CmaCh06G016810 (gene) Cucurbita maxima (Rimu)

NameCmaCh06G016810
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionDiphthamide biosynthesis protein 2
LocationCma_Chr06 : 10361541 .. 10367685 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAGCAGCTCATGAGAATCTAAGAAGAAGCTGTAATATCCAAATTGTACAATGTACTGAAGCTCTCTAGCTTCAATAAGGACGAACGACATACCTGAGACGGAGGATCGGTGCGGCTACCAACGAAGAGACCCAAAGAGCGACAAAGAGCGGGAGAGCAAATGATGGAGGGAGGCTCGAGCGATTGAGGTTTGGTTCCTGTAGTTTTCTTTTTTTAATTAAATTACAAATTTTATCGAAATAAAAATAAATTTTAAAAAAGGTTTTTTTTTTTAATTATTAGTTTAACCAAATATTGAATAAATTTATTTTGGGAATTATTTTGAATAATTTAATAAATTTATACGTTTAATAACCTTAATTCGAAAAGCTACCAAGTAAATTGCTGGCTGGAGTTGCAGGAAGTCCTCACGGCGGCCCAATTCTGCGAGAAGCGAGAGAGAGAGAGAACCGAGGATTCACCGGCCGTATCTCTGAACTCTGAATCTTCTTCTCTTTTATGGTCAGCAGCTGCTGAACCAGAATCTGCTCTTTCAACTTCTTCTACTTTCGCTATGGAGTTAGAATCATACTACGAAATTTCCCGCACCGCCGACTACATTCACACTCGGAACTTTACCAGAGTCGCATTGCAGGTATTACTACGAATCCACAAGTATTGCAGGTTTGAAATGATTTCAACTTTTTTATCTACTCCTTATTAACCAACTACGGAGAGTTTCGGTCTTGTGTTCTTCGAAATATTGTGGTTTGAGTTTGATTCAAGTCGAGTTTTTGCTCCCGTTCGGGTGCTATGAATGTACAAATGTTATATGTGTCATGGATTTTCTCATGTCTGGTTATTTGATCTTCAAATGCTCATAATTCCGTTTTTATATTATTATTTCCTACTTAATTGTTGATTTCTTTTATTTATGAAATTTTAGTTTCCTGATGAGCTCTTGAAGGATTCAACTAGAGTGGTCAGTGCTTTGAGGAAAAGACTCTGTGCGCTTAATGATCCAAACCAAAGCGATGACGAAAATAAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGCTGTTGCGTTGATGAGGTTGGGGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCGTGAGTATCAATCTACTATGGATATGTTATATGCTTTCTAGCAAGGTTTTTATGAACTTTTCACCTTGCAGTCCCCTTAGAAATCAGCATCCTAAGTTATTGACTTGTTTTTGCTAATTTGCGCAAGTGTTTGTGCTGTTGTTGATTTTTGCACTTTAGAGTGAGAAACTAAAATAACTAAAAAACTTGTAATCCTTGAGAAGTGGATTTCGGAGGTGTTTTCTGTCATATTAAATAATTGATAGGTGCGTTTGAATCCATTCTTTGAACTATCAGTATAATGTTTCAAATGTCTGAAATATCATTGACATGGTATAAAAATCTTAGATATTCACTGCAATACTATAATCCTTGGAGGTAGTATTATGAAATAAAGTATTTTATATTATCAATCATCATGTAAGAGGCATCTACATGGTTATGTTAATCTTTTGGATGATTGGCAGTCCCCGTTTCTCATGATTGAGAGAATAGGGTGGTGCTTGTTTGTCTGATCATAAGTGCACCTTCAGTAAAAATGGAGAAGTAATCGGGTGTTGGTCGTGATTTTAAGCCATATGATGTACTAAGGCGTTTGTATGCTTAGCTTTCCTTATGATAAGGAGTTGTGGATTACAGATTATTGCTTGCATGGTTTCTTATTTATTCCTCCTTGTTCGATGTTCTTGGTTGATTTCTTAGTTTAACTTTAATTTGAATATTAACATTTAGTTTCCTACTAAATGATTGGTAGATCAACAGATAGATAGTAAAGTTATAGCCTACCGTGCGTGCTATTATTTATATGATCCAATTTTTCAGACTGCATATAGCTTTTGAGGTCCATCACTTACAGTTATTGCTGGTTTACTATTAGCAGTGTTACAATACACTTTTGGTATCTTTCAGGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATCCAATCGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTCGTCCAATGGAAAGCCTGTTTTGGTATGCATTTGTGTTCTTAAATCACTGTACTTATTTTTATCTTTTTCGGGATAACTATATGAAATCCATTAAGAAGAATGAGGGAATGATTTAAAAAACAATACGATACAAAAACGTAAGCCTTAGGTTTCTTAGGCTTCCTTGTGGGTCTCTTTCTTTTTTGTATGCTCTTGTATTTTTTTCATTTTTTTCAATGAAAGTTAGATTTCTTACCAAAAAAAAAAAAAAAGAAAACTAGCTTGAGAATCCAAGTAAAGATAAAATGGGCTAAACTCCTCCACATCAATAAAAACACTCTTATGTCTTTCTAACTAAAAATACCACAAAATAGCTAAGAACCTAACTTGCTGAAGAAATCTCTCTTTGTTGTGAAAAGAGGGTTGGGGGAGGGGCAGATGGAGAATACAACATCAAAAACTTCATCTTTTATCGAAAACTCCTAATTTCGAAGAGGTTACAAACATGAAATCAAGTCCCATTAAAATGGCATTTGGATGGCTGATGAGTCTTTGATTTGAATTTCATTTGAAAAGACACCATCAGTGTAATTTGAAACACCTTTTGTGTGCCTCAAAAAGAAAGGCATCACCTATCTTTGCACTCCGGACTTTAGCCCGAAAAGACCATTCTGTTAACTTTTTCTGTATAATTAAGTTTGTTTTGGGTAATTCAAAGTTGCTTCCATGTGGTTCTTGGCTATAGGATTATCAATTAATGGCTTAATGCAAAGGGAAATAGAGTTTGAACTCTACATGCTTGCTTATTTGAGGTTTTCTTCTCGTTAGGTCCAGTACTGGCCTCAAACTTATGGAAATCAAGCTACTAGATAGATACTATTCATCCACTGTTCGTGTGTGTATATTTCTGTCAGTAGATTTCTATATCAAATATCAGATGCACACATAAAGGAATTTAAAATGTAGGAATCTCAAACATGAAAATATGTAGCTATTCACCCTTCCCCCCAATAAAATGTACTATTATTATTTTTCTTGGGGGGGGGGGGGGGGNTTTCTTAATCAAATCATAATTCAGTCGGACTATTTACTTCGACTGAGGTTGCAATTCTTTTTCTTTAGTGCTTTTTTTTTTCTTTTTTTCTTTTTTTCTTTTCACTTTGAAGCTTATACTCTAAACTCTAATAACGCTGCACTGGAGTTTGACAGTTTATCTAACTCCTTGAACAGGTTCTTTTTGGGTTGGAATATGCGCATTCAATGGATGATATTAGACAACCGCTATTAGATTCATTTCAGACAAGCAGTCTAACGTCAGAATTAGAAGTTCATTTTGCAGATGTTAAATGTTCAAGTTTGGATCCGTCCTCCCATAATGAAAATGTTGCCAGAGGTGGGGAACAAGTTACCAATGCTGAAAGTGGGAGTTTTGAGAATATTGCTGGAACCAGACATCACATTGGAAGCTTGTTTTGGGATTTACCTAGAGAGCGAAGAATGGAGGACTGCTCAATTTTCTGGATTGGTTCTGAGAATTCAGCCTTCGCAAATGTGGTTCTGACATTCAATGGCTGTGAGATAGGTGCGTAAATTGTTATGTTCTACATTGAGTTTATTTTATTAGCTACAACGTTAAATGTTCTAGTGTTTCCATTTCTTATGACGACATCTCAATTCGATTGCCCATTTTGAGAAATTTTGCTTGTCACAATAAGCTAACCCATAATTTTAAGCTCACATCTTGTCTTAACTTCATCGTTATTAATTTTAGTCAGATACGATGCAAAAGAAAGTTGCTTGGTTACTGATGTATCTCAGCAAAGAAGGATCCTCAAGCGCAGGTAATATTCGCCGTAAACTCATTCATTATCCACTTTTGTTCCCTTTAAACTGATTTTTGTCAATATATTTGTAAGATAGCATGCCTTCTTTTGTTGAGATTACATTCTCCAGTAATACAATGCGTTCATTTTTTTTCTTTTATTTTTGGGATAATGAAACTGGATACTGTAATCGTCTTTTGTTTTATTTACTTTTATCTTATTATGTGTCAATCAGATATTACTTGGTGGAGAAGGCAAAGGATGCTGCCATTGTTGGAATTTTGGTTGGTACTCTTGGTTTAGGTATGTATTGACAAAAACTGACGGAAATCATCTGCAAACAAAAACTGGACATGTCATCTTCTTAATCCGTTTACGGCGTATTGGATCTTTCTTTGTTGAGGACCAGAGGAGCATTGTTGTATCGTATTAATTTGTGAATTTTAAAAGGCGTTTTAATTTGGTGATGATTTATGTAGCTGGTTACCTCCATATCATTCATCAGATGAAAGAGCTGATCACTGGAGCAGGGAAGAAGGCTTATACTCTTGTTATGGGAAGACCCAATCCTGCAAAACTTGCCAACTTCCCAGAGGTATTTTTGTCAGACTCATGCGACTTAATTTGCTTTACTGGTAGTCTGATTCCTTAGCATTTCTTGCAGTGTGGTGTTTTCATATATGTTTCTTGTGCCCAAACTGCACTTATGGATAGCAAGGAGTATCTTGCTCCTGTCATTACTCCATTTGAAGCCACACTAGCTTTCACCAGGTATTTCTAATCTTTGTCTTGTTTCATCTTTAATTTCTCGAGATCATGTAACGTGAGCTGTCTTAGTTCTGTTAACTCCGTGCCAAAATCGATTCATGTCGTCATGAAGTCTAGTTGGAACCTTACTTCTTTATATGATCTTATGGCTAGCTGTTCAATCATGCATAGAAATATTGTCAAGTTGTTGAAGAATTCGCCCCGGGCTACATAAAGTAAGATGATGGCCACCCGCATTCTGAAATGGGTCACAACTACTATACCAATTGGTTTGAAAAACCTATGTCTTAGTTAGGAAATAGCCTTGCAGGGAAGTATCCTCGCTTTGACACTTCGTTTAGAAACCATGACTCGAAAATCTTATTGTTGAATATGCCATTTCATTTTCCAATATGCTGGTGAATCTATGTGATTCTAAATTTGAACTGAGGGATGCATATGATTATATCAATTGAACGATCAAATGGAGAAACCATCATTTTCTTCAGATGGAGAATAAATAGTTTATATTTTAGATCTTATCTTTAAATTTCATTTGGATCTTAATTGAAGGATGGAAATTTGAATTGTCCAGAGGAAGTCAATGGACAGGTGCCTATGTTATGGAATTTCAGGATTTGATTAATTTCTCGATACCTAAAGAAGGAAACCGATCAGATGAAGCGAGATATTCGTTCTTGCAAGGCAGATATGTTGAAGATTATGATTCCCAAGGTACTGACCCTTCACGCCCTTATGTTTTCATGCTGCAAGAGTTCTTTTGGTTAGAGTTATATAACGTTTCCTTTAAAATCTCCTCTTATTCGTACTTTCAAAAATCTCTCTGCCTCCATTTCCTAAAACCTTCTTTTCAAATGAAACCAGAGGCTTGAGCATATGTTGCCCGGCCGTAGGCTTGCATTAGTTACAAGTTCTATCACTTCACTATATTAACTTCCAGCATCACAGTTTTGATTCTGAGATTGTTCTTAATTTGTTTTCTAAACAGAAAATGTTGAGGAAGAAAATGAAGCTTGCACTCTAGTAAGTGCGACAGAGAAAGCTCTCCAGATACGCGATAACCGAAACTCGCTAATTGAAGGAACTGCGAGGTCTGGAGCGGAATTTTTTGCAGCTCGTTCCTTTCAAGGTTTAGATATTCAAAATGGTAGTTCCCAGCCGGAGCCATATCTAATCGGGAGGAGCGGCAGGGCATCAGGGTATCAAGACGAGAAAAATAGTCATTAAGAGAAGGTTTGAAGATTGAAGCAAGTTTGTAAGGGTCAGTCGGAGAGATTCCAGCAAGCTTGGGAAAAGAGGTTGGTTGTCAATCTTACAAAGAGATTTTGTTTCACTAGGTTTGTTCGGAAGAGTGTTCTATTTTTTATTAGTGTTTCTAAAAGATCATGAGAAATGAATGTTAAACATTAATATTATTGTGCACAGTGAAATTTCTATTTAGAAGGAAGAAC

