CmaCh03G008760 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh03G008760
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Cysteine desulfurase IscS
Location	Cma_Chr03 : 6469792 .. 6472934 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGGTGCTCGATGCTAGGCTTCCTTATTATCTCTCTCGATATGGTAATCCACCACTCTCAGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGGTAACGATTATTTAAGTTCTTAATCTTTGTCTCTTTACTTTTTCTTCCACCTGATTCATCAAAGGACAATTTAGCTAAATGCCTTTTATTTTCTTCTCCCTTCTTTCTGGTTGAATAGGTATGAATTTCTGGTTTCTCTTCCTTTTTTACCGATATGTAGCAGCTTACATTTCATTCACATGTTTTTTTTTGTAAGGCGCACAACTTTTTATTAAAGACGATGAGACATCCTCTCGTCTCACTCCCAGCTGACTAAGATTACAGAAATTTCTTAATCAAGAAGACAAGAATGGTTATGAAATCATCCTTTAGACAAAGGAATAGCCTTCAATTGGCTTAGATTTCATCGATATGATGTTCAGATTAATTTTAGACTCATGGTTCCACTTCTACCAATGCAAGATGAATGGGTGTGATGGTACACGCCTTAAGTTGATGAATAGTTTAGTCTCATGGAGCAACGTTTATTGGGGTAAAAGGCTAGTGAAAATCCTTATAGTTTTTAAGACCATGACACCTAGACAGTTATACAATTCTCATTCTTAACCTGATACGTCTGGACCGTGAAGTTTTTGTATGAACAGCAACTTTATGCAAGTAAAATGGCATGTGCAGTTTTGTCGCCATCTGCAGTGACACTCTGGATCAAGAAGACCCTGGCTATACCATTATTAATATTGCCTCTTCTAGATTAGGACTCTCTTTATTTATGTAAGCACTTGCTTCCATAAGGTTATTCTTTCATTCAATTTCAACTTCTTGCAGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTAGACTGTATTAAGCCTTTACTATAGCTTTTTAGGATAAATCAGTTGAAAATTCTGTGCACGGTCATTGACAGCTATGATGATCTTACACGTTTGGATTTTATGCATGCTTCTCTGATTATAATGTTATAAAATTAACATTCTAAATCTTCCTATAAAAAACTAACATCCTAAATCCACGTCTTCTGCTGTTTAAAATGCAGTGAAACTTTCTAGGTACAAAACCAATAAGGAGATTCTAAAGAGAACTCTTTCAGTAGATCATTTTAAAAAAAAAAAAAAATTATTTATTTATTCATTTGTTGCAGTATTGTTGGTTGTCATGAATCCACACGTGTTAGGATATACATCTATTTCTACGAATATGTGTTCTAGAATGAACATCCCAAGTCCATGTCGTCTGATTTCCAAAATGAAGTGGAACTTTGTTGGACTTATTCCTTCTGGGTTTGCTATTGCCTGAAAGTTTGGTCATGAAAGGTGCAACCTTTTCTTGTTGGTCTGACTTTCTCATCAAGGAAAAGGTTCTTTGCCCTGTGGAACTTGAAACAGTGAAGCGTTCTATGTTCAAGGGGCTAGCCACTCTGCAGGAAGCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCACACAAAAAAAAAAAAGGAAGTGATCCTGGCTTCTAGGCGTGCTGTAGCGAATATGAACATGGGTGTGGTAAATTGAAATTTCAGATTTCCATGCGGATAAGAGCAAGAAGTGATTGAATGGCCATGTCTATGAAGTTCAAACTACTTGCCCAGTTGAGACTGTGTAGGAGGCCCAGGAGGCGCAGTTTAGTATCCTCTATTCTGTTTTGGACTTTCACTGTTTGGTTGTACACAAATGAGGACAATAAAATTAGTGAATGTTTTAGTGTTCCGCTAACAGTTTGCTAACACCAATACAGTTGTCTTGGTACTGCGTTCTTTTTTATGTTTATAAGTGAAGATGTCAACGAAACCTCTTTATTCATGAGCAGTGTACAACTTATTCTGTACAGCATCAAATTTATACCGACTTGGAACTTTATGCATCCTTGATACTATTTTATTTTCTTCTACAAAATGGAAGTTTTCATTTAGATATGAAAACTAGTACCTCTGATAGCAATATCATCACGAGCCAGCAGGACCCTGGAGACCTTGAAACAAGCTGTGAAAATCTTCTTGCTCCTGACAGAATCAATACATAATAGAAAGTTAGAAGAAACCAGTGGGTGGGGGTTTGAAATGGTGGGTGGGTGTGTGTGCTTGGTCTTTAATTGAGAGTGCATTATTTTTTGAAACATGCAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCTGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCTGTGCAGTGCAGTGGCCAAGTTTTGGGCAACCTGACTGAAGGCTTTCTTTTTTGTAAAATATGTGGAAGAAGAAGCCAAGGAAGATAGCAATATACGGTATCTGATTGGGAAAGTGACTCATTTCATTTATGAGATGAGAATCATGGCAATATCAGTTGTGGCCGTGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGGGG

