CmaCh06G016010 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh06G016010
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionMLP-like protein 28
LocationCma_Chr06 : 9974773 .. 9978449 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTTTTTTTGTCCAAAATCATATAATAAAATATTAGTATCATGAAAAGTTATGGTATTCAATATTATAATATCATGTGAGAGTTAAACCTTCTCTATTTAAGTGTACGAGGAGACAAGCTTGATCATCCATCGTCTTATTTTTTCTCGTCTCAAGTTTCTTGTTTTTTTTAAATCCGACCATCATGAGTCTGTGTGGAAAATTAGAGACTGATGTAAAAATCAAGGTCCCAGCTTCAAAGTTCCATGAAATGTTTCATCGAAAGCCTCACCATATCTCCAAAGCGTGTGGAGACAAAATCCAAGGTTGTCAGCTCCAAGAAGGCGAATGGGGCAAAGTTGGCTCGATCATTTACTGGAACTATTGTCATGGTTTGTTCTTGTTATTGTGTTTGTTTGTGTTTTTTTAAGGATGTTTTCGAGTTTTCCTATGTTTCTTTAATGATGTTTGTTAAACTCGTAGGTGGGAAGGCTTCAGTAGCCAAGGAGGTAGTTGAAGCAGTTGATGAGGAGAACAATAAGATATGTTTCAAAGTCGTAGAAGGAGACCTTTTGAAGGATTATAAGAGCTTTAGAATTACAATTCAGTGTATTCCAAAGGATAAGGGAAGCGTGGTTCATTGGATTTTCGAGTATGAAAAGCGACACGAGAAAATTCCTGATTCACATAGTTTACTTCATTTGTGTGTTGCTATCTCGAAAGACATCGAAGCTCATCTCCTCGGTAATGAAAAACCATGTGGCGCGTAAGTCGGAGTACTTAAGAATAAGATATCTGGACACGTTTCTACGTTGATATGTTTAAATAAGATCATCTGTTTGTGTGTGTAATATGATCGAGACATGTTTGGAATCATGTTTGAAATGGGAATAAAGTTTGTAAGAATATGTGTGTACTAAAAAATAATAAATGTACGTTATAATAAGGATTATTGTGTTTCTCGGGCTTGACATTGATCTAGTCTTCTCGGCACTCTACGACTCTCCATGTTATTTTATTAAAAAGAATAAAAAATTAGAATTCTGATACTACTTGTCACAATCGTACTCTAGTAACAGCTGGACAACAACTGTGCGGCACTTGTTATAACCTATCAAATAAGTCATTCGTGAGAAATTCAGACTTTGCGGATTACATCAACGTATGTTCGAGCCTCGTGACACGTTTGAGAGAAATGATTTAGAAAACATGAGCGAGAAAATACGTTGAAAATAATTTGAAAGAAAGCAGTGTTATAAGCTAAAAGCAATCAGACAACAACGACTTTGATAGCATAAAAATCCATTCAAATTACAATAATAATTGAGGAGCTAATTAATATCGATTTAATTTTTACCAATAATAGAATCATATTATTGATATCCGTCGTCGATTATATTCTTACTTAATTGAACGACTATCAGAGATTTATTTAAAACAAAAATAATTCATAATTGGCGGAAGACTGAGCGCTATGTCCCGGGCATTCGCCAGATACCCCTTAACCTGGGTCTACCAACCGACTTAAAGCAATAGGATGTTTGTTCTAACAGGTCTTTCTTCTTCTTTCCCAAATCGATTGTTTGCTGTGGCGGGTCGCAAGATGTGGCTTTTTCCTTCTGTCTGTTCGGGGTTGTCTCTTCTTCCTCTTCCTTCCTTTTGTTAACACAATTGATAACGAAAATACTGTAAAATTTTGACAAAATCAAAGTATTATAACAAAAATGGGGACCACGGAACGAGATATAACCCCTAACACAGGACTAACGCTTAAGGATAACCAATAGTCGTTAGTCACTTGAAAATGAAAAGTAGTACTTGTCTTCCAGAAGTGTGTTTTGTGAATGTTTCATTCTACTTCGATTACTAGGTTCGGTGTGCTTTTGTAACAGCTCAAGTTCATTGCTATCAGATATTGTCTGTTTTGGTCCGTTACGTATCGTCGTCAGCCTCATGATTTTAAAACTCGTCTATTAGGGAGAGGTTTCCACTCTTATAAGGAATGTTCCGCCAACTGATACGGGATCTCCCAGCTTCGATCGGTGGTCAAAACCAACGCTCCAAATTATTATTTTTTTTCACGTAATTCCTACTCACATAACCAAATAGGATGACACTACAATAATTCATATTTTGATCAAAATCGTAGCCTAATGGGATCCATGGCTATGCAACCAAACCCTCTCTATAAATGAGACATGTTTAGCAATGCATTACTCCAAGAACCAAGCACACCATGGACCTCTTTGGAAAGGTGGAGGCTGATGTAGAAATCACTGCTTCAGCTTCTAAGTTTCATCAAATATTTCACCACAGGCCTCATCATATTTCCAATTTCTCTAGGGGCATAATACATGGCGTTGATCTACTCGAAGGCGAATGGGGCAAAGTCGGGTCCATCGTCATTTGGAGATATTCACATGGTTTATCATTTTTTCGATGTTTACTTTCTTTACACTTTTCGGTAGGACTGTGCATGTGACTCGAGAATCTGACGAACTCAAACTACTCAACCTAAACGATAAAAGTTGGGTTGGGTTGAAATTTTTTAAAATCGAAAATTCAGGTCGGTTCATGGGTTGATGTTTTTTTTTTTTTGTTAGGTCAGCCTGACCACCCAACCAACTCGAAAATATGTTACAACTCATCATATGACCCGAGAATCCAACAAACTCAGACTACCCAATTTAAACGATAAAAGTTGGGTTTGGTTGAAATTTTGAAAATCAAAAATTCGGATCAGTTCGTAGGTTGATATTTTTTTTTTTGTTAGGTGAGTCTGACCAAACAACTAACCCAACCAACTCAAAAGTATGTTACAACTCAATCATATGACCCGAGAATCCAACAAATTCAGACTACCCAACCTAAATGATAAAAGTTGGGTTGGGTTAAAATTTTGAAAATCGAAAATTTGGGTTGGTTCGTGGGTTGATATCTTTTTTGTTAGGTTAGCCTGACCAACCAACCAACCCAACCAGCTCAAAAATATGTTACAACTCATCATATGACCCGAGAATCCAACAAACTCAGACTACCCAACCTAAACGGTAAAAGTTGGGTTGGGTTGAAATTTTCGAAAATCGAAAATTCAGGTCAGTTTGTGGGTTGGTACTTTTTTTGTTAGGTCAACCTGACCAACTAACCCAACCAACTTGAAAATATGCTACAACCCAACCCTACATTACACTACTTTTAAGACTATTATGATATTAAGTATGTTTCAAAATGATGTTAATGGTACGATTCAATTCGACCCAACTCGAAAATAAAAGGTTGAGTTGAATTGGGTTGTGAAATGTATATGGGTGGCTCGGGTTACCAACCCACCCAACCTGAAATTTAGAGTTAGTTGACGAGATTTCTTCAACCCAGTTCGTGTACGGAACAGTAACATGAGTTTAATTTGTAGATGGGAAGGTTGTGGCAAGCAAAGAGATAATTGAAGCCGTTGATGAAGAGAACAACTCTATCACTTTCACAGCCATTGAAGGATGCCTTTTGCAGCAATATAAGAGCTTCAAGTTGAAGCTTCAATGTATCCCAAAGGAGAAGGGAAGCGTTGTTCATTGGACTTTAGAATATGAAAAATTGCACGATGGGATTCCAGACTCCCATGGCTTGCTTCAATTATGTGTTGCTATGTCCAAAGACATTGATGCTTACCTTATGGAAGACAATGAAGTGGCATGA

