CmaCh08G005710 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh08G005710 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 1.2e-227
Identity = 385/534 (72.10%), Postives = 454/534 (85.02%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 69
           + IFRSKLPDI+IPNHLPLH Y F+N S+  SRPCLINGA   +Y+Y DV+L +R+VA+G
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRKVAAG 68

Query: 70  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 129
           LH  GI+  + +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA++ K+I
Sbjct: 69  LHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKII 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADDVVALPY 189
           VT AC  ++VKD A EN VKI+C+D A EGCLH+SVL+ A+E  +P V+   DDVVALPY
Sbjct: 129 VTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY H  DV+LCVLP FHIYSLNS+LLCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLLCG 248

Query: 250 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 309
           LR GAAILIMQKF+IVS L+L+++YKV+I P VPPI LAIAKSP ++ YD+SSVR + SG
Sbjct: 249 LRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVMSG 308

Query: 310 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
            APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGTVVRNAE
Sbjct: 309 AAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 429
           MKIVDP+TG SLP N +GEICIRGDQIMKGYLND E+T RT+DKEGWL+TGDIG++DD++
Sbjct: 369 MKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDDDD 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+ M DE+AGEVPVAFVV++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNG 488

Query: 490 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGAYN 543
             ITE++V  FIS+QV+FYKR+KRVFFV+AIPK+PSGKILRK+LRAKL  G  N
Sbjct: 489 STITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542

BLAST of CmaCh08G005710 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-226
Identity = 381/540 (70.56%), Postives = 451/540 (83.52%), Query Frame = 1

Query: 4   ELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTA 63
           E  Q+ + IFRSKLPDI+IP HLPLH Y F+N S+  SRPCLINGA   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKA 123
           R+VA GL+ LGI++ + +M LLPNSPEFVF F+GASY GAI T ANP +T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 ANVKLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADD 183
           ++ K+I+T +CF  +VKD A EN VK++C+D A EGCLH+S L+ +DE  +P V    DD
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY H  DV++CVLP FHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSV 303
           SILLCGLR GAAILIMQKF+I   L+L++KYKVSI P VPPI LAIAKSP ++ YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGT 363
           R + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GACGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIG 423
           VVRNAEMKIVDP+TG SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAF 483
           F+D+++ELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+ M DE+AGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGAYN 543
           VV++NG AITE++V  FIS+QV+FYKR+KRVFFV  +PK+PSGKILRK+LRA+L  G  N
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVPN 547

BLAST of CmaCh08G005710 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.5e-225
Identity = 387/540 (71.67%), Postives = 453/540 (83.89%), Query Frame = 1

Query: 7   QTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRV 66
           Q  + IFRSKLPDI+IP +LPLH Y F+N SK  SRPCLINGAT ++++Y DV+L +RRV
Sbjct: 10  QKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRV 69

Query: 67  ASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANV 126
            SGL  LGIK+G+ +M LLPNSPEFVF FLGAS+ G+I T ANPF+T+ E+ KQAKA+N 
Sbjct: 70  GSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKASNA 129

Query: 127 KLIVTMACFYDRVKDLA-ENGVKIVCVDYAVEG-----CLHYSVLSGADESHVPFVDFAA 186
           KLI+T  C+ D+VKD A ENGVKI+ +D           LH+S L+GADE+ +P V+ + 
Sbjct: 130 KLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMPKVEISP 189

Query: 187 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 246
           D VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY H  DV+LCVLP FHIYS
Sbjct: 190 DGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFHIYS 249

Query: 247 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 306
           LNS+LLCGLRAG+ ILIMQKFEIV  L+L++KYKV+I P VPPI LAIAKS  ++ YD+S
Sbjct: 250 LNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNYDLS 309

Query: 307 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 366
           SVR + SG APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K+GAC
Sbjct: 310 SVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 369

Query: 367 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 426
           GTVVRNAEMKIVDPETG+SLP N  GEICIRGDQIMKGYLND E+T RT+DKEGWLHTGD
Sbjct: 370 GTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLHTGD 429

