMKERFAKLLLGEDMSGSGKGVCTA+ ISNAITNL AT
Sbjct: 96  -----DSGFKAKEMNSAD--VEMMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLCAT 155

Query: 138 VFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDI 197
           +FGQ  +LEPL +EKK MW+REM  +LSV D+I+E    +QT  DG   EV+  R R D+
Sbjct: 156 IFGQLWRLEPLSSEKKEMWRREMEWILSVSDHIVELTPSTQTYPDGNKFEVMTCRPRFDL 215

Query: 198 YLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWW 257
           ++NLPAL KLD MLLDIL SF  TEFWY ++G ++S ++     SASFRR   ++EEKWW
Sbjct: 216 FINLPALRKLDNMLLDILASFKKTEFWYVDQGIVASENDG----SASFRRKIQRQEEKWW 275

Query: 258 LPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINSTILAEMDIPESYIDTLPKSGKT 317
           LPVP L   GL+E+AR +L + RE A QI KAAMAINS  L EMD+P+SY++TLPK+G++
Sbjct: 276 LPVPRLAPNGLTEEARTELNHKRECATQILKAAMAINSLALTEMDVPKSYLETLPKNGRS 335

Query: 318 SIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADRIEASMYTWRRK---------- 377
            +GD +YRY+ ++DKFS + LLDCL++ SEH AL++A+R+EAS+Y WRR+          
Sbjct: 336 CLGDVIYRYV-TSDKFSAESLLDCLDLSSEHIALDIANRVEASIYVWRRRVQTKLGVNNN 395

Query: 378 ACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQTTLDTSKIQYN 429
              +  K +WE+VK+LMA    GDK  +L ER+E+LL  LKQRFP L+QT+LD SKIQ+N
Sbjct: 396 TSSTTPKLTWEMVKELMA---AGDKRGLLVERSETLLRCLKQRFPSLTQTSLDISKIQWN 435

BLAST of CmaCh08G009070 vs. NCBI nr
Match: gi|449441640|ref|XP_004138590.1| (PREDICTED: rop guanine nucleotide exchange factor 3-like isoform X2 [Cucumis sativus])

HSP 1 Score: 745.7 bits (1924), Expect = 4.4e-212
Identity = 385/432 (89.12%), Postives = 402/432 (93.06%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+KFSISD+ FHLGF PSSSSMDLND ST+S+TSGQSP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPAS-GYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDTSFCTEPSPAIP----TRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSG 120
           TTDD SFCTEPSPAI     TRKYK   GDKADDQDAMDSE EMMKERFAKLLLGEDMSG
Sbjct: 61  TTDDGSFCTEPSPAILGRQCTRKYKHAVGDKADDQDAMDSEMEMMKERFAKLLLGEDMSG 120

Query: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTE 180
           SGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM+CLLSVCDYI+EFFT 
Sbjct: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTL 180

Query: 181 SQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSN 240
           SQTL+DGT++EVLNSRQRSDI LNLPAL+KLDMMLLDILDSF DTEFWYAEKGSMSSNSN
Sbjct: 181 SQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSMSSNSN 240

Query: 241 SNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINST 300
             RS+S SFRRLSLQKEEKWWLPVPC+PSCGLSE ARK LRN RE ANQIHKAAMAINST
Sbjct: 241 --RSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINST 300

Query: 301 ILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADR 360
           ILAEMDIPES+IDTLPKSGK SIGDT+YRYMYSADKFSP+HLLDCLNIGSEHDALELADR
Sbjct: 301 ILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADR 360

Query: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQ 420
           IEASMYTWRRKACLSHSKSSWELVKDLMAE +K DKNTILAERAE+LLLSLKQRFPELSQ
Sbjct: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQ 420

Query: 421 TTLDTSKIQYNR 429
           TTLDTSKIQYNR
Sbjct: 421 TTLDTSKIQYNR 429

BLAST of CmaCh08G009070 vs. NCBI nr
Match: gi|659116739|ref|XP_008458234.1| (PREDICTED: rop guanine nucleotide exchange factor 3 isoform X2 [Cucumis melo])

HSP 1 Score: 740.7 bits (1911), Expect = 1.4e-210
Identity = 383/432 (88.66%), Postives = 402/432 (93.06%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+KFSISD+ FHLGF PSSSSMDLND ST+S+TSGQSP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPAS-GYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDTSFCTEPSPAIP----TRKYKLNAGDKADDQDAMDSETEMMKERFAKLLLGEDMSG 120
           TTDD+SF TEPSPAI     TRKYK  AGDKADD DAMDSE EMMKERFAKLLLGEDMSG
Sbjct: 61  TTDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSEMEMMKERFAKLLLGEDMSG 120