mRNA sequence

TTTTTTTTTTTTTTTGTCCAAAATCATATAATAAAATATTAGTATCATGAAAAGTTATGGTATTCAATATTATAATATCATGTGAGAGTTAAACCTTCTCTATTTAAGTGTACGAGGAGACAAGCTTGATCATCCATCGTCTTATTTTTTCTCGTCTCAAGTTTCTTGTTTTTTTTAAATCCGACCATCATGAGTCTGTGTGGAAAATTAGAGACTGATGTAAAAATCAAGGTCCCAGCTTCAAAGTTCCATGAAATGTTTCATCGAAAGCCTCACCATATCTCCAAAGCGTGTGGAGACAAAATCCAAGGTTGTCAGCTCCAAGAAGGCGAATGGGGCAAAGTTGGCTCGATCATTTACTGGAACTATTGTCATGGTGGGAAGGCTTCAGTAGCCAAGGAGGTAGTTGAAGCAGTTGATGAGGAGAACAATAAGATATGTTTCAAAGTCGTAGAAGGAGACCTTTTGAAGGATTATAAGAGCTTTAGAATTACAATTCAGTGTATTCCAAAGGATAAGGGAAGCGTGGTTCATTGGATTTTCGAGTATGAAAAGCGACACGAGAAAATTCCTGATTCACATAGTTTACTTCATTTGTGTGTTGCTATCTCGAAAGACATCGAAGCTCATCTCCTCGCCGTTGATGAAGAGAACAACTCTATCACTTTCACAGCCATTGAAGGATGCCTTTTGCAGCAATATAAGAGCTTCAAGTTGAAGCTTCAATGTATCCCAAAGGAGAAGGGAAGCGTTGTTCATTGGACTTTAGAATATGAAAAATTGCACGATGGGATTCCAGACTCCCATGGCTTGCTTCAATTATGTGTTGCTATGTCCAAAGACATTGATGCTTACCTTATGGAAGACAATGAAGTGGCATGA

