CmaCh04G022590 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh04G022590 vs. Swiss-Prot
Match: AAE5_ARATH (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana GN=AAE5 PE=2 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 8.1e-211
Identity = 354/549 (64.48%), Postives = 438/549 (79.78%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+Y D  SI+YG NT YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
              +L   A+ +   +   P+LV +AD    E   + V     F  +Y+ +I +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIADKEE-EGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           ANGWS+ WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAGAP PA +L R ES+GF ++HGYGLTET G  V C+WK +W++LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ ++
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480
           GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY NP+VNE AVVARP  
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLTQ+PTE E+IEYCR K+P++M PKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LKEMAMSMG 549
           L+E+A  MG
Sbjct: 541 LREIAKKMG 545

BLAST of CmaCh04G022590 vs. Swiss-Prot
Match: AAE6_ARATH (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana GN=AAE6 PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 3.8e-208
Identity = 349/549 (63.57%), Postives = 438/549 (79.78%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+Y D  SI+YG NT YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
              +L   A+ +   +   P+LV++AD    E   + V     F  +Y+ +I +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADKEE-EGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           +NGW+  WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAG+P PAT+L R ES+GF ++HGYGLTET G +V C+WK +W+ LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ ++
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480
           GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY  P+VNE AVVARP E
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + +PTEEE++EYCR K+P++M PKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LKEMAMSMG 549
           L+++A  MG
Sbjct: 541 LRDIAKKMG 545

BLAST of CmaCh04G022590 vs. Swiss-Prot
Match: AAE8_ARATH (Probable acyl-activating enzyme 8 OS=Arabidopsis thaliana GN=AAE8 PE=2 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.3e-204
Identity = 353/549 (64.30%), Postives = 433/549 (78.87%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTT-YTWSDTHRRCLQLASSLSSI 60
           ME LKP  ANS PLT +GFLERAAT+Y D  SI+YGN+T YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
            + +L   A+ +    +N P+LVL+A++   EE  + V     F   Y  +I +G+ +F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEE-EEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG F++TLD L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDI-RPL 300
           ANGW++ WG+AAVGGTN+C+RK  A  +Y LIR H VTH+ GAP+VL +L+ S +  +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PV  LTAG+  PAT+L R ESLGF V+HGYGLTET G +V C+WK  W++LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LKSRQGVRTV    +DVVD E+G SV RDG ++GEIVLR  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           +GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLY NP+VNEAAVVARP 
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT++PT++EIIEYC+ K+PR+MAPKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLKEMAMSM 548
           LLKE+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmaCh04G022590 vs. Swiss-Prot
Match: AEE9_ARATH (Probable acyl-activating enzyme 9 OS=Arabidopsis thaliana GN=AEE9 PE=2 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 6.5e-200
Identity = 341/555 (61.44%), Postives = 427/555 (76.94%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GFL+RAA++Y D PSI++  NT +TWS+TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           S+GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DRAFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSE 180
           D    +++  A+  F  +  +P LVL+ DD   E+ S S     +F+D+Y+G++ +GDS 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P++LNYTSGTTSSPKGVV  HR  F++T+  LL+W  P + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRP 300
           FHANGW ++WG AAVG TN+C R+ DA  +Y LI +HHVTH+C AP+VLNMLTN P  +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERA 360
           L  PV+++TAGAP PA I+ R E+LGF+V HGYGLTETGG VV C+WKAEWD L  +ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMT 420
           RLKSRQGVRT+    VDV D  TG+SV  DGVS+GEIVL+ GS+MLGY+KD E TA  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 EDGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARP 480
           EDGWF +GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLY NP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 HEFWGETPCAFVSLR-----EGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTST 540
            + WGETPCAFVSL+      GL    TE EI E+C+ +LP++M P+ V+F  ELPKTST
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKIQKYLLKEMAMSM 548
           GKIQK+LL++MA S+
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CmaCh04G022590 vs. Swiss-Prot
Match: AAE4_ARATH (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana GN=AEE4 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.5e-196
Identity = 328/549 (59.74%), Postives = 421/549 (76.68%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSS-- 60
           ME L P  +NS PLT +GFLERAA+++ DSPS+++  T +TWS+TH RCL++AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD 120
           +GI RG VVSVI PN P +YEL FAVPM+GA+LN IN RLDA  +S+LL HSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 RAFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEF 180
               +L+  A+   P +  RP LV++ D      SSS+ +   +F+D+YEG + +GD  F
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM---DFLDTYEGFMERGDLRF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEW P+VLNYTSGTTSSPKGVVH HR  F+ T++ LL+W +P + VYLWTLPMF
Sbjct: 181 KWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           HANGWS++W  AAVG  NIC+ + D   +++LI ++ VTH+C AP+VLNMLTN P  +PL
Sbjct: 241 HANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PVK++TAGAP PAT++ + E+LGFDV+HGYG+TETGG VV C+ K EWD+L   ERA+
Sbjct: 301 QSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAK 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
            KSRQG+RT     VDV D  +G+SV  DG ++GEIV R GS+MLGY+KD E TA SM E
Sbjct: 361 QKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMRE 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           DGWF TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLY NP++ EAAVVA+P 
Sbjct: 421 DGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           + WGETPCAFVSL+       TE EI E+C+ KLP++M P+ VVF+ ELPKTSTGKIQK+
Sbjct: 481 KMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKF 540

