CmaCh06G015330 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh06G015330 vs. Swiss-Prot
Match: PME21_ARATH (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana GN=PME21 PE=2 SV=2)

HSP 1 Score: 553.5 bits (1425), Expect = 2.9e-156
Identity = 273/573 (47.64%), Postives = 384/573 (67.02%), Query Frame = 1

Query: 69  YDGGGCKNNKVALVGLLSLFLVALV------------NGASGAGEDSGSSNVAIKTICEP 128
           YD    +  +  ++ + S+ L+++V            +G S    +  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 129 TTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAID 188
           T YR+TCE+ L+K+  ++ DP EL+K  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 189 TCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGD 248
            C EL+DYA+D+L  +F+++   F  + +D  L NLRIWLS A++++ETCL+ F+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 249 TGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEP 308
            GE M   LKT+ E+T NGL ++ E+++ V  + +  GL    R+L A        E  P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPGL--NSRRLLA--------EGFP 244

Query: 309 SWVNDR-RGLLQATGA--KIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGV 368
           SWV+ R R LLQA  A   ++ ++VVA+DGSG+YKTI +ALQ VP+K N TFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 369 YQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFEN 428
           Y+E V V+KTM+ ++ IGDGP KT I+  KN+KDG +T++TAT A VG  FI K++ FEN
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 429 SAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAV 488
           +AG  KHQAVA+RVQSD SIF+NCR DGYQDTLYTH+HRQFFRDCTI+GTIDF+FG+AA 
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 489 ILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGR 548
           + QNC + VRKP+ NQ C +TA GR + +E TG V Q C I+ +P +  V+   K YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 549 PWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK 608
           PWK+YSRTIIM   I D +QP+GW PW GDF LKTLFY+E  N GPG+A  NRV W GIK
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 609 QITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
            ++ +  + FTP  +I+G+ WI   GVPY +G+
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmaCh06G015330 vs. Swiss-Prot
Match: PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)

HSP 1 Score: 552.7 bits (1423), Expect = 5.0e-156
Identity = 273/566 (48.23%), Postives = 380/566 (67.14%), Query Frame = 1

Query: 72  GGCKNNKVALVGLLSLFLVALVN--GASGAGEDSGSSNV----------AIKTICEPTTY 131
           G  K  K  + G+++  LV +V   G + +   S S  +          A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 132 RETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAKEAIDT 191
           +ETC  +L+K++ DS  P +LIK GF+  I  ++ +I  A+     +AA D   K A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 192 CDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT 251
           C++L++ A DDL    D   D FS+ ++++++++LR+WLSG++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 252 GEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPS 311
            + M  + KTS+E+T+NGL M+  +++L+          + G+  R K L  ED    PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYAR-KLLSAEDGI--PS 244

Query: 312 WVNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 371
           WV      L AT   ++ANVVVA DGSG+YKTI +AL  VP+ + K FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 372 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDG-TSTFKTATFAAVGTNFIGKDLWFENSAG 431
           V V K MT V  IGDGPTKTKIT   N+  G   T+ TAT A  G NF  K++ FEN+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 432 PEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQ 491
           PE HQAVALRV +D+++FYNC++DGYQDTLY H+HRQFFRDCT++GT+DFIFG+  V+LQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 492 NCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWK 551
           NC I VRKPM++Q C++TAQGR +++E TG+VLQNC I+ +P + PV+ + K YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 552 QYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQIT 611
           ++SRTIIM   IDD+I P GWLPW GDFAL TL+Y E++N GPG+ +  RVKW GIK+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 612 PKHAIDFTPGLFIRGNPWIRRTGVPY 623
           PK A+ FTP  F+RGN WI    VPY
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmaCh06G015330 vs. Swiss-Prot
Match: PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 1.0e-153
Identity = 269/571 (47.11%), Postives = 383/571 (67.08%), Query Frame = 1

Query: 69  YDGGGCKNNK-VALVGLLSLFLVALV---------NGASGAGEDSGSSNV-AIKTICEPT 128
           YD    K  K   ++ + S+ L+++V         N +  AG++  +++V AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 129 TYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDT 188
            Y+ETCE+ L K   D+ DP EL+K  F+  ++Q+      S T+ +   DP AK A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 189 CDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT 248
           C EL+DYAI +L  +F+++   F  ++VD  L  LRIWLS  +++++TCLD F+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 249 GEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPE-TGRQLRAKELDEEDEEEEP 308
           GE +   LKT+ ++T NGL MV E+++ +  +     +PE   R+L ++E         P
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ----IPEMNSRRLLSQEF--------P 244

Query: 309 SWVNDR-RGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQ 368
           SW++ R R LL A  ++++ ++VVA+DGSG+YKTI +AL  VP+K N TFV+++KEG+Y+
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYK 304

Query: 369 EQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSA 428
           E V V+++MT ++ IGDGP KT I+  K++KDG +T+KTAT A VG +FI K++ FEN+A
Sbjct: 305 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 364

Query: 429 GPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVIL 488
           G  KHQAVA+RV +D SIFYNC+ DGYQDTLY H+HRQF+RDCTI+GTIDF+FG+AA + 
Sbjct: 365 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 424

Query: 489 QNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPW 548
           QNC + VRKP+ NQ C +TA GR + +E TG VLQ C I  +P +  V+   KTYLGRPW
Sbjct: 425 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 484

Query: 549 KQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQI 608
           K+YSRTIIM   I D + PEGW PW G+F L TLFY+E  N GPGAA   RV W GIK++
Sbjct: 485 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 544

Query: 609 TPKHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
           + +  + FTP  +I+G+ WI   GVPY  G+
Sbjct: 545 SDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmaCh06G015330 vs. Swiss-Prot
Match: PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)

HSP 1 Score: 494.6 bits (1272), Expect = 1.6e-138
Identity = 254/566 (44.88%), Postives = 369/566 (65.19%), Query Frame = 1

Query: 73  GCKNNKVALVGLLSLFLV------ALVNGASGAGEDSG---SSNVAIKTICEPTTYRETC 132
           G K  K  + G +S FLV      A+V       +D      +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 133 EEALVKSNVDSKDPRELIKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAKEAIDTCDEL 192
             +L+ ++ DS DP +LIK GF   I+ +  ++  A+     +A  +P AK A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 193 IDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEKM 252
           +  AIDDL    D     FS+++++ ++++LR+WLSG++ +Q+TC+D+F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 253 TNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSWVN- 312
             + KTS+E+++N L MV  +++L+ +     GL     +   K L  ED    P+WV  
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLTGALAKYARKLLSTEDSI--PTWVGP 244

Query: 313 DRRGLLQATGA---KIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 372
           + R L+ A G     ++AN VVA+DG+G++KTIT AL  VP+ +   F+I++KEG+Y+E+
Sbjct: 245 EARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEK 304

Query: 373 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDG-TSTFKTATFAAVGTNFIGKDLWFENSAG 432
           V V K M  V  IGDGP KT IT   NF  G   TF TAT    G +F  K++  EN+AG
Sbjct: 305 VTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAG 364

Query: 433 PEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQ 492
           PE  QAVALRV +D ++F++C++DG+QDTLY H+HRQF+RDCT++GT+DFIFG+A  ILQ
Sbjct: 365 PEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQ 424

Query: 493 NCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWK 552
           NCKI VRKP + Q C+VTAQGR   +E TG+VL  C I+ DP + P++ V K YLGRPWK
Sbjct: 425 NCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWK 484

