CmaCh04G008360 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh04G008360
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Probable tRNA N6-adenosine threonylcarbamoyltransferase
Location	Cma_Chr04 : 4300813 .. 4301459 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAAATGATAGCACTGGGTTTTGAGGGTTCAGCAAACAAGATTGGTGTTGGGGTTGTGACATTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCCGGCCATGGCTTTCTTCCTCGTGAAACTGCACATCACCATTTTGAGCACATTCTCCCGTTGATTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCTCTACAAGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGCGTTGCACATATTGAGATGGGAAGGATTGTAACGGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCCGTTGGGAATTGCTTGGATCGGTTTGCAAGAGTCCTAACGCTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGTGTGTTATTGGAAGATGATAATGTAAATTTTTCGTTATTCCATTTATTCTATTCATTGGATTATTGGGATTGATAGGGCATCGAAGCTTAA

mRNA sequence

ATGAAGAAAATGATAGCACTGGGTTTTGAGGGTTCAGCAAACAAGATTGGTGTTGGGGTTGTGACATTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCCGGCCATGGCTTTCTTCCTCGTGAAACTGCACATCACCATTTTGAGCACATTCTCCCGTTGATTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCTCTACAAGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGCGTTGCACATATTGAGATGGGAAGGATTGTAACGGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCCGTTGGGAATTGCTTGGATCGGTTTGCAAGAGTCCTAACGCTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGGCATCGAAGCTTAA

Coding sequence (CDS)

ATGAAGAAAATGATAGCACTGGGTTTTGAGGGTTCAGCAAACAAGATTGGTGTTGGGGTTGTGACATTAGATGGCAACATTTTGTCAAACCCACGTCACACCTACATTACACCTCCCGGCCATGGCTTTCTTCCTCGTGAAACTGCACATCACCATTTTGAGCACATTCTCCCGTTGATTAAGTCTGCTCTGGAAACTGCAAAAATTACCCCAGAGGATATTGACTGCCTCTGTTACACTAAAGGGCCTGGCATGGGAGCTCCTCTACAAGTTTCTGCTGTTGCTGTGAGGGTTCTTTCTCAGATTTGGAATAAGCCTATTGTTGCTGTTAATCATTGCGTTGCACATATTGAGATGGGAAGGATTGTAACGGGTGCTGATGATCCTGTTGTCTTGTATGTTAGTGGTGGGAATACTCAGGTGATTGCTTATAGTGAAGGAAGGTATAGAATCTTTGGGGAAACTATTGATATTGCCGTTGGGAATTGCTTGGATCGGTTTGCAAGAGTCCTAACGCTATCCAATGACCCAAGTCCTGGATACAATATTGAACAGGGCATCGAAGCTTAA

Protein sequence

MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQGIEA

Homology

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BLAST of CmaCh04G008360 vs. Swiss-Prot
Match: OSGEP_NEMVE (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensis GN=osgep PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 1.8e-77
Identity = 129/179 (72.07%), Postives = 155/179 (86.59%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSALET 66
           +GFEGSANK+G+G++  DG +LSNPRHTYITPPG GF+PR+TA HH EH + +++ AL+ 
Sbjct: 5   IGFEGSANKLGIGIIR-DGVVLSNPRHTYITPPGQGFMPRDTAKHHQEHAIDILRRALDE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGA 126
           A+I P+DIDC+CYTKGPGMGAPL   AV  R ++Q+W KPI+ VNHC+ HIEMGR++TGA
Sbjct: 65  AQIRPQDIDCICYTKGPGMGAPLVAVAVVARTVAQLWKKPIIGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQ 186
           ++P VLYVSGGNTQVIAY + RYRIFGETIDIAVGNCLDRFARVL LSNDPSPGYNIEQ
Sbjct: 125 NNPTVLYVSGGNTQVIAYLQKRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGYNIEQ 182

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BLAST of CmaCh04G008360 vs. Swiss-Prot
Match: OSGEP_DICDI (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Dictyostelium discoideum GN=osgep PE=3 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 1.3e-75
Identity = 129/179 (72.07%), Postives = 154/179 (86.03%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSALET 66
           +GFEGSANK+G+G+V  DG ILSN RHT+ITPPG GFLP++TA HH   IL L++ +LE 
Sbjct: 5   MGFEGSANKLGIGIVKDDGTILSNIRHTFITPPGEGFLPKDTAKHHRSFILSLVEKSLEE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGA 126
           +K+ P DIDCL YTKGPGMG PL+  AV VR+LSQ+W++PIVAVNHC+AHIEMGR++TGA
Sbjct: 65  SKLKPSDIDCLAYTKGPGMGPPLRSVAVTVRMLSQLWDRPIVAVNHCIAHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQ 186
            DP +LYVSGGNTQVI+YS  +YRIFGETIDIAVGNCLDRFARV+ + NDPSPGYNIEQ
Sbjct: 125 VDPTILYVSGGNTQVISYSLKKYRIFGETIDIAVGNCLDRFARVIQIPNDPSPGYNIEQ 183