mRNA sequence

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTACTGTGTAGCAATATCCATTAAATGTCCTTGTCTTCTGAGAAGAAAGCAACTGCTCAAAAGGAGAGGGGGTTCAGCAGAAGAAGCGCTGTGCAGTGCAGTGGCCAAGTTTTGGGCAACCTGACTGAAGGCTTTCTTTTTTGTAAAATATGTGGAAGAAGAAGCCAAGGAAGATAGCAATATACGGTATCTGATTGGGAAAGTGACTCATTTCATTTATGAGATGAGAATCATGGCAATATCAGTTGTGGCCGTGGGGAGAAGGATTATGGGTATGGCCCAGCTGGGAAAGTGGGG

Coding sequence (CDS)

ATGGCGTCCAAGATTCTTGCATCCAGCCTACGCCTCGCCCGCACAAATGTCTCTCCCGCCGTCTCCCGCTTCCGGCCTCTCTCCACCGCGGCTGCTGTAGCCACTTCTCCGTCGGAACCCCATAAGGATGAAACTGGGATTTCTATGAAAGGTGTTAAAATCTCTGGACGACCGATTTATCTTGATATGCAAGCTACATCCCCTGCTGATACTCGGACGCATCTCTATGGTTGGGAATCGGACCATGCCGTCGAGACGGCTTGCTCCCATGTTGCTGCTCTTATTGGTGCCTCGCCTAAGGAGATTGTGTTGACCTCTGGAGCTACCGAGTCCAATAACATATCCATCAAGGGGGTCATGAATTTCTACAGAGAGAAGAAAAGGCACTTGATTACCATTCAGACGGAGCACAAGTGCGTTCTTGATTCCTGCAGACATTTGCAGCAGGTGGGTTTTGAGATTACCTACTTACCGGTTGGGTCCGATGGAATTGTTGATTTGGATAAGCTCCGTTCTGCGATTAGACCCGACACTGGCCTTGTTTGTACTTTGTGCTTGATATGCAGATATAGAACAACCCCCTCCTCCTCCTCCTCCAGCAGGATTGTGATTAGAATAAACTCTGAATCTTACAAATGCATTCTTAAGAAGGTTGCAGAGCTTCACTCCCATGGCTTTCTTAGGTGCACGGGACGTATGCTAGCCGTGCTGCAGGTACTGTGTAGCAATATCCATTAA

Protein sequence

MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIYLDMQATSPADTRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKLRSAIRPDTGLVCTLCLICRYRTTPSSSSSSRIVIRINSESYKCILKKVAELHSHGFLRCTGRMLAVLQVLCSNIH

Homology

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BLAST of CmaCh03G008760 vs. Swiss-Prot
Match: MNIF1_ARATH (Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana GN=NIFS1 PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 8.7e-56
Identity = 115/205 (56.10%), Postives = 148/205 (72.20%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK++++++R   T      SR R LSTAAA     +E + ++  I MKGV+ISGRP+Y
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQAT+P D                   +RTHLYGWE+++AVE A + VA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV  SGATE+NN+++KGVM+FY++ K+H+IT QTEHKCVLDSCRHLQQ GFE+TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG+VDL+ LR AIRPDTGLV  + +
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAV 201

