CmaCh06G007130 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh06G007130
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Terpene synthase
Location	Cma_Chr06 : 3579709 .. 3580280 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTGTCCCTCGTCGATTTGCAAACTTTCAACCAAGTGCTTGGGGGATTACTTCATTTCCTATGATTCCAACACTATGGTATATATATATATATCTTCGAATATTGTTCTTTAAATTACTAATTTTGATTCAATCTTTCGTGTTTATTTCATTCGGATTCCTACAATGATATCGTTTATGTTTGTACAAATATTAAGGAAAATCAGTTAGAAGAGAAGATGGTTGAGAAACAGCTTCAAAAGCTAAAAGAAGACGTGATGAACATGTTTGTGACAGCGGATAAGTGTTCGGAAAAATTAAGGTTGATAGATTTAATTCAACGCTTAGGTTTGTCCCATCACTTTGAACTGCAAATTAATGAAACACTTCAACTATTACAAAGCTCTTCCGAAGTTGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGTTTATTCTATTTCATCGGGTAAGTCTCTAGCTCCACCATCTTTTGTGAAGGACAAACGTGGAATATTAAGTCTGTACGAGGCATCCCATTTGGGAATGAAGGGAGAGTTGGCCAATCTATAA

mRNA sequence

ATGGGTTGTCCCTCGTCGATTTGCAAACTTTCAACCAAGTGCTTGGGGGATTACTTCATTTCCTATGATTCCAACACTATGGAAAATCAGTTAGAAGAGAAGATGGTTGAGAAACAGCTTCAAAAGCTAAAAGAAGACGTGATGAACATGTTTGTGACAGCGGATAAGTGTTCGGAAAAATTAAGGTTGATAGATTTAATTCAACGCTTAGGTTTGTCCCATCACTTTGAACTGCAAATTAATGAAACACTTCAACTATTACAAAGCTCTTCCGAAGTTGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGTTTATTCTATTTCATCGGGTAAGTCTCTAGCTCCACCATCTTTTGTGAAGGACAAACGTGGAATATTAAGTCTGTACGAGGCATCCCATTTGGGAATGAAGGGAGAGTTGGCCAATCTATAA

Coding sequence (CDS)

ATGGGTTGTCCCTCGTCGATTTGCAAACTTTCAACCAAGTGCTTGGGGGATTACTTCATTTCCTATGATTCCAACACTATGGAAAATCAGTTAGAAGAGAAGATGGTTGAGAAACAGCTTCAAAAGCTAAAAGAAGACGTGATGAACATGTTTGTGACAGCGGATAAGTGTTCGGAAAAATTAAGGTTGATAGATTTAATTCAACGCTTAGGTTTGTCCCATCACTTTGAACTGCAAATTAATGAAACACTTCAACTATTACAAAGCTCTTCCGAAGTTGATGACGATATTTACAATATTGCCCTTAAGTTTCGATTGCTTAGACAAGAAGTTTATTCTATTTCATCGGGTAAGTCTCTAGCTCCACCATCTTTTGTGAAGGACAAACGTGGAATATTAAGTCTGTACGAGGCATCCCATTTGGGAATGAAGGGAGAGTTGGCCAATCTATAA

Protein sequence

MGCPSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHHFELQINETLQLLQSSSEVDDDIYNIALKFRLLRQEVYSISSGKSLAPPSFVKDKRGILSLYEASHLGMKGELANL

Homology

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BLAST of CmaCh06G007130 vs. Swiss-Prot
Match: AFSY1_CUCME (Alpha-farnesene synthase OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 2.1e-20
Identity = 66/145 (45.52%), Postives = 90/145 (62.07%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMF--VTADKCSEKLRLIDLIQRLGLS 75
           GDYF+SY S + E     K++EKQ ++LKE++       T D+  EKLRLID IQRLG+ 
Sbjct: 27  GDYFLSYHSLSPEKG--NKVMEKQTEELKEEIKRELNSTTKDEEPEKLRLIDSIQRLGVC 86

Query: 76  HHFELQINETLQLLQ-----SSSEVDDDIYNIALKFRLLRQEVYSISSG-----KSLAPP 135
           +HFE +IN+ L+ L      S +  DD  YN+ L+FRLLRQ+ Y+ISS      +     
Sbjct: 87  YHFEYEINKILEQLHHITITSKNNGDDHPYNMTLRFRLLRQQGYNISSKSFERFRGKWES 146

