CmaCh04G005210 (gene) Cucurbita maxima (Rimu)

NameCmaCh04G005210
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
Description6-phosphogluconate dehydrogenase family protein
LocationCma_Chr04 : 2639961 .. 2641070 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTGTGGGGCGAACTTGTGAAGTAAAGATGGATCGCTATGTCTGCTAATCATCTGTAAGTTGGTTTTGAAAATTCCTTATTCCACCATGGAAACTCCTTACCCAATCTCCATCGTTCCCACGAAAACTCGCATTGGATGGATCGGCATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGCGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTGGAGAAGAATGGGCTCCTACAAGGGCTGAATCCTGGTGGGGTCATTGTTGACACCACCAGCAGCCACCCAGATCTTGCCCGCGAGATATTCGCTGCTGCTCGTGCAAAGGGTCGTGTTAATTTCCACTAAATTACTAGATCAAATCGAACCACCTTGAACTAAGAAGTTTAAGCTTATGTACTCGTATTCTTGGCAGGTTGCTGGGCTGTGGATGCGCCCGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGCGAAGCTGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCTGTTGTTCAGAGGCTTAATGGCAAGCAAGTGAATCTGTAG

mRNA sequence

ACTTGTGGGGCGAACTTGTGAAGTAAAGATGGATCGCTATGTCTGCTAATCATCTGTAAGTTGGTTTTGAAAATTCCTTATTCCACCATGGAAACTCCTTACCCAATCTCCATCGTTCCCACGAAAACTCGCATTGGATGGATCGGCATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGCGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTGGAGAAGAATGGGCTCCTACAAGGGCTGAATCCTGGTGGGGTCATTGTTGACACCACCAGCAGCCACCCAGATCTTGCCCGCGAGATATTCGCTGCTGCTCGTGCAAAGGGTTGCTGGGCTGTGGATGCGCCCGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGCGAAGCTGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCTGTTGTTCAGAGGCTTAATGGCAAGCAAGTGAATCTGTAG

Coding sequence (CDS)

ATGGAAACTCCTTACCCAATCTCCATCGTTCCCACGAAAACTCGCATTGGATGGATCGGCATAGGTGTGATGGGCGCCGCCATGGCCACTCGTCTCCTCGCCGCCGGATACTCCGTCGCAATTTACGCTCGTTCACCTTCCAAGGCTCTTGATTTACAGTCAAAAGGCGCTTCTCTCGTTAATTCCGCTTCTGAATTAGCGCAGGTCAGCGATATTGTTTTCACAATGGTGGGTCACCCGTCAGATGTTCGACAAAATGTCTTGGAGAAGAATGGGCTCCTACAAGGGCTGAATCCTGGTGGGGTCATTGTTGACACCACCAGCAGCCACCCAGATCTTGCCCGCGAGATATTCGCTGCTGCTCGTGCAAAGGGTTGCTGGGCTGTGGATGCGCCCGTGTCGGGGGGCGACATTGGCGCTAGAGATGGGAAGTTGGCCATTTTTGGGGGTGGTGATGCAGGGGTAGTGAAGTGGTTGTCACCTCTGTTTGAAGCATTGGGGAAAGTGACATACGTGGGCGAAGCTGGGTGTGGACAGAGCTGCAAGATAGCGAACCAGATAGCGATTGGGGGAAACCTGATGGGGCTGAGTGAAGGGCTGGTGTTCGCTGAGCGGGCGGGGTTGGATGTGAAGCAGTTTGTGGAAGCTGTGAGGGGTGGGGCTGCAGGGTCAATGGCAATGGAGCTGTTTGGTGAGAGGATGATAGAGAGGGACTTTAGGCCTGGTGGGTTTGCTGAGTATATGGTGAAGGATTTGGGAATGGGCGTGGATGTTGTGGAAGAAGGGGAAGATGTGGAGAGGATTGTAGTGTTGCCAGGGGCTGCATTGGCCAAGCAATTGTTCTCTGCAATGGTGGCTAATGGAGATGGAAAGTTTGGCACTCAAGGGCTAATCTCTGTTGTTCAGAGGCTTAATGGCAAGCAAGTGAATCTGTAG

Protein sequence

METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLNGKQVNL
BLAST of CmaCh04G005210 vs. Swiss-Prot
Match: 3HID3_ARATH (Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis thaliana GN=At1g71180 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.9e-103
Identity = 189/306 (61.76%), Postives = 236/306 (77.12%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I P+KTRIGWIGIG+MG+AM + ++AAGYSV +YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELA++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+ +V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQIA   NL+GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM  +             +PGAAL+KQLF+ MVANGDGK G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 322 IRRLNG 316