Query: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYILEFFTE 180
           SGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM+CLLSVCDYI+EFFT 
Sbjct: 121 SGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIVEFFTL 180

Query: 181 SQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSMSSNSN 240
           SQTL+DGT++EVLNSRQRSDI LNLPAL+KLDMMLLDILDSF DTEFWYAEKGS+SSNSN
Sbjct: 181 SQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSVSSNSN 240

Query: 241 SNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAMAINST 300
             RS+S SFRRLSLQKEEKWWLPVPC+PSCGLSE ARK LRN RE ANQIHKAAMAINST
Sbjct: 241 --RSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAMAINST 300

Query: 301 ILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDALELADR 360
           ILAEMDIPES+IDTLPKSGK SIGDT+YRYMYSADKFSP+HLLDCLNIGSEHDALELADR
Sbjct: 301 ILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDALELADR 360

Query: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRFPELSQ 420
           IEASMYTWRRKACLSHSKSSWELVKDLMAE +K DKNTILAERAE+LLLSLKQRFPELSQ
Sbjct: 361 IEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRFPELSQ 420

Query: 421 TTLDTSKIQYNR 429
           TTLDTSKIQYNR
Sbjct: 421 TTLDTSKIQYNR 429

BLAST of CmaCh08G009070 vs. NCBI nr
Match: gi|778709085|ref|XP_011656340.1| (PREDICTED: rop guanine nucleotide exchange factor 3-like isoform X1 [Cucumis sativus])

HSP 1 Score: 739.6 bits (1908), Expect = 3.2e-210
Identity = 385/437 (88.10%), Postives = 402/437 (91.99%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+KFSISD+ FHLGF PSSSSMDLND ST+S+TSGQSP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPAS-GYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDTSFCTEPSPAIP----TRKYKLNAGDKADDQDAMDS-----ETEMMKERFAKLLLG 120
           TTDD SFCTEPSPAI     TRKYK   GDKADDQDAMDS     E EMMKERFAKLLLG
Sbjct: 61  TTDDGSFCTEPSPAILGRQCTRKYKHAVGDKADDQDAMDSVVVITEMEMMKERFAKLLLG 120

Query: 121 EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYIL 180
           EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM+CLLSVCDYI+
Sbjct: 121 EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIV 180

Query: 181 EFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSM 240
           EFFT SQTL+DGT++EVLNSRQRSDI LNLPAL+KLDMMLLDILDSF DTEFWYAEKGSM
Sbjct: 181 EFFTLSQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSM 240

Query: 241 SSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAM 300
           SSNSN  RS+S SFRRLSLQKEEKWWLPVPC+PSCGLSE ARK LRN RE ANQIHKAAM
Sbjct: 241 SSNSN--RSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAM 300

Query: 301 AINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDAL 360
           AINSTILAEMDIPES+IDTLPKSGK SIGDT+YRYMYSADKFSP+HLLDCLNIGSEHDAL
Sbjct: 301 AINSTILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDAL 360

Query: 361 ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRF 420
           ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAE +K DKNTILAERAE+LLLSLKQRF
Sbjct: 361 ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRF 420

Query: 421 PELSQTTLDTSKIQYNR 429
           PELSQTTLDTSKIQYNR
Sbjct: 421 PELSQTTLDTSKIQYNR 434

BLAST of CmaCh08G009070 vs. NCBI nr
Match: gi|659116737|ref|XP_008458233.1| (PREDICTED: rop guanine nucleotide exchange factor 3 isoform X1 [Cucumis melo])

HSP 1 Score: 734.6 bits (1895), Expect = 1.0e-208
Identity = 383/437 (87.64%), Postives = 402/437 (91.99%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+KFSISD+ FHLGF PSSSSMDLND ST+S+TSGQSP S  YSFSYCRTSSDASAFSDP
Sbjct: 1   MDKFSISDENFHLGFRPSSSSMDLNDHSTISDTSGQSPAS-GYSFSYCRTSSDASAFSDP 60

Query: 61  TTDDTSFCTEPSPAIP----TRKYKLNAGDKADDQDAMDS-----ETEMMKERFAKLLLG 120
           TTDD+SF TEPSPAI     TRKYK  AGDKADD DAMDS     E EMMKERFAKLLLG
Sbjct: 61  TTDDSSFFTEPSPAILGRQCTRKYKHAAGDKADDHDAMDSVVVITEMEMMKERFAKLLLG 120