Query: 136 SFVKDKRGILSLYEASHLGMKGELA 149
           S+ K+   +LSLYEAS L M+GE A
Sbjct: 147 SYDKNVEELLSLYEASQLRMRGEEA 169

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BLAST of CmaCh06G007130 vs. Swiss-Prot
Match: TPS3_RICCO (Probable terpene synthase 3 OS=Ricinus communis GN=TPS3 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.1e-19
Identity = 59/143 (41.26%), Postives = 93/143 (65.03%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMV---EKQLQKLKEDVMNMFVT-ADKCSEKLRLIDLIQRLG 75
           GDYFI       +N  + KMV   ++Q + L+E+V  M ++   K S KL+LID+IQRLG
Sbjct: 26  GDYFI-------KNAPDNKMVSIWKEQAKVLEEEVRRMIISETQKPSGKLKLIDVIQRLG 85

Query: 76  LSHHFELQINETLQLLQSSSEVDDDIYNIALKFRLLRQEVYSISSG--------KSLAPP 135
           +++HFE +I E ++ + S+ + D+D+Y+IAL+FRLLRQ+ Y++SS         K     
Sbjct: 86  IAYHFEEEIGEVIEQVYSNYDDDEDLYDIALRFRLLRQQGYNVSSDVFDKFKDCKGDFKK 145

Query: 136 SFVKDKRGILSLYEASHLGMKGE 147
             V D +G+LSL+EAS++ ++GE
Sbjct: 146 HLVNDVQGLLSLHEASYMSVQGE 161

[Close]

BLAST of CmaCh06G007130 vs. Swiss-Prot
Match: TPS2_RICCO (Probable terpene synthase 2 OS=Ricinus communis GN=TPS2 PE=3 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.0e-18
Identity = 58/144 (40.28%), Postives = 94/144 (65.28%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHH 75
           GD F+S  + + +  +E+ M E+Q+++LKE+V  M  +AD+ S+ L LIDL+QRLG+S+H
Sbjct: 28  GDRFLSCAAESTD--IEDDM-EQQVERLKEEVKKMIASADEPSQILNLIDLLQRLGVSYH 87

Query: 76  FELQINETLQL---LQSSSEVDDDIYNIALKFRLLRQEVYSIS----------SGKSLAP 135
           FE +I E LQ    + S S+ DDD++++AL+FRLLR++  ++S          +G  +  
Sbjct: 88  FEKEIEEALQQVLNMNSDSDKDDDLHSVALRFRLLREQGLNVSCDVFNKFRDRNGHFI-- 147

Query: 136 PSFVKDKRGILSLYEASHLGMKGE 147
            +   D +G+LSLYEA+H  + GE
Sbjct: 148 QTLKTDLQGMLSLYEAAHFRVHGE 166

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BLAST of CmaCh06G007130 vs. Swiss-Prot
Match: STS2_THAGA (Sesquiterpene synthase 2 OS=Thapsia garganica GN=STS2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.8e-18
Identity = 63/164 (38.41%), Postives = 93/164 (56.71%), Query Frame = 1


Query: 2   GCPSSICKLST----KCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKC 61
           G PSS+ + S        GD FI   ++ ++    ++  E+ LQ LK++V  M    D C
Sbjct: 10  GPPSSVVRNSAGFHPSIWGDTFIPSGNSAVQKTDVDRKEEENLQLLKQEVKKMLTAGDTC 69

Query: 62  SEKLR-LIDLIQRLGLSHHFELQINETLQLLQSS------SEVDDDIYNIALKFRLLRQE 121
            + L  LID IQRLGLS+HFE +I+  LQ ++ S      ++  D+++++AL FRLLRQE
Sbjct: 70  QQDLICLIDDIQRLGLSYHFEAEIDTLLQHVKDSYLEYYGTKNHDNLHDVALSFRLLRQE 129

Query: 122 VYSISS--------GKSLAPPSFVKDKRGILSLYEASHLGMKGE 147
            ++ISS                 VKD RG+LSL+EA+HL + GE
Sbjct: 130 GHNISSDVFSKFQDSDGKFNEKLVKDVRGMLSLFEAAHLSVHGE 173