BLAST of CmaCh04G005210 vs. Swiss-Prot
Match: 3HID2_ARATH (Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis thaliana GN=At1g71170 PE=2 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 9.6e-100
Identity = 182/306 (59.48%), Postives = 229/306 (74.84%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           MET YP  I P+KTRIGWIGIG+MG+AM + +LAAGYSV +YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  EL ++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD+ +V+WL+P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQI +G N++GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM           E  + +PG AL KQLF+ MVANGDGK G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMAA---------EAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 301 IRRLNG 297

BLAST of CmaCh04G005210 vs. Swiss-Prot
Match: 3HID1_ARATH (Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis thaliana GN=At4g29120 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.2e-78
Identity = 151/303 (49.83%), Postives = 219/303 (72.28%), Query Frame = 1

Query: 9   IVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQ 68
           I P+ T+IGWIG GVMG +M   L+ AGY+V ++ R+ SKA  L   GA++ +S + +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  VSDIVFTMVGHPSDVRQNVLE-KNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCW 128
            SD+VFT+VG+PSDVR  +L+ K+G L GL  GGV+VD T+S P LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++GKL+IF GGD   VK L PLF  +GKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEY 248
            I   ++GL EGL++A +AGLDVK+F+EA+  GAAGS +++L+G+R+++RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGEDVERI-VVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLNG 308
            VKDLG+ ++      + +R+ + LPG ALA+QL+ ++ A+G+G  GTQ L+  ++RLN 
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNN 329

Query: 309 KQV 310
             V
Sbjct: 333 VSV 329

BLAST of CmaCh04G005210 vs. Swiss-Prot
Match: YKWC_BACSU (Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) GN=ykwC PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.2e-54
Identity = 109/287 (37.98%), Postives = 183/287 (63.76%), Query Frame = 1

Query: 13  KTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDI 72
           K  IG+IG+GVMG +MA+ +L  G+ V +Y R+  KA  +  KGA   ++  +L++ +D+
Sbjct: 2   KKTIGFIGLGVMGKSMASHILNDGHPVLVYTRTKEKAESILQKGAIWKDTVKDLSKEADV 61

Query: 73  VFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCWAVDAP 132
           + TMVG+PSDV +     NG+++    G  ++D T+S P LA++I  AA+ K  +A+DAP
Sbjct: 62  IITMVGYPSDVEEVYFGSNGIIENAKEGAYLIDMTTSKPSLAKKIAEAAKEKALFALDAP 121

Query: 133 VSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGK-VTYVGEAGCGQSCKIANQIAIGG 192
           VSGGDIGA++G LAI  GG+    +   P+F  +G+ + Y G AG GQ  K+ NQIAI  
Sbjct: 122 VSGGDIGAQNGTLAIMVGGEKEAFEACMPIFSLMGENIQYQGPAGSGQHTKMCNQIAIAA 181

Query: 193 NLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKD 252
            ++G++E + +A+++GL+ +  ++++  GAAGS ++     RM++ +F PG + ++ +KD
Sbjct: 182 GMIGVAEAMAYAQKSGLEPENVLKSITTGAAGSWSLSNLAPRMLQGNFEPGFYVKHFIKD 241

Query: 253 LGMGVDVVE-EGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGL 298
           +G+ ++  E  GE+      +PG +LAK L+  + A G+   GTQ +
Sbjct: 242 MGIALEEAELMGEE------MPGLSLAKSLYDKLAAQGEENSGTQSI 282

BLAST of CmaCh04G005210 vs. Swiss-Prot
Match: GLXR_ECOLI (2-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) GN=glxR PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.1e-42
Identity = 108/289 (37.37%), Postives = 159/289 (55.02%), Query Frame = 1

Query: 15  RIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVF 74
           ++G+IG+G+MG  MA  L  AG+ + +    P  A +L S GA  V +A ++ + SDI+F
Sbjct: 2   KLGFIGLGIMGTPMAINLARAGHQLHVTTIGPV-ADELLSLGAVSVETARQVTEASDIIF 61

Query: 75  TMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCWAVDAPVS 134
            MV     V + +  +NG  +    G  IVD +S  P   +         G   +DAPVS
Sbjct: 62  IMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVS 121

Query: 135 GGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGK-VTYVGEAGCGQSCKIANQIAIGGNL 194
           GG+IGAR+G L+I  GGD  V + + PLFE LGK +T VG  G GQ+CK+ANQI +  N+
Sbjct: 122 GGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNI 181

Query: 195 MGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLG 254
             +SE L+FA +AG D  +  +A+ GG A S  +E+ GERMI+R F PG       KDL 
Sbjct: 182 EAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHGERMIKRTFNPGFKIALHQKDLN 241

Query: 255 MGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQ 303
           + +   +        + LP  A  ++LF+   ANG  +     L+  ++
Sbjct: 242 LALQSAK-----ALALNLPNTATCQELFNTCAANGGSQLDHSALVQALE 284