Coding sequence (CDS)

ATGAGTCTGTGTGGAAAATTAGAGACTGATGTAAAAATCAAGGTCCCAGCTTCAAAGTTCCATGAAATGTTTCATCGAAAGCCTCACCATATCTCCAAAGCGTGTGGAGACAAAATCCAAGGTTGTCAGCTCCAAGAAGGCGAATGGGGCAAAGTTGGCTCGATCATTTACTGGAACTATTGTCATGGTGGGAAGGCTTCAGTAGCCAAGGAGGTAGTTGAAGCAGTTGATGAGGAGAACAATAAGATATGTTTCAAAGTCGTAGAAGGAGACCTTTTGAAGGATTATAAGAGCTTTAGAATTACAATTCAGTGTATTCCAAAGGATAAGGGAAGCGTGGTTCATTGGATTTTCGAGTATGAAAAGCGACACGAGAAAATTCCTGATTCACATAGTTTACTTCATTTGTGTGTTGCTATCTCGAAAGACATCGAAGCTCATCTCCTCGCCGTTGATGAAGAGAACAACTCTATCACTTTCACAGCCATTGAAGGATGCCTTTTGCAGCAATATAAGAGCTTCAAGTTGAAGCTTCAATGTATCCCAAAGGAGAAGGGAAGCGTTGTTCATTGGACTTTAGAATATGAAAAATTGCACGATGGGATTCCAGACTCCCATGGCTTGCTTCAATTATGTGTTGCTATGTCCAAAGACATTGATGCTTACCTTATGGAAGACAATGAAGTGGCATGA

Protein sequence

MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYCHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEYEKRHEKIPDSHSLLHLCVAISKDIEAHLLAVDEENNSITFTAIEGCLLQQYKSFKLKLQCIPKEKGSVVHWTLEYEKLHDGIPDSHGLLQLCVAMSKDIDAYLMEDNEVA
Homology
[Close]
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match: MLP31_ARATH (MLP-like protein 31 OS=Arabidopsis thaliana GN=MLP31 PE=2 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 2.0e-49
Identity = 92/150 (61.33%), Postives = 112/150 (74.67%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SLCGKLETD++IK  A KFH MF  +PHH+SKA   KIQGC+L EG+WGKVGSI++WNY 
Sbjct: 19  SLCGKLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYV 78

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ E N I F+V+EGDLLK+YKSF ITIQ  PK    GSVVHW  E
Sbjct: 79  HDGEAKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVE 138

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLL 150
           YEK  +K+    + L  CV +SK+I+ HLL
Sbjct: 139 YEKIDDKVAHPETFLDFCVEVSKEIDEHLL 168

[Close]
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match: MLP28_ARATH (MLP-like protein 28 OS=Arabidopsis thaliana GN=MLP28 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.3e-48
Identity = 91/155 (58.71%), Postives = 113/155 (72.90%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLETDV+IK  A KFH MF  KPHH+SKA    IQGC L EG+WG VGSI++WNY 
Sbjct: 21  SLVGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYV 80

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ + N I F+V+EGDL+K+YKSF +TIQ  PK    GS+VHW  E
Sbjct: 81  HDGEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLE 140

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLAVDEE 155
           YEK  E++    +LL  CV +SK+I+ HLLA +EE
Sbjct: 141 YEKISEEVAHPETLLQFCVEVSKEIDEHLLAEEEE 175

[Close]
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match: MLP43_ARATH (MLP-like protein 43 OS=Arabidopsis thaliana GN=MLP43 PE=1 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.2e-46
Identity = 87/151 (57.62%), Postives = 107/151 (70.86%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLET+V+IK  A KFH MF  +PHH+SKA  DKI GC+L EG+WGKVGSI+ W Y 
Sbjct: 6   SLVGKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIVIWKYV 65

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H GK +V K  +EAVD E N I FKV+EGDL+ +YKSF  T+Q  PK  + GS+ HW  E
Sbjct: 66  HDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGESGSIAHWHLE 125

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           YEK  E++    +LL  CV ISK+I+ HLLA
Sbjct: 126 YEKISEEVAHPETLLQFCVEISKEIDEHLLA 156

[Close]
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match: MLP34_ARATH (MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.0e-46
Identity = 87/155 (56.13%), Postives = 112/155 (72.26%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLET+V+IK  A +FH MF  KPHH+SKA    IQ C L EG+WG VGSI++WNY 
Sbjct: 8   SLVGKLETEVEIKASAGQFHHMFAGKPHHVSKASPGNIQSCDLHEGDWGTVGSIVFWNYV 67

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ E N I F+V+EGDL+K+YKSF ITIQ  PK    GS+VHW  E
Sbjct: 68  HDGEAKVAKERIEAVEPEKNLITFRVIEGDLMKEYKSFLITIQVTPKHGGPGSIVHWHLE 127

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLAVDEE 155
           YEK  +++    +LL  CV +S++I+ HLL+ +EE
Sbjct: 128 YEKISDEVAHPETLLQFCVEVSQEIDEHLLSEEEE 162