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BLAST of CmaCh06G007130 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-17
Identity = 57/142 (40.14%), Postives = 86/142 (60.56%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCS-EKLRLIDLIQRLGLSH 75
           GD+F+SY S        +  +++ +Q+LKE+V  M + AD  S +KL LID IQRLG+++
Sbjct: 33  GDHFLSYASEFTNT---DDHLKQHVQQLKEEVRKMLMAADDDSAQKLLLIDAIQRLGVAY 92

Query: 76  HFELQINETLQLLQSSSEV--DDDIYNIALKFRLLRQEVYSIS--------SGKSLAPPS 135
           HFE +I+E L+ +   S V  ++D+Y  +L+FRLLRQ+ Y +S          +     S
Sbjct: 93  HFESEIDEVLKHMFDGSVVSAEEDVYTASLRFRLLRQQGYHVSCDLFNNFKDNEGNFKES 152

Query: 136 FVKDKRGILSLYEASHLGMKGE 147
              D RG+LSLYEA+H  + GE
Sbjct: 153 LSSDVRGMLSLYEATHFRVHGE 171

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BLAST of CmaCh06G007130 vs. TrEMBL
Match: A0A0A0L782_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040850 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.1e-26
Identity = 75/156 (48.08%), Postives = 102/156 (65.38%), Query Frame = 1


Query: 2   GCPSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKL 61
           G P    K  +  LG YF+S+   T+E   E+KM+ K+LQ+LKE+V +MFVT +K S+KL
Sbjct: 27  GVPHRSEKFQSSSLGHYFLSHQPKTLE---EDKMIRKKLQELKEEVRSMFVTTNKFSKKL 86

Query: 62  RLIDLIQRLGLSHHFELQINETLQLLQSSSEVDD---DIYNIALKFRLLRQEVYSIS--- 121
            LID IQRLGLS+HF+ +INE L L+++   VDD   D+Y  AL+FRLLRQ+ + +S   
Sbjct: 87  SLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEI 146

Query: 122 -----SGKSLAPPSFVKDKRGILSLYEASHLGMKGE 147
                + +     S  KD+  +LSLYEASHL MKGE
Sbjct: 147 FNKFTNERGELKESIRKDENSLLSLYEASHLRMKGE 179

[Close]

BLAST of CmaCh06G007130 vs. TrEMBL
Match: A0A0A0LN25_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G427840 PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 2.0e-25
Identity = 75/143 (52.45%), Postives = 97/143 (67.83%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHH 75
           GDYF+S  SN + N LEE  V K+L++LKE+  +MFV A+K SEKL LID +Q LGLS+H
Sbjct: 36  GDYFLSNISN-LSNLLEEDSVLKKLEELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYH 95

Query: 76  FELQINETLQLLQSSSEVD----DDIYNIALKFRLLRQEVYSIS--------SGKSLAPP 135
           FE +IN+ L  +Q+S+ VD    +D+Y +ALKFRLLRQ  + +S        S       
Sbjct: 96  FEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEILKKFTSESGDFKE 155

Query: 136 SFVKDKRGILSLYEASHLGMKGE 147
           S  KD+RG+LSLYEASHL MKGE
Sbjct: 156 SITKDERGLLSLYEASHLRMKGE 177

[Close]

BLAST of CmaCh06G007130 vs. TrEMBL
Match: A0A0A0L1X6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-22
Identity = 76/147 (51.70%), Postives = 98/147 (66.67%), Query Frame = 1


Query: 16  GDYFISYDSNTM---ENQLEEKMVEKQLQKLKEDVMNMFV-TADKCSEKLRLIDLIQRLG 75
           GDYFIS  SN+M   +N+ EE MVEK+L+KLKE+V+ M V T +   EKL LID IQRLG
Sbjct: 28  GDYFISSHSNSMGITKNEEEENMVEKELEKLKEEVIRMLVATTENSLEKLSLIDSIQRLG 87

Query: 76  LSHHFELQINETLQLLQSSSEVDD---DIYNIALKFRLLRQ-------EVYS-ISSGKSL 135
           LSH+F+ +INE L  +Q+S  +DD   DIY  ALKFRLLRQ       E+++  ++ +  
Sbjct: 88  LSHYFDREINEILN-IQNSFNLDDDDGDIYYTALKFRLLRQQGCFVSCEIFNKFTNEEGD 147