Query: 427 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 486
           IG++DD++ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M DE AGEVPV
Sbjct: 430 IGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGEVPV 489

Query: 487 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 541
           AFVVK+NG  ITE+++ QFIS+QV+FYKR+ RVFFV AIPKAPSGKILRK+LRA+L   A
Sbjct: 490 AFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLAAAA 549

BLAST of CmaCh08G005710 vs. Swiss-Prot
Match: 4CL1_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 2.8e-224
Identity = 376/531 (70.81%), Postives = 451/531 (84.93%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 69
           + IFRSKLPDI+IP HLPLH Y F+N SK+G + CLINGATG+ ++Y  V+L +R+VASG
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 129
           L+ LGI++G+ +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+  KLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKLI 129

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADDVVALPY 189
           +T AC+ D+VKD A E  ++I+C+D A + CLH+S L  ADES +P V   +DDVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY H  DV++C+LP FHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 309
           LRAG  ILIMQKF+IV  L+L++KYKV+I P VPPI LAIAKSP ++KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
            APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEP+ +K+GACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 429
           MKIVDPET ASLP N  GEICIRGDQIMKGYLND EST+ T+D+EGWLHTGDIGF+DD++
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 489
           ELFIVDRLKE+IK+K FQVAPAELEALL+THP +SDAAV+ M DE+AGEVPVAFVV+ NG
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNG 489

Query: 490 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNG 540
              TEE++ QF+S+QVVFYKR+ RVFFV+AIPK+PSGKILRK+LRA++ +G
Sbjct: 490 FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASG 540

BLAST of CmaCh08G005710 vs. Swiss-Prot
Match: 4CL2_PETCR (4-coumarate--CoA ligase 1 OS=Petroselinum crispum GN=4CL2 PE=2 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 2.8e-224
Identity = 377/531 (71.00%), Postives = 451/531 (84.93%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 69
           + IFRSKLPDI+IP HLPLH Y F+N SK+G + CLINGATG+ ++Y  V+L +R+VASG
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 129
           L+ LGI++G+ +M LLPNSPE+ F FLGASYRGAI T ANPF+T+ E+ KQ KA+  KLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLI 129

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADDVVALPY 189
           +T AC+ D+VKD A E  ++I+C+D A + CLH+S L  ADES +P V   +DDVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY H  DV++C+LP FHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 309
           LRAG  ILIMQKF+IV  L+L++KYKV+I P VPPI LAIAKSP ++KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
            APLGKELEDAVR KFP A LGQGYGMTEAGPVL M LAFAKEP+ +K+GACGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 429
           MKIVDPET ASLP N  GEICIRGDQIMKGYLND EST+ T+D+EGWLHTGDIGF+DD++
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 489
           ELFIVDRLKE+IK+K FQVAPAELEALL+THP +SDAAV+ M DE+AGEVPVAFVV+ NG
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNG 489

Query: 490 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNG 540
              TEE++ QF+S+QVVFYKR+ RVFFV+AIPK+PSGKILRK+LRAK+ +G
Sbjct: 490 FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540