Query: 553 QYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQIT 612
           ++SRTIIM+  IDD+I P GWLPW GDFALKTL+Y E  N GPG+ +  RVKW GIK++T
Sbjct: 485 EFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLT 544

Query: 613 PKHAIDFTPGLFIRGNPWIRRTGVPY 623
           P+ A+ +T   F+RG+ WI +T VPY
Sbjct: 545 PQDALLYTGDRFLRGDTWIPQTQVPY 564

BLAST of CmaCh06G015330 vs. Swiss-Prot
Match: PME46_ARATH (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana GN=PME46 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 8.1e-114
Identity = 236/567 (41.62%), Postives = 330/567 (58.20%), Query Frame = 1

Query: 78  KVALVGLLSLFLVALVNGA-----------SGAGEDSGSS-NVAIKTICEPTTYRETCEE 137
           ++A++ + S+ LV +V GA               E++G   +V++K +C+ T ++E C E
Sbjct: 24  RIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEPISVSVKALCDVTLHKEKCFE 83

Query: 138 ALVKS-NVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDELIDY 197
            L  + N     P EL K      I +L   +      +    D     A+  C ELI  
Sbjct: 84  TLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEHM--DNATSAAMGACVELIGL 143

Query: 198 AIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT----GEK 257
           A+D L  T      +F          +LR WLS   TYQETC+DA       +    GE 
Sbjct: 144 AVDQLNETMTSSLKNFD---------DLRTWLSSVGTYQETCMDALVEANKPSLTTFGE- 203

Query: 258 MTNLLKTSKEMTANGLVMVGEVTSLVSSL-WKALGLPETGR-QLRAKELDEEDEEEEPSW 317
             N LK S EMT+N L ++  +  +  ++ ++   L ETG  ++   +L           
Sbjct: 204 --NHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVADLP---------- 263

Query: 318 VNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQV 377
           + + R LL++   K +A +VVAKDGSGKY+TI +AL +V  K+ K  +IYVK+GVY E V
Sbjct: 264 MMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYLENV 323

Query: 378 MVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPE 437
            V+KT   V+M+GDG +KT ++AG NF DGT TF+TATFA  G  F+ +D+ F N+AGP 
Sbjct: 324 RVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTAGPA 383

Query: 438 KHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNC 497
           KHQAVAL V +D+S+FY C MD +QDT+Y HA RQF+RDC I GT+DFIFGNAAV+ Q C
Sbjct: 384 KHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVFQKC 443

Query: 498 KIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQY 557
           +I  R+PM+ Q+  +TAQGR +  + TGI + NC I        +    +T+LGRPWK +
Sbjct: 444 EILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI----QTFLGRPWKDF 503

Query: 558 SRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK-QITP 617
           S T+IM+  +D  I P+GWLPW GD A  T+FY E+ N GPGA+ +NRVKW+G+K  +T 
Sbjct: 504 STTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTK 562

Query: 618 KHAIDFTPGLFIRGNPWIRRTGVPYYS 625
           K A  FT   FI GN W+  T VP+ S
Sbjct: 564 KEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CmaCh06G015330 vs. TrEMBL
Match: A0A0A0L930_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_3G164510 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 9.3e-234
Identity = 392/571 (68.65%), Postives = 472/571 (82.66%), Query Frame = 1

Query: 75  KNNKVALVGLLSLFLVALV-----------NGASGAGEDSGSSNV-----AIKTICEPTT 134
           KN K AL+G+ SLFLVA+V             ASG G+ + ++ +     A+K +C+PT 
Sbjct: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65

Query: 135 YRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTC 194
           Y+ETCE+AL ++  ++ DPRELIKAGF+ A+ ++K AI NS TLK+AA+DPMAK+A+D C
Sbjct: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDAC 125

Query: 195 DELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTG 254
            EL+DYAIDDL+ +F +ITD+F + ++D+Y+++L++WLSGALTYQETC+D FENVTGDTG
Sbjct: 126 GELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTG 185

Query: 255 EKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSW 314
           EKMT LL+TSKE+T NGL MV EVTS+++S     GLP  GR+L  +E +E  + EEPSW
Sbjct: 186 EKMTKLLETSKELTINGLGMVSEVTSILTS----FGLPAIGRRLMTEESNE--QREEPSW 245

Query: 315 VNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQV 374
           V DRRGLLQATGA I+A+ VVAKDGSGKYKT+T AL DVP+KSNKTFVIYVK GVYQEQV
Sbjct: 246 VRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQV 305

Query: 375 MVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPE 434
           MV+K+MTWVMMIGDGPTKTKITAGKN+ DGT TFKTAT + +G+NFI KD+ FENSAG  
Sbjct: 306 MVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAA 365

Query: 435 KHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNC 494
           KHQAVALRVQSDMS+FYNC+MDGYQDTLYTHAHRQF+RDCTITGTIDFIFGN AV+ QNC
Sbjct: 366 KHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNC 425

Query: 495 KIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQY 554
           KI VRKPM+NQ+CIVTAQGR +RKEPT I+LQNCAISS P FFP+RH+ K +LGRPWKQY
Sbjct: 426 KILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQY 485

Query: 555 SRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITPK 614
           SRTIIMQ QIDDLIQPEGWLPW G+FAL TLFY E +NRGPGAA   RVKW+GIK+IT +
Sbjct: 486 SRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITME 545

Query: 615 HAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           HA+DFT   FIRG+PWI+ TGVPY SGM  +
Sbjct: 546 HALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570

BLAST of CmaCh06G015330 vs. TrEMBL
Match: W9S1C5_9ROSA (Pectinesterase OS=Morus notabilis GN=L484_007196 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 3.1e-189
Identity = 327/576 (56.77%), Postives = 427/576 (74.13%), Query Frame = 1

Query: 72  GGCKNNKVALVGLLSLFLVALVN----GASGAGEDSGSS------------NVAIKTICE 131
           GG +  K+A++G  +L LVA+V     G + +  DS S+              AI+ IC+
Sbjct: 10  GGQRKKKIAIIGTSALILVAMVVAVAVGFNRSESDSQSAAPTSPPPSVSTTTKAIQAICQ 69

Query: 132 PTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAI 191
           PT YRETCE +L  +  ++ DP+ELIKA F+ AI+ + AAI NS TLK+ A D    +A+
Sbjct: 70  PTDYRETCENSLTSAAGNTSDPKELIKAAFNVAIDHISAAIRNSSTLKELAKDSRTNQAL 129

Query: 192 DTCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTG 251
             CDEL+ YAIDDL  +FD+I   F   ++D Y+ +L+IWLSGA+TY++TCLD FEN TG
Sbjct: 130 SNCDELLKYAIDDLKESFDRI-GPFEYEKLDEYVDDLKIWLSGAITYEQTCLDGFENTTG 189

Query: 252 DTGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLP-ETGRQLRAKELDEEDEEE 311
           + G +M  +L++S+E+T+NGL MV E++S+++S      LP    R+L + E   + ++ 
Sbjct: 190 EAGLRMKAMLRSSQELTSNGLAMVTEISSILASY----NLPFNVSRRLLSSE---DGDDT 249

Query: 312 EPSWVND-RRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGV 371
           E SW++D +R LLQATG  I+A+VVVAKDGSGKY+T+ +AL+D+P+K NKTFVIY+K+GV
Sbjct: 250 ELSWLSDGKRRLLQATGKTIKADVVVAKDGSGKYRTVNEALKDIPKKGNKTFVIYIKQGV 309