Query: 136 APPSFVKDKRGILSLYEASHLGMKGEL 148
              S VK KR I+SLYEAS   M GE+
Sbjct: 148 FKESAVKGKREIMSLYEASQWRMNGEI 173

[Close]

BLAST of CmaCh06G007130 vs. TrEMBL
Match: A0A0A0L2M0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040870 PE=3 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 5.6e-20
Identity = 62/123 (50.41%), Postives = 84/123 (68.29%), Query Frame = 1


Query: 35  MVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHHFELQINETLQLLQSSSEVD 94
           MV KQ++++ E V +MF  A KCSEKL LIDLIQRLGL ++FE +INE L L+ ++S +D
Sbjct: 1   MVMKQVEEMTEAVRSMFGDAKKCSEKLSLIDLIQRLGLYYYFEDEINEVLGLMHNASNLD 60

Query: 95  D---DIYNIALKFRLLRQEVYSIS--------SGKSLAPPSFVKDKRGILSLYEASHLGM 147
           +   D+Y +AL+FRLLRQ+ + +S        +       S  KD+ G+LSLYEASHL M
Sbjct: 61  EEDVDLYTMALRFRLLRQKGFFVSCEIFNKYTNESGDFKESITKDEVGLLSLYEASHLRM 120

[Close]

BLAST of CmaCh06G007130 vs. TrEMBL
Match: L0I517_MALDO (Germacrene-D synthase OS=Malus domestica GN=GDS PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 9.6e-20
Identity = 65/152 (42.76%), Postives = 92/152 (60.53%), Query Frame = 1


Query: 4   PSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTA-DKCSEKLR 63
           P      S    GD+F+SY S  ++ +LE+      +Q+LKE+V +M +T+ +  S+KL 
Sbjct: 20  PKPSATFSPSIWGDHFLSYASLEVDAELEQ-----HVQELKEEVRSMLMTSPENVSQKLN 79

Query: 64  LIDLIQRLGLSHHFELQINETLQLLQSSSEVDDDIYNIALKFRLLRQEVYSIS------- 123
           LID IQRLG+S+HF  +I E LQ +  SS   DD+Y +AL+FRLLRQ+ Y++S       
Sbjct: 80  LIDDIQRLGVSYHFGNEIEEILQKIHQSSYDLDDLYTVALRFRLLRQQGYNVSCDLFNKL 139

Query: 124 -SGKSLAPPSFVKDKRGILSLYEASHLGMKGE 147
             G      S V D  G+LSLYEA+HL + GE
Sbjct: 140 KDGDGKFKESLVDDVVGLLSLYEATHLRIHGE 166

[Close]

BLAST of CmaCh06G007130 vs. TAIR10
Match: AT4G16740.1 (AT4G16740.1 terpene synthase 03)

HSP 1 Score: 66.2 bits (160), Expect = 1.9e-11
Identity = 48/127 (37.80%), Postives = 73/127 (57.48%), Query Frame = 1


Query: 31  LEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHHFELQINETL------ 90
           ++E  VE+ +  LK++V  M    +   E+L LID +QRLG+S+HFE +I +TL      
Sbjct: 49  VKEDNVER-VTLLKQEVSKMLNETEGLLEQLELIDTLQRLGVSYHFEQEIKKTLTNVHVK 108

Query: 91  QLLQSSSEVD----DDIYNIALKFRLLRQEVYSISS---GKSLAPPSFVKDKRGILSLYE 145
            +    + +D     D+Y  AL+FRLLRQ  +SI+      ++      KD +GILSLYE
Sbjct: 109 NVRAHKNRIDRNRWGDLYATALEFRLLRQHGFSIAQDVFDGNIGVDLDDKDIKGILSLYE 168

[Close]

BLAST of CmaCh06G007130 vs. TAIR10
Match: AT3G25820.1 (AT3G25820.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 60.8 bits (146), Expect = 8.0e-10
Identity = 42/119 (35.29%), Postives = 67/119 (56.30%), Query Frame = 1


Query: 43  LKEDVMNMFVTADKCS-EKLRLIDLIQRLGLSHHFELQINETLQLLQS-------SSEVD 102
           LK+ V +M     K   E+L LID +Q+LG+S+HFE++IN+TL  L           + +
Sbjct: 81  LKKKVKSMLDDEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKE 140