Query: 541 LLKEMAMSM 548
           LL++MA S+
Sbjct: 541 LLRQMAKSL 544

BLAST of CmaCh04G022590 vs. TrEMBL
Match: A0A0A0KMT3_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G318390 PE=4 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 1.2e-258
Identity = 429/552 (77.72%), Postives = 487/552 (88.22%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LKPRPANS PLTPIGF+ERAA IY DS SIIY +TTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           IQRGHVVSV +PNTP MYELHFAVPM GAILNTIN RLD RTISILL HSESKLIFVD+ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESS--SSVLIEGNFVDSYEGMIRKGDSEF 180
            C LI +AL LFPP++ RP+LVL+ DD A ++SS  S V I  NFVDSYE M++ GDS+F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG F V LD +LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           H NGWSF WG+AAVGGTN+CIRKFD+ L++SLIRRHHVTHLCGAPVVLNMLTNSPD  PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
           D PVKILTAGAP PA++L R ESLGF VTHGYGLTE+ GT++CCSWK+EW+QLPA+E+AR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LK+RQGVR +A  AVDV+D ET +SV RDG S+GEIV+R  SLMLGY KD EAT+K+M+E
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           DGWF TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARP 
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAF+SL+EGL+++PTEEEIIEYC+GKLP+FM PKTV+FMTE+PKT+TGK QK+
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 LLKEMAMSMGPP 551
           +L++ A S+GPP
Sbjct: 541 VLRQKAKSLGPP 552

BLAST of CmaCh04G022590 vs. TrEMBL
Match: A0A0A0KT33_CUCSA (AMP dependent ligase OS=Cucumis sativus GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 895.6 bits (2313), Expect = 3.1e-257
Identity = 434/548 (79.20%), Postives = 483/548 (88.14%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           MEHLKP PANSSPLTPIGFLERA+ IYADSPSIIY +TT+TWS T+RRCL+LASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I +GHVVSVI+PNTPPMYEL FAVPM GAIL+ IN RLDARTIS+ L HSESKL+FVD+A
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180
              LI +AL LFPPE  RP+LVL++DDAA E+S+SSV I G+ VD YE M++KGD EF+W
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRP SEWDPIV+NYTSGTTSSPKGVVH HRG F VTLD L+EWGVPK SVYLWTLPMFHA
Sbjct: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261

Query: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSF + +AAVGGTNICIRKFDA L++SLI RHHVTH+CGAPVVLNMLTNSP+ RPL+ 
Sbjct: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321

Query: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAP PA +L R ESLGFDV+HGYGLTETGG V+ CSWK EW+QLPA ERARLK
Sbjct: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420
           +RQGVRT+A    DV+D  TG+ V RDGVS+GEIV+R GS+MLGYFKD EAT K+MTE+G
Sbjct: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441

Query: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP+VNEAAVVARP EF
Sbjct: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501

Query: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+ PTEEEIIEYC+GKL  FM PKTVVFM +LPKTSTGKIQK+LL
Sbjct: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561

Query: 541 KEMAMSMG 549
           +EMA SMG
Sbjct: 562 REMAKSMG 569

BLAST of CmaCh04G022590 vs. TrEMBL
Match: A0A061ESR0_THECC (Acyl activating enzyme 5 OS=Theobroma cacao GN=TCM_020359 PE=4 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 1.7e-236
Identity = 391/550 (71.09%), Postives = 464/550 (84.36%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LKPRPA+SSPLTP+GFLERAAT+Y D  SIIY NT+YTWSDTHRRCLQ+ASSLSS G
Sbjct: 51  MEELKPRPASSSPLTPLGFLERAATVYGDCTSIIYNNTSYTWSDTHRRCLQIASSLSSTG 110

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I+RGHVVSV++PN P MYELHFAVPMTGA+LN+INTRLDAR +SILL H ESKL+FVD  
Sbjct: 111 IKRGHVVSVLAPNIPAMYELHFAVPMTGAVLNSINTRLDARIVSILLRHCESKLLFVDTL 170

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESS--SSVLIEGNFVDSYEGMIRKGDSEF 180
             +L   A+ LFPP    P+LVL+ DD+A  ESS  SS  ++  F  +YE +++KGD +F
Sbjct: 171 SRSLALEAISLFPPNTKPPLLVLIGDDSADAESSDSSSSTVDSRFCCTYESLVKKGDPDF 230

