CmaCh02G016220 (gene) Cucurbita maxima (Rimu)

NameCmaCh02G016220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A
LocationCma_Chr02 : 9200374 .. 9201995 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGACTATTTGGGCCTCTAGTTACTGGTCTTTCTTCAGTAGTCCGCGCCCGAACCCGAAGTAGCTCCCGCCTCTCCAAGTCGCTGATCGCACGACGCTCTCTCTGTCGCTGATCGCACAACCCTCTCTCTGTCGCAGTTCTCATCCTCTTTCTCCGCAGTCGCCAACTCCACGATTTTCCTCAGAGTAATTCAATTCTTTCTCTCTTATCTTCATTAATATGGTTCCCAATTGATTCATCAATTTTGATTGTATAATTTTGTTGAAATCATCAAGATCTACAGAGATTAACTTCGTATTTTCCCTTCAGAATTCAGAAACGAACCCTGAGATTTGTGTCTGTTATTATTTCCTGCATTTTCTTAATTGGTATCCCAGTCGGCAACACCATTGACTGTTTAGCAACGCCGTTTGGTTATTTAGATTTGCATTTTAGTCGGCCAATTTTTGTTATTCCAATTTCGGAGACTCTCCGGAGTGATTTCATTCTGCGCACTATATGCTCTCTTCGTATACATAGGATAATGCGGTTAATGTGGGTCGTAATCACCACAATTTGTTACTGGTTACTTAGATTTATGCTATTGATTTTCAACTTTCATGTTATGGTTCTCTTTAAAGAGTTTTATGTTCTGATGATTTTATCTGATGGAACGCGAAGTCTGAATTAGTTTGATGACTCTGGTGAAGTCTGGAGTAGATCCGTTCTGGTTATAACACCTTGAGTACCCCCCTTAGCATGAATGCTGTCTTTTTAGTAAAATTATAATGTGAAGCGAGTTTAGTGCTGGATTGATTGGATTTTAATGTAGTTAATTTGAAATGTGTCTTCATTTCTTAGATTTGGGATTCTTCTTTTTGCTGCTGAAAAAGTGATTGCTTATTGCAATCTGCATCATGATGAAGTCTGAAGTTCTTTCTATGCAGCATCATAGATCTTTCAGTGGAATGAAAAAACTAGAGATTGTTTCTGCATATATTATATAATTGGTAGAGTTTCCTTGAGTTAAAAACTCATTCCATATATTTGTGAATTTTAGTTTATGGGCTTGATGTTGAGAGATTGATTGGAAATCTGCTAGTTTTGTTTACATGAGTTTTTCTTTTTTTCTTTTCTGTTGATAGTTTCATTGTCAAATGTCAATTTGAATACAAATTTGATTTGGGCTTTATCTCAAGATGATATTCTTTGTGTTTATATATATATATTTGCATGAAGAATTGTTGTTAATGTCAAAACTAAATGTGTTACAGATGTTTGACGATCGAGATCTGGGCTTCTTTGCAAATTTTCTGGGGATCTTTATATTTGTATTGGTAATAGCATTTCACTATGTGATGGCTGATCCAAAGTATGAGGGCAACTAAACCATCTCCTCCAATTTTCTTCTCCATGGAATGTATTGGTCGAGGTGAAGACGTTTTTGCAGATATTTTAGCAATTTTATAATAATAACTTTAAGTTAGCACTTTGTCTTTCAAAACAATGCAAGGATATTGTTAATTCTAAGCTTTTTATTGCTTAGTTGGTTCTAAGCTTTTTATCCCTTACCCATCATTTTTATTGAAATTTGTATGTGTTCTTCAATTTTCTATCAATTTAATAGGTAATTGACGTGGC

mRNA sequence

ATGAGACTATTTGGGCCTCTAGTTACTGGTCTTTCTTCAGTAGTCCGCGCCCGAACCCGAATCGCCAACTCCACGATTTTCCTCAGAATGTTTGACGATCGAGATCTGGGCTTCTTTGCAAATTTTCTGGGGATCTTTATATTTGTATTGGTAATAGCATTTCACTATGTGATGGCTGATCCAAAGTATGAGGGCAACTAAACCATCTCCTCCAATTTTCTTCTCCATGGAATGTATTGGTCGAGGTGAAGACGTTTTTGCAGATATTTTAGCAATTTTATAATAATAACTTTAAGTTAGCACTTTGTCTTTCAAAACAATGCAAGGATATTGTTAATTCTAAGCTTTTTATTGCTTAGTTGGTTCTAAGCTTTTTATCCCTTACCCATCATTTTTATTGAAATTTGTATGTGTTCTTCAATTTTCTATCAATTTAATAGGTAATTGACGTGGC