Query: 372 YQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFEN 431
           Y+EQVM  K+MT VM+IGDGPTKT+IT   NF DG  TFKTAT + +G+NFI KD+ FEN
Sbjct: 310 YKEQVMFAKSMTHVMIIGDGPTKTRITGSLNFIDGVQTFKTATVSVIGSNFIAKDIGFEN 369

Query: 432 SAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAV 491
           SAGP+KHQAVALRVQSDMSIFYNC MDGYQDTLY H HRQF+RDC ITGTIDFIFG++A 
Sbjct: 370 SAGPQKHQAVALRVQSDMSIFYNCHMDGYQDTLYAHTHRQFYRDCVITGTIDFIFGDSAA 429

Query: 492 ILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGR 551
           + QNCK+ +RKP++NQ CIVTAQGR ER+E T +VLQNC I+ DP + PV+   K YLGR
Sbjct: 430 VFQNCKMVIRKPLDNQNCIVTAQGRTERREATALVLQNCTITGDPAYLPVKDQNKGYLGR 489

Query: 552 PWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK 611
           PWK++SRTIIM  +IDD+I PEGWLPW GDFAL T FYTE  NRGPG+    RVKWRGIK
Sbjct: 490 PWKEFSRTIIMNSRIDDVIAPEGWLPWAGDFALNTCFYTEILNRGPGSKLDGRVKWRGIK 549

Query: 612 QITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           ++  + A+++TPG FIRG+ WI+  GVPY SGM  +
Sbjct: 550 KVPAQRAVEYTPGRFIRGDWWIKAAGVPYVSGMLNV 577

BLAST of CmaCh06G015330 vs. TrEMBL
Match: A0A061EQE7_THECC (Pectinesterase OS=Theobroma cacao GN=TCM_021251 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 2.7e-185
Identity = 330/569 (58.00%), Postives = 425/569 (74.69%), Query Frame = 1

Query: 78  KVALVGLLSLFLVALV-----------------NGASGAGEDSGSSNVAIKTICEPTTYR 137
           KVA++ + S+FLVA+V                  GA+  GE S S+  AIK IC+PT YR
Sbjct: 125 KVAIISVCSVFLVAMVVAVAVGVSRSHGGRGNGGGATPTGEISTSTK-AIKAICQPTDYR 184

Query: 138 ETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDE 197
           ETCE++L  S+ ++ DP+ELIK GF  AI ++K  IANS T+K+ A DPMA++A++ C E
Sbjct: 185 ETCEKSL--SSANTTDPKELIKIGFQAAISEIKKVIANSSTVKEVAKDPMARQALENCHE 244

Query: 198 LIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEK 257
           L+DYAIDDL  +F+Q+ + F  +++D Y++NL+IWL GA+TYQ+TCLD F N TG+ G+K
Sbjct: 245 LMDYAIDDLKNSFNQLGE-FDFSKLDEYIENLKIWLGGAITYQQTCLDGFMNTTGEAGKK 304

Query: 258 MTNLLKTSKEMTANGLVMVGEVTSLVSSL-WKALGLPETG-RQLRAKELDEEDEEEEPSW 317
           M  LLKTS+E+T+NGL MV E++S++ +L  + +G   TG R+L A       E   P W
Sbjct: 305 MQALLKTSQELTSNGLAMVSEISSILGNLNIQNIGEISTGQRRLFA-------ENGFPMW 364

Query: 318 VN-DRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 377
           V+ ++R  ++ T A I+ NVVVAKDGSGKY +I +ALQ+VP+ +  TFVIY+K GVY+EQ
Sbjct: 365 VSSEQRSRIRQTPATIKPNVVVAKDGSGKYTSINEALQEVPKDNPTTFVIYIKAGVYKEQ 424

Query: 378 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGP 437
           V+V K+MT VM IGDG TKTKIT   N+ DGT TF+TAT   VG  FI KD+ FEN+AG 
Sbjct: 425 VIVTKSMTNVMFIGDGRTKTKITGDLNYADGTGTFRTATVGVVGDGFIAKDIGFENTAGA 484

Query: 438 EKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQN 497
            KHQAVALRVQSD SIFYNC+MDGYQDTLY H+HRQF+RDCTI+GTIDFIFG++A +LQN
Sbjct: 485 IKHQAVALRVQSDQSIFYNCQMDGYQDTLYAHSHRQFYRDCTISGTIDFIFGDSATVLQN 544

Query: 498 CKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQ 557
           CKI VRKP+ENQ+CIVTAQGR+ER+E + +VLQNC IS DP + PV+   K YLGRPWK+
Sbjct: 545 CKIIVRKPLENQQCIVTAQGRIERREVSALVLQNCTISGDPEYLPVKDKNKAYLGRPWKE 604

Query: 558 YSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITP 617
           +SRT+IMQ QIDD+I PEGWLPW GDFAL TL+Y EF NRGPGA + +RV WRGIK+I  
Sbjct: 605 FSRTLIMQSQIDDIIAPEGWLPWNGDFALNTLWYAEFGNRGPGAVQTHRVTWRGIKKINA 664

Query: 618 KHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
             A  FTPG+F+RG+ WI + GVPY  G+
Sbjct: 665 AIARRFTPGVFLRGDDWIPKAGVPYTPGL 682

BLAST of CmaCh06G015330 vs. TrEMBL
Match: F6H6V9_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_05s0077g01950 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 1.2e-183
Identity = 325/587 (55.37%), Postives = 419/587 (71.38%), Query Frame = 1

Query: 66  NMAYDGGGCKNNKVALVGLLSLFLVALV----------NGASGAGEDSG-------SSNV 125
           N A D       KVAL+G  S+ LVA+V          NG+S +  D         +S  
Sbjct: 4   NNAADADSRGKKKVALMGFSSILLVAMVVGTVVTVNHRNGSSSSATDDAQAGGGISTSVK 63

Query: 126 AIKTICEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATD 185
           AI+ IC+PT Y+E C  +L  +  ++ DP+EL++  F  AI Q+ +A+ NS TL+    D
Sbjct: 64  AIQAICQPTDYKEACVNSLTSAKANTSDPKELVRTAFQVAINQISSALQNSTTLRDLEKD 123

Query: 186 PMAKEAIDTCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLD 245
           P  K A++ C EL+DYAIDDL  +F+++   F ++++D+Y+++L+IWLSGALTYQETCLD
Sbjct: 124 PRTKGALENCHELMDYAIDDLRNSFNKL-GVFDISKIDDYVEDLKIWLSGALTYQETCLD 183

Query: 246 AFENVTGDTGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELD 305
            FEN TGD GEKM  LLK++ E+T+NGL M+ E++S++++L     +P   R+L + E  
Sbjct: 184 GFENTTGDAGEKMKALLKSAGELTSNGLAMIDEISSVLTNLQ----IPGISRRLLSDESG 243

Query: 306 EEDEEEE-----PSWVN-DRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSN 365
           + +   +     PSW +  +R L QAT   I+ NV+VA+DGSGKYKTI +AL ++P+  N
Sbjct: 244 KGEYRSDEGGLYPSWASVGQRKLFQATPDTIKPNVIVAQDGSGKYKTINEALVEIPKNGN 303

Query: 366 KTFVIYVKEGVYQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGT 425
            TFV+YVKEGVY+EQV   K+MT VM+IGDGPTKT I+   NF DG  TF+TAT AAVG+
Sbjct: 304 TTFVLYVKEGVYKEQVNFTKSMTNVMLIGDGPTKTTISGSLNFIDGIGTFRTATVAAVGS 363