Query: 103 DDIYNIALKFRLLRQEVYSISSG------KSLAPPSF-VKDKRGILSLYEASHLGMKGE 147
           +D++  +L+FRLLRQ  + +S          +   +F   +  GI+SLYEAS+L  K +
Sbjct: 141 EDLHATSLEFRLLRQHGFDVSENIFDVIIDQIESNTFKTNNINGIISLYEASYLSTKSD 199

[Close]

BLAST of CmaCh06G007130 vs. TAIR10
Match: AT3G25830.1 (AT3G25830.1 terpene synthase-like sequence-1,8-cineole)

HSP 1 Score: 60.8 bits (146), Expect = 8.0e-10
Identity = 42/119 (35.29%), Postives = 67/119 (56.30%), Query Frame = 1


Query: 43  LKEDVMNMFVTADKCS-EKLRLIDLIQRLGLSHHFELQINETLQLLQS-------SSEVD 102
           LK+ V +M     K   E+L LID +Q+LG+S+HFE++IN+TL  L           + +
Sbjct: 81  LKKKVKSMLDDEKKSRLEQLELIDDLQKLGVSYHFEIEINDTLTDLHLKMGRNCWKCDKE 140

Query: 103 DDIYNIALKFRLLRQEVYSISSG------KSLAPPSF-VKDKRGILSLYEASHLGMKGE 147
           +D++  +L+FRLLRQ  + +S          +   +F   +  GI+SLYEAS+L  K +
Sbjct: 141 EDLHATSLEFRLLRQHGFDVSENIFDVIIDQIESNTFKTNNINGIISLYEASYLSTKSD 199

[Close]

BLAST of CmaCh06G007130 vs. TAIR10
Match: AT3G29110.1 (AT3G29110.1 Terpenoid cyclases/Protein prenyltransferases superfamily protein)

HSP 1 Score: 60.1 bits (144), Expect = 1.4e-09
Identity = 47/155 (30.32%), Postives = 82/155 (52.90%), Query Frame = 1


Query: 3   CPSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTA---DKCSE 62
           C   I KL      +YF S     + +  E  ++E++++ LK +V  M +++   D   +
Sbjct: 27  CDRPISKLPPSKWTNYFHS----VLVDVSEMDVLEREIEALKPNVREMLMSSKGYDSVKK 86

Query: 63  KLRLIDLIQRLGLSHHFELQINETLQL-LQSSSEV---DDDIYNIALKFRLLRQEVYSIS 122
           +  +I L+  LGL++HFE +I ++L+   +   E+   +DD+Y I+  F + R   Y++S
Sbjct: 87  RSLMIYLLVSLGLAYHFEEEIEKSLKDGFEKIDEIIAGEDDLYTISTIFWVFRTYGYNMS 146

Query: 123 SG--------KSLAPPSFVKDKRGILSLYEASHLG 143
           S               S ++D RG+LSLYEA+HLG
Sbjct: 147 SDVFRRFKEENGKFKESLIEDARGMLSLYEAAHLG 177

[Close]

BLAST of CmaCh06G007130 vs. TAIR10
Match: AT1G61680.1 (AT1G61680.1 terpene synthase 14)

HSP 1 Score: 58.5 bits (140), Expect = 4.0e-09
Identity = 44/137 (32.12%), Postives = 71/137 (51.82%), Query Frame = 1


Query: 20  ISYDSNTMENQLE-EKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHHFEL 79
           +S  S    N+   E + E  ++K+K  +       D  SE L +ID+IQ LG+  HF  
Sbjct: 55  VSNPSKQFHNKTSLEYLHELNIKKIKNILS---ANVDVPSENLEMIDVIQSLGIDLHFRQ 114

Query: 80  QINETLQLL-QSSSEVDDDIYNIALKFRLLRQEVY--------SISSGKSLAPPSFVKDK 139
           +I +TL ++ +   + + D++ IAL+FRLLRQE +        +I   K         D 
Sbjct: 115 EIEQTLHMIYKEGLQFNGDLHEIALRFRLLRQEGHYVQEIIFKNILDKKGGFKDVVKNDV 174