mRNA sequence

ATGGAAGAAGCAGCTCATGAGAATCTAAGAAGAAGCTGTAATATCCAAATTGAAGTCCTCACGGCGGCCCAATTCTGCGAGAAGCGAGAGAGAGAGAGAACCGAGGATTCACCGGCCGTATCTCTGAACTCTGAATCTTCTTCTCTTTTATGGTCAGCAGCTGCTGAACCAGAATCTGCTCTTTCAACTTCTTCTACTTTCGCTATGGAGTTAGAATCATACTACGAAATTTCCCGCACCGCCGACTACATTCACACTCGGAACTTTACCAGAGTCGCATTGCAGTTTCCTGATGAGCTCTTGAAGGATTCAACTAGAGTGGTCAGTGCTTTGAGGAAAAGACTCTGTGCGCTTAATGATCCAAACCAAAGCGATGACGAAAATAAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGCTGTTGCGTTGATGAGGTTGGGGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATCCAATCGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTCGTCCAATGGAAAGCCTGTTTTGGTTCTTTTTGGGTTGGAATATGCGCATTCAATGGATGATATTAGACAACCGCTATTAGATTCATTTCAGACAAGCAGTCTAACGTCAGAATTAGAAGTTCATTTTGCAGATGTTAAATGTTCAAGTTTGGATCCGTCCTCCCATAATGAAAATGTTGCCAGAGGTGGGGAACAAGTTACCAATGCTGAAAGTGGGAGTTTTGAGAATATTGCTGGAACCAGACATCACATTGGAAGCTTGTTTTGGGATTTACCTAGAGAGCGAAGAATGGAGGACTGCTCAATTTTCTGGATTGGTTCTGAGAATTCAGCCTTCGCAAATGTGGTTCTGACATTCAATGGCTGTGAGATAGTCAGATACGATGCAAAAGAAAGTTGCTTGGTTACTGATGTATCTCAGCAAAGAAGGATCCTCAAGCGCAGATATTACTTGGTGGAGAAGGCAAAGGATGCTGCCATTGTTGGAATTTTGGTTGGTACTCTTGGTTTAGCTGGTTACCTCCATATCATTCATCAGATGAAAGAGCTGATCACTGGAGCAGGGAAGAAGGCTTATACTCTTGTTATGGGAAGACCCAATCCTGCAAAACTTGCCAACTTCCCAGAGTGTGGTGTTTTCATATATGTTTCTTGTGCCCAAACTGCACTTATGGATAGCAAGGAGTATCTTGCTCCTGTCATTACTCCATTTGAAGCCACACTAGCTTTCACCAGAGGAAGTCAATGGACAGGTGCCTATGTTATGGAATTTCAGGATTTGATTAATTTCTCGATACCTAAAGAAGGAAACCGATCAGATGAAGCGAGATATTCGTTCTTGCAAGGCAGATATGTTGAAGATTATGATTCCCAAGAAAATGTTGAGGAAGAAAATGAAGCTTGCACTCTAGTAAGTGCGACAGAGAAAGCTCTCCAGATACGCGATAACCGAAACTCGCTAATTGAAGGAACTGCGAGGTCTGGAGCGGAATTTTTTGCAGCTCGTTCCTTTCAAGGTTTAGATATTCAAAATGGTAGTTCCCAGCCGGAGCCATATCTAATCGGGAGGAGCGGCAGGGCATCAGGGTATCAAGACGAGAAAAATAGTCATTAAGAGAAGGTTTGAAGATTGAAGCAAGTTTGTAAGGGTCAGTCGGAGAGATTCCAGCAAGCTTGGGAAAAGAGGTTGGTTGTCAATCTTACAAAGAGATTTTGTTTCACTAGGTTTGTTCGGAAGAGTGTTCTATTTTTTATTAGTGTTTCTAAAAGATCATGAGAAATGAATGTTAAACATTAATATTATTGTGCACAGTGAAATTTCTATTTAGAAGGAAGAAC

Coding sequence (CDS)

ATGGAAGAAGCAGCTCATGAGAATCTAAGAAGAAGCTGTAATATCCAAATTGAAGTCCTCACGGCGGCCCAATTCTGCGAGAAGCGAGAGAGAGAGAGAACCGAGGATTCACCGGCCGTATCTCTGAACTCTGAATCTTCTTCTCTTTTATGGTCAGCAGCTGCTGAACCAGAATCTGCTCTTTCAACTTCTTCTACTTTCGCTATGGAGTTAGAATCATACTACGAAATTTCCCGCACCGCCGACTACATTCACACTCGGAACTTTACCAGAGTCGCATTGCAGTTTCCTGATGAGCTCTTGAAGGATTCAACTAGAGTGGTCAGTGCTTTGAGGAAAAGACTCTGTGCGCTTAATGATCCAAACCAAAGCGATGACGAAAATAAGGTTAGGTTGTTCATAATGGCTGATACAACTTACGGTAGCTGTTGCGTTGATGAGGTTGGGGCAGCTCACGCTAATGCTGATTGTGTCATTCACTATGGACATACATGTCTGAGCCCGACAACAACTCTTCCAGCATTTTTTGTTTTCGGAAAGGATCCAATCGATGTCTCCACTTGTGCCAAAACTTTATCTCACTACAGTTCGTCCAATGGAAAGCCTGTTTTGGTTCTTTTTGGGTTGGAATATGCGCATTCAATGGATGATATTAGACAACCGCTATTAGATTCATTTCAGACAAGCAGTCTAACGTCAGAATTAGAAGTTCATTTTGCAGATGTTAAATGTTCAAGTTTGGATCCGTCCTCCCATAATGAAAATGTTGCCAGAGGTGGGGAACAAGTTACCAATGCTGAAAGTGGGAGTTTTGAGAATATTGCTGGAACCAGACATCACATTGGAAGCTTGTTTTGGGATTTACCTAGAGAGCGAAGAATGGAGGACTGCTCAATTTTCTGGATTGGTTCTGAGAATTCAGCCTTCGCAAATGTGGTTCTGACATTCAATGGCTGTGAGATAGTCAGATACGATGCAAAAGAAAGTTGCTTGGTTACTGATGTATCTCAGCAAAGAAGGATCCTCAAGCGCAGATATTACTTGGTGGAGAAGGCAAAGGATGCTGCCATTGTTGGAATTTTGGTTGGTACTCTTGGTTTAGCTGGTTACCTCCATATCATTCATCAGATGAAAGAGCTGATCACTGGAGCAGGGAAGAAGGCTTATACTCTTGTTATGGGAAGACCCAATCCTGCAAAACTTGCCAACTTCCCAGAGTGTGGTGTTTTCATATATGTTTCTTGTGCCCAAACTGCACTTATGGATAGCAAGGAGTATCTTGCTCCTGTCATTACTCCATTTGAAGCCACACTAGCTTTCACCAGAGGAAGTCAATGGACAGGTGCCTATGTTATGGAATTTCAGGATTTGATTAATTTCTCGATACCTAAAGAAGGAAACCGATCAGATGAAGCGAGATATTCGTTCTTGCAAGGCAGATATGTTGAAGATTATGATTCCCAAGAAAATGTTGAGGAAGAAAATGAAGCTTGCACTCTAGTAAGTGCGACAGAGAAAGCTCTCCAGATACGCGATAACCGAAACTCGCTAATTGAAGGAACTGCGAGGTCTGGAGCGGAATTTTTTGCAGCTCGTTCCTTTCAAGGTTTAGATATTCAAAATGGTAGTTCCCAGCCGGAGCCATATCTAATCGGGAGGAGCGGCAGGGCATCAGGGTATCAAGACGAGAAAAATAGTCATTAA