Query: 121 EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACLLSVCDYIL 180
           EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLP EKKAMWKREM+CLLSVCDYI+
Sbjct: 121 EDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPPEKKAMWKREMSCLLSVCDYIV 180

Query: 181 EFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEFWYAEKGSM 240
           EFFT SQTL+DGT++EVLNSRQRSDI LNLPAL+KLDMMLLDILDSF DTEFWYAEKGS+
Sbjct: 181 EFFTLSQTLEDGTSLEVLNSRQRSDICLNLPALQKLDMMLLDILDSFQDTEFWYAEKGSV 240

Query: 241 SSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFANQIHKAAM 300
           SSNSN  RS+S SFRRLSLQKEEKWWLPVPC+PSCGLSE ARK LRN RE ANQIHKAAM
Sbjct: 241 SSNSN--RSRSGSFRRLSLQKEEKWWLPVPCIPSCGLSENARKHLRNKRECANQIHKAAM 300

Query: 301 AINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLNIGSEHDAL 360
           AINSTILAEMDIPES+IDTLPKSGK SIGDT+YRYMYSADKFSP+HLLDCLNIGSEHDAL
Sbjct: 301 AINSTILAEMDIPESFIDTLPKSGKASIGDTIYRYMYSADKFSPEHLLDCLNIGSEHDAL 360

Query: 361 ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESLLLSLKQRF 420
           ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAE +K DKNTILAERAE+LLLSLKQRF
Sbjct: 361 ELADRIEASMYTWRRKACLSHSKSSWELVKDLMAETEKSDKNTILAERAETLLLSLKQRF 420

Query: 421 PELSQTTLDTSKIQYNR 429
           PELSQTTLDTSKIQYNR
Sbjct: 421 PELSQTTLDTSKIQYNR 434

BLAST of CmaCh08G009070 vs. NCBI nr
Match: gi|590722117|ref|XP_007051807.1| (RHO guanyl-nucleotide exchange factor 2 isoform 4 [Theobroma cacao])

HSP 1 Score: 574.7 bits (1480), Expect = 1.3e-160
Identity = 299/446 (67.04%), Postives = 359/446 (80.49%), Query Frame = 1

Query: 1   MEKFSISDQKFHLGFCPSSSSMDLNDPSTVSETSGQSPPSLAYSFSYCRTSSDASAFSDP 60
           M+  S SD+ + LG+ PS SS+D ND ST   T G SP S   SF YCRT S+ SAFSDP
Sbjct: 1   MDSMSNSDENYDLGYQPSPSSIDQNDQSTTG-TPGHSPLS-GDSFVYCRTYSETSAFSDP 60

Query: 61  TTDDTSFCTEPSPA-----------------IPTRKYKLNAGDKADDQDAMDSETEMMKE 120
             D+ S C+E SP+                 + T++ K NA DK DDQD++D E +MMKE
Sbjct: 61  V-DNHSCCSEASPSHWPPTRSGAQNQAVLSRLGTKQRKSNADDKLDDQDSLDLELDMMKE 120

Query: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLKLEPLPAEKKAMWKREMACL 180
           RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNL+LEPL  EKKA+WKREM CL
Sbjct: 121 RFAKLLLGEDMSGSGKGVCTAVTISNAITNLYATVFGQNLRLEPLNPEKKALWKREMDCL 180

Query: 181 LSVCDYILEFFTESQTLDDGTTMEVLNSRQRSDIYLNLPALEKLDMMLLDILDSFHDTEF 240
           LSVCDYI+EF ++SQ L +GT +E++ SRQRSDIY+NLPAL KLD ML++ILDSF D EF
Sbjct: 181 LSVCDYIVEFSSKSQNLCNGTAVEIMESRQRSDIYINLPALRKLDAMLIEILDSFQDREF 240

Query: 241 WYAEKGSMSSNSNSNRSKSASFRRLSLQKEEKWWLPVPCLPSCGLSEKARKQLRNNREFA 300
           WYAE+GSMSSNS    +++ SFRR+  + +EKWW+PVPC+P  GLSEKARK LR+ R+ A
Sbjct: 241 WYAEQGSMSSNS----TRTGSFRRVVQRNDEKWWVPVPCVPPTGLSEKARKCLRHKRDCA 300

Query: 301 NQIHKAAMAINSTILAEMDIPESYIDTLPKSGKTSIGDTMYRYMYSADKFSPDHLLDCLN 360
           NQIHKAAMAINS +LAEM+IP+SY+ +LPKSG+ SIGD +YR+MY+ +KFSPD+LLDCLN
Sbjct: 301 NQIHKAAMAINSGVLAEMEIPDSYMASLPKSGRASIGDPIYRFMYTTEKFSPDYLLDCLN 360