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEW+PIVLNYTSGTTSSPKGVVH HRG+F++TLD L++WGVPKQ VYLWTLPMF
Sbjct: 231 KWVRPQSEWNPIVLNYTSGTTSSPKGVVHSHRGTFMMTLDSLIDWGVPKQPVYLWTLPMF 290

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           HANGWSF+WG+AAVGG N+C+RKFDA ++YSLI++H VTH+CGAPVVL+ML+N P+I+PL
Sbjct: 291 HANGWSFTWGIAAVGGINVCVRKFDAPIIYSLIKKHGVTHMCGAPVVLSMLSNFPEIKPL 350

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PVKILTAGAP PA +LFR E+LGF V+HGYGLTETGG VV C+WK  W++LPA ERAR
Sbjct: 351 QSPVKILTAGAPPPAAVLFRTEALGFIVSHGYGLTETGGLVVSCAWKRGWNKLPATERAR 410

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LK+RQGVRT+     DVVD E+G SV RDG ++GEIVLR   +MLGY KD   T K M E
Sbjct: 411 LKARQGVRTLGMTEADVVDTESGLSVKRDGSTLGEIVLRGACIMLGYLKDPNGTNKCMKE 470

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           +GWF TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P++ EAAVVARP 
Sbjct: 471 NGWFFTGDVGVMHPDGYMEIKDRSKDVIISGGENLSSVEVESILYNHPAIIEAAVVARPD 530

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           ++WGETPCAFVSL+ GLTQ+P+E+EIIEYCR KLP +M PKTVVF  ELPKTSTGKIQK+
Sbjct: 531 DYWGETPCAFVSLKAGLTQKPSEKEIIEYCRAKLPHYMVPKTVVFKEELPKTSTGKIQKF 590

Query: 541 LLKEMAMSMG 549
           LL+E+A  MG
Sbjct: 591 LLREIAKGMG 600

BLAST of CmaCh04G022590 vs. TrEMBL
Match: A0A0D2VMX2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G160500 PE=4 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 2.2e-231
Identity = 386/548 (70.44%), Postives = 454/548 (82.85%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LK RPANSSPLTP+GFL+RAAT+Y D  SII+ NT+YTWS+THRRCLQLASSLSS G
Sbjct: 1   MEELKSRPANSSPLTPLGFLDRAATVYGDCTSIIHNNTSYTWSETHRRCLQLASSLSSTG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I+ GHVVSV++PN P MYELHFAVPM+GA+LN+INTRLDART+SILLCHSESKL+FVD  
Sbjct: 61  IKTGHVVSVLAPNIPAMYELHFAVPMSGAVLNSINTRLDARTVSILLCHSESKLLFVDTL 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180
             +L   A+ LFPP  N P+LVL+ DD   E+   S  I+  F  +YE ++ KG+  F+W
Sbjct: 121 SQSLALEAISLFPPNENPPLLVLIKDD---EDDGVSSTIDSRFCCTYESLVEKGNPGFKW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRP SEW+PIVLNYTSGTTSSPKGVVHCHR  F  T+D L+ W VPKQ VYLWTLPMFHA
Sbjct: 181 IRPESEWNPIVLNYTSGTTSSPKGVVHCHRAVFTNTVDSLIHWEVPKQPVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSF+WGMAAVGGTNIC+RKFDA ++YSLIR+H VTH+CGAPVVLNML+NSP+ +PL  
Sbjct: 241 NGWSFTWGMAAVGGTNICVRKFDARIIYSLIRKHGVTHMCGAPVVLNMLSNSPETKPLQN 300

Query: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PV+ILTAGAP PA +L R ESLGF V+HGYGLTETGG VV C+WK EW++ P  ERARLK
Sbjct: 301 PVQILTAGAPPPAAVLSRTESLGFIVSHGYGLTETGGLVVSCAWKREWNKFPLAERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420
           +RQGVRT+     D+VD E+G SV RDG S+GEIVLR   +MLGY KD  AT+K + EDG
Sbjct: 361 ARQGVRTLTVTEADIVDPESGLSVKRDGSSLGEIVLRGPCIMLGYLKDPNATSKCLKEDG 420

Query: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480
           WF TGDVGV+HPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P++NEAAVVARP E+
Sbjct: 421 WFYTGDVGVIHPDGYMEIKDRSKDVIISGGENLSSVEVESILYTHPAINEAAVVARPDEY 480

Query: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSL+  LT++P+E EI+EYCR KLP +M PKTVVF  ELPKTSTGKI K++L
Sbjct: 481 WGETPCAFVSLKAELTRKPSEIEIMEYCRAKLPHYMVPKTVVFKDELPKTSTGKILKFVL 540