BLAST of CmaCh08G005710 vs. TrEMBL
Match: A0A0A0K8H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 3.4e-285
Identity = 492/542 (90.77%), Postives = 521/542 (96.13%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E N+TNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGV+IVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYYH  DVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR GAAI+IMQKF+IV+LLQL+EK+++SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AILGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD+ ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFV+KANGGAI+EE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. TrEMBL
Match: A0A0A0K658_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 1.3e-284
Identity = 492/542 (90.77%), Postives = 520/542 (95.94%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E N+TNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGD Y+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGV++VCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYYH  DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR GAAILIMQKF+IVSLLQL+EK+++SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFVVKANGGAITEE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 1.5e-272
Identity = 474/545 (86.97%), Postives = 503/545 (92.29%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNQTNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDL ENGVKIVCVD++ EGCLH+SVLSGADES +  VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLGENGVKIVCVDFSAEGCLHFSVLSGADESLMSRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY G DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLRAGAAI+IMQKF+IVSL QL+ KYK+SI+PIVPPIFLAIAKS + EKYDVS
Sbjct: 241 LNSILLCGLRAGAAIMIMQKFDIVSLFQLIGKYKISIVPIVPPIFLAIAKSAEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFPAAIL QGYGMTEAGPVLTMSLAFAKEPF VK  AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKFEAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETG SLP NSAGEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGVSLPENSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGF+DD  ELFI+DRLKELIKFK FQVAPAELEALLITHPKL DAAVIGMPD EAGEVPV
Sbjct: 421 IGFIDDNNELFIIDRLKELIKFKTFQVAPAELEALLITHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGA---ITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLG 540
           AFVV+   GA    TEE+V +FI++QV+FYKRLKRVFFVNAIPKAPSGKILRKE+RA L 
Sbjct: 481 AFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKRVFFVNAIPKAPSGKILRKEIRANLA 540

Query: 541 NGAYN 543
           +GAYN
Sbjct: 541 SGAYN 545

BLAST of CmaCh08G005710 vs. TrEMBL
Match: Q5S017_BETPL (4-coumarate:CoA ligase OS=Betula platyphylla PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.5e-235
Identity = 398/534 (74.53%), Postives = 464/534 (86.89%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 69
           EFIFRSKLPDI+IP HLP+H Y F+N SK+GSRPCLING TG VY+Y+DV+LTAR+VASG
Sbjct: 9   EFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVELTARKVASG 68

Query: 70  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 129
           L  LGI+KG+VVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQAK+AN K++
Sbjct: 69  LSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQAKSANAKIV 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADDVVALPY 189
           VT AC+YDRVKD   ENGVKI+C+D   E CLH+S L+ ADE+ V  VD + DDVVALPY
Sbjct: 129 VTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDISPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIYSLNS+ LCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIYSLNSVFLCG 248

Query: 250 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 309
           LRAGA+ILI+ KFEIVSLLQL++K+KV++MPIVPPI LAI K PDL+KYD+SSV++LKSG
Sbjct: 249 LRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDLSSVKMLKSG 308

Query: 310 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
           GAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL M LAFAKEP  VK+GACGTVVRNAE
Sbjct: 309 GAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 429
           MKIVDPETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTGDIG +DD +
Sbjct: 369 MKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTGDIGLIDDND 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D+ AGEVPVAFV ++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVPVAFVARSNG 488

Query: 490 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGAYN 543
             +TE+++ QF+S+QVVFYKR+ RVFF++ +PK+PSGKILRKELRAKL  G  N
Sbjct: 489 SQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAGFSN 542

BLAST of CmaCh08G005710 vs. TrEMBL
Match: A0A089YQ16_BETPN (4-coumarate:CoA ligase 1 OS=Betula pendula PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 2.5e-235
Identity = 398/534 (74.53%), Postives = 464/534 (86.89%), Query Frame = 1

Query: 10  EFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 69
           EFIFRSKLPDI+IP HLP+H Y F+N SK+GSRPCLING TG VY+Y+DV+LTAR+VASG
Sbjct: 9   EFIFRSKLPDIYIPKHLPIHSYCFENISKVGSRPCLINGLTGKVYTYYDVELTARKVASG 68

Query: 70  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 129
           L  LGI+KG+VVM LLPNSPEF F FLGASY GA+ TAANPF TA E++KQAK+AN K++
Sbjct: 69  LSKLGIQKGDVVMLLLPNSPEFAFVFLGASYLGAMTTAANPFCTAGEVSKQAKSANAKIV 128

Query: 130 VTMACFYDRVKDLA-ENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAADDVVALPY 189
           VT AC+YDRVKD   ENGVKI+C+D   E CLH+S L+ ADE+ V  VD + DDVVALPY
Sbjct: 129 VTQACYYDRVKDYTNENGVKIICIDSPPEDCLHFSELTKADENDVAEVDISPDDVVALPY 188