Query: 426 NFIGKDLWFENSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITG 485
           NF+ KD+ FEN+AG  KHQAVALRV SDM+IFYNCRMDGYQDTLY HAHRQF+RDCTITG
Sbjct: 364 NFMAKDIGFENNAGASKHQAVALRVGSDMAIFYNCRMDGYQDTLYVHAHRQFYRDCTITG 423

Query: 486 TIDFIFGNAAVILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFP 545
           TIDFIFG++AV+ QNC I VRKP++NQ+CIVTAQGR ER+EPTGIVLQNC IS+   + P
Sbjct: 424 TIDFIFGDSAVVFQNCLILVRKPLDNQQCIVTAQGRNERREPTGIVLQNCTISAADDYIP 483

Query: 546 VRHVFKTYLGRPWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAA 605
            +  FK+YLGRPWK +SRTIIMQ QIDDLI PEGWLPW GDF L T FY E+ NRGP +A
Sbjct: 484 FKTKFKSYLGRPWKAFSRTIIMQSQIDDLISPEGWLPWMGDFGLNTCFYAEYGNRGPASA 543

Query: 606 KQNRVKWRGIKQITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
             +RV WRGIKQIT +H  DFT G FI G+ W+  +GVPY S M  +
Sbjct: 544 TTSRVTWRGIKQITGQHVNDFTVGRFISGHLWLGASGVPYTSDMMAV 585

BLAST of CmaCh06G015330 vs. TrEMBL
Match: D7U9X3_VITVI (Pectinesterase OS=Vitis vinifera GN=VIT_14s0060g01950 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 2.8e-182
Identity = 314/577 (54.42%), Postives = 420/577 (72.79%), Query Frame = 1

Query: 69  YDGGGCKNNKVALVGLLSLFLVALV----NGASGAGEDSG--------SSNVAIKTICEP 128
           Y+    K  K+A++G  S+FLVA+V       S +  DSG        SS  AI+ IC+P
Sbjct: 6   YNNDRGKKKKLAIIGFSSIFLVAMVIAVIASVSSSSSDSGNAQKQEISSSMKAIQAICQP 65

Query: 129 TTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAID 188
           T Y++ C  +L     ++ DP++L++A F  A+E L AA  NS  L++   DP A +A+ 
Sbjct: 66  TDYKDACVNSLTSKAGNTTDPKDLVQAAFASAMEHLSAAAKNSTLLQELNKDPRASQALQ 125

Query: 189 TCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGD 248
            C++L++YAIDDL  +F+Q+ D F  +++DN + +++IWLS  +TYQETCLD FEN TGD
Sbjct: 126 NCEDLVNYAIDDLKKSFNQVGD-FDYSKMDNIIADIKIWLSAVITYQETCLDGFENTTGD 185

Query: 249 TGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEED---EE 308
            GEKM  +LKTS E+++NGL +VGEV+S++S+L     L    R+L + +  + D   ++
Sbjct: 186 AGEKMRQILKTSMELSSNGLAIVGEVSSILSNLQ----LANLNRRLLSDDPADPDNHIDD 245

Query: 309 EEPSWVNDR-RGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEG 368
           E P W +   R LLQA  ++++ N+ VAKDGSG +KTI +A++ +P+ SN+TF++Y+K+G
Sbjct: 246 EFPYWSHSEGRKLLQANVSELKPNLTVAKDGSGDFKTINEAIRQLPKFSNQTFILYIKKG 305

Query: 369 VYQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFE 428
           +Y+EQV ++KT T +MM+GDGPTKTKIT   NF DGT TFKTAT A +G  FI K + FE
Sbjct: 306 IYEEQVQINKTFTNLMMVGDGPTKTKITGSLNFVDGTPTFKTATVAVLGDGFIAKGIGFE 365

Query: 429 NSAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAA 488
           NSAG  KHQAVALRVQSD SIFYNC+MDGYQDTLYTH  RQF+RDCTI+GTIDFIFG+AA
Sbjct: 366 NSAGAAKHQAVALRVQSDRSIFYNCQMDGYQDTLYTHTKRQFYRDCTISGTIDFIFGDAA 425

Query: 489 VILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLG 548
           VI QNC   VRKP++NQ+CIVTAQGR ER++P+ I++QN   ++DP ++P R+  K+YLG
Sbjct: 426 VIFQNCTFVVRKPLDNQQCIVTAQGRKERRQPSAIIIQNSTFTADPEYYPYRNELKSYLG 485

Query: 549 RPWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGI 608
           RPWK++SRTIIM+  I+DLIQP GWLPW GDFAL+T FYTEF NRGPGA   +RVKWRGI
Sbjct: 486 RPWKEFSRTIIMESYIEDLIQPSGWLPWAGDFALRTCFYTEFRNRGPGAKTHDRVKWRGI 545

Query: 609 KQITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           K I P HAIDF PG F+ G+ WI  TGVPY SG+ T+
Sbjct: 546 KTIKPSHAIDFAPGRFLSGDRWIPSTGVPYNSGLFTL 577

BLAST of CmaCh06G015330 vs. TAIR10
Match: AT3G05610.1 (AT3G05610.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 553.5 bits (1425), Expect = 1.7e-157
Identity = 273/573 (47.64%), Postives = 384/573 (67.02%), Query Frame = 1

Query: 69  YDGGGCKNNKVALVGLLSLFLVALV------------NGASGAGEDSGSSNVAIKTICEP 128
           YD    +  +  ++ + S+ L+++V            +G S    +  +S  A+K +C P
Sbjct: 5   YDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAP 64

Query: 129 TTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAID 188
           T YR+TCE+ L+K+  ++ DP EL+K  F+  ++Q+  A   S T+ +   D   + A+D
Sbjct: 65  TDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD 124

Query: 189 TCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGD 248
            C EL+DYA+D+L  +F+++   F  + +D  L NLRIWLS A++++ETCL+ F+   G+
Sbjct: 125 QCKELMDYALDELSNSFEEL-GKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGN 184

Query: 249 TGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEP 308
            GE M   LKT+ E+T NGL ++ E+++ V  + +  GL    R+L A        E  P
Sbjct: 185 AGETMKKALKTAIELTHNGLAIISEMSNFVGQM-QIPGL--NSRRLLA--------EGFP 244

Query: 309 SWVNDR-RGLLQATGA--KIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGV 368
           SWV+ R R LLQA  A   ++ ++VVA+DGSG+YKTI +ALQ VP+K N TFV+++K G+
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGL 304

Query: 369 YQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFEN 428
           Y+E V V+KTM+ ++ IGDGP KT I+  KN+KDG +T++TAT A VG  FI K++ FEN
Sbjct: 305 YKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFEN 364

Query: 429 SAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAV 488
           +AG  KHQAVA+RVQSD SIF+NCR DGYQDTLYTH+HRQFFRDCTI+GTIDF+FG+AA 
Sbjct: 365 TAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAA 424

Query: 489 ILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGR 548
           + QNC + VRKP+ NQ C +TA GR + +E TG V Q C I+ +P +  V+   K YLGR
Sbjct: 425 VFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGR 484

Query: 549 PWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK 608
           PWK+YSRTIIM   I D +QP+GW PW GDF LKTLFY+E  N GPG+A  NRV W GIK
Sbjct: 485 PWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIK 544

Query: 609 QITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
            ++ +  + FTP  +I+G+ WI   GVPY +G+
Sbjct: 545 TLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

