BLAST of CmaCh04G018870 vs. Swiss-Prot
Match:
PME45_ARATH (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=PME45 PE=2 SV=1)
HSP 1 Score: 589.0 bits (1517), Expect = 6.1e-167
Identity = 311/612 (50.82%), Postives = 408/612 (66.67%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
M FQDFD+I ER ER RKFR+R+I+ VV V+ I F V+ + + K
Sbjct: 1 MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTY--ENKTQEQGK 60
Query: 61 TSN---KDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKA 120
T+N KD +K S + A +K+I +CNST YK C++ LK+ K
Sbjct: 61 TTNNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKK 120
Query: 121 DPKSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMS 180
D +DP+ L+K AI A ++ K+ +LK ++K A CK+L+++A EEL S
Sbjct: 121 DTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTS 180
Query: 181 MTAVSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTS 240
M ++ K PDL++WLSAVMSY ETCVDGF +G K+K E+ K S + LTS
Sbjct: 181 MKRINDSEVNNFA-KIVPDLDSWLSAVMSYQETCVDGFEEG-KLKTEIRKNFNSSQVLTS 240
Query: 241 NSMAMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTP 300
NS+AMI + + S R + D + SW+ + ERRMLK A P
Sbjct: 241 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLK-AVDVKALKP 300
Query: 301 NVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQ 360
N VA+DGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK NVTM GDGSQ
Sbjct: 301 NATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQ 360
Query: 361 KSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFL 420
K++++GNK+ A +RTF TA+FVA G+GF AQ++GFRNTAGPE HQAVA RVQ+DR++FL
Sbjct: 361 KTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFL 420
Query: 421 NCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTA 480
NCRF+GYQDTLYA HR +YRSC+I GT+DFIFGDAAAIFQNC++ ++K L Q+N VTA
Sbjct: 421 NCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTA 480
Query: 481 QGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPD 540
QGR D+ +TT V+ C + ++ L+PVK+QFKSYLGRPWK +SRT+VMESTIED+I P
Sbjct: 481 QGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPV 540
Query: 541 GWLAW-KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWI 600
GWL W + DFA+ TL YAE+ N+GP T RVKWPG +V++KEEA KFT+G FL+ +WI
Sbjct: 541 GWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGEWI 600
Query: 601 QSTSAPVHVGLF 609
Q+ +PV +GL+
Sbjct: 601 QAIGSPVKLGLY 607
BLAST of CmaCh04G018870 vs. Swiss-Prot
Match:
PME13_ARATH (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=PME13 PE=2 SV=2)
HSP 1 Score: 560.8 bits (1444), Expect = 1.8e-158
Identity = 303/624 (48.56%), Postives = 406/624 (65.06%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSS-- 60
M FQDFD+I ER R RKFR+R+I+ V V++ I F V+ +
Sbjct: 1 MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60
Query: 61 -SKTSNKDGKS--------KKHPKSNTDTD----TGTNAKDASSAEKMIVMICNSTDYKE 120
+K NK G KK P T + + + +K+I +C+ST Y +
Sbjct: 61 QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120
Query: 121 KCESILKDGLKADPKSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKV 180
CE LK+ +P +K AI A ++ L+K +LK ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180
Query: 181 LMEDAVEELEMSMTAVSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEM 240
L+EDA EE S+ ++ EK PDL +WLSAVMSY ETC+DGF +G+ +K+E+
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGN-LKSEV 240
Query: 241 EKIGKSGKELTSNSMAMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRM 300
+ S + LTSNS+A+I E+ P R L D++PSW+ +++RRM
Sbjct: 241 KTSVNSSQVLTSNSLALIKTFT------ENLSPVMKVVERHLL---DDIPSWVSNDDRRM 300
Query: 301 LKGATAGDKPTPNVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKK 360
L+ PN VA+DGSG+F TIN+AL AMP KY+GRY+IY+K+GIY+E V + KK
Sbjct: 301 LRAVDV-KALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKK 360
Query: 361 MVNVTMYGDGSQKSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAV 420
N+TM GDGSQK++++GNK+ A +RTF TA+FVA G+GF AQ++GFRNTAGPE HQAV
Sbjct: 361 KANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAV 420
Query: 421 AARVQADRAIFLNCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVK 480
A RVQ+DR+IFLNCRF+GYQDTLYA HR +YRSC+I GTIDFIFGDAAAIFQNCN+ ++
Sbjct: 421 AIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIR 480
Query: 481 KPLDNQQNIVTAQGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIV 540
K L Q+N VTAQGR D+ +TT V+ CKI A++ L+PVK ++KSYLGRPWK YSRTI+
Sbjct: 481 KGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTII 540
Query: 541 MESTIEDIIHPDGWLAW-KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAK 600
MES IE++I P GWL W + DFA+ TLYYAE+NN+G T RVKWPG KVI+KEEA
Sbjct: 541 MESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALN 600
Query: 601 FTIGSFLEVDWIQSTSAPVHVGLF 609
+T+G FL+ DWI ++ +PV +GL+
Sbjct: 601 YTVGPFLQGDWISASGSPVKLGLY 612
BLAST of CmaCh04G018870 vs. Swiss-Prot
Match:
PME58_ARATH (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=PME58 PE=2 SV=1)
HSP 1 Score: 458.4 bits (1178), Expect = 1.2e-127
Identity = 246/541 (45.47%), Postives = 341/541 (63.03%), Query Frame = 1
Query: 82 TNAKDASSAEKMIVM-----------ICNSTDYKEKCESILKDGLKADPKSSDPKELIKL 141
T +++ S +EK++ + +C TDYKE C + L +KA P S+ P +LIKL
Sbjct: 32 TTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKL 91
Query: 142 AITAAAAEVKSALKKA----TNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQ 201
++ ++KKA T + KGA E C+ LM DA ++L+ + +
Sbjct: 92 GFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSI 151
Query: 202 GKLTEKATPDLNNWLSAVMSYHETCVDGFPD-GSKMKAEMEKIGKSGKELTSNSMAMISQ 261
++ E DL WLS ++Y +TC+D F + SK+ +M+KI K+ +ELTSN +AMI+
Sbjct: 152 PQI-EDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITN 211
Query: 262 VATFFSKFESEG-PGE-GAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVVVAQ 321
++ +F G G+ G R+ L +D +PSW+ N RR++ AT G NVVVA
Sbjct: 212 ISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSWVGPNTRRLM--ATKGGVKA-NVVVAH 271
Query: 322 DGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISG 381
DGSG +KTINEAL A+P +VIY+K+G+Y E V +TKKM +VT GDG K+ I+G
