CmaCh04G014410 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G014410
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Subtilisin-like serine endopeptidase family protein
Location	Cma_Chr04 : 7342966 .. 7343496 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCATAAACGTAACCCAGACGGCGAGTTAGCATACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTTAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAATACTTCTTGTTCAGCCAACAATTCCGGCACAGTTTTGGAACTCAACTACCCATCCTTTGCGCTTTCCACAAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTCGGGTCGATCTCTGCGACGTCGTATAAAGCCGCCATAGTTAACCCATGGAGAATTCTTAAAATTACGGTGAAGCCCTCTGTTCTTTGGTTCAAGAGGTTGGGAGAGGAGCTAAGCTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTGTTTAATGCTAATCTTCACTCTTAG

mRNA sequence

ATGAGCCATAAACGTAACCCAGACGGCGAGTTAGCATACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTTAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAATACTTCTTGTTCAGCCAACAATTCCGGCACAGTTTTGGAACTCAACTACCCATCCTTTGCGCTTTCCACAAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTCGGGTCGATCTCTGCGACGTCGTATAAAGCCGCCATAGTTAACCCATGGAGAATTCTTAAAATTACGGTGAAGCCCTCTGTTCTTTGGTTCAAGAGGTTGGGAGAGGAGCTAAGCTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTGTTTAATGCTAATCTTCACTCTTAG

Coding sequence (CDS)

ATGAGCCATAAACGTAACCCAGACGGCGAGTTAGCATACGGCGCGGGCCACATAAACCCACTGGGCGCAGTCCATCCAGGCTTAATCTACAACGCTTTAGAGACCGACTACGTGAAGTTTCTATGCGGTCAAGGCTACTCCACGGAGTCGCTCCGGCGACTCTCCGACGACAATACTTCTTGTTCAGCCAACAATTCCGGCACAGTTTTGGAACTCAACTACCCATCCTTTGCGCTTTCCACAAACGCCTCAAACCCCATCAGCCAAGTTTTCAGAAGAAGGGTCACAAATGTCGGGTCGATCTCTGCGACGTCGTATAAAGCCGCCATAGTTAACCCATGGAGAATTCTTAAAATTACGGTGAAGCCCTCTGTTCTTTGGTTCAAGAGGTTGGGAGAGGAGCTAAGCTTTGAGGTTAGAATTGGAGGAAAAATTAGGAAAGGTATTGAATCGGCGGCTTTGGTGTGGGATGATGGTAAGCATAAAGTGAGGAGTCCTATAACCGTGTTTAATGCTAATCTTCACTCTTAG

Protein sequence

MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANLHS

Homology

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BLAST of CmaCh04G014410 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 5.2e-42
Identity = 85/169 (50.30%), Postives = 121/169 (71.60%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           M+ + NP  E AYG+GH+NPL AV PGL+Y+A E+DYVKFLCGQGY+T+++RR++ D ++
Sbjct: 561 MNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSA 620

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           C++ N+G V +LNYPSF LS + S   +Q F R +T+V    A++Y+A I  P + L I+
Sbjct: 621 CTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTSVAP-QASTYRAMISAP-QGLTIS 680

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITV 170
           V P+VL F  LG+  SF + + G I+  + SA+LVW DG H VRSPIT+
Sbjct: 681 VNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727

[Close]

BLAST of CmaCh04G014410 vs. Swiss-Prot
Match: SBT45_ARATH (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.2e-32
Identity = 77/171 (45.03%), Postives = 107/171 (62.57%), Query Frame = 1


Query: 6   NPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANN 65
           N   E AYGAGH++P+ A+HPGL+Y A ++D++ FLCG  Y+ ++LR +S D++SC+   
Sbjct: 571 NELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQ 630

Query: 66  SGTV-LELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPS 125
           + ++   LNYPS     +A+ P   +FRR VTNVG  +AT YKA +V     LK+ V P+
Sbjct: 631 TKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPA 690

Query: 126 VLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVFNAN 174
           VL  K L E+ SF V   G   K   + SA L+W DG H VRSPI V+  N
Sbjct: 691 VLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738