Query: 140 RGILSLYEASHLGMKGE 147
           +G+  L+EAS L ++GE
Sbjct: 175 KGLTELFEASELRVEGE 188

[Close]

BLAST of CmaCh06G007130 vs. NCBI nr
Match: gi|778675681|ref|XP_011650452.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-26
Identity = 75/156 (48.08%), Postives = 102/156 (65.38%), Query Frame = 1


Query: 2   GCPSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKL 61
           G P    K  +  LG YF+S+   T+E   E+KM+ K+LQ+LKE+V +MFVT +K S+KL
Sbjct: 27  GVPHRSEKFQSSSLGHYFLSHQPKTLE---EDKMIRKKLQELKEEVRSMFVTTNKFSKKL 86

Query: 62  RLIDLIQRLGLSHHFELQINETLQLLQSSSEVDD---DIYNIALKFRLLRQEVYSIS--- 121
            LID IQRLGLS+HF+ +INE L L+++   VDD   D+Y  AL+FRLLRQ+ + +S   
Sbjct: 87  SLIDSIQRLGLSYHFDDEINEVLMLMKNPCNVDDEHEDLYITALRFRLLRQQGFFVSCEI 146

Query: 122 -----SGKSLAPPSFVKDKRGILSLYEASHLGMKGE 147
                + +     S  KD+  +LSLYEASHL MKGE
Sbjct: 147 FNKFTNERGELKESIRKDENSLLSLYEASHLRMKGE 179

[Close]

BLAST of CmaCh06G007130 vs. NCBI nr
Match: gi|659096216|ref|XP_008448975.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 126.7 bits (317), Expect = 3.4e-26
Identity = 78/157 (49.68%), Postives = 100/157 (63.69%), Query Frame = 1


Query: 2   GCPSSICKLSTKCLGDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKL 61
           G P    K     LG YF+S+   T++   E+KM++KQLQ+LKE+V +MFVT +K SEKL
Sbjct: 27  GVPRGSEKYQPSSLGHYFLSHQPKTLD---EDKMIKKQLQELKEEVRSMFVTIEKFSEKL 86

Query: 62  RLIDLIQRLGLSHHFELQINETLQLLQSS----SEVDDDIYNIALKFRLLRQEVYSIS-- 121
            LID IQRLGLS+HF+ +INE L LL  +     E  +D+Y  AL+FRLLRQ+   IS  
Sbjct: 87  SLIDSIQRLGLSYHFDDEINEVLMLLMQNPCNIDEEHEDLYITALRFRLLRQQGLFISCE 146

Query: 122 ------SGKSLAPPSFVKDKRGILSLYEASHLGMKGE 147
                 + +     S  KDK G+LSLYEASHL MKGE
Sbjct: 147 IFNKFTNERGEFKESIGKDKIGLLSLYEASHLRMKGE 180

[Close]

BLAST of CmaCh06G007130 vs. NCBI nr
Match: gi|778673649|ref|XP_004151916.2| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 123.6 bits (309), Expect = 2.9e-25
Identity = 75/143 (52.45%), Postives = 97/143 (67.83%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHH 75
           GDYF+S  SN + N LEE  V K+L++LKE+  +MFV A+K SEKL LID +Q LGLS+H
Sbjct: 36  GDYFLSNISN-LSNLLEEDSVLKKLEELKEEARSMFVAAEKHSEKLSLIDSLQCLGLSYH 95

Query: 76  FELQINETLQLLQSSSEVD----DDIYNIALKFRLLRQEVYSIS--------SGKSLAPP 135
           FE +IN+ L  +Q+S+ VD    +D+Y +ALKFRLLRQ  + +S        S       
Sbjct: 96  FEDEINKILDQIQNSAHVDEEDVEDLYIVALKFRLLRQRGFFVSCEILKKFTSESGDFKE 155

Query: 136 SFVKDKRGILSLYEASHLGMKGE 147
           S  KD+RG+LSLYEASHL MKGE
Sbjct: 156 SITKDERGLLSLYEASHLRMKGE 177

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BLAST of CmaCh06G007130 vs. NCBI nr
Match: gi|659125754|ref|XP_008462843.1| (PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-23
Identity = 73/137 (53.28%), Postives = 96/137 (70.07%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHH 75
           GDYF+S     + N LEE  V K+L++LKE   +MFV A+K S+KL LID IQ LGLS+H
Sbjct: 62  GDYFLS----NLSNILEEDSVLKKLEELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYH 121