Sbjct: 272 DGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITG 331
Query: 382 NKNFADG-VRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFD 441
+ N+ G V+T+ TA+ GD F A+ IGF NTAGPE HQAVA RV AD A+F NC+ D
Sbjct: 332 SLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQID 391
Query: 442 GYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSD 501
GYQDTLY +HR F+R C ++GT+DFIFGD + QNCN++V+KP+ +Q ++TAQGRSD
Sbjct: 392 GYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSD 451
Query: 502 RHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAW 561
+ E+T +VLQ C I + PVKS K+YLGRPWKE+SRTI+M +TI+D+I P GWL W
Sbjct: 452 KRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPW 511
Query: 562 KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSA 603
GDFAL TLYYAE+ N GPG+ RVKWPG K + ++A +FT FL + WI
Sbjct: 512 NGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRV 565
BLAST of CmaCh04G018870 vs. Swiss-Prot
Match:
PME28_ARATH (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=PME28 PE=2 SV=1)
HSP 1 Score: 441.4 bits (1134), Expect = 1.6e-122
Identity = 246/597 (41.21%), Postives = 352/597 (58.96%), Query Frame = 1
Query: 16 ERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSKTSNKDGKSKKHPKSN 75
E A++ ++ VII++ +I +++A + + V+ + ++
Sbjct: 8 EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------------------- 67
Query: 76 TDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPKSSDPKELIKLAITAA 135
++ +++ K I +C TDYKE CE L+ K +SDP EL+K A A
Sbjct: 68 ---------EEITTSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNAT 127
Query: 136 AAEVKSALKKATNL--KFTTPEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQGKLTEKA 195
++ KK+ + P K A + CK LM+ A+ EL S + K K+ E A
Sbjct: 128 MKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDE-A 187
Query: 196 TPDLNNWLSAVMSYHETCVDGFPDGSKMKAE-MEKIGKSGKELTSNSMAMISQVATFFSK 255
L WLSA +S+ +TC+DGF E ++K K+ +LT N +AM+++++ + +
Sbjct: 188 LVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQ 247
Query: 256 FESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVVVAQDGSGNFKTI 315
+ RRL+ Q E PSWMD+ RR+L + KP ++VVAQDGSG +KTI
Sbjct: 248 MQIP-----EMNSRRLLSQ-EFPSWMDARARRLLNAPMSEVKP--DIVVAQDGSGQYKTI 307
Query: 316 NEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGNKNFADGVR 375
NEAL +P K + +V+++KEGIY+E V + + M ++ GDG K++ISG+K++ DG+
Sbjct: 308 NEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGIT 367
Query: 376 TFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGYQDTLYAQA 435
T++TA+ +GD F A+ I F NTAG KHQAVA RV AD +IF NC+FDGYQDTLYA +
Sbjct: 368 TYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHS 427
Query: 436 HRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSDRHETTAIVLQ 495
HR FYR C I+GTIDF+FGDAAA+FQNC ++V+KPL NQ +TA GR D E+T VLQ
Sbjct: 428 HRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQ 487
Query: 496 RCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAWKGDFALKTLY 555
C I+ + VK Q K+YLGRPWKEYSRTI+M + I D + P+GW W G+F L TL+
Sbjct: 488 GCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLF 547
Query: 556 YAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSAPVHVGLF 609
Y+E N GPGA RV WPG K + EE KFT +++ D WI P +GLF
Sbjct: 548 YSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563
BLAST of CmaCh04G018870 vs. Swiss-Prot
Match:
PME23_ARATH (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=PME23 PE=2 SV=3)
HSP 1 Score: 437.6 bits (1124), Expect = 2.3e-121
Identity = 238/521 (45.68%), Postives = 326/521 (62.57%), Query Frame = 1
Query: 92 KMIVMICNSTDYKEKCESILKDGLKADPKSSDPKELIKLAITAAAAEVKSALKKAT-NLK 151
K + +C TD+K+ C + L + A P S DP +LIKL + +L+KA+ ++K
Sbjct: 49 KAVQAVCAPTDFKDTCVNSL---MGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIK 108
Query: 152 FTT---PEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQGKLTEKATPDLNNWLSAVMSY 211
PE KGAFE C+ LM DA+++L+ M +Q E DL WLS +++
Sbjct: 109 AKADKNPEAKGAFELCEKLMIDAIDDLKKCMDHGFSVDQ---IEVFVEDLRVWLSGSIAF 168
Query: 212 HETCVDGFPD-GSKMKAEMEKIGKSGKELTSNSMAMISQVATFFSKFESEGPGEGAA--T 271
+TC+D F + S + +M KI K+ +EL+SNS+AM+++++T G A
Sbjct: 169 QQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA 228
Query: 272 RRRLMDQDELPSWMDSNERRMLKGATAGDKPTP-NVVVAQDGSGNFKTINEALAAMPAKY 331
R+ L +D +P+W+ RR++ G P N VVAQDG+G FKTI +AL A+P
Sbjct: 229 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 288
Query: 332 DGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGNKNFADG-VRTFQTASFVAL 391
++I++KEGIY+E V +TKKM +VT GDG K++I+G+ NF G V+TF TA+
Sbjct: 289 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 348
Query: 392 GDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGYQDTLYAQAHRHFYRSCLI 451
GD F A+ IG NTAGPE QAVA RV AD A+F +C+ DG+QDTLY +HR FYR C +
Sbjct: 349 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 408
Query: 452 AGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSDRHETTAIVLQRCKILADKTL 511
+GT+DFIFGDA I QNC ++V+KP Q +VTAQGRS+ E+T +VL C I D
Sbjct: 409 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 468
Query: 512 EPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAWKGDFALKTLYYAEFNNEGPG 571
P+KS K+YLGRPWKE+SRTI+M++TI+D+I P GWL W GDFALKTLYYAE N GPG
Sbjct: 469 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 528
Query: 572 AKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSAP 603
+ RVKWPG K + ++A +T FL D WI T P
Sbjct: 529 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVP 563
BLAST of CmaCh04G018870 vs. TrEMBL
Match:
A0A0A0KX33_CUCSA (Pectinesterase OS=Cucumis sativus GN=Csa_5G630740 PE=4 SV=1)
HSP 1 Score: 940.3 bits (2429), Expect = 1.2e-270
Identity = 474/608 (77.96%), Postives = 537/608 (88.32%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRR ERARKFR+RVII VV A+VI+L++AAAVF+QVS + S++ + S
Sbjct: 1 MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
+S+ + K P++ K S EKMI MICNSTDYK KCES LKDG+ DP
Sbjct: 61 SSSGNSKQPLPPET----------KQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPN 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
