CmaCh02G006910 (gene) Cucurbita maxima (Rimu)

NameCmaCh02G006910
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionEukaryotic aspartyl protease family protein, putative
LocationCma_Chr02 : 4226060 .. 4227864 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGCCATTCTCATCTTCCTCCTTGCTTCATCTGTTTTCTCTGACAGGAACGGAGCAATGTCGTCGATTTCTCATCTTTTAATCCTTTTCTTCGTCGTCTTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAATGCCAATAATCTGAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTGGATCTGATACACCGCCACCATCCGGAAGTGGTTAAGAGGCTTCATGATGAAATTAAGGTGGATAAGATGGAGGATCGCATCAAGGATATTCGCTATCACGATCAATCTCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGTGAAGGAGGCGTCGGGTTCGAATCATCCTCCACATTCGCAGACGCCAATAGCATTGAAAACATACCCCGGCGCTGATTTCGGTAGCAGTGAGTTTTTCGTGCAATTGAAAGTGGGAACGCCGCCGCAGAAGTTCACGATGATTGCAGATACCGGAAGTGACCTATTGTGGACGAGATGCAGATACCGGCGGTGCAGGGGAGATTGCAGCAACCCCTCTCCGATCCATAAAATGCGTAACAAAATGAGAGAGAGATTCAATTACGCGCTTTATGCGAATCAGTCATCTTCTTTCTCCCCAATTCCTTGTTCCTCCAAGCAGTGTATCCAGGATTTCTCTGAGCTCGGCGGCCAACCCGATTGTCCAACCCCCAACACCCCTTGTTCCTATACCTACAGGTATTAATAATTAATATTATTATTATTATTATTATTTTAAATAAAATTTTGGTGGGGACCATTATAACAATAAATGGATGTTGGTTGGTCTGTTTAAAAAATAAATAAATAACAGCTACTTAAGTGGGGACCGCGCGATGGGAATATTCGCAACCGAGACGGTAACAGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATACGGCTGCACCGAAGAAATGACTGACAGCCAGTTCTTGGACGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACGTCGGCGGCGGCTTCTCCTACTGCCTCGCCGACCACCTCCGCAATATAACCGCCATTAGCTACTTCGTCTTCGGCACCCCCTCCCCCAAGACCTTCTCCGCCTCCACATCCTCTCCCATCGGCCCCCCCGCCACCACTAAACTCTTCACCGGCGGCCGATACAGCTGCTACTACGGCGTCCAACTGTCCGGAATCTCCGTCGACGGACAGATCCTGAACATCCCCCCTCACGTTTGGAACATCAAGTCCGGTTGCGGCACCATCTTGGACACGGGCACCAGCCTGACGATGCTGACGGCGCCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCCAAGATCGAAAAATTCGGAAGAATGGAAAAGGATGTAAAGGGTGAAAGGGAGAAGAACTTCAAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTTGCCGAAGCTTGGATTCCATTTCGAAGACGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCGATAACTTCTCTGCCCTTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAGACTTTCATTTGGAAATATGATTTACTCAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAGACCTTCTCCATTTTCTTTCATTTATTACTTCCTTCTTATTAATTATACTCATATAAT

mRNA sequence

CGCCATTCTCATCTTCCTCCTTGCTTCATCTGTTTTCTCTGACAGGAACGGAGCAATGTCGTCGATTTCTCATCTTTTAATCCTTTTCTTCGTCGTCTTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAATGCCAATAATCTGAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTGGATCTGATACACCGCCACCATCCGGAAGTGGTTAAGAGGCTTCATGATGAAATTAAGGTGGATAAGATGGAGGATCGCATCAAGGATATTCGCTATCACGATCAATCTCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGTGAAGGAGGCGTCGGGTTCGAATCATCCTCCACATTCGCAGACGCCAATAGCATTGAAAACATACCCCGGCGCTGATTTCGGTAGCAGTGAGTTTTTCGTGCAATTGAAAGTGGGAACGCCGCCGCAGAAGTTCACGATGATTGCAGATACCGGAAGTGACCTATTGTGGACGAGATGCAGATACCGGCGGTGCAGGGGAGATTGCAGCAACCCCTCTCCGATCCATAAAATGCGTAACAAAATGAGAGAGAGATTCAATTACGCGCTTTATGCGAATCAGTCATCTTCTTTCTCCCCAATTCCTTGTTCCTCCAAGCAGTGTATCCAGGATTTCTCTGAGCTCGGCGGCCAACCCGATTGTCCAACCCCCAACACCCCTTGTTCCTATACCTACAGCTACTTAAGTGGGGACCGCGCGATGGGAATATTCGCAACCGAGACGGTAACAGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATACGGCTGCACCGAAGAAATGACTGACAGCCAGTTCTTGGACGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACGTCGGCGGCGGCTTCTCCTACTGCCTCGCCGACCACCTCCGCAATATAACCGCCATTAGCTACTTCGTCTTCGGCACCCCCTCCCCCAAGACCTTCTCCGCCTCCACATCCTCTCCCATCGGCCCCCCCGCCACCACTAAACTCTTCACCGGCGGCCGATACAGCTGCTACTACGGCGTCCAACTGTCCGGAATCTCCGTCGACGGACAGATCCTGAACATCCCCCCTCACGTTTGGAACATCAAGTCCGGTTGCGGCACCATCTTGGACACGGGCACCAGCCTGACGATGCTGACGGCGCCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCCAAGATCGAAAAATTCGGAAGAATGGAAAAGGATGTAAAGGGTGAAAGGGAGAAGAACTTCAAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTTGCCGAAGCTTGGATTCCATTTCGAAGACGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCGATAACTTCTCTGCCCTTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAGACTTTCATTTGGAAATATGATTTACTCAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAGACCTTCTCCATTTTCTTTCATTTATTACTTCCTTCTTATTAATTATACTCATATAAT

Coding sequence (CDS)