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BLAST of CmaCh03G008760 vs. Swiss-Prot
Match: NFS1_ARTBC (Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) GN=ARB_05732-2 PE=3 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 5.5e-42
Identity = 95/191 (49.74%), Postives = 118/191 (61.78%), Query Frame = 1


Query: 13  ARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISG-RPIYLDMQATSPAD- 72
           A+T V  AV   +  S      T  +  +     +    +   G RPIYLDMQAT+P D 
Sbjct: 57  AQTTVESAVKTEQKASVPGTAVTGDALANPSAGILKQATIMDEGNRPIYLDMQATTPTDP 116

Query: 73  ------------------TRTHLYGWESDHAVETACSHVAALIGASPKEIVLTSGATESN 132
                             +RTH YGWE++ A E A SHVA LIGA PKEI+ TSGATESN
Sbjct: 117 RVLDAMLPFLTGLYGNPHSRTHAYGWETEKATEQARSHVATLIGADPKEIIFTSGATESN 176

Query: 133 NISIKGVMNFYRE--KKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSDGIVDLDKL 182
           N+SIKGV  F+    KK+H+IT QTEHKCVLDSCRHLQ  GFE+TYLPV S+G++ ++ L
Sbjct: 177 NMSIKGVARFFGRSGKKKHIITTQTEHKCVLDSCRHLQDEGFEVTYLPVQSNGLIKIEDL 236

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BLAST of CmaCh03G008760 vs. Swiss-Prot
Match: NFS1_DROME (Probable cysteine desulfurase, mitochondrial OS=Drosophila melanogaster GN=CG12264 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-41
Identity = 96/199 (48.24%), Postives = 121/199 (60.80%), Query Frame = 1


Query: 4   KILASSLRLARTNVSPAVSRFRPLS--TAAAVATSPSEPHKDETGISMKGVKISGRPIYL 63
           +I A  LR   TN    V R    S  TAA  A +  E  + +   ++K  +  GRP+YL
Sbjct: 7   RIQAQVLRSRATNALANVVRHEATSSRTAAKPAATSKEFRERQVRFNIKNEQTEGRPLYL 66

Query: 64  DMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKEI 123
           D QAT+P D                   +RTH YGWE++ AVE A   VA LIGA PKEI
Sbjct: 67  DAQATTPMDPRVLDAMLPYLTNFYGNPHSRTHAYGWETESAVEKAREQVATLIGADPKEI 126

Query: 124 VLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGSD 182
           + TSGATESNNI++KGV  FY  KKRH+IT QTEHKCVLDSCR L+  GF++TYLPV ++
Sbjct: 127 IFTSGATESNNIAVKGVARFYGTKKRHVITTQTEHKCVLDSCRALENEGFKVTYLPVLAN 186

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BLAST of CmaCh03G008760 vs. Swiss-Prot
Match: NFS1_HUMAN (Cysteine desulfurase, mitochondrial OS=Homo sapiens GN=NFS1 PE=1 SV=3)

HSP 1 Score: 170.2 bits (430), Expect = 2.7e-41
Identity = 83/147 (56.46%), Postives = 104/147 (70.75%), Query Frame = 1


Query: 57  RPIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGA 116
           RP+Y+D+QAT+P D                   +RTH YGWES+ A+E A   VA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYL 176
            P+EI+ TSGATESNNI+IKGV  FYR +K+HLIT QTEHKCVLDSCR L+  GF++TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRPDTGLVCTL 185
           PV   GI+DL +L +AI+PDT LV  +
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVM 203

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BLAST of CmaCh03G008760 vs. Swiss-Prot
Match: NFS1_PONAB (Cysteine desulfurase, mitochondrial OS=Pongo abelii GN=NFS1 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.7e-41
Identity = 83/147 (56.46%), Postives = 104/147 (70.75%), Query Frame = 1


Query: 57  RPIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGA 116
           RP+Y+D+QAT+P D                   +RTH YGWES+ A+E A   VA+LIGA
Sbjct: 57  RPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGA 116

Query: 117 SPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYL 176
            P+EI+ TSGATESNNI+IKGV  FYR +K+HLIT QTEHKCVLDSCR L+  GF++TYL
Sbjct: 117 DPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYL 176