BLAST of CmaCh06G015330 vs. TAIR10
Match: AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 552.7 bits (1423), Expect = 2.8e-157
Identity = 273/566 (48.23%), Postives = 380/566 (67.14%), Query Frame = 1

Query: 72  GGCKNNKVALVGLLSLFLVALVN--GASGAGEDSGSSNV----------AIKTICEPTTY 131
           G  K  K  + G+++  LV +V   G + +   S S  +          A++ +C PT Y
Sbjct: 5   GELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDY 64

Query: 132 RETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAKEAIDT 191
           +ETC  +L+K++ DS  P +LIK GF+  I  ++ +I  A+     +AA D   K A++ 
Sbjct: 65  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 124

Query: 192 CDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT 251
           C++L++ A DDL    D   D FS+ ++++++++LR+WLSG++ YQ+TC+D FE      
Sbjct: 125 CEKLMNDATDDLKKCLDNF-DGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKL 184

Query: 252 GEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPS 311
            + M  + KTS+E+T+NGL M+  +++L+          + G+  R K L  ED    PS
Sbjct: 185 SQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYAR-KLLSAEDGI--PS 244

Query: 312 WVNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 371
           WV      L AT   ++ANVVVA DGSG+YKTI +AL  VP+ + K FVIY+K+GVY E+
Sbjct: 245 WVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEK 304

Query: 372 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDG-TSTFKTATFAAVGTNFIGKDLWFENSAG 431
           V V K MT V  IGDGPTKTKIT   N+  G   T+ TAT A  G NF  K++ FEN+AG
Sbjct: 305 VDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAG 364

Query: 432 PEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQ 491
           PE HQAVALRV +D+++FYNC++DGYQDTLY H+HRQFFRDCT++GT+DFIFG+  V+LQ
Sbjct: 365 PEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQ 424

Query: 492 NCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWK 551
           NC I VRKPM++Q C++TAQGR +++E TG+VLQNC I+ +P + PV+ + K YLGRPWK
Sbjct: 425 NCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWK 484

Query: 552 QYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQIT 611
           ++SRTIIM   IDD+I P GWLPW GDFAL TL+Y E++N GPG+ +  RVKW GIK+++
Sbjct: 485 EFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLS 544

Query: 612 PKHAIDFTPGLFIRGNPWIRRTGVPY 623
           PK A+ FTP  F+RGN WI    VPY
Sbjct: 545 PKQALRFTPARFLRGNLWIPPNRVPY 566

BLAST of CmaCh06G015330 vs. TAIR10
Match: AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 545.0 bits (1403), Expect = 5.9e-155
Identity = 269/571 (47.11%), Postives = 383/571 (67.08%), Query Frame = 1

Query: 69  YDGGGCKNNK-VALVGLLSLFLVALV---------NGASGAGEDSGSSNV-AIKTICEPT 128
           YD    K  K   ++ + S+ L+++V         N +  AG++  +++V AIK +C PT
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPT 64

Query: 129 TYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDT 188
            Y+ETCE+ L K   D+ DP EL+K  F+  ++Q+      S T+ +   DP AK A+D 
Sbjct: 65  DYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALDQ 124

Query: 189 CDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT 248
           C EL+DYAI +L  +F+++   F  ++VD  L  LRIWLS  +++++TCLD F+   G+ 
Sbjct: 125 CKELMDYAIGELSKSFEEL-GKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNA 184

Query: 249 GEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPE-TGRQLRAKELDEEDEEEEP 308
           GE +   LKT+ ++T NGL MV E+++ +  +     +PE   R+L ++E         P
Sbjct: 185 GETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQ----IPEMNSRRLLSQEF--------P 244

Query: 309 SWVNDR-RGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQ 368
           SW++ R R LL A  ++++ ++VVA+DGSG+YKTI +AL  VP+K N TFV+++KEG+Y+
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYK 304

Query: 369 EQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSA 428
           E V V+++MT ++ IGDGP KT I+  K++KDG +T+KTAT A VG +FI K++ FEN+A
Sbjct: 305 EYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTA 364

Query: 429 GPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVIL 488
           G  KHQAVA+RV +D SIFYNC+ DGYQDTLY H+HRQF+RDCTI+GTIDF+FG+AA + 
Sbjct: 365 GAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVF 424

Query: 489 QNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPW 548
           QNC + VRKP+ NQ C +TA GR + +E TG VLQ C I  +P +  V+   KTYLGRPW
Sbjct: 425 QNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPW 484

Query: 549 KQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQI 608
           K+YSRTIIM   I D + PEGW PW G+F L TLFY+E  N GPGAA   RV W GIK++
Sbjct: 485 KEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKL 544

Query: 609 TPKHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
           + +  + FTP  +I+G+ WI   GVPY  G+
Sbjct: 545 SDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

BLAST of CmaCh06G015330 vs. TAIR10
Match: AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 494.6 bits (1272), Expect = 9.1e-140
Identity = 254/566 (44.88%), Postives = 369/566 (65.19%), Query Frame = 1

Query: 73  GCKNNKVALVGLLSLFLV------ALVNGASGAGEDSG---SSNVAIKTICEPTTYRETC 132
           G K  K  + G +S FLV      A+V       +D      +  A++ +C PT +++TC
Sbjct: 5   GDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPKDDENHIRKTTKAVQAVCAPTDFKDTC 64

Query: 133 EEALVKSNVDSKDPRELIKAGFHFAIEQLKAAI--ANSPTLKQAATDPMAKEAIDTCDEL 192
             +L+ ++ DS DP +LIK GF   I+ +  ++  A+     +A  +P AK A + C++L
Sbjct: 65  VNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAFELCEKL 124

Query: 193 IDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEKM 252
           +  AIDDL    D     FS+++++ ++++LR+WLSG++ +Q+TC+D+F  +  +  + M
Sbjct: 125 MIDAIDDLKKCMDH---GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSNLMQDM 184

Query: 253 TNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSWVN- 312
             + KTS+E+++N L MV  +++L+ +     GL     +   K L  ED    P+WV  
Sbjct: 185 LKIFKTSRELSSNSLAMVTRISTLIPNS-NLTGLTGALAKYARKLLSTEDSI--PTWVGP 244

Query: 313 DRRGLLQATGA---KIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 372
           + R L+ A G     ++AN VVA+DG+G++KTIT AL  VP+ +   F+I++KEG+Y+E+
Sbjct: 245 EARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGNKVPFIIHIKEGIYKEK 304

Query: 373 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDG-TSTFKTATFAAVGTNFIGKDLWFENSAG 432
           V V K M  V  IGDGP KT IT   NF  G   TF TAT    G +F  K++  EN+AG
Sbjct: 305 VTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGIENTAG 364

Query: 433 PEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQ 492
           PE  QAVALRV +D ++F++C++DG+QDTLY H+HRQF+RDCT++GT+DFIFG+A  ILQ
Sbjct: 365 PEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDAKCILQ 424

Query: 493 NCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWK 552
           NCKI VRKP + Q C+VTAQGR   +E TG+VL  C I+ DP + P++ V K YLGRPWK
Sbjct: 425 NCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYLGRPWK 484

Query: 553 QYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQIT 612
           ++SRTIIM+  IDD+I P GWLPW GDFALKTL+Y E  N GPG+ +  RVKW GIK++T
Sbjct: 485 EFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPGIKKLT 544

