CmaCh06G009320 (gene) Cucurbita maxima (Rimu)

Overview
NameCmaCh06G009320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description3-oxo-5-alpha-steroid 4-dehydrogenase
LocationCma_Chr06 : 6302208 .. 6303796 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGATGGGTAGCGGCAGAAATGGCGGTAATCTGAAAAATGCTGTTGTAGCCTTACTGGTTCCGCTTCCCTCCATTTTCTTTTACCTCTGTTTCCTCAGCAACTGTGGTAATGATACTTGTACTGGTCTCTGGAAGTGGTGCTATCACCATCCTCTTCTATTGGTGAATGCACTCTTCTTCCTCAACGTCAACTTCGTCTTCTGGCTCCTCAGCCATGTACAATCCAGCCACTGGGTGAGCCTCCTTCCCCCAAAATTCTTCTCTTCTCGATTTCCTCAGCACCAAACGGCTGATTTCTATTTCATGTTTTGATTGGTGCAGATGATTGACCTGTACTGGACTTTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCACTATAATGAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATATGGAGCATAAGGCTTACCCACAACTACTTGAGGCGGGAGGGATGGCAGCTGGGAGTCAGAGAGGACTGGAGGTTCACTGATATGCGCCAACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTCTATTTTTCACAGCAGGTAAGAACTGATCTAATCCACGATACTCTCCTTGTCTATGCCTAAGCCATTTGGTTCATTTGTGTTTGTTTCCCTTCAACTTTAATACAGATATTCCTGGTAGGCGTATGCCTTCCTCTATACGTCGTCCACTCAGTTGACGAGGCATTGAATTTGTGGGATTTGGTAGCAATCTTTGTGTGTGTGTCTGGAATTGTGATCGCCTACTTTGCTGACACCCAACTCCACGAATTCGTGACAAGGAACAACGAGCTAAAAATGAGTGGAAAATCAATGGTGCCCAATTTAGAGGAGGGATTGTGGCGTTACTCCCGACACCCAAACTACTTTGGAGAGCAGCTGTGGTGGTGGGGACTTGTGATATTTGCGTGGGGTTTAGGTGAGGAATGGAGCCTTGTTGGATCAATGGTGAACAGTTTGTGCTTAGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGAGTACAGAGCTGAAGCTTACAGGCAATATCAGAAGACTACATCTGTGTGGATACCTTGGTTCAACACATCAGTGAAAGATGATGAAAAGGATAAGAATATGTGAGTAAATAAGCGTGGCTCTTTCTTTCTGTCAATGTGCAGCGTCTTCTCTTTTTATGAATTTGTATGCGGTCAGCTCAGCTTTGTTGGTTTATGGTAATCTTGTTGTAAAGCTTTCGCCTTTCTACTATTGGGTGAGAAAAAGTTGCAAGGATTGTTCTATCCTCTGTCTATGTGTAAGACACCCGCTCTTATCTTGTTTTCTTGGATTGGATCTCTTTCTCTCTCTCTTTCTCTTTAACACTCTTATGTTAAAAATGGAACCTAAGTAGTATTTCTTACAAATGGAATAAGTAGGAGTCCTCTAATAAAATACATTATTTATTCGACACTTAGTCACTTTTGACTACACCTTCGATGCTCATAACGTCTTTGTTCGACACTCGAGGATTCCATTAACATGACTAAGCTAAGAGCATGACCCTAATAACA

mRNA sequence

ATGGCGATGGGTAGCGGCAGAAATGGCGGTAATCTGAAAAATGCTGTTGTAGCCTTACTGGTTCCGCTTCCCTCCATTTTCTTTTACCTCTGTTTCCTCAGCAACTGTGGTAATGATACTTGTACTGGTCTCTGGAAGTGGTGCTATCACCATCCTCTTCTATTGGTGAATGCACTCTTCTTCCTCAACGTCAACTTCGTCTTCTGGCTCCTCAGCCATGTACAATCCAGCCACTGGATGATTGACCTGTACTGGACTTTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCACTATAATGAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATATGGAGCATAAGGCTTACCCACAACTACTTGAGGCGGGAGGGATGGCAGCTGGGAGTCAGAGAGGACTGGAGGTTCACTGATATGCGCCAACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTCTATTTTTCACAGCAGATATTCCTGGTAGGCGTATGCCTTCCTCTATACGTCGTCCACTCAGTTGACGAGGCATTGAATTTGTGGGATTTGGTAGCAATCTTTGTGTGTGTGTCTGGAATTGTGATCGCCTACTTTGCTGACACCCAACTCCACGAATTCGTGACAAGGAACAACGAGCTAAAAATGAGTGGAAAATCAATGGTGCCCAATTTAGAGGAGGGATTGTGGCGTTACTCCCGACACCCAAACTACTTTGGAGAGCAGCTGTGGTGGTGGGGACTTGTGATATTTGCGTGGGGTTTAGGTGAGGAATGGAGCCTTGTTGGATCAATGGTGAACAGTTTGTGCTTAGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGAGTACAGAGCTGAAGCTTACAGGCAATATCAGAAGACTACATCTGTGTGGATACCTTGGTTCAACACATCAGTGAAAGATGATGAAAAGGATAAGAATATGTGAGTAAATAAGCGTGGCTCTTTCTTTCTGTCAATGTGCAGCGTCTTCTCTTTTTATGAATTTGTATGCGGTCAGCTCAGCTTTGTTGGTTTATGGTAATCTTGTTGTAAAGCTTTCGCCTTTCTACTATTGGGTGAGAAAAAGTTGCAAGGATTGTTCTATCCTCTGTCTATGTGTAAGACACCCGCTCTTATCTTGTTTTCTTGGATTGGATCTCTTTCTCTCTCTCTTTCTCTTTAACACTCTTATGTTAAAAATGGAACCTAAGTAGTATTTCTTACAAATGGAATAAGTAGGAGTCCTCTAATAAAATACATTATTTATTCGACACTTAGTCACTTTTGACTACACCTTCGATGCTCATAACGTCTTTGTTCGACACTCGAGGATTCCATTAACATGACTAAGCTAAGAGCATGACCCTAATAACA