ATGTCGTCGATTTCTCATCTTTTAATCCTTTTCTTCGTCGTCTTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAATGCCAATAATCTGAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTGGATCTGATACACCGCCACCATCCGGAAGTGGTTAAGAGGCTTCATGATGAAATTAAGGTGGATAAGATGGAGGATCGCATCAAGGATATTCGCTATCACGATCAATCTCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGTGAAGGAGGCGTCGGGTTCGAATCATCCTCCACATTCGCAGACGCCAATAGCATTGAAAACATACCCCGGCGCTGATTTCGGTAGCAGTGAGTTTTTCGTGCAATTGAAAGTGGGAACGCCGCCGCAGAAGTTCACGATGATTGCAGATACCGGAAGTGACCTATTGTGGACGAGATGCAGATACCGGCGGTGCAGGGGAGATTGCAGCAACCCCTCTCCGATCCATAAAATGCGTAACAAAATGAGAGAGAGATTCAATTACGCGCTTTATGCGAATCAGTCATCTTCTTTCTCCCCAATTCCTTGTTCCTCCAAGCAGTGTATCCAGGATTTCTCTGAGCTCGGCGGCCAACCCGATTGTCCAACCCCCAACACCCCTTGTTCCTATACCTACAGCTACTTAAGTGGGGACCGCGCGATGGGAATATTCGCAACCGAGACGGTAACAGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATACGGCTGCACCGAAGAAATGACTGACAGCCAGTTCTTGGACGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACGTCGGCGGCGGCTTCTCCTACTGCCTCGCCGACCACCTCCGCAATATAACCGCCATTAGCTACTTCGTCTTCGGCACCCCCTCCCCCAAGACCTTCTCCGCCTCCACATCCTCTCCCATCGGCCCCCCCGCCACCACTAAACTCTTCACCGGCGGCCGATACAGCTGCTACTACGGCGTCCAACTGTCCGGAATCTCCGTCGACGGACAGATCCTGAACATCCCCCCTCACGTTTGGAACATCAAGTCCGGTTGCGGCACCATCTTGGACACGGGCACCAGCCTGACGATGCTGACGGCGCCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCCAAGATCGAAAAATTCGGAAGAATGGAAAAGGATGTAAAGGGTGAAAGGGAGAAGAACTTCAAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTTGCCGAAGCTTGGATTCCATTTCGAAGACGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCGATAACTTCTCTGCCCTTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAGACTTTCATTTGGAAATATGATTTACTCAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAG

Protein sequence

MSSISHLLILFFVVFFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA
BLAST of CmaCh02G006910 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.3e-37
Identity = 126/397 (31.74%), Postives = 181/397 (45.59%), Query Frame = 1

Query: 134 GSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFN 193
           G  E+ + + +GTP   F+ I DTGSDL+WT+C    C    S P+PI          FN
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCE--PCTQCFSQPTPI----------FN 151

Query: 194 YALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATE 253
                  SSSFS +PC S+ C QD       P     N  C YTY Y  G    G  ATE
Sbjct: 152 ----PQDSSSFSTLPCESQYC-QDL------PSETCNNNECQYTYGYGDGSTTQGYMATE 211

Query: 254 TVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGF 313
           T T   ++     + +I +GC E+       +GA GLIG+G    S   +       G F
Sbjct: 212 TFTFETSS-----VPNIAFGCGEDNQGFGQGNGA-GLIGMGWGPLSLPSQLGV----GQF 271

Query: 314 SYCLADHLRNITAISYFVFGTPSPKTF---SASTSSPIGPPATTKLFTGGRYSCYYGVQL 373
           SYC+              +G+ SP T    SA++  P G P+TT L        YY + L
Sbjct: 272 SYCMTS------------YGSSSPSTLALGSAASGVPEGSPSTT-LIHSSLNPTYYYITL 331

Query: 374 SGISVDGQILNIPPHVWNIKSGC--GTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRM 433
            GI+V G  L IP   + ++     G I+D+GT+LT L   A++AV +A   +I      
Sbjct: 332 QGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINL---- 391

Query: 434 EKDVKGEREKNFKLCFND-TEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAI 493
                 E       CF   ++ +   +P++   F DG V    +++ ++S +    C+A+
Sbjct: 392 --PTVDESSSGLSTCFQQPSDGSTVQVPEISMQF-DGGVLNLGEQNILISPAEGVICLAM 435

Query: 494 TSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
            S     I+I GNI QQ     YDL   +V+F P+ C
Sbjct: 452 GSSSQLGISIFGNIQQQETQVLYDLQNLAVSFVPTQC 435

BLAST of CmaCh02G006910 vs. Swiss-Prot
Match: CDR1_ARATH (Aspartic proteinase CDR1 OS=Arabidopsis thaliana GN=CDR1 PE=1 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.8e-34
Identity = 113/391 (28.90%), Postives = 177/391 (45.27%), Query Frame = 1

Query: 135 SSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFNY 194
           S E+ + + +GTPP     IADTGSDLLWT+C            +P      ++   F+ 
Sbjct: 87  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQC------------APCDDCYTQVDPLFD- 146

Query: 195 ALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATET 254
                 SS++  + CSS QC    + L  Q  C T +  CSY+ SY       G  A +T
Sbjct: 147 ---PKTSSTYKDVSCSSSQC----TALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 206

Query: 255 VTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFS 314
           +T+  ++ +  QLK+I+ GC      + F     G++GLG    S + K   +++ G FS
Sbjct: 207 LTLGSSDTRPMQLKNIIIGCGHNNAGT-FNKKGSGIVGLGGGPVSLI-KQLGDSIDGKFS 266

Query: 315 YCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSCYYGVQLSGIS 374
           YCL          S   FGT +  + S   S+P+   A+ + F        Y + L  IS
Sbjct: 267 YCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETF--------YYLTLKSIS 326

Query: 375 VDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKG 434
           V  + +           G   I+D+GT+LT+L    +  + +A+A  I      + + K 
Sbjct: 327 VGSKQIQYSGSDSESSEG-NIIIDSGTTLTLLPTEFYSELEDAVASSI------DAEKKQ 386

Query: 435 EREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAITSLPFPS 494
           + +    LC++ T      +P +  HF DGA  +    +  V  S    C A      PS
Sbjct: 387 DPQSGLSLCYSAT--GDLKVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRG--SPS 435

Query: 495 INILGNIIQQTFIWKYDLLKGSVTFAPSDCA 526
            +I GN+ Q  F+  YD +  +V+F P+DCA
Sbjct: 447 FSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of CmaCh02G006910 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 1.7e-32
Identity = 120/398 (30.15%), Postives = 173/398 (43.47%), Query Frame = 1

Query: 134 GSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFN 193
           G  E+ + L +GTP Q F+ I DTGSDL+WT+C+            P  +  N+    FN
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQ------------PCTQCFNQSTPIFN 150