BLAST of CmaCh04G005210 vs. TrEMBL
Match: A0A0A0L752_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217150 PE=4 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 7.3e-147
Identity = 269/307 (87.62%), Postives = 285/307 (92.83%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           M+TPYP  I PTKTRIGWIGIGVMGAAMATRLL+AGYS++IYAR+PSKALDLQSKGA LV
Sbjct: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELAQVSDIVFTMVGHPSDVRQNVL++NG+LQGLNPGGVIVDTTSSHP LAR+IF A
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLF+ LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQ AI  NL+GLSEGLVFAERAGLD+K+FVEAVRGG A SMAMELF ERMI RDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGED ER+VVLPGAAL KQLFSAMVANGDGKFG QGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGED-ERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISV 300

Query: 301 VQRLNGK 308
           ++RLNGK
Sbjct: 301 IERLNGK 306

BLAST of CmaCh04G005210 vs. TrEMBL
Match: A0A059AT91_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02726 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.6e-130
Identity = 236/306 (77.12%), Postives = 268/306 (87.58%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METP P  I P +TRIGWIGIGVMGA+MA RL++AGY++ +YAR+PSKAL LQS GA L 
Sbjct: 1   METPCPTPINPARTRIGWIGIGVMGASMAARLISAGYALTVYARNPSKALPLQSLGALLA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           +S  ++A  SD+VFTMVGHPSDVR  VL  +G+L GLNPGG IVDTTSSHP LAREIF A
Sbjct: 61  DSPRDVAARSDVVFTMVGHPSDVRSIVLGTSGILSGLNPGGAIVDTTSSHPALAREIFDA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWA+DAPVSGGDIGARDGKLAIF GGD GVV+WLSPLF+ +GKVTY+G+AGCGQS
Sbjct: 121 ARGRDCWAIDAPVSGGDIGARDGKLAIFAGGDHGVVEWLSPLFDCMGKVTYMGKAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI +GGNL+GLSEG+VFAE+AGLDV+QFV+AVRGGAAGSM MELFGERMI +DFR
Sbjct: 181 CKIANQIVVGGNLLGLSEGMVFAEQAGLDVRQFVDAVRGGAAGSMVMELFGERMIGKDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVE GED E++VVLPGAA  KQLFS MVANGDG  GTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEGGED-EKVVVLPGAAQCKQLFSGMVANGDGHLGTQGLISV 300

Query: 301 VQRLNG 307
           +QR+NG
Sbjct: 301 IQRING 305

BLAST of CmaCh04G005210 vs. TrEMBL
Match: A0A0A0LC06_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214010 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 1.4e-129
Identity = 239/276 (86.59%), Postives = 255/276 (92.39%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I PTKTRIGWIGIGVMGAAMATRLL+AGYS++IYAR+PSKALDLQSKGA LV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELAQVSDIVFTMVGHPSDVRQNVL++NG+LQGLNPGGV+VDTTSSHP LAR+IF A
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLF+ LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQ AI  NL+GLSEGLVFAERAGLD+K+FVEAVRGG A SMAMELF ERMI RDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAAL 277
           PGGFAEYMVKDLG+GVDVVEEG+D ER+VVLPGA L
Sbjct: 241 PGGFAEYMVKDLGIGVDVVEEGDD-ERVVVLPGAPL 275

BLAST of CmaCh04G005210 vs. TrEMBL
Match: A0A067L2Z3_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05027 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 2.6e-128
Identity = 234/309 (75.73%), Postives = 268/309 (86.73%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           M+TPYP  I PT+TRIGWIGIG+MGAAMA+RLL+AGY+V +YAR+PSKA  LQ+KGA L 
Sbjct: 1   MDTPYPKPISPTETRIGWIGIGIMGAAMASRLLSAGYTVTVYARNPSKASPLQAKGAHLA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVL--EKNGLLQGLNPGGVIVDTTSSHPDLAREIF 120
           NS  ++A+ SDIVFTMVGHPSDVR  VL  +  G+++GLNPGGVIVD TSSHP LAREIF
Sbjct: 61  NSPYDVAKCSDIVFTMVGHPSDVRSIVLGSQGKGIIEGLNPGGVIVDATSSHPALAREIF 120

Query: 121 AAARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCG 180
            AARAKGCWAVD+PVSGGDIGARDGKLAI  GGD GVV WLSPLF  LGK TY+G AGCG
Sbjct: 121 DAARAKGCWAVDSPVSGGDIGARDGKLAILAGGDEGVVNWLSPLFGLLGKFTYMGPAGCG 180

