CmaCh04G009640 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G009640
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionMethylenetetrahydrofolate reductase
LocationCma_Chr04 : 4943208 .. 4948570 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCCCGTTATTAATGAAATTATTTGTAAATCGGGAAATCGGGGGTTGGTATTCAATTCCTCGGCTAAGTGATGCCGATTCCGAGGTTGTTGGAGTGGGTGGGTTCCGAGACTACAACTGATCTCTTCGAATATTATTTATAGCTCAAACAGACAAATTCCCAGATGCGATTTCCTTCTCTCTCTCTCTCTCTTTCACCCATCTCCCTGTTTGGATCTCTCCATCTTCTGCTTTTCTCTCTTCGTTAGTCTTCAAATTTTCAATTCCTCCCATCTTTACTTCTGCCCATATGTTTATCATTGCTGATTTTTGCCATTGATTTGCAGACACAGACGCTCGCATTGCTCCCCATCTCTAACTGGTAAGATAGGATGAAGGTGATTGATAAGATCCGGGCAGCCACCACCGGCGATGATGCTAGAGTGGTGTTCTCGTTCGAGTACTTCCCGCCGAAGACGGAGGACGGTGTTGATAACCTCTTCGAGAGAATGGATCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCCGGTGGCTCCACTGCCGATCTCACACTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTCACCTGCACCAATATGCCTGTTGAGAAGATTGACCATGCTCTTCAAACCATCAAGTCCAATGGCATTCAGAATGTCCTCGCGCTTCGTGGTGACCCTCCCCATGGACAGGACAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTACTCTACATTCGTTTCGTCTCTGTTAGTTTTCTCTATTCTGATTTTCTGCTCTCTAAATGTTTCCGTTTCTTATTTCTAGGTTAAACACATCAGGGAGAAATATGGTGACTACTTTGGCATTACTGTAGCTGGATATCCAGGTCCTCTTTGTTTTGATCGGAAGTTACAGATCTACTTACAGATGGCTTTTCTTTTCTGTAACTAACGGAATGCTAATGGTTACTTCAATTCTCTTCTTCTCAGAGGCACATCCAGACGTGATTGGAAGCAACGGCGTGGCGACGCTTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGGTGACCCTGATCTTGAAGCAATTTTTAATCCCTGTTTCGCTGTTCTTACATGGTAACCTTTTATATTCTAATTTGTTTTATTTTTTGTTTTTTTCAGTTTGATGCTGGTGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGACAAATTGGAATAACTTGTCCGATTATACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCATACGCATGACCGAATTTTGCAAAACGAAGGTACTGAGTTCTTTCTGCTTCAACTGATCTCCTTTGACCCATATGTATTTGCTTGGAGTGAAAAATACTTCCATGTAAATTAACTTTGTTCGAACTGTTCTTCATACATTCTTGTGTTCTGCTTTCTCAAATTTCTTGTGTTAAGGAAGAAATGTCTCAATGGTTTCTATGTTGATGCAAACAATACTTTCAAGTCTTGATGCTGTTTGATAGTTGTGATTAGAAGAATCCAGCTATTGCCTGCCACTTTCAAACATCTTCTTTGGCAATATCGGTCACTTTAAATTCATACTTTTTTCTAAATTCTTTCAAGCCTTGATGCTGTTTGATAATTGTAATGTCTAATCACATATGCTCCTGTATTTTCTAAATTCATACTTCTCTCATCAGATTATCCATAATAGCCTTGATATAAATCATATGCGTCGTTATTAATTGGTCACCTGCTCTATTCCTAGAAATTTACAATCAGTGATCATCAAATTTTCATTTTTCTTTAAAGAGATAATTCGATCTATAATGTTAAATTGGTTCTCTTTTACCTGTATGTATGACTTTTGAAAACTTGCAGGTTCCGGATGAGATCATGGCTGCCTTAGAGCCTATCAAGGACAATGAAGAGGCTGTTAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAAACACTACATCTTTATACGCTGAATATGGAGAAATCAGCATTGGCCATACTAATGGTCCATTCCATTATCCCGTTAACATCTTAGTTTTACTTCCAACCATCGTTTCTATGGGTTATTTGTCTGTGGATGCTCTGTTACCAGGGAAATTGCTGAAGTTTTTTTTTGTTAATGTATTCTCATTTTCGGGGCGGATTGCATTTGATAGAATTTGGCTTCCTTTCCTTTCTCATTCTTGATTCATTATTATTTGGACTACTGAGACTGCATTTGCGTTTTGAAGTCAACTTGAAAGAAGCTTTTAAGTAATTTTTGTAATGAAAACTTCTGAATGTAATTGCTAATGAGTCTCGTTAAAAGTAAGTTATGGAAACAGTGATACTATTACTAACCTATTTCTTGATTAACGTTGAATAAACTTTTCTTATACTCACAGGAACATGACTCGTCCGTCCGCACAGTTTTTAAGATAAAATTAATAGTACATATAATATAACATTAGATAGTTTTTTTGTTGATTGGATGTAGTTATCTTTTTCTGATAATATAGACTTTGTTATTCTTTTTTTTTTTCCATACGTTATCATTATTTTATTTTCATTTGCTCAGGCTTGTAGTATATTCTGTGCTTTTGGGGTTTAATTCATGTCCAAGTGCACTTTTACTCATTTATATTTGAACATATGACAGAGTCTTGGTCTGATTGAAGAAAGCAAAATATCAAGATCTTTACCTTGGAGGCGCCCAACAAATATTTTCCGTGTCCATGAAGATGTTCGCCCAATCTTCTGGTATGTGATATGTTTTTTTGAAAATTTGGCACATATCCCTAATTATTTAGAATGCATAAAATTTTTCTACACTTGGTTAAATTTGAGATAGAGATGATCTATATAGTTGACGATTGCAGGGCAAACCGTCCTAAAAGCTACATATCAAGGACCATAGGTTGGGATCAGTACCCTCATGGTAGATGGACTGATTCTAGGAACCCTTCATATGGGGCACTAACTGACTATCAGGTATTAACAATAGTACTTGTAAACTGCATCTTCTATGTGAATGGTATTTGTTAATACATGTTCTATGTTAATAATATTTGTTTTATATATATATATGCATACACTCTAGAGAGGGAGCTAGAGAAAAAGAGAGAGACAGGCAGCTGTAAGGAGAAAATTCCTTTATAACCTCCGGAATAAATTCTTGCTGACTACCTGTTAGAACTTGAATCTTGCATTCCCCCTTTGATGACTGTAGTCTCATTATTGAACTCCTTGGGATCTGCATGAATTAGAGATTAAAAGAAGCTATCCTTGGTAAACTTTTTTCCGCATAATGTAACTTTTACTTTTGGCTTGGTACGATTAGTCTGACACCATTATCAAAATAGCATTCTGTTGAGTGGAATGAAACTTATTCTAGGAACATATATTAATTGTAAAAAAAAAAAAAAAAAAAAGCTACCAAATCCTTTTGAAAAGTAGTGGCAGGCTTTTCCGTGTAAATTTGTGCATTCCTTTTTATTGTTGCTTGTAAATTGTGTATTCATTTTCATTGTTACTATACGTGAGCTGGCGAGATGATTTATTCTCTCTTTTTTAAGCACTTCAAAAAGCTGAAAACATTAACTTCCATTCATTCAATTTTTATTTGGACATTGCTGCAACACTTTCAATATGGTTTCTAAAGCATTCAAGTGTAACCAGTTTTATTTTTGCTTTTTTTCACTTAAGTTTATGCGCCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTTCCATTGAATAGCATAGAAGATGTTTATGAGGTACTACTGCTGCCTTCTAACCAAGAGTGTAAAGAAATTTTGTTATATCAATATGATGCAATATGCAGAACCTTAGATGTGTTTGGCTGTTTCATATCAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCATGGTCTGAATTAGACGGACTTCAGGCTGAGACAAGGATAATAAGTGAACAGCTTGGAAAAATTAACATGAAAGGTCTCCTTACCATCAACAGCCAACCATCGGTTAACGGAGAAAGATCTGATTCTCCTTCTGTAGGTAAATTCAGATTGATGCATTCATCGAATTAATTTTTGATTATTAATATATTAGTCTTTCCCCTTTGACTGGCTAGCAACTTAATAAGTTAATGTATAAAGAATAATAAATGGTTTAAATCCTATTTCCTAAACTTTACATTTAAAAAACTAAATCTATTTCAGTTCTGACTTCTGTGATTTGTGGTATTGGCGTCAGAATGCTTATTTAGTCATCTGACCAGTGTTGGGACGATTGATAATGATTTACAGGATGGGGTGGATCAGGAGGTTACGTCTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAAAGAAAAGTTGGATGCTGTTGTTGACAAGTGCAGGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAAGAAGGCGTTTGGGTATCCAACATCACCCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATACAGCCAACCGTCGTCGATCCTGCAAGCTTTCACGTGTGGAAAGACGAGGCATTTGAGCTTTGGTCGAGGGGATGGGCTTCCCTGTACCCTGAGGGAGACCCGTCCAGAAAATTACTCGAGGAGGTTGGTACCTAATTACATCTTAATCAATGTTTATGGTGCAGTTGTTGACAAGTATTTTGAATTTGACAGTATTCCTCCAAATTTCTGTTTACGATTTATTTCAGATACAGATCAGCTATTACTTGGTGAGCTTGGTGGATAACGACTACGTCAATTCTAACATCTTTGCTGCTTTGGAGGATTTCTGAGATGAGCTGCTCTGCCTTTTCATGATTCTAGTTTTACATCCATCCATCTTTGGATCTCTTATTCAAACCTGGATATGGTTAATTCAAGAAGCATTTTGTTTGTCCGTTTGTTTCAGTTTATAATATAATTTCATCAATTTTTGGAGTGTAAAATGTATATCATTTATTTGTGACTGTGGAAGCATTTTATTAAAATAATTTTGAAGGTAGCCATATCCTTGGCATGGGATATCATATTATACCATTTCATCATATGGTTTGCTTTTAACATGTTTGTCATTTGGAAATTTAAGTGATTGCCTAATCTCAATTGGGACAAGATGGTTTTTGTCCCTTCGAATTTGGTTAACATTTGAGCTGCAGTTAGTGGAAGTTGGTTAGTAAGGTCAGACACCAGAACGTAAGGGGATTCTTTTCTATGTTCTT