Query: 194 YALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATE 253
                  SSSFS +PCSS+ C     +    P C   N  C YTY Y  G    G   TE
Sbjct: 151 ----PQGSSSFSTLPCSSQLC-----QALSSPTC--SNNFCQYTYGYGDGSETQGSMGTE 210

Query: 254 TVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGF 313
           T+T    +     + +I +GC E        +GA GL+G+G    S   +         F
Sbjct: 211 TLTFGSVS-----IPNITFGCGENNQGFGQGNGA-GLVGMGRGPLSLPSQLDVTK----F 270

Query: 314 SYCLADHLRNITAISYFVFGTPSPKTF---SASTSSPIGPPATTKLFTGGRYSCYYGVQL 373
           SYC+               G+ +P      S + S   G P TT L    +   +Y + L
Sbjct: 271 SYCMTP------------IGSSTPSNLLLGSLANSVTAGSPNTT-LIQSSQIPTFYYITL 330

Query: 374 SGISVDGQILNIPPHVWNIKSGCGT---ILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGR 433
           +G+SV    L I P  + + S  GT   I+D+GT+LT     A+ +V      + E   +
Sbjct: 331 NGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSV------RQEFISQ 390

Query: 434 MEKDVKGEREKNFKLCF-NDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIA 493
           +   V       F LCF   ++ +   +P    HF DG   E P  +Y +S S    C+A
Sbjct: 391 INLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHF-DGGDLELPSENYFISPSNGLICLA 434

Query: 494 ITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           + S     ++I GNI QQ  +  YD     V+FA + C
Sbjct: 451 MGS-SSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of CmaCh02G006910 vs. Swiss-Prot
Match: APF2_ARATH (Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.9e-31
Identity = 121/400 (30.25%), Postives = 175/400 (43.75%), Query Frame = 1

Query: 130 GADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMR 189
           G   GS E+F +L VGTP +   M+ DTGSD++W +C    CR   S   PI   R    
Sbjct: 134 GLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQC--APCRRCYSQSDPIFDPR---- 193

Query: 190 ERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGI 249
                     +S +++ IPCSS  C +  S       C T    C Y  SY  G   +G 
Sbjct: 194 ----------KSKTYATIPCSSPHCRRLDS-----AGCNTRRKTCLYQVSYGDGSFTVGD 253

Query: 250 FATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNV 309
           F+TET+T R       ++K +  GC  +  +     GA GL+GLG    SF  +   +  
Sbjct: 254 FSTETLTFR-----RNRVKGVALGCGHD--NEGLFVGAAGLLGLGKGKLSFPGQTG-HRF 313

Query: 310 GGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSCYYGVQ 369
              FSYCL D   +           PS   F  +  S I     T L +  +   +Y V 
Sbjct: 314 NQKFSYCLVDRSAS---------SKPSSVVFGNAAVSRIA--RFTPLLSNPKLDTFYYVG 373

Query: 370 LSGISVDG-QILNIPPHVWNIK--SGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFG 429
           L GISV G ++  +   ++ +      G I+D+GTS+T L  PA+ A+ +A       F 
Sbjct: 374 LLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDA-------FR 433

Query: 430 RMEKDVKGEREKN-FKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCI 489
              K +K   + + F  CF+ +  N   +P +  HF  GA    P  +Y++         
Sbjct: 434 VGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFC 485

Query: 490 AITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA 526
              +     ++I+GNI QQ F   YDL    V FAP  CA
Sbjct: 494 FAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

BLAST of CmaCh02G006910 vs. Swiss-Prot
Match: ASPG1_ARATH (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 1.5e-28
Identity = 115/449 (25.61%), Postives = 189/449 (42.09%), Query Frame = 1

Query: 83  DQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQL 142
           D SR+  I A + +   VE  +    +   +    +    +      GA  GS E+F ++
Sbjct: 109 DSSRVAGIVAKIRFA--VEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRI 168

Query: 143 KVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSS 202
            VGTP ++  ++ DTGSD+ W +C    C        P+          FN       SS
Sbjct: 169 GVGTPAKEMYLVLDTGSDVNWIQC--EPCADCYQQSDPV----------FN----PTSSS 228

Query: 203 SFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNG 262
           ++  + CS+ QC      L     C   +  C Y  SY  G   +G  AT+TVT     G
Sbjct: 229 TYKSLTCSAPQC-----SLLETSAC--RSNKCLYQVSYGDGSFTVGELATDTVTF----G 288

Query: 263 KEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLR 322
              ++ ++  GC  +  +     GA GL+GLG  + S   +    +    FSYCL D   
Sbjct: 289 NSGKINNVALGCGHD--NEGLFTGAAGLLGLGGGVLSITNQMKATS----FSYCLVDR-- 348

Query: 323 NITAISYFVFGTPSPKTFSASTSSPI--GPPATTKLFTGGRYSCYYGVQLSGISVDGQIL 382
                        S K+ S   +S    G  AT  L    +   +Y V LSG SV G+ +
Sbjct: 349 ------------DSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKV 408

Query: 383 NIPPHVWNI--KSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREK 442
            +P  ++++      G ILD GT++T L   A++++ +A         +    +      
Sbjct: 409 VLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSI-----S 468

Query: 443 NFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIV---SASYQCSCIAITSLPFPSI 502
            F  C++ +  +   +P + FHF  G   + P ++Y++    +   C   A TS    S+
Sbjct: 469 LFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTS---SSL 500

Query: 503 NILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           +I+GN+ QQ     YDL K  +  + + C
Sbjct: 529 SIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of CmaCh02G006910 vs. TrEMBL
Match: A0A0A0KG92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.7e-140
Identity = 269/539 (49.91%), Postives = 355/539 (65.86%), Query Frame = 1

Query: 1   MSSISHL--LILFFVVFFF----SPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHR 60
           MS IS+      FF++FFF    S    A+ D+ N  N     + + +EQE ++ DL+HR
Sbjct: 8   MSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEIIKFDLLHR 67

Query: 61  HHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVE-----NAEEKVKE 120
           HHP+V +++H ++K+  + +R+KDI  HD +R R+IS  +N  +V +      AE   +E
Sbjct: 68  HHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAEAEAATEE 127

Query: 121 --ASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCR 180
             A  +  PP + TPI ++   GADFGSSE+FV+LKVGTP Q F +IADTGSDL W +CR
Sbjct: 128 EVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSDLTWMKCR 187