Query: 76  FELQINETLQLLQSSSEVD----DDIYNIALKFRLLR-QEVYS-ISSGKSLAPPSFVKDK 135
           FE +INE L  +Q+S+ VD    +D+Y IALKFRLLR QE+++  ++       S  KD+
Sbjct: 122 FEDEINEILDQIQNSAHVDEEDEEDLYIIALKFRLLRQQEIFNKFTTESGDFKESITKDE 181

Query: 136 RGILSLYEASHLGMKGE 147
           +G+LSLYEASHL MKGE
Sbjct: 182 KGLLSLYEASHLRMKGE 194

[Close]

BLAST of CmaCh06G007130 vs. NCBI nr
Match: gi|659125752|ref|XP_008462842.1| (PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo])

HSP 1 Score: 118.2 bits (295), Expect = 1.2e-23
Identity = 73/143 (51.05%), Postives = 95/143 (66.43%), Query Frame = 1


Query: 16  GDYFISYDSNTMENQLEEKMVEKQLQKLKEDVMNMFVTADKCSEKLRLIDLIQRLGLSHH 75
           GDYF+S     + N LEE  V K+L++LKE   +MFV A+K S+KL LID IQ LGLS+H
Sbjct: 62  GDYFLS----NLSNILEEDSVLKKLEELKEAARSMFVAAEKHSDKLCLIDSIQCLGLSYH 121

Query: 76  FELQINETLQLLQSSSEVD----DDIYNIALKFRLLRQEVYSIS--------SGKSLAPP 135
           FE +INE L  +Q+S+ VD    +D+Y IALKFRLLRQ+ + +S        +       
Sbjct: 122 FEDEINEILDQIQNSAHVDEEDEEDLYIIALKFRLLRQQGFPVSCEIFNKFTTESGDFKE 181

Query: 136 SFVKDKRGILSLYEASHLGMKGE 147
           S  KD++G+LSLYEASHL MKGE
Sbjct: 182 SITKDEKGLLSLYEASHLRMKGE 200

The following BLAST results are available for this feature:

BLAST of CmaCh06G007130 vs. Swiss-Prot


Match Name	E-value	Identity	Description
AFSY1_CUCME	2.1e-20	45.52	Alpha-farnesene synthase OS=Cucumis melo PE=1 SV=1	[more]
TPS3_RICCO	3.1e-19	41.26	Probable terpene synthase 3 OS=Ricinus communis GN=TPS3 PE=3 SV=1	[more]
TPS2_RICCO	2.0e-18	40.28	Probable terpene synthase 2 OS=Ricinus communis GN=TPS2 PE=3 SV=1	[more]
STS2_THAGA	9.8e-18	38.41	Sesquiterpene synthase 2 OS=Thapsia garganica GN=STS2 PE=1 SV=1	[more]
TPSGD_VITVI	1.7e-17	40.14	(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1	[more]

BLAST of CmaCh06G007130 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L782_CUCSA	1.1e-26	48.08	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040850 PE=3 SV=1	[more]
A0A0A0LN25_CUCSA	2.0e-25	52.45	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G427840 PE=3 SV=1	[more]
A0A0A0L1X6_CUCSA	2.7e-22	51.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1	[more]
A0A0A0L2M0_CUCSA	5.6e-20	50.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040870 PE=3 SV=1	[more]
L0I517_MALDO	9.6e-20	42.76	Germacrene-D synthase OS=Malus domestica GN=GDS PE=2 SV=1	[more]

BLAST of CmaCh06G007130 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G16740.1	1.9e-11	37.80	terpene synthase 03	[more]
AT3G25820.1	8.0e-10	35.29	terpene synthase-like sequence-1,8-cineole	[more]
AT3G25830.1	8.0e-10	35.29	terpene synthase-like sequence-1,8-cineole	[more]
AT3G29110.1	1.4e-09	30.32	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT1G61680.1	4.0e-09	32.12	terpene synthase 14	[more]