SSDPK+LIKLAI+AAA EVKSA+KKA+ F TPEEKGAFEDCKVL+EDA+EELEMSM+
Sbjct: 121 SSDPKDLIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSE 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
V+KKN GKLT K TP+LNNWLSAVMSYHETCVDGFP+G KMK+++EK+ K+GKELTSNS+
Sbjct: 181 VNKKNMGKLTAKTTPNLNNWLSAVMSYHETCVDGFPEG-KMKSDIEKVVKAGKELTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVV 300
AMISQVA+FFS FE EGAA+RRRLM + +P+WMD N+RRMLKGA AG+KP PNVV
Sbjct: 241 AMISQVASFFSTFEMP---EGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVV 300
Query: 301 VAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSM 360
VA+DGSG FKTINEALAAMPAKYDGRYVIY+KEGIY+E VVITKKMVNVTMYGDGSQKSM
Sbjct: 301 VAKDGSGEFKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSM 360
Query: 361 ISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCR 420
ISG+KNF DGVRTFQTA+FVALG+GF QAIGFRN AGPEKHQAVAARVQADRAIF+NCR
Sbjct: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCR 420
Query: 421 FDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGR 480
F+GYQDTLY QAHR FYRSCLI GTIDFIFGDAAAIFQNCNMM++KPLDNQQN+VTAQGR
Sbjct: 421 FEGYQDTLYTQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGR 480
Query: 481 SDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWL 540
+D+HETT IVLQ CKIL DKTLEPVKSQFKSYLGRPWKE+SRTIVMESTIED+IHPDGW+
Sbjct: 481 TDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWM 540
Query: 541 AWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTS 600
AW+GDFALKTLYYAEFNN+GPGAKT+ RVKWPG+KVIDK+EAAKFTIG+FLE+DWI+STS
Sbjct: 541 AWEGDFALKTLYYAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTS 594
Query: 601 APVHVGLF 609
APVHVGLF
Sbjct: 601 APVHVGLF 594
BLAST of CmaCh04G018870 vs. TrEMBL
Match:
A0A067KCM7_JATCU (Pectinesterase OS=Jatropha curcas GN=JCGZ_07552 PE=4 SV=1)
HSP 1 Score: 730.7 bits (1885), Expect = 1.4e-207
Identity = 377/611 (61.70%), Postives = 469/611 (76.76%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRRLER +KFR+R+ I + +++++I VF +S + ++ SK
Sbjct: 1 MVFQDFDQISERRRLEREQKFRKRITIAAITIVLLLVIIGCGVFAIIS---ENKNTKRSK 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
S+++ K +AKD + +++I ICN TDYK+ C S L+ +K DP
Sbjct: 61 ESSQESKG--------------SAKDVAQVDRVIRRICNGTDYKDTCVSSLESEVKKDPS 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
S++PK +++LAI A E+ LKKA++ KF +P+EK AFEDCKVL++DA EEL+ S+T
Sbjct: 121 SANPKAILRLAIEATDDEMGVVLKKASSFKFESPQEKAAFEDCKVLIKDAKEELKESLTH 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
+ KL E P+LNN LSAVMSY +TC+DGFPDG K+K++MEK + KELTSNS+
Sbjct: 181 AGVDIK-KLAENE-PELNNLLSAVMSYQQTCIDGFPDG-KLKSDMEKTFNASKELTSNSL 240
Query: 241 AMISQVATFFSKF-ESEGPGEGAATRRRL--MDQDELPSWMDSNERRMLKGATAGDKPTP 300
AM+S + +F S F E AT+ L MD+DELPSW+ + +RR+LK A DKPTP
Sbjct: 241 AMVSSLTSFLSSFGEINHSRRLLATKPNLSSMDKDELPSWISNEDRRILK-ADDSDKPTP 300
Query: 301 NVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQ 360
NV+VA+DGSG FKTI+EAL AMP KY GRY+IY+K G+Y+E V + KKM N+TMYGDGSQ
Sbjct: 301 NVIVAKDGSGKFKTISEALKAMPKKYKGRYIIYVKAGVYDEVVTVEKKMENITMYGDGSQ 360
Query: 361 KSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFL 420
KS+I+GNKNF DGV+TF+TASFVALG+GF A+AIGFRNTAGPEKHQAVAARVQADR IFL
Sbjct: 361 KSIITGNKNFKDGVQTFRTASFVALGNGFMAKAIGFRNTAGPEKHQAVAARVQADRTIFL 420
Query: 421 NCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTA 480
NCRF+GYQDTLY Q HR +YRSC+IAGTIDFIFGDAAAI QNC ++++KPL+NQQNIVTA
Sbjct: 421 NCRFEGYQDTLYVQTHRQYYRSCVIAGTIDFIFGDAAAILQNCQIVIRKPLENQQNIVTA 480
Query: 481 QGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPD 540
QGR D HETT IVLQ C+I DK+L P K++FKSYLGRPWKEYSRTIVMESTIED+IHPD
Sbjct: 481 QGRIDPHETTGIVLQNCRIQPDKSLVPDKAKFKSYLGRPWKEYSRTIVMESTIEDLIHPD 540
Query: 541 GWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQ 600
GWLAW+G+ LKTLYYAEFNN+GPGA T+ RVKWPG+ VIDKEEA K+TI F++ DWI
Sbjct: 541 GWLAWEGEKGLKTLYYAEFNNKGPGASTKARVKWPGYHVIDKEEANKYTIEPFIQGDWIN 590
Query: 601 STSAPVHVGLF 609
APVH GLF
Sbjct: 601 DMGAPVHFGLF 590
BLAST of CmaCh04G018870 vs. TrEMBL
Match:
M5VTB0_PRUPE (Pectinesterase OS=Prunus persica GN=PRUPE_ppa023802mg PE=4 SV=1)
HSP 1 Score: 711.1 bits (1834), Expect = 1.2e-201
Identity = 370/612 (60.46%), Postives = 468/612 (76.47%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
M FQDFDQISERR+ ERARK R+R+II VV+ ++IL+ A + ++ K
Sbjct: 1 MAFQDFDQISERRKAERARKMRKRIIIAVVVVVLLILIAVGAYLLLNKLNSKKGNAKQDK 60
Query: 61 TSN-KDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADP 120
T+N K +K PK++ K+ + EK++ +C +TDY++KCESI+ A
Sbjct: 61 TANSKPAPAKAQPKNDQ-----AKKKEPTKGEKIMKQMCGATDYEDKCESIIGKAKGA-- 120
Query: 121 KSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMT 180
S PKE IK AI+AA+ E + A K++ L F +PEEKGAFEDCKVL EDA++EL +++
Sbjct: 121 --SKPKEFIKTAISAASDEARIAYSKSSELTFNSPEEKGAFEDCKVLFEDAMDELGDAIS 180
Query: 181 AVSKKN-QGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSN 240
+ GK+ T LN WLSAV+SY +TCVDGFPDG K+K+++EK+ ++ KE TSN
Sbjct: 181 QIGNTTASGKI---RTGLLNTWLSAVISYQQTCVDGFPDG-KLKSDLEKMLQASKEFTSN 240
Query: 241 SMAMISQVATFFSKFESEGPGEGAATRRRLMDQ--DELPSWMDSNERRMLKGATAGDKPT 300
S+AM+S S+F+ + +RRL+ Q D P+WM ERR+LK +KPT
Sbjct: 241 SLAMLS----LLSQFQLPVTAAVSGAKRRLLAQNKDGFPTWMSHEERRVLK--KNDEKPT 300
Query: 301 PNVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGS 360
PNV VA+DGSGNFKTI+EALAAMPAKY+GRY+IY+K G+Y+E V++TKKM NVT+YGDGS
Sbjct: 301 PNVTVAKDGSGNFKTISEALAAMPAKYEGRYIIYVKGGVYDETVIVTKKMPNVTIYGDGS 360
Query: 361 QKSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIF 420
QKS+I+GNKNFADGVRTFQTASF ALG+GF A+++GFRNTAGPEKHQAVAARVQADRAIF
Sbjct: 361 QKSIITGNKNFADGVRTFQTASFAALGEGFMAKSMGFRNTAGPEKHQAVAARVQADRAIF 420
Query: 421 LNCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVT 480
LNCRF+GYQDTLYAQ HR FY+SC+I+GT+DFIFGDAAAIFQNC + V+KP++NQQNIVT
Sbjct: 421 LNCRFEGYQDTLYAQTHRQFYKSCVISGTVDFIFGDAAAIFQNCLIYVRKPMENQQNIVT 480
Query: 481 AQGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHP 540
AQGR+D+ ETT IVLQ CKI+ DK LEPVKSQFK+YLGRPWKE+SRTIVM STIED+IHP
Sbjct: 481 AQGRTDKRETTGIVLQNCKIMPDKDLEPVKSQFKTYLGRPWKEFSRTIVMGSTIEDLIHP 540