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLYYH  DVILCVLP FHIYSLNS+ LCG
Sbjct: 189 SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYHSEDVILCVLPLFHIYSLNSVFLCG 248

Query: 250 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 309
           LRAGA+ILI+ KFEIVSLLQL++K+KV++MPIVPPI LAI K PDL+KYD+SSV++LKSG
Sbjct: 249 LRAGASILILPKFEIVSLLQLIQKHKVTVMPIVPPIVLAITKFPDLDKYDLSSVKMLKSG 308

Query: 310 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 369
           GAPLGKE+E+ V+ KFP A+ GQGYGMTEAGPVL M LAFAKEP  VK+GACGTVVRNAE
Sbjct: 309 GAPLGKEIEETVKAKFPNALFGQGYGMTEAGPVLAMCLAFAKEPMEVKSGACGTVVRNAE 368

Query: 370 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 429
           MKIVDPETGASLP N  GEICIRGDQIMKGY+ND E+T  T+DKEGWLHTGDIG +DD +
Sbjct: 369 MKIVDPETGASLPRNQPGEICIRGDQIMKGYINDPEATASTIDKEGWLHTGDIGLIDDND 428

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 489
           ELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+ M D+ AGEVPVAFV ++NG
Sbjct: 429 ELFIVDRLKELIKYKGFQVAPAELEALLLTHPNISDAAVVPMKDDLAGEVPVAFVARSNG 488

Query: 490 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGAYN 543
             +TE+++ QF+S+QVVFYKR+ RVFF++ +PK+PSGKILRKELRAKL  G  N
Sbjct: 489 SQVTEDEIKQFVSKQVVFYKRISRVFFIDVVPKSPSGKILRKELRAKLAAGFSN 542

BLAST of CmaCh08G005710 vs. TAIR10
Match: AT1G51680.1 (AT1G51680.1 4-coumarate:CoA ligase 1)

HSP 1 Score: 739.6 bits (1908), Expect = 1.4e-213
Identity = 369/543 (67.96%), Postives = 442/543 (81.40%), Query Frame = 1

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARR 65
           N  ++ IFRSKLPDI+IPNHL LH Y+FQN S+  ++PCLING TG VY+Y DV + +R+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAAN 125
           +A+  H LG+ + +VVM LLPN PEFV +FL AS+RGA  TAANPF+T  EIAKQAKA+N
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 VKLIVTMACFYDRVKDLA-ENGVKIVCVDY-----AVEGCLHYSVL--SGADESHV-PFV 185
            KLI+T A + D++K L  ++GV IVC+D        EGCL ++ L  S  + S V   V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 DFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFF 245
           + + DDVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+H  DVILCVLP F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL+L+++ KV++ P+VPPI LAIAKS + EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+RV+KSG APLGKELEDAV  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWL 425
           +GACGTVVRNAEMKIVDP+TG SL  N  GEICIRG QIMKGYLN+  +T  T+DK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAG 485
           HTGDIG +DD++ELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+ M +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 540
           EVPVAFVVK+    ++E+DV QF+S+QVVFYKR+ +VFF  +IPKAPSGKILRK+LRAKL
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of CmaCh08G005710 vs. TAIR10
Match: AT3G21240.1 (AT3G21240.1 4-coumarate:CoA ligase 2)

HSP 1 Score: 739.2 bits (1907), Expect = 1.8e-213
Identity = 359/539 (66.60%), Postives = 449/539 (83.30%), Query Frame = 1