Coding sequence (CDS)

ATGGCGATGGGTAGCGGCAGAAATGGCGGTAATCTGAAAAATGCTGTTGTAGCCTTACTGGTTCCGCTTCCCTCCATTTTCTTTTACCTCTGTTTCCTCAGCAACTGTGGTAATGATACTTGTACTGGTCTCTGGAAGTGGTGCTATCACCATCCTCTTCTATTGGTGAATGCACTCTTCTTCCTCAACGTCAACTTCGTCTTCTGGCTCCTCAGCCATGTACAATCCAGCCACTGGATGATTGACCTGTACTGGACTTTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCACTATAATGAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATATGGAGCATAAGGCTTACCCACAACTACTTGAGGCGGGAGGGATGGCAGCTGGGAGTCAGAGAGGACTGGAGGTTCACTGATATGCGCCAACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTCTATTTTTCACAGCAGATATTCCTGGTAGGCGTATGCCTTCCTCTATACGTCGTCCACTCAGTTGACGAGGCATTGAATTTGTGGGATTTGGTAGCAATCTTTGTGTGTGTGTCTGGAATTGTGATCGCCTACTTTGCTGACACCCAACTCCACGAATTCGTGACAAGGAACAACGAGCTAAAAATGAGTGGAAAATCAATGGTGCCCAATTTAGAGGAGGGATTGTGGCGTTACTCCCGACACCCAAACTACTTTGGAGAGCAGCTGTGGTGGTGGGGACTTGTGATATTTGCGTGGGGTTTAGGTGAGGAATGGAGCCTTGTTGGATCAATGGTGAACAGTTTGTGCTTAGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGAGTACAGAGCTGAAGCTTACAGGCAATATCAGAAGACTACATCTGTGTGGATACCTTGGTTCAACACATCAGTGAAAGATGATGAAAAGGATAAGAATATGTGA

Protein sequence

MAMGSGRNGGNLKNAVVALLVPLPSIFFYLCFLSNCGNDTCTGLWKWCYHHPLLLVNALFFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVKDDEKDKNM
Homology
[Close]
BLAST of CmaCh06G009320 vs. TrEMBL
Match: A0A0A0L2V4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116670 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 4.0e-164
Identity = 270/327 (82.57%), Postives = 291/327 (88.99%), Query Frame = 1 

Query: 1   MAMGSGRNGGNLKNAVVALLVPLPSIFFYLCFLSNCGNDTCTGLWKWCYHHPLLLVNALF 60
           MA GS  NG NLKN V+ALLV LPSI FYLCFLSNCG    +GLWKWCYHHPLLLVNALF
Sbjct: 1   MAKGSSSNGNNLKNGVIALLVLLPSILFYLCFLSNCGGGCSSGLWKWCYHHPLLLVNALF 60

Query: 61  FLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWS 120
           FLNVN +FWL+SHVQSSHWMIDLYWTLIPVMLVHYFASHPLA YNELRSW+ +ALTWIWS
Sbjct: 61  FLNVNLLFWLISHVQSSHWMIDLYWTLIPVMLVHYFASHPLAEYNELRSWVVVALTWIWS 120

Query: 121 IRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVV 180
           IRL+HNYLRREGWQLG REDWRFTDMRQQYGKNWWWVSFFAVY SQQ+FL+GVC+PLYVV
Sbjct: 121 IRLSHNYLRREGWQLGTREDWRFTDMRQQYGKNWWWVSFFAVYLSQQVFLMGVCVPLYVV 180

Query: 181 HSVDEALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRY 240
           HSV E L LWDLVAIF+CVSGI +AYFADTQLHEFV+RN +LKMSGK+MVPNLEEGLWRY
Sbjct: 181 HSVKEELKLWDLVAIFICVSGIGMAYFADTQLHEFVSRNRKLKMSGKAMVPNLEEGLWRY 240

Query: 241 SRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAY 300
            RHPNYFGEQLWWWG+ I  WGLG  WSLVGS++N++CLAYVTKLVE RMVKQ YRAEAY
Sbjct: 241 CRHPNYFGEQLWWWGVGILGWGLGVGWSLVGSLLNTMCLAYVTKLVEARMVKQHYRAEAY 300