Query: 177 PVGSDGIVDLDKLRSAIRPDTGLVCTL 185
           PV   GI+DL +L +AI+PDT LV  +
Sbjct: 177 PVQKSGIIDLKELEAAIQPDTSLVSVM 203

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BLAST of CmaCh03G008760 vs. TrEMBL
Match: A0A0A0KIM0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497010 PE=3 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 4.8e-77
Identity = 158/206 (76.70%), Postives = 170/206 (82.52%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAA-VATSPSEPHKDETGISMKGVKISGRPI 60
           MASKILAS+LR   +N S  +SRFRP+STAAA VA SPSEPH+DETGISMKGVKISGRP+
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPK 120
           YLDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVG 180
           EIV TSGATESNNISIKGVM+FYR+KKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLDKLRSAIRPDTGLVCTLCL 187
           SDGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAV 206

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BLAST of CmaCh03G008760 vs. TrEMBL
Match: A0A059ALR2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I00651 PE=3 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.5e-62
Identity = 132/206 (64.08%), Postives = 159/206 (77.18%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKD-ETGISMKGVKISGRPI 60
           MASK+LAS+LR +  + +P     R LSTAAAVA    EP+++  +G+ MKGV+ISGRP+
Sbjct: 2   MASKLLASALRRSSPSTAPPPPLRRLLSTAAAVA----EPYEEASSGVVMKGVRISGRPL 61

Query: 61  YLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPK 120
           YLDMQATSP D                   +RTHLYGWESD AVE+A + VAAL+GASPK
Sbjct: 62  YLDMQATSPVDPRVLDAMLPFYLHRYGNPHSRTHLYGWESDLAVESARARVAALVGASPK 121

Query: 121 EIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVG 180
           E+V TSGATESNNIS+KGVM+FYR+KKRH+IT QTEHKCVLDSCRHLQQ GF++TYLPVG
Sbjct: 122 ELVFTSGATESNNISVKGVMHFYRDKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPVG 181

Query: 181 SDGIVDLDKLRSAIRPDTGLVCTLCL 187
            DGI+DLD+LRSAIRPDTGLV  + +
Sbjct: 182 PDGIIDLDRLRSAIRPDTGLVSVMAV 203

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BLAST of CmaCh03G008760 vs. TrEMBL
Match: A0A140B487_PRUPE (Cysteine desulfurase 14 OS=Prunus persica var. persica GN=NFS1 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.3e-62
Identity = 133/207 (64.25%), Postives = 157/207 (75.85%), Query Frame = 1


Query: 1   MASKILASSLRLARTN--VSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRP 60
           MASK+LAS+LR   T    S A    R LSTAAAVA +  EP++D  GISMKGVKISGRP
Sbjct: 1   MASKLLASALRQTLTPKPTSTASHLARALSTAAAVAVA--EPYEDADGISMKGVKISGRP 60

Query: 61  IYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASP 120
           +YLDMQATSP D                   +RTH+YGWESD AVETA + +A LIG+SP
Sbjct: 61  LYLDMQATSPVDPRVVDAMLPYYLTRYGNPHSRTHMYGWESDSAVETARAQIADLIGSSP 120

Query: 121 KEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPV 180
           KEIV TSGATE NNIS+KGVM+FY++KKRH+IT QTEHKCVLDSCRHLQQ GF++TYLPV
Sbjct: 121 KEIVFTSGATECNNISVKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 180

Query: 181 GSDGIVDLDKLRSAIRPDTGLVCTLCL 187
            SDG++DLD+LR+AIRPDTGLV  + +
Sbjct: 181 KSDGLIDLDELRAAIRPDTGLVSVMAV 205

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BLAST of CmaCh03G008760 vs. TrEMBL
Match: M5WHW3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005512mg PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 4.3e-62
Identity = 133/207 (64.25%), Postives = 157/207 (75.85%), Query Frame = 1


Query: 1   MASKILASSLRLARTN--VSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRP 60
           MASK+LAS+LR   T    S A    R LSTAAAVA +  EP++D  GISMKGVKISGRP
Sbjct: 1   MASKLLASALRQTLTPKPTSTASHLARALSTAAAVAVA--EPYEDADGISMKGVKISGRP 60