Query: 181 YRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDC 240
           YRRC G+CS+ +  HK +N+ ++RF +A  AN SSSF  + CSS  C  D ++L    +C
Sbjct: 188 YRRCFGNCSS-NVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLADLFAVREC 247

Query: 241 PTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGA 300
             P +PC Y YSY  G  A GIFA ET+TV LTNGKEKQL + + GCTE +  S F  GA
Sbjct: 248 HNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQGSVF-GGA 307

Query: 301 DGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSP 360
           DG++GLG+S YS  YKAAEN  GGGFSYCL DHL +  AISYFV G P+P T SASTSS 
Sbjct: 308 DGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPST-SASTSSA 367

Query: 361 IGPP--ATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTM 420
             P     TKL+ G  YS +YGV L GIS +G +LNIP  VW+I SG GTI+D+GTSLT+
Sbjct: 368 KLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIIDSGTSLTI 427

Query: 421 LTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGA 480
           L APA D V+EA+ P+++KF ++E +        F  CFN++++   M PKL FHF DG 
Sbjct: 428 LAAPAFDMVMEALTPRLKKFQQLEIE-------PFDFCFNNSQYTHEMAPKLRFHFGDGT 487

Query: 481 VFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           VFEPP +SYIVS     SCI   S+PFP+ NI+GNI+QQ  +W++D  K  V FAPS+C
Sbjct: 488 VFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSEC 536

BLAST of CmaCh02G006910 vs. TrEMBL
Match: A5BLS9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015630 PE=3 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 9.6e-91
Identity = 190/478 (39.75%), Postives = 269/478 (56.28%), Query Frame = 1

Query: 47  VRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEK 106
           +RL+LIHRH P+V+ R   +++      R+K++ + D  R   I   L   ++      K
Sbjct: 1   MRLELIHRHSPQVMGRPKTQLQ------RLKELVHSDSVRQLMILHKLRGGQI---PRRK 60

Query: 107 VKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRC 166
            KE   S+    S   I +  +P AD+G  ++FV  KVGTP QKF ++ADTGSDL W  C
Sbjct: 61  AKEVLSSSSGRGSDDAIEVPMHPAADYGIGQYFVAFKVGTPSQKFMLVADTGSDLTWMSC 120

Query: 167 RYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPD 226
           +Y     +CSN       R   R R     +AN SSSF  IPC +  C  +  +L    +
Sbjct: 121 KYHCRSRNCSN-------RKARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTN 180

Query: 227 CPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDG 286
           CPTP TPC Y Y Y  G  A+G FA ETVTV L  G++ +L ++L GC+E      F   
Sbjct: 181 CPTPLTPCGYDYRYSDGSTALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSF-QA 240

Query: 287 ADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSS 346
           ADG++GLG S YSF  KAAE   GG FSYCL DHL +    +Y  FG+      S S  +
Sbjct: 241 ADGVMGLGYSKYSFAIKAAE-KFGGKFSYCLVDHLSHKNVSNYLTFGS------SRSKEA 300

Query: 347 PIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTML 406
            +     T+L   G  + +Y V + GIS+ G +L IP  VW++K   GTILD+G+SLT L
Sbjct: 301 LLNNMTYTELVL-GMVNSFYAVNMMGISIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFL 360

Query: 407 TAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAV 466
           T PA+  V+ A+   + KF ++E D+        + CFN T +   ++P+L FHF DGA 
Sbjct: 361 TEPAYQPVMAALRVSLLKFRKVEMDI-----GPLEYCFNSTGFEESLVPRLVFHFADGAE 420

Query: 467 FEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           FEPP +SY++SA+    C+   S+ +P  +++GNI+QQ  +W++DL    + FAPS C
Sbjct: 421 FEPPVKSYVISAADGVRCLGFVSVAWPGTSVVGNIMQQNHLWEFDLGLKKLGFAPSSC 448

BLAST of CmaCh02G006910 vs. TrEMBL
Match: F6H9S0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0085g01110 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 8.1e-90
Identity = 189/478 (39.54%), Postives = 268/478 (56.07%), Query Frame = 1

Query: 47  VRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEK 106
           +RL+LIHRH P+V+ R   +++      R+K++ + D  R   I   L   ++      K
Sbjct: 41  MRLELIHRHSPQVMGRPKTQLQ------RLKELVHSDSVRQLMILHKLRGGQI---PRRK 100

Query: 107 VKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRC 166
            KE   S+    S   I +  +P AD+G  ++ V  KVGTP QKF ++ADTGSDL W  C
Sbjct: 101 AKEVLSSSSGRGSDDAIEVPMHPAADYGIGQYSVAFKVGTPSQKFMLVADTGSDLTWMSC 160

Query: 167 RYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPD 226
           +Y     +CSN       R   R R     +AN SSSF  IPC +  C  +  +L    +
Sbjct: 161 KYHCRSRNCSN-------RKARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTN 220

Query: 227 CPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDG 286
           CPTP TPC Y Y Y  G  A+G FA ETVTV L  G++ +L ++L GC+E      F   
Sbjct: 221 CPTPLTPCGYDYRYSDGSTALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSF-QA 280

Query: 287 ADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSS 346
           ADG++GLG S YSF  KAAE   GG FSYCL DHL +    +Y  FG+      S S  +
Sbjct: 281 ADGVMGLGYSKYSFAIKAAE-KFGGKFSYCLVDHLSHKNVSNYLTFGS------SRSKEA 340

Query: 347 PIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTML 406
            +     T+L   G  + +Y V + GIS+ G +L IP  VW++K   GTILD+G+SLT L
Sbjct: 341 LLNNMTYTELVL-GMVNSFYAVNMMGISIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFL 400

Query: 407 TAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAV 466
           T PA+  V+ A+   + KF ++E D+        + CFN T +   ++P+L FHF DGA 
Sbjct: 401 TEPAYQPVMAALRVSLLKFRKVEMDI-----GPLEYCFNSTGFEESLVPRLVFHFADGAE 460

Query: 467 FEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           FEPP +SY++SA+    C+   S+ +P  +++GNI+QQ  +W++DL    + FAPS C
Sbjct: 461 FEPPVKSYVISAADGVRCLGFVSVAWPGTSVVGNIMQQNHLWEFDLGLKKLGFAPSSC 488

BLAST of CmaCh02G006910 vs. TrEMBL
Match: A0A0B0NTS3_GOSAR (Asparticase nepenthesin-1 OS=Gossypium arboreum GN=F383_00615 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.6e-85
Identity = 193/520 (37.12%), Postives = 279/520 (53.65%), Query Frame = 1

