CmaCh02G012700 (gene) Cucurbita maxima (Rimu)

NameCmaCh02G012700
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionSubtilisin serine endopeptidase family protein, putative
LocationCma_Chr02 : 7445329 .. 7447557 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCCGACAATTTTCCCATATTGTTTTCCTTCATCGCCGCCCTTTTAGCCGCCATTTTGAGCTCTGCAAATGGGTCGGAAAGAAAAGCTCATATTGTTTACATGGGCGCAATCCAAAACAGAGCAATGGCGGAATCGACCCACCATTTGAATCTTCTTCGCTCGGTAATTGGAACCTCTTCCGTAACAGAAGGTTCTTACATTCGTAGCTATGGAAGAAGCTTTAATGGATTTGTTGCGAAACTCACCGGAGCTGAAGCAGAGCAACTCGCCGCCATGGACGGTGTGGTCTCTGTTTTCGAAAGCAAGGTATTCAAAACCCAAACGACAAGATCATGGGACTATTTGGGATTCCCTGCAAAACCCACAAGAAATCTCGCCGGTGAAACTGACGTCATAATTGGGTCAATCGACACGGGGATTTGGCCGGAATTGGAAAGCTTCAACGACGAGGGTATAGGTCCACCGCCGGCGCGGTGGAGAGGTACATGTGCCGGAGGAGGAAATTTCACGTGCAACAATAAAGTGATCGGAGCTCGTTTTTACACCTCCATGTCCGCCAGAGACAACGTCGGCCATGGCTCACACACCGCCTCCACAGCCGCAGGAAAATTAGAGAAAACGACTGGTTTTTATGGACTTGCCGGCGGAAATGCCAGGGGAGCAGTTCCTTCTTCGAGACTAGCGGTTTATAAAGTTTGCAATCCGGATTGCTTAGAAGAGAATATTCTGGCGGCGTTTGACGACGCCATCGCCGACGGCGTGGATCTTATCACGATTTCAATTGGCGGAGTCGGTGGGTTTAATTTTGAACATGACTCAATGGCAATTGGGTCTTACCACTCGATGGCTAAAGGAATCTTAACCGTCCAATCCGCCGGAAATTATGGGCCGGTGGACGGAACAGTGGGAAGTGTAGTTCCGTGGGTATTCACCGTCGCCGCCACCACCACAGACAGAACCATCGTAGACAAGGTTGTTCTCGGCGACGGAAACACCGTGAATGGGTATTCCGTCAACACCTTCGCTCCAAACCAAAACGTTCCATTAATTTACGCAACAAACGCTTCCAGAAATTGTTCATCAAAAAATGCAGAAATTTGCAGCGATGGTTGCTTGGATCCATTGTTGGTGAAAGGGAAGATCGTTCAATGTAAGGCCTTCGACGGCGCTTCCAGAGCTTTCAATGCCGGAGCCGCCGGAGCAATTGTTCTTAACGACAATGCCGCCAATGTTTCCTTCGTTCTTCCCTTCCCTGCAATCGCTTTGAAAATGGCGGATTATAAAAGTGTTGCAAATTACGCAATCTCTGCAACAAATCCCAATGTCACGATCTTTAGAAGCGTCGCTACTAAAGATCCTTACGCGCCGACTATCGCCGATTTCTCTAGCCGCGGCCCCAACATGTGGATGCTGGAGATCTTGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCTCGTTTTCTCCCATCGCGGCTCCTTCCGGCAATGTCGGAGACAAAAGATCTGTCGAATTTTCGATTCTGTCCGGCACTTCCATGTCTTGTCCCCATGTCGCCGGTGTCGCCGCTTATGTAAAGAGTTTCCATCCAAATTGGTCGCCGGCAGCGATCAAATCAGCCATCATGACGACGGCTAAACAAATCGTTCGTACGGATGGAATTTTAATTCGTGAATTTCTTTACGGGTCGGGTTTAATCGATCCAAATCGAGCAATCGAACCCGGGCTTGTTTATGAAATTTTTGAAAAAGACCACTTGAATGTGCTGTGTGCAAAGGGGTATGATTCAAAGACGATGGAAGCATTTGCGGGAAATGATAGTGTTTGTTCTAAAACTTGGACGAAATTTCTAGCTCGAGATTTGAATTACCCTGCAATGGTGGCTCATGTTCTACCGAAGAAACCGTTTGTGGTTAAGTTTCAAAGAACTGTGACTAATGTTGGAGTTGCCAACTCAACTTATAGATCAAAGATCTTGTCGTTTTCTGGAGTTAATGTTTTGAAGTCACGGGAAAAACTTAATGTGAGTGTTAAGCCTCAGGAACTATGGTTTGAGCATTTGAATGAGAAGAAGAGTTTTGTTGTGACGGTGGTCGGAGGGGCAATTTCAACTGAGATTGTTTTTTCTTCGGCATTAATATGGAGTGATTTGGACCATGAAGTTAGGAGTCCGATTGTTGTGATGATCAAACCACCACTCTCTACTATCTCAAACTAA

mRNA sequence

ATGGCGCCCGACAATTTTCCCATATTGTTTTCCTTCATCGCCGCCCTTTTAGCCGCCATTTTGAGCTCTGCAAATGGGTCGGAAAGAAAAGCTCATATTGTTTACATGGGCGCAATCCAAAACAGAGCAATGGCGGAATCGACCCACCATTTGAATCTTCTTCGCTCGGTAATTGGAACCTCTTCCGTAACAGAAGGTTCTTACATTCGTAGCTATGGAAGAAGCTTTAATGGATTTGTTGCGAAACTCACCGGAGCTGAAGCAGAGCAACTCGCCGCCATGGACGGTGTGGTCTCTGTTTTCGAAAGCAAGGTATTCAAAACCCAAACGACAAGATCATGGGACTATTTGGGATTCCCTGCAAAACCCACAAGAAATCTCGCCGGTGAAACTGACGTCATAATTGGGTCAATCGACACGGGGATTTGGCCGGAATTGGAAAGCTTCAACGACGAGGGTATAGGTCCACCGCCGGCGCGGTGGAGAGGTACATGTGCCGGAGGAGGAAATTTCACGTGCAACAATAAAGTGATCGGAGCTCGTTTTTACACCTCCATGTCCGCCAGAGACAACGTCGGCCATGGCTCACACACCGCCTCCACAGCCGCAGGAAAATTAGAGAAAACGACTGGTTTTTATGGACTTGCCGGCGGAAATGCCAGGGGAGCAGTTCCTTCTTCGAGACTAGCGGTTTATAAAGTTTGCAATCCGGATTGCTTAGAAGAGAATATTCTGGCGGCGTTTGACGACGCCATCGCCGACGGCGTGGATCTTATCACGATTTCAATTGGCGGAGTCGGTGGGTTTAATTTTGAACATGACTCAATGGCAATTGGGTCTTACCACTCGATGGCTAAAGGAATCTTAACCGTCCAATCCGCCGGAAATTATGGGCCGGTGGACGGAACAGTGGGAAGTGTAGTTCCGTGGGTATTCACCGTCGCCGCCACCACCACAGACAGAACCATCGTAGACAAGGTTGTTCTCGGCGACGGAAACACCGTGAATGGGTATTCCGTCAACACCTTCGCTCCAAACCAAAACGTTCCATTAATTTACGCAACAAACGCTTCCAGAAATTGTTCATCAAAAAATGCAGAAATTTGCAGCGATGGTTGCTTGGATCCATTGTTGGTGAAAGGGAAGATCGTTCAATGTAAGGCCTTCGACGGCGCTTCCAGAGCTTTCAATGCCGGAGCCGCCGGAGCAATTGTTCTTAACGACAATGCCGCCAATGTTTCCTTCGTTCTTCCCTTCCCTGCAATCGCTTTGAAAATGGCGGATTATAAAAGTGTTGCAAATTACGCAATCTCTGCAACAAATCCCAATGTCACGATCTTTAGAAGCGTCGCTACTAAAGATCCTTACGCGCCGACTATCGCCGATTTCTCTAGCCGCGGCCCCAACATGTGGATGCTGGAGATCTTGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCTCGTTTTCTCCCATCGCGGCTCCTTCCGGCAATGTCGGAGACAAAAGATCTGTCGAATTTTCGATTCTGTCCGGCACTTCCATGTCTTGTCCCCATGTCGCCGGTGTCGCCGCTTATGTAAAGAGTTTCCATCCAAATTGGTCGCCGGCAGCGATCAAATCAGCCATCATGACGACGGCTAAACAAATCGTTCGTACGGATGGAATTTTAATTCGTGAATTTCTTTACGGGTCGGGTTTAATCGATCCAAATCGAGCAATCGAACCCGGGCTTGTTTATGAAATTTTTGAAAAAGACCACTTGAATGTGCTGTGTGCAAAGGGGTATGATTCAAAGACGATGGAAGCATTTGCGGGAAATGATAGTGTTTGTTCTAAAACTTGGACGAAATTTCTAGCTCGAGATTTGAATTACCCTGCAATGGTGGCTCATGTTCTACCGAAGAAACCGTTTGTGGTTAAGTTTCAAAGAACTGTGACTAATGTTGGAGTTGCCAACTCAACTTATAGATCAAAGATCTTGTCGTTTTCTGGAGTTAATGTTTTGAAGTCACGGGAAAAACTTAATGTGAGTGTTAAGCCTCAGGAACTATGGTTTGAGCATTTGAATGAGAAGAAGAGTTTTGTTGTGACGGTGGTCGGAGGGGCAATTTCAACTGAGATTGTTTTTTCTTCGGCATTAATATGGAGTGATTTGGACCATGAAGTTAGGAGTCCGATTGTTGTGATGATCAAACCACCACTCTCTACTATCTCAAACTAA

Coding sequence (CDS)