Protein sequence

MEEAAHENLRRSCNIQIEVLTAAQFCEKRERERTEDSPAVSLNSESSSLLWSAAAEPESALSTSSTFAMELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVEDYDSQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQPEPYLIGRSGRASGYQDEKNSH
BLAST of CmaCh06G016810 vs. Swiss-Prot
Match: DPH2_DANRE (Diphthamide biosynthesis protein 2 OS=Danio rerio GN=dph2 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.1e-77
Identity = 178/527 (33.78%), Postives = 275/527 (52.18%), Query Frame = 1

Query: 51  WSAAAEPESALSTSSTFAM----ELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTR 110
           +S+++E     S +ST +     +L   Y+   T  +I + +F +VALQFPDELL D+ R
Sbjct: 5   FSSSSEAVLQRSVTSTHSFHPSGDLLLLYQTPETCRFITSNHFKKVALQFPDELLPDAVR 64

Query: 111 VVSALRKRLCALNDPNQSDDENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCL 170
           V + +             +D+ K + +I+ DT+YGSCCVDEV A H  ADC++HYG +CL
Sbjct: 65  VSAEI-------------EDKTKAKTYILGDTSYGSCCVDEVAAEHVGADCIVHYGSSCL 124

Query: 171 SPTTTLPAFFVFGKDPIDVSTCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSF 230
           SP   LP  +VFGK PIDV  CA +      +    ++VLF + Y+H++DD+R  L D +
Sbjct: 125 SPCRRLPLLYVFGKRPIDVHQCASSFKELYPNLQSHIIVLFDVTYSHAIDDLRTLLCDVY 184

Query: 231 QTSSLTSELEVHFADVKCSSLDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFW 290
             + + S L+    D  C              G E + ++      N  G     G  F 
Sbjct: 185 P-NVVVSRLKT---DHSC--------------GAELIQDSCVDLQSNDDGVIFKFGRQF- 244

Query: 291 DLPRERRMEDCSIFWIGSENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRY 350
            +   + + D SIF+IG E     N ++++N C    ++ + S    +  Q  + L +RY
Sbjct: 245 RIKEGQTVNDYSIFYIGQEGLTLTNFMMSWNNCVFSSFNPETSTGRVESVQINKALMKRY 304

Query: 351 YLVEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPE 410
           Y +E+AKDA++VGILVGTLG+A YL II Q+K+ I  AGKK+Y   MG+ N  KLANF E
Sbjct: 305 YAIERAKDASVVGILVGTLGVANYLIIIEQLKDTIQRAGKKSYMFAMGKINVPKLANFLE 364

Query: 411 CGVFIYVSCAQTALMDSKEYLAPVITPFEATLAFTRGSQWTGAYVMEFQDL-------IN 470
             +++ V+C + +L+DS E+  PV+TPFE  LA  +  +WTG YV +F++L       + 
Sbjct: 365 IDIYVLVACPENSLLDSSEFYRPVVTPFEMELACNKHREWTGEYVTDFRELLPGGSSHVG 424

Query: 471 FSIPKEGNRSDEAR-YSFLQGRYVEDYDSQENVEEENEACTLVSATEKALQIRDNRNSLI 530
           F  P +    +E    S + G       +   +   +E  +LV   +          +L 
Sbjct: 425 FPEPSQSATEEETTDVSLITGALRSCSTNSSEMMHNSETSSLVLRNQ----------TLT 484

Query: 531 EGTARSGAEFFAARSFQGLDIQNGSSQPEPYLIGRSGRASGYQDEKN 566
                + A F A RS+QGL+ + G +     + G+ G A  Y++E N
Sbjct: 485 VANTNAAASFLAGRSWQGLEPKLGQTPVVKAVKGQRGIAIAYEEEGN 489

BLAST of CmaCh06G016810 vs. Swiss-Prot
Match: DPH2_CHICK (Diphthamide biosynthesis protein 2 OS=Gallus gallus GN=DPH2 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 8.2e-73
Identity = 164/498 (32.93%), Postives = 252/498 (50.60%), Query Frame = 1

Query: 72  ESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVR 131
           + +YE+ R A ++    F +VALQFPD LL D+  V + +             ++     
Sbjct: 29  DEFYEVDRAAAFVRDGGFRKVALQFPDALLADAAAVAARM-------------EEVTGAE 88

Query: 132 LFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTCAKT 191
           ++++ DTTYGSCCVDEV A H +A  V+HYG  CLSP   LP   VFG+ P+DV  CA+ 
Sbjct: 89  MYVLGDTTYGSCCVDEVAAEHVSAGAVVHYGPACLSPCRKLPVLHVFGRQPLDVGRCAEV 148

Query: 192 LSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLDPSS 251
                      V+VL  + YAH+M ++ + L   +          + F++V C       
Sbjct: 149 FRELYPERQSRVVVLSDVVYAHAMGELEKQLCHEYPN--------IIFSEVVC------- 208

Query: 252 HNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSENSAFAN 311
                   G+  +    G      G R H+ +          ++DCS+F++G+E  A  +
Sbjct: 209 --------GDAPSPTLPGEVRQF-GRRFHMEAA-------EELQDCSMFYVGAEGLALTS 268

Query: 312 VVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLGLAGYL 371
            +LT+N      +D        +     R L RR YLVE+A+DA +VGILVGTLG+AGYL
Sbjct: 269 FMLTWNRFPFSSFDPATGHGRRETLNVNRALMRRLYLVERARDAHVVGILVGTLGVAGYL 328

Query: 372 HIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEYLAPVI 431
            ++  + +L+  AGK++YTL +G+PNPAKLANF E  +F+ V+CAQ +L+DS E+  P++
Sbjct: 329 DVLEHLHQLVRRAGKRSYTLSVGKPNPAKLANFLEVDIFVLVACAQNSLLDSSEFYRPIV 388

Query: 432 TPFEATLAFTRGSQWTGAYVMEFQDLI-----NFSIPKEGNRSDE-ARYSFLQGRYVEDY 491
           TP+E  LA     +WTG Y+ +F+DL+     +  +P     ++     S + G     +
Sbjct: 389 TPYELELACNPAREWTGNYLTDFRDLLPGACAHIELPPAVPAAEAIPDVSLITGEMRATH 448

Query: 492 DSQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQ 551
                  +   + TL          RD   +L E      A F  +RS++GL+ Q G + 
Sbjct: 449 LCDPLAPQPPSSTTLA--------CRDQTRALAE--MSPAATFLESRSWRGLEQQLGKTA 472

Query: 552 PEPYLIGRSGRASGYQDE 564
               + GR G A  Y+DE
Sbjct: 509 VSKAVQGRRGIAIAYEDE 472

BLAST of CmaCh06G016810 vs. Swiss-Prot
Match: DPH2_NEMVE (Diphthamide biosynthesis protein 2 OS=Nematostella vectensis GN=dph2 PE=3 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 8.4e-70
Identity = 179/565 (31.68%), Postives = 278/565 (49.20%), Query Frame = 1

Query: 36  DSPAVSLNSESSSLLWSAAAEPESALSTSSTFAME-LESYYEISRTADYIHTRNFTRVAL 95
           ++ A S +  +S+   SA  +    + ++ T ++E +   YEI R+   I +  F +VAL
Sbjct: 4   ETTAKSYDVPASAFSESAVIDTHLDVPSTKTESIERMVEVYEIERSVQVITSSGFNKVAL 63

Query: 96  QFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLFIMADTTYG------------- 155
           QFPD LL DS  V   + +R                + FI+ADT+YG             
Sbjct: 64  QFPDSLLADSASVARLIEQRASC-------------KAFILADTSYGRHPPLPANKTFLK 123

Query: 156 -----------------SCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPID 215
                            SCCVDE+ A HA+A+ +IHYG  CLS T  LP  +VFGK+PI+
Sbjct: 124 LYNVRICFKLLAFLHFSSCCVDEIAAEHADAELIIHYGQACLSQTKRLPVLYVFGKNPIN 183