Query: 541 KEMAMSMG 549
           +E+A  MG
Sbjct: 541 REIAKGMG 545

BLAST of CmaCh04G022590 vs. TrEMBL
Match: A0A067FAF3_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g045072mg PE=4 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 2.9e-231
Identity = 384/549 (69.95%), Postives = 451/549 (82.15%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           M+ LKP  ANSSPLTP+GFLERAAT+Y D PSIIY N  YTWS THRRCLQLASSLSSIG
Sbjct: 1   MDELKPCTANSSPLTPLGFLERAATVYGDCPSIIYNNLIYTWSQTHRRCLQLASSLSSIG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I RGHVVSV++PN P MYE HFA+P TGAILN INTRLDARTIS+LL HSESKL+ VD  
Sbjct: 61  ITRGHVVSVVAPNIPAMYEAHFAIPFTGAILNNINTRLDARTISVLLQHSESKLVLVDYQ 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAE-ESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
             NL+  A+ LFP +I  P LVL+AD    + ++ SS  ++ NF  +YE ++ KGD  F+
Sbjct: 121 SRNLVVEAISLFPSDIKCPPLVLIADHTDHDNDNKSSQTVDSNFCCTYESLVTKGDPNFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP +EWDP++LNYTSGTTSSPKGVVHCHRG F++T+D L++W +PKQ VYLWTLPMFH
Sbjct: 181 WIRPQNEWDPMILNYTSGTTSSPKGVVHCHRGIFVMTVDSLIDWSIPKQPVYLWTLPMFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           ANGWS+ WGMAAVGGTN+C+RKFDA ++Y +IR+H VTH+CGAPVVLNM++N P   PL 
Sbjct: 241 ANGWSYPWGMAAVGGTNVCLRKFDAAVIYKMIRQHGVTHMCGAPVVLNMISNLPRSEPLR 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAGAP PA +LFR ESLGF V+HGYGLTET G VV C+WK +W++LPA ERAR+
Sbjct: 301 NPVHILTAGAPPPAAVLFRTESLGFLVSHGYGLTETAGLVVSCAWKNKWNRLPATERARM 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420
           KSRQGVRTV    +DV+D E+G SV+ DG S+GEIV R GS+MLGY KD   T K M +D
Sbjct: 361 KSRQGVRTVCFTEIDVIDSESGLSVSHDGASLGEIVFRGGSMMLGYLKDPNGTRKCMKDD 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480
           GWF TGDVGV+H DGYLEIKDRSKDVIISGGENLSSVEVESVLY NP+VNEAAVVARP E
Sbjct: 421 GWFYTGDVGVIHADGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAF+SL+  L ++PTE+EIIEYCR +LPR+M PKTVVF  ELPKTSTGKI+K+ 
Sbjct: 481 FWGETPCAFLSLKAELEKKPTEKEIIEYCRARLPRYMVPKTVVFKEELPKTSTGKIKKFE 540

Query: 541 LKEMAMSMG 549
           L+EMA +MG
Sbjct: 541 LREMAKAMG 549

BLAST of CmaCh04G022590 vs. TAIR10
Match: AT5G16370.1 (AT5G16370.1 acyl activating enzyme 5)

HSP 1 Score: 734.6 bits (1895), Expect = 4.6e-212
Identity = 354/549 (64.48%), Postives = 438/549 (79.78%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+Y D  SI+YG NT YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H  SKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
              +L   A+ +   +   P+LV +AD    E   + V     F  +Y+ +I +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTD--PPILVFIADKEE-EGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           ANGWS+ WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAGAP PA +L R ES+GF ++HGYGLTET G  V C+WK +W++LPA +RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ ++
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480
           GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY NP+VNE AVVARP  
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ GLTQ+PTE E+IEYCR K+P++M PKTV F+ ELPKTSTGK+ K++
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 541 LKEMAMSMG 549
           L+E+A  MG
Sbjct: 541 LREIAKKMG 545

BLAST of CmaCh04G022590 vs. TAIR10
Match: AT5G16340.1 (AT5G16340.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 725.7 bits (1872), Expect = 2.1e-209
Identity = 349/549 (63.57%), Postives = 438/549 (79.78%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSLSSI 60
           ME +KP  ANS PLTPIGFLERAAT+Y D  SI+YG NT YTW +T+ RCL++ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+SPNTP MYEL FAVPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
              +L   A+ +   +   P+LV++AD    E   + V     F  +Y+ +I +GD  F+
Sbjct: 121 FSVDLAVEAVSMMTTD--PPILVVIADKEE-EGGVADVADLSKFSYTYDDLIERGDPGFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDP+VLNYTSGTTS+PKGVVHCHRG F++++D L++W VPK  VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLD 300
           +NGW+  WG+AAVGGTN+C+RKFDA L+Y LIR H VTH+CGAPVVLNML+ + + +PL+
Sbjct: 241 SNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQESQPLN 300