ATGGCGCCCGACAATTTTCCCATATTGTTTTCCTTCATCGCCGCCCTTTTAGCCGCCATTTTGAGCTCTGCAAATGGGTCGGAAAGAAAAGCTCATATTGTTTACATGGGCGCAATCCAAAACAGAGCAATGGCGGAATCGACCCACCATTTGAATCTTCTTCGCTCGGTAATTGGAACCTCTTCCGTAACAGAAGGTTCTTACATTCGTAGCTATGGAAGAAGCTTTAATGGATTTGTTGCGAAACTCACCGGAGCTGAAGCAGAGCAACTCGCCGCCATGGACGGTGTGGTCTCTGTTTTCGAAAGCAAGGTATTCAAAACCCAAACGACAAGATCATGGGACTATTTGGGATTCCCTGCAAAACCCACAAGAAATCTCGCCGGTGAAACTGACGTCATAATTGGGTCAATCGACACGGGGATTTGGCCGGAATTGGAAAGCTTCAACGACGAGGGTATAGGTCCACCGCCGGCGCGGTGGAGAGGTACATGTGCCGGAGGAGGAAATTTCACGTGCAACAATAAAGTGATCGGAGCTCGTTTTTACACCTCCATGTCCGCCAGAGACAACGTCGGCCATGGCTCACACACCGCCTCCACAGCCGCAGGAAAATTAGAGAAAACGACTGGTTTTTATGGACTTGCCGGCGGAAATGCCAGGGGAGCAGTTCCTTCTTCGAGACTAGCGGTTTATAAAGTTTGCAATCCGGATTGCTTAGAAGAGAATATTCTGGCGGCGTTTGACGACGCCATCGCCGACGGCGTGGATCTTATCACGATTTCAATTGGCGGAGTCGGTGGGTTTAATTTTGAACATGACTCAATGGCAATTGGGTCTTACCACTCGATGGCTAAAGGAATCTTAACCGTCCAATCCGCCGGAAATTATGGGCCGGTGGACGGAACAGTGGGAAGTGTAGTTCCGTGGGTATTCACCGTCGCCGCCACCACCACAGACAGAACCATCGTAGACAAGGTTGTTCTCGGCGACGGAAACACCGTGAATGGGTATTCCGTCAACACCTTCGCTCCAAACCAAAACGTTCCATTAATTTACGCAACAAACGCTTCCAGAAATTGTTCATCAAAAAATGCAGAAATTTGCAGCGATGGTTGCTTGGATCCATTGTTGGTGAAAGGGAAGATCGTTCAATGTAAGGCCTTCGACGGCGCTTCCAGAGCTTTCAATGCCGGAGCCGCCGGAGCAATTGTTCTTAACGACAATGCCGCCAATGTTTCCTTCGTTCTTCCCTTCCCTGCAATCGCTTTGAAAATGGCGGATTATAAAAGTGTTGCAAATTACGCAATCTCTGCAACAAATCCCAATGTCACGATCTTTAGAAGCGTCGCTACTAAAGATCCTTACGCGCCGACTATCGCCGATTTCTCTAGCCGCGGCCCCAACATGTGGATGCTGGAGATCTTGAAGCCCGACATCGCCGCCCCCGGCGTCGAAATCCTCGCCTCGTTTTCTCCCATCGCGGCTCCTTCCGGCAATGTCGGAGACAAAAGATCTGTCGAATTTTCGATTCTGTCCGGCACTTCCATGTCTTGTCCCCATGTCGCCGGTGTCGCCGCTTATGTAAAGAGTTTCCATCCAAATTGGTCGCCGGCAGCGATCAAATCAGCCATCATGACGACGGCTAAACAAATCGTTCGTACGGATGGAATTTTAATTCGTGAATTTCTTTACGGGTCGGGTTTAATCGATCCAAATCGAGCAATCGAACCCGGGCTTGTTTATGAAATTTTTGAAAAAGACCACTTGAATGTGCTGTGTGCAAAGGGGTATGATTCAAAGACGATGGAAGCATTTGCGGGAAATGATAGTGTTTGTTCTAAAACTTGGACGAAATTTCTAGCTCGAGATTTGAATTACCCTGCAATGGTGGCTCATGTTCTACCGAAGAAACCGTTTGTGGTTAAGTTTCAAAGAACTGTGACTAATGTTGGAGTTGCCAACTCAACTTATAGATCAAAGATCTTGTCGTTTTCTGGAGTTAATGTTTTGAAGTCACGGGAAAAACTTAATGTGAGTGTTAAGCCTCAGGAACTATGGTTTGAGCATTTGAATGAGAAGAAGAGTTTTGTTGTGACGGTGGTCGGAGGGGCAATTTCAACTGAGATTGTTTTTTCTTCGGCATTAATATGGAGTGATTTGGACCATGAAGTTAGGAGTCCGATTGTTGTGATGATCAAACCACCACTCTCTACTATCTCAAACTAA

Protein sequence

MAPDNFPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSMSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIVVMIKPPLSTISN
BLAST of CmaCh02G012700 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 4.0e-197
Identity = 368/704 (52.27%), Postives = 477/704 (67.76%), Query Frame = 1

Query: 32  HIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQL 91
           +IVYMG +     +  +HHL++L+ ++GT + +    +RSY RSFNGF A L+ AE+++L
Sbjct: 33  YIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASH-LLVRSYKRSFNGFAANLSQAESQKL 92

Query: 92  AAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFND 151
             M  VVSVF SK  +  TTRSWD++GF  K  R    E+DVI+G ID+GIWPE ESF+D
Sbjct: 93  QNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDD 152

Query: 152 EGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSM--SARDNVGHGSHTASTAAGKLEKT 211
           EG GPPP +W+G+C GG  F CNNK+IGARFY     SARD  GHG+HTASTAAG   + 
Sbjct: 153 EGFGPPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQA 212

Query: 212 TGFYGLAGGNARGAVPSSRLAVYKVCNPDCLEENILAAFDDAIADGVDLITISIGGVGGF 271
             FYGLA G ARG VPS+R+A YKVC   C + +ILAAFDDAIADGVD+I+ISI      
Sbjct: 213 ASFYGLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVS 272

Query: 272 NFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVL 331
           N  + S+AIGS+H+M +GI+T  SAGN GP  G+V +V PW+ TVAA+ TDR  +D+VVL
Sbjct: 273 NLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVL 332

Query: 332 GDGNTVNGYSVNTFAPN-QNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKA 391
           G+G  + G SVNTF  N    P++Y  N SRNCS   A  CS GC+D  LVKGKIV C  
Sbjct: 333 GNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDD 392

Query: 392 FDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTIFR 451
           F G   A+ AGA G IV N    + +FV+PFPA +L   DYKS+ +Y  SA  P   I R
Sbjct: 393 FLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILR 452

Query: 452 SVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSG--NVGDKRS 511
           +    D  AP +  FSSRGP+  +  +LKPD++APG+EILA+FSP+A+PS   N  DKRS
Sbjct: 453 TEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRS 512

Query: 512 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYG 571
           V +S++SGTSM+CPHVAGVAAYVKSFHP+WSP+AIKSAIMTTA   +       +EF YG
Sbjct: 513 VRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATP-MNLKKNPEQEFAYG 572

Query: 572 SGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLN 631
           SG I+P +A +PGLVYE+  +D+L +LCA+G+DS T+   +G +  CS+   +   +DLN
Sbjct: 573 SGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSE---RTEVKDLN 632

Query: 632 YPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQEL 691
           YP M   V    PF V F+RTVTNVG  NSTY++        +V+  + +L +S++P+ L
Sbjct: 633 YPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKA--------SVVPLQPELQISIEPEIL 692

Query: 692 WFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIV 731
            F  L EKKSFVVT+ G  +      SS+++WSD  H VRSPIV
Sbjct: 693 RFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIV 723

BLAST of CmaCh02G012700 vs. Swiss-Prot
Match: SBT4C_ARATH (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 7.4e-191
Identity = 368/733 (50.20%), Postives = 488/733 (66.58%), Query Frame = 1

Query: 9   LFSFIAALLAAILSSANGSERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTEGS 68
           L+S++  LL + +S+    + + +IVYMG++ +RA    ++ H+++L+ V G SS+ EG 
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSI-EGR 69

Query: 69  YIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KPTR 128
            +RSY RSFNGF A+LT +E   +A ++GVVSVF +K+ +  TT SWD++G        R
Sbjct: 70  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 129

Query: 129 NLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTS 188
           NLA E+D IIG IDTGIWPE +SF+D+G GPPP +W+G C+GG NFTCNNK+IGAR YTS
Sbjct: 130 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS 189

Query: 189 MSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVC-NPDCLEENI 248
              RD  GHG+HTASTAAG   K T F+G+  G  RG VP+SR+A YKVC +  C  E +
Sbjct: 190 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 249

Query: 249 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTV 308
           L++FDDAIADGVDLITISIG      FE D +AIG++H+MAKGILTV SAGN GP   TV
Sbjct: 250 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 309

Query: 309 GSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNCS 368
             V PW+FTVAA+TT+R  + KVVLG+G T+ G SVN F    +  PL+Y  + AS  C 
Sbjct: 310 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 369

Query: 369 SKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAI 428
           +K A +C+  CL+   VKGKI+ C    G   A + GA  AI+      +V+F    PA 
Sbjct: 370 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRPDVAFTHHLPAS 429

Query: 429 ALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAA 488
            LK  D+KS+ +Y  S  +P   + ++    +  +P IA FSSRGPN   ++ILKPDI A
Sbjct: 430 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 489

Query: 489 PGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIK 548
           PGVEILA+FSP   PS +  D R V++S+ SGTSM+CPHVAGVAAYVK+F+P WSP+ I+
Sbjct: 490 PGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQ 549

Query: 549 SAIMTTAKQI-VRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSK 608
           SAIMTTA  +  +  GI   EF YG+G +DP  A+ PGLVYE+ + DH+  LC   Y SK
Sbjct: 550 SAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSK 609

Query: 609 TMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVL-PKKPFVVKFQRTVTNVGVANSTYRS 668
           T++  +G+   CSK   K L R+LNYP+M A +      F V F RT+TNVG  NSTY+S
Sbjct: 610 TLKIISGDTVKCSKK-NKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKS 669

Query: 669 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 728
           K+++  G        KL++ V P  L+F+ +NEK+SF VTV G  + +E+  S+ LIWSD
Sbjct: 670 KVVAGHG-------SKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSD 729

Query: 729 LDHEVRSPIVVMI 734
             H VRSPIVV I
Sbjct: 730 GTHNVRSPIVVYI 730

BLAST of CmaCh02G012700 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 5.9e-188
Identity = 366/740 (49.46%), Postives = 488/740 (65.95%), Query Frame = 1

Query: 6   FPILFSFIAALLAAILSSANGS--ERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSS 65
           F  LFS +  L  + +S+      +++ +IVY+G++ +R      + H+++L+ + G S 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 66  VTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA- 125
           + E   +RSY +SFNGF A+LT +E ++LA M+ VVSVF S+  K QTT SW+++G    
Sbjct: 67  I-ENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEG 126

Query: 126 -KPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 185
            K  R  + E+D IIG ID+GI+PE +SF+D+G GPPP +W+GTCAGG NFTCNNKVIGA
Sbjct: 127 IKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGA 186

Query: 186 RFYTSMS-----ARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVC 245
           R YT+ S     ARD  GHG+HTAS AAG     + FYGL  G ARG VP++R+AVYKVC
Sbjct: 187 RDYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC 246

Query: 246 -NPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSA 305
            N  C  E +++AFDDAIADGVD+I+ISI       FE D +AIG++H+MA G+LTV +A
Sbjct: 247 DNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAA 306

Query: 306 GNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPN-QNVPLIY 365
           GN GP   TV S  PWVF+VAA+ T+R  + KVVLGDG  + G SVNT+  N  N PL+Y
Sbjct: 307 GNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVY 366

Query: 366 ATNASRN-CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 425
             +A+ + CS   A +C   CLD  LVKGKIV C +  G   A   GA G+IV N     
Sbjct: 367 GKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDR 426

Query: 426 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 485
            +F+  FP   L   DYKS+ +Y  S  NP  T+ +S    +  AP +A FSSRGP+  +
Sbjct: 427 -AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIV 486

Query: 486 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 545
            +ILKPDI APGVEILA++SP ++P+ +  D R V++S+LSGTSM+CPHVAGVAAYVK+F
Sbjct: 487 SDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTF 546

Query: 546 HPNWSPAAIKSAIMTTAKQI-VRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLN 605
           HP WSP+ I+SAIMTTA  +     G +  EF YGSG +DP  AI PGLVYE+ + DH+N
Sbjct: 547 HPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHIN 606

Query: 606 VLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 665
            LC   Y S  +   +G++S C+K  +K L R+LNYP M A V   KPF + FQRTVTNV
Sbjct: 607 FLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 666

Query: 666 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 725
           G+  STY +K++ F G        KL++ V P+ L  + +NEK+SF+VTV   +I T+  
Sbjct: 667 GMQKSTYNAKVVKFPG-------SKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 726

Query: 726 FSSALIWSDLDHEVRSPIVV 732
            S+ LIWSD  H VRSPI+V
Sbjct: 727 VSANLIWSDGTHNVRSPIIV 737

BLAST of CmaCh02G012700 vs. Swiss-Prot
Match: SBT45_ARATH (Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana GN=SBT4.5 PE=3 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.9e-186
Identity = 372/740 (50.27%), Postives = 476/740 (64.32%), Query Frame = 1

Query: 9   LFSFIAALLAAILSSAN--GSERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTE 68
           L S I ALL    +SA+    +++ +IVYMGA+  R      +HH ++L+ V G SS+ E
Sbjct: 10  LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSI-E 69

Query: 69  GSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KP 128
              +R+Y RSFNGF A+LT +E E LA+MD VVSVF +K  K QTT SW+++G     + 
Sbjct: 70  DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129

Query: 129 TRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFY 188
            RN   E+D IIG ID+GI+PE +SF+ +G GPPP +W+G C GG NFT NNK+IGAR+Y
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189