Query: 181 QSCKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERD 240
           Q CKIANQI +G NL+GLSEGLVFAE+AGLD+K++++AVRGGAAGSM M+LFGERMI RD
Sbjct: 181 QHCKIANQIVVGANLLGLSEGLVFAEKAGLDMKKWIDAVRGGAAGSMVMDLFGERMIGRD 240

Query: 241 FRPGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLI 300
           FRPGGFAEYMVKD+GMGVDVVEE ED E++ VLPGAAL K LF+ MVANGDG  GTQGLI
Sbjct: 241 FRPGGFAEYMVKDMGMGVDVVEERED-EKVPVLPGAALGKHLFAGMVANGDGHLGTQGLI 300

Query: 301 SVVQRLNGK 308
           +V++RLNGK
Sbjct: 301 TVIERLNGK 308

BLAST of CmaCh04G005210 vs. TrEMBL
Match: M5XE29_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009081mg PE=4 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.8e-127
Identity = 237/308 (76.95%), Postives = 266/308 (86.36%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           M TPYP  I P++TRIGWIGIGVMGAAMA+RLL+AGYS+ IYAR+PSKA  LQS+GA L 
Sbjct: 1   MGTPYPNPISPSQTRIGWIGIGVMGAAMASRLLSAGYSLTIYARTPSKAHPLQSQGAQLA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLE-KNGLLQGLNPGGVIVDTTSSHPDLAREIFA 120
           +S  +LAQVSD+VFTMVGHP DVR N+L+ + GLL GL P  V VD TSSHP LAREIF 
Sbjct: 61  HSPFQLAQVSDVVFTMVGHPPDVRSNILDPETGLLWGLRPNSVSVDMTSSHPALAREIFN 120

Query: 121 AARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQ 180
           AARAK CW VDAPVSGGDIGAR+GKLAI  GGDA VV+WL+PLF+ LGKVTYVG AG GQ
Sbjct: 121 AARAKDCWTVDAPVSGGDIGAREGKLAILAGGDASVVEWLAPLFDVLGKVTYVGPAGSGQ 180

Query: 181 SCKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDF 240
           +CKIANQI +GGNL+GLSEGLVFAERAGLDV+QF+EAVRGGAAGSM MELFG+R+I RDF
Sbjct: 181 NCKIANQIVVGGNLLGLSEGLVFAERAGLDVQQFMEAVRGGAAGSMVMELFGDRIIGRDF 240

Query: 241 RPGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLIS 300
           RPGGFAEYMVKDLGMGVDVVEE E+  R+ VLPGAAL KQLFS MVANGDGK G QGLI+
Sbjct: 241 RPGGFAEYMVKDLGMGVDVVEENEE-GRVAVLPGAALCKQLFSGMVANGDGKLGGQGLIT 300

Query: 301 VVQRLNGK 308
           V++RLNGK
Sbjct: 301 VIERLNGK 307

BLAST of CmaCh04G005210 vs. TAIR10
Match: AT1G71180.1 (AT1G71180.1 6-phosphogluconate dehydrogenase family protein)

HSP 1 Score: 377.1 bits (967), Expect = 1.0e-104
Identity = 189/306 (61.76%), Postives = 236/306 (77.12%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I P+KTRIGWIGIG+MG+AM + ++AAGYSV +YAR   K  DLQ+KGA + 
Sbjct: 22  METPYPKLIDPSKTRIGWIGIGIMGSAMVSHIIAAGYSVTVYARDLRKTKDLQTKGARIA 81

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELA++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI A 
Sbjct: 82  NSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVTVDMTSSKPGLAREIHAE 141

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+G L IF GGD+ +V+WLSP+ + +G VTY+GEAG GQS
Sbjct: 142 ARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGTVTYMGEAGSGQS 201

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQIA   NL+GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE +++RD+R
Sbjct: 202 CKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEMIVKRDYR 261

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM  +             +PGAAL+KQLF+ MVANGDGK G QG++SV
Sbjct: 262 ATGFAEYMVKDLGMAAE-----------AAMPGAALSKQLFTGMVANGDGKLGIQGVVSV 316

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 322 IRRLNG 316

BLAST of CmaCh04G005210 vs. TAIR10
Match: AT1G71170.1 (AT1G71170.1 6-phosphogluconate dehydrogenase family protein)