Query: 61  IYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASP 120
           +YLDMQATSP D                   +RTH+YGWESD AVETA + +A LIG+SP
Sbjct: 61  LYLDMQATSPVDPRVVDAMLPYYLTRYGNPHSRTHMYGWESDSAVETARAQIADLIGSSP 120

Query: 121 KEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPV 180
           KEIV TSGATE NNIS+KGVM+FY++KKRH+IT QTEHKCVLDSCRHLQQ GF++TYLPV
Sbjct: 121 KEIVFTSGATECNNISVKGVMHFYKDKKRHVITTQTEHKCVLDSCRHLQQEGFDVTYLPV 180

Query: 181 GSDGIVDLDKLRSAIRPDTGLVCTLCL 187
            SDG++DLD+LR+AIRPDTGLV  + +
Sbjct: 181 KSDGLIDLDELRAAIRPDTGLVSVMAV 205

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BLAST of CmaCh03G008760 vs. TrEMBL
Match: W9RYP8_9ROSA (Cysteine desulfurase 1 OS=Morus notabilis GN=L484_007761 PE=3 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 9.7e-62
Identity = 136/208 (65.38%), Postives = 156/208 (75.00%), Query Frame = 1


Query: 1   MASKILASSLR--LARTNVSPAV-SRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGR 60
           MASK+ AS+LR  L RT  S AV S  R  STAAAVA  P E   D  GI+MKGV+ISGR
Sbjct: 1   MASKLFASNLRHALKRTTASGAVFSNLRATSTAAAVAPEPLEETTD--GINMKGVRISGR 60

Query: 61  PIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGAS 120
           P+YLD+QATSP D                   +RTHLYGWE+D AVE A + VA LIGAS
Sbjct: 61  PLYLDVQATSPVDPRVLDAMMPFYLSRYGNPHSRTHLYGWEADEAVENARAQVAELIGAS 120

Query: 121 PKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLP 180
           PKEIV TSGATE NNI++KGVM+FY+EKKRH+IT QTEHKCVLDSCRHLQQ GFEITYLP
Sbjct: 121 PKEIVFTSGATECNNIAVKGVMHFYKEKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLP 180

Query: 181 VGSDGIVDLDKLRSAIRPDTGLVCTLCL 187
           VGSDG+VDL+KL+S+IRPDTGLV  + +
Sbjct: 181 VGSDGLVDLEKLKSSIRPDTGLVSVMAV 206

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BLAST of CmaCh03G008760 vs. TAIR10
Match: AT5G65720.1 (AT5G65720.1 nitrogen fixation S (NIFS)-like 1)

HSP 1 Score: 218.4 bits (555), Expect = 4.9e-57
Identity = 115/205 (56.10%), Postives = 148/205 (72.20%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK++++++R   T      SR R LSTAAA     +E + ++  I MKGV+ISGRP+Y
Sbjct: 1   MASKVISATIRRTLTKPHGTFSRCRYLSTAAAA----TEVNYEDESIMMKGVRISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQAT+P D                   +RTHLYGWE+++AVE A + VA LI ASPKE
Sbjct: 61  LDMQATTPIDPRVFDAMNASQIHEYGNPHSRTHLYGWEAENAVENARNQVAKLIEASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV  SGATE+NN+++KGVM+FY++ K+H+IT QTEHKCVLDSCRHLQQ GFE+TYLPV +
Sbjct: 121 IVFVSGATEANNMAVKGVMHFYKDTKKHVITTQTEHKCVLDSCRHLQQEGFEVTYLPVKT 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG+VDL+ LR AIRPDTGLV  + +
Sbjct: 181 DGLVDLEMLREAIRPDTGLVSIMAV 201

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: gi|659079814|ref|XP_008440458.1| (PREDICTED: cysteine desulfurase 1, mitochondrial [Cucumis melo])