Query: 189 TSM------SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNP 248
           T        SARD +GHGSHTASTAAG   K   FYGL  G ARG VP++R+AVYKVC+P
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249

Query: 249 D---CLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSA 308
               C  + ILAAFDDAIAD VD+ITISIGG     FE D +AIG++H+MAKGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309

Query: 309 GNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPN-QNVPLIY 368
           GN GP   TV S+ PW+FTVAA+ T+R  V KVVLG+G TV G SVN+F  N +  PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369

Query: 369 ATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANV 428
             +AS +C + +A  CS GCLD   VKGKIV C +      A   GA  +IV   +  +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIV-RSHRTDV 429

Query: 429 SFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWML 488
           + +  FP   L   DY +V +Y  S  NP   + +S    +  AP +A + SRGPN  + 
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489

Query: 489 EILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFH 548
           +ILKPDI APG EI+A++SP A PS  + D R V++S+ +GTSMSCPHVAGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549

Query: 549 PNWSPAAIKSAIMTTAKQIVRTDGIL--IREFLYGSGLIDPNRAIEPGLVYEIFEKDHLN 608
           P WSP+ I+SAIMTTA  +  +      + EF YG+G +DP  AI PGLVYE  + DH+ 
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609

Query: 609 VLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 668
            LC   Y +K +   +G+ S C+K  TK L R+LNYP+M A V   KPF V F+RTVTNV
Sbjct: 610 FLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNV 669

Query: 669 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 728
           G  N+TY++K++            KL V V P  L  + L EKKSF VT  G     E +
Sbjct: 670 GRPNATYKAKVVG----------SKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENL 729

Query: 729 FSSALIWSDLDHEVRSPIVV 732
            S+ LIWSD  H VRSPIVV
Sbjct: 730 VSAQLIWSDGVHFVRSPIVV 734

BLAST of CmaCh02G012700 vs. Swiss-Prot
Match: SBT4D_ARATH (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.9e-186
Identity = 362/731 (49.52%), Postives = 481/731 (65.80%), Query Frame = 1

Query: 11  SFIAALLAAILSSANG--SERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTEGS 70
           S ++ LL   LSS +    +++ +IVYMG++ +RA    ++ H+N+L+ V G SS+ EG 
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSI-EGR 68

Query: 71  YIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KPTR 130
            +RSY RSFNGF A+LT +E E++A M GVVSVF +K  + QTT SWD++G     K  R
Sbjct: 69  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128

Query: 131 NLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTS 190
           N   E+D IIG ID+GI PE +SF+D+G GPPP +W+G C+GG NFTCNNK+IGAR YTS
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 191 MSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNP-DCLEENI 250
              RD  GHG+HTASTAAG       F+G+  G  RG VP+SR+A YKVC P  C  E +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248

Query: 251 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTV 310
           L+AFDDAIADGVDLITISIG      F++D +AIG++H+MAKG+LTV SAGN GP   +V
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308

Query: 311 GSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNCS 370
             V PW+ TVAA+TT+R  V KVVLG+G T+ G SVN +    ++ PL+Y  + AS  C 
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368

Query: 371 SKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAI 430
           +++A +C   C+D   VKGKI+ C    G     + GA G ++      +V+F+ P PA 
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVG-LIYRTPKPDVAFIHPLPAA 428

Query: 431 ALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAA 490
            L   D++S+ +Y  S  +P   + ++ A  +  +P IA FSSRGPN   ++ILKPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488

Query: 491 PGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIK 550
           PGVEILA++SP   PS +  D R V++S+LSGTSMSCPHVAGVAAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548

Query: 551 SAIMTTAKQIVRT-DGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSK 610
           SAIMTTA  +  T  GI   EF YGSG +DP  A  PGLVYE+ + DH+  LC   Y S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608

Query: 611 TMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPK-KPFVVKFQRTVTNVGVANSTYRS 670
            ++  +G    CS+   K L R+LNYP+M A +      F V F RT+TNVG  NSTY S
Sbjct: 609 VLKVISGETVTCSEA-KKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668

Query: 671 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 730
           K+++  G        KL+V + P  L F+ +NEK+SF VTV G  + +E+  S+ LIWSD
Sbjct: 669 KVVAGHG-------SKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727

Query: 731 LDHEVRSPIVV 732
             H VRSPIVV
Sbjct: 729 GTHNVRSPIVV 727

BLAST of CmaCh02G012700 vs. TrEMBL
Match: B9HPB8_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0009s04290g PE=4 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 3.7e-205
Identity = 376/708 (53.11%), Postives = 490/708 (69.21%), Query Frame = 1

Query: 29  RKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEA 88
           ++ +IVYMG++     + S+HHL+LL+ V+  SS +E   +RSY RSFNGF AKLT  EA
Sbjct: 4   KQEYIVYMGSLPEGEYSPSSHHLSLLQEVVKDSS-SENVLVRSYKRSFNGFSAKLTSEEA 63

Query: 89  EQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELES 148
           ++L +   VVS+F S   + QTTRSWD++GF    +      +D+I+G IDTGIWPE ES
Sbjct: 64  QKLVSKKEVVSIFPSTTLQLQTTRSWDFMGFNVTASGKRGTHSDIIVGVIDTGIWPESES 123

Query: 149 FNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSMSARDNVGHGSHTASTAAGKLEK 208
           FND+G GPPP +WRG C GG NFTCNNK+IGAR Y+  SARD++GHGSHTASTAAG + K
Sbjct: 124 FNDDGFGPPPRKWRGACEGGENFTCNNKIIGARHYSFSSARDDLGHGSHTASTAAGNIVK 183

Query: 209 TTGFYGLAGGNARGAVPSSRLAVYKVCNP-DCLEENILAAFDDAIADGVDLITISIGGVG 268
              FYGLA G ARG VPS+R++ YKVC P  C   +IL+AFDDAIADGVD+ITISIGG  
Sbjct: 184 KASFYGLAQGTARGGVPSARISAYKVCGPGSCQSSDILSAFDDAIADGVDIITISIGGNQ 243

Query: 269 GFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKV 328
              F+ D +AIG +HSMAKGILT+QSAGN GPV G+V SV PW+FTVAA++TDR I+DKV
Sbjct: 244 AQEFDTDVIAIGGFHSMAKGILTLQSAGNDGPVSGSVASVAPWIFTVAASSTDRRIIDKV 303

Query: 329 VLGDGNTVNGYSVNTFA-PNQNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQC 388
           VLG+G T+ G SVN+F+   +  PL+Y   ASR C    A +C  GCLD  LVKGKIV C
Sbjct: 304 VLGNGKTLVGNSVNSFSLKGKKFPLVYGKGASRECKHLEASLCYSGCLDRTLVKGKIVLC 363

Query: 389 KAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTI 448
              +G + A  AGA GAI L  +  ++SF+LP P ++L      +V +Y  S   P+  I
Sbjct: 364 DDVNGRTEAKRAGALGAI-LPISFEDISFILPLPGLSLTEDKLNAVKSYLNSTKKPSANI 423

Query: 449 FRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRS 508
            +S A KD  AP +A FSSRGPN  + +ILKPD +APGV+ILA+F P+ +P+ +  DKR 
Sbjct: 424 LKSEAIKDNAAPEVASFSSRGPNPIISDILKPDASAPGVDILAAFPPVLSPTDDTADKRH 483

Query: 509 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTA---KQIVRTDGILIREF 568
           V++S++SGTSM+CPH AGVAA+VK+ HP+WS +AIKSAIMTTA       R++G    EF
Sbjct: 484 VKYSVMSGTSMACPHAAGVAAHVKAAHPDWSASAIKSAIMTTAWPMNVTERSEG----EF 543

Query: 569 LYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLAR 628
            +GSG ++P  AI PGLVYE  + D++ + C  GY ++ +   +G++S CSK     L R
Sbjct: 544 AFGSGHVNPVTAIHPGLVYETQKSDYIQLFCGLGYTAEKIRQISGDNSSCSKAARNTLPR 603

Query: 629 DLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKP 688
           DLNYP+M A V  ++ F +KF RTVTNVG ANSTY++KI          SR  L + V P
Sbjct: 604 DLNYPSMAAKVAVEESFTIKFHRTVTNVGNANSTYKAKIF---------SRSSLKIKVVP 663

Query: 689 QELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIVV 732
           + L F+ L EKKSF VT+VG  ++   + S++L+WSD  H VRSPIVV
Sbjct: 664 EALSFKSLKEKKSFAVTIVGRDLTYNSILSASLVWSDGSHSVRSPIVV 696

BLAST of CmaCh02G012700 vs. TrEMBL
Match: A0A067E600_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g044745mg PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 7.7e-203
Identity = 388/764 (50.79%), Postives = 513/764 (67.15%), Query Frame = 1

Query: 1   MAPDNFPILFSFIAALLAAILSSANGS-----ERKA-----------------HIVYMGA 60
           MA + F +LF+F++ +L   +S   G+     +RKA                 HIVY+G+
Sbjct: 1   MAKNGF-LLFNFLSFILFLPMSILGGAITSQDDRKASMDICFSALVVLNFLMVHIVYLGS 60

Query: 61  IQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVV 120
           +       S+ H ++L+ VIG SSV E   +RSY RSFNGF AKLT  E ++LA+M+GVV
Sbjct: 61  LFRGEYETSSQHQSILQEVIGDSSV-ENVLVRSYKRSFNGFAAKLTDHERQKLASMEGVV 120

Query: 121 SVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPP 180
           SVF S+  +  TTRSWD++G     TR  + E+D+I+G ID+GIWPE ESF+DEG GP P
Sbjct: 121 SVFPSRTLQLHTTRSWDFMGLNQSITRKRSVESDIIVGVIDSGIWPESESFSDEGFGPAP 180

Query: 181 ARWRGTCAGGGNFTCNNKVIGARFYTS-----MSARDNVGHGSHTASTAAGKLEKTTGFY 240
            +W+G C GG NFTCNNK+IGAR+YT+      +ARD  GHG+HTASTA+G   K   F+
Sbjct: 181 KKWKGACKGGRNFTCNNKIIGARYYTTDDISGNTARDIQGHGTHTASTASGNEVKDASFF 240

Query: 241 GLAGGNARGAVPSSRLAVYKVCNPD--CLEENILAAFDDAIADGVDLITISIGGVGGFNF 300
           G+  G ARG VPS+R+A YKVC+P+  C E  IL AFDDAIADGVD+ITIS+GG    NF
Sbjct: 241 GVGQGTARGGVPSARIAAYKVCSPELGCAETAILGAFDDAIADGVDIITISLGGQNTLNF 300

Query: 301 EHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGD 360
             D +AIGS+H+MAKG+LT+ SAGN GP  G+  SV PW+ +VAA+ TDR  VDKVVLG 
Sbjct: 301 TQDVIAIGSFHAMAKGVLTLHSAGNSGPFIGSTVSVAPWLMSVAASNTDRLFVDKVVLGS 360

Query: 361 GNTVNGYSVNTF-APNQNVPLIYATNASRNCSSK-NAEICSD--GCLDPLLVKGKIVQCK 420
           G T+ GYS+N+F +  +  PL+   + SR C S  + ++C+D  GC+D  L KGKIV C+
Sbjct: 361 GQTLVGYSINSFSSKGKTFPLVDGMDVSRPCESDFDPQLCTDGQGCIDSRLAKGKIVICQ 420

Query: 421 AFDGASRAFNAGAAGAIVLNDNAAN-VSFVLPFPAIALKMADYKSVANYAISATNPNVTI 480
           +FDG +    AGA G++ LND   N VS V+  PA+AL   ++ S+ +Y  S   P   I
Sbjct: 421 SFDGFNEVHKAGAEGSVSLNDVEFNKVSSVVSLPAVALNEDNFNSIYSYLKSTKKPEANI 480