[Close]
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match: KIRO_ACTDE (Kirola OS=Actinidia deliciosa PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 1.9e-28
Identity = 62/149 (41.61%), Postives = 92/149 (61.74%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           M L GK+   V+I      F+E+F  + + IS+     IQG  L EG WG VGS+I++ Y
Sbjct: 1   MDLSGKMVKQVEILSDGIVFYEIFRYRLYLISEMSPVNIQGVDLLEGNWGTVGSVIFFKY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
              GK   AK++VEA+DEE   + FK+VEGDL++ YK+F I +Q   K + + V W F Y
Sbjct: 61  TIDGKEKTAKDIVEAIDEETKSVTFKIVEGDLMELYKTFIIIVQVDTKGEHNSVTWTFHY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHLL 150
           EK  E + + ++L++ C+ I+KDIE + L
Sbjct: 121 EKLKEDVEEPNTLMNFCIEITKDIETYHL 149

[Close]
BLAST of CmaCh06G016010 vs. TrEMBL
Match: M4CUQ4_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 2.6e-56
Identity = 123/256 (48.05%), Postives = 155/256 (60.55%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           +L GKLETDV+IK PA KFH MF  +PHH+SKA    IQ C+L EG+WGKV         
Sbjct: 6   TLVGKLETDVEIKAPAGKFHHMFAGRPHHVSKATPGNIQNCELHEGDWGKV--------- 65

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
             G+A VAKE VEAV+ E N I F+VVEGDL+K+YKSF ITIQ  PK    GSVVHW  E
Sbjct: 66  -DGEAKVAKERVEAVEPEKNLITFRVVEGDLMKEYKSFVITIQVTPKHGGPGSVVHWHLE 125

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLL---------------------------AVD 181
           YEK  +++    +LL  CV ISK+I+ HLL                           AV+
Sbjct: 126 YEKISDEVAHPETLLQFCVEISKEIDEHLLNEEMERQRWLRRGSRRGEAKGAKERVEAVE 185

Query: 182 EENNSITFTAIEGCLLQQYKSFKLKLQCIPKE--KGSVVHWTLEYEKLHDGIPDSHGLLQ 227
            E N ITF  +EG L+++YKSF + +Q  PK    GSVVHW LEYEK+ D +     LLQ
Sbjct: 186 PEKNLITFRVVEGDLMKEYKSFVITIQVTPKHGGPGSVVHWYLEYEKISDEVAHPETLLQ 245

[Close]
BLAST of CmaCh06G016010 vs. TrEMBL
Match: Q9SXL8_CUCSA (Csf-2 protein OS=Cucumis sativus GN=Csf-2 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 4.4e-56
Identity = 102/150 (68.00%), Postives = 118/150 (78.67%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           MSLCGKLE DV I+  ASKFHEMFH+KPHHI     DKI G +LQEGEWG+VGSII W Y
Sbjct: 1   MSLCGKLEKDVPIRASASKFHEMFHKKPHHICNCSTDKIHGVELQEGEWGQVGSIICWKY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
            H GK  +AKE+VE VDEENN I FKV+EGDL + YK FR TI+CIPK+KGSV+HW+ EY
Sbjct: 61  FHEGKHKIAKEIVEHVDEENNSITFKVIEGDLTEHYKDFRFTIKCIPKEKGSVIHWVLEY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           EK H+KIPDSH+LL  CV +SKDI+  L A
Sbjct: 121 EKLHDKIPDSHTLLQFCVDVSKDIDKQLSA 150

[Close]
BLAST of CmaCh06G016010 vs. TrEMBL
Match: A0A0A0L9B6_CUCSA (Csf-2 protein OS=Cucumis sativus GN=Csa_3G172375 PE=4 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.2e-55
Identity = 101/151 (66.89%), Postives = 120/151 (79.47%), Query Frame = 1 

Query: 3   LCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYCH 62
           L GKLETDV IK  AS FHE+FH+KPHHIS A  DKI G  L EGEWG+VGSII W Y H
Sbjct: 4   LYGKLETDVPIKASASMFHEIFHKKPHHISNASTDKIHGVDLHEGEWGQVGSIICWKYFH 63

Query: 63  GGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEYEK 122
            GKA +AKE++E VDEENN I FKV+EGDL + YK FR+TIQCIPK+KGSV+HWI EYEK
Sbjct: 64  DGKARIAKEIIEHVDEENNSITFKVIEGDLTEHYKDFRLTIQCIPKEKGSVIHWILEYEK 123

Query: 123 RHEKIPDSHSLLHLCVAISKDIEAHLLAVDE 154
            H++IPDSH+LL  CV +S+DI++ L  +DE
Sbjct: 124 LHDQIPDSHTLLQFCVEVSQDIDSMLSDIDE 154

[Close]
BLAST of CmaCh06G016010 vs. TrEMBL
Match: A0A0A0L885_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171860 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 7.0e-54
Identity = 99/144 (68.75%), Postives = 116/144 (80.56%), Query Frame = 1 