mRNA sequence

CAACCCCGTTATTAATGAAATTATTTGTAAATCGGGAAATCGGGGGTTGGTATTCAATTCCTCGGCTAAGTGATGCCGATTCCGAGGTTGTTGGAGTGGGTGGGTTCCGAGACTACAACTGATCTCTTCGAATATTATTTATAGCTCAAACAGACAAATTCCCAGATGCGATTTCCTTCTCTCTCTCTCTCTCTTTCACCCATCTCCCTGTTTGGATCTCTCCATCTTCTGCTTTTCTCTCTTCACACAGACGCTCGCATTGCTCCCCATCTCTAACTGGTAAGATAGGATGAAGGTGATTGATAAGATCCGGGCAGCCACCACCGGCGATGATGCTAGAGTGGTGTTCTCGTTCGAGTACTTCCCGCCGAAGACGGAGGACGGTGTTGATAACCTCTTCGAGAGAATGGATCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCCGGTGGCTCCACTGCCGATCTCACACTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTCACCTGCACCAATATGCCTGTTGAGAAGATTGACCATGCTCTTCAAACCATCAAGTCCAATGGCATTCAGAATGTCCTCGCGCTTCGTGGTGACCCTCCCCATGGACAGGACAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACACATCAGGGAGAAATATGGTGACTACTTTGGCATTACTGTAGCTGGATATCCAGAGGCACATCCAGACGTGATTGGAAGCAACGGCGTGGCGACGCTTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGTTTGATGCTGGTGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGACAAATTGGAATAACTTGTCCGATTATACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCATACGCATGACCGAATTTTGCAAAACGAAGGTTCCGGATGAGATCATGGCTGCCTTAGAGCCTATCAAGGACAATGAAGAGGCTGTTAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAAACACTACATCTTTATACGCTGAATATGGAGAAATCAGCATTGGCCATACTAATGAGTCTTGGTCTGATTGAAGAAAGCAAAATATCAAGATCTTTACCTTGGAGGCGCCCAACAAATATTTTCCGTGTCCATGAAGATGTTCGCCCAATCTTCTGGGCAAACCGTCCTAAAAGCTACATATCAAGGACCATAGGTTGGGATCAGTACCCTCATGGTAGATGGACTGATTCTAGGAACCCTTCATATGGGGCACTAACTGACTATCAGTTTATGCGCCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTTCCATTGAATAGCATAGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCATGGTCTGAATTAGACGGACTTCAGGCTGAGACAAGGATAATAAGTGAACAGCTTGGAAAAATTAACATGAAAGGTCTCCTTACCATCAACAGCCAACCATCGGTTAACGGAGAAAGATCTGATTCTCCTTCTGTAGGATGGGGTGGATCAGGAGGTTACGTCTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAAAGAAAAGTTGGATGCTGTTGTTGACAAGTGCAGGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAAGAAGGCGTTTGGGTATCCAACATCACCCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATACAGCCAACCGTCGTCGATCCTGCAAGCTTTCACGTGTGGAAAGACGAGGCATTTGAGCTTTGGTCGAGGGGATGGGCTTCCCTGTACCCTGAGGGAGACCCGTCCAGAAAATTACTCGAGGAGATACAGATCAGCTATTACTTGGTGAGCTTGGTGGATAACGACTACGTCAATTCTAACATCTTTGCTGCTTTGGAGGATTTCTGAGATGAGCTGCTCTGCCTTTTCATGATTCTAGTTTTACATCCATCCATCTTTGGATCTCTTATTCAAACCTGGATATGGTTAATTCAAGAAGCATTTTGTTTGTCCGTTTGTTTCAGTTTATAATATAATTTCATCAATTTTTGGAGTGTAAAATGTATATCATTTATTTGTGACTGTGGAAGCATTTTATTAAAATAATTTTGAAGGTAGCCATATCCTTGGCATGGGATATCATATTATACCATTTCATCATATGGTTTGCTTTTAACATGTTTGTCATTTGGAAATTTAAGTGATTGCCTAATCTCAATTGGGACAAGATGGTTTTTGTCCCTTCGAATTTGGTTAACATTTGAGCTGCAGTTAGTGGAAGTTGGTTAGTAAGGTCAGACACCAGAACGTAAGGGGATTCTTTTCTATGTTCTT