Query: 481 FRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRS 540
             + A KD  AP +ADFSSRGPN  + +ILKPDI+APGV+ILA+FSP+ A S +  DKR 
Sbjct: 481 LSTEAVKDSEAPVVADFSSRGPNEIVPDILKPDISAPGVDILAAFSPLGAVSDDPEDKRQ 540

Query: 541 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYG 600
            +F+++SGTSMSCPH AGVAAYVKSFHP+WSP+AIKSAIMTTA   + +      EF +G
Sbjct: 541 AKFNVVSGTSMSCPHAAGVAAYVKSFHPDWSPSAIKSAIMTTAWP-MNSSKNKDAEFAFG 600

Query: 601 SGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLN 660
           SG I+P  A+ PGLVYE FE+D++ +LC+ GYD + +   +GN S C K   K   +DLN
Sbjct: 601 SGHINPVEAVNPGLVYETFEQDYIIMLCSMGYDERNIGKISGNISTCPKGSDKATPKDLN 660

Query: 661 YPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQEL 720
           YP+M A V P K F + F RTVTNVG+ANSTY++KIL  S +        +++ V P+ L
Sbjct: 661 YPSMAAQVSPGKSFTINFPRTVTNVGLANSTYKAKILQNSKI--------VSIKVVPESL 720

Query: 721 WFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIV 731
            F+ LNEKKSF VTV G  +    + S++L+WSD +H VRSPIV
Sbjct: 721 SFKSLNEKKSFSVTVTGKGLPNGAIVSTSLMWSDGNHRVRSPIV 753

BLAST of CmaCh02G012700 vs. TrEMBL
Match: A0A061GWL7_THECC (Subtilase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_041957 PE=4 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 8.5e-202
Identity = 389/734 (53.00%), Postives = 497/734 (67.71%), Query Frame = 1

Query: 11  SFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIR 70
           SF+  L  ++   A   +RK +I Y+G++  R  + S+HH ++L++VI  SSV     IR
Sbjct: 13  SFVLMLSMSLSPGATDEDRKVYIAYLGSLPERDYSPSSHHFSMLQAVIKQSSVAN-YLIR 72

Query: 71  SYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGE 130
           SY RSFNGF AKLT  EA +LA+M  VVS+F +KV+  QTTRSWD+LG      RN   E
Sbjct: 73  SYKRSFNGFAAKLTNEEANKLASMKEVVSIFPNKVYHLQTTRSWDFLGLKDSVKRNPTVE 132

Query: 131 TDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSM---- 190
           +DVIIG ID+GIWPE ESF+D+G GP P +W+G+C+GG NFTCNNK+IGARFY S     
Sbjct: 133 SDVIIGVIDSGIWPESESFSDKGFGPAPKKWKGSCSGGKNFTCNNKLIGARFYNSEEPRE 192

Query: 191 -SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPD-CLEENI 250
            SARD  GHG+HTASTAAG   +   F+GLA G ARG VPS+R+A YKVC  + C   +I
Sbjct: 193 ESARDGDGHGTHTASTAAGNNVEDASFFGLAQGTARGGVPSARIAAYKVCKQNGCASADI 252

Query: 251 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGT- 310
           LAAFDDAIADGVDLITIS+G     +F  DS+AIG++H+  KGILTVQSAGN G +    
Sbjct: 253 LAAFDDAIADGVDLITISVGSTTRSDFYQDSIAIGAFHAAEKGILTVQSAGNEGRLGKQG 312

Query: 311 VGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNC 370
           V SVVPW+ TVAA++ DR    KVVLG+G T+NG S+N+F       PL+Y    A+  C
Sbjct: 313 VTSVVPWILTVAASSIDRRFFSKVVLGNGKTLNGLSINSFDLKKTKFPLVYGKEIANLGC 372

Query: 371 SSK-NAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 430
           + +  A +C  GCL+  LVK KIV C  F G + A +AGAAG+I L     +VSFVLP  
Sbjct: 373 NEEITARVCETGCLNSTLVKEKIVLCDQFRGNNEARDAGAAGSI-LKTEIDDVSFVLPLA 432

Query: 431 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 490
           A AL   +Y+SV +Y  S   P   I RS   +D  AP +A FSSRGPN    +I+KPD+
Sbjct: 433 ASALSTDNYESVKSYLNSTKRPVAEILRSETIEDSAAPVVAPFSSRGPNFMEPDIMKPDL 492

Query: 491 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 550
           +APGV+ILA+FSPI +PSGN  DKR V++SILSGTSMSCPH A VAAYVK+FHP WSP+A
Sbjct: 493 SAPGVDILAAFSPIGSPSGNPADKRQVKYSILSGTSMSCPHAAAVAAYVKTFHPEWSPSA 552

Query: 551 IKSAIMTTA---KQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKG 610
           I+SA+MTTA    Q    DG    E  YGSG ++P +A +PGLVYE+ + D++  LC+ G
Sbjct: 553 IQSALMTTAFPMDQSTNPDG----ELAYGSGHVNPVKATDPGLVYEVVKGDYIKFLCSIG 612

Query: 611 YDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANST 670
           YDS+ +   +G++S CSKT    L RDLNYP++ A +LP K F V F RTVTNVG+A+ST
Sbjct: 613 YDSEKLRRISGDNSTCSKTSENILPRDLNYPSLTAQILPDKSFTVGFHRTVTNVGIASST 672

Query: 671 YRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALI 730
           Y++K+          S  KL V V P+ L F+ L EKKSF VTV G A+S   + S++L 
Sbjct: 673 YKAKV---------SSNSKLEVKVDPEVLSFKALKEKKSFNVTVTGDALSLFSMVSASLE 731

Query: 731 WSDLDHEVRSPIVV 732
           WSD  H V+SPIV+
Sbjct: 733 WSDGTHSVKSPIVI 731

BLAST of CmaCh02G012700 vs. TrEMBL
Match: A0A061GXN7_THECC (Subtilase 4.13, putative isoform 2 OS=Theobroma cacao GN=TCM_041957 PE=4 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.4e-201
Identity = 389/734 (53.00%), Postives = 497/734 (67.71%), Query Frame = 1

Query: 11  SFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIR 70
           SF+  L  ++   A   +RK +I Y+G++  R  + S+HH ++L++VI  SSV     IR
Sbjct: 6   SFVLMLSMSLSPGAADEDRKVYIAYLGSLPERDYSPSSHHFSMLQAVIKQSSVAN-YLIR 65

Query: 71  SYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGE 130
           SY RSFNGF AKLT  EA +LA+M  VVS+F +KV+  QTTRSWD+LG      RN   E
Sbjct: 66  SYKRSFNGFAAKLTNEEANKLASMKEVVSIFPNKVYHLQTTRSWDFLGLKDSVKRNPTVE 125

Query: 131 TDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSM---- 190
           +DVIIG ID+GIWPE ESF+D+G GP P +W+G+C+GG NFTCNNK+IGARFY S     
Sbjct: 126 SDVIIGVIDSGIWPESESFSDKGFGPAPKKWKGSCSGGKNFTCNNKLIGARFYNSEEPRE 185

Query: 191 -SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPD-CLEENI 250
            SARD  GHG+HTASTAAG   +   F+GLA G ARG VPS+R+A YKVC  + C   +I
Sbjct: 186 ESARDGDGHGTHTASTAAGNNVEDASFFGLAQGTARGGVPSARIAAYKVCKQNGCASADI 245

Query: 251 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGT- 310
           LAAFDDAIADGVDLITIS+G     +F  DS+AIG++H+  KGILTVQSAGN G +    
Sbjct: 246 LAAFDDAIADGVDLITISVGSTTRSDFYQDSIAIGAFHAAEKGILTVQSAGNEGRLGKQG 305

Query: 311 VGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNC 370
           V SVVPW+ TVAA++ DR    KVVLG+G T+NG S+N+F       PL+Y    A+  C
Sbjct: 306 VTSVVPWILTVAASSIDRRFFSKVVLGNGKTLNGLSINSFDLKKTKFPLVYGKEIANLGC 365

Query: 371 SSK-NAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFP 430
           + +  A +C  GCL+  LVK KIV C  F G + A +AGAAG+I L     +VSFVLP  
Sbjct: 366 NEEITARVCETGCLNSTLVKEKIVLCDQFRGNNEARDAGAAGSI-LKTEIDDVSFVLPLA 425

Query: 431 AIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDI 490
           A AL   +Y+SV +Y  S   P   I RS   +D  AP +A FSSRGPN    +I+KPD+
Sbjct: 426 ASALSTDNYESVKSYLNSTKRPVAEILRSETIEDSAAPVVAPFSSRGPNFMEPDIMKPDL 485

Query: 491 AAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAA 550
           +APGV+ILA+FSPI +PSGN  DKR V++SILSGTSMSCPH A VAAYVK+FHP WSP+A
Sbjct: 486 SAPGVDILAAFSPIGSPSGNPADKRQVKYSILSGTSMSCPHAAAVAAYVKTFHPEWSPSA 545

Query: 551 IKSAIMTTA---KQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKG 610
           I+SA+MTTA    Q    DG    E  YGSG ++P +A +PGLVYE+ + D++  LC+ G
Sbjct: 546 IQSALMTTAFPMDQSTNPDG----ELAYGSGHVNPVKATDPGLVYEVVKGDYIKFLCSIG 605

Query: 611 YDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANST 670
           YDS+ +   +G++S CSKT    L RDLNYP++ A +LP K F V F RTVTNVG+A+ST
Sbjct: 606 YDSEKLRRISGDNSTCSKTSENILPRDLNYPSLTAQILPDKSFTVGFHRTVTNVGIASST 665

Query: 671 YRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALI 730
           Y++K+          S  KL V V P+ L F+ L EKKSF VTV G A+S   + S++L 
Sbjct: 666 YKAKV---------SSNSKLEVKVDPEVLSFKALKEKKSFNVTVTGDALSLFSMVSASLE 724

Query: 731 WSDLDHEVRSPIVV 732
           WSD  H V+SPIV+
Sbjct: 726 WSDGTHSVKSPIVI 724

BLAST of CmaCh02G012700 vs. TrEMBL
Match: W9RUC3_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_025323 PE=4 SV=1)

HSP 1 Score: 709.9 bits (1831), Expect = 3.2e-201
Identity = 381/735 (51.84%), Postives = 497/735 (67.62%), Query Frame = 1

Query: 6   FPILFS-FIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVT 65
           F + F+ FI AL  +    A   +RKA+IVYMGA+ +     STHH ++L+S+I  SS+ 
Sbjct: 9   FNVFFTVFILAL--SFSCKAIDEDRKAYIVYMGALPDGVYTPSTHHFSILQSIIQDSSIA 68

Query: 66  EGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPT 125
            GS+IRSY RSFNGF AKLT  E ++LA+M  +VS+F S++F+TQTTRSWD++G   K  
Sbjct: 69  -GSFIRSYKRSFNGFAAKLTDQERQKLASMKEIVSIFRSEIFQTQTTRSWDFMGLHEKID 128

Query: 126 RNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYT 185
           R    E+D IIG IDTGIWPELESF+DEG GPPP +W+G C GG NFTCNNK++ AR+Y 
Sbjct: 129 RKPTAESDTIIGVIDTGIWPELESFSDEGFGPPPKKWKGACTGGTNFTCNNKIVRARYYL 188

Query: 186 SM-----SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPD- 245
           S      SARD VGHGSHTASTAAG   K   FYG+A G ARG VPSSR+A YKVC+P+ 
Sbjct: 189 SFGSGYESARDLVGHGSHTASTAAGNNIKDVSFYGIAEGTARGGVPSSRIAAYKVCDPEG 248

Query: 246 CLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYG 305
           C   +ILAAFDDAIADGVD+IT+S+G  G   FE D +AIGS+H+MAKGILT+ SAGN G
Sbjct: 249 CYSHSILAAFDDAIADGVDIITVSLGSSGAHAFEVDPIAIGSFHAMAKGILTLNSAGNSG 308

