CmaCh04G014560 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G014560
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	3-oxo-5-alpha-steroid 4-dehydrogenase family protein
Location	Cma_Chr04 : 7411513 .. 7411953 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCTGAGCGATGCGATTATTGCCTCGATTACCTATATACTCCTTACCGGCAGCACGATCTACTCTCAGATGGTTGCGCAGGATCTGGCGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTATCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTAGGAATCGCTTTCGTTTCGCAGACACTGTTCGCAATGGTCTTCGCCATCGGATCCGCTCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCCATCCTGCCTTTCGTTTTTTGA

mRNA sequence

ATGGGCCTGAGCGATGCGATTATTGCCTCGATTACCTATATACTCCTTACCGGCAGCACGATCTACTCTCAGATGGTTGCGCAGGATCTGGCGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTATCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTAGGAATCGCTTTCGTTTCGCAGACACTGTTCGCAATGGTCTTCGCCATCGGATCCGCTCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCCATCCTGCCTTTCGTTTTTTGA

Coding sequence (CDS)

ATGGGCCTGAGCGATGCGATTATTGCCTCGATTACCTATATACTCCTTACCGGCAGCACGATCTACTCTCAGATGGTTGCGCAGGATCTGGCGGAGCCGCAGATCGATCTGAGGGCGGCTGGATTGGCGATGTACTGGATTGGAATCACTGGAAATCTGTATCACCACGTTCTACTATCGAAATTGAGGAAAGAAGGCGATAGAGAGTACAGGATTCCAAGGGGAGGGATGTTTGAAGTGATCGTGTGCCCTCACTACCTGTTTGAGATGGTAGAGTTCTTAGGAATCGCTTTCGTTTCGCAGACACTGTTCGCAATGGTCTTCGCCATCGGATCCGCTCTGTTCTTGATTGGCCGAAGCTACGCCACCAGGAATTGGTACGTTTCTAAATTTGAAGATTTTCCTCGCCATGTTAAAGCCATCCTGCCTTTCGTTTTTTGA

Protein sequence

MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF

Homology

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BLAST of CmaCh04G014560 vs. Swiss-Prot
Match: S5A1_RAT (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-16
Identity = 41/105 (39.05%), Postives = 65/105 (61.90%), Query Frame = 1


Query: 41  GLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 100
           G A++ +G+  N++   +L  LRK G+  Y+IPRGG+FE +   +Y  E+VE+ G A  S
Sbjct: 154 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAANYFGELVEWCGFALAS 213

Query: 101 QTLFAMVFAIGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFV 146
            +L  +VFA+ +   L+ R+     WY  KFED+P+  K ++PFV
Sbjct: 214 WSLQGVVFALFTLSTLLTRAKQHHQWYHEKFEDYPKSRKILIPFV 258

[Close]

BLAST of CmaCh04G014560 vs. Swiss-Prot
Match: S5A1_MOUSE (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-16
Identity = 40/105 (38.10%), Postives = 65/105 (61.90%), Query Frame = 1


Query: 41  GLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 100
           G A++ +G+  N++   +L  LRK G+  Y+IPRGG+FE +   +Y  E+VE+ G A  S
Sbjct: 150 GFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFGELVEWCGFALAS 209

Query: 101 QTLFAMVFAIGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFV 146
            +L  +VFA+ +   L  R+     WY+ KFED+P+  K ++PF+
Sbjct: 210 WSLQGVVFALFTLCALFTRARQHHQWYLEKFEDYPKTRKILIPFL 254

[Close]

BLAST of CmaCh04G014560 vs. Swiss-Prot
Match: DET2_GOSHI (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.1e-16
Identity = 41/108 (37.96%), Postives = 72/108 (66.67%), Query Frame = 1


Query: 40  AGLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFV 99
           AGL ++ +G+  N+    +L  L+K+GD  Y+IPRGG+FE++ CP+Y  E++E+ G A +
Sbjct: 151 AGLLIFVVGMWVNVRADKVLVGLKKQGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVM 210

Query: 100 SQTLFAMVFAIGSALFLIGRSYATRNWYVSKF-EDFPRHVKAILPFVF 147
           + +     F + +   L+ R+ ATR WY+ KF +D+P+  KA++PF++
Sbjct: 211 TWSWVGFGFFLYTCANLMPRARATRLWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of CmaCh04G014560 vs. Swiss-Prot
Match: S5A1_HUMAN (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.0e-16
Identity = 40/106 (37.74%), Postives = 63/106 (59.43%), Query Frame = 1