Query: 216 VSTCAKTLSHYSSSNGKPVLVLFGLEYAH---SMDDIRQPLLDSFQTSSLTSELEVHFAD 275
           V  C++         G  VLV + + Y H   ++D+   PL  +   S++  E       
Sbjct: 184 VIECSQHFRQLYPDTGIRVLVFYDVVYNHCIGALDEALSPLYPNMTISTIAPEGL----- 243

Query: 276 VKCSSLDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFW 335
                  PS      +    Q ++ E      +       G  F  L     + D  IF+
Sbjct: 244 -------PSEPTSQQSSRNPQASDVEG---MEVNQCNRRFGRDF-TLAANSSISDYQIFY 303

Query: 336 IGSENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGIL 395
           IG ++    N+++T+N C+   YD   +    +     + L +RY+++++AKDA IVGI+
Sbjct: 304 IGEQSLTLRNLMMTYNKCQFSTYDPITNESRRETLNVNKALMKRYHMIQRAKDAQIVGIV 363

Query: 396 VGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALM 455
           VGTLG+A YL II ++K+++  AGKK+Y  VMG+ N AKLANF E  VF+ VSC + +L+
Sbjct: 364 VGTLGVADYLKIIERLKKVLAIAGKKSYVFVMGKLNVAKLANFLEIDVFVLVSCPENSLI 423

Query: 456 DSKEYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLI---NFSIPKEGNRSDEARYSFLQ 515
           DSKE+  PV+TP+E  +A  R  +WTG YV +F +L+   + +     +  D    S + 
Sbjct: 424 DSKEFYKPVVTPYEMEIACLRTQEWTGDYVTDFHELLPGKSINTFWTDDNDDSPYISLIT 483

Query: 516 GRYVEDYDSQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLD 564
           G+   +Y S  + +E  E  T +        ++ N+ + +       AEF A+RS+QGL 
Sbjct: 484 GKMQHNYKS--SAKEAGETSTSL--------VQRNQETTLATQQPLTAEFLASRSWQGLQ 529

BLAST of CmaCh06G016810 vs. Swiss-Prot
Match: DPH2_SCHPO (Diphthamide biosynthesis protein 2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=dph2 PE=3 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 1.0e-67
Identity = 163/540 (30.19%), Postives = 273/540 (50.56%), Query Frame = 1

Query: 35  EDSPAVSLNSESSSLLWSAAAEPESALSTSSTFAMELESYYEISRTADYIHTRNFTRVAL 94
           E S  V L+  S ++L       E  +  +     +L   YEI+RT D+I + N+  VAL
Sbjct: 4   ELSAPVPLSDNSEAIL-------EKEIGETVKIEGDLVEVYEINRTVDFIKSGNYNSVAL 63

Query: 95  QFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLFIMADTTYGSCCVDEVGAAHAN 154
           QFPDE L DS +V S L   +             +  + I+ADT YGSCCVDEV A H +
Sbjct: 64  QFPDEHLADSGKVASILTNLV-------------EANVQILADTNYGSCCVDEVAAEHMS 123

Query: 155 ADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTCAKTLSHYSSSNGKPVLVLFGLEYAHS 214
           AD ++HYG  CLSPT+ LP  +VFG+ PI++    K L+     N   +L++    + ++
Sbjct: 124 ADAIVHYGRACLSPTSRLPVLYVFGRLPINLHKLEKCLTIPLDQN---ILLVSDTRWYYA 183

Query: 215 MDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLDPSSHNENVARGGEQVTNAESGSFENI 274
            D I    L S +T    +  E H  +     ++P               N E       
Sbjct: 184 QDSI----LKSLKTLGYQNVYESHLKE----RIEP---------------NLEE------ 243

Query: 275 AGTRHHIGSLFWDLPRERRMEDCSIFWIGSENSAFANVVLTFNGC--EIVRYDAKESCLV 334
           A T + I    + LP+   ++D ++ +IG ++   ++++++      + + +D   + +V
Sbjct: 244 ASTSYTIPGRTYSLPKSLSLQDMTLLYIGPDSPTLSSILMSHYSLVNQFLSFDPLSNKIV 303

Query: 335 TDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLV 394
            + S     L+RRY LV++ +DA ++GI++GTLG+  YLH+++Q++++I  AGKK Y L 
Sbjct: 304 EESSFTGAKLRRRYALVQRCRDAGVIGIVIGTLGVHRYLHVLNQLRKMILNAGKKPYMLA 363

Query: 395 MGRPNPAKLANFPECGVFIYVSCAQTALMDSKEYLAPVITPFEATLAFTRGSQWTGAYVM 454
           +G+ NPAKLANF E   F+ ++C + +L+DSKE+  P++TPFE   A +    W   +++
Sbjct: 364 VGKLNPAKLANFQEIECFVLIACGENSLIDSKEFYRPIVTPFELVKALSSDMSWNNDFIL 423

Query: 455 EFQDLINFSIPKEG--------NRSDEARYSFLQGRYVEDYDSQE-NVEEENEACTLVSA 514
            F +++  S  K+           S E  +S + G++V     +  +V  E        +
Sbjct: 424 SFDEVLKLSEGKQSKEPSEVLTEESAEPHFSLITGKFVNSTPMRHLDVTLETADAKNNDS 483

Query: 515 TEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQPEPYLIGRSGRASGYQDE 564
           +  +++ R  R+  + G     A F  ++S+ GLD  +    P     G+SG A GY  E
Sbjct: 484 SSASIEKRGMRSLAVNGVYSPAAAFLQSKSWSGLDSVDEGEGPSKLYEGQSGIAKGYVGE 491

BLAST of CmaCh06G016810 vs. Swiss-Prot
Match: DPH2_XENLA (Diphthamide biosynthesis protein 2 OS=Xenopus laevis GN=dph2 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.3e-62
Identity = 165/507 (32.54%), Postives = 253/507 (49.90%), Query Frame = 1

Query: 70  ELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENK 129
           +L  +YEI +T ++I      +VALQFPD+LL DS +V   L             ++   
Sbjct: 32  DLGEFYEIEKTVEFIQRNAAQKVALQFPDDLLLDSVKVARKL-------------EEATG 91

Query: 130 VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTCA 189
            + +I+ DT+YGSCCVDEV A H  A+ ++HYG  CLSP   LP  +VFG+  +++  CA
Sbjct: 92  AKTYILGDTSYGSCCVDEVAAEHVKANVLVHYGRACLSPCCRLPVSYVFGRKAVNMDLCA 151

Query: 190 KT-LSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQT---SSLTSELEVHFADVKCS 249
           +  LSHY  +    V+VL  + Y H++ ++ + +  ++     S LTS          C 
Sbjct: 152 EAFLSHYRDTESH-VVVLSDVVYDHALGELAKRIRSAYPNVIFSKLTS----------C- 211

Query: 250 SLDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSE 309
                         GE  +  E   F    G R       W        E   IF++G E
Sbjct: 212 --------------GETASPDEIVKF----GRRFSPDLRLWP-------ESYGIFYVGGE 271

Query: 310 NSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTL 369
            S   N++LT+  C    ++       T+     R L  R+YL+E+A+DA + GILVGTL
Sbjct: 272 GSTLNNLMLTWPRCSFFSFNPFTGEGRTEGLHVNRALMIRFYLIERARDAHVFGILVGTL 331

Query: 370 GLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKE 429
           G++ YL  +  +K +I  AGKK+Y   +G+ NPAKLANFPE  VF+ V+C + +L+DS E
Sbjct: 332 GVSDYLSALKHLKNIIHLAGKKSYMFSVGKLNPAKLANFPEIDVFVLVACPENSLLDSSE 391

Query: 430 YLAPVITPFEATLAFTRGSQWTGAYVMEFQDL-------INF--SIPKEGNRSDEARYSF 489
           +  PV+TP E  +A     +W G  +  F++L       + F  + P + + +D    S 
Sbjct: 392 FYKPVVTPDEMEIACNPAREWHGYCITNFRELLPGGSAYVEFPETDPSDAHHTD---VSL 451

Query: 490 LQGRYVEDYDSQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQG 549
           + G       S   V E     TL   ++ +L  R+++ +L +    S A + A+RS+QG
Sbjct: 452 ITGNL---RSSHLTVAE-----TLEKDSDTSLVQRNSKTALAQ--MSSAASYLASRSWQG 475

Query: 550 LDIQNGSSQPEPYLIGRSGRASGYQDE 564
           LD   G +     + GR G A  Y+DE
Sbjct: 512 LDKALGQTPVVKAVEGRKGIAIAYEDE 475

BLAST of CmaCh06G016810 vs. TrEMBL
Match: A0A0A0KW05_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011710 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 3.7e-250
Identity = 435/499 (87.17%), Postives = 464/499 (92.99%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALN--DPNQSDD 128
           ME ESYYEISRTAD+IHTRNFTRVALQFPDELLKDSTRVV AL+ RL  L+  D N SD 
Sbjct: 1   MEFESYYEISRTADFIHTRNFTRVALQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDS 60

Query: 129 ENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVS 188
           +++VRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPA FVFGKD IDV 
Sbjct: 61  KSEVRLFVMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPALFVFGKDSIDVP 120

Query: 189 TCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSS 248
           TCAKTLS YS S+GKPVLVLFGLEYAHSM DI+Q LLDSFQTSSL SELEVHFADVKCSS
Sbjct: 121 TCAKTLSQYSLSSGKPVLVLFGLEYAHSMLDIKQRLLDSFQTSSLGSELEVHFADVKCSS 180

Query: 249 LDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSEN 308
           LDPS H+ENV +GGEQ +NAESGS E+IAG RHHIG LFW+LP+ERR++DCS+FWIGSEN
Sbjct: 181 LDPSPHHENVVKGGEQASNAESGSSESIAGARHHIGGLFWELPKERRVKDCSLFWIGSEN 240