Query: 306 PVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPNQNV-PLIYATNA 365
           P   TV S+ PW+ TVAA++ DR I+ KV L +G T+ G SVN+F  N+   PL++  N 
Sbjct: 309 PSLQTVSSLAPWLMTVAASSIDRRIISKVSLANGTTIIGSSVNSFKLNETTFPLVHGRNG 368

Query: 366 SRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVL 425
           S  CS   A +C +G LD  LV+G IV C    G   A+ AGA G+I ++   ++VSFV 
Sbjct: 369 STRCSELRARLCEEGFLDRCLVEGTIVLCDRVIGIKEAYRAGALGSISVDGTFSDVSFVS 428

Query: 426 PFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILK 485
           P PA+ L   +Y ++ +Y  S  +P  TI +S   KD  AP +  FSSRGPN    +I+K
Sbjct: 429 PLPAVTLNQTNYMAIISYLCSTEDPRATILQSETIKDIAAPVVVSFSSRGPNAITPDIMK 488

Query: 486 PDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWS 545
           PDI APGV+ILAS+SP+  PS +  D R V+++I+SGTSMSCPHVAG AAYVK+FHP+WS
Sbjct: 489 PDITAPGVDILASYSPVVPPSDSFDDTRRVKYNIMSGTSMSCPHVAGAAAYVKTFHPDWS 548

Query: 546 PAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKG 605
           P+AIKSA+MTTA  +  TD    +EF +GSG ++P  AI PGLVYE  E D++  LC+ G
Sbjct: 549 PSAIKSALMTTAFIMNDTDH-PNKEFDHGSGHVNPTTAINPGLVYESSEVDYVAFLCSIG 608

Query: 606 YDSKTMEAFAGNDSVCSKTWTKFLA-RDLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANS 665
           YD   +   +G++S C K+  +  + R LNYP+M A V   KPF+V+F R VT VG  NS
Sbjct: 609 YDDVKVRRISGDNSTCPKSSEEGSSPRYLNYPSMTAQVPAGKPFMVRFPRRVTYVGDGNS 668

Query: 666 TYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSAL 725
           TY ++IL          + KL++ V P    F+ L E+KSF V V G  +S   + S++L
Sbjct: 669 TYTARIL---------PKLKLHIKVTPSVFSFQSLGEEKSFDVIVKGKGLSDNSIVSTSL 728

Query: 726 IWSDLDHEVRSPIVV 732
           +W+D  H VRSPIV+
Sbjct: 729 VWTDRFHNVRSPIVL 730

BLAST of CmaCh02G012700 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 683.3 bits (1762), Expect = 1.6e-196
Identity = 364/700 (52.00%), Postives = 472/700 (67.43%), Query Frame = 1

Query: 36  MGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMD 95
           MG +     +  +HHL++L+ ++GT + +    +RSY RSFNGF A L+ AE+++L  M 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASH-LLVRSYKRSFNGFAANLSQAESQKLQNMK 60

Query: 96  GVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIG 155
            VVSVF SK  +  TTRSWD++GF  K  R    E+DVI+G ID+GIWPE ESF+DEG G
Sbjct: 61  EVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFG 120

Query: 156 PPPARWRGTCAGGGNFTCNNKVIGARFYTSM--SARDNVGHGSHTASTAAGKLEKTTGFY 215
           PPP +W+G+C GG  F CNNK+IGARFY     SARD  GHG+HTASTAAG   +   FY
Sbjct: 121 PPPKKWKGSCKGGLKFACNNKLIGARFYNKFADSARDEEGHGTHTASTAAGNAVQAASFY 180

Query: 216 GLAGGNARGAVPSSRLAVYKVCNPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEH 275
           GLA G ARG VPS+R+A YKVC   C + +ILAAFDDAIADGVD+I+ISI      N  +
Sbjct: 181 GLAQGTARGGVPSARIAAYKVCFNRCNDVDILAAFDDAIADGVDVISISISADYVSNLLN 240

Query: 276 DSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGN 335
            S+AIGS+H+M +GI+T  SAGN GP  G+V +V PW+ TVAA+ TDR  +D+VVLG+G 
Sbjct: 241 ASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDRVVLGNGK 300

Query: 336 TVNGYSVNTFAPN-QNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGA 395
            + G SVNTF  N    P++Y  N SRNCS   A  CS GC+D  LVKGKIV C  F G 
Sbjct: 301 ALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVLCDDFLGY 360

Query: 396 SRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVAT 455
             A+ AGA G IV N    + +FV+PFPA +L   DYKS+ +Y  SA  P   I R+   
Sbjct: 361 REAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQAEILRTEEI 420

Query: 456 KDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSG--NVGDKRSVEFS 515
            D  AP +  FSSRGP+  +  +LKPD++APG+EILA+FSP+A+PS   N  DKRSV +S
Sbjct: 421 VDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPEDKRSVRYS 480

Query: 516 ILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLI 575
           ++SGTSM+CPHVAGVAAYVKSFHP+WSP+AIKSAIMTTA  +        +EF YGSG I
Sbjct: 481 VMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE-QEFAYGSGQI 540

Query: 576 DPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAM 635
           +P +A +PGLVYE+  +D+L +LCA+G+DS T+   +G +  CS+   +   +DLNYP M
Sbjct: 541 NPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSE---RTEVKDLNYPTM 600

Query: 636 VAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEH 695
              V    PF V F+RTVTNVG  NSTY++ ++          + +L +S++P+ L F  
Sbjct: 601 TTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPL--------QPELQISIEPEILRFGF 660

Query: 696 LNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIV 731
           L EKKSFVVT+ G  +      SS+++WSD  H VRSPIV
Sbjct: 661 LEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIV 687

BLAST of CmaCh02G012700 vs. TAIR10
Match: AT5G59090.1 (AT5G59090.1 subtilase 4.12)

HSP 1 Score: 668.7 bits (1724), Expect = 4.2e-192
Identity = 368/733 (50.20%), Postives = 488/733 (66.58%), Query Frame = 1

Query: 9   LFSFIAALLAAILSSANGSERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTEGS 68
           L+S++  LL + +S+    + + +IVYMG++ +RA    ++ H+++L+ V G SS+ EG 
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSI-EGR 69

Query: 69  YIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KPTR 128
            +RSY RSFNGF A+LT +E   +A ++GVVSVF +K+ +  TT SWD++G        R
Sbjct: 70  LVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKR 129

Query: 129 NLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTS 188
           NLA E+D IIG IDTGIWPE +SF+D+G GPPP +W+G C+GG NFTCNNK+IGAR YTS
Sbjct: 130 NLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS 189

Query: 189 MSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVC-NPDCLEENI 248
              RD  GHG+HTASTAAG   K T F+G+  G  RG VP+SR+A YKVC +  C  E +
Sbjct: 190 EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCSSEAL 249

Query: 249 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTV 308
           L++FDDAIADGVDLITISIG      FE D +AIG++H+MAKGILTV SAGN GP   TV
Sbjct: 250 LSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPKPTTV 309

Query: 309 GSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNCS 368
             V PW+FTVAA+TT+R  + KVVLG+G T+ G SVN F    +  PL+Y  + AS  C 
Sbjct: 310 SHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAASSACD 369

Query: 369 SKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAI 428
           +K A +C+  CL+   VKGKI+ C    G   A + GA  AI+      +V+F    PA 
Sbjct: 370 AKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAI-AIIDKSPRPDVAFTHHLPAS 429

Query: 429 ALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAA 488
            LK  D+KS+ +Y  S  +P   + ++    +  +P IA FSSRGPN   ++ILKPDI A
Sbjct: 430 GLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDILKPDITA 489

Query: 489 PGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIK 548
           PGVEILA+FSP   PS +  D R V++S+ SGTSM+CPHVAGVAAYVK+F+P WSP+ I+
Sbjct: 490 PGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRWSPSMIQ 549

Query: 549 SAIMTTAKQI-VRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSK 608
           SAIMTTA  +  +  GI   EF YG+G +DP  A+ PGLVYE+ + DH+  LC   Y SK
Sbjct: 550 SAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCGMNYTSK 609

Query: 609 TMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVL-PKKPFVVKFQRTVTNVGVANSTYRS 668
           T++  +G+   CSK   K L R+LNYP+M A +      F V F RT+TNVG  NSTY+S
Sbjct: 610 TLKIISGDTVKCSKK-NKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPNSTYKS 669

Query: 669 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 728
           K+++  G        KL++ V P  L+F+ +NEK+SF VTV G  + +E+  S+ LIWSD
Sbjct: 670 KVVAGHG-------SKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSD 729

Query: 729 LDHEVRSPIVVMI 734
             H VRSPIVV I
Sbjct: 730 GTHNVRSPIVVYI 730

BLAST of CmaCh02G012700 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 659.1 bits (1699), Expect = 3.3e-189
Identity = 366/740 (49.46%), Postives = 488/740 (65.95%), Query Frame = 1

Query: 6   FPILFSFIAALLAAILSSANGS--ERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSS 65
           F  LFS +  L  + +S+      +++ +IVY+G++ +R      + H+++L+ + G S 
Sbjct: 7   FIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQEITGESL 66

Query: 66  VTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA- 125
           + E   +RSY +SFNGF A+LT +E ++LA M+ VVSVF S+  K QTT SW+++G    
Sbjct: 67  I-ENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFMGLKEG 126

Query: 126 -KPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGA 185
            K  R  + E+D IIG ID+GI+PE +SF+D+G GPPP +W+GTCAGG NFTCNNKVIGA
Sbjct: 127 IKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNNKVIGA 186

Query: 186 RFYTSMS-----ARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVC 245
           R YT+ S     ARD  GHG+HTAS AAG     + FYGL  G ARG VP++R+AVYKVC
Sbjct: 187 RDYTAKSKANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPAARIAVYKVC 246

Query: 246 -NPDCLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSA 305
            N  C  E +++AFDDAIADGVD+I+ISI       FE D +AIG++H+MA G+LTV +A
Sbjct: 247 DNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMAVGVLTVNAA 306

Query: 306 GNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPN-QNVPLIY 365
           GN GP   TV S  PWVF+VAA+ T+R  + KVVLGDG  + G SVNT+  N  N PL+Y
Sbjct: 307 GNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDMNGTNYPLVY 366

Query: 366 ATNASRN-CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAAN 425
             +A+ + CS   A +C   CLD  LVKGKIV C +  G   A   GA G+IV N     
Sbjct: 367 GKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGSIVKNPEPDR 426

Query: 426 VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWM 485
            +F+  FP   L   DYKS+ +Y  S  NP  T+ +S    +  AP +A FSSRGP+  +
Sbjct: 427 -AFIRSFPVSFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVASFSSRGPSSIV 486

Query: 486 LEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSF 545
            +ILKPDI APGVEILA++SP ++P+ +  D R V++S+LSGTSM+CPHVAGVAAYVK+F
Sbjct: 487 SDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHVAGVAAYVKTF 546

Query: 546 HPNWSPAAIKSAIMTTAKQI-VRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLN 605
           HP WSP+ I+SAIMTTA  +     G +  EF YGSG +DP  AI PGLVYE+ + DH+N
Sbjct: 547 HPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLVYELTKADHIN 606

Query: 606 VLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 665
            LC   Y S  +   +G++S C+K  +K L R+LNYP M A V   KPF + FQRTVTNV
Sbjct: 607 FLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFNITFQRTVTNV 666

Query: 666 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 725
           G+  STY +K++ F G        KL++ V P+ L  + +NEK+SF+VTV   +I T+  
Sbjct: 667 GMQKSTYNAKVVKFPG-------SKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGTKQP 726

Query: 726 FSSALIWSDLDHEVRSPIVV 732
            S+ LIWSD  H VRSPI+V
Sbjct: 727 VSANLIWSDGTHNVRSPIIV 737

BLAST of CmaCh02G012700 vs. TAIR10
Match: AT3G46840.1 (AT3G46840.1 Subtilase family protein)