Query: 541 DGWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-W 600
DGW W+GDFALKTLYYAE+NN+GPGAKT+ RVKW G+KVIDK+EA K+T+G FL+ + W
Sbjct: 541 DGWTPWQGDFALKTLYYAEYNNKGPGAKTDNRVKWSGYKVIDKQEAMKYTVGPFLKGNAW 593
Query: 601 IQSTSAPVHVGL 608
++ PV GL
Sbjct: 601 LRVKGVPVRFGL 593
BLAST of CmaCh04G018870 vs. TrEMBL
Match:
B9RR24_RICCO (Pectinesterase OS=Ricinus communis GN=RCOM_0708480 PE=4 SV=1)
HSP 1 Score: 710.7 bits (1833), Expect = 1.5e-201
Identity = 363/611 (59.41%), Postives = 459/611 (75.12%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRRLER +KFR+RV I V +++++AA VF +S +++ S +
Sbjct: 1 MVFQDFDQISERRRLERQQKFRKRVTIAAVAVFALLVVVAAGVFAIIS---NNNHKQSQQ 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
+K +S+K S + S K+I +CN+T YKE C + LK ++ DP
Sbjct: 61 QHSKSTQSQKPDNS-----------EVSHLGKVIDNVCNATTYKETCHTSLKKAVEKDPS 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
S+ PK+++KLAI + E L+K + KF +P EK AFEDCK L++DA EEL S+++
Sbjct: 121 SAHPKDVLKLAIGSTEDEFARILEKVKSFKFESPREKAAFEDCKELIDDAKEELNKSISS 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
+ GKL K DLNNWLSAVMSY +TC+DGFP+G K+K++MEK K KELTSNS+
Sbjct: 181 AGG-DTGKLL-KNEADLNNWLSAVMSYQQTCIDGFPEG-KLKSDMEKTFKEAKELTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRR---LMDQDELPSWMDSNERRMLKGATAGDKPTP 300
AM+S++ F + F P + +D +PSW+ +RR+LKG+ GDKPTP
Sbjct: 241 AMVSELTAFLTAFSVPKPSRRLLAKESNTSSFGEDGIPSWISPEDRRILKGSD-GDKPTP 300
Query: 301 NVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQ 360
NV VA+DGSG FKTI++ALAAMP KY GRYVIY+K GIY+E V +TK MVNVT+YGDGSQ
Sbjct: 301 NVTVAKDGSGQFKTISDALAAMPEKYQGRYVIYVKAGIYDETVTVTKNMVNVTIYGDGSQ 360
Query: 361 KSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFL 420
KS+++G+KNFADGV+TF+TA+F ALGDGF A+A+GFRNTAGP+KHQAVA RVQADR+IFL
Sbjct: 361 KSIVTGSKNFADGVQTFRTATFAALGDGFIAKAMGFRNTAGPQKHQAVAVRVQADRSIFL 420
Query: 421 NCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTA 480
NCRF+GYQDTLYAQ HR FYRSC+I+GTIDFIFGDA AIFQNC ++V+KP++NQQNIVTA
Sbjct: 421 NCRFEGYQDTLYAQTHRQFYRSCVISGTIDFIFGDATAIFQNCLILVRKPMENQQNIVTA 480
Query: 481 QGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPD 540
QGR D HETT IV+Q C+I DK L P K+ KSYLGRPWK+YSRTIVMESTIED IHPD
Sbjct: 481 QGRIDSHETTGIVIQNCRIQPDKDLIPAKATVKSYLGRPWKDYSRTIVMESTIEDFIHPD 540
Query: 541 GWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQ 600
GWLAW+G+ LKTLYYAEFNN+GPG+KT+ RVKWPG+ VID++EA K+T+ FL+ DWI
Sbjct: 541 GWLAWEGEKGLKTLYYAEFNNKGPGSKTDARVKWPGYHVIDQQEANKYTVKPFLQGDWIT 593
Query: 601 STSAPVHVGLF 609
+ APVH GLF
Sbjct: 601 AAGAPVHFGLF 593
BLAST of CmaCh04G018870 vs. TrEMBL
Match:
U5G7D7_POPTR (Pectinesterase OS=Populus trichocarpa GN=POPTR_0006s13690g PE=4 SV=1)
HSP 1 Score: 706.4 bits (1822), Expect = 2.9e-200
Identity = 359/615 (58.37%), Postives = 462/615 (75.12%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQ+SERR+LER +K R+++II V + +++ A VF VS SS S+
Sbjct: 1 MVFQDFDQLSERRKLERQQKLRKKIIIGSVSSIAFFVIVGAGVFALVSNHNISSPGSNGG 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
+ + T + +AK S ++I +CN+T Y++ C++ L+ G++ DP
Sbjct: 61 SPS------------TVSQPVESAKPTSHVARVIKTVCNATTYQDTCQNTLEKGMRKDPS 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
S PK+L+K+AI AA E++ LKKA++ KF P EK AF+DC L+EDA EEL+ +
Sbjct: 121 SVQPKDLLKIAIKAADKEIEKVLKKASSFKFDKPREKAAFDDCLELIEDAKEELKHCIDR 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
V + GKLT+ A PDLNNWLSAVMSY +TC+DGFP+G K+K++MEK K+ +ELTSNS+
Sbjct: 181 VGN-DIGKLTKNA-PDLNNWLSAVMSYQQTCIDGFPEG-KLKSDMEKTFKAARELTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRRL--------MDQDELPSWMDSNERRMLKGATAG 300
AM+S +A+F F G RR L +D+D LP W +RR+LKGA
Sbjct: 241 AMVSSLASFLKNFSFSG----TLNRRLLAEEYNSPSLDKDGLPGWTSHEDRRILKGANQ- 300
Query: 301 DKPTPNVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMY 360
DKP P+V VA+DGSG+FKTI+EALAAMPAKY+GRYVI++K+GIY+E V +TKKMVN+TMY
Sbjct: 301 DKPKPHVTVAKDGSGDFKTISEALAAMPAKYEGRYVIFVKQGIYDETVTVTKKMVNITMY 360
Query: 361 GDGSQKSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQAD 420
GDGSQK++++GNKNFADGV+TF+TA+F LG+GF +A+GFRNTAGPEKHQAVA RVQAD
Sbjct: 361 GDGSQKTIVTGNKNFADGVQTFRTATFAVLGEGFLCKAMGFRNTAGPEKHQAVAIRVQAD 420
Query: 421 RAIFLNCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQ 480
RAIFLNCRF+GYQDTLYAQ HR FYRSC+I GT+DFIFGDAAAIFQNC + V+KPL+NQQ
Sbjct: 421 RAIFLNCRFEGYQDTLYAQTHRQFYRSCVITGTVDFIFGDAAAIFQNCLITVRKPLENQQ 480
Query: 481 NIVTAQGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIED 540
NIVTAQGR D HETT IVLQ C+I DK L PVK++ +SYLGRPWKE+SRT++M+STI D
Sbjct: 481 NIVTAQGRIDGHETTGIVLQNCRIEPDKGLVPVKTKIRSYLGRPWKEFSRTVIMDSTIGD 540
Query: 541 IIHPDGWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLE 600
IHPDGWL W+GDF LKTLYYAE+NN+G GA+T R+KW G+ +I KEEA KFT+ +F +
Sbjct: 541 FIHPDGWLPWQGDFGLKTLYYAEYNNKGIGAQTNARIKWRGYHIIKKEEAMKFTVETFYQ 595
Query: 601 VDWIQSTSAPVHVGL 608
VDWI +T +PV +GL
Sbjct: 601 VDWISATGSPVRLGL 595
BLAST of CmaCh04G018870 vs. TAIR10
Match:
AT4G33230.1 (AT4G33230.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 589.0 bits (1517), Expect = 3.4e-168
Identity = 311/612 (50.82%), Postives = 408/612 (66.67%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
M FQDFD+I ER ER RKFR+R+I+ VV V+ I F V+ + + K
Sbjct: 1 MAFQDFDKIQERVNAERKRKFRKRIILGVVSVLVVAAAIIGGAFAYVTY--ENKTQEQGK 60
Query: 61 TSN---KDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKA 120
T+N KD +K S + A +K+I +CNST YK C++ LK+ K
Sbjct: 61 TTNNKSKDSPTKSESPSPKPPSSAAQTVKAGQVDKIIQTLCNSTLYKPTCQNTLKNETKK 120
Query: 121 DPKSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMS 180
D +DP+ L+K AI A ++ K+ +LK ++K A CK+L+++A EEL S
Sbjct: 121 DTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKTENKDDKDAIAQCKLLVDEAKEELGTS 180
Query: 181 MTAVSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTS 240
M ++ K PDL++WLSAVMSY ETCVDGF +G K+K E+ K S + LTS
Sbjct: 181 MKRINDSEVNNFA-KIVPDLDSWLSAVMSYQETCVDGFEEG-KLKTEIRKNFNSSQVLTS 240
Query: 241 NSMAMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTP 300