Query: 41  GLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 100
           G  ++  G+  N++   +L  LRK GD  Y+IPRGG+FE +   +Y  E++E+ G A  S
Sbjct: 154 GFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALAS 213

Query: 101 QTLFAMVFAIGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF 147
            ++    FA  +  FL GR+     WY+ KFE++P+  K I+PF+F
Sbjct: 214 WSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of CmaCh04G014560 vs. Swiss-Prot
Match: S5A1_MACFA (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 5.3e-16
Identity = 39/106 (36.79%), Postives = 63/106 (59.43%), Query Frame = 1


Query: 41  GLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 100
           G  ++  G+  N++   +L  LRK GD  Y+IPRGG+FE +   +Y  E++E+ G A  S
Sbjct: 158 GFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALAS 217

Query: 101 QTLFAMVFAIGSALFLIGRSYATRNWYVSKFEDFPRHVKAILPFVF 147
            ++    FA  +  FL GR+     WY+ KFE++P+  K ++PF+F
Sbjct: 218 WSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of CmaCh04G014560 vs. TrEMBL
Match: A0A0A0KUP2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612860 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 8.3e-61
Identity = 113/146 (77.40%), Postives = 134/146 (91.78%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L+DAI+ S++Y+L +G+TIY+Q+VA DLAEP+IDL+AAGLAMY IGITGN YHHVLLS
Sbjct: 111 MALTDAIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLS 170

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           +LR  GDREY+IP+GG+F+VIVCPHYLFE+VEFLGIAFVSQTLF +VF +GSALFLIGRS
Sbjct: 171 RLRSGGDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRS 230

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           YATR WY+SKFEDFP H+KAILPFVF
Sbjct: 231 YATRKWYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of CmaCh04G014560 vs. TrEMBL
Match: A0A151RMQ6_CAJCA (Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034676 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 5.8e-46
Identity = 84/146 (57.53%), Postives = 117/146 (80.14%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y L T + IY+Q + Q L EP I+L   G+A++ +GI GNLYHH LLS
Sbjct: 119 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPINLLYPGIALFVVGIIGNLYHHYLLS 178

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           KLR EG++EY+IP+GG+FE+++CPHYLFE++ F G++F+SQTL+A  F +G+ L+L+GRS
Sbjct: 179 KLRGEGEKEYKIPKGGLFELVICPHYLFEIMVFYGVSFISQTLYAFSFTVGTTLYLLGRS 238

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           Y TR WY+SKFEDFP+HVKA++PFVF
Sbjct: 239 YVTRKWYLSKFEDFPKHVKAVIPFVF 264

[Close]

BLAST of CmaCh04G014560 vs. TrEMBL
Match: I1JDE1_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.3e-45
Identity = 84/146 (57.53%), Postives = 114/146 (78.08%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y L T + IY+Q + Q L EP IDL   G+ ++ +GI GN YHH LLS
Sbjct: 121 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLS 180

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
            LR +G++EY+IP+GGMFE+++CPHYLFE++EF G +F+SQTL+A  F +G+ L+L+GRS
Sbjct: 181 NLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRS 240

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           Y+TR WY+SKFEDFP HVKAI+PFVF
Sbjct: 241 YSTRKWYLSKFEDFPEHVKAIIPFVF 266

[Close]

BLAST of CmaCh04G014560 vs. TrEMBL
Match: A0A072UJC1_MEDTR (3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Medicago truncatula GN=MTR_6g037340 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 6.4e-45
Identity = 79/146 (54.11%), Postives = 120/146 (82.19%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y++ T + IY Q + +DL EPQI+L   G+ ++ +GI GN YHH LLS
Sbjct: 123 MILDSAIPITLSYLMSTITMIYGQHLTKDLPEPQINLIYPGIVLFLVGIIGNFYHHFLLS 182

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           KLR +G++EY+IP+GG+F +++CPHY+FE+++F G+ F+SQTL++++FAIG+  +L+GRS
Sbjct: 183 KLRGKGEKEYKIPKGGLFGIVICPHYMFEIIDFYGVFFISQTLYSLIFAIGTTFYLLGRS 242