HSP 1 Score: 364.8 bits (935), Expect = 5.4e-101
Identity = 182/306 (59.48%), Postives = 229/306 (74.84%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           MET YP  I P+KTRIGWIGIG+MG+AM + +LAAGYSV +YAR   K  DLQ+KG    
Sbjct: 1   METQYPKLIEPSKTRIGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  EL ++SD+VFT+VG+ +DVR  +L  +G+L GL PGGV VD TSS P LAREI+A 
Sbjct: 61  NSPKELGEMSDVVFTIVGNSNDVRSLLLGDDGVLSGLKPGGVTVDMTSSKPGLAREIYAE 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWAVDAPVSGGD GAR+GKL IF GGD+ +V+WL+P+ + +G V ++G AG GQS
Sbjct: 121 ARRRDCWAVDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVRFMGGAGSGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKI NQI +G N++GL+EG+VFAE+AGLD  +++EAV+ GAAGS  M LFGE M  RD++
Sbjct: 181 CKIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAGSAVMRLFGEMMAVRDYK 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
             GFAEYMVKDLGM           E  + +PG AL KQLF+ MVANGDGK G QG++ V
Sbjct: 241 ATGFAEYMVKDLGMAA---------EAAMAMPGTALNKQLFTVMVANGDGKLGFQGVVDV 297

Query: 301 VQRLNG 307
           ++RLNG
Sbjct: 301 IRRLNG 297

BLAST of CmaCh04G005210 vs. TAIR10
Match: AT4G29120.1 (AT4G29120.1 6-phosphogluconate dehydrogenase family protein)

HSP 1 Score: 294.7 bits (753), Expect = 6.9e-80
Identity = 151/303 (49.83%), Postives = 219/303 (72.28%), Query Frame = 1

Query: 9   IVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQ 68
           I P+ T+IGWIG GVMG +M   L+ AGY+V ++ R+ SKA  L   GA++ +S + +A+
Sbjct: 33  ITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTVFNRTISKAQTLIDMGANVADSPNSVAE 92

Query: 69  VSDIVFTMVGHPSDVRQNVLE-KNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCW 128
            SD+VFT+VG+PSDVR  +L+ K+G L GL  GGV+VD T+S P LA EI  AA  K C+
Sbjct: 93  QSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQGGVLVDMTTSEPSLAEEIAKAASFKNCF 152

Query: 129 AVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQSCKIANQI 188
           ++DAPVSGGD+GA++GKL+IF GGD   VK L PLF  +GKV ++G +G GQ  K+ANQI
Sbjct: 153 SIDAPVSGGDLGAKNGKLSIFAGGDETTVKRLDPLFSLMGKVNFMGTSGKGQFAKLANQI 212

Query: 189 AIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEY 248
            I   ++GL EGL++A +AGLDVK+F+EA+  GAAGS +++L+G+R+++RDF PG +  +
Sbjct: 213 TIASTMLGLVEGLIYAHKAGLDVKKFLEAISTGAAGSKSIDLYGDRILKRDFDPGFYVNH 272

Query: 249 MVKDLGMGVDVVEEGEDVERI-VVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQRLNG 308
            VKDLG+ ++      + +R+ + LPG ALA+QL+ ++ A+G+G  GTQ L+  ++RLN 
Sbjct: 273 FVKDLGICLN------ECQRMGLALPGLALAQQLYLSLKAHGEGDLGTQALLLALERLNN 329

Query: 309 KQV 310
             V
Sbjct: 333 VSV 329

BLAST of CmaCh04G005210 vs. TAIR10
Match: AT3G25530.1 (AT3G25530.1 glyoxylate reductase 1)

HSP 1 Score: 116.3 bits (290), Expect = 3.3e-26
Identity = 81/288 (28.12%), Postives = 141/288 (48.96%), Query Frame = 1

Query: 16  IGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFT 75
           +G++G+G+MG AM+  LL  G+ V ++ R+ SK  +L   GAS+  S +E+ +       
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 76  MVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAKGCWAVDAPVSG 135
           M+  P      V +K G+L+ +  G   +D ++   + + +I  A   KG   V+ PVSG
Sbjct: 63  MLSDPCAALSVVFDKGGVLEQICEGKGYIDMSTVDAETSLKINEAITGKGGRFVEGPVSG 122

Query: 136 GDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVT-YVGEAGCGQSCKIANQIAIGGNLM 195
               A DG+L I   GD  + +   P F+ LGK + Y+G+ G G   K+   + +G  + 
Sbjct: 123 SKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMKLIVNMIMGSMMN 182

Query: 196 GLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDLGM 255
             SEGLV A+++GL     ++ +  GA  +   +  G  M +  + P    ++  KD+ +
Sbjct: 183 AFSEGLVLADKSGLSSDTLLDILDLGAMTNPMFKGKGPSMNKSSYPPAFPLKHQQKDMRL 242

Query: 256 GVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISVVQ 303
            + + +     E  V +P AA A + F    + G G      +I  V+
Sbjct: 243 ALALGD-----ENAVSMPVAAAANEAFKKARSLGLGDLDFSAVIEAVK 285

BLAST of CmaCh04G005210 vs. TAIR10
Match: AT1G17650.1 (AT1G17650.1 glyoxylate reductase 2)