Query: 309 SAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLG 368
           SAFANVVLTFNGCEIVRYDAKES LVTDVS+QRRILKRRYYLVEKAKDAAIVGILVGTLG
Sbjct: 241 SAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYLVEKAKDAAIVGILVGTLG 300

Query: 369 LAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEY 428
           LAGYLHIIHQMKELITGAGKKAYTLVMG+PNPAKLANFPECGVFIYVSCAQTALMDSKEY
Sbjct: 301 LAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECGVFIYVSCAQTALMDSKEY 360

Query: 429 LAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVEDYD 488
           LAPVITPFEATLAF+RGSQWTGAYVMEFQDLI+FS P+E NRSDEARYSFLQG YVED+D
Sbjct: 361 LAPVITPFEATLAFSRGSQWTGAYVMEFQDLIDFSTPEEANRSDEARYSFLQGGYVEDWD 420

Query: 489 SQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQP 548
           SQEN EEEN A  LV+ATEKALQ+RDNRNSLIEGTARSGAEFFA RSFQGLDI NGS +P
Sbjct: 421 SQENTEEENGAHALVTATEKALQLRDNRNSLIEGTARSGAEFFATRSFQGLDINNGSLEP 480

Query: 549 EPYLIGRSGRASGYQDEKN 566
           EPY+IGRSG+ASGYQDEKN
Sbjct: 481 EPYVIGRSGKASGYQDEKN 499

BLAST of CmaCh06G016810 vs. TrEMBL
Match: B9SX45_RICCO (Diphteria toxin resistance protein 2, dph2, putative OS=Ricinus communis GN=RCOM_0547500 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 2.2e-186
Identity = 336/501 (67.07%), Postives = 402/501 (80.24%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDEN 128
           M+ E  YEI  T D+IH+RNFTRVALQFPDELLKDS RVVSALR +L  L +     D  
Sbjct: 1   MDFEENYEIDLTTDFIHSRNFTRVALQFPDELLKDSVRVVSALRNKLELLEE--NGGDRK 60

Query: 129 KVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTC 188
           ++RLF+MADT YGSCCVDEVGA+HANADCVIHYGHTCLSPT+ LPAFFVFGK  I+VS+C
Sbjct: 61  EIRLFVMADTAYGSCCVDEVGASHANADCVIHYGHTCLSPTSNLPAFFVFGKASINVSSC 120

Query: 189 AKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLD 248
           A++LS Y+ S+ KP++VL+GLEYAH++  IRQ L+ +    S     E+HFADV CS+++
Sbjct: 121 AQSLSDYAFSSTKPIMVLYGLEYAHAIPHIRQELIAAASMQSRPRHSELHFADVMCSAIN 180

Query: 249 PSSH----NENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGS 308
           PS++    +E     G+ V   + G     AG RH IG L WDL R +RMED  + WIGS
Sbjct: 181 PSNNQKKSDEPARLIGDCVATNDVGE---AAGCRHKIGGLIWDLSRGQRMEDYLLVWIGS 240

Query: 309 ENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGT 368
            NSAFANVVLTFNGCEIVRYDAKES LVTD+SQQRRILKRRYYLVEKAKDA IVGILVGT
Sbjct: 241 GNSAFANVVLTFNGCEIVRYDAKESRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGT 300

Query: 369 LGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSK 428
           LG+AGYL +IHQMK+LIT AGKKAYTLVMGRPNPAKLANFPEC +FIYVSCAQTAL+DSK
Sbjct: 301 LGVAGYLDMIHQMKDLITSAGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSK 360

Query: 429 EYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVED 488
           E+LAPVITPFEA +AF RGSQWTGAYVMEF+DLI+ S  +   +  EAR+SF+QGRYVED
Sbjct: 361 EFLAPVITPFEAMIAFNRGSQWTGAYVMEFRDLIDSSPVEAMIKPAEARFSFIQGRYVED 420

Query: 489 YDSQENVEEENEACTLVSATEKALQIRD-NRNSLIEGTARSGAEFFAARSFQGLDIQNGS 548
           +D QEN+EE+NE   L +ATEKALQ+ D N  SLI+GT +SGAE+FA RS+QGL++   +
Sbjct: 421 FDLQENIEEDNEGLALANATEKALQLHDRNSTSLIKGTIKSGAEYFANRSYQGLEMHGDN 480

Query: 549 SQPEPYLIGRSGRASGYQDEK 565
           S+P  +LIGR+G+ASGY++EK
Sbjct: 481 SKPGAFLIGRTGKASGYENEK 496

BLAST of CmaCh06G016810 vs. TrEMBL
Match: A0A067L745_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05728 PE=3 SV=1)

HSP 1 Score: 650.6 bits (1677), Expect = 1.8e-183
Identity = 331/501 (66.07%), Postives = 399/501 (79.64%), Query Frame = 1

Query: 68  AMELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDP-NQSDD 127
           AM+ E  YEI+RTAD+IH+RNFTRVALQFPDELLKDS RVVSALR +L +L     +   
Sbjct: 2   AMDFEENYEINRTADFIHSRNFTRVALQFPDELLKDSVRVVSALRDKLQSLRASIAEKIG 61

Query: 128 ENK-VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDV 187
           ENK  RLF+MADTTYGSCCVDEVGA+H +ADCVIHYGHTCLSPT+TLPAFFVFGK  I +
Sbjct: 62  ENKEARLFVMADTTYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPAFFVFGKASISI 121

Query: 188 STCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCS 247
            +CA++LS Y+S+N KP++V++GLEYAH++  I++  + +    S     +  FADV CS
Sbjct: 122 HSCAQSLSDYASTNSKPIVVIYGLEYAHAIPHIKEKFMAASSIESGPKHSQSLFADVMCS 181

Query: 248 SLDPSSHNENVARGGEQVTNAE-SGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGS 307
            ++P   ++N     + + +   S   +   G++H IG L W+LP  RRMED  + WIGS
Sbjct: 182 VINPFDSHKNSDGLPKLLGDYNASNDVDAAVGSKHKIGGLIWELPNGRRMEDYLLVWIGS 241

Query: 308 ENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGT 367
           ++SAFANVVLTFNGCEIVRYDAKE+ LVTD+SQQRRILKRRYYLVEKAKDA IVGILVGT
Sbjct: 242 DSSAFANVVLTFNGCEIVRYDAKENRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGT 301

Query: 368 LGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSK 427
           LG+AGYL +IHQMK+LIT AGKK YTLVMGRPNPAKLANFPEC VFIYVSCAQTAL+DSK
Sbjct: 302 LGVAGYLDMIHQMKDLITNAGKKTYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSK 361

Query: 428 EYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVED 487
           E+LAPVITPFEA +AF RGS WTG YVMEF+DLIN S  +  N S+EAR+SF+QGRYVED
Sbjct: 362 EFLAPVITPFEAMVAFNRGSLWTGEYVMEFRDLINSSPAEARNNSEEARFSFMQGRYVED 421

Query: 488 YDSQENVEEENEACTLVSATEKALQIRD-NRNSLIEGTARSGAEFFAARSFQGLDIQNGS 547
           +D QE +EE+N A  L +AT+KALQ+ D N  SL +GT +SGAE+F  RSFQGLDI N +
Sbjct: 422 FDLQEKIEEDNGALALANATDKALQLHDRNSTSLGKGTIKSGAEYFINRSFQGLDIHNDN 481

Query: 548 SQPEPYLIGRSGRASGYQDEK 565
           S PEPYLIGR+G+ASGY+ EK
Sbjct: 482 SMPEPYLIGRTGKASGYEHEK 502

BLAST of CmaCh06G016810 vs. TrEMBL
Match: A0A151SPP4_CAJCA (Diphthamide biosynthesis protein 2 OS=Cajanus cajan GN=KK1_003052 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 3.0e-183
Identity = 332/499 (66.53%), Postives = 395/499 (79.16%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALN--DPNQSDD 128
           M+ ES Y+I+ +A +IHT NFTRVALQFPD LLKDSTRVV+ALRKRL +L   D   S  
Sbjct: 1   MDFESNYDIAASAQFIHTHNFTRVALQFPDHLLKDSTRVVTALRKRLQSLKKCDATGSGH 60

Query: 129 ENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVS 188
           E  V LF+MADT YGSCCVDEVGA+H NADCVIHYGHTC SPTTTLPAFFVFGK  I V+
Sbjct: 61  ETDVGLFVMADTAYGSCCVDEVGASHINADCVIHYGHTCFSPTTTLPAFFVFGKASICVA 120

Query: 189 TCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSS 248
            C +++S Y+ +N KP++VLFGLEYAHSM  I++ LL+S  +    ++ EVHFA+V  S 
Sbjct: 121 DCVESMSKYALANSKPIMVLFGLEYAHSMQQIKEALLESSMSCRFDAKPEVHFANVPSSV 180

Query: 249 LDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSEN 308
           + PS   + +    EQ + A        +GT   IG L W LP  + M+D  +FWIG ++
Sbjct: 181 MFPSKDIKKINGLQEQASGA--------SGTTCSIGGLTWKLPEGQSMDDYLLFWIGLDD 240

Query: 309 SAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLG 368
           SAFANVVLTFN CEIVRYDA E  +VTD+ QQRRILKRRYYLVE+AKDA IVGILVGTLG
Sbjct: 241 SAFANVVLTFNACEIVRYDANEKQMVTDLFQQRRILKRRYYLVERAKDANIVGILVGTLG 300