[Close]

BLAST of CmaCh04G014410 vs. Swiss-Prot
Match: SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.4e-31
Identity = 75/168 (44.64%), Postives = 105/168 (62.50%), Query Frame = 1


Query: 6   NPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANN 65
           N   E AYGAGH++P+ A+HPGL+Y A ++D++ FLCG  Y+ + LR +S D++SC+   
Sbjct: 571 NELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQ 630

Query: 66  SGTVL-ELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPS 125
           + ++   LNYPS +   + + P    FRR VTNVG  +AT YKA +V     LK+ V P+
Sbjct: 631 TKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPA 690

Query: 126 VLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVF 171
           VL  K L E+ SF V + G   K   + SA L+W DG H VRSPI V+
Sbjct: 691 VLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

[Close]

BLAST of CmaCh04G014410 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.0e-29
Identity = 71/173 (41.04%), Postives = 104/173 (60.12%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           M+ K+NP+ E AYG+G INP  A  PGL+Y     DY+K LC +G+ + +L   S  N +
Sbjct: 557 MNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVT 616

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS      V +LNYP+     ++ +P +  F+R VTNVG  ++T YKA++V     L+I+
Sbjct: 617 CSERTE--VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNST-YKASVVPLQPELQIS 676

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVFN 172
           ++P +L F  L E+ SF V I GK  K     S+++VW DG H VRSPI  ++
Sbjct: 677 IEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 726

[Close]

BLAST of CmaCh04G014410 vs. Swiss-Prot
Match: SBT42_ARATH (Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana GN=SBT4.2 PE=3 SV=3)

HSP 1 Score: 130.2 bits (326), Expect = 2.2e-29
Identity = 73/174 (41.95%), Postives = 101/174 (58.05%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           M+  +N + E AYG+G +NP  AV PGL+Y   + DY+  LC   YS++ +  ++    +
Sbjct: 554 MNASKNAEAEFAYGSGFVNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFT 613

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIV-NPWRILKI 120
           CS  +  T+  LNYPS +   +AS+     F R VTNVG   +T YKA +  NP   L I
Sbjct: 614 CSEQSKLTMRNLNYPSMSAKVSASSSSDITFSRTVTNVGEKGST-YKAKLSGNP--KLSI 673

Query: 121 TVKPSVLWFKRLGEELSFEVRIGGKIRKGIE---SAALVWDDGKHKVRSPITVF 171
            V+P+ L FK  GE+ SF V + GK   GI    SA+L+W DG H VRSPI V+
Sbjct: 674 KVEPATLSFKAPGEKKSFTVTVSGKSLAGISNIVSASLIWSDGSHNVRSPIVVY 724

[Close]

BLAST of CmaCh04G014410 vs. TrEMBL
Match: A0A0A0KWL9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 5.2e-65
Identity = 126/174 (72.41%), Postives = 145/174 (83.33%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           MS K NPD E  YGAGHINPLGAVHPGLIY+A E DYV+FLCGQGY+TE L+ +S+DN +
Sbjct: 567 MSPKHNPDKEFGYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNT 626

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS+NNS TV +LNYPSFALSTN S PI+QV++R VTNVGS  AT YKA ++NPW+ L+I 
Sbjct: 627 CSSNNSDTVFDLNYPSFALSTNISKPINQVYKRTVTNVGSKYAT-YKATVINPWKNLEIK 686

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANL 175
           V PSVL FK LGE+ SFEV I GKIRK IESA+LVWDDGKHKVRSPITVF AN+
Sbjct: 687 VNPSVLSFKNLGEKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIANI 739

[Close]

BLAST of CmaCh04G014410 vs. TrEMBL
Match: A0A0A0KRC4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.0e-61
Identity = 121/174 (69.54%), Postives = 143/174 (82.18%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           MS KRN D E AYGAGH+NPLGAVHPGLIY+A E DYV+FLCGQGY+TE L+ +SD + +
Sbjct: 567 MSPKRNQDKEFAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNT 626