Query: 301 WPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARL 360
            PV ILTAG+P PAT+L R ES+GF ++HGYGLTET G +V C+WK +W+ LPA +RARL
Sbjct: 301 HPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPASDRARL 360

Query: 361 KSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTED 420
           K+RQGVRTV    +DVVD E+G SV R+G ++GEIV+R  S+MLGY KD   T K++ ++
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKAL-KN 420

Query: 421 GWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHE 480
           GWF TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLY  P+VNE AVVARP E
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDE 480

Query: 481 FWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYL 540
           FWGETPCAFVSL+ G + +PTEEE++EYCR K+P++M PKTV FM ELPK+STGK+ K++
Sbjct: 481 FWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFV 540

Query: 541 LKEMAMSMG 549
           L+++A  MG
Sbjct: 541 LRDIAKKMG 545

BLAST of CmaCh04G022590 vs. TAIR10
Match: AT1G75960.1 (AT1G75960.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 712.6 bits (1838), Expect = 1.9e-205
Identity = 353/549 (64.30%), Postives = 433/549 (78.87%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTT-YTWSDTHRRCLQLASSLSSI 60
           ME LKP  ANS PLT +GFLERAAT+Y D  SI+YGN+T YTW +T+ RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 61  GIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDR 120
           GI R  VVSV+S NTP MYEL F+VPM+GAILN INTRLDART+S+LL H ESKL+FVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 121 AFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQ 180
            + +L   A+ +    +N P+LVL+A++   EE  + V     F   Y  +I +G+ +F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEE-EEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 181 WIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFH 240
           WIRP SEWDPIV+NYTSGTTSSPKGVVHCHRG F++TLD L +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 241 ANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDI-RPL 300
           ANGW++ WG+AAVGGTN+C+RK  A  +Y LIR H VTH+ GAP+VL +L+ S +  +PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PV  LTAG+  PAT+L R ESLGF V+HGYGLTET G +V C+WK  W++LPA ++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LKSRQGVRTV    +DVVD E+G SV RDG ++GEIVLR  S+MLGY K+   T  S  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           +GWF TGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLY NP+VNEAAVVARP 
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAFVSL+ GLT++PT++EIIEYC+ K+PR+MAPKTV F+ ELPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 541 LLKEMAMSM 548
           LLKE+A +M
Sbjct: 541 LLKEIAKNM 544

BLAST of CmaCh04G022590 vs. TAIR10
Match: AT1G21540.1 (AT1G21540.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 698.4 bits (1801), Expect = 3.6e-201
Identity = 341/555 (61.44%), Postives = 427/555 (76.94%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYG-NTTYTWSDTHRRCLQLASSL--S 60
           ME L P P+NS+PLT +GFL+RAA++Y D PSI++  NT +TWS+TH RCL++AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 61  SIGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFV 120
           S+GI RG VVSV+ PN P +YEL FAVPM+GAILN IN RLDA  +S+LL HSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 121 DRAFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSE 180
           D    +++  A+  F  +  +P LVL+ DD   E+ S S     +F+D+Y+G++ +GDS 
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 181 FQWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPM 240
           F+WIRP +EW P++LNYTSGTTSSPKGVV  HR  F++T+  LL+W  P + VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 241 FHANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRP 300
           FHANGW ++WG AAVG TN+C R+ DA  +Y LI +HHVTH+C AP+VLNMLTN P  +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 301 LDWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERA 360
           L  PV+++TAGAP PA I+ R E+LGF+V HGYGLTETGG VV C+WKAEWD L  +ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 361 RLKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMT 420
           RLKSRQGVRT+    VDV D  TG+SV  DGVS+GEIVL+ GS+MLGY+KD E TA  M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 421 EDGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARP 480
           EDGWF +GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLY NP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 481 HEFWGETPCAFVSLR-----EGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTST 540
            + WGETPCAFVSL+      GL    TE EI E+C+ +LP++M P+ V+F  ELPKTST
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLV---TEREIREFCKTRLPKYMVPRKVIFQEELPKTST 540

Query: 541 GKIQKYLLKEMAMSM 548
           GKIQK+LL++MA S+
Sbjct: 541 GKIQKFLLRQMAKSL 549

BLAST of CmaCh04G022590 vs. TAIR10
Match: AT1G77240.1 (AT1G77240.1 AMP-dependent synthetase and ligase family protein)