Query: 6   KLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYCHGGK 65
           KLETDV I+ PA KFHEM HR PHH+S    DK+Q C+L EGEWGKVGSII+WNY H GK
Sbjct: 7   KLETDVVIEAPAWKFHEMLHRNPHHLSNIAADKVQSCELHEGEWGKVGSIIFWNYVHDGK 66

Query: 66  ASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEYEKRHE 125
           A VAK+V+EAVDEENN   +KV+EGDLL  YK+FR+TIQ IPKDKGSV+H+   YEK HE
Sbjct: 67  ACVAKDVIEAVDEENNSFTWKVIEGDLLDHYKNFRVTIQSIPKDKGSVIHYTLNYEKLHE 126

Query: 126 KIPDSHSLLHLCVAISKDIEAHLL 150
            I DSH+LL LCV+ISK I+AHL+
Sbjct: 127 DIEDSHTLLDLCVSISKAIDAHLM 150

[Close]
BLAST of CmaCh06G016010 vs. TrEMBL
Match: Q9AXU0_PRUPE (Major latex-like protein OS=Prunus persica GN=PRUPE_ppa012742mg PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 1.5e-48
Identity = 90/146 (61.64%), Postives = 110/146 (75.34%), Query Frame = 1 

Query: 5   GKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYCHGG 64
           GK+ETDV+IK PA+KFHE+F  +PHHIS    + IQGC L EGEWG VGS++YWNY H G
Sbjct: 6   GKVETDVEIKAPATKFHEVFTHRPHHISNVSSNNIQGCDLHEGEWGTVGSVVYWNYFHDG 65

Query: 65  KASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFEYEK 124
           KA VAKE++EA+D E N I FKV+EGDLL+ YKSF+ITI   PK   +GS+VHW  EYEK
Sbjct: 66  KAKVAKELIEAIDAEKNLITFKVIEGDLLEHYKSFKITIHATPKAHGQGSIVHWTMEYEK 125

Query: 125 RHEKIPDSHSLLHLCVAISKDIEAHL 149
            H  I D H+LL   V +SKD++AHL
Sbjct: 126 HHGDIEDPHTLLQFAVDVSKDVDAHL 151

[Close]
BLAST of CmaCh06G016010 vs. TAIR10
Match: AT1G70840.1 (AT1G70840.1 MLP-like protein 31)

HSP 1 Score: 197.2 bits (500), Expect = 1.1e-50
Identity = 92/150 (61.33%), Postives = 112/150 (74.67%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SLCGKLETD++IK  A KFH MF  +PHH+SKA   KIQGC+L EG+WGKVGSI++WNY 
Sbjct: 19  SLCGKLETDIEIKASAGKFHHMFAGRPHHVSKATPGKIQGCELHEGDWGKVGSIVFWNYV 78

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ E N I F+V+EGDLLK+YKSF ITIQ  PK    GSVVHW  E
Sbjct: 79  HDGEAKVAKERIEAVEPEKNLITFRVIEGDLLKEYKSFVITIQVTPKRGGPGSVVHWHVE 138

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLL 150
           YEK  +K+    + L  CV +SK+I+ HLL
Sbjct: 139 YEKIDDKVAHPETFLDFCVEVSKEIDEHLL 168

[Close]
BLAST of CmaCh06G016010 vs. TAIR10
Match: AT1G70830.1 (AT1G70830.1 MLP-like protein 28)

HSP 1 Score: 194.5 bits (493), Expect = 7.1e-50
Identity = 91/155 (58.71%), Postives = 113/155 (72.90%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLETDV+IK  A KFH MF  KPHH+SKA    IQGC L EG+WG VGSI++WNY 
Sbjct: 21  SLVGKLETDVEIKASADKFHHMFAGKPHHVSKASPGNIQGCDLHEGDWGTVGSIVFWNYV 80

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ + N I F+V+EGDL+K+YKSF +TIQ  PK    GS+VHW  E
Sbjct: 81  HDGEAKVAKERIEAVEPDKNLITFRVIEGDLMKEYKSFLLTIQVTPKPGGPGSIVHWHLE 140

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLAVDEE 155
           YEK  E++    +LL  CV +SK+I+ HLLA +EE
Sbjct: 141 YEKISEEVAHPETLLQFCVEVSKEIDEHLLAEEEE 175

[Close]
BLAST of CmaCh06G016010 vs. TAIR10
Match: AT1G70890.1 (AT1G70890.1 MLP-like protein 43)

HSP 1 Score: 188.0 bits (476), Expect = 6.7e-48
Identity = 87/151 (57.62%), Postives = 107/151 (70.86%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLET+V+IK  A KFH MF  +PHH+SKA  DKI GC+L EG+WGKVGSI+ W Y 
Sbjct: 6   SLVGKLETEVEIKASAKKFHHMFTERPHHVSKATPDKIHGCELHEGDWGKVGSIVIWKYV 65