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS+N+S TV +LNYPSFALSTN S PI+QV+RR VTN+GS SA  YKA I+NPW+ L I 
Sbjct: 627 CSSNDSDTVFDLNYPSFALSTNISVPINQVYRRTVTNIGSRSA-MYKATIINPWKNLDIK 686

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANL 175
           V PSVL F  LGE+ SFEV I GKIR+ IESA+LVW+DGKHKVRSPITVF+A +
Sbjct: 687 VNPSVLSFTSLGEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVFDATI 739

[Close]

BLAST of CmaCh04G014410 vs. TrEMBL
Match: A0A0A0KJN5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 5.2e-49
Identity = 104/167 (62.28%), Postives = 127/167 (76.05%), Query Frame = 1


Query: 7   PDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANNS 66
           P+ E AYG+GHINPLGAV+PGLIYNA ETDY++FLC +GY+T  LR ++ DN++CS   S
Sbjct: 575 PEAEFAYGSGHINPLGAVNPGLIYNASETDYIRFLCDEGYNTTFLRIITKDNSTCSTTQS 634

Query: 67  GTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPSVL 126
             V +LNYPSFAL T+ S P SQ  +RRVTNVGS ++T YKA I  P   L ITV PS+L
Sbjct: 635 IRVYDLNYPSFALFTHISTPFSQTSKRRVTNVGSTNST-YKATISAP-SGLNITVNPSIL 694

Query: 127 WFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNAN 174
            FK L EEL+FEV   GKI + IESA+LVWDDG HKVRSPI VF+++
Sbjct: 695 SFKALEEELNFEVTFEGKIDRSIESASLVWDDGVHKVRSPIIVFDSD 739

[Close]

BLAST of CmaCh04G014410 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.3e-47
Identity = 99/170 (58.24%), Postives = 123/170 (72.35%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           ++ K N   E AYGAGHINPL AVHPGL+Y+A E+DYV+FLCGQGY+T  +RRLS DN+ 
Sbjct: 531 LNAKLNTQVEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSV 590

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           C+  NSG V +LNYPSFALS+ +S   +Q FRR VTNVGS   ++Y+A +V   R L IT
Sbjct: 591 CTRANSGRVWDLNYPSFALSSTSSQSFNQFFRRTVTNVGS-KVSTYRAKVVGVPRGLSIT 650

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVF 171
           V P VL F  +G++ SF + I G I + I SA+LVW DG H VRSPITVF
Sbjct: 651 VNPPVLSFNAIGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVF 699

[Close]

BLAST of CmaCh04G014410 vs. TrEMBL
Match: A0A061FKX7_THECC (Subtilisin-like serine endopeptidase family protein isoform 2 OS=Theobroma cacao GN=TCM_042660 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.2e-45
Identity = 98/170 (57.65%), Postives = 122/170 (71.76%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           MS   N D E AYG+GH+NP+ AV PGL+Y++ E DY+KFLCGQGY T+ L+ ++ DNT+
Sbjct: 567 MSSGINLDAEFAYGSGHLNPIKAVDPGLVYDSEELDYIKFLCGQGYGTQFLQLVTRDNTT 626

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS   +GTV  LNYPSFAL T+AS  +S+VF R VTNVGS   + Y+A +  P   LKI 
Sbjct: 627 CSEATNGTVWGLNYPSFALFTSASTTVSRVFNRTVTNVGS-PMSIYRANVTAPAGALKIQ 686

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVF 171
           V P+VL FK LG++LSF + I G I K I SA+L WDDG HKVRSPIT F
Sbjct: 687 VNPNVLSFKSLGQKLSFSLTIEGTIDKSIVSASLEWDDGVHKVRSPITAF 735

[Close]

BLAST of CmaCh04G014410 vs. TAIR10
Match: AT3G46840.1 (AT3G46840.1 Subtilase family protein)

HSP 1 Score: 140.2 bits (352), Expect = 1.2e-33
Identity = 77/171 (45.03%), Postives = 107/171 (62.57%), Query Frame = 1