HSP 1 Score: 687.2 bits (1772), Expect = 8.4e-198
Identity = 328/549 (59.74%), Postives = 421/549 (76.68%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSS-- 60
           ME L P  +NS PLT +GFLERAA+++ DSPS+++  T +TWS+TH RCL++AS+LSS  
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 61  IGIQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVD 120
           +GI RG VVSVI PN P +YEL FAVPM+GA+LN IN RLDA  +S+LL HSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 121 RAFCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEF 180
               +L+  A+   P +  RP LV++ D      SSS+ +   +F+D+YEG + +GD  F
Sbjct: 121 HHSSSLVLEAVSFLPKD-ERPRLVILNDGNDMPSSSSADM---DFLDTYEGFMERGDLRF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEW P+VLNYTSGTTSSPKGVVH HR  F+ T++ LL+W +P + VYLWTLPMF
Sbjct: 181 KWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           HANGWS++W  AAVG  NIC+ + D   +++LI ++ VTH+C AP+VLNMLTN P  +PL
Sbjct: 241 HANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PVK++TAGAP PAT++ + E+LGFDV+HGYG+TETGG VV C+ K EWD+L   ERA+
Sbjct: 301 QSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAK 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
            KSRQG+RT     VDV D  +G+SV  DG ++GEIV R GS+MLGY+KD E TA SM E
Sbjct: 361 QKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMRE 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           DGWF TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLY NP++ EAAVVA+P 
Sbjct: 421 DGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           + WGETPCAFVSL+       TE EI E+C+ KLP++M P+ VVF+ ELPKTSTGKIQK+
Sbjct: 481 KMWGETPCAFVSLKYH-DGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKF 540

Query: 541 LLKEMAMSM 548
           LL++MA S+
Sbjct: 541 LLRQMAKSL 544

BLAST of CmaCh04G022590 vs. NCBI nr
Match: gi|659126420|ref|XP_008463174.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 910.2 bits (2351), Expect = 1.7e-261
Identity = 438/551 (79.49%), Postives = 489/551 (88.75%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LKP PANS PLTPIGF++RAATIY DS SIIY +TTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPTPANSFPLTPIGFIQRAATIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           IQRGHVVSVI+PNTP MYELHFAVPM GAILNTIN RLD RTISILL HSESKLIFVD+A
Sbjct: 61  IQRGHVVSVIAPNTPAMYELHFAVPMVGAILNTINLRLDTRTISILLRHSESKLIFVDQA 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESS--SSVLIEGNFVDSYEGMIRKGDSEF 180
            C LI  AL LFPPE+ RP+LVL+ADDAA ++SS  S V I  NFVDSYE M+++GDS+F
Sbjct: 121 SCALIFEALALFPPEMKRPLLVLIADDAAEQDSSLPSFVSIRDNFVDSYEDMVKRGDSKF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTSSPKGVVHCHRG F V LD LLEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVALDSLLEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           H NGWSF WG+AAVGGTN+CIRKFDA L++SLIRRHHVTHLCGAPVVLNMLTNSPD  PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDARLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
           D PVKILTAGAP PA+IL R ESLGF VTHGYGLTE+ GT++CCSWK+EW+QLPA+E+AR
Sbjct: 301 DRPVKILTAGAPPPASILLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LK+RQGVR +A  AVDV+D ET +SV RDG S+GEIV+R  S+ LGY KD E TAK+M+E
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGASVTLGYLKDPEGTAKAMSE 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           DGWF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARP 
Sbjct: 421 DGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAF+SL+EGL+++PTEEEIIEYC+GKLPRFM PKTV+FMTE+PKT+TGKIQK+
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPRFMVPKTVIFMTEMPKTATGKIQKF 540

Query: 541 LLKEMAMSMGP 550
           +L++ A S+GP
Sbjct: 541 VLRQKAKSLGP 551

BLAST of CmaCh04G022590 vs. NCBI nr
Match: gi|449462358|ref|XP_004148908.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 900.2 bits (2325), Expect = 1.8e-258
Identity = 429/552 (77.72%), Postives = 487/552 (88.22%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LKPRPANS PLTPIGF+ERAA IY DS SIIY +TTYTWS+THRRCLQLASS+SS G
Sbjct: 1   MEDLKPRPANSFPLTPIGFIERAAIIYGDSTSIIYNSTTYTWSETHRRCLQLASSISSFG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           IQRGHVVSV +PNTP MYELHFAVPM GAILNTIN RLD RTISILL HSESKLIFVD+ 
Sbjct: 61  IQRGHVVSVFAPNTPAMYELHFAVPMAGAILNTINLRLDTRTISILLRHSESKLIFVDQP 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESS--SSVLIEGNFVDSYEGMIRKGDSEF 180
            C LI +AL LFPP++ RP+LVL+ DD A ++SS  S V I  NFVDSYE M++ GDS+F
Sbjct: 121 SCALIFDALALFPPDMKRPLLVLITDDTAEQDSSLPSVVSIRDNFVDSYENMVKSGDSKF 180

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           QWIRP SEWDPIVLNYTSGTTS+PKGVV+CHRG F V LD +LEWGVPKQSVYLWTLPMF
Sbjct: 181 QWIRPLSEWDPIVLNYTSGTTSAPKGVVNCHRGVFTVALDSILEWGVPKQSVYLWTLPMF 240