Query: 8   TNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVA 67
           +N+ IFRS+LPDI+IPNHLPLH Y+F+N S+  ++PCLING TG+VY+Y DV +T+R++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVK 127
           +GLHNLG+K+ +VVM LLPNSPE V TFL AS+ GAI T+ANPF+T  EI+KQAKA+  K
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDRVKDLAENGVKIVCVDYAV--EGCLHYSVLSGADESHVPFVD--FAADDV 187
           LIVT + + D++K+L  +GV IV  D     E CL +S L+ ++E  V  +    + +DV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY++  DVILCVLP FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVR 307
           I+LC LR GA ILIM KFEI  LL+ +++ KV++  +VPPI LAIAKSP+ EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 VLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTV 367
           ++KSG APLGKELEDA+  KFP A LGQGYGMTEAGPVL MSL FAKEPFPVK+GACGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGF 427
           VRNAEMKI+DP+TG SLP N  GEICIRG+QIMKGYLND  +T  T+DK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 VDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFV 487
           +DD++ELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+ M +E+AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGAYN 543
           V++    I+E+++ QF+S+QVVFYKR+ +VFF ++IPKAPSGKILRK+LRA+L NG  N
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLMN 556

BLAST of CmaCh08G005710 vs. TAIR10
Match: AT3G21230.1 (AT3G21230.1 4-coumarate:CoA ligase 5)

HSP 1 Score: 673.3 bits (1736), Expect = 1.2e-193
Identity = 329/546 (60.26%), Postives = 424/546 (77.66%), Query Frame = 1

Query: 4   ELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLG----SRPCLINGATGDVYSYHDV 63
           E   +++FIFRSKLPDI IPNHLPL  YVFQ  S  G    S  C+I+GATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAK 123
           Q   RR+A+G+H LGI+ G+VVM LLPNSPEF  +FL  +Y GA+ T ANPFYT  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAANVKLIVTMACFYDRVKDLAENGVKIVCVD---------YAVEGCLHYSVLSGADE 183
           QAKA+  K+I+T  C  D++ +L  +GV IVC+D          + +GC+ ++ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHVPFVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVIL 243
           + +     + +D VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +   DVIL
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAK 303
           C LP FHIY+L++++L  +R GAA+LI+ +FE+  +++L+++YKV+++P+ PP+ LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPDLEKYDVSSVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAK 363
           SP+ E+YD+SSVR++ SG A L KELEDAVR KFP AI GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTV 423
            PF  K+GACGTV+RNAEMK+VD ETG SLP N +GEIC+RG Q+MKGYLND E+T RT+
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGM 483
           DK+GWLHTGDIGFVDD++E+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+ M
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 PDEEAGEVPVAFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRK 537
            DE A EVPVAFV ++ G  +TE+DV  ++++QVV YKR+K VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of CmaCh08G005710 vs. TAIR10
Match: AT1G65060.1 (AT1G65060.1 4-coumarate:CoA ligase 3)

HSP 1 Score: 670.6 bits (1729), Expect = 8.0e-193
Identity = 333/528 (63.07%), Postives = 416/528 (78.79%), Query Frame = 1

Query: 12  IFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASGLH 71
           IFRSKLPDI IPNHLPLH Y F+  S +  +PCLI G+TG  Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  NLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLIVT 131
            LGI+KG+V+M LL NS EFVF+F+GAS  GA+ T ANPFYT+ E+ KQ K++  KLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPF---VDFAADDVVALPY 191
            + + D++K+L EN   I   +   E CL +S L   DE++ PF   VD   DD  ALP+
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETN-PFQETVDIGGDDAAALPF 213

Query: 192 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYSLNSILLCG 251
           SSGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY    DVILCVLP FHIYSLNS+LL  
Sbjct: 214 SSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNS 273

Query: 252 LRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVSSVRVLKSG 311
           LR+GA +L+M KFEI +LL L+++++V+I  +VPP+ +A+AK+P +  YD+SSVR + SG
Sbjct: 274 LRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSG 333

Query: 312 GAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGACGTVVRNAE 371
            APLGKEL+D++R + P AILGQGYGMTEAGPVL+MSL FAKEP P K+G+CGTVVRNAE
Sbjct: 334 AAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAE 393