Query: 301 RQYQKTTSVWIPWFNTSVKDDEKDKNM 328
           RQYQKTTSVWIPWF  S+    K KNM
Sbjct: 301 RQYQKTTSVWIPWFRKSL---YKQKNM 324

[Close]
BLAST of CmaCh06G009320 vs. TrEMBL
Match: M5X311_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008700mg PE=4 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-142
Identity = 231/321 (71.96%), Postives = 270/321 (84.11%), Query Frame = 1 

Query: 9   GGNLKNAVVALLVPLPSIFFYLCFLSNCGNDT---CTGLWKWCYHHPLLLVNALFFLNVN 68
           GGNLKNAVVA L PLPSI FYL FLSN  + T    + LW WCYHHPLLL NALFFLNVN
Sbjct: 3   GGNLKNAVVAFLAPLPSIIFYLIFLSNNHHQTDGILSPLWAWCYHHPLLLANALFFLNVN 62

Query: 69  FVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTH 128
            +FW++S V  SHWMIDLYWT+IPVMLVHY+ SHPLA YN  RS I + +TW+WS+RL+H
Sbjct: 63  VLFWVISQVLKSHWMIDLYWTVIPVMLVHYYGSHPLADYNWWRSTIVVLMTWVWSLRLSH 122

Query: 129 NYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDE 188
           NY RRE WQ G REDWRFTDM  QYGK+WWW+SFFAVY SQQ+FL+GVCLP+YV+HSV+ 
Sbjct: 123 NYFRRENWQWGAREDWRFTDMSSQYGKHWWWISFFAVYLSQQVFLIGVCLPMYVIHSVNV 182

Query: 189 ALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPN 248
            LN+WD++A+ VC+SGIV+AY ADTQLH+FV+RNN+LK  GK +VPNL+EGLWRYSRHPN
Sbjct: 183 PLNIWDIIAVIVCISGIVVAYCADTQLHDFVSRNNKLKELGKPVVPNLDEGLWRYSRHPN 242

Query: 249 YFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQK 308
           YFGEQLWWWGLVIFAW LG  W+ VGS++N++CLAYVT LVE RM+KQ+YRAEAYR+YQK
Sbjct: 243 YFGEQLWWWGLVIFAWSLGHGWAFVGSLINTMCLAYVTTLVERRMLKQDYRAEAYRRYQK 302

Query: 309 TTSVWIPWFNTSVKDDEKDKN 327
           TTSVWIPWF +S     KDKN
Sbjct: 303 TTSVWIPWFKSS--PGGKDKN 321

[Close]
BLAST of CmaCh06G009320 vs. TrEMBL
Match: A0A0B0NW86_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_06353 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 1.5e-139
Identity = 227/318 (71.38%), Postives = 264/318 (83.02%), Query Frame = 1 

Query: 10  GNLKNAVVALLVPLPSIFFYLCFLSN--CGNDTCTGLWKWCYHHPLLLVNALFFLNVNFV 69
           GNLKNA+VA LVPLPSI FYL FL +    + + + LW WC+HHPLLL N  FF NVN +
Sbjct: 5   GNLKNAIVAFLVPLPSILFYLYFLDHFHSNSSSLSSLWSWCFHHPLLLANVFFFFNVNVL 64

Query: 70  FWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNY 129
           FW + H+QSS WMIDLYWT+IPV+L+HY+A+HP A ++ LRS I I LTW+WSIRLTHNY
Sbjct: 65  FWTIGHIQSSDWMIDLYWTVIPVLLIHYYATHPSAQFDGLRSKIVIVLTWVWSIRLTHNY 124

Query: 130 LRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEAL 189
            RRE WQ G REDWRF DMR QYGK+WWW+SFF+VYFSQQIFL G+CLPLYVVHSVDE +
Sbjct: 125 FRREKWQWGAREDWRFADMRLQYGKHWWWISFFSVYFSQQIFLFGICLPLYVVHSVDEPM 184

Query: 190 NLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYF 249
           N+WDLVA  VC+ GIVIAYFADTQLH FVTRN +LK  GK MVPNL+ GLW+YSRHPNYF
Sbjct: 185 NIWDLVAAGVCLCGIVIAYFADTQLHNFVTRNTKLKELGKPMVPNLDRGLWQYSRHPNYF 244

Query: 250 GEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTT 309
           GEQLWWWGLV+FAW LG  W+ VGS++NS+CLAYVT LVE+RM+KQEYRAEAYR YQKTT
Sbjct: 245 GEQLWWWGLVVFAWNLGHGWTFVGSLINSMCLAYVTVLVEQRMLKQEYRAEAYRLYQKTT 304

Query: 310 SVWIPWFNTS---VKDDE 323
           SVW+PWF +S   VKD +
Sbjct: 305 SVWVPWFRSSAIAVKDKD 322

[Close]
BLAST of CmaCh06G009320 vs. TrEMBL
Match: A0A0D2SBM9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G114800 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 4.5e-139
Identity = 224/318 (70.44%), Postives = 266/318 (83.65%), Query Frame = 1 