Query: 7   LLILFFVVFFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRLHDE 66
           +L+ F V+F        V  Q + + ++ + D+N+     + L+LIHRH P+        
Sbjct: 7   ILVPFMVLFSM------VVAQQHVDQMQHQHDSNS-----ITLELIHRHAPQFTNN---- 66

Query: 67  IKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQTPIALK 126
                   R+ D+ YHD  R   I +H    K  +     +K             P+A  
Sbjct: 67  -NPITQHQRLVDLLYHDIIR-HGIMSHRRRAKEEDPLTASIK------------MPLA-- 126

Query: 127 TYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGD--CSNPSPIHKM 186
              G DFG  ++    KVGTP QKF +I DTGSDL W RCRYR  RGD  C++   I++ 
Sbjct: 127 --SGRDFGIGQYITSFKVGTPSQKFWLIVDTGSDLTWIRCRYRCSRGDRSCTSKGRINRK 186

Query: 187 RNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGD 246
           R           +A  SSSF+P+PC S+ C  +   L     CPTP TPC+Y Y Y  G 
Sbjct: 187 R---------VFHAPLSSSFNPVPCFSEMCKVELMNLFSLTTCPTPITPCAYDYRYSDGS 246

Query: 247 RAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKA 306
            AMG+FA ETV+  LTNG++ +L ++L GCT+       L   DG++GL ++ YSF   A
Sbjct: 247 AAMGVFANETVSAGLTNGRKTRLHNVLIGCTDSF-QGPTLQNVDGIMGLANTKYSFATNA 306

Query: 307 AENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSC 366
           A    GG FSYCL DHL ++ A +Y +FGT      + +     G    TKL      S 
Sbjct: 307 AA-TFGGKFSYCLVDHLSHLNATNYIIFGT------NRNQVKVSGNTRHTKLELDAIPS- 366

Query: 367 YYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEK 426
           +Y V + GISV  ++L IP  VW+   G GTI+D+GTSLT L  PA+ AV+EA+   + K
Sbjct: 367 FYAVNVIGISVGNKMLEIPMQVWDASEGGGTIIDSGTSLTFLADPAYQAVMEALKVSVSK 426

Query: 427 FGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSC 486
           + R++ D         + CFN T +N  ++PKL  HF+DGA FEP   SY+++A+ +  C
Sbjct: 427 YQRVKLD-----GVPMEYCFNSTGFNGSLVPKLIIHFDDGARFEPHWNSYVIAAAAEVRC 470

Query: 487 IAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           +      FP+++++GNI+QQ ++W++DL    + FAPS C
Sbjct: 487 LGFLPARFPALSVIGNIMQQNYLWEFDLKGKRLVFAPSSC 470

BLAST of CmaCh02G006910 vs. TrEMBL
Match: W9QQY3_9ROSA (Aspartic proteinase nepenthesin-1 OS=Morus notabilis GN=L484_019203 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.7e-85
Identity = 190/479 (39.67%), Postives = 266/479 (55.53%), Query Frame = 1

Query: 48  RLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKV 107
           RL+L+HR+ P++ ++   +I    ME   K I +H +  LR         ++V +    +
Sbjct: 25  RLELLHRNSPKLSEKW--QIPETTME---KLIEFHRRDVLRH--------RMVSHRRMGI 84

Query: 108 KEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCR 167
           + AS S       + IA+    GAD+G  E+FV + VGTP Q+F ++ADTGSDL W  CR
Sbjct: 85  ETASSS------ASSIAMPMNAGADYGVGEYFVHVTVGTPGQRFMLVADTGSDLTWMHCR 144

Query: 168 YRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDC 227
                  C      HK R   R  F    +A++SSSF  IPC S+ C  + + L     C
Sbjct: 145 -------CGRRCGTHKGRLNNRRVF----HADRSSSFKTIPCLSEMCKVELANLFSLSKC 204

Query: 228 PTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEM--TDSQFLD 287
           PTP TPC+Y Y YL G  A+G FA ET++VRL NGK+++L+D+L GCTE +   +     
Sbjct: 205 PTPLTPCAYDYRYLEGSSAIGFFANETISVRLANGKKRKLRDVLVGCTESVQGAEESGFK 264

Query: 288 GADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTS 347
           GADG++GLG   ++F  KAA+   GG FSYCL DHL      +Y +FG    K   AS S
Sbjct: 265 GADGVLGLGFGNHTFTRKAAQ-YFGGKFSYCLVDHLSPKNLSNYIIFG--HDKADKASCS 324

Query: 348 SPIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTM 407
           S +     T L  GG Y  +YGV LSGIS+ G +L IP   WN   G G IL++GTSLT 
Sbjct: 325 SSL---QHTDLVLGGDYGPFYGVNLSGISIGGVLLRIPSVAWNASLGGGAILESGTSLTF 384

Query: 408 LTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGA 467
           LT P +  V   +     +FG +     G     F+ CFN T ++   +P L  HF +GA
Sbjct: 385 LTDPVYGPVTSELNKFTSRFGTLLPPGGGP----FEFCFNSTGYDESKMPPLRIHFSNGA 444

Query: 468 VFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           +FEPP +SYI+  + +  C+   S  +P  +I+GNI+QQ  +W++DL    + FAPS C
Sbjct: 445 IFEPPVKSYILDIAPEKKCLGFVSASWPGTSIIGNIMQQNHLWEFDLENTRLGFAPSTC 463

BLAST of CmaCh02G006910 vs. TAIR10
Match: AT3G12700.1 (AT3G12700.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 273.5 bits (698), Expect = 2.8e-73
Identity = 164/466 (35.19%), Postives = 241/466 (51.72%), Query Frame = 1

Query: 60  VKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHS 119
           +K  H +  + K   RI+D+   DQ R   IS   N T         VK   GS      
Sbjct: 51  LKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTV-------GVKMDLGS------ 110

Query: 120 QTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPS 179
                     G D+G++++F +++VGTP +KF ++ DTGS+L W  CRYR          
Sbjct: 111 ----------GIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRYRA--------- 170

Query: 180 PIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYS 239
                R K   R      A++S SF  + C ++ C  D   L     CPTP+TPCSY Y 
Sbjct: 171 -----RGKDNRR---VFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYR 230