Query: 613 PKHAIDFTPGLFIRGNPWIRRTGVPY 623
           P+ A+ +T   F+RG+ WI +T VPY
Sbjct: 545 PQDALLYTGDRFLRGDTWIPQTQVPY 564

BLAST of CmaCh06G015330 vs. TAIR10
Match: AT5G04960.1 (AT5G04960.1 Plant invertase/pectin methylesterase inhibitor superfamily)

HSP 1 Score: 412.5 bits (1059), Expect = 4.6e-115
Identity = 236/567 (41.62%), Postives = 330/567 (58.20%), Query Frame = 1

Query: 78  KVALVGLLSLFLVALVNGA-----------SGAGEDSGSS-NVAIKTICEPTTYRETCEE 137
           ++A++ + S+ LV +V GA               E++G   +V++K +C+ T ++E C E
Sbjct: 24  RIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEPISVSVKALCDVTLHKEKCFE 83

Query: 138 ALVKS-NVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDELIDY 197
            L  + N     P EL K      I +L   +      +    D     A+  C ELI  
Sbjct: 84  TLGSAPNASRSSPEELFKYAVKVTITELSKVLDGFSNGEHM--DNATSAAMGACVELIGL 143

Query: 198 AIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDT----GEK 257
           A+D L  T      +F          +LR WLS   TYQETC+DA       +    GE 
Sbjct: 144 AVDQLNETMTSSLKNFD---------DLRTWLSSVGTYQETCMDALVEANKPSLTTFGE- 203

Query: 258 MTNLLKTSKEMTANGLVMVGEVTSLVSSL-WKALGLPETGR-QLRAKELDEEDEEEEPSW 317
             N LK S EMT+N L ++  +  +  ++ ++   L ETG  ++   +L           
Sbjct: 204 --NHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVADLP---------- 263

Query: 318 VNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQV 377
           + + R LL++   K +A +VVAKDGSGKY+TI +AL +V  K+ K  +IYVK+GVY E V
Sbjct: 264 MMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYLENV 323

Query: 378 MVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPE 437
            V+KT   V+M+GDG +KT ++AG NF DGT TF+TATFA  G  F+ +D+ F N+AGP 
Sbjct: 324 RVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTAGPA 383

Query: 438 KHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNC 497
           KHQAVAL V +D+S+FY C MD +QDT+Y HA RQF+RDC I GT+DFIFGNAAV+ Q C
Sbjct: 384 KHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVFQKC 443

Query: 498 KIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQY 557
           +I  R+PM+ Q+  +TAQGR +  + TGI + NC I        +    +T+LGRPWK +
Sbjct: 444 EILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI----QTFLGRPWKDF 503

Query: 558 SRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK-QITP 617
           S T+IM+  +D  I P+GWLPW GD A  T+FY E+ N GPGA+ +NRVKW+G+K  +T 
Sbjct: 504 STTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTK 562

Query: 618 KHAIDFTPGLFIRGNPWIRRTGVPYYS 625
           K A  FT   FI GN W+  T VP+ S
Sbjct: 564 KEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CmaCh06G015330 vs. NCBI nr
Match: gi|659076869|ref|XP_008438906.1| (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 835.1 bits (2156), Expect = 8.1e-239
Identity = 401/576 (69.62%), Postives = 479/576 (83.16%), Query Frame = 1

Query: 70  DGGGCKNNKVALVGLLSLFLVALV----------------NGASGAGEDSGSSNVAIKTI 129
           DGG  KN K AL+G+ SLFLVA+V                +G  GA  +  +S  A++ +
Sbjct: 3   DGG--KNKKAALIGVSSLFLVAMVVAVAVGVNRKNPTTDSSGGDGATAEISTSTKAVQAL 62

Query: 130 CEPTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKE 189
           C+PT Y+ETCE+AL ++  ++ DPRELIK+GFH A+ ++K AI NS  LK+AA+DPMAK+
Sbjct: 63  CQPTDYKETCEKALSEAGTNTSDPRELIKSGFHVAVNEIKWAIGNSTQLKEAASDPMAKQ 122

Query: 190 AIDTCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENV 249
           A+D C EL+DYAIDDL+ +FD+ITD+F + ++++Y+++L+IWLSGALTYQETC+D FENV
Sbjct: 123 ALDACGELMDYAIDDLVISFDRITDNFDVQKLNDYVEDLKIWLSGALTYQETCIDGFENV 182

Query: 250 TGDTGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEE 309
           TGDTGEKM  LLKTSKE+T+NGL MV EV S+++S     GLP  GR+L  +E +++  E
Sbjct: 183 TGDTGEKMAKLLKTSKELTSNGLDMVSEVNSIMTSF----GLPAVGRRLLTEESNQQ--E 242

Query: 310 EEPSWVNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGV 369
           EEPSWV DRRGLLQATGA ++A+VVVAKDGSGKYKT+T+AL DVP+KSNKTFVI+VK GV
Sbjct: 243 EEPSWVGDRRGLLQATGATVKADVVVAKDGSGKYKTVTEALNDVPKKSNKTFVIHVKAGV 302

Query: 370 YQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFEN 429
           YQEQVMVDK+MTWVMMIGDGPTKTKITAGKN+ DGT TFKTAT + +G+NFI KD+ FEN
Sbjct: 303 YQEQVMVDKSMTWVMMIGDGPTKTKITAGKNYVDGTPTFKTATVSVIGSNFIAKDIGFEN 362

Query: 430 SAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAV 489
           SAG  KHQAVALRVQSDMSIFYNC+MDGYQDTLYTHAHRQF+RDCTITGTIDFIFGNAA 
Sbjct: 363 SAGAAKHQAVALRVQSDMSIFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNAAA 422

Query: 490 ILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGR 549
           + QNCKI VRKPM+NQ+CIVTAQGR +RKEPT I+LQNCAISSDP FFPVRH+ K YLGR
Sbjct: 423 VFQNCKILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSDPTFFPVRHINKAYLGR 482

Query: 550 PWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK 609
           PWKQYSRTIIMQCQIDDLIQPEGWLPW GDFAL TLFY E +NRGPGAA   RVKW+GIK
Sbjct: 483 PWKQYSRTIIMQCQIDDLIQPEGWLPWMGDFALHTLFYAEINNRGPGAATDKRVKWKGIK 542

Query: 610 QITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           +ITP+HA+DFT G FIRG+PWI+ TGVPY SGM  +
Sbjct: 543 KITPQHALDFTVGRFIRGDPWIKPTGVPYSSGMMAV 570

BLAST of CmaCh06G015330 vs. NCBI nr
Match: gi|778678954|ref|XP_004134493.2| (PREDICTED: pectinesterase-like [Cucumis sativus])

HSP 1 Score: 817.8 bits (2111), Expect = 1.3e-233
Identity = 392/571 (68.65%), Postives = 472/571 (82.66%), Query Frame = 1

Query: 75  KNNKVALVGLLSLFLVALV-----------NGASGAGEDSGSSNV-----AIKTICEPTT 134
           KN K AL+G+ SLFLVA+V             ASG G+ + ++ +     A+K +C+PT 
Sbjct: 6   KNKKAALIGVSSLFLVAMVVAVAVGVNRNSPNASGGGDSAATAEISTSTKAVKALCQPTD 65

Query: 135 YRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTC 194
           Y+ETCE+AL ++  ++ DPRELIKAGF+ A+ ++K AI NS TLK+AA+DPMAK+A+D C
Sbjct: 66  YQETCEKALSEAGTNTSDPRELIKAGFNVAVNEIKWAIGNSTTLKEAASDPMAKQALDAC 125