Query: 10  GNLKNAVVALLVPLPSIFFYLCFLSN--CGNDTCTGLWKWCYHHPLLLVNALFFLNVNFV 69
           GNLKNA+VA LVPLPSI FYL FL +    + + + LW WC+HHPLLL N  FF NVN +
Sbjct: 5   GNLKNAIVAFLVPLPSILFYLYFLDHFHSNSSSLSSLWSWCFHHPLLLANVFFFFNVNVL 64

Query: 70  FWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNY 129
           FW++ H+QSS+WMIDLYWT+IPV+L+HY+A+HP A ++ LRS I I LTW+WSIRLTH+Y
Sbjct: 65  FWIIGHIQSSNWMIDLYWTVIPVLLIHYYATHPSAQFDGLRSKIVIVLTWVWSIRLTHSY 124

Query: 130 LRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEAL 189
            RRE WQ G REDWRF DMR QYGK+WWW+SFF+VYFSQQIFL+G+CLPLYVVHSVD+ +
Sbjct: 125 FRREKWQWGAREDWRFADMRLQYGKHWWWISFFSVYFSQQIFLIGICLPLYVVHSVDKPM 184

Query: 190 NLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYF 249
           N+WDLVA  VC+ GIVIAYFADTQLH FVTRN +LK  GK MVPNL+ GLW+YSRHPNYF
Sbjct: 185 NIWDLVAAGVCLCGIVIAYFADTQLHNFVTRNTKLKELGKPMVPNLDRGLWQYSRHPNYF 244

Query: 250 GEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTT 309
           GEQLWWWGLV+FAW LG  W+ VGS++NS+C AYVT LVE+RM+KQEYRAEAYR YQKTT
Sbjct: 245 GEQLWWWGLVVFAWNLGHGWTFVGSLINSMCFAYVTVLVEQRMLKQEYRAEAYRLYQKTT 304

Query: 310 SVWIPWFNTS---VKDDE 323
           SVW+PWF +S   VKD +
Sbjct: 305 SVWVPWFRSSAIAVKDKD 322

[Close]
BLAST of CmaCh06G009320 vs. TrEMBL
Match: A0A061DZR2_THECC (Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_004997 PE=4 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.8e-138
Identity = 228/309 (73.79%), Postives = 262/309 (84.79%), Query Frame = 1 

Query: 11  NLKNAVVALLVPLPSIFFYLCFLSNCGND--TCTGLWKWCYHHPLLLVNALFFLNVNFVF 70
           NLKNAVVA LVPLPSI FYL FLS+  +D  + + LW WC+HHPLLL NALFF NVN +F
Sbjct: 5   NLKNAVVAFLVPLPSILFYLHFLSHFQSDRNSLSPLWSWCFHHPLLLANALFFFNVNVLF 64

Query: 71  WLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYL 130
           WL+SH+QSSHWMIDLYWT+IPV+LVH++A+HPLA ++  RS I I LTW+WSIRLTHNY 
Sbjct: 65  WLISHIQSSHWMIDLYWTVIPVLLVHHYATHPLAQFDWWRSKIVIVLTWVWSIRLTHNYF 124

Query: 131 RREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEALN 190
           RRE W  G REDWRFTDM QQYGK+WWW+SFFAVYFSQQIFL+G+CLPL VVHSVD  LN
Sbjct: 125 RREKWHWGAREDWRFTDMSQQYGKHWWWISFFAVYFSQQIFLIGICLPLSVVHSVDMPLN 184

Query: 191 LWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFG 250
           +WD VA  VC+ GIVIAYFADTQLH FVTRN++LK  GK MVPNL+ GLW+YSRHPNYFG
Sbjct: 185 MWDFVAAGVCLCGIVIAYFADTQLHNFVTRNSKLKELGKPMVPNLDRGLWQYSRHPNYFG 244

Query: 251 EQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTS 310
           EQLWWWGLVIFAW LG  W+ VGS++NS+CLAYVT LVE RM+K+EYRAEAYR YQKTTS
Sbjct: 245 EQLWWWGLVIFAWNLGHGWTFVGSLINSMCLAYVTVLVERRMLKKEYRAEAYRLYQKTTS 304

Query: 311 VWIPWFNTS 318
             +PWF +S
Sbjct: 305 ACVPWFRSS 313

[Close]
BLAST of CmaCh06G009320 vs. TAIR10
Match: AT2G46890.1 (AT2G46890.1 Protein of unknown function (DUF1295))

HSP 1 Score: 491.9 bits (1265), Expect = 3.1e-139
Identity = 221/319 (69.28%), Postives = 264/319 (82.76%), Query Frame = 1 

Query: 11  NLKNAVVALLVPLPSIFFYLCFLSNCGNDT---CTGLWKWCYHHPLLLVNALFFLNVNFV 70
           NL+NA+VA L PLPSI FYL FLSN  + +    + +  WC +HPLLL N LFFLNVN +
Sbjct: 4   NLRNAIVAFLAPLPSIVFYLTFLSNYSSSSDSELSSIQSWCLNHPLLLANLLFFLNVNVL 63