Coding sequence (CDS)

ATGAAGGTGATTGATAAGATCCGGGCAGCCACCACCGGCGATGATGCTAGAGTGGTGTTCTCGTTCGAGTACTTCCCGCCGAAGACGGAGGACGGTGTTGATAACCTCTTCGAGAGAATGGATCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCCGGTGGCTCCACTGCCGATCTCACACTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTCGAGACCATGATGCATCTCACCTGCACCAATATGCCTGTTGAGAAGATTGACCATGCTCTTCAAACCATCAAGTCCAATGGCATTCAGAATGTCCTCGCGCTTCGTGGTGACCCTCCCCATGGACAGGACAAATTCGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACACATCAGGGAGAAATATGGTGACTACTTTGGCATTACTGTAGCTGGATATCCAGAGGCACATCCAGACGTGATTGGAAGCAACGGCGTGGCGACGCTTGAAGGCTACCAGAGCGATCTGGCTTATCTTAAGAGAAAGTTTGATGCTGGTGCTGATCTGATTGTTACCCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGACAAATTGGAATAACTTGTCCGATTATACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCATACGCATGACCGAATTTTGCAAAACGAAGGTTCCGGATGAGATCATGGCTGCCTTAGAGCCTATCAAGGACAATGAAGAGGCTGTTAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGCAAGAAGATTTTGGCTCATGGAATCAAAACACTACATCTTTATACGCTGAATATGGAGAAATCAGCATTGGCCATACTAATGAGTCTTGGTCTGATTGAAGAAAGCAAAATATCAAGATCTTTACCTTGGAGGCGCCCAACAAATATTTTCCGTGTCCATGAAGATGTTCGCCCAATCTTCTGGGCAAACCGTCCTAAAAGCTACATATCAAGGACCATAGGTTGGGATCAGTACCCTCATGGTAGATGGACTGATTCTAGGAACCCTTCATATGGGGCACTAACTGACTATCAGTTTATGCGCCCACGTGCACGTGACAAGAAGCTTCTTGAGGAATGGGCTCTTCCATTGAATAGCATAGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCATGGTCTGAATTAGACGGACTTCAGGCTGAGACAAGGATAATAAGTGAACAGCTTGGAAAAATTAACATGAAAGGTCTCCTTACCATCAACAGCCAACCATCGGTTAACGGAGAAAGATCTGATTCTCCTTCTGTAGGATGGGGTGGATCAGGAGGTTACGTCTATCAAAAGGCATACATCGAGTTTTTCTGTTCCAAAGAAAAGTTGGATGCTGTTGTTGACAAGTGCAGGGCTCTTCCATCTCTAACCTACATGGCGGTGAATAAAGAAGGCGTTTGGGTATCCAACATCACCCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATACAGCCAACCGTCGTCGATCCTGCAAGCTTTCACGTGTGGAAAGACGAGGCATTTGAGCTTTGGTCGAGGGGATGGGCTTCCCTGTACCCTGAGGGAGACCCGTCCAGAAAATTACTCGAGGAGATACAGATCAGCTATTACTTGGTGAGCTTGGTGGATAACGACTACGTCAATTCTAACATCTTTGCTGCTTTGGAGGATTTCTGA

Protein sequence

MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAYLKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCKTKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILMSLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSRNPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQAETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEKLDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHVWKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF
BLAST of CmaCh04G009640 vs. Swiss-Prot
Match: MTHR2_ARATH (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 1040.0 bits (2688), Expect = 9.8e-303
Identity = 482/595 (81.01%), Postives = 539/595 (90.59%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI++    D+ +  FSFE+FPPKTEDGVDNLFERMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQSLA--DEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPI+PGIMPINNY+GF+RMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P E+MAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LHLYTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LG+I+ESKISRSLPWRRP N+FR  EDVRPIFWANRPKSYISRT GW+ +P GRW DSR
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PL S+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ETRII+EQL K+N KG LTINSQPSVN ERSDSP+VGWGG  GYVYQKAY+EFFCSKEK
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDAVV+KC+ALPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF+V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALED 596
           WKDEAFE WSR WA+LYPE DPSR LLEE++ SYYLVSLV+NDY+N +IFA   D
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

BLAST of CmaCh04G009640 vs. Swiss-Prot
Match: MTHR_ORYSJ (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 8.6e-299
Identity = 482/595 (81.01%), Postives = 532/595 (89.41%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI+ A    D R VFSFEYFPPKTE+G+DNLFERMDRMVAH P+FCDITWGAGGS
Sbjct: 1   MKVIEKIQEAAA--DGRTVFSFEYFPPKTEEGLDNLFERMDRMVAHGPNFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKID AL TIKSNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDDALTTIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+ GGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I S   AT E Y +DLAY
Sbjct: 121 DKFVQVAGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+K DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 181 LKQKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P EI AALEPIKDNEEAVKAYGIHLGTEMCKKILA GIKTLHLYTLNMEKSAL ILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESKISRSLPWR PTN+FRV EDVRPIFWANRPKSYISRT+GWDQYPHGRW DSR
Sbjct: 301 NLGLIEESKISRSLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQF RPR R KKL EEWA+P+ S+ED+ E+FM +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDYQFTRPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II +QL KIN KG LTINSQP+VNGERSDS SVGWGG GGYVYQKAY+EFFCSKEK
Sbjct: 421 PETKIIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LD +++K +A PSLTY+AVNK+G   SNI    VNAVTWGVFP KEI+QPTVVD ASF V
Sbjct: 481 LDQLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALED 596
           WKDEAFE+WS+GWA L+PEGD SR++L+++Q SY+LVSLVDNDY+N ++FAA ++
Sbjct: 541 WKDEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKE 593