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BLAST of CmaCh04G008360 vs. Swiss-Prot
Match: OSGEP_BOVIN (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus GN=OSGEP PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 2.5e-74
Identity = 132/179 (73.74%), Postives = 150/179 (83.80%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGA 126
           A +T EDIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP++ VNHC+ HIEMGR++TGA
Sbjct: 65  AGLTSEDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLLGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQ 186
            +P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ
Sbjct: 125 TNPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQ 182

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BLAST of CmaCh04G008360 vs. Swiss-Prot
Match: OSGEP_XENLA (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Xenopus laevis GN=osgep PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 5.5e-74
Identity = 127/181 (70.17%), Postives = 151/181 (83.43%), Query Frame = 1


Query: 5   IALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSAL 64
           I +GFEGSANKIGVG++  DG +LSNPR TYITPPG GF+P +TA HH   IL +++ AL
Sbjct: 3   IVVGFEGSANKIGVGIIQ-DGKVLSNPRRTYITPPGQGFMPSDTARHHRSCILDVLQEAL 62

Query: 65  ETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVT 124
           E + I PED+DC+ YTKGPGMGAPL   A+  R ++Q+W KP++ VNHC+ HIEMGR++T
Sbjct: 63  EESNIKPEDVDCVAYTKGPGMGAPLLSVAIVARTVAQLWKKPLLGVNHCIGHIEMGRLIT 122

Query: 125 GADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIE 184
           GA++P VLYVSGGNTQVIAYSE  YRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIE
Sbjct: 123 GAENPTVLYVSGGNTQVIAYSERCYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIE 182

Query: 185 Q 186
           Q
Sbjct: 183 Q 182

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BLAST of CmaCh04G008360 vs. Swiss-Prot
Match: OSGEP_HUMAN (Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens GN=OSGEP PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 7.2e-74
Identity = 131/179 (73.18%), Postives = 149/179 (83.24%), Query Frame = 1


Query: 7   LGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIKSALET 66
           LGFEGSANKIGVGVV  DG +L+NPR TY+TPPG GFLP +TA HH   IL L++ AL  
Sbjct: 5   LGFEGSANKIGVGVVR-DGKVLANPRRTYVTPPGTGFLPGDTARHHRAVILDLLQEALTE 64

Query: 67  AKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGRIVTGA 126
           + +T +DIDC+ YTKGPGMGAPL   AV  R ++Q+WNKP+V VNHC+ HIEMGR++TGA
Sbjct: 65  SGLTSQDIDCIAYTKGPGMGAPLVSVAVVARTVAQLWNKPLVGVNHCIGHIEMGRLITGA 124

Query: 127 DDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGYNIEQ 186
             P VLYVSGGNTQVIAYSE RYRIFGETIDIAVGNCLDRFARVL +SNDPSPGYNIEQ
Sbjct: 125 TSPTVLYVSGGNTQVIAYSEHRYRIFGETIDIAVGNCLDRFARVLKISNDPSPGYNIEQ 182

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BLAST of CmaCh04G008360 vs. TrEMBL
Match: A0A0A0KR08_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517870 PE=4 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 5.3e-100
Identity = 178/185 (96.22%), Postives = 181/185 (97.84%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKM ALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETA HH  HILPL+
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

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BLAST of CmaCh04G008360 vs. TrEMBL
Match: W9R4Z4_9ROSA (Putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep OS=Morus notabilis GN=L484_024755 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 2.5e-97
Identity = 173/185 (93.51%), Postives = 179/185 (96.76%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKMIALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETA HH +H+LPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITPE+IDCLCYTKGPGMGAPLQVSAV VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPEEIDCLCYTKGPGMGAPLQVSAVVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

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BLAST of CmaCh04G008360 vs. TrEMBL
Match: M5XC29_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007957mg PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.2e-96
Identity = 172/185 (92.97%), Postives = 179/185 (96.76%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKMIALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETA HH +HILPLI
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITP++IDCLCYTKGPGMGAPLQV+A+ VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPKEIDCLCYTKGPGMGAPLQVAAIVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