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H GK +V K  +EAVD E N I FKV+EGDL+ +YKSF  T+Q  PK  + GS+ HW  E
Sbjct: 66  HDGKLTVGKNKIEAVDPEKNLITFKVLEGDLMNEYKSFAFTLQVTPKQGESGSIAHWHLE 125

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           YEK  E++    +LL  CV ISK+I+ HLLA
Sbjct: 126 YEKISEEVAHPETLLQFCVEISKEIDEHLLA 156

[Close]
BLAST of CmaCh06G016010 vs. TAIR10
Match: AT1G70850.1 (AT1G70850.1 MLP-like protein 34)

HSP 1 Score: 187.2 bits (474), Expect = 1.1e-47
Identity = 87/155 (56.13%), Postives = 112/155 (72.26%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLET+V+IK  A +FH MF  KPHH+SKA    IQ C L EG+WG VGSI++WNY 
Sbjct: 8   SLVGKLETEVEIKASAGQFHHMFAGKPHHVSKASPGNIQSCDLHEGDWGTVGSIVFWNYV 67

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPK--DKGSVVHWIFE 121
           H G+A VAKE +EAV+ E N I F+V+EGDL+K+YKSF ITIQ  PK    GS+VHW  E
Sbjct: 68  HDGEAKVAKERIEAVEPEKNLITFRVIEGDLMKEYKSFLITIQVTPKHGGPGSIVHWHLE 127

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLAVDEE 155
           YEK  +++    +LL  CV +S++I+ HLL+ +EE
Sbjct: 128 YEKISDEVAHPETLLQFCVEVSQEIDEHLLSEEEE 162

[Close]
BLAST of CmaCh06G016010 vs. TAIR10
Match: AT5G28010.1 (AT5G28010.1 Polyketide cyclase/dehydrase and lipid transport superfamily protein)

HSP 1 Score: 162.9 bits (411), Expect = 2.3e-40
Identity = 76/151 (50.33%), Postives = 104/151 (68.87%), Query Frame = 1 

Query: 2   SLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYC 61
           SL GKLE +V+IK PA+ F+ ++  +PHH++KA    +Q C L +GEWG VGSI+YWNY 
Sbjct: 14  SLLGKLEVEVEIKAPAAIFYHIYAGRPHHVAKATPRNVQSCDLHDGEWGTVGSIVYWNYV 73

Query: 62  HGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKD--KGSVVHWIFE 121
           H G+A VAKE +E V+ E   I F+V+EGD++ +YKSF ITIQ  PK+   GSVV W  E
Sbjct: 74  HEGQAKVAKERIELVEPEKKLIKFRVIEGDVMAEYKSFLITIQVTPKEGGTGSVVKWHIE 133

Query: 122 YEKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           YEK  E +P   +LL     ++K+I+ HLL+
Sbjct: 134 YEKIDENVPHPENLLPFFAEMTKEIDEHLLS 164

[Close]
BLAST of CmaCh06G016010 vs. NCBI nr
Match: gi|449445429|ref|XP_004140475.1| (PREDICTED: MLP-like protein 31 [Cucumis sativus])

HSP 1 Score: 231.1 bits (588), Expect = 2.0e-57
Identity = 101/148 (68.24%), Postives = 122/148 (82.43%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           MS+  KLE DV+I+ PASKFHE+ H++ HH+SKA GDK+Q C+L EG+WGKVGSIIYWNY
Sbjct: 1   MSVLRKLEADVEIEAPASKFHELLHKRLHHVSKASGDKVQSCELHEGDWGKVGSIIYWNY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
            H GKA V K+V+EAVDEENN I FKV++GDLLKDYK+F  TIQ +PK KGSV+HW  EY
Sbjct: 61  FHDGKAKVGKDVIEAVDEENNLISFKVIDGDLLKDYKTFNYTIQAVPKGKGSVIHWTMEY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHL 149
           EK HEK+ DSHS+L  C+AISKDI+ HL
Sbjct: 121 EKLHEKVADSHSMLQFCLAISKDIDVHL 148

[Close]
BLAST of CmaCh06G016010 vs. NCBI nr
Match: gi|5762258|dbj|BAA83470.1| (Csf-2 [Cucumis sativus])

HSP 1 Score: 226.1 bits (575), Expect = 6.3e-56
Identity = 102/150 (68.00%), Postives = 118/150 (78.67%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           MSLCGKLE DV I+  ASKFHEMFH+KPHHI     DKI G +LQEGEWG+VGSII W Y
Sbjct: 1   MSLCGKLEKDVPIRASASKFHEMFHKKPHHICNCSTDKIHGVELQEGEWGQVGSIICWKY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
            H GK  +AKE+VE VDEENN I FKV+EGDL + YK FR TI+CIPK+KGSV+HW+ EY
Sbjct: 61  FHEGKHKIAKEIVEHVDEENNSITFKVIEGDLTEHYKDFRFTIKCIPKEKGSVIHWVLEY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           EK H+KIPDSH+LL  CV +SKDI+  L A
Sbjct: 121 EKLHDKIPDSHTLLQFCVDVSKDIDKQLSA 150