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           +ATRNWY+SKFEDFP++VKA++PFVF
Sbjct: 243 HATRNWYLSKFEDFPKNVKAVIPFVF 268

[Close]

BLAST of CmaCh04G014560 vs. TrEMBL
Match: A0A151RMW0_CAJCA (Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034677 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 6.4e-45
Identity = 82/146 (56.16%), Postives = 112/146 (76.71%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L   I  S+ Y + T   IY Q + Q L EP I+L   G+ ++ +GI+GNLYHH LLS
Sbjct: 119 MALDTTITISLNYFISTSLMIYDQNLTQGLPEPPINLVYPGIVLFVVGISGNLYHHYLLS 178

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           KLR +G++EY+IPRGG FE+++CPHYLFE++ FLG++F+SQT +   F +GS ++L+GRS
Sbjct: 179 KLRGKGEKEYKIPRGGCFELVICPHYLFEIIGFLGVSFISQTFYPFSFTLGSTIYLMGRS 238

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           YATR WY+SKFEDFP+HVKA++PFVF
Sbjct: 239 YATRKWYLSKFEDFPKHVKAVIPFVF 264

[Close]

BLAST of CmaCh04G014560 vs. TAIR10
Match: AT5G16010.1 (AT5G16010.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 179.5 bits (454), Expect = 1.5e-45
Identity = 79/147 (53.74%), Postives = 112/147 (76.19%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M +  A+  S +Y   T   +YSQ +   L EP  D++ AG+ M+ +GI GNLYHHVLL+
Sbjct: 122 MAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLLA 181

Query: 61  KLRKE-GDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGR 120
           KLRKE G +EY+IP+GG+F++I+CPHYLFE++ F     +SQT+++  FA+G+ L+LIGR
Sbjct: 182 KLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIGR 241

Query: 121 SYATRNWYVSKFEDFPRHVKAILPFVF 147
           SYATR WY+SKF+DFP+H+KA++PFVF
Sbjct: 242 SYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of CmaCh04G014560 vs. TAIR10
Match: AT2G38050.1 (AT2G38050.1 3-oxo-5-alpha-steroid 4-dehydrogenase family protein)

HSP 1 Score: 75.5 bits (184), Expect = 3.1e-14
Identity = 38/107 (35.51%), Postives = 66/107 (61.68%), Query Frame = 1


Query: 41  GLAMYWIGITGNLYHHVLLSKLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVS 100
           G+ ++  G+  N+     L +L+KE    Y IPRGG FE++ CP+Y  E +E+LG A ++
Sbjct: 156 GMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMT 215

Query: 101 QTLFAMVFAIGSALFLIGRSYATRNWYVSKF-EDFPRHVKAILPFVF 147
            +   + F + +   L  R+ A+  WY++KF E++P+  KA++PFV+
Sbjct: 216 WSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of CmaCh04G014560 vs. NCBI nr
Match: gi|659091767|ref|XP_008446721.1| (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo])

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-61
Identity = 116/146 (79.45%), Postives = 133/146 (91.10%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L+DAII S+ Y+L TG+TIY+Q+V+ DL EP+IDL+AAGLA+Y IGITGN YHHVLLS
Sbjct: 116 MALTDAIITSLAYLLFTGATIYAQIVSLDLVEPEIDLKAAGLALYGIGITGNFYHHVLLS 175

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           +LR  GDREYRIPRGG+F+VIVCPHYLFE+VEFLGIAFVSQTLFA+VF +GSALFLIGRS
Sbjct: 176 RLRSGGDREYRIPRGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFAVVFVVGSALFLIGRS 235

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           YATR WY+SKFEDFP HVKAILPFVF
Sbjct: 236 YATRKWYLSKFEDFPTHVKAILPFVF 261

[Close]

BLAST of CmaCh04G014560 vs. NCBI nr
Match: gi|449435178|ref|XP_004135372.1| (PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus])

HSP 1 Score: 241.1 bits (614), Expect = 1.2e-60
Identity = 113/146 (77.40%), Postives = 134/146 (91.78%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L+DAI+ S++Y+L +G+TIY+Q+VA DLAEP+IDL+AAGLAMY IGITGN YHHVLLS
Sbjct: 111 MALTDAIMTSLSYLLFSGTTIYAQIVALDLAEPEIDLKAAGLAMYGIGITGNFYHHVLLS 170