HSP 1 Score: 305.4 bits (781), Expect = 8.7e-80
Identity = 161/205 (78.54%), Postives = 170/205 (82.93%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASKILAS LR   TN SP +SRFRP+STAAAVA SPSEPH+DETGISMKGVKISGRP+Y
Sbjct: 1   MASKILASCLRRTLTNSSPPISRFRPISTAAAVAASPSEPHEDETGISMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPKE
Sbjct: 61  LDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATESNNISIKGVM+FYREKKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVGS
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVGS 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 DGIVDLEKLRSAIRPDTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: gi|778718827|ref|XP_011657914.1| (PREDICTED: cysteine desulfurase, mitochondrial [Cucumis sativus])

HSP 1 Score: 295.8 bits (756), Expect = 6.9e-77
Identity = 158/206 (76.70%), Postives = 170/206 (82.52%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAA-VATSPSEPHKDETGISMKGVKISGRPI 60
           MASKILAS+LR   +N S  +SRFRP+STAAA VA SPSEPH+DETGISMKGVKISGRP+
Sbjct: 1   MASKILASTLRRTLSNSSSPISRFRPISTAAAAVAASPSEPHEDETGISMKGVKISGRPL 60

Query: 61  YLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPK 120
           YLDMQATSP D                   +RTHLYGWESDHAVETA S VAALIGASPK
Sbjct: 61  YLDMQATSPVDPRVLDAMLPYYISRYGNPHSRTHLYGWESDHAVETARSQVAALIGASPK 120

Query: 121 EIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVG 180
           EIV TSGATESNNISIKGVM+FYR+KKRHLIT QTEHKCVLDSCRHLQQ GFEITYLPVG
Sbjct: 121 EIVFTSGATESNNISIKGVMHFYRDKKRHLITTQTEHKCVLDSCRHLQQEGFEITYLPVG 180

Query: 181 SDGIVDLDKLRSAIRPDTGLVCTLCL 187
           SDGIVDL+KLRSAIRPDTGLV  + +
Sbjct: 181 SDGIVDLEKLRSAIRPDTGLVSVMAV 206

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: gi|1021516189|ref|XP_016201576.1| (PREDICTED: cysteine desulfurase, mitochondrial [Arachis ipaensis])

HSP 1 Score: 252.3 bits (643), Expect = 8.7e-64
Identity = 132/205 (64.39%), Postives = 156/205 (76.10%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           M SK+LAS+LR   T  +  + R +PLSTA A A   S+P++D  GI+MKGVKISGRP+Y
Sbjct: 1   MTSKLLASNLRHHLTRSARILFRSQPLSTATAAAADSSQPYEDAAGITMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LD+QATSP D                   +RTH YGWESD AVE A S VAAL+GASPKE
Sbjct: 61  LDVQATSPVDPRVLDAMLPFFLSRYGNPHSRTHFYGWESDEAVERARSQVAALVGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATESNNISIKGVM+FYREKK+H+IT QTEHKCVLDSCR+LQQ GF++TYLPV S
Sbjct: 121 IVFTSGATESNNISIKGVMHFYREKKKHVITTQTEHKCVLDSCRYLQQEGFDVTYLPVES 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG+VDL+KLR+AIRPDTGLV  + +
Sbjct: 181 DGLVDLEKLRAAIRPDTGLVSVMAV 205

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: gi|764517110|ref|XP_011466445.1| (PREDICTED: cysteine desulfurase 1, mitochondrial [Fragaria vesca subsp. vesca])

HSP 1 Score: 252.3 bits (643), Expect = 8.7e-64
Identity = 137/205 (66.83%), Postives = 156/205 (76.10%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAAVATSPSEPHKDETGISMKGVKISGRPIY 60
           MASK+LASSLR   T   P+ S FRPLSTAAA A   +E + D  G+SMKGVKISGRP+Y
Sbjct: 1   MASKLLASSLRKTLTP-KPSSSFFRPLSTAAAAA---AEVYDDPAGVSMKGVKISGRPLY 60

Query: 61  LDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGASPKE 120
           LDMQATSP D                   +RTH+YGWESD AVETA + VA LIGASPKE
Sbjct: 61  LDMQATSPVDPRVVDAMLPYYLTRYGNPHSRTHMYGWESDSAVETARAQVADLIGASPKE 120