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 372/740 (50.27%), Postives = 476/740 (64.32%), Query Frame = 1

Query: 9   LFSFIAALLAAILSSAN--GSERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTE 68
           L S I ALL    +SA+    +++ +IVYMGA+  R      +HH ++L+ V G SS+ E
Sbjct: 10  LLSCIFALLVVSFASADKDDQDKQEYIVYMGALPARVDYMPMSHHTSILQDVTGESSI-E 69

Query: 69  GSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KP 128
              +R+Y RSFNGF A+LT +E E LA+MD VVSVF +K  K QTT SW+++G     + 
Sbjct: 70  DRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKLQTTTSWNFMGLKESKRT 129

Query: 129 TRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFY 188
            RN   E+D IIG ID+GI+PE +SF+ +G GPPP +W+G C GG NFT NNK+IGAR+Y
Sbjct: 130 KRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGKNFTWNNKLIGARYY 189

Query: 189 TSM------SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNP 248
           T        SARD +GHGSHTASTAAG   K   FYGL  G ARG VP++R+AVYKVC+P
Sbjct: 190 TPKLEGFPESARDYMGHGSHTASTAAGNAVKHVSFYGLGNGTARGGVPAARIAVYKVCDP 249

Query: 249 D---CLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSA 308
               C  + ILAAFDDAIAD VD+ITISIGG     FE D +AIG++H+MAKGIL V SA
Sbjct: 250 GVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEEDPIAIGAFHAMAKGILIVNSA 309

Query: 309 GNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPN-QNVPLIY 368
           GN GP   TV S+ PW+FTVAA+ T+R  V KVVLG+G TV G SVN+F  N +  PL+Y
Sbjct: 310 GNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKTV-GRSVNSFDLNGKKYPLVY 369

Query: 369 ATNASRNCSSKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANV 428
             +AS +C + +A  CS GCLD   VKGKIV C +      A   GA  +IV   +  +V
Sbjct: 370 GKSASSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQNPDEAQAMGAIASIV-RSHRTDV 429

Query: 429 SFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWML 488
           + +  FP   L   DY +V +Y  S  NP   + +S    +  AP +A + SRGPN  + 
Sbjct: 430 ASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKSETIFNQRAPVVASYFSRGPNTIIP 489

Query: 489 EILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFH 548
           +ILKPDI APG EI+A++SP A PS  + D R V++S+ +GTSMSCPHVAGVAAY+KSFH
Sbjct: 490 DILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKYSVDTGTSMSCPHVAGVAAYLKSFH 549

Query: 549 PNWSPAAIKSAIMTTAKQIVRTDGIL--IREFLYGSGLIDPNRAIEPGLVYEIFEKDHLN 608
           P WSP+ I+SAIMTTA  +  +      + EF YG+G +DP  AI PGLVYE  + DH+ 
Sbjct: 550 PRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGAGHVDPITAIHPGLVYEANKSDHIA 609

Query: 609 VLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVKFQRTVTNV 668
            LC   Y +K +   +G+ S C+K  TK L R+LNYP+M A V   KPF V F+RTVTNV
Sbjct: 610 FLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNYPSMTAQVSAAKPFKVIFRRTVTNV 669

Query: 669 GVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIV 728
           G  N+TY++K++            KL V V P  L  + L EKKSF VT  G     E +
Sbjct: 670 GRPNATYKAKVVG----------SKLKVKVVPAVLSLKSLYEKKSFTVTASGAGPKAENL 729

Query: 729 FSSALIWSDLDHEVRSPIVV 732
            S+ LIWSD  H VRSPIVV
Sbjct: 730 VSAQLIWSDGVHFVRSPIVV 734

BLAST of CmaCh02G012700 vs. TAIR10
Match: AT5G59120.1 (AT5G59120.1 subtilase 4.13)

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 362/731 (49.52%), Postives = 481/731 (65.80%), Query Frame = 1

Query: 11  SFIAALLAAILSSANG--SERKAHIVYMGAIQNRA-MAESTHHLNLLRSVIGTSSVTEGS 70
           S ++ LL   LSS +    +++ +IVYMG++ +RA    ++ H+N+L+ V G SS+ EG 
Sbjct: 9   SLLSCLLVLFLSSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTGESSI-EGR 68

Query: 71  YIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPA--KPTR 130
            +RSY RSFNGF A+LT +E E++A M GVVSVF +K  + QTT SWD++G     K  R
Sbjct: 69  LVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKR 128

Query: 131 NLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTS 190
           N   E+D IIG ID+GI PE +SF+D+G GPPP +W+G C+GG NFTCNNK+IGAR YTS
Sbjct: 129 NPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLIGARDYTS 188

Query: 191 MSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNP-DCLEENI 250
              RD  GHG+HTASTAAG       F+G+  G  RG VP+SR+A YKVC P  C  E +
Sbjct: 189 EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEAL 248

Query: 251 LAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTV 310
           L+AFDDAIADGVDLITISIG      F++D +AIG++H+MAKG+LTV SAGN GP   +V
Sbjct: 249 LSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISV 308

Query: 311 GSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQNVPLIYATN-ASRNCS 370
             V PW+ TVAA+TT+R  V KVVLG+G T+ G SVN +    ++ PL+Y  + AS  C 
Sbjct: 309 SGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSAASSACD 368

Query: 371 SKNAEICSDGCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAI 430
           +++A +C   C+D   VKGKI+ C    G     + GA G ++      +V+F+ P PA 
Sbjct: 369 AESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAVG-LIYRTPKPDVAFIHPLPAA 428

Query: 431 ALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAA 490
            L   D++S+ +Y  S  +P   + ++ A  +  +P IA FSSRGPN   ++ILKPDI A
Sbjct: 429 GLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITA 488

Query: 491 PGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIK 550
           PGVEILA++SP   PS +  D R V++S+LSGTSMSCPHVAGVAAYVK+F+P WSP+ I+
Sbjct: 489 PGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQ 548

Query: 551 SAIMTTAKQIVRT-DGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSK 610
           SAIMTTA  +  T  GI   EF YGSG +DP  A  PGLVYE+ + DH+  LC   Y S+
Sbjct: 549 SAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQ 608

Query: 611 TMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPK-KPFVVKFQRTVTNVGVANSTYRS 670
            ++  +G    CS+   K L R+LNYP+M A +      F V F RT+TNVG  NSTY S
Sbjct: 609 VLKVISGETVTCSEA-KKILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTS 668

Query: 671 KILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSD 730
           K+++  G        KL+V + P  L F+ +NEK+SF VTV G  + +E+  S+ LIWSD
Sbjct: 669 KVVAGHG-------SKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSD 727

Query: 731 LDHEVRSPIVV 732
             H VRSPIVV
Sbjct: 729 GTHNVRSPIVV 727

BLAST of CmaCh02G012700 vs. NCBI nr
Match: gi|568877765|ref|XP_006491889.1| (PREDICTED: subtilisin-like protease SBT4.8 [Citrus sinensis])

HSP 1 Score: 727.2 bits (1876), Expect = 2.8e-206
Identity = 388/748 (51.87%), Postives = 513/748 (68.58%), Query Frame = 1

Query: 1   MAPDNFPILFSFIAALLAAILSSANGS-----ERKAHIVYMGAIQNRAMAESTHHLNLLR 60
           MA + F +LF+F++ +L   +S   G+     +RK HIVY+G++       S+ H ++L+
Sbjct: 1   MAKNGF-LLFNFLSFILFLPMSILGGAITSQDDRKVHIVYLGSLSRGEYETSSQHQSILQ 60

Query: 61  SVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWD 120
            VIG SSV E   +RSY RSFNGF AKLT  E ++LA+M+GVVSVF S+  +  TTRSWD
Sbjct: 61  EVIGDSSV-ENVLVRSYKRSFNGFAAKLTDRERQKLASMEGVVSVFPSRTLQLHTTRSWD 120

Query: 121 YLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNN 180
           ++G     TR  + E+D+I+G ID+GIWPE ESF+DEG GP P +W+G C GG NFTCNN
Sbjct: 121 FMGLNQSITRKRSVESDIIVGVIDSGIWPESESFSDEGFGPAPKKWKGACKGGRNFTCNN 180

Query: 181 KVIGARFYTS-----MSARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLA 240
           K+IGAR+YT+      +ARD  GHG+HTASTA+G   K   F+G+  G ARG VPS+R+A
Sbjct: 181 KIIGARYYTTDDISGNTARDIQGHGTHTASTASGNEVKDASFFGVGQGTARGGVPSARIA 240

Query: 241 VYKVCNPD--CLEENILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGI 300
            YKVC+P+  C E  IL AFDDAIADGVD+ITIS+GG    NF  D +AIGS+H+MAKG+
Sbjct: 241 AYKVCSPELGCAETAILGAFDDAIADGVDIITISLGGQNTLNFTEDVIAIGSFHAMAKGV 300

Query: 301 LTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTF-APNQ 360
           LT+ SAGN GP  G+  SV PW+ +VAA+ TDR  VDKVVLG G T+ GYS+N+F +  +
Sbjct: 301 LTLHSAGNSGPFIGSTVSVAPWLMSVAASNTDRLFVDKVVLGSGQTLVGYSINSFSSKGK 360

Query: 361 NVPLIYATNASRNCSSK-NAEICSD--GCLDPLLVKGKIVQCKAFDGASRAFNAGAAGAI 420
             PL+   + SR C S  + ++C+D  GC+D  L KGKIV C++FDG +    AGA G++
Sbjct: 361 TFPLVDGMDVSRPCESDFDPQLCTDGQGCIDSRLAKGKIVICQSFDGFNEVHKAGAEGSV 420

Query: 421 VLNDNAAN-VSFVLPFPAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADF 480
            LND   N VS V+  PA+AL   ++ S+ +Y  S   P   I  + A KD  AP +ADF
Sbjct: 421 SLNDVEFNKVSSVVSLPAVALNEDNFNSIYSYLKSTKKPEANILSTEAVKDSEAPVVADF 480

Query: 481 SSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVA 540
           SSRGPN  + +ILKPDI+APGV+ILA+FSP+ A S +  DKR  +F+++SGTSMSCPH A
Sbjct: 481 SSRGPNEIVPDILKPDISAPGVDILAAFSPLGAVSDDPEDKRQAKFNVVSGTSMSCPHAA 540

Query: 541 GVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYE 600
           GVAAYVKSFHP+WSP+AIKSAIMTTA   + +      EF +GSG I+P  A+ PGLVYE
Sbjct: 541 GVAAYVKSFHPDWSPSAIKSAIMTTAWP-MNSSKNKDAEFAFGSGHINPVEAVNPGLVYE 600

Query: 601 IFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLNYPAMVAHVLPKKPFVVK 660
            FE+D++ +LC+ GYD + +   +GN S C K   K   +DLNYP+M A V P K F + 
Sbjct: 601 TFEQDYIIMLCSMGYDERNIGKISGNISTCPKGSDKATPKDLNYPSMAAQVSPGKSFTIN 660

Query: 661 FQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVG 720
           F RTVTNVG+ANSTY++KIL  S +        +++ V P+ L F+ LNEKKSF VTV G
Sbjct: 661 FPRTVTNVGLANSTYKAKILQNSKI--------VSIKVVPESLSFKSLNEKKSFSVTVTG 720

Query: 721 GAISTEIVFSSALIWSDLDHEVRSPIVV 732
             +    + S++L+WSD +H VRSPIVV
Sbjct: 721 KGLPNGAIVSTSLMWSDGNHRVRSPIVV 737

BLAST of CmaCh02G012700 vs. NCBI nr
Match: gi|224106379|ref|XP_002314147.1| (subtilase family protein [Populus trichocarpa])

HSP 1 Score: 723.0 bits (1865), Expect = 5.3e-205
Identity = 376/708 (53.11%), Postives = 490/708 (69.21%), Query Frame = 1