BLAST of CmaCh06G007130 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778675681\|ref\|XP_011650452.1\|	1.5e-26	48.08	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|659096216\|ref\|XP_008448975.1\|	3.4e-26	49.68	PREDICTED: (-)-germacrene D synthase-like [Cucumis melo]	[more]
gi\|778673649\|ref\|XP_004151916.2\|	2.9e-25	52.45	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|659125754\|ref\|XP_008462843.1\|	1.2e-23	53.28	PREDICTED: probable terpene synthase 2 isoform X2 [Cucumis melo]	[more]
gi\|659125752\|ref\|XP_008462842.1\|	1.2e-23	51.05	PREDICTED: probable terpene synthase 2 isoform X1 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001906	Terpene_synth_N
IPR008930	Terpenoid_cyclase/PrenylTrfase

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

Vocabulary: **Molecular Function**
Term	Definition
GO:0010333	terpene synthase activity
GO:0016829	lyase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045338	farnesyl diphosphate metabolic process
biological_process	GO:0051762	sesquiterpene biosynthetic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0010334	sesquiterpene synthase activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0000287	magnesium ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G007130.1	CmaCh06G007130.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001906	Terpene synthase, N-terminal domain	GENE3D	G3DSA:1.50.30.10		coord: 36..146 score: 1.7
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 16..146 score: 4.0
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	unknown	SSF48239	Terpenoid cyclases/Protein prenyltransferases	coord: 14..147 score: 1.18
None	No IPR available	PANTHER	PTHR31225	FAMILY NOT NAMED	coord: 16..146 score: 3.4
None	No IPR available	PANTHER	PTHR31225:SF15	ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASE	coord: 16..146 score: 3.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh06G007130	Csa3G041380	Cucumber (Chinese Long) v2	cmacuB825
CmaCh06G007130	MELO3C014039	Melon (DHL92) v3.5.1	cmameB764
CmaCh06G007130	CSPI03G03590	Wild cucumber (PI 183967)	cmacpiB838
CmaCh06G007130	CsaV3_3G003660	Cucumber (Chinese Long) v3	cmacucB0975

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh06G007130	CmaCh16G010120	Cucurbita maxima (Rimu)	cmacmaB354

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh06G007130	Cucumber (Chinese Long) v3	cmacucB0990
CmaCh06G007130	Watermelon (97103) v2	cmawmbB836
CmaCh06G007130	Watermelon (97103) v2	cmawmbB845
CmaCh06G007130	Wax gourd	cmawgoB0988
CmaCh06G007130	Cucurbita maxima (Rimu)	cmacmaB334
CmaCh06G007130	Cucumber (Gy14) v1	cgycmaB0033
CmaCh06G007130	Cucumber (Gy14) v1	cgycmaB0839
CmaCh06G007130	Cucurbita moschata (Rifu)	cmacmoB803
CmaCh06G007130	Cucurbita moschata (Rifu)	cmacmoB804
CmaCh06G007130	Cucurbita moschata (Rifu)	cmacmoB829
CmaCh06G007130	Wild cucumber (PI 183967)	cmacpiB853
CmaCh06G007130	Cucumber (Chinese Long) v2	cmacuB838
CmaCh06G007130	Melon (DHL92) v3.5.1	cmameB746
CmaCh06G007130	Watermelon (Charleston Gray)	cmawcgB727
CmaCh06G007130	Watermelon (Charleston Gray)	cmawcgB733
CmaCh06G007130	Watermelon (97103) v1	cmawmB783
CmaCh06G007130	Watermelon (97103) v1	cmawmB805
CmaCh06G007130	Cucurbita pepo (Zucchini)	cmacpeB813
CmaCh06G007130	Cucurbita pepo (Zucchini)	cmacpeB814
CmaCh06G007130	Cucurbita pepo (Zucchini)	cmacpeB852
CmaCh06G007130	Bottle gourd (USVL1VR-Ls)	cmalsiB775
CmaCh06G007130	Cucumber (Gy14) v2	cgybcmaB438
CmaCh06G007130	Silver-seed gourd	carcmaB1285
CmaCh06G007130	Cucumber (Gy14) v2	cgybcmaB695
CmaCh06G007130	Melon (DHL92) v3.6.1	cmamedB843
CmaCh06G007130	Melon (DHL92) v3.6.1	cmamedB869
CmaCh06G007130	Silver-seed gourd	carcmaB0025
CmaCh06G007130	Silver-seed gourd	carcmaB1203