BLAST of CmaCh04G009640 vs. Swiss-Prot
Match: MTHR1_MAIZE (Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 5.2e-296
Identity = 474/594 (79.80%), Postives = 527/594 (88.72%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A    D R  FSFEYFPPKTE+GV+NLFERMDRMVAH PSFCDITWGAGGS
Sbjct: 1   MKVIEKILEAA--GDGRTAFSFEYFPPKTEEGVENLFERMDRMVAHGPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKIDHAL+TIKSNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I   G ATLE Y +DLAY
Sbjct: 121 DKFVQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LKRK DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 181 LKRKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P EI AAL+PIKDNEEAV+ YGIHLGTEMCKKILA GIKTLHLYTLNM+KSA+ ILM
Sbjct: 241 TKIPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESK+SR LPWR  TN+FRV EDVRPIFWANRPKSY+ RT+GWDQYPHGRW DSR
Sbjct: 301 NLGLIEESKVSRPLPWRPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTD+QF RPR R KKL EEWA+PL S+ED+ E+F  +C GKL SSPWSELDGLQ
Sbjct: 361 NPSYGALTDHQFTRPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II +QL  IN KG LTINSQP+VNGE+SDSP+VGWGG GGYVYQKAY+EFFC+KEK
Sbjct: 421 PETKIIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LD +++K +A PSLTY+AVNK+G   SNI+   VNAVTWGVFP KEIIQPTVVD ASF V
Sbjct: 481 LDQLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALE 595
           WKDEAFE+W+RGW  ++PEGD SR+LLE++Q +YYLVSLVDNDYV  ++FAA +
Sbjct: 541 WKDEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFAAFK 592

BLAST of CmaCh04G009640 vs. Swiss-Prot
Match: MTHR1_ARATH (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 1004.2 bits (2595), Expect = 6.0e-292
Identity = 459/591 (77.66%), Postives = 528/591 (89.34%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  +  +  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPI+PGIMPI+NYKGF+RM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLI+ESKISRSLPWRRP N+FR  EDVRPIFWANRPKSYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PL SIEDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+EQLGKIN  G LTINSQPSVN  +SDSP++GWGG GGYVYQKAY+EFFCSK+K
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LD +V+K +A PS+TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFA 592
           WKDEAFE+WSR WA+LYPE DPSRKLLEE++ SYYLVSLVDN+Y+N +IF+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

BLAST of CmaCh04G009640 vs. Swiss-Prot
Match: MTHR_HUMAN (Methylenetetrahydrofolate reductase OS=Homo sapiens GN=MTHFR PE=1 SV=3)

HSP 1 Score: 532.3 bits (1370), Expect = 6.6e-150
Identity = 267/592 (45.10%), Postives = 376/592 (63.51%), Query Frame = 1

Query: 20  FSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGSTAD----LTLDIANKMQNM 79
           FS E+FPP+T +G  NL  R DRM A  P + D+TW   G         ++ IA+   N 
Sbjct: 60  FSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIASTAVNY 119

Query: 80  ICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQDKFVQIEGGFACALD 139
             +ET++H+TC    +E+I   L   K  G++N++ALRGDP    D++ + EGGF  A+D
Sbjct: 120 CGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPIG--DQWEEEEGGFNYAVD 179

Query: 140 LVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAYLKRKFDAGADLIVTQ 199
           LVKHIR ++GDYF I VAGYP+ HP+  GS        +++DL +LK K  AGAD I+TQ
Sbjct: 180 LVKHIRSEFGDYFDICVAGYPKGHPEA-GS--------FEADLKHLKEKVSAGADFIITQ 239

Query: 200 LFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCKTKVPDEIMAALEPIK 259
           LF++ D F +FV  C  +GITCPI+PGI PI  Y    ++ +  K +VP EI   +EPIK
Sbjct: 240 LFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKDVIEPIK 299

Query: 260 DNEEAVKAYGIHLGTEMCKKILAHG-IKTLHLYTLNMEKSALAILMSLGLIEESKISRSL 319
           DN+ A++ YGI L   +C+++LA G +  LH YTLN E +   +L  LG+  E    R L
Sbjct: 300 DNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEDP-RRPL 359

Query: 320 PWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSRNPSYGALTDYQ--F 379
           PW    +  R  EDVRPIFWA+RPKSYI RT  WD++P+GRW +S +P++G L DY   +
Sbjct: 360 PWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFY 419

Query: 380 MRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLG-------KLRSSPWSELDGLQAETRI 439
           ++ ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ + L AET +
Sbjct: 420 LKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPLAAETSL 479

Query: 440 ISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEKLDAVV 499
           + E+L ++N +G+LTINSQP++NG+ S  P VGWG SGGYV+QKAY+EFF S+E  +A++
Sbjct: 480 LKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALL 539

Query: 500 DKCRALP-SLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHVWKDE 559
              +     + Y  VN +G  ++N  +   NAVTWG+FP +EIIQPTVVDP SF  WKDE
Sbjct: 540 QVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDE 599

Query: 560 AFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSN-IFAALED 596
           AF LW   W  LY E  PSR +++ I  +Y+LV+LVDND+   N ++  +ED
Sbjct: 600 AFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVED 638

BLAST of CmaCh04G009640 vs. TrEMBL
Match: A0A0A0LIC6_CUCSA (Methylenetetrahydrofolate reductase OS=Cucumis sativus GN=Csa_2G149470 PE=3 SV=1)

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 556/596 (93.29%), Postives = 580/596 (97.32%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAAT GD  RVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNG+QNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+KFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+PDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLH+YTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESK+SRSLPWRRPTN+FRV EDVRPIFWANRP+SYISRTIGWDQYPHGRW+DSR
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPL SIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
           AETR+ISEQLGKINMKG L+INSQP+VNGERSDSP VGWGG GGYVYQKAYIEFFCS+EK
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+VDKCRALPSLTYMAVNKEG+WVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWA+LYPEGD SRKLL+EIQ SYYLVSLVDNDY+NS++FA LEDF
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of CmaCh04G009640 vs. TrEMBL
Match: U5FIP4_POPTR (Methylenetetrahydrofolate reductase OS=Populus trichocarpa GN=POPTR_0017s00520g PE=3 SV=1)

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 507/604 (83.94%), Postives = 551/604 (91.23%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDA---------RVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFC 60
           MKVIDKIRAA    D+         +VVFSFE+FPPKT+DGVDNLFERMDRMV+HNPSFC
Sbjct: 1   MKVIDKIRAAAAAGDSNGGDSSSNNKVVFSFEFFPPKTDDGVDNLFERMDRMVSHNPSFC 60

Query: 61  DITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLA 120
           DITWGAGGSTADLTLDIANKMQN+ICVETMMHLTCTNMPVEKIDHAL+TIKSNGIQNVLA
Sbjct: 61  DITWGAGGSTADLTLDIANKMQNIICVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLA 120

Query: 121 LRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATL 180
           LRGDPPHGQDKFVQI+GG ACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGS+G ATL
Sbjct: 121 LRGDPPHGQDKFVQIQGGLACALDLVKHIRSKYGDYFGITVAGYPEAHPDVIGSDGFATL 180