Query: 29  RKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEA 88
           ++ +IVYMG++     + S+HHL+LL+ V+  SS +E   +RSY RSFNGF AKLT  EA
Sbjct: 4   KQEYIVYMGSLPEGEYSPSSHHLSLLQEVVKDSS-SENVLVRSYKRSFNGFSAKLTSEEA 63

Query: 89  EQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELES 148
           ++L +   VVS+F S   + QTTRSWD++GF    +      +D+I+G IDTGIWPE ES
Sbjct: 64  QKLVSKKEVVSIFPSTTLQLQTTRSWDFMGFNVTASGKRGTHSDIIVGVIDTGIWPESES 123

Query: 149 FNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSMSARDNVGHGSHTASTAAGKLEK 208
           FND+G GPPP +WRG C GG NFTCNNK+IGAR Y+  SARD++GHGSHTASTAAG + K
Sbjct: 124 FNDDGFGPPPRKWRGACEGGENFTCNNKIIGARHYSFSSARDDLGHGSHTASTAAGNIVK 183

Query: 209 TTGFYGLAGGNARGAVPSSRLAVYKVCNP-DCLEENILAAFDDAIADGVDLITISIGGVG 268
              FYGLA G ARG VPS+R++ YKVC P  C   +IL+AFDDAIADGVD+ITISIGG  
Sbjct: 184 KASFYGLAQGTARGGVPSARISAYKVCGPGSCQSSDILSAFDDAIADGVDIITISIGGNQ 243

Query: 269 GFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKV 328
              F+ D +AIG +HSMAKGILT+QSAGN GPV G+V SV PW+FTVAA++TDR I+DKV
Sbjct: 244 AQEFDTDVIAIGGFHSMAKGILTLQSAGNDGPVSGSVASVAPWIFTVAASSTDRRIIDKV 303

Query: 329 VLGDGNTVNGYSVNTFA-PNQNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQC 388
           VLG+G T+ G SVN+F+   +  PL+Y   ASR C    A +C  GCLD  LVKGKIV C
Sbjct: 304 VLGNGKTLVGNSVNSFSLKGKKFPLVYGKGASRECKHLEASLCYSGCLDRTLVKGKIVLC 363

Query: 389 KAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTI 448
              +G + A  AGA GAI L  +  ++SF+LP P ++L      +V +Y  S   P+  I
Sbjct: 364 DDVNGRTEAKRAGALGAI-LPISFEDISFILPLPGLSLTEDKLNAVKSYLNSTKKPSANI 423

Query: 449 FRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRS 508
            +S A KD  AP +A FSSRGPN  + +ILKPD +APGV+ILA+F P+ +P+ +  DKR 
Sbjct: 424 LKSEAIKDNAAPEVASFSSRGPNPIISDILKPDASAPGVDILAAFPPVLSPTDDTADKRH 483

Query: 509 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTA---KQIVRTDGILIREF 568
           V++S++SGTSM+CPH AGVAA+VK+ HP+WS +AIKSAIMTTA       R++G    EF
Sbjct: 484 VKYSVMSGTSMACPHAAGVAAHVKAAHPDWSASAIKSAIMTTAWPMNVTERSEG----EF 543

Query: 569 LYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLAR 628
            +GSG ++P  AI PGLVYE  + D++ + C  GY ++ +   +G++S CSK     L R
Sbjct: 544 AFGSGHVNPVTAIHPGLVYETQKSDYIQLFCGLGYTAEKIRQISGDNSSCSKAARNTLPR 603

Query: 629 DLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKP 688
           DLNYP+M A V  ++ F +KF RTVTNVG ANSTY++KI          SR  L + V P
Sbjct: 604 DLNYPSMAAKVAVEESFTIKFHRTVTNVGNANSTYKAKIF---------SRSSLKIKVVP 663

Query: 689 QELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIVV 732
           + L F+ L EKKSF VT+VG  ++   + S++L+WSD  H VRSPIVV
Sbjct: 664 EALSFKSLKEKKSFAVTIVGRDLTYNSILSASLVWSDGSHSVRSPIVV 696

BLAST of CmaCh02G012700 vs. NCBI nr
Match: gi|743865611|ref|XP_011032225.1| (PREDICTED: cucumisin-like [Populus euphratica])

HSP 1 Score: 719.2 bits (1855), Expect = 7.6e-204
Identity = 376/708 (53.11%), Postives = 489/708 (69.07%), Query Frame = 1

Query: 29  RKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEA 88
           ++ +IVYMG++     + S+HHL+LL+ V+  SS +E   +RSY RSFNGF AKLT  EA
Sbjct: 4   KQEYIVYMGSLPEGEYSPSSHHLSLLQEVVKDSS-SENVLVRSYKRSFNGFSAKLTSEEA 63

Query: 89  EQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELES 148
           ++LA+   VVS+F S   + QTTRSWD++GF    +      +D+I+G IDTGIWPE ES
Sbjct: 64  QKLASKKEVVSIFPSTTLQLQTTRSWDFMGFNVTASGKRGTHSDLIVGVIDTGIWPESES 123

Query: 149 FNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTSMSARDNVGHGSHTASTAAGKLEK 208
           FND+G GPPP +WRG C GG NFTCNNK+IGAR Y+  SARD++GHGSHTASTAAG + K
Sbjct: 124 FNDDGFGPPPRKWRGVCEGGENFTCNNKIIGARHYSFSSARDDLGHGSHTASTAAGNIVK 183

Query: 209 TTGFYGLAGGNARGAVPSSRLAVYKVCNP-DCLEENILAAFDDAIADGVDLITISIGGVG 268
              FYGLA G ARG VPS+R++ YKVC P  C   +IL+AFDDAIADGVD+ITISIG   
Sbjct: 184 KASFYGLAQGTARGGVPSARISAYKVCGPGSCQSSDILSAFDDAIADGVDIITISIGANQ 243

Query: 269 GFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKV 328
              F+ D +AIG +HSMAKGILT+QSAGN GPV G+V SV PW+FTVAA++TDR I+DKV
Sbjct: 244 AQEFDTDVIAIGGFHSMAKGILTLQSAGNNGPVLGSVASVAPWIFTVAASSTDRRIIDKV 303

Query: 329 VLGDGNTVNGYSVNTFA-PNQNVPLIYATNASRNCSSKNAEICSDGCLDPLLVKGKIVQC 388
           +LG+G T+ G SVN+F+   +  PL+Y   ASR C    A +C  GCLD  LVKGKIV C
Sbjct: 304 ILGNGKTLVGNSVNSFSLKGKKFPLVYGKVASRECKHLEASLCYSGCLDRALVKGKIVVC 363

Query: 389 KAFDGASRAFNAGAAGAIVLNDNAANVSFVLPFPAIALKMADYKSVANYAISATNPNVTI 448
              +G + A  AGA GAI L  +  ++SF+LP P ++L      +V +Y  S   P+  I
Sbjct: 364 DDVNGRTEAKRAGALGAI-LPISFEDISFILPLPGLSLTEDKLNAVKSYLNSTKKPSANI 423

Query: 449 FRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRS 508
            +S A KD  AP +A FSSRGPN  + +ILKPD +APG +ILA+F P+ +P+ +  DKR 
Sbjct: 424 LKSEAIKDNAAPEVASFSSRGPNPIISDILKPDASAPGADILAAFPPVLSPTDDTADKRH 483

Query: 509 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTA---KQIVRTDGILIREF 568
           V++S++SGTSM+CPH AGVAAYVK+ HP+WS +AIKSAIMTTA       R+DG    EF
Sbjct: 484 VKYSVMSGTSMACPHAAGVAAYVKAAHPDWSASAIKSAIMTTAWPMNVTERSDG----EF 543

Query: 569 LYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLAR 628
            +GSG I+P  AI PGLVYE  + D++ + C  GY ++ +   +G++S CSK     L R
Sbjct: 544 AFGSGHINPATAIHPGLVYETQKSDYIQLFCGLGYTAEKIRQISGDNSSCSKAARNTLPR 603

Query: 629 DLNYPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKP 688
           DLNYP+M A V  ++ F +KF RTVTNVG ANSTY++KI          SR  L + V P
Sbjct: 604 DLNYPSMAAKVAVEESFRIKFHRTVTNVGRANSTYKAKIF---------SRSSLKIKVVP 663

Query: 689 QELWFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIVV 732
           + L F+ L EKKSF VT+VG  ++   + S++L+WSD  H VR+PIVV
Sbjct: 664 EALSFKSLKEKKSFAVTIVGRDLTHNSILSASLVWSDGSHSVRNPIVV 696

BLAST of CmaCh02G012700 vs. NCBI nr
Match: gi|641831574|gb|KDO50629.1| (hypothetical protein CISIN_1g044745mg, partial [Citrus sinensis])

HSP 1 Score: 715.3 bits (1845), Expect = 1.1e-202
Identity = 388/764 (50.79%), Postives = 513/764 (67.15%), Query Frame = 1

Query: 1   MAPDNFPILFSFIAALLAAILSSANGS-----ERKA-----------------HIVYMGA 60
           MA + F +LF+F++ +L   +S   G+     +RKA                 HIVY+G+
Sbjct: 1   MAKNGF-LLFNFLSFILFLPMSILGGAITSQDDRKASMDICFSALVVLNFLMVHIVYLGS 60

Query: 61  IQNRAMAESTHHLNLLRSVIGTSSVTEGSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVV 120
           +       S+ H ++L+ VIG SSV E   +RSY RSFNGF AKLT  E ++LA+M+GVV
Sbjct: 61  LFRGEYETSSQHQSILQEVIGDSSV-ENVLVRSYKRSFNGFAAKLTDHERQKLASMEGVV 120

Query: 121 SVFESKVFKTQTTRSWDYLGFPAKPTRNLAGETDVIIGSIDTGIWPELESFNDEGIGPPP 180
           SVF S+  +  TTRSWD++G     TR  + E+D+I+G ID+GIWPE ESF+DEG GP P
Sbjct: 121 SVFPSRTLQLHTTRSWDFMGLNQSITRKRSVESDIIVGVIDSGIWPESESFSDEGFGPAP 180

Query: 181 ARWRGTCAGGGNFTCNNKVIGARFYTS-----MSARDNVGHGSHTASTAAGKLEKTTGFY 240
            +W+G C GG NFTCNNK+IGAR+YT+      +ARD  GHG+HTASTA+G   K   F+
Sbjct: 181 KKWKGACKGGRNFTCNNKIIGARYYTTDDISGNTARDIQGHGTHTASTASGNEVKDASFF 240

Query: 241 GLAGGNARGAVPSSRLAVYKVCNPD--CLEENILAAFDDAIADGVDLITISIGGVGGFNF 300
           G+  G ARG VPS+R+A YKVC+P+  C E  IL AFDDAIADGVD+ITIS+GG    NF
Sbjct: 241 GVGQGTARGGVPSARIAAYKVCSPELGCAETAILGAFDDAIADGVDIITISLGGQNTLNF 300

Query: 301 EHDSMAIGSYHSMAKGILTVQSAGNYGPVDGTVGSVVPWVFTVAATTTDRTIVDKVVLGD 360
             D +AIGS+H+MAKG+LT+ SAGN GP  G+  SV PW+ +VAA+ TDR  VDKVVLG 
Sbjct: 301 TQDVIAIGSFHAMAKGVLTLHSAGNSGPFIGSTVSVAPWLMSVAASNTDRLFVDKVVLGS 360

Query: 361 GNTVNGYSVNTF-APNQNVPLIYATNASRNCSSK-NAEICSD--GCLDPLLVKGKIVQCK 420
           G T+ GYS+N+F +  +  PL+   + SR C S  + ++C+D  GC+D  L KGKIV C+
Sbjct: 361 GQTLVGYSINSFSSKGKTFPLVDGMDVSRPCESDFDPQLCTDGQGCIDSRLAKGKIVICQ 420