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BLAST of CmaCh04G008360 vs. TrEMBL
Match: D7ME14_ARALL (Glycoprotease M22 family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_914341 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 1.6e-96
Identity = 166/184 (90.22%), Postives = 179/184 (97.28%), Query Frame = 1


Query: 2   KKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIK 61
           KKMIA+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETAHHH +H+LPL+K
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 121
           SALET+++TPE+IDCLCYTKGPGMGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCLCYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 IVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           +VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQ 186
           NIEQ
Sbjct: 183 NIEQ 186

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BLAST of CmaCh04G008360 vs. TrEMBL
Match: O49653_ARATH (Actin-like ATPase superfamily protein OS=Arabidopsis thaliana GN=T12H17.110 PE=2 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 2.7e-96
Identity = 165/184 (89.67%), Postives = 179/184 (97.28%), Query Frame = 1


Query: 2   KKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIK 61
           KKMIA+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETAHHH +H+LPL+K
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 121
           SALET+++TPE+IDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 IVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           +VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQ 186
           NIEQ
Sbjct: 183 NIEQ 186

[Close]

BLAST of CmaCh04G008360 vs. TAIR10
Match: AT4G22720.1 (AT4G22720.1 Actin-like ATPase superfamily protein)

HSP 1 Score: 359.4 bits (921), Expect = 1.4e-99
Identity = 165/184 (89.67%), Postives = 179/184 (97.28%), Query Frame = 1


Query: 2   KKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLIK 61
           KKMIA+GFEGSANKIGVG+VTLDG IL+NPRHTYITPPGHGFLPRETAHHH +H+LPL+K
Sbjct: 3   KKMIAIGFEGSANKIGVGIVTLDGTILANPRHTYITPPGHGFLPRETAHHHLDHVLPLVK 62

Query: 62  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 121
           SALET+++TPE+IDC+CYTKGPGMGAPLQVSA+ VRVLSQ+W KPIVAVNHCVAHIEMGR
Sbjct: 63  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 122

Query: 122 IVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPGY 181
           +VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVL LSNDPSPGY
Sbjct: 123 VVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPSPGY 182

Query: 182 NIEQ 186
           NIEQ
Sbjct: 183 NIEQ 186

[Close]

BLAST of CmaCh04G008360 vs. TAIR10
Match: AT2G45270.1 (AT2G45270.1 glycoprotease 1)

HSP 1 Score: 82.8 bits (203), Expect = 2.5e-16
Identity = 56/187 (29.95%), Postives = 92/187 (49.20%), Query Frame = 1


Query: 4   MIALGFEGSANKIGVGVVTLDGNILSN--PRHTYITPPGHGFLPRETAHHHFEHILPLIK 63
           ++ LG E S +     VV  +G ILS        +     G  P++    H   I  +++
Sbjct: 84  LVVLGIETSCDDTAAAVVRGNGEILSQVISSQAELLVQYGGVAPKQAEEAHSRVIDKVVQ 143

Query: 64  SALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMGR 123
            AL+ A +T +D+  +  T GPG+   L+V     R ++  ++ PIV V+H  AH  + R
Sbjct: 144 DALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNFSLPIVGVHHMEAHALVAR 203

Query: 124 IVTG--ADDPVVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPS 183
           +V    +   + L +SGG N  V+A+  G+Y   G T+D A+G   D+ A+ L L    S
Sbjct: 204 LVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAIGEAFDKTAKWLGLDMHRS 263

Query: 184 PGYNIEQ 186
            G  +E+
Sbjct: 264 GGPAVEE 270

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BLAST of CmaCh04G008360 vs. NCBI nr
Match: gi|449450050|ref|XP_004142777.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 7.6e-100
Identity = 178/185 (96.22%), Postives = 181/185 (97.84%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKM ALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETA HH  HILPL+
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

[Close]

BLAST of CmaCh04G008360 vs. NCBI nr
Match: gi|700196162|gb|KGN51339.1| (hypothetical protein Csa_5G517870 [Cucumis sativus])

HSP 1 Score: 371.7 bits (953), Expect = 7.6e-100
Identity = 178/185 (96.22%), Postives = 181/185 (97.84%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKM ALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETA HH  HILPL+
Sbjct: 1   MKKMTALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAQHHLHHILPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAKITP+DIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAKITPKDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           R+VTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RVVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

[Close]

BLAST of CmaCh04G008360 vs. NCBI nr
Match: gi|703102734|ref|XP_010097542.1| (putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep [Morus notabilis])