NS+AMI + + S R + D + SW+ + ERRMLK A P
Sbjct: 241 NSLAMIKSLDGYLSSVPKVKTRLLLEARSSAKETDHITSWLSNKERRMLK-AVDVKALKP 300
Query: 301 NVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQ 360
N VA+DGSGNF TIN AL AMPAKY GRY IY+K GIY+E+V+I KK NVTM GDGSQ
Sbjct: 301 NATVAKDGSGNFTTINAALKAMPAKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQ 360
Query: 361 KSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFL 420
K++++GNK+ A +RTF TA+FVA G+GF AQ++GFRNTAGPE HQAVA RVQ+DR++FL
Sbjct: 361 KTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFL 420
Query: 421 NCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTA 480
NCRF+GYQDTLYA HR +YRSC+I GT+DFIFGDAAAIFQNC++ ++K L Q+N VTA
Sbjct: 421 NCRFEGYQDTLYAYTHRQYYRSCVIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTA 480
Query: 481 QGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPD 540
QGR D+ +TT V+ C + ++ L+PVK+QFKSYLGRPWK +SRT+VMESTIED+I P
Sbjct: 481 QGRVDKFQTTGFVIHNCTVAPNEDLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPV 540
Query: 541 GWLAW-KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWI 600
GWL W + DFA+ TL YAE+ N+GP T RVKWPG +V++KEEA KFT+G FL+ +WI
Sbjct: 541 GWLRWQETDFAIDTLSYAEYKNDGPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGEWI 600
Query: 601 QSTSAPVHVGLF 609
Q+ +PV +GL+
Sbjct: 601 QAIGSPVKLGLY 607
BLAST of CmaCh04G018870 vs. TAIR10
Match:
AT2G26450.1 (AT2G26450.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 560.8 bits (1444), Expect = 1.0e-159
Identity = 303/624 (48.56%), Postives = 406/624 (65.06%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSS-- 60
M FQDFD+I ER R RKFR+R+I+ V V++ I F V+ +
Sbjct: 1 MAFQDFDKIQERVNANRKRKFRKRIIVGTVSLLVVVAAIVGGAFAYVAYEKRNEQQQQQQ 60
Query: 61 -SKTSNKDGKS--------KKHPKSNTDTD----TGTNAKDASSAEKMIVMICNSTDYKE 120
+K NK G KK P T + + + +K+I +C+ST Y +
Sbjct: 61 QAKNHNKSGSGNNVVKDSDKKSPSPPTPSQKAPVSAAQSVKPGQGDKIIQTLCSSTLYMQ 120
Query: 121 KCESILKDGLKADPKSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKV 180
CE LK+ +P +K AI A ++ L+K +LK ++K A E CK+
Sbjct: 121 ICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKTENQDDKDAIEQCKL 180
Query: 181 LMEDAVEELEMSMTAVSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEM 240
L+EDA EE S+ ++ EK PDL +WLSAVMSY ETC+DGF +G+ +K+E+
Sbjct: 181 LVEDAKEETVASLNKINVTEVNSF-EKVVPDLESWLSAVMSYQETCLDGFEEGN-LKSEV 240
Query: 241 EKIGKSGKELTSNSMAMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRM 300
+ S + LTSNS+A+I E+ P R L D++PSW+ +++RRM
Sbjct: 241 KTSVNSSQVLTSNSLALIKTFT------ENLSPVMKVVERHLL---DDIPSWVSNDDRRM 300
Query: 301 LKGATAGDKPTPNVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKK 360
L+ PN VA+DGSG+F TIN+AL AMP KY+GRY+IY+K+GIY+E V + KK
Sbjct: 301 LRAVDV-KALKPNATVAKDGSGDFTTINDALRAMPEKYEGRYIIYVKQGIYDEYVTVDKK 360
Query: 361 MVNVTMYGDGSQKSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAV 420
N+TM GDGSQK++++GNK+ A +RTF TA+FVA G+GF AQ++GFRNTAGPE HQAV
Sbjct: 361 KANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEGFMAQSMGFRNTAGPEGHQAV 420
Query: 421 AARVQADRAIFLNCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVK 480
A RVQ+DR+IFLNCRF+GYQDTLYA HR +YRSC+I GTIDFIFGDAAAIFQNCN+ ++
Sbjct: 421 AIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGTIDFIFGDAAAIFQNCNIFIR 480
Query: 481 KPLDNQQNIVTAQGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIV 540
K L Q+N VTAQGR D+ +TT V+ CKI A++ L+PVK ++KSYLGRPWK YSRTI+
Sbjct: 481 KGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPVKEEYKSYLGRPWKNYSRTII 540
Query: 541 MESTIEDIIHPDGWLAW-KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAK 600
MES IE++I P GWL W + DFA+ TLYYAE+NN+G T RVKWPG KVI+KEEA
Sbjct: 541 MESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGDTTSRVKWPGFKVINKEEALN 600
Query: 601 FTIGSFLEVDWIQSTSAPVHVGLF 609
+T+G FL+ DWI ++ +PV +GL+
Sbjct: 601 YTVGPFLQGDWISASGSPVKLGLY 612
BLAST of CmaCh04G018870 vs. TAIR10
Match:
AT5G49180.1 (AT5G49180.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 458.4 bits (1178), Expect = 7.0e-129
Identity = 246/541 (45.47%), Postives = 341/541 (63.03%), Query Frame = 1
Query: 82 TNAKDASSAEKMIVM-----------ICNSTDYKEKCESILKDGLKADPKSSDPKELIKL 141
T +++ S +EK++ + +C TDYKE C + L +KA P S+ P +LIKL
Sbjct: 32 TTSRNTSHSEKIVPVQIKTATTAVEAVCAPTDYKETCVNSL---MKASPDSTQPLDLIKL 91
Query: 142 AITAAAAEVKSALKKA----TNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQ 201
++ ++KKA T + KGA E C+ LM DA ++L+ + +
Sbjct: 92 GFNVTIRSIEDSIKKASVELTAKAANDKDTKGALELCEKLMNDATDDLKKCLDNFDGFSI 151
Query: 202 GKLTEKATPDLNNWLSAVMSYHETCVDGFPD-GSKMKAEMEKIGKSGKELTSNSMAMISQ 261
++ E DL WLS ++Y +TC+D F + SK+ +M+KI K+ +ELTSN +AMI+
Sbjct: 152 PQI-EDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLSQDMQKIFKTSRELTSNGLAMITN 211
Query: 262 VATFFSKFESEG-PGE-GAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVVVAQ 321
++ +F G G+ G R+ L +D +PSW+ N RR++ AT G NVVVA
Sbjct: 212 ISNLLGEFNVTGVTGDLGKYARKLLSAEDGIPSWVGPNTRRLM--ATKGGVKA-NVVVAH 271
Query: 322 DGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISG 381
DGSG +KTINEAL A+P +VIY+K+G+Y E V +TKKM +VT GDG K+ I+G
Sbjct: 272 DGSGQYKTINEALNAVPKANQKPFVIYIKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITG 331
Query: 382 NKNFADG-VRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFD 441
+ N+ G V+T+ TA+ GD F A+ IGF NTAGPE HQAVA RV AD A+F NC+ D
Sbjct: 332 SLNYYIGKVKTYLTATVAINGDNFTAKNIGFENTAGPEGHQAVALRVSADLAVFYNCQID 391
Query: 442 GYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSD 501
GYQDTLY +HR F+R C ++GT+DFIFGD + QNCN++V+KP+ +Q ++TAQGRSD
Sbjct: 392 GYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSD 451
Query: 502 RHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAW 561
+ E+T +VLQ C I + PVKS K+YLGRPWKE+SRTI+M +TI+D+I P GWL W
Sbjct: 452 KRESTGLVLQNCHITGEPAYIPVKSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPW 511
Query: 562 KGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSA 603
GDFAL TLYYAE+ N GPG+ RVKWPG K + ++A +FT FL + WI
Sbjct: 512 NGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIPPNRV 565