Query: 361 IGSEHDALELADRIEASMYTWRRKACLSHSKSSWELVKDLMAEGDKGDKNTILAERAESL 420
           I SEH+ALELADR+EASMYTWRRKAC+SHSKSSW +VKDLM++ D+ DKN ILAERAESL
Sbjct: 361 IASEHEALELADRVEASMYTWRRKACMSHSKSSWGMVKDLMSDVDRSDKNHILAERAESL 420

Query: 421 LLSLKQRFPELSQTTLDTSKIQYNRV 430
           L +LKQR+PEL+QT+LDT KIQYNRV
Sbjct: 421 LFALKQRYPELAQTSLDTCKIQYNRV 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ROGF3_ARATH9.4e-13862.26Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana GN=ROPGEF3 PE=2... [more]
ROGF2_ARATH1.7e-13459.33Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana GN=ROPGEF2 PE=1... [more]
ROGF4_ARATH4.1e-11754.06Rop guanine nucleotide exchange factor 4 OS=Arabidopsis thaliana GN=ROPGEF4 PE=1... [more]
ROGF7_ARATH2.5e-10653.61Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana GN=ROPGEF7 PE=1... [more]
ROGF5_ARATH6.3e-10250.12Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana GN=ROPGEF5 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0K849_CUCSA3.1e-21289.12Uncharacterized protein OS=Cucumis sativus GN=Csa_6G004490 PE=4 SV=1[more]
A0A061DT39_THECC9.4e-16167.04RHO guanyl-nucleotide exchange factor 2 isoform 4 OS=Theobroma cacao GN=TCM_0053... [more]
A0A061DU87_THECC3.6e-16066.97RHO guanyl-nucleotide exchange factor 3 isoform 1 OS=Theobroma cacao GN=TCM_0053... [more]
W9RSL4_9ROSA2.6e-15866.37Rop guanine nucleotide exchange factor 2 OS=Morus notabilis GN=L484_017276 PE=4 ... [more]
A0A061E106_THECC2.0e-15568.08RHO guanyl-nucleotide exchange factor 3 isoform 3 (Fragment) OS=Theobroma cacao ... [more]
Match NameE-valueIdentityDescription
AT4G00460.25.3e-13962.26 RHO guanyl-nucleotide exchange factor 3[more]
AT1G01700.19.3e-13659.33 RHO guanyl-nucleotide exchange factor 2[more]
AT2G45890.12.3e-11854.06 RHO guanyl-nucleotide exchange factor 4[more]
AT5G02010.11.4e-10753.61 RHO guanyl-nucleotide exchange factor 7[more]
AT5G05940.13.6e-10350.12 ROP guanine nucleotide exchange factor 5[more]
Match NameE-valueIdentityDescription
gi|449441640|ref|XP_004138590.1|4.4e-21289.12PREDICTED: rop guanine nucleotide exchange factor 3-like isoform X2 [Cucumis sat... [more]
gi|659116739|ref|XP_008458234.1|1.4e-21088.66PREDICTED: rop guanine nucleotide exchange factor 3 isoform X2 [Cucumis melo][more]
gi|778709085|ref|XP_011656340.1|3.2e-21088.10PREDICTED: rop guanine nucleotide exchange factor 3-like isoform X1 [Cucumis sat... [more]
gi|659116737|ref|XP_008458233.1|1.0e-20887.64PREDICTED: rop guanine nucleotide exchange factor 3 isoform X1 [Cucumis melo][more]
gi|590722117|ref|XP_007051807.1|1.3e-16067.04RHO guanyl-nucleotide exchange factor 2 isoform 4 [Theobroma cacao][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR005512PRONE_dom
Vocabulary: Molecular Function
TermDefinition
GO:0005089Rho guanyl-nucleotide exchange factor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043547 positive regulation of GTPase activity
biological_process GO:0043087 regulation of GTPase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0005089 Rho guanyl-nucleotide exchange factor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh08G009070.1CmaCh08G009070.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005512PRONE domainPFAMPF03759PRONEcoord: 96..428
score: 1.7E
IPR005512PRONE domainPROFILEPS51334PRONEcoord: 88..430
score: 97
NoneNo IPR availablePANTHERPTHR33101FAMILY NOT NAMEDcoord: 1..428
score: 5.9E
NoneNo IPR availablePANTHERPTHR33101:SF9ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2-RELATEDcoord: 1..428
score: 5.9E

The following gene(s) are paralogous to this gene:

None