Query: 181 EGYQSDLAYLKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKG 240
           E Y  DLAYLK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPI+PGIMPINNYKG
Sbjct: 181 EDYHKDLAYLKQKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPINNYKG 240

Query: 241 FIRMTEFCKTKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNM 300
           FIRM  FCKTK+P E+ AALEPIKDNEEAV+AYGIHLGTEMCKKILAHGIKTLHLYTLNM
Sbjct: 241 FIRMAGFCKTKIPAEVTAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNM 300

Query: 301 EKSALAILMSLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQY 360
           EKSALAILM+LGLIEESKI+R LPWR PTN+FRV EDVRPIFWANRPKSY++RTIGWDQY
Sbjct: 301 EKSALAILMNLGLIEESKITRPLPWRPPTNVFRVKEDVRPIFWANRPKSYLTRTIGWDQY 360

Query: 361 PHGRWTDSRNPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSS 420
           PHGRW DSRNPSYGAL+DYQFMRPRAR KKLLEEWA PL S+ED+YEKF  YCLGKL+SS
Sbjct: 361 PHGRWGDSRNPSYGALSDYQFMRPRARGKKLLEEWAAPLKSVEDIYEKFKVYCLGKLKSS 420

Query: 421 PWSELDGLQAETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAY 480
           PWSELDGLQ ET+II+EQLGKIN+KG LTINSQP+VNGE+SDSPSVGWGG GGYVYQKAY
Sbjct: 421 PWSELDGLQPETKIINEQLGKINLKGFLTINSQPAVNGEKSDSPSVGWGGPGGYVYQKAY 480

Query: 481 IEFFCSKEKLDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPT 540
           +EFFCSK+KL+A+VDKC++ P +TY+AVNK G W+SN+   DVNAVTWGVFP KEIIQPT
Sbjct: 481 LEFFCSKDKLNALVDKCKSFPFVTYIAVNKGGSWISNVALTDVNAVTWGVFPGKEIIQPT 540

Query: 541 VVDPASFHVWKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFA 596
           VVDP SF VWKDEAFE+WSRGWASLYPEGDPSR LLEE+Q SY+LVSLVDNDY++ +IFA
Sbjct: 541 VVDPTSFSVWKDEAFEIWSRGWASLYPEGDPSRTLLEEVQSSYFLVSLVDNDYIHGDIFA 600

BLAST of CmaCh04G009640 vs. TrEMBL
Match: A0A0B2SRC1_GLYSO (Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_018451 PE=3 SV=1)

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 505/596 (84.73%), Postives = 555/596 (93.12%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KIRAA+  D  RVVFSFE+FPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIRAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVI S+G+AT EGY++DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGFIRMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P +IMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESK+SRSLPWRRP N+FRV EDVRPIFWANRPKSYISRTIGWDQYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+PL S+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+EQL KIN KG LTINSQP+VNGE+SDSP+VGWGG GGYVYQKAY+EFFCSKEK
Sbjct: 421 PETKIINEQLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEIIQPT+VDP SF+V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWASLYPE + SRKL+EE+  S++LVSLVDNDY+N ++FA   DF
Sbjct: 541 WKDEAFEIWSRGWASLYPEDEASRKLVEEVGGSHFLVSLVDNDYINGDLFAVFADF 595

BLAST of CmaCh04G009640 vs. TrEMBL
Match: I1JZE2_SOYBN (Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_04G256100 PE=3 SV=1)

HSP 1 Score: 1083.9 bits (2802), Expect = 0.0e+00
Identity = 505/596 (84.73%), Postives = 555/596 (93.12%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MK+I+KIRAA+  D  RVVFSFE+FPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKIIEKIRAASA-DPNRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN++CVETMMHLTCTNMPVEKIDHAL TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIVCVETMMHLTCTNMPVEKIDHALHTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVI S+G+AT EGY++DLAY
Sbjct: 121 DKFVQVEGGFACARDLVQHIRAKYGDYFGITIAGYPEAHPDVIESDGLATPEGYRNDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGFIRMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P +IMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM
Sbjct: 241 TKIPADIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESK+SRSLPWRRP N+FRV EDVRPIFWANRPKSYISRTIGWDQYPHGRW DS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPANVFRVKEDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSC 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRARDKKL+EEWA+PL S+ED+YE+F  YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARDKKLVEEWAVPLKSVEDIYERFRLYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+EQL KIN KG LTINSQP+VNGE+SDSP+VGWGG GGYVYQKAY+EFFCSKEK
Sbjct: 421 PETKIINEQLEKINTKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+VDKC+   SLTYMAVNK+G W SN+ Q DVNAVTWGVFPAKEIIQPT+VDP SF+V
Sbjct: 481 LDALVDKCKDRTSLTYMAVNKDGSWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWASLYPE + SRKL+EE+  S++LVSLVDNDY+N ++FA   DF
Sbjct: 541 WKDEAFEIWSRGWASLYPEDEASRKLVEEVGGSHFLVSLVDNDYINGDLFAVFADF 595

BLAST of CmaCh04G009640 vs. TrEMBL
Match: A0A072VS77_MEDTR (Methylenetetrahydrofolate reductase OS=Medicago truncatula GN=MTR_1g040105 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 504/596 (84.56%), Postives = 553/596 (92.79%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIR+A   D  +VVFSFE+FPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGG+
Sbjct: 1   MKVIDKIRSANA-DPNKVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGT 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IANKMQN+ICVETMMHLTCTNMPV+KIDHAL+TIKSNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNIICVETMMHLTCTNMPVDKIDHALETIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           +KFVQ EGGFACA DLV+HIR KYGDYFGIT+AGYPEAHPDVIG++G+AT EGYQSDLAY
Sbjct: 121 EKFVQTEGGFACARDLVQHIRSKYGDYFGITIAGYPEAHPDVIGADGLATAEGYQSDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGFIRMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFIRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P EIMAALEPIKDNEEAVKAYGIHLGTEMCKKI+AHGIKT+HLYTLNMEKSALAIL 
Sbjct: 241 TKIPAEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIMAHGIKTVHLYTLNMEKSALAILT 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           SLGLIEESKISRSLPWRRPTN+FRV EDVRPIFWANRPKSYISRTIGW+QYPHGRW+DS 
Sbjct: 301 SLGLIEESKISRSLPWRRPTNVFRVKEDVRPIFWANRPKSYISRTIGWEQYPHGRWSDSG 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRA+DKKL+EEWA+PL SIED+YE+F  +CLGKLR++PWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRAKDKKLIEEWAVPLKSIEDIYERFRLFCLGKLRTNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+E L KIN KG LTINSQP+VNGE+SDSP VGWGG GGY+YQKAY+EFFCSKEK
Sbjct: 421 PETKIINEHLEKINTKGFLTINSQPAVNGEKSDSPIVGWGGPGGYIYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+++K +  P LTYMAVNKEG+W SN+ Q DVNAVTWGVFPAKEIIQPT+VDP SF+V
Sbjct: 481 LDALIEKSKDRPFLTYMAVNKEGIWKSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFNV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWASLYPE D SRKL+EE+  SY+LVSLVDNDY N ++F  L DF
Sbjct: 541 WKDEAFEIWSRGWASLYPEADASRKLVEEVGSSYFLVSLVDNDYANGDLFGVLADF 595