BLAST of CmaCh04G018870 vs. TAIR10
Match:
AT5G27870.1 (AT5G27870.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 441.4 bits (1134), Expect = 8.9e-124
Identity = 246/597 (41.21%), Postives = 352/597 (58.96%), Query Frame = 1
Query: 16 ERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSKTSNKDGKSKKHPKSN 75
E A++ ++ VII++ +I +++A + + V+ + ++
Sbjct: 8 EDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------------------- 67
Query: 76 TDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPKSSDPKELIKLAITAA 135
++ +++ K I +C TDYKE CE L+ K +SDP EL+K A A
Sbjct: 68 ---------EEITTSVKAIKDVCAPTDYKETCEDTLRKDAK---DTSDPLELVKTAFNAT 127
Query: 136 AAEVKSALKKATNL--KFTTPEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQGKLTEKA 195
++ KK+ + P K A + CK LM+ A+ EL S + K K+ E A
Sbjct: 128 MKQISDVAKKSQTMIELQKDPRAKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDE-A 187
Query: 196 TPDLNNWLSAVMSYHETCVDGFPDGSKMKAE-MEKIGKSGKELTSNSMAMISQVATFFSK 255
L WLSA +S+ +TC+DGF E ++K K+ +LT N +AM+++++ + +
Sbjct: 188 LVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQ 247
Query: 256 FESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVVVAQDGSGNFKTI 315
+ RRL+ Q E PSWMD+ RR+L + KP ++VVAQDGSG +KTI
Sbjct: 248 MQIP-----EMNSRRLLSQ-EFPSWMDARARRLLNAPMSEVKP--DIVVAQDGSGQYKTI 307
Query: 316 NEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGNKNFADGVR 375
NEAL +P K + +V+++KEGIY+E V + + M ++ GDG K++ISG+K++ DG+
Sbjct: 308 NEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGIT 367
Query: 376 TFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGYQDTLYAQA 435
T++TA+ +GD F A+ I F NTAG KHQAVA RV AD +IF NC+FDGYQDTLYA +
Sbjct: 368 TYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHS 427
Query: 436 HRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSDRHETTAIVLQ 495
HR FYR C I+GTIDF+FGDAAA+FQNC ++V+KPL NQ +TA GR D E+T VLQ
Sbjct: 428 HRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQ 487
Query: 496 RCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAWKGDFALKTLY 555
C I+ + VK Q K+YLGRPWKEYSRTI+M + I D + P+GW W G+F L TL+
Sbjct: 488 GCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLF 547
Query: 556 YAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSAPVHVGLF 609
Y+E N GPGA RV WPG K + EE KFT +++ D WI P +GLF
Sbjct: 548 YSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLF 563
BLAST of CmaCh04G018870 vs. TAIR10
Match:
AT3G06830.1 (AT3G06830.1 Plant invertase/pectin methylesterase inhibitor superfamily)
HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-122
Identity = 238/521 (45.68%), Postives = 326/521 (62.57%), Query Frame = 1
Query: 92 KMIVMICNSTDYKEKCESILKDGLKADPKSSDPKELIKLAITAAAAEVKSALKKAT-NLK 151
K + +C TD+K+ C + L + A P S DP +LIKL + +L+KA+ ++K
Sbjct: 49 KAVQAVCAPTDFKDTCVNSL---MGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIK 108
Query: 152 FTT---PEEKGAFEDCKVLMEDAVEELEMSMTAVSKKNQGKLTEKATPDLNNWLSAVMSY 211
PE KGAFE C+ LM DA+++L+ M +Q E DL WLS +++
Sbjct: 109 AKADKNPEAKGAFELCEKLMIDAIDDLKKCMDHGFSVDQ---IEVFVEDLRVWLSGSIAF 168
Query: 212 HETCVDGFPD-GSKMKAEMEKIGKSGKELTSNSMAMISQVATFFSKFESEGPGEGAA--T 271
+TC+D F + S + +M KI K+ +EL+SNS+AM+++++T G A
Sbjct: 169 QQTCMDSFGEIKSNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYA 228
Query: 272 RRRLMDQDELPSWMDSNERRMLKGATAGDKPTP-NVVVAQDGSGNFKTINEALAAMPAKY 331
R+ L +D +P+W+ RR++ G P N VVAQDG+G FKTI +AL A+P
Sbjct: 229 RKLLSTEDSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPKGN 288
Query: 332 DGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSMISGNKNFADG-VRTFQTASFVAL 391
++I++KEGIY+E V +TKKM +VT GDG K++I+G+ NF G V+TF TA+
Sbjct: 289 KVPFIIHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIE 348
Query: 392 GDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCRFDGYQDTLYAQAHRHFYRSCLI 451
GD F A+ IG NTAGPE QAVA RV AD A+F +C+ DG+QDTLY +HR FYR C +
Sbjct: 349 GDHFTAKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTV 408
Query: 452 AGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGRSDRHETTAIVLQRCKILADKTL 511
+GT+DFIFGDA I QNC ++V+KP Q +VTAQGRS+ E+T +VL C I D
Sbjct: 409 SGTVDFIFGDAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAY 468
Query: 512 EPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWLAWKGDFALKTLYYAEFNNEGPG 571
P+KS K+YLGRPWKE+SRTI+M++TI+D+I P GWL W GDFALKTLYYAE N GPG
Sbjct: 469 IPMKSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPG 528
Query: 572 AKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-WIQSTSAP 603
+ RVKWPG K + ++A +T FL D WI T P
Sbjct: 529 SNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQVP 563
BLAST of CmaCh04G018870 vs. NCBI nr
Match:
gi|659121235|ref|XP_008460558.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis melo])
HSP 1 Score: 942.6 bits (2435), Expect = 3.5e-271
Identity = 484/608 (79.61%), Postives = 531/608 (87.34%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRR ERARKFR+RV I VV A+VI+L++AAAVF+QVS SSS S
Sbjct: 1 MVFQDFDQISERRRAERARKFRKRVTIAVVSASVILLVVAAAVFVQVS------SSSKST 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
T N D S N+ K S EKMI MICNSTDYK KCES LKDGL DP
Sbjct: 61 TKNGDSGSST---GNSKKPLPPETKQVSRVEKMITMICNSTDYKGKCESTLKDGLHTDPN 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
SSDPK+LIKLAI+AAA EVKSA+KKA+ F TPEEKGAFEDCKVL+EDAVEELEMSM+
Sbjct: 121 SSDPKDLIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAVEELEMSMSE 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
VSKKN GKLT K TPDLNNWLSAVMSYHETCVDGFP+G KMK+EMEK+ K+GKELTSNS+
Sbjct: 181 VSKKNMGKLTAKTTPDLNNWLSAVMSYHETCVDGFPEG-KMKSEMEKVVKAGKELTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVV 300
AMISQVA+FFS FE EGAA+RRRLM +P+WM+SNERRMLKGA AG+KP PNVV
Sbjct: 241 AMISQVASFFSTFEMP---EGAASRRRLMATKGVPTWMNSNERRMLKGAAAGEKPKPNVV 300
Query: 301 VAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSM 360
VA+DGSG FKTINEALAAMPAKYDGRYVIY+KEGIY+E VVITKKMVNVTMYGDGSQKSM
Sbjct: 301 VAKDGSGEFKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSM 360