Query: 71  FWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNY 130
           FW++  +QSSHWMID+YWT+IPVMLVHYFASHPL+ YN+LRS I I LTWIWSIRLTHNY
Sbjct: 64  FWVIGLLQSSHWMIDVYWTVIPVMLVHYFASHPLSQYNKLRSMIVITLTWIWSIRLTHNY 123

Query: 131 LRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEAL 190
            RRE W+ G REDWRF D+R+QYGK+WWW+SFF+VY SQQIFL+G+CLPLYV+HS+DE L
Sbjct: 124 FRRENWEWGAREDWRFNDLRKQYGKHWWWLSFFSVYVSQQIFLIGICLPLYVIHSIDEPL 183

Query: 191 NLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYF 250
           N+WD ++  +C++GIV+AYFADTQLHEFVT N +LK  GK  +PNL+ GLWRYSRHPNY 
Sbjct: 184 NIWDFISSAICLTGIVMAYFADTQLHEFVTGNQKLKEQGKPKIPNLDSGLWRYSRHPNYL 243

Query: 251 GEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTT 310
           GEQLWWWGLVIFAW LG+ W+L+G++VN+LCL YVT LVE RMVKQ+YRAEAYR YQKTT
Sbjct: 244 GEQLWWWGLVIFAWNLGQGWTLIGALVNTLCLVYVTILVERRMVKQQYRAEAYRAYQKTT 303

Query: 311 SVWIPWFNTSVKDDEKDKN 327
           SVWIPWF +      KDKN
Sbjct: 304 SVWIPWFKSHAA-ATKDKN 321

[Close]
BLAST of CmaCh06G009320 vs. TAIR10
Match: AT1G18180.1 (AT1G18180.1 Protein of unknown function (DUF1295))

HSP 1 Score: 73.9 bits (180), Expect = 2.0e-13
Identity = 77/268 (28.73%), Postives = 123/268 (45.90%), Query Frame = 1 

Query: 54  LLVNALFFLNVNFVFWLLSHVQSSHWMIDLYW-TLIPVMLVHYFASHPLAHYNELRSWIA 113
           L + A+  +   F+F++++ +     + D    T   ++ V         HY ++   + 
Sbjct: 10  LAITAIVTVGYQFIFFVITALFKFDQVTDFAGSTNFVILAVLTLLLKGTWHYRQI---VL 69

Query: 114 IALTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVG 173
             L  +W +RL    L R    L   ED RF DMR+  GK          +  Q +++  
Sbjct: 70  TVLVVVWGLRLGLFLLMRI---LQWGEDRRFDDMRENIGK------LVVFWIFQAVWVWT 129

Query: 174 VCLPLYVVHSVD--EALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMV 233
           V LP+  V++ +        D++   + V+G +I   AD Q           K+S K   
Sbjct: 130 VSLPVTFVNASNGGRLFQPADVIGWTMWVAGFLIEATADQQ-----------KLSFKKCP 189

Query: 234 PN----LEEGLWRYSRHPNYFGEQLWWWGLVIFAW----GLGEEWSLVGSMVNSLCLAYV 293
            N     + G+W+YSRHPNYFGE L WWGL + +     GL E   ++G +  +L L +V
Sbjct: 190 ENKGKWCDVGVWKYSRHPNYFGEMLLWWGLYVASLPVLKGL-EYLVIIGPVFLTLLLLFV 249

Query: 294 T--KLVEERMVKQEYRAEAYRQYQKTTS 309
           +   L+EE   K+     AYR Y+KTTS
Sbjct: 250 SGIPLLEESADKKYGNLGAYRHYKKTTS 253

[Close]
BLAST of CmaCh06G009320 vs. TAIR10
Match: AT1G73650.3 (AT1G73650.3 Protein of unknown function (DUF1295))

HSP 1 Score: 71.2 bits (173), Expect = 1.3e-12
Identity = 67/208 (32.21%), Postives = 101/208 (48.56%), Query Frame = 1 

Query: 108 RSWIAIALTWIWSIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQ 167
           R  +   L  +W +RL    L R    L   ED RF + R         V     +  Q 
Sbjct: 62  RQIVLTLLVVVWGLRLGIFLLMRI---LQWGEDRRFDEQRGNI------VRLIIFWTLQA 121

Query: 168 IFLVGVCLPLYVVHSVDE--ALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMS 227
           +++  V LPL +V++ D   +L   D++   + V G +I   AD Q   F    N  +  
Sbjct: 122 VWVWTVSLPLTLVNASDGGGSLKPADVIGWTMWVFGFLIEAAADQQKLSF---KNSPENR 181

Query: 228 GKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIFAWGL--GEEWSLV-GSMVNSLCLAYV 287
           GK      + G+W+YSRHPNYFGE L WWG+ + A  +  G E+ ++ G +  +L L +V
Sbjct: 182 GKWC----DVGVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFV 241

Query: 288 T--KLVEERMVKQEYRAEAYRQYQKTTS 309
           +   L+E    K+   + AYR Y+KTTS
Sbjct: 242 SGIPLLEASADKKHGNSGAYRSYKKTTS 253