Query: 240 YLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYS 299
           Y  G  A G+FA ET+TV LTNG+  +L   L GC+   T   F  GADG++GL  S +S
Sbjct: 231 YADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSF-QGADGVLGLAFSDFS 290

Query: 300 FVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTG 359
           F    A +  G  FSYCL DHL N    +Y +FG+      +   ++P+           
Sbjct: 291 FT-STATSLYGAKFSYCLVDHLSNKNVSNYLIFGSSRSTKTAFRRTTPLDLT-------- 350

Query: 360 GRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMA 419
            R   +Y + + GIS+   +L+IP  VW+  SG GTILD+GTSLT+L   A+  V+  +A
Sbjct: 351 -RIPPFYAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLA 410

Query: 420 PKIEKFGRMEKDVKGEREKNFKLCFNDTE-WNFGMLPKLGFHFEDGAVFEPPDRSYIVSA 479
             + +  R++ +         + CF+ T  +N   LP+L FH + GA FEP  +SY+V A
Sbjct: 411 RYLVELKRVKPE-----GVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDA 460

Query: 480 SYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           +    C+   S   P+ N++GNI+QQ ++W++DL+  +++FAPS C
Sbjct: 471 APGVKCLGFVSAGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of CmaCh02G006910 vs. TAIR10
Match: AT3G25700.1 (AT3G25700.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 202.6 bits (514), Expect = 6.0e-52
Identity = 133/411 (32.36%), Postives = 199/411 (48.42%), Query Frame = 1

Query: 130 GADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMR 189
           GA  GS ++FV L++G PPQ   +IADTGSDL+W +C    CR +CS+ SP         
Sbjct: 76  GAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKC--SACR-NCSHHSPA-------- 135

Query: 190 ERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPD-CPTPN-----TPCSYTYSYLSG 249
                  +   SS+FSP  C    C      L  +PD  P  N     + C Y Y Y  G
Sbjct: 136 ----TVFFPRHSSTFSPAHCYDPVC-----RLVPKPDRAPICNHTRIHSTCHYEYGYADG 195

Query: 250 DRAMGIFATETVTVRLTNGKEKQLKDILYGC----TEEMTDSQFLDGADGLIGLGSSIYS 309
               G+FA ET +++ ++GKE +LK + +GC    + +       +GA+G++GLG    S
Sbjct: 196 SLTSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPIS 255

Query: 310 FVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTG 369
           F  +      G  FSYCL D+  +    SY + G         +    I     T L T 
Sbjct: 256 FASQLG-RRFGNKFSYCLMDYTLSPPPTSYLIIG---------NGGDGISKLFFTPLLTN 315

Query: 370 GRYSCYYGVQLSGISVDGQILNIPPHVWNI--KSGCGTILDTGTSLTMLTAPAHDAVIEA 429
                +Y V+L  + V+G  L I P +W I      GT++D+GT+L  L  PA+ +VI A
Sbjct: 316 PLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAA 375

Query: 430 MAPKIEKFGRMEKDVKGEREKNFKLCFN--DTEWNFGMLPKLGFHFEDGAVFEPPDRSYI 489
           +        R++  +       F LC N         +LP+L F F  GAVF PP R+Y 
Sbjct: 376 VR------RRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYF 435

Query: 490 VSASYQCSCIAITSL-PFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA 526
           +    Q  C+AI S+ P    +++GN++QQ F++++D  +  + F+   CA
Sbjct: 436 IETEEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCA 450

BLAST of CmaCh02G006910 vs. TAIR10
Match: AT2G42980.1 (AT2G42980.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 173.7 bits (439), Expect = 3.0e-43
Identity = 149/492 (30.28%), Postives = 217/492 (44.11%), Query Frame = 1

Query: 55  HHPEVVK---RLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEAS 114
           H  E VK   R+  E K  +    + D++  D +R++ + A  N +K  +N + + K  S
Sbjct: 73  HTRESVKPQSRIKQETK--RTTHSVVDLQIQDLTRIKTLHARFNKSKKQKNEKVRKKITS 132

Query: 115 GSN---HPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRY 174
             +    P  S   +      G   GS E+F+ + VGTPP+ F++I DTGSDL W +C  
Sbjct: 133 DISLVGAPEVSPGKLIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQC-- 192

Query: 175 RRCRGDCSNPSPIHKMRNKMRERFNYALY-ANQSSSFSPIPCSSKQCIQDFSELGGQPD- 234
             C  DC      H+         N   Y    S+SF  I C+  +C      L   PD 
Sbjct: 193 LPCY-DC-----FHQ---------NGMFYDPKTSASFKNITCNDPRC-----SLISSPDP 252

Query: 235 ---CPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLT----NGKEKQLKDILYGCTEEMT 294
              C + N  C Y Y Y       G FA ET TV LT       E ++ ++++GC     
Sbjct: 253 PVQCESDNQSCPYFYWYGDRSNTTGDFAVETFTVNLTTTEGGSSEYKVGNMMFGCGH--W 312

Query: 295 DSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKT 354
           +     GA GL+GLG    SF     ++  G  FSYCL D   N    S  +FG      
Sbjct: 313 NRGLFSGASGLLGLGRGPLSF-SSQLQSLYGHSFSYCLVDRNSNTNVSSKLIFGEDKDLL 372

Query: 355 FSASTSSPIGPPATTKLFTGGRYS--CYYGVQLSGISVDGQILNIPPHVWNIKS--GCGT 414
              + +        T    G   S   +Y +Q+  I V G+ L+IP   WNI S    GT
Sbjct: 373 NHTNLN-------FTSFVNGKENSVETFYYIQIKSILVGGKALDIPEETWNISSDGDGGT 432

Query: 415 ILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFN--DTEWNFGM 474
           I+D+GT+L+    PA++ +    A K+++   + +D           CFN    E N   
Sbjct: 433 IIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDF-----PVLDPCFNVSGIEENNIH 492

Query: 475 LPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLL 526
           LP+LG  F DG V+  P  +  +  S    C+AI   P  + +I+GN  QQ F   YD  
Sbjct: 493 LPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKSTFSIIGNYQQQNFHILYDTK 525

BLAST of CmaCh02G006910 vs. TAIR10
Match: AT3G59080.1 (AT3G59080.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 167.5 bits (423), Expect = 2.1e-41
Identity = 151/538 (28.07%), Postives = 230/538 (42.75%), Query Frame = 1