Query: 195 DELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTG 254
            EL+DYAIDDL+ +F +ITD+F + ++D+Y+++L++WLSGALTYQETC+D FENVTGDTG
Sbjct: 126 GELMDYAIDDLVISFQRITDNFDMQKLDDYIEDLKVWLSGALTYQETCIDGFENVTGDTG 185

Query: 255 EKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEPSW 314
           EKMT LL+TSKE+T NGL MV EVTS+++S     GLP  GR+L  +E +E  + EEPSW
Sbjct: 186 EKMTKLLETSKELTINGLGMVSEVTSILTS----FGLPAIGRRLMTEESNE--QREEPSW 245

Query: 315 VNDRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQV 374
           V DRRGLLQATGA I+A+ VVAKDGSGKYKT+T AL DVP+KSNKTFVIYVK GVYQEQV
Sbjct: 246 VRDRRGLLQATGANIKADAVVAKDGSGKYKTVTAALNDVPKKSNKTFVIYVKAGVYQEQV 305

Query: 375 MVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGPE 434
           MV+K+MTWVMMIGDGPTKTKITAGKN+ DGT TFKTAT + +G+NFI KD+ FENSAG  
Sbjct: 306 MVEKSMTWVMMIGDGPTKTKITAGKNYIDGTPTFKTATVSVIGSNFIAKDIGFENSAGAA 365

Query: 435 KHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQNC 494
           KHQAVALRVQSDMS+FYNC+MDGYQDTLYTHAHRQF+RDCTITGTIDFIFGN AV+ QNC
Sbjct: 366 KHQAVALRVQSDMSVFYNCQMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGNGAVVFQNC 425

Query: 495 KIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQY 554
           KI VRKPM+NQ+CIVTAQGR +RKEPT I+LQNCAISS P FFP+RH+ K +LGRPWKQY
Sbjct: 426 KILVRKPMDNQQCIVTAQGRTQRKEPTAIILQNCAISSAPDFFPIRHINKAFLGRPWKQY 485

Query: 555 SRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITPK 614
           SRTIIMQ QIDDLIQPEGWLPW G+FAL TLFY E +NRGPGAA   RVKW+GIK+IT +
Sbjct: 486 SRTIIMQSQIDDLIQPEGWLPWTGNFALNTLFYAEINNRGPGAATDKRVKWKGIKKITME 545

Query: 615 HAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           HA+DFT   FIRG+PWI+ TGVPY SGM  +
Sbjct: 546 HALDFTAARFIRGDPWIKPTGVPYTSGMMAV 570

BLAST of CmaCh06G015330 vs. NCBI nr
Match: gi|1009163283|ref|XP_015899883.1| (PREDICTED: probable pectinesterase/pectinesterase inhibitor 21 isoform X1 [Ziziphus jujuba])

HSP 1 Score: 698.4 bits (1801), Expect = 1.2e-197
Identity = 334/574 (58.19%), Postives = 435/574 (75.78%), Query Frame = 1

Query: 70  DGGGCKNNKVALVGLLSLFLVALV--------NGASGAGEDSGSSNV-----AIKTICEP 129
           DGG  K  KVA++G  +L LVA+V         G S   + S S ++     A+K IC P
Sbjct: 8   DGGNGKKKKVAIMGASALILVAMVVAVAVGVHKGGSDDDDHSSSGSISTSSKAVKQICAP 67

Query: 130 TTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAID 189
           T YRETCE +L  S+V+S DP+ELIKAGF  A++ L+ AI NS TLK+ A DP A +A+ 
Sbjct: 68  TDYRETCETSL--SSVNSTDPKELIKAGFQVAVQHLRVAIENSTTLKELAKDPRANQALS 127

Query: 190 TCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGD 249
            C+EL+DYAIDD+  +F+++  +F +++++ Y+++L++WL GA+TY++TCLD FEN TGD
Sbjct: 128 NCEELMDYAIDDIQDSFNRL-GAFDISKLEEYVEDLKVWLGGAVTYEQTCLDGFENTTGD 187

Query: 250 TGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLPETGRQLRAKELDEEDEEEEP 309
            G++M  +LKT++E+T+NGL MV E+ ++++S       P T      + L+EE   E P
Sbjct: 188 AGDRMKAILKTAQELTSNGLAMVTEIYNVLNSNLNLPNAPSTPNPPHRRLLNEE---ETP 247

Query: 310 SWVND-RRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQ 369
           SWVND +R LL A+ A ++ +VVVAKDGSGKYKT+ +AL+D+P+  N TFVIY+K GVY+
Sbjct: 248 SWVNDGKRKLLAASPADLKPDVVVAKDGSGKYKTVNEALKDIPKYGNNTFVIYIKAGVYE 307

Query: 370 EQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSA 429
           E V++DKTMT VMMIGDGPTK+ IT  KN+ DGT TFKTAT   +G NF  KD+ FENSA
Sbjct: 308 ETVIIDKTMTHVMMIGDGPTKSVITGSKNYVDGTLTFKTATVTIIGGNFTAKDMGFENSA 367

Query: 430 GPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVIL 489
           GPEKHQAVALRVQSD+S+FYNCRMDGYQDTLYTHAHRQF+RDCTITGTIDFIFG+A  + 
Sbjct: 368 GPEKHQAVALRVQSDLSLFYNCRMDGYQDTLYTHAHRQFYRDCTITGTIDFIFGDAGAVF 427

Query: 490 QNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPW 549
           QNC++ VRKP++NQ CIVTAQGR +R+ P+ I+LQNC IS DP + PV+ V K YLGRPW
Sbjct: 428 QNCQMIVRKPLDNQNCIVTAQGRSDRRTPSAIILQNCTISGDPLYMPVKDVNKAYLGRPW 487

Query: 550 KQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQI 609
           K++SRTIIMQ QIDD+IQP+GWLPW G FA+ + FYTEF+NRG GA    RVKW+GIK I
Sbjct: 488 KEFSRTIIMQSQIDDIIQPDGWLPWAGTFAINSCFYTEFNNRGAGADMSKRVKWKGIKTI 547

Query: 610 TPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           TP+HA+DFTPG F+ G+ WI+ TG+PY SGM  +
Sbjct: 548 TPQHAVDFTPGRFLGGDRWIKATGIPYVSGMINV 575

BLAST of CmaCh06G015330 vs. NCBI nr
Match: gi|703132462|ref|XP_010105131.1| (Putative pectinesterase/pectinesterase inhibitor 28 [Morus notabilis])

HSP 1 Score: 669.8 bits (1727), Expect = 4.5e-189
Identity = 327/576 (56.77%), Postives = 427/576 (74.13%), Query Frame = 1

Query: 72  GGCKNNKVALVGLLSLFLVALVN----GASGAGEDSGSS------------NVAIKTICE 131
           GG +  K+A++G  +L LVA+V     G + +  DS S+              AI+ IC+
Sbjct: 10  GGQRKKKIAIIGTSALILVAMVVAVAVGFNRSESDSQSAAPTSPPPSVSTTTKAIQAICQ 69

Query: 132 PTTYRETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAI 191
           PT YRETCE +L  +  ++ DP+ELIKA F+ AI+ + AAI NS TLK+ A D    +A+
Sbjct: 70  PTDYRETCENSLTSAAGNTSDPKELIKAAFNVAIDHISAAIRNSSTLKELAKDSRTNQAL 129