[Close]
BLAST of CmaCh06G009320 vs. NCBI nr
Match: gi|449432978|ref|XP_004134275.1| (PREDICTED: uncharacterized protein C594.04c [Cucumis sativus])

HSP 1 Score: 585.5 bits (1508), Expect = 5.8e-164
Identity = 270/327 (82.57%), Postives = 291/327 (88.99%), Query Frame = 1 

Query: 1   MAMGSGRNGGNLKNAVVALLVPLPSIFFYLCFLSNCGNDTCTGLWKWCYHHPLLLVNALF 60
           MA GS  NG NLKN V+ALLV LPSI FYLCFLSNCG    +GLWKWCYHHPLLLVNALF
Sbjct: 1   MAKGSSSNGNNLKNGVIALLVLLPSILFYLCFLSNCGGGCSSGLWKWCYHHPLLLVNALF 60

Query: 61  FLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWS 120
           FLNVN +FWL+SHVQSSHWMIDLYWTLIPVMLVHYFASHPLA YNELRSW+ +ALTWIWS
Sbjct: 61  FLNVNLLFWLISHVQSSHWMIDLYWTLIPVMLVHYFASHPLAEYNELRSWVVVALTWIWS 120

Query: 121 IRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVV 180
           IRL+HNYLRREGWQLG REDWRFTDMRQQYGKNWWWVSFFAVY SQQ+FL+GVC+PLYVV
Sbjct: 121 IRLSHNYLRREGWQLGTREDWRFTDMRQQYGKNWWWVSFFAVYLSQQVFLMGVCVPLYVV 180

Query: 181 HSVDEALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRY 240
           HSV E L LWDLVAIF+CVSGI +AYFADTQLHEFV+RN +LKMSGK+MVPNLEEGLWRY
Sbjct: 181 HSVKEELKLWDLVAIFICVSGIGMAYFADTQLHEFVSRNRKLKMSGKAMVPNLEEGLWRY 240

Query: 241 SRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAY 300
            RHPNYFGEQLWWWG+ I  WGLG  WSLVGS++N++CLAYVTKLVE RMVKQ YRAEAY
Sbjct: 241 CRHPNYFGEQLWWWGVGILGWGLGVGWSLVGSLLNTMCLAYVTKLVEARMVKQHYRAEAY 300

Query: 301 RQYQKTTSVWIPWFNTSVKDDEKDKNM 328
           RQYQKTTSVWIPWF  S+    K KNM
Sbjct: 301 RQYQKTTSVWIPWFRKSL---YKQKNM 324

[Close]
BLAST of CmaCh06G009320 vs. NCBI nr
Match: gi|659074719|ref|XP_008437759.1| (PREDICTED: uncharacterized protein C594.04c [Cucumis melo])

HSP 1 Score: 570.5 bits (1469), Expect = 1.9e-159
Identity = 265/328 (80.79%), Postives = 289/328 (88.11%), Query Frame = 1 

Query: 1   MAMGSGRNGGNLKNAVVALLVPLPSIFFYLCFLSNCGNDTC-TGLWKWCYHHPLLLVNAL 60
           MA GS  N  +LKN V+ALLVPLPSI FYLCFLSNCG   C +GLWKWCYHHPLLLVNAL
Sbjct: 1   MAKGSSSNVNSLKNGVIALLVPLPSILFYLCFLSNCGGGGCSSGLWKWCYHHPLLLVNAL 60

Query: 61  FFLNVNFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIW 120
           FFLNVN +FWL+S VQSSHWMIDLYWTLIPV+LVHYFASHPLA YNELRSW+ +ALTWIW
Sbjct: 61  FFLNVNLLFWLISLVQSSHWMIDLYWTLIPVVLVHYFASHPLAEYNELRSWVVVALTWIW 120

Query: 121 SIRLTHNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYV 180
           S RL+HNYLRREGWQLG REDWRFTDMRQQYG+NWWWVSFFAVY SQQ+FL+GVC+PLYV
Sbjct: 121 STRLSHNYLRREGWQLGAREDWRFTDMRQQYGRNWWWVSFFAVYLSQQVFLMGVCVPLYV 180

Query: 181 VHSVDEALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWR 240
           VHSV E L LWDLVAIF+CVSGI +AYFADTQLHEFV+RN +LKMSGK+MVPNLEEGLWR
Sbjct: 181 VHSVKEELKLWDLVAIFMCVSGIGMAYFADTQLHEFVSRNRKLKMSGKAMVPNLEEGLWR 240

Query: 241 YSRHPNYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEA 300
           Y RHPNYFGEQLWWWG+ I  WGLG  WS VGS++N++CLAYVTKLVE RMVKQ YRAEA
Sbjct: 241 YCRHPNYFGEQLWWWGVGILGWGLGVGWSPVGSLLNTMCLAYVTKLVEARMVKQHYRAEA 300

Query: 301 YRQYQKTTSVWIPWFNTSVKDDEKDKNM 328
           YRQYQKTTSVWIPWF  S+    K KNM
Sbjct: 301 YRQYQKTTSVWIPWFRKSL---YKQKNM 325