Query: 421 AFDGASRAFNAGAAGAIVLNDNAAN-VSFVLPFPAIALKMADYKSVANYAISATNPNVTI 480
           +FDG +    AGA G++ LND   N VS V+  PA+AL   ++ S+ +Y  S   P   I
Sbjct: 421 SFDGFNEVHKAGAEGSVSLNDVEFNKVSSVVSLPAVALNEDNFNSIYSYLKSTKKPEANI 480

Query: 481 FRSVATKDPYAPTIADFSSRGPNMWMLEILKPDIAAPGVEILASFSPIAAPSGNVGDKRS 540
             + A KD  AP +ADFSSRGPN  + +ILKPDI+APGV+ILA+FSP+ A S +  DKR 
Sbjct: 481 LSTEAVKDSEAPVVADFSSRGPNEIVPDILKPDISAPGVDILAAFSPLGAVSDDPEDKRQ 540

Query: 541 VEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPAAIKSAIMTTAKQIVRTDGILIREFLYG 600
            +F+++SGTSMSCPH AGVAAYVKSFHP+WSP+AIKSAIMTTA   + +      EF +G
Sbjct: 541 AKFNVVSGTSMSCPHAAGVAAYVKSFHPDWSPSAIKSAIMTTAWP-MNSSKNKDAEFAFG 600

Query: 601 SGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYDSKTMEAFAGNDSVCSKTWTKFLARDLN 660
           SG I+P  A+ PGLVYE FE+D++ +LC+ GYD + +   +GN S C K   K   +DLN
Sbjct: 601 SGHINPVEAVNPGLVYETFEQDYIIMLCSMGYDERNIGKISGNISTCPKGSDKATPKDLN 660

Query: 661 YPAMVAHVLPKKPFVVKFQRTVTNVGVANSTYRSKILSFSGVNVLKSREKLNVSVKPQEL 720
           YP+M A V P K F + F RTVTNVG+ANSTY++KIL  S +        +++ V P+ L
Sbjct: 661 YPSMAAQVSPGKSFTINFPRTVTNVGLANSTYKAKILQNSKI--------VSIKVVPESL 720

Query: 721 WFEHLNEKKSFVVTVVGGAISTEIVFSSALIWSDLDHEVRSPIV 731
            F+ LNEKKSF VTV G  +    + S++L+WSD +H VRSPIV
Sbjct: 721 SFKSLNEKKSFSVTVTGKGLPNGAIVSTSLMWSDGNHRVRSPIV 753

BLAST of CmaCh02G012700 vs. NCBI nr
Match: gi|470113636|ref|XP_004293027.1| (PREDICTED: cucumisin-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 715.3 bits (1845), Expect = 1.1e-202
Identity = 393/734 (53.54%), Postives = 501/734 (68.26%), Query Frame = 1

Query: 6   FPILFSFIAALLAAILSSANGSERKAHIVYMGAIQNRAMAESTHHLNLLRSVIGTSSVTE 65
           FPIL      L  ++L  AN  +RK HIVY+G++ N   + S+HHL++L+ V+  S   +
Sbjct: 13  FPILI-----LSLSLLCKANDEDRKVHIVYLGSLPNTVYSPSSHHLDMLQKVVQGSGSLK 72

Query: 66  GSYIRSYGRSFNGFVAKLTGAEAEQLAAMDGVVSVFESKVFKTQTTRSWDYLGFPAKPTR 125
              IRSY RSFNGF AKLT  E E+LA M  VVS+F+S  FK QTTRSWD++GF  K  R
Sbjct: 73  NYLIRSYKRSFNGFAAKLTDQEREKLAYMKEVVSIFQSTKFKLQTTRSWDFMGFNEKINR 132

Query: 126 NLAGETDVIIGSIDTGIWPELESFNDEGIGPPPARWRGTCAGGGNFTCNNKVIGARFYTS 185
           N+  E+DVIIG ID+GIWPE ESF D+G GP P +W+G C GG NFTCNNK+IGAR+Y+S
Sbjct: 133 NVTTESDVIIGVIDSGIWPESESFQDKGFGPVPKKWKGACNGGKNFTCNNKLIGARYYSS 192

Query: 186 M-SARDNVGHGSHTASTAAGKLEKTTGFYGLAGGNARGAVPSSRLAVYKVCNPD--CLEE 245
           + SARD VGHGSHTAS AAG   K   FYGLA G ARG VPS+R+A Y+VC+ D  C   
Sbjct: 193 IQSARDEVGHGSHTASVAAGNSVKDVSFYGLAQGTARGGVPSARIAAYQVCDLDSFCSGH 252

Query: 246 NILAAFDDAIADGVDLITISIGGVGGFNFEHDSMAIGSYHSMAKGILTVQSAGNYGPVDG 305
           ++L AFDDAIADGVD+ITISIG     +F  D +AIGS+H+MAKGILT  SAGN GP   
Sbjct: 253 DVLTAFDDAIADGVDIITISIGTDLPSDFYKDPIAIGSFHAMAKGILTSNSAGNEGPAGD 312

Query: 306 TVGSVVPWVFTVAATTTDRTIVDKVVLGDGNTVNGYSVNTFAPN-QNVPLIYATNASRN- 365
           TV S  PW  TVAA+  DR I+DKVVLG+G+ + G SVNTF  N  N PLIY  +ASR+ 
Sbjct: 313 TVSSAAPWTLTVAASRIDRRIIDKVVLGNGSIIVGTSVNTFTLNGTNFPLIYGQDASRHE 372

Query: 366 CSSKNAEICSDGCLDPLLVKGKIVQCKAFDGAS-RAFNAGAAGAIVLNDNAANVSFVLPF 425
           CS   A  C + CLD   VKGKIV C AFD +   A  AGA G+I+ ND   + S VL  
Sbjct: 373 CSDSQAGYCDEDCLDSDAVKGKIVLCDAFDESDVVALKAGALGSILNNDGTGDFSNVLSL 432

Query: 426 PAIALKMADYKSVANYAISATNPNVTIFRSVATKDPYAPTIADFSSRGPNMWMLEILKPD 485
           PAIAL   +Y +V  Y  S  +P   I RS    D  AP +A FSSRGPN    +I+KPD
Sbjct: 433 PAIALSHENYIAVKFYMNSTRDPRANILRSEVINDAAAPIVASFSSRGPNPITPDIIKPD 492

Query: 486 IAAPGVEILASFSPIAAPSGNVGDKRSVEFSILSGTSMSCPHVAGVAAYVKSFHPNWSPA 545
           ++APGV+ILA++SP+A+ S +  DKR V++ ILSGTSMSCPH A VAAYVKSFHP+WSP+
Sbjct: 493 LSAPGVDILAAYSPVASISVSPEDKRHVKYHILSGTSMSCPHAAAVAAYVKSFHPDWSPS 552

Query: 546 AIKSAIMTTAKQIVRTDGILIREFLYGSGLIDPNRAIEPGLVYEIFEKDHLNVLCAKGYD 605
           AIKS++MTTA  +  T+     EF YGSG I+P +A++PGLVYE  ++D + +LC + YD
Sbjct: 553 AIKSSLMTTAWPMNNTNN-APAEFAYGSGHINPVKAVDPGLVYETSKEDFIKLLCME-YD 612

Query: 606 SKTMEAFAGNDSVCSKTWTKFLARDLNYPAM-VAHVLPKKPFVVKFQRTVTNVGVANSTY 665
              +   +G++S C     K   +DLNYP+M  A++ PKKPF + F RTV NVG+ANSTY
Sbjct: 613 EAKVRLISGDNSSCPTGSDKGSPKDLNYPSMAAANITPKKPFTINFHRTVKNVGLANSTY 672

Query: 666 RSKILSFSGVNVLKSREKLNVSVKPQELWFEHLNEKKSFVVTVVG-GAISTEIVFSSALI 725
                     +++++ +++++ V P  L F+ LNE+KSF VTVVG G I   ++ SS+LI
Sbjct: 673 --------SASIVQNSKEVDIKVTPDILSFKSLNEEKSFDVTVVGKGLIGGSLIISSSLI 731

Query: 726 WSDLDHEVRSPIVV 732
           WSD +H VRSPIVV
Sbjct: 733 WSDNNHSVRSPIVV 731

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SBT43_ARATH4.0e-19752.27Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1[more]
SBT4C_ARATH7.4e-19150.20Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana GN=SBT4.12 PE=2 SV=1[more]
SBT44_ARATH5.9e-18849.46Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1[more]
SBT45_ARATH1.9e-18650.27Subtilisin-like protease SBT4.5 OS=Arabidopsis thaliana GN=SBT4.5 PE=3 SV=1[more]
SBT4D_ARATH1.9e-18649.52Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
B9HPB8_POPTR3.7e-20553.11Subtilase family protein OS=Populus trichocarpa GN=POPTR_0009s04290g PE=4 SV=1[more]
A0A067E600_CITSI7.7e-20350.79Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g044745mg PE=4 S... [more]
A0A061GWL7_THECC8.5e-20253.00Subtilase family protein, putative isoform 1 OS=Theobroma cacao GN=TCM_041957 PE... [more]
A0A061GXN7_THECC1.4e-20153.00Subtilase 4.13, putative isoform 2 OS=Theobroma cacao GN=TCM_041957 PE=4 SV=1[more]
W9RUC3_9ROSA3.2e-20151.84Uncharacterized protein OS=Morus notabilis GN=L484_025323 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G59190.11.6e-19652.00 subtilase family protein[more]
AT5G59090.14.2e-19250.20 subtilase 4.12[more]
AT5G59100.13.3e-18949.46 Subtilisin-like serine endopeptidase family protein[more]
AT3G46840.11.1e-18750.27 Subtilase family protein[more]
AT5G59120.11.1e-18749.52 subtilase 4.13[more]
Match NameE-valueIdentityDescription
gi|568877765|ref|XP_006491889.1|2.8e-20651.87PREDICTED: subtilisin-like protease SBT4.8 [Citrus sinensis][more]
gi|224106379|ref|XP_002314147.1|5.3e-20553.11subtilase family protein [Populus trichocarpa][more]
gi|743865611|ref|XP_011032225.1|7.6e-20453.11PREDICTED: cucumisin-like [Populus euphratica][more]
gi|641831574|gb|KDO50629.1|1.1e-20250.79hypothetical protein CISIN_1g044745mg, partial [Citrus sinensis][more]
gi|470113636|ref|XP_004293027.1|1.1e-20253.54PREDICTED: cucumisin-like [Fragaria vesca subsp. vesca][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR003137PA_domain
IPR010259S8pro/Inhibitor_I9
IPR015500Peptidase_S8_subtilisin-rel
IPR023828Peptidase_S8_Ser-AS
Vocabulary: Molecular Function
TermDefinition
GO:0004252serine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004252 serine-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G012700.1CmaCh02G012700.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainGENE3DG3DSA:3.40.50.200coord: 456..576
score: 1.9E-69coord: 111..329
score: 1.9
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 131..568
score: 1.8
IPR000209Peptidase S8/S53 domainunknownSSF52743Subtilisin-likecoord: 109..358
score: 1.83E-66coord: 460..580
score: 1.83
IPR003137PA domainPFAMPF02225PAcoord: 370..428
score: 1.
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 32..108
score: 2.2
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 130..149
score: 1.1E-10coord: 513..529
score: 1.1E-10coord: 190..203
score: 1.1
IPR015500Peptidase S8, subtilisin-relatedPANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 9..731
score:
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 514..524
scor
NoneNo IPR availablePANTHERPTHR10795:SF399CUCUMISIN-LIKE-RELATEDcoord: 9..731
score:

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
CmaCh02G012700CmoCh02G013100Cucurbita moschata (Rifu)cmacmoB619
CmaCh02G012700Lsi10G010830Bottle gourd (USVL1VR-Ls)cmalsiB540
CmaCh02G012700Cp4.1LG05g04680Cucurbita pepo (Zucchini)cmacpeB646
The following gene(s) are paralogous to this gene:

None