Query: 372 MKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGDIGFVDDEE 431
           +K+V  ET  SL  N  GEICIRG QIMK YLND E+T  T+D+EGWLHTGDIG+VD+++
Sbjct: 394 LKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDD 453

Query: 432 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPVAFVVKANG 491
           E+FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+   DE AGEVPVAFVV++NG
Sbjct: 454 EIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNG 513

Query: 492 GAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL 537
             ITEEDV +++++QVVFYKRL +VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 NDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of CmaCh08G005710 vs. TAIR10
Match: AT4G05160.1 (AT4G05160.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 408.3 bits (1048), Expect = 7.4e-114
Identity = 223/541 (41.22%), Postives = 331/541 (61.18%), Query Frame = 1

Query: 12  IFRSKLPDIHIPN--HLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQLTARRVASG 71
           I+RS  P + +P   +  L  ++F+N S   S+  + +  TGD  ++  ++    R+A G
Sbjct: 11  IYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQLKSAVARLAHG 70

Query: 72  LHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQAKAANVKLI 131
            H LGI+K +VV+   PNS +F   FL  +  G + T ANP YT  E++KQ K +N K+I
Sbjct: 71  FHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKII 130

Query: 132 VTMACFYDRVK------------DLAE----NGVKIVCVDYAVEGCLHYSVLSGADESHV 191
           +++   +D++K            D  E    +  KI+  D  +E             S  
Sbjct: 131 ISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVME--------LSEPVSEY 190

Query: 192 PFVDFAADDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVL 251
           PFV+    D  AL YSSGTTG  KGV LTH   I +       Q+    YHG  V LC L
Sbjct: 191 PFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHG--VFLCFL 250

Query: 252 PFFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPD 311
           P FH++ L  I    L+ G A++ M +FE+  +L+ +EK++V+ + +VPP+FLA++K   
Sbjct: 251 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 310

Query: 312 LEKYDVSSVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPF 371
           ++K+D+SS++ + SG APLGK+L +      P  +L QGYGMTE     T  +   ++P 
Sbjct: 311 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTE-----TCGIVSVEDPR 370

Query: 372 PVK--AGACGTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVD 431
             K  +G+ G +    E +IV  ETG S P N  GEI +RG  +MKGYLN+ ++TK T+D
Sbjct: 371 LGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETID 430

Query: 432 KEGWLHTGDIGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMP 491
           K+ W+HTGD+G+ +++  L++VDR+KELIK+K FQVAPAELE LL++HP + DA VI  P
Sbjct: 431 KKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFP 490

Query: 492 DEEAGEVPVAFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKE 533
           DEEAGEVP+AFVV++   +ITE+D+ +FI++QV  YKRL+RV F++ +PK+ +GKILR+E
Sbjct: 491 DEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLRRVSFISLVPKSAAGKILRRE 536