Query: 6   NPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANN 65
           N   E AYGAGH++P+ A+HPGL+Y A ++D++ FLCG  Y+ ++LR +S D++SC+   
Sbjct: 571 NELAEFAYGAGHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQ 630

Query: 66  SGTV-LELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPS 125
           + ++   LNYPS     +A+ P   +FRR VTNVG  +AT YKA +V     LK+ V P+
Sbjct: 631 TKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPA 690

Query: 126 VLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVFNAN 174
           VL  K L E+ SF V   G   K   + SA L+W DG H VRSPI V+  N
Sbjct: 691 VLSLKSLYEKKSFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVYATN 738

[Close]

BLAST of CmaCh04G014410 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 137.5 bits (345), Expect = 7.9e-33
Identity = 75/168 (44.64%), Postives = 105/168 (62.50%), Query Frame = 1


Query: 6   NPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANN 65
           N   E AYGAGH++P+ A+HPGL+Y A ++D++ FLCG  Y+ + LR +S D++SC+   
Sbjct: 571 NELAEFAYGAGHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQ 630

Query: 66  SGTVL-ELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPS 125
           + ++   LNYPS +   + + P    FRR VTNVG  +AT YKA +V     LK+ V P+
Sbjct: 631 TKSLTRNLNYPSMSAQVSGTKPFKVTFRRTVTNVGRPNAT-YKAKVVG--SKLKVKVVPA 690

Query: 126 VLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVF 171
           VL  K L E+ SF V + G   K   + SA L+W DG H VRSPI V+
Sbjct: 691 VLSLKSLYEKKSFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

[Close]

BLAST of CmaCh04G014410 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 131.3 bits (329), Expect = 5.7e-31
Identity = 71/173 (41.04%), Postives = 104/173 (60.12%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           M+ K+NP+ E AYG+G INP  A  PGL+Y     DY+K LC +G+ + +L   S  N +
Sbjct: 521 MNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVT 580

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS      V +LNYP+     ++ +P +  F+R VTNVG  ++T YKA++V     L+I+
Sbjct: 581 CSERTE--VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNST-YKASVVPLQPELQIS 640

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRK--GIESAALVWDDGKHKVRSPITVFN 172
           ++P +L F  L E+ SF V I GK  K     S+++VW DG H VRSPI  ++
Sbjct: 641 IEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYS 690

[Close]

BLAST of CmaCh04G014410 vs. TAIR10
Match: AT4G15040.1 (AT4G15040.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 130.2 bits (326), Expect = 1.3e-30
Identity = 73/174 (41.95%), Postives = 101/174 (58.05%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           M+  +N + E AYG+G +NP  AV PGL+Y   + DY+  LC   YS++ +  ++    +
Sbjct: 516 MNASKNAEAEFAYGSGFVNPTVAVDPGLVYEIAKEDYLNMLCSLDYSSQGISTIAGGTFT 575

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIV-NPWRILKI 120
           CS  +  T+  LNYPS +   +AS+     F R VTNVG   +T YKA +  NP   L I
Sbjct: 576 CSEQSKLTMRNLNYPSMSAKVSASSSSDITFSRTVTNVGEKGST-YKAKLSGNP--KLSI 635

Query: 121 TVKPSVLWFKRLGEELSFEVRIGGKIRKGIE---SAALVWDDGKHKVRSPITVF 171
            V+P+ L FK  GE+ SF V + GK   GI    SA+L+W DG H VRSPI V+
Sbjct: 636 KVEPATLSFKAPGEKKSFTVTVSGKSLAGISNIVSASLIWSDGSHNVRSPIVVY 686

[Close]

BLAST of CmaCh04G014410 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 127.1 bits (318), Expect = 1.1e-29
Identity = 66/164 (40.24%), Postives = 97/164 (59.15%), Query Frame = 1


Query: 10  ELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANNSGTV 69
           E AYGAGH++P+ A++PGL+Y   + D++ FLCG  Y++++L+ +S D   CS  N    
Sbjct: 566 EFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSKTLKIISGDTVKCSKKNKILP 625