HSP 1 Score: 362.8 bits (930), Expect = 3.5e-97
Identity = 173/185 (93.51%), Postives = 179/185 (96.76%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKMIALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETA HH +H+LPL+
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITPE+IDCLCYTKGPGMGAPLQVSAV VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPEEIDCLCYTKGPGMGAPLQVSAVVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

[Close]

BLAST of CmaCh04G008360 vs. NCBI nr
Match: gi|951071892|ref|XP_014491422.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Vigna radiata var. radiata])

HSP 1 Score: 361.3 bits (926), Expect = 1.0e-96
Identity = 170/185 (91.89%), Postives = 178/185 (96.22%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MK+MIALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETA HH +H+LPL+
Sbjct: 1   MKRMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHVLPLV 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETAK+TP+DIDCLCYTKGPGMGAPLQVSA+ VRVLSQ+W KPIV VNHCVAHIEMG
Sbjct: 61  KSALETAKVTPQDIDCLCYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVGVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

[Close]

BLAST of CmaCh04G008360 vs. NCBI nr
Match: gi|645231617|ref|XP_008222479.1| (PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Prunus mume])

HSP 1 Score: 360.5 bits (924), Expect = 1.8e-96
Identity = 172/185 (92.97%), Postives = 179/185 (96.76%), Query Frame = 1


Query: 1   MKKMIALGFEGSANKIGVGVVTLDGNILSNPRHTYITPPGHGFLPRETAHHHFEHILPLI 60
           MKKMIALGFEGSANKIGVGVVTLDG ILSNPRHTYITPPG GFLPRETA HH +HILPLI
Sbjct: 1   MKKMIALGFEGSANKIGVGVVTLDGTILSNPRHTYITPPGQGFLPRETAQHHLQHILPLI 60

Query: 61  KSALETAKITPEDIDCLCYTKGPGMGAPLQVSAVAVRVLSQIWNKPIVAVNHCVAHIEMG 120
           KSALETA+ITP++IDCLCYTKGPGMGAPLQV+A+ VRVLSQ+W KPIVAVNHCVAHIEMG
Sbjct: 61  KSALETAQITPKEIDCLCYTKGPGMGAPLQVAAIVVRVLSQLWKKPIVAVNHCVAHIEMG 120

Query: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180
           RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG
Sbjct: 121 RIVTGADDPVVLYVSGGNTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLTLSNDPSPG 180

Query: 181 YNIEQ 186
           YNIEQ
Sbjct: 181 YNIEQ 185

The following BLAST results are available for this feature:

BLAST of CmaCh04G008360 vs. Swiss-Prot


Match Name	E-value	Identity	Description
OSGEP_NEMVE	1.8e-77	72.07	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Nematostella vectensi...	[more]
OSGEP_DICDI	1.3e-75	72.07	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Dictyostelium discoid...	[more]
OSGEP_BOVIN	2.5e-74	73.74	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Bos taurus GN=OSGEP P...	[more]
OSGEP_XENLA	5.5e-74	70.17	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Xenopus laevis GN=osg...	[more]
OSGEP_HUMAN	7.2e-74	73.18	Probable tRNA N6-adenosine threonylcarbamoyltransferase OS=Homo sapiens GN=OSGEP...	[more]

BLAST of CmaCh04G008360 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KR08_CUCSA	5.3e-100	96.22	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G517870 PE=4 SV=1	[more]
W9R4Z4_9ROSA	2.5e-97	93.51	Putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep OS=Morus not...	[more]
M5XC29_PRUPE	1.2e-96	92.97	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007957mg PE=4 SV=1	[more]
D7ME14_ARALL	1.6e-96	90.22	Glycoprotease M22 family protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA...	[more]
O49653_ARATH	2.7e-96	89.67	Actin-like ATPase superfamily protein OS=Arabidopsis thaliana GN=T12H17.110 PE=2...	[more]

BLAST of CmaCh04G008360 vs. TAIR10


Match Name	E-value	Identity	Description
AT4G22720.1	1.4e-99	89.67	Actin-like ATPase superfamily protein	[more]
AT2G45270.1	2.5e-16	29.95	glycoprotease 1	[more]

BLAST of CmaCh04G008360 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449450050\|ref\|XP_004142777.1\|	7.6e-100	96.22	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Cucumis sati...	[more]
gi\|700196162\|gb\|KGN51339.1\|	7.6e-100	96.22	hypothetical protein Csa_5G517870 [Cucumis sativus]	[more]
gi\|703102734\|ref\|XP_010097542.1\|	3.5e-97	93.51	putative tRNA threonylcarbamoyladenosine biosynthesis protein osgep [Morus notab...	[more]
gi\|951071892\|ref\|XP_014491422.1\|	1.0e-96	91.89	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Vigna radiat...	[more]
gi\|645231617\|ref\|XP_008222479.1\|	1.8e-96	92.97	PREDICTED: probable tRNA N6-adenosine threonylcarbamoyltransferase [Prunus mume]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000905	Gcp-like_dom
IPR017861	KAE1/TsaD