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           H NGWSF WG+AAVGGTN+CIRKFD+ L++SLIRRHHVTHLCGAPVVLNMLTNSPD  PL
Sbjct: 241 HGNGWSFPWGIAAVGGTNVCIRKFDSRLIFSLIRRHHVTHLCGAPVVLNMLTNSPDNLPL 300

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
           D PVKILTAGAP PA++L R ESLGF VTHGYGLTE+ GT++CCSWK+EW+QLPA+E+AR
Sbjct: 301 DRPVKILTAGAPPPASVLLRTESLGFVVTHGYGLTESAGTIICCSWKSEWNQLPAIEQAR 360

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LK+RQGVR +A  AVDV+D ET +SV RDG S+GEIV+R  SLMLGY KD EAT+K+M+E
Sbjct: 361 LKARQGVRMLAVEAVDVLDPETRKSVKRDGASIGEIVVRGPSLMLGYLKDTEATSKAMSE 420

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           DGWF TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVESVLY NP VNEAAVVARP 
Sbjct: 421 DGWFFTGDVGVMHPDGYIEIKDRSKDVIISGGENLSSVEVESVLYMNPMVNEAAVVARPD 480

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           EFWGETPCAF+SL+EGL+++PTEEEIIEYC+GKLP+FM PKTV+FMTE+PKT+TGK QK+
Sbjct: 481 EFWGETPCAFISLKEGLSRKPTEEEIIEYCKGKLPKFMVPKTVIFMTEMPKTATGKFQKF 540

Query: 541 LLKEMAMSMGPP 551
           +L++ A S+GPP
Sbjct: 541 VLRQKAKSLGPP 552

BLAST of CmaCh04G022590 vs. NCBI nr
Match: gi|659126423|ref|XP_008463175.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo])

HSP 1 Score: 899.4 bits (2323), Expect = 3.0e-258
Identity = 436/549 (79.42%), Postives = 486/549 (88.52%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           M+HLKP PANSSPLTPI FLERA+ +YADSPSIIY +T++TWS T+ RCL+LASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I +GHVVSVISPNTPPMYEL F+VPM GAIL+ IN RLDARTIS+ L HSESKL+FVD+A
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180
              LI +AL LFPPE  RP+LVL+ADDAA E+S+SSV I G+FVD YE MI+KGD EF+W
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRP SEWDPIV+NYTSGTTSSPKGVVH HRG F VTLD L+EWGVPK SVYLWTLPMFHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSF + +AAVGGTNICIRKFDA L++SLI RHHVTHLCGAPVVLNMLTNSP+ RPL+ 
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAP PA +LFR ESLGFDV+HGYGLTETGG V+ CSWK +W+QLPA ERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420
           +RQGVRT+A    DV+D ETG+SV RDGVS+GEIVLR GS+MLGYFKD EAT K+MTE+G
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP+VNEAAVVARP EF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGL++ PTEEEIIEYCRGKL  FM PKTVVFM +LPKTSTGKIQK+LL
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 541 KEMAMSMGP 550
           +EMA SMGP
Sbjct: 541 REMAKSMGP 549

BLAST of CmaCh04G022590 vs. NCBI nr
Match: gi|449462360|ref|XP_004148909.1| (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus])

HSP 1 Score: 895.6 bits (2313), Expect = 4.4e-257
Identity = 434/548 (79.20%), Postives = 483/548 (88.14%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           MEHLKP PANSSPLTPIGFLERA+ IYADSPSIIY +TT+TWS T+RRCL+LASSLSSIG
Sbjct: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I +GHVVSVI+PNTPPMYEL FAVPM GAIL+ IN RLDARTIS+ L HSESKL+FVD+A
Sbjct: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESSSSVLIEGNFVDSYEGMIRKGDSEFQW 180
              LI +AL LFPPE  RP+LVL++DDAA E+S+SSV I G+ VD YE M++KGD EF+W
Sbjct: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201

Query: 181 IRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMFHA 240
           IRP SEWDPIV+NYTSGTTSSPKGVVH HRG F VTLD L+EWGVPK SVYLWTLPMFHA
Sbjct: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261

Query: 241 NGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPLDW 300
           NGWSF + +AAVGGTNICIRKFDA L++SLI RHHVTH+CGAPVVLNMLTNSP+ RPL+ 
Sbjct: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321

Query: 301 PVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERARLK 360
           PVKILTAGAP PA +L R ESLGFDV+HGYGLTETGG V+ CSWK EW+QLPA ERARLK
Sbjct: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381

Query: 361 SRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTEDG 420
           +RQGVRT+A    DV+D  TG+ V RDGVS+GEIV+R GS+MLGYFKD EAT K+MTE+G
Sbjct: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441

Query: 421 WFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPHEF 480
           WF TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLY NP+VNEAAVVARP EF
Sbjct: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501