Query: 70  LELNYPSF-ALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPSVLWF 129
             LNYPS  A  +   +  S  F R +TNVG+ ++T     +      L I V PSVL+F
Sbjct: 626 RNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKSKVVAGHGSKLSIKVTPSVLYF 685

Query: 130 KRLGEELSFEVRI-GGKIRKGIESAA-LVWDDGKHKVRSPITVF 171
           K + E+ SF V + G  +   + S+A L+W DG H VRSPI V+
Sbjct: 686 KTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGTHNVRSPIVVY 729

[Close]

BLAST of CmaCh04G014410 vs. NCBI nr
Match: gi|700197004|gb|KGN52181.1| (hypothetical protein Csa_5G613590 [Cucumis sativus])

HSP 1 Score: 255.4 bits (651), Expect = 7.4e-65
Identity = 126/174 (72.41%), Postives = 145/174 (83.33%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           MS K NPD E  YGAGHINPLGAVHPGLIY+A E DYV+FLCGQGY+TE L+ +S+DN +
Sbjct: 567 MSPKHNPDKEFGYGAGHINPLGAVHPGLIYDASEIDYVQFLCGQGYTTELLQLVSEDNNT 626

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS+NNS TV +LNYPSFALSTN S PI+QV++R VTNVGS  AT YKA ++NPW+ L+I 
Sbjct: 627 CSSNNSDTVFDLNYPSFALSTNISKPINQVYKRTVTNVGSKYAT-YKATVINPWKNLEIK 686

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANL 175
           V PSVL FK LGE+ SFEV I GKIRK IESA+LVWDDGKHKVRSPITVF AN+
Sbjct: 687 VNPSVLSFKNLGEKQSFEVTIRGKIRKDIESASLVWDDGKHKVRSPITVFIANI 739

[Close]

BLAST of CmaCh04G014410 vs. NCBI nr
Match: gi|659091821|ref|XP_008446751.1| (PREDICTED: cucumisin-like [Cucumis melo])

HSP 1 Score: 250.4 bits (638), Expect = 2.4e-63
Identity = 124/170 (72.94%), Postives = 143/170 (84.12%), Query Frame = 1


Query: 1    MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
            MS KRN + E AYGAGH+NPLGAVHPGLIY+A ETDYV+FLCGQGY+TE L+ +S+DN +
Sbjct: 1260 MSPKRNKEKEFAYGAGHLNPLGAVHPGLIYDASETDYVRFLCGQGYTTELLQLVSEDNNT 1319

Query: 61   CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
            CS+N+S TV +LNYPSFAL+TNAS PI+QV+RR VTNVGS SAT YKA I+NPW+ L+I 
Sbjct: 1320 CSSNDSDTVFDLNYPSFALATNASEPINQVYRRTVTNVGSKSAT-YKATIINPWKNLEIK 1379

Query: 121  VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVF 171
            V PSVL F  LGEE SFEV I GKI   IESA+LVWDDGKHKVRSPITVF
Sbjct: 1380 VNPSVLSFNHLGEEQSFEVTIKGKIMNDIESASLVWDDGKHKVRSPITVF 1428

[Close]

BLAST of CmaCh04G014410 vs. NCBI nr
Match: gi|778708693|ref|XP_004135365.2| (PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 2.9e-61
Identity = 121/174 (69.54%), Postives = 143/174 (82.18%), Query Frame = 1


Query: 1    MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
            MS KRN D E AYGAGH+NPLGAVHPGLIY+A E DYV+FLCGQGY+TE L+ +SD + +
Sbjct: 1267 MSPKRNQDKEFAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNT 1326

Query: 61   CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
            CS+N+S TV +LNYPSFALSTN S PI+QV+RR VTN+GS SA  YKA I+NPW+ L I 
Sbjct: 1327 CSSNDSDTVFDLNYPSFALSTNISVPINQVYRRTVTNIGSRSA-MYKATIINPWKNLDIK 1386

Query: 121  VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANL 175
            V PSVL F  LGE+ SFEV I GKIR+ IESA+LVW+DGKHKVRSPITVF+A +
Sbjct: 1387 VNPSVLSFTSLGEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVFDATI 1439