Query: 11  FFVVFFFSPLTVAVADQSNANNL------KQESDANNEEQEFVRLDLIHRHHPEVVKRLH 70
           F  + F +P+    A  S +N+       K+ +     E + V+  L  R      K   
Sbjct: 38  FSGIDFPNPMRFGSASSSTSNDCGFSSPEKEPTKERTGENKTVKFHLKRRETTTTEKATT 97

Query: 71  DEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWT---KVVENAEEKVKEASGSNHPPHSQT 130
           + +    +E +I+D+        R +  +   T   K  +N +E V     ++       
Sbjct: 98  NSV----LELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKKNDKEVVTTTPVASSVEEQAG 157

Query: 131 PIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPI 190
            +      G   GS E+F+ + VG+PP+ F++I DTGSDL W +C    C  DC      
Sbjct: 158 QLVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQC--LPCY-DCFQQ--- 217

Query: 191 HKMRNKMRERFNYALY-ANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTP----NTPCSY 250
                      N A Y    S+S+  I C+ ++C      L   PD P P    N  C Y
Sbjct: 218 -----------NGAFYDPKASASYKNITCNDQRC-----NLVSSPDPPMPCKSDNQSCPY 277

Query: 251 TYSYLSGDRAMGIFATETVTVRL-TNGKEKQL---KDILYGCTEEMTDSQFLDGADGLIG 310
            Y Y       G FA ET TV L TNG   +L   +++++GC     +     GA GL+G
Sbjct: 278 YYWYGDSSNTTGDFAVETFTVNLTTNGGSSELYNVENMMFGCGH--WNRGLFHGAAGLLG 337

Query: 311 LGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPA 370
           LG    SF     ++  G  FSYCL D   +    S  +FG                P  
Sbjct: 338 LGRGPLSF-SSQLQSLYGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSH--------PNL 397

Query: 371 TTKLFTGGR---YSCYYGVQLSGISVDGQILNIPPHVWNIKS--GCGTILDTGTSLTMLT 430
               F  G+      +Y VQ+  I V G++LNIP   WNI S    GTI+D+GT+L+   
Sbjct: 398 NFTSFVAGKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFA 457

Query: 431 APAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVF 490
            PA++ +   +A K +    + +D           CFN +  +   LP+LG  F DGAV+
Sbjct: 458 EPAYEFIKNKIAEKAKGKYPVYRDF-----PILDPCFNVSGIHNVQLPELGIAFADGAVW 517

Query: 491 EPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA 526
             P  +  +  +    C+A+   P  + +I+GN  QQ F   YD  +  + +AP+ CA
Sbjct: 518 NFPTENSFIWLNEDLVCLAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKCA 533

BLAST of CmaCh02G006910 vs. TAIR10
Match: AT5G33340.1 (AT5G33340.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 148.7 bits (374), Expect = 1.0e-35
Identity = 113/391 (28.90%), Postives = 177/391 (45.27%), Query Frame = 1

Query: 135 SSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPIHKMRNKMRERFNY 194
           S E+ + + +GTPP     IADTGSDLLWT+C            +P      ++   F+ 
Sbjct: 87  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQC------------APCDDCYTQVDPLFD- 146

Query: 195 ALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGDRAMGIFATET 254
                 SS++  + CSS QC    + L  Q  C T +  CSY+ SY       G  A +T
Sbjct: 147 ---PKTSSTYKDVSCSSSQC----TALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 206

Query: 255 VTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKAAENNVGGGFS 314
           +T+  ++ +  QLK+I+ GC      + F     G++GLG    S + K   +++ G FS
Sbjct: 207 LTLGSSDTRPMQLKNIIIGCGHNNAGT-FNKKGSGIVGLGGGPVSLI-KQLGDSIDGKFS 266

Query: 315 YCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSCYYGVQLSGIS 374
           YCL          S   FGT +  + S   S+P+   A+ + F        Y + L  IS
Sbjct: 267 YCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETF--------YYLTLKSIS 326

Query: 375 VDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEKFGRMEKDVKG 434
           V  + +           G   I+D+GT+LT+L    +  + +A+A  I      + + K 
Sbjct: 327 VGSKQIQYSGSDSESSEG-NIIIDSGTTLTLLPTEFYSELEDAVASSI------DAEKKQ 386

Query: 435 EREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSCIAITSLPFPS 494
           + +    LC++ T      +P +  HF DGA  +    +  V  S    C A      PS
Sbjct: 387 DPQSGLSLCYSAT--GDLKVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAFRG--SPS 435

Query: 495 INILGNIIQQTFIWKYDLLKGSVTFAPSDCA 526
            +I GN+ Q  F+  YD +  +V+F P+DCA
Sbjct: 447 FSIYGNVAQMNFLVGYDTVSKTVSFKPTDCA 435

BLAST of CmaCh02G006910 vs. NCBI nr
Match: gi|659112547|ref|XP_008456273.1| (PREDICTED: aspartic proteinase CDR1 [Cucumis melo])

HSP 1 Score: 517.7 bits (1332), Expect = 2.4e-143
Identity = 270/537 (50.28%), Postives = 359/537 (66.85%), Query Frame = 1

Query: 1   MSSISHLLILFFVVFFFS---PLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHP 60
           MS IS+    F ++FF S       A+ D++N  N   + D    EQ+ +R DL+HRHHP
Sbjct: 8   MSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDED----EQQTIRFDLLHRHHP 67

Query: 61  EVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVE-------NAEEKVKEA 120
           +V ++L+ ++K+  + +R+KDI  HD++R R+IS  +N  ++ +        A  +V+ A
Sbjct: 68  QVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVA 127

Query: 121 SGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRR 180
             +  PP + TPI +K   GADFGSSE+FVQLKVGTP Q F +IADTGSDL W +CRYRR
Sbjct: 128 KSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRR 187

Query: 181 CRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTP 240
           C G+CS  +  HK +N+ ++RF +AL ANQSS+F  + CSS  C  + +EL    +C TP
Sbjct: 188 CFGNCSG-NVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTP 247

Query: 241 NTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGL 300
            +PC Y YSY  G  A GIFA ET+TV LTNGKEKQL++ + GCTE +  + F DGADG+
Sbjct: 248 TSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVF-DGADGV 307

Query: 301 IGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGP 360
           +GLG+S YS  YKAAEN  GGGFSYCL DHL +  A+SYFV G P+P T SASTSS   P
Sbjct: 308 MGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPST-SASTSS-AKP 367