Query: 121 IVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYLPVGS 180
           IV TSGATE NNIS+KGVM+FYR+KKRH+IT QTEHKCVLDSCRHLQQ GFEITYLPV  
Sbjct: 121 IVFTSGATECNNISVKGVMHFYRDKKRHVITTQTEHKCVLDSCRHLQQEGFEITYLPVKP 180

Query: 181 DGIVDLDKLRSAIRPDTGLVCTLCL 187
           DG++DLD+LR+AIRPDTGLV  + +
Sbjct: 181 DGLIDLDQLRAAIRPDTGLVSVMAV 201

[Close]

BLAST of CmaCh03G008760 vs. NCBI nr
Match: gi|1009145637|ref|XP_015890440.1| (PREDICTED: cysteine desulfurase, mitochondrial [Ziziphus jujuba])

HSP 1 Score: 251.9 bits (642), Expect = 1.1e-63
Identity = 138/204 (67.65%), Postives = 156/204 (76.47%), Query Frame = 1


Query: 1   MASKILASSLRLARTNVSPAVSRFRPLSTAAA----VATSPSEPHKDETGISMKGVKISG 60
           MASK+LAS+LR     +S   S  R LSTAAA    +ATS  E  +D   IS+KGV+ISG
Sbjct: 1   MASKLLASNLRQTLARIS-TFSPLRSLSTAAATAPALATSVPETFEDPNEISIKGVRISG 60

Query: 61  RPIYLDMQATSPAD-------------------TRTHLYGWESDHAVETACSHVAALIGA 120
           RP+YLDMQATSP D                   +RTHLYGWESD AVETA + VA+LIGA
Sbjct: 61  RPLYLDMQATSPVDPRVLDAMLPFYLSRFGNPHSRTHLYGWESDEAVETARAQVASLIGA 120

Query: 121 SPKEIVLTSGATESNNISIKGVMNFYREKKRHLITIQTEHKCVLDSCRHLQQVGFEITYL 180
           SPKEIV TSGATESNNIS+KGV++FYR+KKRH+IT QTEHKCVLDSCRHLQQ GFE+TYL
Sbjct: 121 SPKEIVFTSGATESNNISVKGVLHFYRDKKRHVITTQTEHKCVLDSCRHLQQEGFEVTYL 180

Query: 181 PVGSDGIVDLDKLRSAIRPDTGLV 182
           PVGSDG+VDLDKLRSAIRPDTGLV
Sbjct: 181 PVGSDGLVDLDKLRSAIRPDTGLV 203

The following BLAST results are available for this feature:

BLAST of CmaCh03G008760 vs. Swiss-Prot


Match Name	E-value	Identity	Description
MNIF1_ARATH	8.7e-56	56.10	Cysteine desulfurase, mitochondrial OS=Arabidopsis thaliana GN=NIFS1 PE=1 SV=1	[more]
NFS1_ARTBC	5.5e-42	49.74	Cysteine desulfurase, mitochondrial OS=Arthroderma benhamiae (strain ATCC MYA-46...	[more]
NFS1_DROME	1.2e-41	48.24	Probable cysteine desulfurase, mitochondrial OS=Drosophila melanogaster GN=CG122...	[more]
NFS1_HUMAN	2.7e-41	56.46	Cysteine desulfurase, mitochondrial OS=Homo sapiens GN=NFS1 PE=1 SV=3	[more]
NFS1_PONAB	2.7e-41	56.46	Cysteine desulfurase, mitochondrial OS=Pongo abelii GN=NFS1 PE=2 SV=1	[more]

BLAST of CmaCh03G008760 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KIM0_CUCSA	4.8e-77	76.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_6G497010 PE=3 SV=1	[more]
A0A059ALR2_EUCGR	1.5e-62	64.08	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_I00651 PE=3 SV=1	[more]
A0A140B487_PRUPE	4.3e-62	64.25	Cysteine desulfurase 14 OS=Prunus persica var. persica GN=NFS1 PE=2 SV=1	[more]
M5WHW3_PRUPE	4.3e-62	64.25	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005512mg PE=3 SV=1	[more]
W9RYP8_9ROSA	9.7e-62	65.38	Cysteine desulfurase 1 OS=Morus notabilis GN=L484_007761 PE=3 SV=1	[more]