[Close]

BLAST of CmaCh04G014410 vs. NCBI nr
Match: gi|700197003|gb|KGN52180.1| (hypothetical protein Csa_5G613580 [Cucumis sativus])

HSP 1 Score: 243.4 bits (620), Expect = 2.9e-61
Identity = 121/174 (69.54%), Postives = 143/174 (82.18%), Query Frame = 1


Query: 1   MSHKRNPDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTS 60
           MS KRN D E AYGAGH+NPLGAVHPGLIY+A E DYV+FLCGQGY+TE L+ +SD + +
Sbjct: 567 MSPKRNQDKEFAYGAGHLNPLGAVHPGLIYDASEIDYVRFLCGQGYTTELLQLVSDGSNT 626

Query: 61  CSANNSGTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKIT 120
           CS+N+S TV +LNYPSFALSTN S PI+QV+RR VTN+GS SA  YKA I+NPW+ L I 
Sbjct: 627 CSSNDSDTVFDLNYPSFALSTNISVPINQVYRRTVTNIGSRSA-MYKATIINPWKNLDIK 686

Query: 121 VKPSVLWFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNANL 175
           V PSVL F  LGE+ SFEV I GKIR+ IESA+LVW+DGKHKVRSPITVF+A +
Sbjct: 687 VNPSVLSFTSLGEKQSFEVTIRGKIRRNIESASLVWNDGKHKVRSPITVFDATI 739

[Close]

BLAST of CmaCh04G014410 vs. NCBI nr
Match: gi|659074283|ref|XP_008437522.1| (PREDICTED: cucumisin-like isoform X3 [Cucumis melo])

HSP 1 Score: 205.3 bits (521), Expect = 8.8e-50
Identity = 106/167 (63.47%), Postives = 126/167 (75.45%), Query Frame = 1


Query: 7   PDGELAYGAGHINPLGAVHPGLIYNALETDYVKFLCGQGYSTESLRRLSDDNTSCSANNS 66
           P+ E AYG+GHINPLGAV+PGLIYNA E DY++FLCG+GY+T  LR ++ DN++CS  NS
Sbjct: 519 PEAEFAYGSGHINPLGAVNPGLIYNASEIDYIRFLCGEGYNTTLLRIITKDNSTCSPTNS 578

Query: 67  GTVLELNYPSFALSTNASNPISQVFRRRVTNVGSISATSYKAAIVNPWRILKITVKPSVL 126
           G V +LNY SFAL T  S P SQV++RRVTNVGS ++T YKA I  P   L ITV PS L
Sbjct: 579 GLVYDLNYHSFALFTYISTPFSQVYKRRVTNVGSTNST-YKATIFAPSE-LNITVNPSTL 638

Query: 127 WFKRLGEELSFEVRIGGKIRKGIESAALVWDDGKHKVRSPITVFNAN 174
            FK L EEL FEV I GKI   I SA+LVWDDG HKVRSPI VF+++
Sbjct: 639 SFKALEEELKFEVTIEGKINGSIASASLVWDDGVHKVRSPIIVFDSD 683

The following BLAST results are available for this feature:

BLAST of CmaCh04G014410 vs. Swiss-Prot


Match Name	E-value	Identity	Description
CUCM1_CUCME	5.2e-42	50.30	Cucumisin OS=Cucumis melo PE=1 SV=1	[more]
SBT45_ARATH	2.2e-32	45.03	Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana GN=SBT4.5 PE=3 SV=1	[more]
SBT46_ARATH	1.4e-31	44.64	Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1	[more]
SBT43_ARATH	1.0e-29	41.04	Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1	[more]
SBT42_ARATH	2.2e-29	41.95	Subtilisin-like protease SBT4.2 OS=Arabidopsis thaliana GN=SBT4.2 PE=3 SV=3	[more]