BLAST of CmaCh08G005710 vs. NCBI nr
Match: gi|778728825|ref|XP_004135894.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 988.4 bits (2554), Expect = 4.9e-285
Identity = 492/542 (90.77%), Postives = 521/542 (96.13%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E N+TNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGV+IVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQIVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYYH  DVILCVLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR GAAI+IMQKF+IV+LLQL+EK+++SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGAAIMIMQKFDIVALLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AILGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD+ ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFV+KANGGAI+EE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. NCBI nr
Match: gi|659122577|ref|XP_008461216.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 987.3 bits (2551), Expect = 1.1e-284
Identity = 494/542 (91.14%), Postives = 517/542 (95.39%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E NQT EFIFRSKLPDIHIPNHLPLH YVFQN  K  +RPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNQTQEFIFRSKLPDIHIPNHLPLHQYVFQNMPKFATRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGVKIVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHFSVLSGADESLAPVVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYYH  DVIL VLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR GA ILIMQKF+IVSLLQL+EK+K+SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGATILIMQKFDIVSLLQLIEKHKISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFVVKANGGAITEE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL  GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLALGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 986.5 bits (2549), Expect = 1.9e-284
Identity = 492/542 (90.77%), Postives = 520/542 (95.94%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E N+TNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGD Y+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDFYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAAN KLIVTMACFYDRVKDLAENGV++VCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVQVVCVDFAVEGCLHFSVLSGADESLAPLVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DG+NPNLYYH  DVIL VLPFFHIYS
Sbjct: 181 NDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGENPNLYYHADDVILSVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR GAAILIMQKF+IVSLLQL+EK+++SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGAAILIMQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP A+LGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPMAVLGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFVVKANGGAITEE+V QFI++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 978.4 bits (2528), Expect = 5.1e-282
Identity = 489/542 (90.22%), Postives = 518/542 (95.57%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E N+TNEFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGDVY+YHDVQ
Sbjct: 1   MAFESNETNEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYTYHDVQ 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLHNLGIKKG+VVM+LLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHNLGIKKGDVVMHLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
           AKAA+ KLIVTMACFYDRVKDL ENGVKIVCVD+AVEGCLH+SVLSGADES  P VDF++
Sbjct: 121 AKAADAKLIVTMACFYDRVKDLGENGVKIVCVDFAVEGCLHFSVLSGADESLAPPVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYYH  DVILCVLPFFHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQMDGQNPNLYYHSNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLR G+AILI+QKF+IVSLLQL+EK+++SIMPIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRVGSAILIVQKFDIVSLLQLIEKHRISIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKELEDAVREKFP AILGQGYGMTEAGPVL+MSLAFAKEPF VKAGAC
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAILGQGYGMTEAGPVLSMSLAFAKEPFQVKAGAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVD ETGASLPANS+GEICIRG+QIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDTETGASLPANSSGEICIRGEQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVD++ ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDNDNELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFVVKA GGAITEE+V QFI++QVVFYKRLKRV FVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542

BLAST of CmaCh08G005710 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 956.1 bits (2470), Expect = 2.7e-275
Identity = 479/542 (88.38%), Postives = 505/542 (93.17%), Query Frame = 1

Query: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60
           MA E NQT+EFIFRSKLPDIHIPNHLPLH YVFQN SK  SRPCLINGATGDVYSY+DV 
Sbjct: 1   MAFESNQTHEFIFRSKLPDIHIPNHLPLHDYVFQNLSKFASRPCLINGATGDVYSYYDVN 60

Query: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120
           LTARRVA+GLH+LGIKKG+VVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ
Sbjct: 61  LTARRVAAGLHSLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180
            KAAN KLIVTMACFYDRVKDLAENGVKIVCVD++VEGCLH+SVLSGADES +P VDF++
Sbjct: 121 VKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFSVEGCLHFSVLSGADESLMPRVDFSS 180

Query: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240
           DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQ+DGQNPNLYY G DVILCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGQNPNLYYRGDDVILCVLPLFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300
           LNSILLCGLRAGAAI IMQKF+IVSL QL+ KYKVSI+PIVPPIFLAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAITIMQKFDIVSLFQLIGKYKVSIVPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360
           SVRVLKSGGAPLGKEL +AV+ KFPAAIL QGYGMTEAGPVLTMSLAFAKEPF VKA AC
Sbjct: 301 SVRVLKSGGAPLGKELVEAVKAKFPAAILAQGYGMTEAGPVLTMSLAFAKEPFQVKAEAC 360

Query: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420
           GTVVRNAEMKIVDPETGASLP NS GEICIRGDQIMKGYLNDLESTKRT+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPENSPGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480
           IGFVDD  ELFI+DRLKELIKFK FQVAPAELEALLI HPKL DAAVIGMPD EAGEVPV
Sbjct: 421 IGFVDDNNELFIIDRLKELIKFKTFQVAPAELEALLIAHPKLRDAAVIGMPDVEAGEVPV 480

Query: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540
           AFVV+   GA TEE+V QF+++QVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YN 543
           YN
Sbjct: 541 YN 542