HSP 1 Score: 114.4 bits (285), Expect = 1.3e-25
Identity = 81/276 (29.35%), Postives = 132/276 (47.83%), Query Frame = 1

Query: 16  IGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLVNSASELAQVSDIVFT 75
           IG++G+G+MG+ MA  L+ AG  V ++ R+ SK   L   GA   +S  E+    D+ F 
Sbjct: 69  IGFLGMGIMGSPMAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFA 128

Query: 76  MVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAAARAK--GCWAVDAPV 135
           M+  P         KNG + G++ G   VD   S  D+A  I  + + K  G   ++APV
Sbjct: 129 MLADPESAIDVACGKNGAIFGISSGKGYVDV--STVDVASSILISKQIKDTGALFLEAPV 188

Query: 136 SGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVT-YVGEAGCGQSCKIANQIAIGGN 195
           SG    A DG+L     GD  + +  +P  + +GK   Y+GE G G + K+   + +G  
Sbjct: 189 SGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSM 248

Query: 196 LMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFRPGGFAEYMVKDL 255
           +   +EG++ +++ GLD    VE V  GA  +    L G  MI+  +      ++  KD+
Sbjct: 249 MASFAEGILLSQKVGLDPNVLVEVVSQGAINAPMYSLKGPSMIKSVYPTAFPLKHQQKDM 308

Query: 256 GMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANG 289
            + + + E           P AA A +L+    + G
Sbjct: 309 RLALGLAESVSQ-----STPIAAAANELYKVAKSYG 337

BLAST of CmaCh04G005210 vs. NCBI nr
Match: gi|659112135|ref|XP_008456080.1| (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis melo])

HSP 1 Score: 530.8 bits (1366), Expect = 1.6e-147
Identity = 271/307 (88.27%), Postives = 283/307 (92.18%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I PTKTRIGWIGIGVMGAAMATRLL+AGYSV IYARSPSKALDLQSKGA LV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSVTIYARSPSKALDLQSKGALLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELAQ SDIVFTMVGHPSDVRQNVL+ NG+LQGLNPGGVIVDTTSSHP LAR IF A
Sbjct: 61  NSVLELAQTSDIVFTMVGHPSDVRQNVLDDNGILQGLNPGGVIVDTTSSHPALARNIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLFE LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFEVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI I  NLMGLSEGL+FAERAGLD K+F+EAVRGGAAGSMAMELFGERMI RDFR
Sbjct: 181 CKIANQIPISANLMGLSEGLMFAERAGLDTKKFLEAVRGGAAGSMAMELFGERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGG+AEYMVKDLGM VD+VEEGED ER+VVLPGAAL KQLFSAMVANGDGKFG+QGLISV
Sbjct: 241 PGGYAEYMVKDLGMAVDIVEEGED-ERVVVLPGAALIKQLFSAMVANGDGKFGSQGLISV 300

Query: 301 VQRLNGK 308
           ++RLNGK
Sbjct: 301 IERLNGK 306

BLAST of CmaCh04G005210 vs. NCBI nr
Match: gi|778680236|ref|XP_004149969.2| (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus])

HSP 1 Score: 528.1 bits (1359), Expect = 1.0e-146
Identity = 269/307 (87.62%), Postives = 285/307 (92.83%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           M+TPYP  I PTKTRIGWIGIGVMGAAMATRLL+AGYS++IYAR+PSKALDLQSKGA LV
Sbjct: 1   MQTPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELAQVSDIVFTMVGHPSDVRQNVL++NG+LQGLNPGGVIVDTTSSHP LAR+IF A
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVIVDTTSSHPALARDIFIA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLF+ LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQ AI  NL+GLSEGLVFAERAGLD+K+FVEAVRGG A SMAMELF ERMI RDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVEEGED ER+VVLPGAAL KQLFSAMVANGDGKFG QGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEEGED-ERVVVLPGAALTKQLFSAMVANGDGKFGNQGLISV 300

Query: 301 VQRLNGK 308
           ++RLNGK
Sbjct: 301 IERLNGK 306

BLAST of CmaCh04G005210 vs. NCBI nr
Match: gi|470127985|ref|XP_004299936.1| (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Fragaria vesca subsp. vesca])