Coding sequence (CDS)

ATGAGACTATTTGGGCCTCTAGTTACTGGTCTTTCTTCAGTAGTCCGCGCCCGAACCCGAATCGCCAACTCCACGATTTTCCTCAGAATGTTTGACGATCGAGATCTGGGCTTCTTTGCAAATTTTCTGGGGATCTTTATATTTGTATTGGTAATAGCATTTCACTATGTGATGGCTGATCCAAAGTATGAGGGCAACTAA

Protein sequence

MRLFGPLVTGLSSVVRARTRIANSTIFLRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN
BLAST of CmaCh02G016220 vs. Swiss-Prot
Match: OST4A_ORYSJ (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Oryza sativa subsp. japonica GN=OST4A PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.9e-13
Identity = 34/37 (91.89%), Postives = 37/37 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          MFDD+DLGFFANFLGIFIFVLVIA+H+VMADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 37

BLAST of CmaCh02G016220 vs. Swiss-Prot
Match: OST4B_ORYSJ (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Oryza sativa subsp. japonica GN=OST4B PE=3 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 4.6e-12
Identity = 32/37 (86.49%), Postives = 36/37 (97.30%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          MFDD+DLGFFANFLGIFIFVLV+A+H+VMAD KYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVMAYHFVMADVKYEGN 37

BLAST of CmaCh02G016220 vs. Swiss-Prot
Match: OST4C_ARATH (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Arabidopsis thaliana GN=OST4C PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.0e-11
Identity = 30/35 (85.71%), Postives = 35/35 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYE 65
          MFDD+DLGFFANFLGIFIF+LVIA+H+VMADPK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFILVIAYHFVMADPKFE 35

BLAST of CmaCh02G016220 vs. Swiss-Prot
Match: OST4B_ARATH (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Arabidopsis thaliana GN=OST4B PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.3e-10
Identity = 27/35 (77.14%), Postives = 35/35 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYE 65
          MFDD+DLGFFANFLGIFIF++VIA+H+V+A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

BLAST of CmaCh02G016220 vs. Swiss-Prot
Match: OST4A_ARATH (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Arabidopsis thaliana GN=OST4A PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.7e-10
Identity = 29/35 (82.86%), Postives = 31/35 (88.57%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYE 65
          M DD+DLGF ANFLGIFIF LVIA+HYV ADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

BLAST of CmaCh02G016220 vs. TrEMBL
Match: A0A0E0EEG8_9ORYZ (Uncharacterized protein OS=Oryza meridionalis PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.5e-12
Identity = 37/51 (72.55%), Postives = 44/51 (86.27%), Query Frame = 1

Query: 16 RARTRIANSTIFLRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          R R R+++     +MFDD+DLGFFANFLGIFIFVLVIA+H+VMADPKYEGN
Sbjct: 18 RTRARLSSQAA-AKMFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 67

BLAST of CmaCh02G016220 vs. TrEMBL
Match: U5FZG4_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0010s21410g PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.2e-12
Identity = 35/38 (92.11%), Postives = 38/38 (100.00%), Query Frame = 1

Query: 29 RMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          +MFDD+DLGFFANFLGIFIFVLVIA+HYVMADPKYEGN
Sbjct: 28 KMFDDQDLGFFANFLGIFIFVLVIAYHYVMADPKYEGN 65

BLAST of CmaCh02G016220 vs. TrEMBL
Match: A0A0N7KNV7_ORYSJ (Os07g0620500 protein OS=Oryza sativa subsp. japonica GN=Os07g0620500 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.5e-12
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 1

Query: 28 LRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          L+MFDD+DLGFFANFLGIFIFVLVIA+H+VMADPKYEGN
Sbjct: 14 LQMFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 52

BLAST of CmaCh02G016220 vs. TrEMBL
Match: B8B8M1_ORYSI (Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_26901 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 5.5e-12
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 1

Query: 28 LRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          L+MFDD+DLGFFANFLGIFIFVLVIA+H+VMADPKYEGN
Sbjct: 14 LQMFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 52

BLAST of CmaCh02G016220 vs. TrEMBL
Match: A9PDB3_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 7.2e-12
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          MFDD+DLGFFANFLGIFIFVLVIA+HYVMADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAYHYVMADPKYEGN 37

BLAST of CmaCh02G016220 vs. TAIR10
Match: AT5G02502.1 (AT5G02502.1 Oligosaccaryltransferase)

HSP 1 Score: 64.7 bits (156), Expect = 2.4e-11
Identity = 27/35 (77.14%), Postives = 35/35 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYE 65
          MFDD+DLGFFANFLGIFIF++VIA+H+V+A+PK+E
Sbjct: 1  MFDDQDLGFFANFLGIFIFIMVIAYHFVVAEPKFE 35

BLAST of CmaCh02G016220 vs. TAIR10
Match: AT3G12587.1 (AT3G12587.1 Oligosaccaryltransferase)

HSP 1 Score: 63.5 bits (153), Expect = 5.5e-11
Identity = 29/35 (82.86%), Postives = 31/35 (88.57%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYE 65
          M DD+DLGF ANFLGIFIF LVIA+HYV ADPKYE
Sbjct: 1  MIDDQDLGFIANFLGIFIFALVIAYHYVTADPKYE 35

BLAST of CmaCh02G016220 vs. NCBI nr
Match: gi|297740396|emb|CBI30578.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 79.0 bits (193), Expect = 3.6e-12
Identity = 41/60 (68.33%), Postives = 45/60 (75.00%), Query Frame = 1

Query: 7  LVTGLSSVVRARTRIANSTIFLRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 66
          L   LSS V       N+ +   MFDD+DLGFFANFLGIFIFVLVIA+HYVMADPKYEGN
Sbjct: 12 LFLDLSSNVAVVYTELNNLLRSTMFDDQDLGFFANFLGIFIFVLVIAYHYVMADPKYEGN 71

BLAST of CmaCh02G016220 vs. NCBI nr
Match: gi|659132055|ref|XP_008465992.1| (PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Cucumis melo])

HSP 1 Score: 78.6 bits (192), Expect = 4.6e-12
Identity = 36/37 (97.30%), Postives = 37/37 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          MFDD+DLGFFANFLGIFIFVLVIAFHYVMADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 37

BLAST of CmaCh02G016220 vs. NCBI nr
Match: gi|566192018|ref|XP_006378723.1| (hypothetical protein POPTR_0010s21410g, partial [Populus trichocarpa])

HSP 1 Score: 78.6 bits (192), Expect = 4.6e-12
Identity = 35/38 (92.11%), Postives = 38/38 (100.00%), Query Frame = 1

Query: 29 RMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          +MFDD+DLGFFANFLGIFIFVLVIA+HYVMADPKYEGN
Sbjct: 28 KMFDDQDLGFFANFLGIFIFVLVIAYHYVMADPKYEGN 65

BLAST of CmaCh02G016220 vs. NCBI nr
Match: gi|218200034|gb|EEC82461.1| (hypothetical protein OsI_26901 [Oryza sativa Indica Group])

HSP 1 Score: 77.8 bits (190), Expect = 7.9e-12
Identity = 35/39 (89.74%), Postives = 39/39 (100.00%), Query Frame = 1

Query: 28 LRMFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          L+MFDD+DLGFFANFLGIFIFVLVIA+H+VMADPKYEGN
Sbjct: 14 LQMFDDQDLGFFANFLGIFIFVLVIAYHFVMADPKYEGN 52

BLAST of CmaCh02G016220 vs. NCBI nr
Match: gi|225440316|ref|XP_002263329.1| (PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A [Vitis vinifera])

HSP 1 Score: 77.4 bits (189), Expect = 1.0e-11
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = 1

Query: 30 MFDDRDLGFFANFLGIFIFVLVIAFHYVMADPKYEGN 67
          MFDD+DLGFFANFLGIFIFVLVIA+HYVMADPKYEGN
Sbjct: 1  MFDDQDLGFFANFLGIFIFVLVIAYHYVMADPKYEGN 37