Query: 369 LAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEY 428
           +AGYLHII+QM ELITGAGKKAYTLVMGRPNPAKLANFPEC VF+YVSCAQTAL+DSKEY
Sbjct: 301 VAGYLHIINQMMELITGAGKKAYTLVMGRPNPAKLANFPECDVFLYVSCAQTALLDSKEY 360

Query: 429 LAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVEDYD 488
           LAPVITPFEA +AF RGSQWTGAYVMEF+DLIN     E    +EAR+SFL+G YVED++
Sbjct: 361 LAPVITPFEAMIAFNRGSQWTGAYVMEFRDLINLP-EMEVIDQEEARFSFLKGGYVEDFE 420

Query: 489 SQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQP 548
           +QENVEEE E  +LV+ATEKALQ+R+N N+L++G ARSGAEF A RS+QGL++ + ++ P
Sbjct: 421 NQENVEEEREVLSLVNATEKALQLRNNSNALMKGNARSGAEFLANRSYQGLNMPSENNSP 480

Query: 549 EPYLIGRSGRASGYQDEKN 566
           EPYLIGR GRASGY+DEKN
Sbjct: 481 EPYLIGRRGRASGYEDEKN 490

BLAST of CmaCh06G016810 vs. TrEMBL
Match: D7T2D0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0094g00370 PE=3 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 5.2e-183
Identity = 335/507 (66.07%), Postives = 403/507 (79.49%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDEN 128
           MELES YEI+ TAD+I +RNFTRVALQFPDELLKD+TRVV ALR RL + ++   S D  
Sbjct: 1   MELESNYEIALTADFIRSRNFTRVALQFPDELLKDATRVVKALRDRLESPSESG-SGDGK 60

Query: 129 KVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTC 188
           +V LF+MADTTYGSCCVDEVGA+H NADCV+HYGHTCLSPT+ LPAFFVFGK PI +S C
Sbjct: 61  EVGLFVMADTTYGSCCVDEVGASHINADCVVHYGHTCLSPTSNLPAFFVFGKAPISISNC 120

Query: 189 AKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPL-LDSFQTSSLTSELEVHFADVKCSSL 248
           A+ LS Y+ + GK ++VLFGLEY+H+++ I++ L L+S + S  TS LE HFA+V CS +
Sbjct: 121 AENLSTYALTQGKHIMVLFGLEYSHAIEMIKEALMLESSKLSGSTSNLEFHFAEVMCSVM 180

Query: 249 DPS----SHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIG 308
           +PS    + N ++   G    N     F    GTR+ IG L W+LP   RMED  + WIG
Sbjct: 181 NPSKDCTTSNGHLGSVGSFTANE---GFGKATGTRYSIGGLAWNLPEGHRMEDYLLVWIG 240

Query: 309 SENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVG 368
           ++NSAFANVVLTFNGC+IVRYDA ++ LVTD+SQQRRILKRRYYLVEKAKD+ I+G+LVG
Sbjct: 241 ADNSAFANVVLTFNGCDIVRYDAVDNRLVTDLSQQRRILKRRYYLVEKAKDSNIIGLLVG 300

Query: 369 TLGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDS 428
           TLG+AGYLH+I QMKELI  AGKKAYTLVMGRPNPAKLANFPEC VFIYVSCAQTAL+DS
Sbjct: 301 TLGVAGYLHMIRQMKELIAAAGKKAYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDS 360

Query: 429 KEYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVE 488
           KE+LAPVITPFEA LAF RGSQWTGAYVMEF+DLI+ S  +  N+S+EAR+SFLQG YVE
Sbjct: 361 KEFLAPVITPFEAMLAFNRGSQWTGAYVMEFRDLISSSPVEVRNQSEEARFSFLQGGYVE 420

Query: 489 DYDSQEN---VEEENEACTLVSATEKALQIRDNR-NSLIEGTARSGAEFFAARSFQGLDI 548
           D+D +E     E+E+ A  L  ATEKALQ+RD   NSLI+GTA+SG EFFAARS+ GL++
Sbjct: 421 DFDLKETEDAEEDEDGALALAYATEKALQVRDKHPNSLIKGTAKSGGEFFAARSYHGLEM 480

Query: 549 QNGSSQPEPYLIGRSGRASGYQDEKNS 567
            +  S PEPYLIGR G+ASGY+ EK +
Sbjct: 481 HSNDSSPEPYLIGRVGKASGYEGEKGN 503

BLAST of CmaCh06G016810 vs. TAIR10
Match: AT3G59630.1 (AT3G59630.1 diphthamide synthesis DPH2 family protein)

HSP 1 Score: 563.9 bits (1452), Expect = 1.1e-160
Identity = 296/505 (58.61%), Postives = 379/505 (75.05%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDEN 128
           +E ES YEI+RTA++I +++FTR+ALQFPDELLKDST+VVSAL+ +   L D        
Sbjct: 3   LEFESKYEINRTAEFIISKSFTRIALQFPDELLKDSTKVVSALKSKTRLLTD-------R 62

Query: 129 KVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTC 188
           +VR F+MADTTYGSCC+DEVGA H +++CV+HYG TCLSPT+ LPAFFVFGK  I+VS+C
Sbjct: 63  EVRFFVMADTTYGSCCIDEVGALHIDSECVVHYGQTCLSPTSVLPAFFVFGKASINVSSC 122

Query: 189 AKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLD 248
            K L  Y+S + KP+++L+GLEYAH +  IR+ L      S   S+L V  A+V CS + 
Sbjct: 123 VKHLIDYASKSDKPIMILYGLEYAHVIPQIREEL----GLSKTDSQLSV--ANVLCSFIS 182

Query: 249 PSSHNENVARGGEQVTNAESGSFENIAGTR-HHIGSLFWDLPRERRMEDCSIFWIGSENS 308
           PS             +  ES S ++++ +R + +G L WDLP   ++ED  +FWIGS++S
Sbjct: 183 PSKDPRESMEHPRPYS--ESDSSDSLSSSRSYRLGGLTWDLPEGSKIEDYLLFWIGSDSS 242

Query: 309 AFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLGL 368
           AFANVVLTFNGC+IVRYDA+E  LVT+  QQRRILKRRYYLVEKAKDA I+GILVGTLG+
Sbjct: 243 AFANVVLTFNGCDIVRYDAEEDSLVTEFYQQRRILKRRYYLVEKAKDANIIGILVGTLGV 302

Query: 369 AGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEYL 428
           AGYLH+IH M+ LI+ AGKK+Y L MGRPNPAKLANFPEC VFIY+SCAQTAL+DSKE++
Sbjct: 303 AGYLHMIHHMQALISAAGKKSYILAMGRPNPAKLANFPECDVFIYISCAQTALLDSKEFM 362

Query: 429 APVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNR---SDEARYSFLQGRYVED 488
           +PVITPFEA LAF+RGS+WTGAY+M FQD+IN S+  E      S+E R+SF QG YVED
Sbjct: 363 SPVITPFEANLAFSRGSEWTGAYLMHFQDVIN-SVKSESEAHIGSEEPRFSFFQGGYVED 422

Query: 489 Y---DSQENVEEE-NEACTLVSATEKALQIRDN-RNSLIEGT-ARSGAEFFAARSFQGLD 548
           +   D  +N EE+  E  TLV A EKALQ+R N  NSL + T A+SG E+F  R ++GL+
Sbjct: 423 HKTNDQAKNGEEDTGETMTLVQAAEKALQLRGNDHNSLTKQTAAKSGPEYFLNRVYRGLE 482

Query: 549 IQNGSSQPEPYLIGRSGRASGYQDE 564
           I + ++ PEPY++GRSG+ASGY+ E
Sbjct: 483 INSENTLPEPYIVGRSGKASGYKHE 491

BLAST of CmaCh06G016810 vs. TAIR10
Match: AT5G62030.1 (AT5G62030.1 diphthamide synthesis DPH2 family protein)

HSP 1 Score: 74.3 bits (181), Expect = 2.7e-13
Identity = 43/151 (28.48%), Postives = 75/151 (49.67%), Query Frame = 1

Query: 74  YYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDENKVRLF 133
           ++E+ +    I T N  R+ALQ P+ LL  +  +         A +             F
Sbjct: 48  HFEVHKCVWRIKTSNAKRIALQLPEGLLMYALTLSDIFTSFAGASH------------CF 107

Query: 134 IMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSP--TTTLPAFFVFGKDPIDVSTCAKT 193
           ++ D TYG+CCVD+  A    AD +IHYGH+CL P  +T +P  +VF +  IDV     T
Sbjct: 108 VLGDVTYGACCVDDFSACALGADLLIHYGHSCLVPIDSTKIPCLYVFVEIQIDVKCLLNT 167

Query: 194 LSHYSSSNGKPVLVLFGLEYAHSMDDIRQPL 223
           +    +S+ K +++   +++  ++  ++  L
Sbjct: 168 IHLNLASDVKNIILAGTIQFTSAIRAVKPEL 186

BLAST of CmaCh06G016810 vs. NCBI nr
Match: gi|449468836|ref|XP_004152127.1| (PREDICTED: diphthamide biosynthesis protein 2 [Cucumis sativus])

HSP 1 Score: 872.1 bits (2252), Expect = 5.4e-250
Identity = 435/499 (87.17%), Postives = 464/499 (92.99%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALN--DPNQSDD 128
           ME ESYYEISRTAD+IHTRNFTRVALQFPDELLKDSTRVV AL+ RL  L+  D N SD 
Sbjct: 1   MEFESYYEISRTADFIHTRNFTRVALQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDS 60