[Close]
BLAST of CmaCh06G009320 vs. NCBI nr
Match: gi|645278301|ref|XP_008244169.1| (PREDICTED: uncharacterized protein C594.04c [Prunus mume])

HSP 1 Score: 513.1 bits (1320), Expect = 3.7e-142
Identity = 231/321 (71.96%), Postives = 270/321 (84.11%), Query Frame = 1 

Query: 9   GGNLKNAVVALLVPLPSIFFYLCFLSNCGNDT---CTGLWKWCYHHPLLLVNALFFLNVN 68
           GGNLKNAVVA L PLPSI FYL FLSN  + T    + LW WCYHHPLLL NALFFLNVN
Sbjct: 11  GGNLKNAVVAFLAPLPSIIFYLIFLSNNHHQTDGILSPLWAWCYHHPLLLANALFFLNVN 70

Query: 69  FVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTH 128
            +FW++S V  +HWMIDLYWT+IPVMLVHY+ SHPLA YN  RS I + +TW+WS+RL+H
Sbjct: 71  VLFWVISQVLKNHWMIDLYWTVIPVMLVHYYGSHPLADYNWWRSTIVVLMTWVWSLRLSH 130

Query: 129 NYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDE 188
           NY RRE WQ G REDWRFTDM  QYGK+WWW+SFFAVY SQQ+FL+GVCLP+YV+HSV+ 
Sbjct: 131 NYFRRENWQWGAREDWRFTDMSSQYGKHWWWISFFAVYLSQQVFLIGVCLPMYVIHSVNV 190

Query: 189 ALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPN 248
            LN+WD+VA+ VC+SGIV+AY ADTQLH+FV+RNN+LK  GK +VPNL+EGLWRYSRHPN
Sbjct: 191 PLNIWDIVAVIVCISGIVVAYCADTQLHDFVSRNNKLKQLGKPVVPNLDEGLWRYSRHPN 250

Query: 249 YFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQK 308
           YFGEQLWWWGLVIFAW LG  W+ VGS++N++CLAYVT LVE RM+KQ+YRAEAYR+YQK
Sbjct: 251 YFGEQLWWWGLVIFAWSLGHGWAFVGSLINTMCLAYVTTLVERRMLKQDYRAEAYRRYQK 310

Query: 309 TTSVWIPWFNTSVKDDEKDKN 327
           TTSVWIPWF +S     KDKN
Sbjct: 311 TTSVWIPWFKSS--PGGKDKN 329

[Close]
BLAST of CmaCh06G009320 vs. NCBI nr
Match: gi|596012773|ref|XP_007218682.1| (hypothetical protein PRUPE_ppa008700mg [Prunus persica])

HSP 1 Score: 513.1 bits (1320), Expect = 3.7e-142
Identity = 231/321 (71.96%), Postives = 270/321 (84.11%), Query Frame = 1 

Query: 9   GGNLKNAVVALLVPLPSIFFYLCFLSNCGNDT---CTGLWKWCYHHPLLLVNALFFLNVN 68
           GGNLKNAVVA L PLPSI FYL FLSN  + T    + LW WCYHHPLLL NALFFLNVN
Sbjct: 3   GGNLKNAVVAFLAPLPSIIFYLIFLSNNHHQTDGILSPLWAWCYHHPLLLANALFFLNVN 62

Query: 69  FVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTH 128
            +FW++S V  SHWMIDLYWT+IPVMLVHY+ SHPLA YN  RS I + +TW+WS+RL+H
Sbjct: 63  VLFWVISQVLKSHWMIDLYWTVIPVMLVHYYGSHPLADYNWWRSTIVVLMTWVWSLRLSH 122

Query: 129 NYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDE 188
           NY RRE WQ G REDWRFTDM  QYGK+WWW+SFFAVY SQQ+FL+GVCLP+YV+HSV+ 
Sbjct: 123 NYFRRENWQWGAREDWRFTDMSSQYGKHWWWISFFAVYLSQQVFLIGVCLPMYVIHSVNV 182

Query: 189 ALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPN 248
            LN+WD++A+ VC+SGIV+AY ADTQLH+FV+RNN+LK  GK +VPNL+EGLWRYSRHPN
Sbjct: 183 PLNIWDIIAVIVCISGIVVAYCADTQLHDFVSRNNKLKELGKPVVPNLDEGLWRYSRHPN 242

Query: 249 YFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQK 308
           YFGEQLWWWGLVIFAW LG  W+ VGS++N++CLAYVT LVE RM+KQ+YRAEAYR+YQK
Sbjct: 243 YFGEQLWWWGLVIFAWSLGHGWAFVGSLINTMCLAYVTTLVERRMLKQDYRAEAYRRYQK 302

Query: 309 TTSVWIPWFNTSVKDDEKDKN 327
           TTSVWIPWF +S     KDKN
Sbjct: 303 TTSVWIPWFKSS--PGGKDKN 321

[Close]
BLAST of CmaCh06G009320 vs. NCBI nr
Match: gi|657969082|ref|XP_008376257.1| (PREDICTED: uncharacterized protein C594.04c [Malus domestica])

HSP 1 Score: 505.0 bits (1299), Expect = 1.0e-139
Identity = 229/322 (71.12%), Postives = 265/322 (82.30%), Query Frame = 1 