HSP 1 Score: 474.2 bits (1219), Expect = 1.8e-130
Identity = 240/307 (78.18%), Postives = 265/307 (86.32%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           M TPYP  I P KTRIGWIGIGVMGAAMA+RLL+AGYS+ +YAR+PSKA  LQS+GA L 
Sbjct: 1   MGTPYPNPISPAKTRIGWIGIGVMGAAMASRLLSAGYSLTVYARTPSKAAPLQSQGAHLA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           +S  +LAQ+SD+VFTMVGHPSDVR N L  N LL  LNP  V VD TSSHP LAREIFAA
Sbjct: 61  DSPFQLAQLSDVVFTMVGHPSDVRSNYLSPNALLSSLNPNSVTVDMTSSHPALAREIFAA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           +RAK CW+VDAPVSGGDIGAR+GKLAI  GGDA VV WL+PLFE +GKVTY+G AG GQS
Sbjct: 121 SRAKHCWSVDAPVSGGDIGAREGKLAILAGGDASVVTWLAPLFEIMGKVTYMGPAGSGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI +G NL+GLSEGLVFAERAGLDVK+F+EAVRGGAAGS  MELFGERMIERDFR
Sbjct: 181 CKIANQITVGANLLGLSEGLVFAERAGLDVKRFMEAVRGGAAGSKVMELFGERMIERDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKD+GMGVDVVEEGED  R+VVLPGAAL KQLFS MVANGDGK GTQGLI+ 
Sbjct: 241 PGGFAEYMVKDMGMGVDVVEEGED-GRVVVLPGAALCKQLFSGMVANGDGKLGTQGLITA 300

Query: 301 VQRLNGK 308
           ++RLNGK
Sbjct: 301 IERLNGK 306

BLAST of CmaCh04G005210 vs. NCBI nr
Match: gi|702468926|ref|XP_010030108.1| (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Eucalyptus grandis])

HSP 1 Score: 473.8 bits (1218), Expect = 2.3e-130
Identity = 236/306 (77.12%), Postives = 268/306 (87.58%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METP P  I P +TRIGWIGIGVMGA+MA RL++AGY++ +YAR+PSKAL LQS GA L 
Sbjct: 1   METPCPTPINPARTRIGWIGIGVMGASMAARLISAGYALTVYARNPSKALPLQSLGALLA 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           +S  ++A  SD+VFTMVGHPSDVR  VL  +G+L GLNPGG IVDTTSSHP LAREIF A
Sbjct: 61  DSPRDVAARSDVVFTMVGHPSDVRSIVLGTSGILSGLNPGGAIVDTTSSHPALAREIFDA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           AR + CWA+DAPVSGGDIGARDGKLAIF GGD GVV+WLSPLF+ +GKVTY+G+AGCGQS
Sbjct: 121 ARGRDCWAIDAPVSGGDIGARDGKLAIFAGGDHGVVEWLSPLFDCMGKVTYMGKAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQI +GGNL+GLSEG+VFAE+AGLDV+QFV+AVRGGAAGSM MELFGERMI +DFR
Sbjct: 181 CKIANQIVVGGNLLGLSEGMVFAEQAGLDVRQFVDAVRGGAAGSMVMELFGERMIGKDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAALAKQLFSAMVANGDGKFGTQGLISV 300
           PGGFAEYMVKDLGMGVDVVE GED E++VVLPGAA  KQLFS MVANGDG  GTQGLISV
Sbjct: 241 PGGFAEYMVKDLGMGVDVVEGGED-EKVVVLPGAAQCKQLFSGMVANGDGHLGTQGLISV 300

Query: 301 VQRLNG 307
           +QR+NG
Sbjct: 301 IQRING 305

BLAST of CmaCh04G005210 vs. NCBI nr
Match: gi|778680148|ref|XP_011651257.1| (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cucumis sativus])

HSP 1 Score: 470.7 bits (1210), Expect = 2.0e-129
Identity = 239/276 (86.59%), Postives = 255/276 (92.39%), Query Frame = 1

Query: 1   METPYPISIVPTKTRIGWIGIGVMGAAMATRLLAAGYSVAIYARSPSKALDLQSKGASLV 60
           METPYP  I PTKTRIGWIGIGVMGAAMATRLL+AGYS++IYAR+PSKALDLQSKGA LV
Sbjct: 1   METPYPNPISPTKTRIGWIGIGVMGAAMATRLLSAGYSLSIYARTPSKALDLQSKGALLV 60

Query: 61  NSASELAQVSDIVFTMVGHPSDVRQNVLEKNGLLQGLNPGGVIVDTTSSHPDLAREIFAA 120
           NS  ELAQVSDIVFTMVGHPSDVRQNVL++NG+LQGLNPGGV+VDTTSSHP LAR+IF A
Sbjct: 61  NSVLELAQVSDIVFTMVGHPSDVRQNVLDENGILQGLNPGGVVVDTTSSHPALARDIFTA 120

Query: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDAGVVKWLSPLFEALGKVTYVGEAGCGQS 180
           ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGD GVV WL PLF+ LGKVTYVGEAGCGQS
Sbjct: 121 ARAKGCWAVDAPVSGGDIGARDGKLAIFGGGDEGVVNWLLPLFQVLGKVTYVGEAGCGQS 180