BLAST of CmaCh04G009640 vs. TAIR10
Match: AT2G44160.1 (AT2G44160.1 methylenetetrahydrofolate reductase 2)

HSP 1 Score: 1040.0 bits (2688), Expect = 5.5e-304
Identity = 482/595 (81.01%), Postives = 539/595 (90.59%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI++    D+ +  FSFE+FPPKTEDGVDNLFERMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQSLA--DEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI+SNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPI+PGIMPINNY+GF+RMT FCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P E+MAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LHLYTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LG+I+ESKISRSLPWRRP N+FR  EDVRPIFWANRPKSYISRT GW+ +P GRW DSR
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PL S+ED+ EKF + CLG L+SSPWSELDGLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ETRII+EQL K+N KG LTINSQPSVN ERSDSP+VGWGG  GYVYQKAY+EFFCSKEK
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDAVV+KC+ALPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF+V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALED 596
           WKDEAFE WSR WA+LYPE DPSR LLEE++ SYYLVSLV+NDY+N +IFA   D
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

BLAST of CmaCh04G009640 vs. TAIR10
Match: AT3G59970.3 (AT3G59970.3 methylenetetrahydrofolate reductase 1)

HSP 1 Score: 1004.2 bits (2595), Expect = 3.4e-293
Identity = 459/591 (77.66%), Postives = 528/591 (89.34%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  +  +  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI+SNGIQNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPI+PGIMPI+NYKGF+RM  FCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LHLYTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLI+ESKISRSLPWRRP N+FR  EDVRPIFWANRPKSYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PL SIEDV EKF + C+G L+SSPWSELDGLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+EQLGKIN  G LTINSQPSVN  +SDSP++GWGG GGYVYQKAY+EFFCSK+K
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LD +V+K +A PS+TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFA 592
           WKDEAFE+WSR WA+LYPE DPSRKLLEE++ SYYLVSLVDN+Y+N +IF+
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFS 589

BLAST of CmaCh04G009640 vs. NCBI nr
Match: gi|778668497|ref|XP_004138970.2| (PREDICTED: methylenetetrahydrofolate reductase 2 [Cucumis sativus])

HSP 1 Score: 1180.2 bits (3052), Expect = 0.0e+00
Identity = 556/596 (93.29%), Postives = 580/596 (97.32%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAAT GD  RVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNG+QNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK+KFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+PDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLH+YTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESK+SRSLPWRRPTN+FRV EDVRPIFWANRP+SYISRTIGWDQYPHGRW+DSR
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPL SIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
           AETR+ISEQLGKINMKG L+INSQP+VNGERSDSP VGWGG GGYVYQKAYIEFFCS+EK
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+VDKCRALPSLTYMAVNKEG+WVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWA+LYPEGD SRKLL+EIQ SYYLVSLVDNDY+NS++FA LEDF
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of CmaCh04G009640 vs. NCBI nr
Match: gi|1009142164|ref|XP_015888576.1| (PREDICTED: methylenetetrahydrofolate reductase 2-like [Ziziphus jujuba])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 515/596 (86.41%), Postives = 558/596 (93.62%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI+ A+  D+ +VVFSFE+FPPKTEDGVDNLFERMDRMVAHNP+FCDITWGAGGS
Sbjct: 1   MKVIEKIQDASL-DEKKVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTLDIAN+MQN+ICVE+MMHLTCTNMPVEKIDHAL TIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANRMQNIICVESMMHLTCTNMPVEKIDHALDTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQ EGGFACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGSNGVA+LEGYQSDLAY
Sbjct: 121 DKFVQTEGGFACALDLVKHIRGKYGDYFGITVAGYPEAHPDVIGSNGVASLEGYQSDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LKRK DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 181 LKRKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P EI AALEPIKDNEEAVKAYGIHLGTEMCKKILAHGI+TLHLYTLNMEKSALAILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIRTLHLYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEESKI R LPWRRPTN+FRV EDVRPIFWANRPKSY+SRTIGWDQYPHGRW DSR
Sbjct: 301 NLGLIEESKIQRPLPWRRPTNVFRVKEDVRPIFWANRPKSYLSRTIGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGAL+DYQFMRPRAR KKL+EEW  PL +I+DVYEKF K+CLGKLRSSPWSELDGLQ
Sbjct: 361 NPSYGALSDYQFMRPRARGKKLIEEWVTPLTNIDDVYEKFKKFCLGKLRSSPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ETRII++QLG+IN KG LTINSQP+VNGERSDSP VGWGG GGYVYQKAY+EFFCS+EK
Sbjct: 421 PETRIINDQLGRINTKGFLTINSQPAVNGERSDSPVVGWGGPGGYVYQKAYLEFFCSREK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+VDKCRALPSLTY+AVNKEG W+SNI Q  VNAVTWGVFPA+EIIQPTVVDPASF V
Sbjct: 481 LDALVDKCRALPSLTYVAVNKEGSWLSNIGQSQVNAVTWGVFPAREIIQPTVVDPASFMV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWA  YPEGD SRKLL+EI+ +Y+LVSLVDNDY+N ++FA   DF
Sbjct: 541 WKDEAFEIWSRGWARHYPEGDASRKLLQEIENAYFLVSLVDNDYINGDLFAVFADF 595

BLAST of CmaCh04G009640 vs. NCBI nr
Match: gi|1021576668|ref|XP_016179517.1| (PREDICTED: probable methylenetetrahydrofolate reductase [Arachis ipaensis])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 512/596 (85.91%), Postives = 555/596 (93.12%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDARVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KIRAA   D  RVVFSFE+FPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIEKIRAAAAADPDRVVFSFEFFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLALRGDPPHGQ 120
           TADLTL+IANKMQNM+CVETMMHLTCTNMPVEKIDHALQTIKSNG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANKMQNMVCVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATLEGYQSDLAY 180
           DKFVQIEGGFACA DLVKHIR KYGDYFGITVAGYPEAHPDVIGS+G+AT EGYQ+DLAY
Sbjct: 121 DKFVQIEGGFACARDLVKHIRAKYGDYFGITVAGYPEAHPDVIGSDGLATPEGYQNDLAY 180