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
OST4A_ORYSJ1.9e-1391.89Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ory... [more]
OST4B_ORYSJ4.6e-1286.49Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ory... [more]
OST4C_ARATH3.0e-1185.71Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4C OS=Ara... [more]
OST4B_ARATH4.3e-1077.14Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4B OS=Ara... [more]
OST4A_ARATH9.7e-1082.86Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4A OS=Ara... [more]
Match NameE-valueIdentityDescription
A0A0E0EEG8_9ORYZ2.5e-1272.55Uncharacterized protein OS=Oryza meridionalis PE=4 SV=1[more]
U5FZG4_POPTR3.2e-1292.11Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0010s21410g P... [more]
A0A0N7KNV7_ORYSJ5.5e-1289.74Os07g0620500 protein OS=Oryza sativa subsp. japonica GN=Os07g0620500 PE=4 SV=1[more]
B8B8M1_ORYSI5.5e-1289.74Putative uncharacterized protein OS=Oryza sativa subsp. indica GN=OsI_26901 PE=4... [more]
A9PDB3_POPTR7.2e-1294.59Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G02502.12.4e-1177.14 Oligosaccaryltransferase[more]
AT3G12587.15.5e-1182.86 Oligosaccaryltransferase[more]
Match NameE-valueIdentityDescription
gi|297740396|emb|CBI30578.3|3.6e-1268.33unnamed protein product [Vitis vinifera][more]
gi|659132055|ref|XP_008465992.1|4.6e-1297.30PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subuni... [more]
gi|566192018|ref|XP_006378723.1|4.6e-1292.11hypothetical protein POPTR_0010s21410g, partial [Populus trichocarpa][more]
gi|218200034|gb|EEC82461.1|7.9e-1289.74hypothetical protein OsI_26901 [Oryza sativa Indica Group][more]
gi|225440316|ref|XP_002263329.1|1.0e-1194.59PREDICTED: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subuni... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR018943Oligosaccaryltransferase
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0031410 cytoplasmic vesicle
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G016220.1CmaCh02G016220.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018943OligosaccaryltransferasePFAMPF10215Ost4coord: 30..62
score: 2.0
IPR018943OligosaccaryltransferaseunknownSSF103464Oligosaccharyltransferase subunit ost4pcoord: 30..60
score: 1.1
NoneNo IPR availablePANTHERPTHR28677FAMILY NOT NAMEDcoord: 1..66
score: 3.1

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh02G016220CmaCh19G002280Cucurbita maxima (Rimu)cmacmaB450
The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh02G016220Silver-seed gourdcarcmaB1084
CmaCh02G016220Silver-seed gourdcarcmaB1331
CmaCh02G016220Silver-seed gourdcarcmaB1340
CmaCh02G016220Cucumber (Chinese Long) v3cmacucB0717
CmaCh02G016220Cucumber (Chinese Long) v3cmacucB0718
CmaCh02G016220Watermelon (97103) v2cmawmbB611
CmaCh02G016220Watermelon (97103) v2cmawmbB662
CmaCh02G016220Cucurbita maxima (Rimu)cmacmaB344
CmaCh02G016220Cucurbita maxima (Rimu)cmacmaB504
CmaCh02G016220Cucumber (Gy14) v1cgycmaB0893
CmaCh02G016220Cucurbita moschata (Rifu)cmacmoB585
CmaCh02G016220Cucurbita moschata (Rifu)cmacmoB619
CmaCh02G016220Cucurbita moschata (Rifu)cmacmoB625
CmaCh02G016220Wild cucumber (PI 183967)cmacpiB607
CmaCh02G016220Wild cucumber (PI 183967)cmacpiB608
CmaCh02G016220Cucumber (Chinese Long) v2cmacuB601
CmaCh02G016220Cucumber (Chinese Long) v2cmacuB602
CmaCh02G016220Melon (DHL92) v3.5.1cmameB567
CmaCh02G016220Melon (DHL92) v3.5.1cmameB578
CmaCh02G016220Watermelon (Charleston Gray)cmawcgB509
CmaCh02G016220Watermelon (Charleston Gray)cmawcgB515
CmaCh02G016220Watermelon (97103) v1cmawmB546
CmaCh02G016220Watermelon (97103) v1cmawmB547
CmaCh02G016220Watermelon (97103) v1cmawmB551
CmaCh02G016220Cucurbita pepo (Zucchini)cmacpeB607
CmaCh02G016220Cucurbita pepo (Zucchini)cmacpeB615
CmaCh02G016220Cucurbita pepo (Zucchini)cmacpeB647
CmaCh02G016220Cucurbita pepo (Zucchini)cmacpeB648
CmaCh02G016220Bottle gourd (USVL1VR-Ls)cmalsiB591
CmaCh02G016220Cucumber (Gy14) v2cgybcmaB397
CmaCh02G016220Cucumber (Gy14) v2cgybcmaB398
CmaCh02G016220Melon (DHL92) v3.6.1cmamedB663