Query: 181 CKIANQIAIGGNLMGLSEGLVFAERAGLDVKQFVEAVRGGAAGSMAMELFGERMIERDFR 240
           CKIANQ AI  NL+GLSEGLVFAERAGLD+K+FVEAVRGG A SMAMELF ERMI RDFR
Sbjct: 181 CKIANQFAISANLLGLSEGLVFAERAGLDMKKFVEAVRGGGAWSMAMELFAERMIGRDFR 240

Query: 241 PGGFAEYMVKDLGMGVDVVEEGEDVERIVVLPGAAL 277
           PGGFAEYMVKDLG+GVDVVEEG+D ER+VVLPGA L
Sbjct: 241 PGGFAEYMVKDLGIGVDVVEEGDD-ERVVVLPGAPL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
3HID3_ARATH1.9e-10361.76Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial OS=Arabidopsis... [more]
3HID2_ARATH9.6e-10059.48Probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial OS=Arabidopsis... [more]
3HID1_ARATH1.2e-7849.83Probable 3-hydroxyisobutyrate dehydrogenase-like 1, mitochondrial OS=Arabidopsis... [more]
YKWC_BACSU1.2e-5437.98Uncharacterized oxidoreductase YkwC OS=Bacillus subtilis (strain 168) GN=ykwC PE... [more]
GLXR_ECOLI3.1e-4237.372-hydroxy-3-oxopropionate reductase OS=Escherichia coli (strain K12) GN=glxR PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0L752_CUCSA7.3e-14787.62Uncharacterized protein OS=Cucumis sativus GN=Csa_3G217150 PE=4 SV=1[more]
A0A059AT91_EUCGR1.6e-13077.12Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I02726 PE=4 SV=1[more]
A0A0A0LC06_CUCSA1.4e-12986.59Uncharacterized protein OS=Cucumis sativus GN=Csa_3G214010 PE=4 SV=1[more]
A0A067L2Z3_JATCU2.6e-12875.73Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05027 PE=4 SV=1[more]
M5XE29_PRUPE3.8e-12776.95Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009081mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G71180.11.0e-10461.76 6-phosphogluconate dehydrogenase family protein[more]
AT1G71170.15.4e-10159.48 6-phosphogluconate dehydrogenase family protein[more]
AT4G29120.16.9e-8049.83 6-phosphogluconate dehydrogenase family protein[more]
AT3G25530.13.3e-2628.13 glyoxylate reductase 1[more]
AT1G17650.11.3e-2529.35 glyoxylate reductase 2[more]
Match NameE-valueIdentityDescription
gi|659112135|ref|XP_008456080.1|1.6e-14788.27PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cu... [more]
gi|778680236|ref|XP_004149969.2|1.0e-14687.62PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cu... [more]
gi|470127985|ref|XP_004299936.1|1.8e-13078.18PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Fr... [more]
gi|702468926|ref|XP_010030108.1|2.3e-13077.12PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 2, mitochondrial [Eu... [more]
gi|778680148|ref|XP_011651257.1|2.0e-12986.59PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial [Cu... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR0061156PGDH_NADP-bd
IPR0089276-PGluconate_DH-like_C_sf
IPR0133286PGD_dom2
IPR015815HIBADH-related
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0051287NAD binding
GO:0004616phosphogluconate dehydrogenase (decarboxylating) activity
GO:0016491oxidoreductase activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019521 D-gluconate metabolic process
biological_process GO:0046487 glyoxylate metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006098 pentose-phosphate shunt
cellular_component GO:0005575 cellular_component
molecular_function GO:0008679 2-hydroxy-3-oxopropionate reductase activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0004616 phosphogluconate dehydrogenase (decarboxylating) activity
molecular_function GO:0016491 oxidoreductase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh04G005210.1CmaCh04G005210.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0061156-phosphogluconate dehydrogenase, NADP-bindingPFAMPF03446NAD_binding_2coord: 15..173
score: 1.4
IPR0089276-phosphogluconate dehydrogenase C-terminal domain-likeunknownSSF481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 174..308
score: 6.57
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3DG3DSA:1.10.1040.10coord: 176..307
score: 1.7
IPR0158153-hydroxyisobutyrate dehydrogenase-relatedPIRPIRSF000103HIBADHcoord: 10..310
score: 1.1
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 13..174
score: 2.0
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 14..172
score: 1.92
NoneNo IPR availablePANTHERPTHR229813-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATEDcoord: 1..306
score: 3.0E
NoneNo IPR availablePANTHERPTHR22981:SF543-HYDROXYISOBUTYRATE DEHYDROGENASE-LIKE 2, MITOCHONDRIAL-RELATEDcoord: 1..306
score: 3.0E

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None