Query: 181 LKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKGFIRMTEFCK 240
           LK K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPINNYKGF+RMT FCK
Sbjct: 181 LKSKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNMEKSALAILM 300
           TK+P +I AALEPIKDNEEAVK+YGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPADIFAALEPIKDNEEAVKSYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 SLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQYPHGRWTDSR 360
           +LGLIEE+KI+RSLPWRRP N+FRV EDVRPIFWANRPKSYISRTIGWDQYPHGRW DSR
Sbjct: 301 NLGLIEETKINRSLPWRRPANVFRVREDVRPIFWANRPKSYISRTIGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420
           NPSYGALTDYQFMRPRARDKKL+EEW +PL SIED+YE+F +YCLGKLRS+PWSELDGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLVEEWVVPLRSIEDIYERFKQYCLGKLRSNPWSELDGLQ 420

Query: 421 AETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSKEK 480
            ET+II+EQL KIN+KG LTINSQP+VNGE+SDS +VGWGG GGYVYQKAY+EFFCSKEK
Sbjct: 421 PETKIINEQLEKINLKGFLTINSQPAVNGEKSDSATVGWGGPGGYVYQKAYVEFFCSKEK 480

Query: 481 LDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540
           LDA+V+KC+   SLT+MAVNKEG W SN+ Q DVNAVTWGVFPAKEIIQPT+VDP SF V
Sbjct: 481 LDALVEKCKNRSSLTFMAVNKEGSWRSNVGQTDVNAVTWGVFPAKEIIQPTIVDPVSFMV 540

Query: 541 WKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFAALEDF 597
           WKDEAFE+WSRGWA LYPEGD SRKL+EE+  SY+LVSLVDNDYVN ++FA   DF
Sbjct: 541 WKDEAFEIWSRGWACLYPEGDASRKLVEEVGNSYFLVSLVDNDYVNGDLFAVFADF 596

BLAST of CmaCh04G009640 vs. NCBI nr
Match: gi|743920960|ref|XP_011004536.1| (PREDICTED: methylenetetrahydrofolate reductase 2 [Populus euphratica])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 510/608 (83.88%), Postives = 554/608 (91.12%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDA-------------RVVFSFEYFPPKTEDGVDNLFERMDRMVAHN 60
           MKVIDKIRAA    D+             +VVFSFE+FPPKT+DGVDNLFERMDRMV+HN
Sbjct: 1   MKVIDKIRAAAAAGDSNANGNGGDSCSNNKVVFSFEFFPPKTDDGVDNLFERMDRMVSHN 60

Query: 61  PSFCDITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQ 120
           PSFCDITWGAGGSTADLTLDIANKMQN+ICVETMMHLTCTNMPVEKIDHAL+TIKSNGIQ
Sbjct: 61  PSFCDITWGAGGSTADLTLDIANKMQNIICVETMMHLTCTNMPVEKIDHALETIKSNGIQ 120

Query: 121 NVLALRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNG 180
           NVLALRGDPPHGQDKFVQI+GG ACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGS+G
Sbjct: 121 NVLALRGDPPHGQDKFVQIQGGLACALDLVKHIRSKYGDYFGITVAGYPEAHPDVIGSDG 180

Query: 181 VATLEGYQSDLAYLKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPIN 240
            ATLE Y  DLAYLK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPI+PGIMPIN
Sbjct: 181 FATLEDYHKDLAYLKQKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPIN 240

Query: 241 NYKGFIRMTEFCKTKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLY 300
           NYKGFIRMT FCKTKVP E+ AALEPIKDNEEAV+AYGIHLGTEMCKKILAHGIKTLHLY
Sbjct: 241 NYKGFIRMTGFCKTKVPAEVTAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLY 300

Query: 301 TLNMEKSALAILMSLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIG 360
           TLNMEKSALAILM+LGLIEESKI+R LPWR PTN+FRV EDVRPIFWANRPKSY++RTIG
Sbjct: 301 TLNMEKSALAILMNLGLIEESKITRPLPWRPPTNVFRVKEDVRPIFWANRPKSYLTRTIG 360

Query: 361 WDQYPHGRWTDSRNPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGK 420
           WDQYPHGRW DSRNPSYGAL+DYQFMRPRAR KKLLEEWA PL S+ED+YEKF  YCLGK
Sbjct: 361 WDQYPHGRWGDSRNPSYGALSDYQFMRPRARGKKLLEEWAAPLKSVEDIYEKFKVYCLGK 420

Query: 421 LRSSPWSELDGLQAETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVY 480
           L+SSPWSELDGLQ ET+II+EQLGKIN+KG LTINSQP+VNGE+SDSPSVGWGG GGYVY
Sbjct: 421 LKSSPWSELDGLQPETKIINEQLGKINLKGFLTINSQPAVNGEKSDSPSVGWGGPGGYVY 480

Query: 481 QKAYIEFFCSKEKLDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEI 540
           QKAY+EFFCSK+KL+A+VDKC++ P +TY+AVNK G W+SN+   DVNAVTWGVFPAKEI
Sbjct: 481 QKAYLEFFCSKDKLNALVDKCKSFPFVTYIAVNKGGSWISNVALTDVNAVTWGVFPAKEI 540

Query: 541 IQPTVVDPASFHVWKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNS 596
           IQPTVV+P SF VWKDEAFE+WSRGWASLYPEGDPSR LLEE+Q SY+LVSLVDNDY++ 
Sbjct: 541 IQPTVVEPTSFSVWKDEAFEIWSRGWASLYPEGDPSRTLLEEVQSSYFLVSLVDNDYIHG 600

BLAST of CmaCh04G009640 vs. NCBI nr
Match: gi|566210462|ref|XP_006372317.1| (Methylenetetrahydrofolate reductase family protein [Populus trichocarpa])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 507/604 (83.94%), Postives = 551/604 (91.23%), Query Frame = 1

Query: 1   MKVIDKIRAATTGDDA---------RVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFC 60
           MKVIDKIRAA    D+         +VVFSFE+FPPKT+DGVDNLFERMDRMV+HNPSFC
Sbjct: 1   MKVIDKIRAAAAAGDSNGGDSSSNNKVVFSFEFFPPKTDDGVDNLFERMDRMVSHNPSFC 60

Query: 61  DITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGIQNVLA 120
           DITWGAGGSTADLTLDIANKMQN+ICVETMMHLTCTNMPVEKIDHAL+TIKSNGIQNVLA
Sbjct: 61  DITWGAGGSTADLTLDIANKMQNIICVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLA 120

Query: 121 LRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATL 180
           LRGDPPHGQDKFVQI+GG ACALDLVKHIR KYGDYFGITVAGYPEAHPDVIGS+G ATL
Sbjct: 121 LRGDPPHGQDKFVQIQGGLACALDLVKHIRSKYGDYFGITVAGYPEAHPDVIGSDGFATL 180

Query: 181 EGYQSDLAYLKRKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIIPGIMPINNYKG 240
           E Y  DLAYLK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPI+PGIMPINNYKG
Sbjct: 181 EDYHKDLAYLKQKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPINNYKG 240

Query: 241 FIRMTEFCKTKVPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHLYTLNM 300
           FIRM  FCKTK+P E+ AALEPIKDNEEAV+AYGIHLGTEMCKKILAHGIKTLHLYTLNM
Sbjct: 241 FIRMAGFCKTKIPAEVTAALEPIKDNEEAVRAYGIHLGTEMCKKILAHGIKTLHLYTLNM 300

Query: 301 EKSALAILMSLGLIEESKISRSLPWRRPTNIFRVHEDVRPIFWANRPKSYISRTIGWDQY 360
           EKSALAILM+LGLIEESKI+R LPWR PTN+FRV EDVRPIFWANRPKSY++RTIGWDQY
Sbjct: 301 EKSALAILMNLGLIEESKITRPLPWRPPTNVFRVKEDVRPIFWANRPKSYLTRTIGWDQY 360

Query: 361 PHGRWTDSRNPSYGALTDYQFMRPRARDKKLLEEWALPLNSIEDVYEKFMKYCLGKLRSS 420
           PHGRW DSRNPSYGAL+DYQFMRPRAR KKLLEEWA PL S+ED+YEKF  YCLGKL+SS
Sbjct: 361 PHGRWGDSRNPSYGALSDYQFMRPRARGKKLLEEWAAPLKSVEDIYEKFKVYCLGKLKSS 420

Query: 421 PWSELDGLQAETRIISEQLGKINMKGLLTINSQPSVNGERSDSPSVGWGGSGGYVYQKAY 480
           PWSELDGLQ ET+II+EQLGKIN+KG LTINSQP+VNGE+SDSPSVGWGG GGYVYQKAY
Sbjct: 421 PWSELDGLQPETKIINEQLGKINLKGFLTINSQPAVNGEKSDSPSVGWGGPGGYVYQKAY 480

Query: 481 IEFFCSKEKLDAVVDKCRALPSLTYMAVNKEGVWVSNITQKDVNAVTWGVFPAKEIIQPT 540
           +EFFCSK+KL+A+VDKC++ P +TY+AVNK G W+SN+   DVNAVTWGVFP KEIIQPT
Sbjct: 481 LEFFCSKDKLNALVDKCKSFPFVTYIAVNKGGSWISNVALTDVNAVTWGVFPGKEIIQPT 540

Query: 541 VVDPASFHVWKDEAFELWSRGWASLYPEGDPSRKLLEEIQISYYLVSLVDNDYVNSNIFA 596
           VVDP SF VWKDEAFE+WSRGWASLYPEGDPSR LLEE+Q SY+LVSLVDNDY++ +IFA
Sbjct: 541 VVDPTSFSVWKDEAFEIWSRGWASLYPEGDPSRTLLEEVQSSYFLVSLVDNDYIHGDIFA 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MTHR2_ARATH9.8e-30381.01Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana GN=MTHFR2 PE=1 SV=... [more]
MTHR_ORYSJ8.6e-29981.01Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica GN=... [more]
MTHR1_MAIZE5.2e-29679.80Methylenetetrahydrofolate reductase 1 OS=Zea mays PE=2 SV=1[more]
MTHR1_ARATH6.0e-29277.66Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana GN=MTHFR1 PE=1 SV=... [more]
MTHR_HUMAN6.6e-15045.10Methylenetetrahydrofolate reductase OS=Homo sapiens GN=MTHFR PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0LIC6_CUCSA0.0e+0093.29Methylenetetrahydrofolate reductase OS=Cucumis sativus GN=Csa_2G149470 PE=3 SV=1[more]
U5FIP4_POPTR0.0e+0083.94Methylenetetrahydrofolate reductase OS=Populus trichocarpa GN=POPTR_0017s00520g ... [more]
A0A0B2SRC1_GLYSO0.0e+0084.73Methylenetetrahydrofolate reductase OS=Glycine soja GN=glysoja_018451 PE=3 SV=1[more]
I1JZE2_SOYBN0.0e+0084.73Methylenetetrahydrofolate reductase OS=Glycine max GN=GLYMA_04G256100 PE=3 SV=1[more]
A0A072VS77_MEDTR0.0e+0084.56Methylenetetrahydrofolate reductase OS=Medicago truncatula GN=MTR_1g040105 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G44160.15.5e-30481.01 methylenetetrahydrofolate reductase 2[more]
AT3G59970.33.4e-29377.66 methylenetetrahydrofolate reductase 1[more]
Match NameE-valueIdentityDescription
gi|778668497|ref|XP_004138970.2|0.0e+0093.29PREDICTED: methylenetetrahydrofolate reductase 2 [Cucumis sativus][more]
gi|1009142164|ref|XP_015888576.1|0.0e+0086.41PREDICTED: methylenetetrahydrofolate reductase 2-like [Ziziphus jujuba][more]
gi|1021576668|ref|XP_016179517.1|0.0e+0085.91PREDICTED: probable methylenetetrahydrofolate reductase [Arachis ipaensis][more]
gi|743920960|ref|XP_011004536.1|0.0e+0083.88PREDICTED: methylenetetrahydrofolate reductase 2 [Populus euphratica][more]
gi|566210462|ref|XP_006372317.1|0.0e+0083.94Methylenetetrahydrofolate reductase family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003171Mehydrof_redctse
IPR004621Fadh2_euk
Vocabulary: Molecular Function
TermDefinition
GO:0004489methylenetetrahydrofolate reductase (NAD(P)H) activity
Vocabulary: Biological Process
TermDefinition
GO:0006555methionine metabolic process
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0035999 tetrahydrofolate interconversion
biological_process GO:0006555 methionine metabolic process
biological_process GO:0046653 tetrahydrofolate metabolic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005575 cellular_component
molecular_function GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G009640.1CmaCh04G009640.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003171Methylenetetrahydrofolate reductasePFAMPF02219MTHFRcoord: 15..303
score: 9.5E
IPR004621Eukaryotic-type methylenetetrahydrofolate reductaseTIGRFAMsTIGR00677TIGR00677coord: 19..307
score: 1.7E
NoneNo IPR availablePANTHERPTHR21091METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATEDcoord: 131..149
score: 1.4E-242coord: 180..595
score: 1.4E
NoneNo IPR availablePANTHERPTHR21091:SF31METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 131..149
score: 1.4E-242coord: 180..595
score: 1.4E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh04G009640CmoCh15G015100Cucurbita moschata (Rifu)cmacmoB699
CmaCh04G009640CmoCh04G010400Cucurbita moschata (Rifu)cmacmoB728
CmaCh04G009640Cp4.1LG01g08000Cucurbita pepo (Zucchini)cmacpeB720
CmaCh04G009640Carg00080Silver-seed gourdcarcmaB0948
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh04G009640CmaCh15G014320Cucurbita maxima (Rimu)cmacmaB331
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh04G009640Watermelon (97103) v2cmawmbB780
CmaCh04G009640Cucurbita moschata (Rifu)cmacmoB700