Query: 361 ISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCR 420
ISG+KNF DGVRTFQTA+FVALG+GF QAIGFRN AGPEKHQAVAARVQADRAIF+NCR
Sbjct: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCR 420
Query: 421 FDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGR 480
F+GYQDTLYAQAHR FYRSCLI GTIDFIFGDAAAIFQNCNMM++KPLDNQQNIVTAQGR
Sbjct: 421 FEGYQDTLYAQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNIVTAQGR 480
Query: 481 SDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWL 540
+D+HETT IVLQ CKIL DKTLE VKSQFKSYLGRPWKE+SRTIVMES IED+IHPDGW+
Sbjct: 481 TDKHETTGIVLQNCKILPDKTLEAVKSQFKSYLGRPWKEFSRTIVMESKIEDVIHPDGWM 540
Query: 541 AWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTS 600
AW+GDFALKTLYYAEFNN+GPGAKT+ RVKWPG+KVIDK+EAAKFTIG+FLE+DWI+STS
Sbjct: 541 AWQGDFALKTLYYAEFNNQGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTS 595
Query: 601 APVHVGLF 609
APVHVGLF
Sbjct: 601 APVHVGLF 595
BLAST of CmaCh04G018870 vs. NCBI nr
Match:
gi|449449216|ref|XP_004142361.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Cucumis sativus])
HSP 1 Score: 940.3 bits (2429), Expect = 1.7e-270
Identity = 474/608 (77.96%), Postives = 537/608 (88.32%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRR ERARKFR+RVII VV A+VI+L++AAAVF+QVS + S++ + S
Sbjct: 1 MVFQDFDQISERRRAERARKFRKRVIIAVVSASVILLVVAAAVFVQVSSSKSTTKNDDSG 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
+S+ + K P++ K S EKMI MICNSTDYK KCES LKDG+ DP
Sbjct: 61 SSSGNSKQPLPPET----------KQVSRVEKMITMICNSTDYKGKCESTLKDGVHTDPN 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
SSDPK+LIKLAI+AAA EVKSA+KKA+ F TPEEKGAFEDCKVL+EDA+EELEMSM+
Sbjct: 121 SSDPKDLIKLAISAAAHEVKSAVKKASGFNFATPEEKGAFEDCKVLLEDAIEELEMSMSE 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
V+KKN GKLT K TP+LNNWLSAVMSYHETCVDGFP+G KMK+++EK+ K+GKELTSNS+
Sbjct: 181 VNKKNMGKLTAKTTPNLNNWLSAVMSYHETCVDGFPEG-KMKSDIEKVVKAGKELTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVV 300
AMISQVA+FFS FE EGAA+RRRLM + +P+WMD N+RRMLKGA AG+KP PNVV
Sbjct: 241 AMISQVASFFSTFEMP---EGAASRRRLMTTNGVPTWMDRNQRRMLKGAAAGEKPKPNVV 300
Query: 301 VAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSM 360
VA+DGSG FKTINEALAAMPAKYDGRYVIY+KEGIY+E VVITKKMVNVTMYGDGSQKSM
Sbjct: 301 VAKDGSGEFKTINEALAAMPAKYDGRYVIYVKEGIYDETVVITKKMVNVTMYGDGSQKSM 360
Query: 361 ISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCR 420
ISG+KNF DGVRTFQTA+FVALG+GF QAIGFRN AGPEKHQAVAARVQADRAIF+NCR
Sbjct: 361 ISGSKNFVDGVRTFQTATFVALGEGFLGQAIGFRNIAGPEKHQAVAARVQADRAIFVNCR 420
Query: 421 FDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGR 480
F+GYQDTLY QAHR FYRSCLI GTIDFIFGDAAAIFQNCNMM++KPLDNQQN+VTAQGR
Sbjct: 421 FEGYQDTLYTQAHRQFYRSCLITGTIDFIFGDAAAIFQNCNMMIRKPLDNQQNMVTAQGR 480
Query: 481 SDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWL 540
+D+HETT IVLQ CKIL DKTLEPVKSQFKSYLGRPWKE+SRTIVMESTIED+IHPDGW+
Sbjct: 481 TDKHETTGIVLQNCKILPDKTLEPVKSQFKSYLGRPWKEFSRTIVMESTIEDVIHPDGWM 540
Query: 541 AWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTS 600
AW+GDFALKTLYYAEFNN+GPGAKT+ RVKWPG+KVIDK+EAAKFTIG+FLE+DWI+STS
Sbjct: 541 AWEGDFALKTLYYAEFNNKGPGAKTDARVKWPGYKVIDKDEAAKFTIGTFLELDWIESTS 594
Query: 601 APVHVGLF 609
APVHVGLF
Sbjct: 601 APVHVGLF 594
BLAST of CmaCh04G018870 vs. NCBI nr
Match:
gi|802628667|ref|XP_012077133.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Jatropha curcas])
HSP 1 Score: 730.7 bits (1885), Expect = 2.1e-207
Identity = 377/611 (61.70%), Postives = 469/611 (76.76%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFDQISERRRLER +KFR+R+ I + +++++I VF +S + ++ SK
Sbjct: 1 MVFQDFDQISERRRLEREQKFRKRITIAAITIVLLLVIIGCGVFAIIS---ENKNTKRSK 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
S+++ K +AKD + +++I ICN TDYK+ C S L+ +K DP
Sbjct: 61 ESSQESKG--------------SAKDVAQVDRVIRRICNGTDYKDTCVSSLESEVKKDPS 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
S++PK +++LAI A E+ LKKA++ KF +P+EK AFEDCKVL++DA EEL+ S+T
Sbjct: 121 SANPKAILRLAIEATDDEMGVVLKKASSFKFESPQEKAAFEDCKVLIKDAKEELKESLTH 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
+ KL E P+LNN LSAVMSY +TC+DGFPDG K+K++MEK + KELTSNS+
Sbjct: 181 AGVDIK-KLAENE-PELNNLLSAVMSYQQTCIDGFPDG-KLKSDMEKTFNASKELTSNSL 240
Query: 241 AMISQVATFFSKF-ESEGPGEGAATRRRL--MDQDELPSWMDSNERRMLKGATAGDKPTP 300
AM+S + +F S F E AT+ L MD+DELPSW+ + +RR+LK A DKPTP
Sbjct: 241 AMVSSLTSFLSSFGEINHSRRLLATKPNLSSMDKDELPSWISNEDRRILK-ADDSDKPTP 300
Query: 301 NVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQ 360
NV+VA+DGSG FKTI+EAL AMP KY GRY+IY+K G+Y+E V + KKM N+TMYGDGSQ
Sbjct: 301 NVIVAKDGSGKFKTISEALKAMPKKYKGRYIIYVKAGVYDEVVTVEKKMENITMYGDGSQ 360
Query: 361 KSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFL 420
KS+I+GNKNF DGV+TF+TASFVALG+GF A+AIGFRNTAGPEKHQAVAARVQADR IFL
Sbjct: 361 KSIITGNKNFKDGVQTFRTASFVALGNGFMAKAIGFRNTAGPEKHQAVAARVQADRTIFL 420
Query: 421 NCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTA 480
NCRF+GYQDTLY Q HR +YRSC+IAGTIDFIFGDAAAI QNC ++++KPL+NQQNIVTA
Sbjct: 421 NCRFEGYQDTLYVQTHRQYYRSCVIAGTIDFIFGDAAAILQNCQIVIRKPLENQQNIVTA 480
Query: 481 QGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPD 540
QGR D HETT IVLQ C+I DK+L P K++FKSYLGRPWKEYSRTIVMESTIED+IHPD
Sbjct: 481 QGRIDPHETTGIVLQNCRIQPDKSLVPDKAKFKSYLGRPWKEYSRTIVMESTIEDLIHPD 540
Query: 541 GWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQ 600
GWLAW+G+ LKTLYYAEFNN+GPGA T+ RVKWPG+ VIDKEEA K+TI F++ DWI
Sbjct: 541 GWLAWEGEKGLKTLYYAEFNNKGPGASTKARVKWPGYHVIDKEEANKYTIEPFIQGDWIN 590
Query: 601 STSAPVHVGLF 609
APVH GLF
Sbjct: 601 DMGAPVHFGLF 590
BLAST of CmaCh04G018870 vs. NCBI nr
Match:
gi|1009142836|ref|XP_015888938.1| (PREDICTED: putative pectinesterase/pectinesterase inhibitor 45 [Ziziphus jujuba])
HSP 1 Score: 727.2 bits (1876), Expect = 2.3e-206
Identity = 363/608 (59.70%), Postives = 455/608 (74.84%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
MVFQDFD+ISERRR ER RKF++RV I ++ V++++IAA V + V+
Sbjct: 1 MVFQDFDKISERRRAERERKFKKRVTIGLITGLVLLVIIAAGVIVLVT------------ 60
Query: 61 TSNKDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADPK 120