[Close]
BLAST of CmaCh06G016010 vs. NCBI nr
Match: gi|778688513|ref|XP_011652767.1| (PREDICTED: MLP-like protein 28 [Cucumis sativus])

HSP 1 Score: 226.1 bits (575), Expect = 6.3e-56
Identity = 102/150 (68.00%), Postives = 118/150 (78.67%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           MSLCGKLE DV I+  ASKFHEMFH+KPHHI     DKI G +LQEGEWG+VGSII W Y
Sbjct: 1   MSLCGKLEKDVPIRASASKFHEMFHKKPHHICNCSTDKIHGVELQEGEWGQVGSIICWKY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
            H GK  +AKE+VE VDEENN I FKV+EGDL + YK FR TI+CIPK+KGSV+HW+ EY
Sbjct: 61  FHEGKHKIAKEIVEHVDEENNSITFKVIEGDLTEHYKDFRFTIKCIPKEKGSVIHWVLEY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           EK H+KIPDSH+LL  CV +SKDI+  L A
Sbjct: 121 EKLHDKIPDSHTLLQFCVDVSKDIDKQLSA 150

[Close]
BLAST of CmaCh06G016010 vs. NCBI nr
Match: gi|700202099|gb|KGN57232.1| (Csf-2 protein [Cucumis sativus])

HSP 1 Score: 223.8 bits (569), Expect = 3.1e-55
Identity = 101/151 (66.89%), Postives = 120/151 (79.47%), Query Frame = 1 

Query: 3   LCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNYCH 62
           L GKLETDV IK  AS FHE+FH+KPHHIS A  DKI G  L EGEWG+VGSII W Y H
Sbjct: 4   LYGKLETDVPIKASASMFHEIFHKKPHHISNASTDKIHGVDLHEGEWGQVGSIICWKYFH 63

Query: 63  GGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEYEK 122
            GKA +AKE++E VDEENN I FKV+EGDL + YK FR+TIQCIPK+KGSV+HWI EYEK
Sbjct: 64  DGKARIAKEIIEHVDEENNSITFKVIEGDLTEHYKDFRLTIQCIPKEKGSVIHWILEYEK 123

Query: 123 RHEKIPDSHSLLHLCVAISKDIEAHLLAVDE 154
            H++IPDSH+LL  CV +S+DI++ L  +DE
Sbjct: 124 LHDQIPDSHTLLQFCVEVSQDIDSMLSDIDE 154

[Close]
BLAST of CmaCh06G016010 vs. NCBI nr
Match: gi|659077118|ref|XP_008439042.1| (PREDICTED: MLP-like protein 43 isoform X2 [Cucumis melo])

HSP 1 Score: 223.0 bits (567), Expect = 5.3e-55
Identity = 102/150 (68.00%), Postives = 116/150 (77.33%), Query Frame = 1 

Query: 1   MSLCGKLETDVKIKVPASKFHEMFHRKPHHISKACGDKIQGCQLQEGEWGKVGSIIYWNY 60
           M   GKLE DV I+ PASKFHEMFH+KPHHIS A  DKI G  L EGEWG+VGSII W Y
Sbjct: 1   MGRYGKLEIDVPIRAPASKFHEMFHKKPHHISNASSDKIHGVDLHEGEWGQVGSIICWKY 60

Query: 61  CHGGKASVAKEVVEAVDEENNKICFKVVEGDLLKDYKSFRITIQCIPKDKGSVVHWIFEY 120
              GKA +AKE++E VDEENN I FKV+EGDL + YK F++TIQCIPK KGSVVHWI EY
Sbjct: 61  FSDGKARIAKEIIEHVDEENNSIAFKVIEGDLTEHYKDFKLTIQCIPKGKGSVVHWILEY 120

Query: 121 EKRHEKIPDSHSLLHLCVAISKDIEAHLLA 151
           EK H+KIPDSH+LL  CV +SKDI+  L A
Sbjct: 121 EKLHDKIPDSHTLLQFCVEVSKDIDKQLCA 150