Query: 192 DTCDELIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTG 251
             CDEL+ YAIDDL  +FD+I   F   ++D Y+ +L+IWLSGA+TY++TCLD FEN TG
Sbjct: 130 SNCDELLKYAIDDLKESFDRI-GPFEYEKLDEYVDDLKIWLSGAITYEQTCLDGFENTTG 189

Query: 252 DTGEKMTNLLKTSKEMTANGLVMVGEVTSLVSSLWKALGLP-ETGRQLRAKELDEEDEEE 311
           + G +M  +L++S+E+T+NGL MV E++S+++S      LP    R+L + E   + ++ 
Sbjct: 190 EAGLRMKAMLRSSQELTSNGLAMVTEISSILASY----NLPFNVSRRLLSSE---DGDDT 249

Query: 312 EPSWVND-RRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGV 371
           E SW++D +R LLQATG  I+A+VVVAKDGSGKY+T+ +AL+D+P+K NKTFVIY+K+GV
Sbjct: 250 ELSWLSDGKRRLLQATGKTIKADVVVAKDGSGKYRTVNEALKDIPKKGNKTFVIYIKQGV 309

Query: 372 YQEQVMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFEN 431
           Y+EQVM  K+MT VM+IGDGPTKT+IT   NF DG  TFKTAT + +G+NFI KD+ FEN
Sbjct: 310 YKEQVMFAKSMTHVMIIGDGPTKTRITGSLNFIDGVQTFKTATVSVIGSNFIAKDIGFEN 369

Query: 432 SAGPEKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAV 491
           SAGP+KHQAVALRVQSDMSIFYNC MDGYQDTLY H HRQF+RDC ITGTIDFIFG++A 
Sbjct: 370 SAGPQKHQAVALRVQSDMSIFYNCHMDGYQDTLYAHTHRQFYRDCVITGTIDFIFGDSAA 429

Query: 492 ILQNCKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGR 551
           + QNCK+ +RKP++NQ CIVTAQGR ER+E T +VLQNC I+ DP + PV+   K YLGR
Sbjct: 430 VFQNCKMVIRKPLDNQNCIVTAQGRTERREATALVLQNCTITGDPAYLPVKDQNKGYLGR 489

Query: 552 PWKQYSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIK 611
           PWK++SRTIIM  +IDD+I PEGWLPW GDFAL T FYTE  NRGPG+    RVKWRGIK
Sbjct: 490 PWKEFSRTIIMNSRIDDVIAPEGWLPWAGDFALNTCFYTEILNRGPGSKLDGRVKWRGIK 549

Query: 612 QITPKHAIDFTPGLFIRGNPWIRRTGVPYYSGMATI 630
           ++  + A+++TPG FIRG+ WI+  GVPY SGM  +
Sbjct: 550 KVPAQRAVEYTPGRFIRGDWWIKAAGVPYVSGMLNV 577

BLAST of CmaCh06G015330 vs. NCBI nr
Match: gi|590661338|ref|XP_007035646.1| (Plant invertase/pectin methylesterase inhibitor superfamily, putative [Theobroma cacao])

HSP 1 Score: 656.8 bits (1693), Expect = 3.9e-185
Identity = 330/569 (58.00%), Postives = 425/569 (74.69%), Query Frame = 1

Query: 78  KVALVGLLSLFLVALV-----------------NGASGAGEDSGSSNVAIKTICEPTTYR 137
           KVA++ + S+FLVA+V                  GA+  GE S S+  AIK IC+PT YR
Sbjct: 125 KVAIISVCSVFLVAMVVAVAVGVSRSHGGRGNGGGATPTGEISTSTK-AIKAICQPTDYR 184

Query: 138 ETCEEALVKSNVDSKDPRELIKAGFHFAIEQLKAAIANSPTLKQAATDPMAKEAIDTCDE 197
           ETCE++L  S+ ++ DP+ELIK GF  AI ++K  IANS T+K+ A DPMA++A++ C E
Sbjct: 185 ETCEKSL--SSANTTDPKELIKIGFQAAISEIKKVIANSSTVKEVAKDPMARQALENCHE 244

Query: 198 LIDYAIDDLLTTFDQITDSFSLNRVDNYLKNLRIWLSGALTYQETCLDAFENVTGDTGEK 257
           L+DYAIDDL  +F+Q+ + F  +++D Y++NL+IWL GA+TYQ+TCLD F N TG+ G+K
Sbjct: 245 LMDYAIDDLKNSFNQLGE-FDFSKLDEYIENLKIWLGGAITYQQTCLDGFMNTTGEAGKK 304

Query: 258 MTNLLKTSKEMTANGLVMVGEVTSLVSSL-WKALGLPETG-RQLRAKELDEEDEEEEPSW 317
           M  LLKTS+E+T+NGL MV E++S++ +L  + +G   TG R+L A       E   P W
Sbjct: 305 MQALLKTSQELTSNGLAMVSEISSILGNLNIQNIGEISTGQRRLFA-------ENGFPMW 364

Query: 318 VN-DRRGLLQATGAKIRANVVVAKDGSGKYKTITQALQDVPRKSNKTFVIYVKEGVYQEQ 377
           V+ ++R  ++ T A I+ NVVVAKDGSGKY +I +ALQ+VP+ +  TFVIY+K GVY+EQ
Sbjct: 365 VSSEQRSRIRQTPATIKPNVVVAKDGSGKYTSINEALQEVPKDNPTTFVIYIKAGVYKEQ 424

Query: 378 VMVDKTMTWVMMIGDGPTKTKITAGKNFKDGTSTFKTATFAAVGTNFIGKDLWFENSAGP 437
           V+V K+MT VM IGDG TKTKIT   N+ DGT TF+TAT   VG  FI KD+ FEN+AG 
Sbjct: 425 VIVTKSMTNVMFIGDGRTKTKITGDLNYADGTGTFRTATVGVVGDGFIAKDIGFENTAGA 484

Query: 438 EKHQAVALRVQSDMSIFYNCRMDGYQDTLYTHAHRQFFRDCTITGTIDFIFGNAAVILQN 497
            KHQAVALRVQSD SIFYNC+MDGYQDTLY H+HRQF+RDCTI+GTIDFIFG++A +LQN
Sbjct: 485 IKHQAVALRVQSDQSIFYNCQMDGYQDTLYAHSHRQFYRDCTISGTIDFIFGDSATVLQN 544

Query: 498 CKIFVRKPMENQKCIVTAQGRLERKEPTGIVLQNCAISSDPGFFPVRHVFKTYLGRPWKQ 557
           CKI VRKP+ENQ+CIVTAQGR+ER+E + +VLQNC IS DP + PV+   K YLGRPWK+
Sbjct: 545 CKIIVRKPLENQQCIVTAQGRIERREVSALVLQNCTISGDPEYLPVKDKNKAYLGRPWKE 604

Query: 558 YSRTIIMQCQIDDLIQPEGWLPWEGDFALKTLFYTEFDNRGPGAAKQNRVKWRGIKQITP 617
           +SRT+IMQ QIDD+I PEGWLPW GDFAL TL+Y EF NRGPGA + +RV WRGIK+I  
Sbjct: 605 FSRTLIMQSQIDDIIAPEGWLPWNGDFALNTLWYAEFGNRGPGAVQTHRVTWRGIKKINA 664

Query: 618 KHAIDFTPGLFIRGNPWIRRTGVPYYSGM 627
             A  FTPG+F+RG+ WI + GVPY  G+
Sbjct: 665 AIARRFTPGVFLRGDDWIPKAGVPYTPGL 682