BLAST of CmaCh03G008760 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G65720.1	4.9e-57	56.10	nitrogen fixation S (NIFS)-like 1	[more]

BLAST of CmaCh03G008760 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659079814\|ref\|XP_008440458.1\|	8.7e-80	78.54	PREDICTED: cysteine desulfurase 1, mitochondrial [Cucumis melo]	[more]
gi\|778718827\|ref\|XP_011657914.1\|	6.9e-77	76.70	PREDICTED: cysteine desulfurase, mitochondrial [Cucumis sativus]	[more]
gi\|1021516189\|ref\|XP_016201576.1\|	8.7e-64	64.39	PREDICTED: cysteine desulfurase, mitochondrial [Arachis ipaensis]	[more]
gi\|764517110\|ref\|XP_011466445.1\|	8.7e-64	66.83	PREDICTED: cysteine desulfurase 1, mitochondrial [Fragaria vesca subsp. vesca]	[more]
gi\|1009145637\|ref\|XP_015890440.1\|	1.1e-63	67.65	PREDICTED: cysteine desulfurase, mitochondrial [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000192	Aminotrans_V_dom
IPR015421	PyrdxlP-dep_Trfase_major
IPR015424	PyrdxlP-dep_Trfase

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006534	cysteine metabolic process
biological_process	GO:0044571	[2Fe-2S] cluster assembly
cellular_component	GO:0005575	cellular_component
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0031071	cysteine desulfurase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh03G008760.1	CmaCh03G008760.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000192	Aminotransferase class V domain	PFAM	PF00266	Aminotran_5	coord: 72..182 score: 5.6
IPR015421	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	GENE3D	G3DSA:3.40.640.10		coord: 71..182 score: 3.3
IPR015424	Pyridoxal phosphate-dependent transferase	unknown	SSF53383	PLP-dependent transferases	coord: 56..182 score: 1.99
None	No IPR available	PANTHER	PTHR11601	CYSTEINE DESULFURYLASE	coord: 20..181 score: 2.8
None	No IPR available	PANTHER	PTHR11601:SF34	CYSTEINE DESULFURASE, MITOCHONDRIAL	coord: 20..181 score: 2.8

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh03G008760	Cucsa.103520	Cucumber (Gy14) v1	cgycmaB0231
CmaCh03G008760	Cla014063	Watermelon (97103) v1	cmawmB632
CmaCh03G008760	Csa6G497010	Cucumber (Chinese Long) v2	cmacuB681
CmaCh03G008760	MELO3C007668	Melon (DHL92) v3.5.1	cmameB617
CmaCh03G008760	ClCG01G017940	Watermelon (Charleston Gray)	cmawcgB579
CmaCh03G008760	CSPI06G29260	Wild cucumber (PI 183967)	cmacpiB689
CmaCh03G008760	CmoCh07G006220	Cucurbita moschata (Rifu)	cmacmoB667
CmaCh03G008760	CmoCh03G009160	Cucurbita moschata (Rifu)	cmacmoB653
CmaCh03G008760	Lsi01G008530	Bottle gourd (USVL1VR-Ls)	cmalsiB597
CmaCh03G008760	Cp4.1LG10g01770	Cucurbita pepo (Zucchini)	cmacpeB656
CmaCh03G008760	Cp4.1LG19g10330	Cucurbita pepo (Zucchini)	cmacpeB671
CmaCh03G008760	MELO3C007668.2	Melon (DHL92) v3.6.1	cmamedB703
CmaCh03G008760	CsaV3_6G044470	Cucumber (Chinese Long) v3	cmacucB0809
CmaCh03G008760	Cla97C01G017170	Watermelon (97103) v2	cmawmbB670
CmaCh03G008760	Bhi03G000832	Wax gourd	cmawgoB0798
CmaCh03G008760	CsGy6G028810	Cucumber (Gy14) v2	cgybcmaB831
CmaCh03G008760	Carg00759	Silver-seed gourd	carcmaB0567
CmaCh03G008760	Carg18517	Silver-seed gourd	carcmaB0003

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh03G008760	CmaCh07G006180	Cucurbita maxima (Rimu)	cmacmaB523

The following block(s) are covering this gene:

None