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030048	actin filament-based movement
biological_process	GO:0051645	Golgi localization
biological_process	GO:0051646	mitochondrion localization
biological_process	GO:0060151	peroxisome localization
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0006508	proteolysis
biological_process	GO:0002949	tRNA threonylcarbamoyladenosine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0000408	EKC/KEOPS complex
molecular_function	GO:0008233	peptidase activity
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G008360.1	CmaCh04G008360.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000905	Gcp-like domain	PFAM	PF00814	Peptidase_M22	coord: 28..185 score: 1.4
IPR017861	Kae1/TsaD family	PRINTS	PR00789	OSIALOPTASE	coord: 130..142 score: 4.7E-34coord: 7..20 score: 4.7E-34coord: 152..173 score: 4.7E-34coord: 77..97 score: 4.7E-34coord: 98..117 score: 4.7
None	No IPR available	GENE3D	G3DSA:3.30.420.40		coord: 4..157 score: 1.3
None	No IPR available	PANTHER	PTHR11735	O-SIALOGLYCOPROTEIN ENDOPEPTIDASE	coord: 1..185 score: 1.9E
None	No IPR available	PANTHER	PTHR11735:SF14	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE-RELATED	coord: 1..185 score: 1.9E
None	No IPR available	unknown	SSF53067	Actin-like ATPase domain	coord: 6..124 score: 1.94E-25coord: 103..171 score: 1.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh04G008360	Cla004856	Watermelon (97103) v1	cmawmB654
CmaCh04G008360	ClCG10G008650	Watermelon (Charleston Gray)	cmawcgB614
CmaCh04G008360	CmoCh04G008940	Cucurbita moschata (Rifu)	cmacmoB728
CmaCh04G008360	Lsi05G008300	Bottle gourd (USVL1VR-Ls)	cmalsiB692
CmaCh04G008360	Cp4.1LG01g09040	Cucurbita pepo (Zucchini)	cmacpeB720
CmaCh04G008360	Cla97C10G192000	Watermelon (97103) v2	cmawmbB718
CmaCh04G008360	Bhi11G000698	Wax gourd	cmawgoB0815
CmaCh04G008360	Carg14237	Silver-seed gourd	carcmaB1288

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh04G008360	Cucurbita moschata (Rifu)	cmacmoB700
CmaCh04G008360	Cucurbita moschata (Rifu)	cmacmoB727
CmaCh04G008360	Wild cucumber (PI 183967)	cmacpiB717
CmaCh04G008360	Wild cucumber (PI 183967)	cmacpiB785
CmaCh04G008360	Cucumber (Chinese Long) v2	cmacuB711
CmaCh04G008360	Melon (DHL92) v3.5.1	cmameB698
CmaCh04G008360	Melon (DHL92) v3.5.1	cmameB710
CmaCh04G008360	Watermelon (Charleston Gray)	cmawcgB639
CmaCh04G008360	Watermelon (97103) v1	cmawmB715
CmaCh04G008360	Cucurbita pepo (Zucchini)	cmacpeB714
CmaCh04G008360	Cucurbita pepo (Zucchini)	cmacpeB702
CmaCh04G008360	Cucurbita pepo (Zucchini)	cmacpeB741
CmaCh04G008360	Bottle gourd (USVL1VR-Ls)	cmalsiB639
CmaCh04G008360	Cucumber (Gy14) v2	cgybcmaB544
CmaCh04G008360	Melon (DHL92) v3.6.1	cmamedB788
CmaCh04G008360	Melon (DHL92) v3.6.1	cmamedB804
CmaCh04G008360	Silver-seed gourd	carcmaB1414
CmaCh04G008360	Cucumber (Chinese Long) v3	cmacucB0917
CmaCh04G008360	Cucumber (Chinese Long) v3	cmacucB0845
CmaCh04G008360	Watermelon (97103) v2	cmawmbB712
CmaCh04G008360	Wax gourd	cmawgoB0822
CmaCh04G008360	Wax gourd	cmawgoB0883
CmaCh04G008360	Cucurbita maxima (Rimu)	cmacmaB350
CmaCh04G008360	Cucurbita maxima (Rimu)	cmacmaB546