Query: 129 ENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVS 188
           +++VRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPA FVFGKD IDV 
Sbjct: 61  KSEVRLFVMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPALFVFGKDSIDVP 120

Query: 189 TCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSS 248
           TCAKTLS YS S+GKPVLVLFGLEYAHSM DI+Q LLDSFQTSSL SELEVHFADVKCSS
Sbjct: 121 TCAKTLSQYSLSSGKPVLVLFGLEYAHSMLDIKQRLLDSFQTSSLGSELEVHFADVKCSS 180

Query: 249 LDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSEN 308
           LDPS H+ENV +GGEQ +NAESGS E+IAG RHHIG LFW+LP+ERR++DCS+FWIGSEN
Sbjct: 181 LDPSPHHENVVKGGEQASNAESGSSESIAGARHHIGGLFWELPKERRVKDCSLFWIGSEN 240

Query: 309 SAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLG 368
           SAFANVVLTFNGCEIVRYDAKES LVTDVS+QRRILKRRYYLVEKAKDAAIVGILVGTLG
Sbjct: 241 SAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYLVEKAKDAAIVGILVGTLG 300

Query: 369 LAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEY 428
           LAGYLHIIHQMKELITGAGKKAYTLVMG+PNPAKLANFPECGVFIYVSCAQTALMDSKEY
Sbjct: 301 LAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECGVFIYVSCAQTALMDSKEY 360

Query: 429 LAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVEDYD 488
           LAPVITPFEATLAF+RGSQWTGAYVMEFQDLI+FS P+E NRSDEARYSFLQG YVED+D
Sbjct: 361 LAPVITPFEATLAFSRGSQWTGAYVMEFQDLIDFSTPEEANRSDEARYSFLQGGYVEDWD 420

Query: 489 SQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQP 548
           SQEN EEEN A  LV+ATEKALQ+RDNRNSLIEGTARSGAEFFA RSFQGLDI NGS +P
Sbjct: 421 SQENTEEENGAHALVTATEKALQLRDNRNSLIEGTARSGAEFFATRSFQGLDINNGSLEP 480

Query: 549 EPYLIGRSGRASGYQDEKN 566
           EPY+IGRSG+ASGYQDEKN
Sbjct: 481 EPYVIGRSGKASGYQDEKN 499

BLAST of CmaCh06G016810 vs. NCBI nr
Match: gi|659108140|ref|XP_008454038.1| (PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Cucumis melo])

HSP 1 Score: 868.6 bits (2243), Expect = 5.9e-249
Identity = 433/499 (86.77%), Postives = 462/499 (92.59%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALN--DPNQSDD 128
           ME ESYYEISRTAD+IHTRNFTRVALQFPDELLKDSTRVV AL+ RL  L+  D N SD 
Sbjct: 1   MEFESYYEISRTADFIHTRNFTRVALQFPDELLKDSTRVVRALKDRLRVLDESDTNHSDS 60

Query: 129 ENKVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVS 188
           +N+VRLF+MADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPA FVFGKD IDV+
Sbjct: 61  KNEVRLFVMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPALFVFGKDSIDVT 120

Query: 189 TCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSS 248
           TCAKTLS YS S+GKPVLVLFGLEYAHSM DI+Q LLDSFQTSSL SELEVHFADVKCSS
Sbjct: 121 TCAKTLSQYSLSSGKPVLVLFGLEYAHSMFDIKQRLLDSFQTSSLGSELEVHFADVKCSS 180

Query: 249 LDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGSEN 308
           LDPS + ENV +GGEQ +NAESGS ENIAG RHHIG LFW+LP+ERRMEDCS+FWIGS+N
Sbjct: 181 LDPSPYQENVVKGGEQASNAESGSSENIAGARHHIGGLFWELPKERRMEDCSLFWIGSDN 240

Query: 309 SAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGTLG 368
           SAFANVVLTFNGCEIVRYDAKES LVTDVS+QRRILKRRYYLVEKAKDAAIVGILVGTLG
Sbjct: 241 SAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYLVEKAKDAAIVGILVGTLG 300

Query: 369 LAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSKEY 428
           LAGYLHIIHQMKELITGAGKKAYTLVMG+PNPAKLANFPECGVFIYVSCAQTALMDSKEY
Sbjct: 301 LAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECGVFIYVSCAQTALMDSKEY 360

Query: 429 LAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVEDYD 488
           LAPVITPFEATLAF+RGSQWTG YVMEFQDLI+ S PKE N+SDEARYSFLQG YVED+D
Sbjct: 361 LAPVITPFEATLAFSRGSQWTGVYVMEFQDLIDCSTPKEANQSDEARYSFLQGGYVEDWD 420

Query: 489 SQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEFFAARSFQGLDIQNGSSQP 548
           SQEN EEEN A  LV+AT+KALQ+RDNRN LIEGTARSGAEFFA+RSFQGLDI NGS +P
Sbjct: 421 SQENTEEENGAHALVTATQKALQLRDNRNLLIEGTARSGAEFFASRSFQGLDINNGSFEP 480

Query: 549 EPYLIGRSGRASGYQDEKN 566
           EPY+IGRSG+ASGYQDEKN
Sbjct: 481 EPYVIGRSGKASGYQDEKN 499

BLAST of CmaCh06G016810 vs. NCBI nr
Match: gi|659108142|ref|XP_008454039.1| (PREDICTED: diphthamide biosynthesis protein 2 isoform X2 [Cucumis melo])

HSP 1 Score: 695.7 bits (1794), Expect = 6.9e-197
Identity = 347/397 (87.41%), Postives = 370/397 (93.20%), Query Frame = 1

Query: 169 TTTLPAFFVFGKDPIDVSTCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQT 228
           TTTLPA FVFGKD IDV+TCAKTLS YS S+GKPVLVLFGLEYAHSM DI+Q LLDSFQT
Sbjct: 47  TTTLPALFVFGKDSIDVTTCAKTLSQYSLSSGKPVLVLFGLEYAHSMFDIKQRLLDSFQT 106

Query: 229 SSLTSELEVHFADVKCSSLDPSSHNENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDL 288
           SSL SELEVHFADVKCSSLDPS + ENV +GGEQ +NAESGS ENIAG RHHIG LFW+L
Sbjct: 107 SSLGSELEVHFADVKCSSLDPSPYQENVVKGGEQASNAESGSSENIAGARHHIGGLFWEL 166

Query: 289 PRERRMEDCSIFWIGSENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYL 348
           P+ERRMEDCS+FWIGS+NSAFANVVLTFNGCEIVRYDAKES LVTDVS+QRRILKRRYYL
Sbjct: 167 PKERRMEDCSLFWIGSDNSAFANVVLTFNGCEIVRYDAKESWLVTDVSKQRRILKRRYYL 226

Query: 349 VEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECG 408
           VEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMG+PNPAKLANFPECG
Sbjct: 227 VEKAKDAAIVGILVGTLGLAGYLHIIHQMKELITGAGKKAYTLVMGKPNPAKLANFPECG 286

Query: 409 VFIYVSCAQTALMDSKEYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNR 468
           VFIYVSCAQTALMDSKEYLAPVITPFEATLAF+RGSQWTG YVMEFQDLI+ S PKE N+
Sbjct: 287 VFIYVSCAQTALMDSKEYLAPVITPFEATLAFSRGSQWTGVYVMEFQDLIDCSTPKEANQ 346

Query: 469 SDEARYSFLQGRYVEDYDSQENVEEENEACTLVSATEKALQIRDNRNSLIEGTARSGAEF 528
           SDEARYSFLQG YVED+DSQEN EEEN A  LV+AT+KALQ+RDNRN LIEGTARSGAEF
Sbjct: 347 SDEARYSFLQGGYVEDWDSQENTEEENGAHALVTATQKALQLRDNRNLLIEGTARSGAEF 406

Query: 529 FAARSFQGLDIQNGSSQPEPYLIGRSGRASGYQDEKN 566
           FA+RSFQGLDI NGS +PEPY+IGRSG+ASGYQDEKN
Sbjct: 407 FASRSFQGLDINNGSFEPEPYVIGRSGKASGYQDEKN 443

BLAST of CmaCh06G016810 vs. NCBI nr
Match: gi|255579442|ref|XP_002530564.1| (PREDICTED: diphthamide biosynthesis protein 2 [Ricinus communis])

HSP 1 Score: 660.2 bits (1702), Expect = 3.2e-186
Identity = 336/501 (67.07%), Postives = 402/501 (80.24%), Query Frame = 1

Query: 69  MELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDPNQSDDEN 128
           M+ E  YEI  T D+IH+RNFTRVALQFPDELLKDS RVVSALR +L  L +     D  
Sbjct: 1   MDFEENYEIDLTTDFIHSRNFTRVALQFPDELLKDSVRVVSALRNKLELLEE--NGGDRK 60

Query: 129 KVRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDVSTC 188
           ++RLF+MADT YGSCCVDEVGA+HANADCVIHYGHTCLSPT+ LPAFFVFGK  I+VS+C
Sbjct: 61  EIRLFVMADTAYGSCCVDEVGASHANADCVIHYGHTCLSPTSNLPAFFVFGKASINVSSC 120

Query: 189 AKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCSSLD 248
           A++LS Y+ S+ KP++VL+GLEYAH++  IRQ L+ +    S     E+HFADV CS+++
Sbjct: 121 AQSLSDYAFSSTKPIMVLYGLEYAHAIPHIRQELIAAASMQSRPRHSELHFADVMCSAIN 180