Query: 481 WGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKYLL 540
           WGETPCAFVSLREGLT+ PTEEEIIEYC+GKL  FM PKTVVFM +LPKTSTGKIQK+LL
Sbjct: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561

Query: 541 KEMAMSMG 549
           +EMA SMG
Sbjct: 562 REMAKSMG 569

BLAST of CmaCh04G022590 vs. NCBI nr
Match: gi|590656928|ref|XP_007034413.1| (Acyl activating enzyme 5 [Theobroma cacao])

HSP 1 Score: 826.6 bits (2134), Expect = 2.5e-236
Identity = 391/550 (71.09%), Postives = 464/550 (84.36%), Query Frame = 1

Query: 1   MEHLKPRPANSSPLTPIGFLERAATIYADSPSIIYGNTTYTWSDTHRRCLQLASSLSSIG 60
           ME LKPRPA+SSPLTP+GFLERAAT+Y D  SIIY NT+YTWSDTHRRCLQ+ASSLSS G
Sbjct: 51  MEELKPRPASSSPLTPLGFLERAATVYGDCTSIIYNNTSYTWSDTHRRCLQIASSLSSTG 110

Query: 61  IQRGHVVSVISPNTPPMYELHFAVPMTGAILNTINTRLDARTISILLCHSESKLIFVDRA 120
           I+RGHVVSV++PN P MYELHFAVPMTGA+LN+INTRLDAR +SILL H ESKL+FVD  
Sbjct: 111 IKRGHVVSVLAPNIPAMYELHFAVPMTGAVLNSINTRLDARIVSILLRHCESKLLFVDTL 170

Query: 121 FCNLIHNALDLFPPEINRPMLVLVADDAAAEESS--SSVLIEGNFVDSYEGMIRKGDSEF 180
             +L   A+ LFPP    P+LVL+ DD+A  ESS  SS  ++  F  +YE +++KGD +F
Sbjct: 171 SRSLALEAISLFPPNTKPPLLVLIGDDSADAESSDSSSSTVDSRFCCTYESLVKKGDPDF 230

Query: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGSFIVTLDMLLEWGVPKQSVYLWTLPMF 240
           +W+RP SEW+PIVLNYTSGTTSSPKGVVH HRG+F++TLD L++WGVPKQ VYLWTLPMF
Sbjct: 231 KWVRPQSEWNPIVLNYTSGTTSSPKGVVHSHRGTFMMTLDSLIDWGVPKQPVYLWTLPMF 290

Query: 241 HANGWSFSWGMAAVGGTNICIRKFDAVLVYSLIRRHHVTHLCGAPVVLNMLTNSPDIRPL 300
           HANGWSF+WG+AAVGG N+C+RKFDA ++YSLI++H VTH+CGAPVVL+ML+N P+I+PL
Sbjct: 291 HANGWSFTWGIAAVGGINVCVRKFDAPIIYSLIKKHGVTHMCGAPVVLSMLSNFPEIKPL 350

Query: 301 DWPVKILTAGAPVPATILFRIESLGFDVTHGYGLTETGGTVVCCSWKAEWDQLPAVERAR 360
             PVKILTAGAP PA +LFR E+LGF V+HGYGLTETGG VV C+WK  W++LPA ERAR
Sbjct: 351 QSPVKILTAGAPPPAAVLFRTEALGFIVSHGYGLTETGGLVVSCAWKRGWNKLPATERAR 410

Query: 361 LKSRQGVRTVATPAVDVVDLETGESVARDGVSMGEIVLRVGSLMLGYFKDLEATAKSMTE 420
           LK+RQGVRT+     DVVD E+G SV RDG ++GEIVLR   +MLGY KD   T K M E
Sbjct: 411 LKARQGVRTLGMTEADVVDTESGLSVKRDGSTLGEIVLRGACIMLGYLKDPNGTNKCMKE 470

Query: 421 DGWFLTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYANPSVNEAAVVARPH 480
           +GWF TGDVGVMHPDGY+EIKDRSKDVIISGGENLSSVEVES+LY +P++ EAAVVARP 
Sbjct: 471 NGWFFTGDVGVMHPDGYMEIKDRSKDVIISGGENLSSVEVESILYNHPAIIEAAVVARPD 530

Query: 481 EFWGETPCAFVSLREGLTQQPTEEEIIEYCRGKLPRFMAPKTVVFMTELPKTSTGKIQKY 540
           ++WGETPCAFVSL+ GLTQ+P+E+EIIEYCR KLP +M PKTVVF  ELPKTSTGKIQK+
Sbjct: 531 DYWGETPCAFVSLKAGLTQKPSEKEIIEYCRAKLPHYMVPKTVVFKEELPKTSTGKIQKF 590

Query: 541 LLKEMAMSMG 549
           LL+E+A  MG
Sbjct: 591 LLREIAKGMG 600