Query: 9   GGNLKNAVVALLVPLPSIFFYLCFLSNCGNDT----CTGLWKWCYHHPLLLVNALFFLNV 68
           GGNLKNAV+A L PLPSI FYL FLSN  + T     + LW WCYHHPLLL N  FFLNV
Sbjct: 3   GGNLKNAVIAFLAPLPSILFYLIFLSNHHSQTNESFLSPLWAWCYHHPLLLANVFFFLNV 62

Query: 69  NFVFWLLSHVQSSHWMIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLT 128
           N +FW++S   SSHWMIDLYWT+IPVMLVHY+  HPLA YN  RS IAI+LTW+WS+RL+
Sbjct: 63  NVLFWVISQALSSHWMIDLYWTVIPVMLVHYYRCHPLADYNRWRSTIAISLTWVWSLRLS 122

Query: 129 HNYLRREGWQLGVREDWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVD 188
           HNY RRE WQ G REDWRFTDM  QYGK+WWWVSFFAVY SQQ+F++G+CLP YV+HSV+
Sbjct: 123 HNYFRRENWQWGAREDWRFTDMSSQYGKHWWWVSFFAVYLSQQVFIIGICLPFYVIHSVN 182

Query: 189 EALNLWDLVAIFVCVSGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHP 248
             LN+WD VAI VC++GIV+AY ADTQLH+FV+RNN+LK  GK +VPNL+EGLW YSRHP
Sbjct: 183 VPLNIWDSVAIIVCITGIVVAYLADTQLHDFVSRNNKLKELGKPVVPNLDEGLWHYSRHP 242

Query: 249 NYFGEQLWWWGLVIFAWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQ 308
           NYFGEQLWWWGLVIFAW LG  W+ VGS+VN++CLAYVT LVE RM+KQ+YR EAYR+YQ
Sbjct: 243 NYFGEQLWWWGLVIFAWNLGHGWAFVGSLVNTMCLAYVTILVERRMLKQDYRVEAYRRYQ 302

Query: 309 KTTSVWIPWFNTSVKDDEKDKN 327
           KTTSVWIPWF +S     KDKN
Sbjct: 303 KTTSVWIPWFKSS--PGGKDKN 322

The following BLAST results are available for this feature:
BLAST of CmaCh06G009320 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BLAST of CmaCh06G009320 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L2V4_CUCSA4.0e-16482.57Uncharacterized protein OS=Cucumis sativus GN=Csa_3G116670 PE=4 SV=1[more]
M5X311_PRUPE2.5e-14271.96Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008700mg PE=4 SV=1[more]
A0A0B0NW86_GOSAR1.5e-13971.38Uncharacterized protein OS=Gossypium arboreum GN=F383_06353 PE=4 SV=1[more]
A0A0D2SBM9_GOSRA4.5e-13970.44Uncharacterized protein OS=Gossypium raimondii GN=B456_007G114800 PE=4 SV=1[more]
A0A061DZR2_THECC3.8e-13873.79Uncharacterized protein isoform 1 OS=Theobroma cacao GN=TCM_004997 PE=4 SV=1[more]
BLAST of CmaCh06G009320 vs. TAIR10
Match NameE-valueIdentityDescription
AT2G46890.13.1e-13969.28 Protein of unknown function (DUF1295)[more]
AT1G18180.12.0e-1328.73 Protein of unknown function (DUF1295)[more]
AT1G73650.31.3e-1232.21 Protein of unknown function (DUF1295)[more]
BLAST of CmaCh06G009320 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449432978|ref|XP_004134275.1|5.8e-16482.57PREDICTED: uncharacterized protein C594.04c [Cucumis sativus][more]
gi|659074719|ref|XP_008437759.1|1.9e-15980.79PREDICTED: uncharacterized protein C594.04c [Cucumis melo][more]
gi|645278301|ref|XP_008244169.1|3.7e-14271.96PREDICTED: uncharacterized protein C594.04c [Prunus mume][more]
gi|596012773|ref|XP_007218682.1|3.7e-14271.96hypothetical protein PRUPE_ppa008700mg [Prunus persica][more]
gi|657969082|ref|XP_008376257.1|1.0e-13971.12PREDICTED: uncharacterized protein C594.04c [Malus domestica][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0011043-oxo-5_a-steroid_4-DH_C
IPR010721DUF1295
Vocabulary: Molecular Function
TermDefinition
GO:0016627oxidoreductase activity, acting on the CH-CH group of donors
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
GO:0016021integral component of membrane
Vocabulary: Biological Process
TermDefinition
GO:0006629lipid metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh06G009320.1CmaCh06G009320.1mRNA


InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROFILEPS50244S5A_REDUCTASEcoord: 194..263
score: 10
IPR010721Protein of unknown function DUF1295PFAMPF06966DUF1295coord: 68..307
score: 4.7
NoneNo IPR availablePANTHERPTHR32251FAMILY NOT NAMEDcoord: 30..326
score: 2.4E
NoneNo IPR availablePANTHERPTHR32251:SF14SUBFAMILY NOT NAMEDcoord: 30..326
score: 2.4E