The following BLAST results are available for this feature:
BLAST of CmaCh06G016010 vs. Swiss-Prot
Match NameE-valueIdentityDescription
MLP31_ARATH2.0e-4961.33MLP-like protein 31 OS=Arabidopsis thaliana GN=MLP31 PE=2 SV=2[more]
MLP28_ARATH1.3e-4858.71MLP-like protein 28 OS=Arabidopsis thaliana GN=MLP28 PE=1 SV=1[more]
MLP43_ARATH1.2e-4657.62MLP-like protein 43 OS=Arabidopsis thaliana GN=MLP43 PE=1 SV=1[more]
MLP34_ARATH2.0e-4656.13MLP-like protein 34 OS=Arabidopsis thaliana GN=MLP34 PE=2 SV=1[more]
KIRO_ACTDE1.9e-2841.61Kirola OS=Actinidia deliciosa PE=1 SV=1[more]
BLAST of CmaCh06G016010 vs. TrEMBL
Match NameE-valueIdentityDescription
M4CUQ4_BRARP2.6e-5648.05Uncharacterized protein OS=Brassica rapa subsp. pekinensis PE=4 SV=1[more]
Q9SXL8_CUCSA4.4e-5668.00Csf-2 protein OS=Cucumis sativus GN=Csf-2 PE=2 SV=1[more]
A0A0A0L9B6_CUCSA2.2e-5566.89Csf-2 protein OS=Cucumis sativus GN=Csa_3G172375 PE=4 SV=1[more]
A0A0A0L885_CUCSA7.0e-5468.75Uncharacterized protein OS=Cucumis sativus GN=Csa_3G171860 PE=4 SV=1[more]
Q9AXU0_PRUPE1.5e-4861.64Major latex-like protein OS=Prunus persica GN=PRUPE_ppa012742mg PE=4 SV=1[more]
BLAST of CmaCh06G016010 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70840.11.1e-5061.33 MLP-like protein 31[more]
AT1G70830.17.1e-5058.71 MLP-like protein 28[more]
AT1G70890.16.7e-4857.62 MLP-like protein 43[more]
AT1G70850.11.1e-4756.13 MLP-like protein 34[more]
AT5G28010.12.3e-4050.33 Polyketide cyclase/dehydrase and lipid transport superfamily protein[more]
BLAST of CmaCh06G016010 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449445429|ref|XP_004140475.1|2.0e-5768.24PREDICTED: MLP-like protein 31 [Cucumis sativus][more]
gi|5762258|dbj|BAA83470.1|6.3e-5668.00Csf-2 [Cucumis sativus][more]
gi|778688513|ref|XP_011652767.1|6.3e-5668.00PREDICTED: MLP-like protein 28 [Cucumis sativus][more]
gi|700202099|gb|KGN57232.1|3.1e-5566.89Csf-2 protein [Cucumis sativus][more]
gi|659077118|ref|XP_008439042.1|5.3e-5568.00PREDICTED: MLP-like protein 43 isoform X2 [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000916Bet_v_I/MLP
IPR023393START-like_dom_sf
IPR024949Bet_v_I_allergen
Vocabulary: Biological Process
TermDefinition
GO:0006952defense response
GO:0009607response to biotic stimulus
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0009607 response to biotic stimulus
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G016010.1CmaCh06G016010.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 3..150
score: 5.5
IPR000916Bet v I/Major latex proteinSMARTSM01037Bet_v_1_2coord: 150..226
score: 1.7E-8coord: 2..149
score: 3.9
IPR023393START-like domainGENE3DG3DSA:3.30.530.20coord: 1..150
score: 5.3E-58coord: 151..226
score: 7.6
IPR024949Bet v I type allergenPRINTSPR00634BETALLERGENcoord: 86..99
score: 6.8E-5coord: 140..150
score: 6.8E-5coord: 4..24
score: 6.8E-5coord: 109..125
score: 6.8E-5coord: 67..86
score: 6.
NoneNo IPR availablePANTHERPTHR31907FAMILY NOT NAMEDcoord: 2..152
score: 1.9
NoneNo IPR availablePANTHERPTHR31907:SF4MLP-LIKE PROTEIN 165-RELATEDcoord: 2..152
score: 1.9
NoneNo IPR availableunknownSSF55961Bet v1-likecoord: 69..150
score: 3.67E-38coord: 1..35
score: 3.67E-38coord: 150..225
score: 7.64

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh06G016010CmoCh14G015740Cucurbita moschata (Rifu)cmacmoB797
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh06G016010CmaCh14G015390Cucurbita maxima (Rimu)cmacmaB284
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh06G016010Silver-seed gourdcarcmaB0974
CmaCh06G016010Cucumber (Chinese Long) v3cmacucB0965
CmaCh06G016010Watermelon (97103) v2cmawmbB856
CmaCh06G016010Wax gourdcmawgoB1006
CmaCh06G016010Cucumber (Gy14) v1cgycmaB0727
CmaCh06G016010Cucurbita moschata (Rifu)cmacmoB829
CmaCh06G016010Wild cucumber (PI 183967)cmacpiB826
CmaCh06G016010Cucumber (Chinese Long) v2cmacuB814
CmaCh06G016010Melon (DHL92) v3.5.1cmameB763
CmaCh06G016010Watermelon (Charleston Gray)cmawcgB737
CmaCh06G016010Watermelon (97103) v1cmawmB798
CmaCh06G016010Cucurbita pepo (Zucchini)cmacpeB837
CmaCh06G016010Cucurbita pepo (Zucchini)cmacpeB853
CmaCh06G016010Bottle gourd (USVL1VR-Ls)cmalsiB765
CmaCh06G016010Cucumber (Gy14) v2cgybcmaB427
CmaCh06G016010Melon (DHL92) v3.6.1cmamedB863