BLAST of CmaCh04G014410 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWL9_CUCSA	5.2e-65	72.41	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613590 PE=4 SV=1	[more]
A0A0A0KRC4_CUCSA	2.0e-61	69.54	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G613580 PE=4 SV=1	[more]
A0A0A0KJN5_CUCSA	5.2e-49	62.28	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G141040 PE=4 SV=1	[more]
A0A0A0KLR4_CUCSA	8.3e-47	58.24	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1	[more]
A0A061FKX7_THECC	1.2e-45	57.65	Subtilisin-like serine endopeptidase family protein isoform 2 OS=Theobroma cacao...	[more]

BLAST of CmaCh04G014410 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G46840.1	1.2e-33	45.03	Subtilase family protein	[more]
AT3G46850.1	7.9e-33	44.64	Subtilase family protein	[more]
AT5G59190.1	5.7e-31	41.04	subtilase family protein	[more]
AT4G15040.1	1.3e-30	41.95	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59090.1	1.1e-29	40.24	subtilase 4.12	[more]

BLAST of CmaCh04G014410 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700197004\|gb\|KGN52181.1\|	7.4e-65	72.41	hypothetical protein Csa_5G613590 [Cucumis sativus]	[more]
gi\|659091821\|ref\|XP_008446751.1\|	2.4e-63	72.94	PREDICTED: cucumisin-like [Cucumis melo]	[more]
gi\|778708693\|ref\|XP_004135365.2\|	2.9e-61	69.54	PREDICTED: LOW QUALITY PROTEIN: cucumisin-like [Cucumis sativus]	[more]
gi\|700197003\|gb\|KGN52180.1\|	2.9e-61	69.54	hypothetical protein Csa_5G613580 [Cucumis sativus]	[more]
gi\|659074283\|ref\|XP_008437522.1\|	8.8e-50	63.47	PREDICTED: cucumisin-like isoform X3 [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015500	Peptidase_S8_subtilisin-rel

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0004252	serine-type endopeptidase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G014410.1	CmaCh04G014410.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 1..173 score: 3.5
None	No IPR available	PANTHER	PTHR10795:SF348	SUBFAMILY NOT NAMED	coord: 1..173 score: 3.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G014410	ClCG05G021860	Watermelon (Charleston Gray)	cmawcgB655
CmaCh04G014410	CsaV3_5G035280	Cucumber (Chinese Long) v3	cmacucB0885

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G014410	Cucumber (Gy14) v1	cgycmaB0801
CmaCh04G014410	Cucurbita moschata (Rifu)	cmacmoB706
CmaCh04G014410	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G014410	Cucurbita moschata (Rifu)	cmacmoB740
CmaCh04G014410	Wild cucumber (PI 183967)	cmacpiB755
CmaCh04G014410	Cucumber (Chinese Long) v2	cmacuB748
CmaCh04G014410	Melon (DHL92) v3.5.1	cmameB640
CmaCh04G014410	Watermelon (Charleston Gray)	cmawcgB679
CmaCh04G014410	Watermelon (97103) v1	cmawmB667
CmaCh04G014410	Watermelon (97103) v1	cmawmB699
CmaCh04G014410	Cucurbita pepo (Zucchini)	cmacpeB700
CmaCh04G014410	Cucurbita pepo (Zucchini)	cmacpeB720
CmaCh04G014410	Cucurbita pepo (Zucchini)	cmacpeB734
CmaCh04G014410	Bottle gourd (USVL1VR-Ls)	cmalsiB669
CmaCh04G014410	Bottle gourd (USVL1VR-Ls)	cmalsiB706
CmaCh04G014410	Cucumber (Gy14) v2	cgybcmaB672
CmaCh04G014410	Melon (DHL92) v3.6.1	cmamedB726
CmaCh04G014410	Silver-seed gourd	carcmaB1170
CmaCh04G014410	Silver-seed gourd	carcmaB1457
CmaCh04G014410	Watermelon (97103) v2	cmawmbB758
CmaCh04G014410	Wax gourd	cmawgoB0903
CmaCh04G014410	Cucurbita maxima (Rimu)	cmacmaB402
CmaCh04G014410	Cucurbita maxima (Rimu)	cmacmaB536