T+N + S + K+++ T +D EK+I +C+STDYK++C L K P+
Sbjct: 61 TNNNNNDSNSNNKNSSTTSKSNKGRDVVKVEKVITSVCSSTDYKQRCHDSLTAAAKKQPE 120
Query: 121 SSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMTA 180
S PK+ IK+AI+AA+ E+ A K +N F TPEEK AF DCKVLMEDA+EELE S
Sbjct: 121 LSQPKDFIKVAISAASDELTKAFNKTSNFTFNTPEEKAAFGDCKVLMEDALEELEASGKH 180
Query: 181 VSKKNQGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSNSM 240
V TPDLN+WLSAV SY +TC+DGFP G K+K +++ + KE TSNS+
Sbjct: 181 VGDNATLTALSSKTPDLNSWLSAVQSYQQTCIDGFPVG-KLKTDVKNAFNATKEFTSNSL 240
Query: 241 AMISQVATFFSKFESEGPGEGAATRRRLMDQDELPSWMDSNERRMLKGATAGDKPTPNVV 300
A+IS+VA+F S ++ G G AT R L+D D P+WM +RRMLK + K TPNV
Sbjct: 241 ALISEVASFLSTLQTPG-SPGPATTRHLLDTDGFPTWMSHEDRRMLKASDV--KLTPNVT 300
Query: 301 VAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGSQKSM 360
VA+DGSG FKTIN ALAAMPA Y GR+VIY+KEG+Y+E V++TKKMVNVT+YGDGSQKS+
Sbjct: 301 VAKDGSGKFKTINAALAAMPANYTGRFVIYVKEGVYDETVIVTKKMVNVTIYGDGSQKSI 360
Query: 361 ISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIFLNCR 420
I+G KNF DGVRTF+TASF ALG+GF +A+GFRNTAGPEKHQAVAAR+Q+DRAIF+NCR
Sbjct: 361 ITGKKNFVDGVRTFETASFAALGEGFMGKAMGFRNTAGPEKHQAVAARIQSDRAIFVNCR 420
Query: 421 FDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVTAQGR 480
F+GYQDTLYAQ HR FYRSC+IAGT+DFIFGDAAAIFQNCN++V+KPLDNQQNIVTAQGR
Sbjct: 421 FEGYQDTLYAQTHRQFYRSCVIAGTVDFIFGDAAAIFQNCNLVVRKPLDNQQNIVTAQGR 480
Query: 481 SDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHPDGWL 540
+D ETT VLQ C+++ADK L PVK + +SYLGRPWKE+SRTI MES IEDIIHPDGW+
Sbjct: 481 TDVRETTGFVLQNCRVMADKALVPVKEKIRSYLGRPWKEFSRTIFMESDIEDIIHPDGWM 540
Query: 541 AWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVDWIQSTS 600
W+G+FALKTLYYAE+NN+G G+KT RVKWPG+KVIDK+EA KFT+ +FL W+ +T
Sbjct: 541 PWEGEFALKTLYYAEYNNKGAGSKTTARVKWPGYKVIDKQEANKFTVENFLPGSWLNATG 592
Query: 601 APVHVGLF 609
PV +
Sbjct: 601 VPVQKSFY 592
BLAST of CmaCh04G018870 vs. NCBI nr
Match:
gi|645274006|ref|XP_008242146.1| (PREDICTED: pectinesterase-like [Prunus mume])
HSP 1 Score: 711.8 bits (1836), Expect = 1.0e-201
Identity = 370/612 (60.46%), Postives = 467/612 (76.31%), Query Frame = 1
Query: 1 MVFQDFDQISERRRLERARKFRQRVIITVVLATVIILLIAAAVFIQVSPAPSSSSSSSSK 60
M FQDFDQISERR+ ERARK R+R+II VV+ ++IL+ A + ++ K
Sbjct: 1 MAFQDFDQISERRKAERARKMRKRIIIAVVVVVLLILIAVGAYLLLNKLNSKKGNAKQDK 60
Query: 61 TSN-KDGKSKKHPKSNTDTDTGTNAKDASSAEKMIVMICNSTDYKEKCESILKDGLKADP 120
T+N K +K PK++ K + EK++ +C +TDYK+KCESI+ A
Sbjct: 61 TANSKPAPAKAQPKNDQ-----AKKKVPAKGEKIMKQMCGATDYKDKCESIIGKAKDA-- 120
Query: 121 KSSDPKELIKLAITAAAAEVKSALKKATNLKFTTPEEKGAFEDCKVLMEDAVEELEMSMT 180
S PKE IK AI+ A+ E + A K++ L F +PEEKGAFEDCKVL EDA++EL +++
Sbjct: 121 --SKPKEFIKTAISVASDEARIAYSKSSELTFNSPEEKGAFEDCKVLFEDAMDELGDAIS 180
Query: 181 AVSKKN-QGKLTEKATPDLNNWLSAVMSYHETCVDGFPDGSKMKAEMEKIGKSGKELTSN 240
+ GK+ T LN WLSAV+SY +TCVDGFPDG K+K+++EK+ ++ KE TSN
Sbjct: 181 QIGNTTASGKI---RTGLLNTWLSAVISYQQTCVDGFPDG-KLKSDLEKMLQASKEFTSN 240
Query: 241 SMAMISQVATFFSKFESEGPGEGAATRRRLMDQ--DELPSWMDSNERRMLKGATAGDKPT 300
S+AM+S S+F+ + +RRL+ Q D P+WM ERR+LK +KPT
Sbjct: 241 SLAMLS----LLSQFQLPVTAAVSGAKRRLLAQNKDRFPTWMSHEERRVLK--KNDEKPT 300
Query: 301 PNVVVAQDGSGNFKTINEALAAMPAKYDGRYVIYLKEGIYEEAVVITKKMVNVTMYGDGS 360
PNV VA+DGSGNFKTI+EALAAMPAKY+GRY+IY+K G+Y+E V++TKKM NVT+YGDGS
Sbjct: 301 PNVTVAKDGSGNFKTISEALAAMPAKYEGRYIIYVKGGVYDETVIVTKKMPNVTIYGDGS 360
Query: 361 QKSMISGNKNFADGVRTFQTASFVALGDGFFAQAIGFRNTAGPEKHQAVAARVQADRAIF 420
QKS+I+GNKNFADGVRTFQTASF ALG+GF A+++GFRNTAGPEKHQAVAARVQADRAIF
Sbjct: 361 QKSIITGNKNFADGVRTFQTASFAALGEGFMAKSMGFRNTAGPEKHQAVAARVQADRAIF 420
Query: 421 LNCRFDGYQDTLYAQAHRHFYRSCLIAGTIDFIFGDAAAIFQNCNMMVKKPLDNQQNIVT 480
LNCRF+GYQDTLYAQ HR FY+SC+I+GT+DFIFGDAAAIFQNC + V+KP++NQQNIVT
Sbjct: 421 LNCRFEGYQDTLYAQTHRQFYKSCVISGTVDFIFGDAAAIFQNCLIYVRKPMENQQNIVT 480
Query: 481 AQGRSDRHETTAIVLQRCKILADKTLEPVKSQFKSYLGRPWKEYSRTIVMESTIEDIIHP 540
AQGR+D+ ETT IVLQ CKI+ DK LEPVKSQFK+YLGRPWKE+SRTIVM+STIED+IHP
Sbjct: 481 AQGRTDKQETTGIVLQNCKIMPDKDLEPVKSQFKTYLGRPWKEFSRTIVMDSTIEDLIHP 540
Query: 541 DGWLAWKGDFALKTLYYAEFNNEGPGAKTEGRVKWPGHKVIDKEEAAKFTIGSFLEVD-W 600
DGW W+GDFALKTLYYAE+NN+GPGAKT+ RVKW G+KVIDK+EA K+T+G FL+ + W
Sbjct: 541 DGWTPWQGDFALKTLYYAEYNNKGPGAKTDNRVKWSGYKVIDKQEAMKYTVGPFLKGNAW 593
Query: 601 IQSTSAPVHVGL 608
++ PV GL
Sbjct: 601 LRVKGVPVRFGL 593
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PME45_ARATH | 6.1e-167 | 50.82 | Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana GN=P... | [more] |
| PME13_ARATH | 1.8e-158 | 48.56 | Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana GN=P... | [more] |
| PME58_ARATH | 1.2e-127 | 45.47 | Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana GN=P... | [more] |
| PME28_ARATH | 1.6e-122 | 41.21 | Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana GN=P... | [more] |
| PME23_ARATH | 2.3e-121 | 45.68 | Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana GN=P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KX33_CUCSA | 1.2e-270 | 77.96 | Pectinesterase OS=Cucumis sativus GN=Csa_5G630740 PE=4 SV=1 | [more] |
| A0A067KCM7_JATCU | 1.4e-207 | 61.70 | Pectinesterase OS=Jatropha curcas GN=JCGZ_07552 PE=4 SV=1 | [more] |
| M5VTB0_PRUPE | 1.2e-201 | 60.46 | Pectinesterase OS=Prunus persica GN=PRUPE_ppa023802mg PE=4 SV=1 | [more] |
| B9RR24_RICCO | 1.5e-201 | 59.41 | Pectinesterase OS=Ricinus communis GN=RCOM_0708480 PE=4 SV=1 | [more] |
| U5G7D7_POPTR | 2.9e-200 | 58.37 | Pectinesterase OS=Populus trichocarpa GN=POPTR_0006s13690g PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT4G33230.1 | 3.4e-168 | 50.82 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT2G26450.1 | 1.0e-159 | 48.56 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT5G49180.1 | 7.0e-129 | 45.47 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT5G27870.1 | 8.9e-124 | 41.21 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
| AT3G06830.1 | 1.3e-122 | 45.68 | Plant invertase/pectin methylesterase inhibitor superfamily | [more] |