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           +LR  GDREY+IP+GG+F+VIVCPHYLFE+VEFLGIAFVSQTLF +VF +GSALFLIGRS
Sbjct: 171 RLRSGGDREYKIPKGGLFKVIVCPHYLFEIVEFLGIAFVSQTLFVVVFVVGSALFLIGRS 230

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           YATR WY+SKFEDFP H+KAILPFVF
Sbjct: 231 YATRKWYLSKFEDFPSHIKAILPFVF 256

[Close]

BLAST of CmaCh04G014560 vs. NCBI nr
Match: gi|1012332425|gb|KYP43857.1| (Trans-2,3-enoyl-CoA reductase [Cajanus cajan])

HSP 1 Score: 191.8 bits (486), Expect = 8.3e-46
Identity = 84/146 (57.53%), Postives = 117/146 (80.14%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y L T + IY+Q + Q L EP I+L   G+A++ +GI GNLYHH LLS
Sbjct: 119 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPINLLYPGIALFVVGIIGNLYHHYLLS 178

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           KLR EG++EY+IP+GG+FE+++CPHYLFE++ F G++F+SQTL+A  F +G+ L+L+GRS
Sbjct: 179 KLRGEGEKEYKIPKGGLFELVICPHYLFEIMVFYGVSFISQTLYAFSFTVGTTLYLLGRS 238

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           Y TR WY+SKFEDFP+HVKA++PFVF
Sbjct: 239 YVTRKWYLSKFEDFPKHVKAVIPFVF 264

[Close]

BLAST of CmaCh04G014560 vs. NCBI nr
Match: gi|571439230|ref|XP_006574798.1| (PREDICTED: very-long-chain enoyl-CoA reductase-like [Glycine max])

HSP 1 Score: 190.7 bits (483), Expect = 1.9e-45
Identity = 84/146 (57.53%), Postives = 114/146 (78.08%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y L T + IY+Q + Q L EP IDL   G+ ++ +GI GN YHH LLS
Sbjct: 121 MLLDSAIPITLSYFLSTATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLS 180

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
            LR +G++EY+IP+GGMFE+++CPHYLFE++EF G +F+SQTL+A  F +G+ L+L+GRS
Sbjct: 181 NLRGKGEKEYKIPKGGMFELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLGRS 240

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           Y+TR WY+SKFEDFP HVKAI+PFVF
Sbjct: 241 YSTRKWYLSKFEDFPEHVKAIIPFVF 266

[Close]

BLAST of CmaCh04G014560 vs. NCBI nr
Match: gi|922352101|ref|XP_013451867.1| (3-oxo-5-alpha-steroid 4-dehydrogenase family protein [Medicago truncatula])

HSP 1 Score: 188.3 bits (477), Expect = 9.2e-45
Identity = 79/146 (54.11%), Postives = 120/146 (82.19%), Query Frame = 1


Query: 1   MGLSDAIIASITYILLTGSTIYSQMVAQDLAEPQIDLRAAGLAMYWIGITGNLYHHVLLS 60
           M L  AI  +++Y++ T + IY Q + +DL EPQI+L   G+ ++ +GI GN YHH LLS
Sbjct: 123 MILDSAIPITLSYLMSTITMIYGQHLTKDLPEPQINLIYPGIVLFLVGIIGNFYHHFLLS 182

Query: 61  KLRKEGDREYRIPRGGMFEVIVCPHYLFEMVEFLGIAFVSQTLFAMVFAIGSALFLIGRS 120
           KLR +G++EY+IP+GG+F +++CPHY+FE+++F G+ F+SQTL++++FAIG+  +L+GRS
Sbjct: 183 KLRGKGEKEYKIPKGGLFGIVICPHYMFEIIDFYGVFFISQTLYSLIFAIGTTFYLLGRS 242

Query: 121 YATRNWYVSKFEDFPRHVKAILPFVF 147
           +ATRNWY+SKFEDFP++VKA++PFVF
Sbjct: 243 HATRNWYLSKFEDFPKNVKAVIPFVF 268

The following BLAST results are available for this feature:

BLAST of CmaCh04G014560 vs. Swiss-Prot


Match Name	E-value	Identity	Description
S5A1_RAT	1.4e-16	39.05	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus GN=Srd5a1 PE=1 SV=2	[more]
S5A1_MOUSE	2.4e-16	38.10	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus GN=Srd5a1 PE=2 SV=2	[more]
DET2_GOSHI	3.1e-16	37.96	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum GN=DET2 PE=1 SV=1	[more]
S5A1_HUMAN	4.0e-16	37.74	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens GN=SRD5A1 PE=1 SV=1	[more]
S5A1_MACFA	5.3e-16	36.79	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis GN=SRD5A1 PE=1 SV...	[more]

BLAST of CmaCh04G014560 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KUP2_CUCSA	8.3e-61	77.40	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G612860 PE=4 SV=1	[more]
A0A151RMQ6_CAJCA	5.8e-46	57.53	Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034676 PE=4 SV=1	[more]
I1JDE1_SOYBN	1.3e-45	57.53	Uncharacterized protein OS=Glycine max GN=GLYMA_02G081200 PE=4 SV=1	[more]
A0A072UJC1_MEDTR	6.4e-45	54.11	3-oxo-5-alpha-steroid 4-dehydrogenase family protein OS=Medicago truncatula GN=M...	[more]
A0A151RMW0_CAJCA	6.4e-45	56.16	Trans-2,3-enoyl-CoA reductase OS=Cajanus cajan GN=KK1_034677 PE=4 SV=1	[more]

BLAST of CmaCh04G014560 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G16010.1	1.5e-45	53.74	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	3.1e-14	35.51	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

BLAST of CmaCh04G014560 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659091767\|ref\|XP_008446721.1\|	1.8e-61	79.45	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis melo]	[more]
gi\|449435178\|ref\|XP_004135372.1\|	1.2e-60	77.40	PREDICTED: steroid 5-alpha-reductase DET2-like [Cucumis sativus]	[more]
gi\|1012332425\|gb\|KYP43857.1\|	8.3e-46	57.53	Trans-2,3-enoyl-CoA reductase [Cajanus cajan]	[more]
gi\|571439230\|ref\|XP_006574798.1\|	1.9e-45	57.53	PREDICTED: very-long-chain enoyl-CoA reductase-like [Glycine max]	[more]
gi\|922352101\|ref\|XP_013451867.1\|	9.2e-45	54.11	3-oxo-5-alpha-steroid 4-dehydrogenase family protein [Medicago truncatula]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001104	3-oxo-5_a-steroid_4-DH_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Vocabulary: **Cellular Component**
Term	Definition
GO:0005737	cytoplasm
GO:0016021	integral component of membrane

Vocabulary: **Biological Process**
Term	Definition
GO:0006629	lipid metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G014560.1	CmaCh04G014560.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 9..146 score: 2.6
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROFILE	PS50244	S5A_REDUCTASE	coord: 41..110 score: 2
None	No IPR available	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 1..146 score: 8.5
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 1..146 score: 8.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G014560	Cla020467	Watermelon (97103) v1	cmawmB699

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G014560	Cucurbita pepo (Zucchini)	cmacpeB700
CmaCh04G014560	Cucurbita pepo (Zucchini)	cmacpeB720
CmaCh04G014560	Bottle gourd (USVL1VR-Ls)	cmalsiB669
CmaCh04G014560	Cucumber (Gy14) v2	cgybcmaB672
CmaCh04G014560	Melon (DHL92) v3.6.1	cmamedB726
CmaCh04G014560	Silver-seed gourd	carcmaB1170
CmaCh04G014560	Silver-seed gourd	carcmaB1457
CmaCh04G014560	Cucumber (Chinese Long) v3	cmacucB0885
CmaCh04G014560	Watermelon (97103) v2	cmawmbB758
CmaCh04G014560	Wax gourd	cmawgoB0897
CmaCh04G014560	Cucurbita maxima (Rimu)	cmacmaB402
CmaCh04G014560	Cucumber (Gy14) v1	cgycmaB0801
CmaCh04G014560	Cucurbita moschata (Rifu)	cmacmoB706
CmaCh04G014560	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G014560	Wild cucumber (PI 183967)	cmacpiB755
CmaCh04G014560	Cucumber (Chinese Long) v2	cmacuB748
CmaCh04G014560	Melon (DHL92) v3.5.1	cmameB640
CmaCh04G014560	Watermelon (Charleston Gray)	cmawcgB655
CmaCh04G014560	Watermelon (97103) v1	cmawmB667