Query: 249 PSSH----NENVARGGEQVTNAESGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGS 308
           PS++    +E     G+ V   + G     AG RH IG L WDL R +RMED  + WIGS
Sbjct: 181 PSNNQKKSDEPARLIGDCVATNDVGE---AAGCRHKIGGLIWDLSRGQRMEDYLLVWIGS 240

Query: 309 ENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGT 368
            NSAFANVVLTFNGCEIVRYDAKES LVTD+SQQRRILKRRYYLVEKAKDA IVGILVGT
Sbjct: 241 GNSAFANVVLTFNGCEIVRYDAKESRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGT 300

Query: 369 LGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSK 428
           LG+AGYL +IHQMK+LIT AGKKAYTLVMGRPNPAKLANFPEC +FIYVSCAQTAL+DSK
Sbjct: 301 LGVAGYLDMIHQMKDLITSAGKKAYTLVMGRPNPAKLANFPECDIFIYVSCAQTALLDSK 360

Query: 429 EYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVED 488
           E+LAPVITPFEA +AF RGSQWTGAYVMEF+DLI+ S  +   +  EAR+SF+QGRYVED
Sbjct: 361 EFLAPVITPFEAMIAFNRGSQWTGAYVMEFRDLIDSSPVEAMIKPAEARFSFIQGRYVED 420

Query: 489 YDSQENVEEENEACTLVSATEKALQIRD-NRNSLIEGTARSGAEFFAARSFQGLDIQNGS 548
           +D QEN+EE+NE   L +ATEKALQ+ D N  SLI+GT +SGAE+FA RS+QGL++   +
Sbjct: 421 FDLQENIEEDNEGLALANATEKALQLHDRNSTSLIKGTIKSGAEYFANRSYQGLEMHGDN 480

Query: 549 SQPEPYLIGRSGRASGYQDEK 565
           S+P  +LIGR+G+ASGY++EK
Sbjct: 481 SKPGAFLIGRTGKASGYENEK 496

BLAST of CmaCh06G016810 vs. NCBI nr
Match: gi|802554777|ref|XP_012065078.1| (PREDICTED: diphthamide biosynthesis protein 2 [Jatropha curcas])

HSP 1 Score: 650.6 bits (1677), Expect = 2.5e-183
Identity = 331/501 (66.07%), Postives = 399/501 (79.64%), Query Frame = 1

Query: 68  AMELESYYEISRTADYIHTRNFTRVALQFPDELLKDSTRVVSALRKRLCALNDP-NQSDD 127
           AM+ E  YEI+RTAD+IH+RNFTRVALQFPDELLKDS RVVSALR +L +L     +   
Sbjct: 2   AMDFEENYEINRTADFIHSRNFTRVALQFPDELLKDSVRVVSALRDKLQSLRASIAEKIG 61

Query: 128 ENK-VRLFIMADTTYGSCCVDEVGAAHANADCVIHYGHTCLSPTTTLPAFFVFGKDPIDV 187
           ENK  RLF+MADTTYGSCCVDEVGA+H +ADCVIHYGHTCLSPT+TLPAFFVFGK  I +
Sbjct: 62  ENKEARLFVMADTTYGSCCVDEVGASHIDADCVIHYGHTCLSPTSTLPAFFVFGKASISI 121

Query: 188 STCAKTLSHYSSSNGKPVLVLFGLEYAHSMDDIRQPLLDSFQTSSLTSELEVHFADVKCS 247
            +CA++LS Y+S+N KP++V++GLEYAH++  I++  + +    S     +  FADV CS
Sbjct: 122 HSCAQSLSDYASTNSKPIVVIYGLEYAHAIPHIKEKFMAASSIESGPKHSQSLFADVMCS 181

Query: 248 SLDPSSHNENVARGGEQVTNAE-SGSFENIAGTRHHIGSLFWDLPRERRMEDCSIFWIGS 307
            ++P   ++N     + + +   S   +   G++H IG L W+LP  RRMED  + WIGS
Sbjct: 182 VINPFDSHKNSDGLPKLLGDYNASNDVDAAVGSKHKIGGLIWELPNGRRMEDYLLVWIGS 241

Query: 308 ENSAFANVVLTFNGCEIVRYDAKESCLVTDVSQQRRILKRRYYLVEKAKDAAIVGILVGT 367
           ++SAFANVVLTFNGCEIVRYDAKE+ LVTD+SQQRRILKRRYYLVEKAKDA IVGILVGT
Sbjct: 242 DSSAFANVVLTFNGCEIVRYDAKENRLVTDLSQQRRILKRRYYLVEKAKDANIVGILVGT 301

Query: 368 LGLAGYLHIIHQMKELITGAGKKAYTLVMGRPNPAKLANFPECGVFIYVSCAQTALMDSK 427
           LG+AGYL +IHQMK+LIT AGKK YTLVMGRPNPAKLANFPEC VFIYVSCAQTAL+DSK
Sbjct: 302 LGVAGYLDMIHQMKDLITNAGKKTYTLVMGRPNPAKLANFPECDVFIYVSCAQTALLDSK 361

Query: 428 EYLAPVITPFEATLAFTRGSQWTGAYVMEFQDLINFSIPKEGNRSDEARYSFLQGRYVED 487
           E+LAPVITPFEA +AF RGS WTG YVMEF+DLIN S  +  N S+EAR+SF+QGRYVED
Sbjct: 362 EFLAPVITPFEAMVAFNRGSLWTGEYVMEFRDLINSSPAEARNNSEEARFSFMQGRYVED 421

Query: 488 YDSQENVEEENEACTLVSATEKALQIRD-NRNSLIEGTARSGAEFFAARSFQGLDIQNGS 547
           +D QE +EE+N A  L +AT+KALQ+ D N  SL +GT +SGAE+F  RSFQGLDI N +
Sbjct: 422 FDLQEKIEEDNGALALANATDKALQLHDRNSTSLGKGTIKSGAEYFINRSFQGLDIHNDN 481

Query: 548 SQPEPYLIGRSGRASGYQDEK 565
           S PEPYLIGR+G+ASGY+ EK
Sbjct: 482 SMPEPYLIGRTGKASGYEHEK 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DPH2_DANRE7.1e-7733.78Diphthamide biosynthesis protein 2 OS=Danio rerio GN=dph2 PE=2 SV=1[more]
DPH2_CHICK8.2e-7332.93Diphthamide biosynthesis protein 2 OS=Gallus gallus GN=DPH2 PE=2 SV=1[more]
DPH2_NEMVE8.4e-7031.68Diphthamide biosynthesis protein 2 OS=Nematostella vectensis GN=dph2 PE=3 SV=1[more]
DPH2_SCHPO1.0e-6730.19Diphthamide biosynthesis protein 2 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
DPH2_XENLA1.3e-6232.54Diphthamide biosynthesis protein 2 OS=Xenopus laevis GN=dph2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KW05_CUCSA3.7e-25087.17Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011710 PE=3 SV=1[more]
B9SX45_RICCO2.2e-18667.07Diphteria toxin resistance protein 2, dph2, putative OS=Ricinus communis GN=RCOM... [more]
A0A067L745_JATCU1.8e-18366.07Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05728 PE=3 SV=1[more]
A0A151SPP4_CAJCA3.0e-18366.53Diphthamide biosynthesis protein 2 OS=Cajanus cajan GN=KK1_003052 PE=3 SV=1[more]
D7T2D0_VITVI5.2e-18366.07Putative uncharacterized protein OS=Vitis vinifera GN=VIT_05s0094g00370 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G59630.11.1e-16058.61 diphthamide synthesis DPH2 family protein[more]
AT5G62030.12.7e-1328.48 diphthamide synthesis DPH2 family protein[more]
Match NameE-valueIdentityDescription
gi|449468836|ref|XP_004152127.1|5.4e-25087.17PREDICTED: diphthamide biosynthesis protein 2 [Cucumis sativus][more]
gi|659108140|ref|XP_008454038.1|5.9e-24986.77PREDICTED: diphthamide biosynthesis protein 2 isoform X1 [Cucumis melo][more]
gi|659108142|ref|XP_008454039.1|6.9e-19787.41PREDICTED: diphthamide biosynthesis protein 2 isoform X2 [Cucumis melo][more]
gi|255579442|ref|XP_002530564.1|3.2e-18667.07PREDICTED: diphthamide biosynthesis protein 2 [Ricinus communis][more]
gi|802554777|ref|XP_012065078.1|2.5e-18366.07PREDICTED: diphthamide biosynthesis protein 2 [Jatropha curcas][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR010014DHP2_euk
IPR016435DPH1/DPH2
Vocabulary: Biological Process
TermDefinition
GO:0017183peptidyl-diphthamide biosynthetic process from peptidyl-histidine
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017183 peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process GO:0015824 proline transport
cellular_component GO:0005575 cellular_component
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function
molecular_function GO:0016740 transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G016810.1CmaCh06G016810.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010014Diphthamide synthesis DHP2, eukaryoticPANTHERPTHR10762:SF2DIPHTHAMIDE BIOSYNTHESIS PROTEIN 2coord: 69..566
score: 1.8E
IPR010014Diphthamide synthesis DHP2, eukaryoticTIGRFAMsTIGR00272TIGR00272coord: 64..561
score: 7.5
IPR016435Diphthamide synthesis DPH1/DPH2PANTHERPTHR10762DIPHTHAMIDE BIOSYNTHESIS PROTEINcoord: 69..566
score: 1.8E
IPR016435Diphthamide synthesis DPH1/DPH2PFAMPF01866Diphthamide_syncoord: 96..447
score: 3.4
IPR016435Diphthamide synthesis DPH1/DPH2TIGRFAMsTIGR00322TIGR00322coord: 74..440
score: 5.9E