Query: 361 PAT---TKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLT 420
           PA    TKL+ G  YS +YGV L GIS DGQ+LNIPP VW+   GCGTI+D+GTSLT+L 
Sbjct: 368 PAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLA 427

Query: 421 APAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVF 480
            PA D V+E +  ++++F ++E +        F  CFN++++   M PKL FHF DG VF
Sbjct: 428 TPAFDVVMEVLTSRLKQFQQIEIE-------PFNFCFNNSQYTHDMAPKLRFHFGDGTVF 487

Query: 481 EPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           EPP +SYIVS     SCI I S+PFPS+NI+GNI+QQ  +W++D  K  V FA S+C
Sbjct: 488 EPPTKSYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

BLAST of CmaCh02G006910 vs. NCBI nr
Match: gi|778713001|ref|XP_004140022.2| (PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus])

HSP 1 Score: 507.7 bits (1306), Expect = 2.5e-140
Identity = 269/539 (49.91%), Postives = 355/539 (65.86%), Query Frame = 1

Query: 1   MSSISHL--LILFFVVFFF----SPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHR 60
           MS IS+      FF++FFF    S    A+ D+ N  N     + + +EQE ++ DL+HR
Sbjct: 8   MSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEIIKFDLLHR 67

Query: 61  HHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVE-----NAEEKVKE 120
           HHP+V +++H ++K+  + +R+KDI  HD +R R+IS  +N  +V +      AE   +E
Sbjct: 68  HHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLRAEAEAATEE 127

Query: 121 --ASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCR 180
             A  +  PP + TPI ++   GADFGSSE+FV+LKVGTP Q F +IADTGSDL W +CR
Sbjct: 128 EVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSDLTWMKCR 187

Query: 181 YRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDC 240
           YRRC G+CS+ +  HK +N+ ++RF +A  AN SSSF  + CSS  C  D ++L    +C
Sbjct: 188 YRRCFGNCSS-NVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLADLFAVREC 247

Query: 241 PTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGA 300
             P +PC Y YSY  G  A GIFA ET+TV LTNGKEKQL + + GCTE +  S F  GA
Sbjct: 248 HNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQGSVF-GGA 307

Query: 301 DGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSP 360
           DG++GLG+S YS  YKAAEN  GGGFSYCL DHL +  AISYFV G P+P T SASTSS 
Sbjct: 308 DGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPST-SASTSSA 367

Query: 361 IGPP--ATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTM 420
             P     TKL+ G  YS +YGV L GIS +G +LNIP  VW+I SG GTI+D+GTSLT+
Sbjct: 368 KLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIIDSGTSLTI 427

Query: 421 LTAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGA 480
           L APA D V+EA+ P+++KF ++E +        F  CFN++++   M PKL FHF DG 
Sbjct: 428 LAAPAFDMVMEALTPRLKKFQQLEIE-------PFDFCFNNSQYTHEMAPKLRFHFGDGT 487

Query: 481 VFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           VFEPP +SYIVS     SCI   S+PFP+ NI+GNI+QQ  +W++D  K  V FAPS+C
Sbjct: 488 VFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSEC 536

BLAST of CmaCh02G006910 vs. NCBI nr
Match: gi|147814824|emb|CAN65806.1| (hypothetical protein VITISV_015630 [Vitis vinifera])

HSP 1 Score: 342.4 bits (877), Expect = 1.4e-90
Identity = 190/478 (39.75%), Postives = 269/478 (56.28%), Query Frame = 1

Query: 47  VRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEK 106
           +RL+LIHRH P+V+ R   +++      R+K++ + D  R   I   L   ++      K
Sbjct: 1   MRLELIHRHSPQVMGRPKTQLQ------RLKELVHSDSVRQLMILHKLRGGQI---PRRK 60

Query: 107 VKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRC 166
            KE   S+    S   I +  +P AD+G  ++FV  KVGTP QKF ++ADTGSDL W  C
Sbjct: 61  AKEVLSSSSGRGSDDAIEVPMHPAADYGIGQYFVAFKVGTPSQKFMLVADTGSDLTWMSC 120

Query: 167 RYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPD 226
           +Y     +CSN       R   R R     +AN SSSF  IPC +  C  +  +L    +
Sbjct: 121 KYHCRSRNCSN-------RKARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTN 180

Query: 227 CPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDG 286
           CPTP TPC Y Y Y  G  A+G FA ETVTV L  G++ +L ++L GC+E      F   
Sbjct: 181 CPTPLTPCGYDYRYSDGSTALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSF-QA 240

Query: 287 ADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSS 346
           ADG++GLG S YSF  KAAE   GG FSYCL DHL +    +Y  FG+      S S  +
Sbjct: 241 ADGVMGLGYSKYSFAIKAAE-KFGGKFSYCLVDHLSHKNVSNYLTFGS------SRSKEA 300

Query: 347 PIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTML 406
            +     T+L   G  + +Y V + GIS+ G +L IP  VW++K   GTILD+G+SLT L
Sbjct: 301 LLNNMTYTELVL-GMVNSFYAVNMMGISIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFL 360

Query: 407 TAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAV 466
           T PA+  V+ A+   + KF ++E D+        + CFN T +   ++P+L FHF DGA 
Sbjct: 361 TEPAYQPVMAALRVSLLKFRKVEMDI-----GPLEYCFNSTGFEESLVPRLVFHFADGAE 420

Query: 467 FEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           FEPP +SY++SA+    C+   S+ +P  +++GNI+QQ  +W++DL    + FAPS C
Sbjct: 421 FEPPVKSYVISAADGVRCLGFVSVAWPGTSVVGNIMQQNHLWEFDLGLKKLGFAPSSC 448

BLAST of CmaCh02G006910 vs. NCBI nr
Match: gi|731434480|ref|XP_002265771.3| (PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera])

HSP 1 Score: 339.3 bits (869), Expect = 1.2e-89
Identity = 189/478 (39.54%), Postives = 268/478 (56.07%), Query Frame = 1

Query: 47  VRLDLIHRHHPEVVKRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEK 106
           +RL+LIHRH P+V+ R   +++      R+K++ + D  R   I   L   ++      K
Sbjct: 41  MRLELIHRHSPQVMGRPKTQLQ------RLKELVHSDSVRQLMILHKLRGGQI---PRRK 100

Query: 107 VKEASGSNHPPHSQTPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRC 166
            KE   S+    S   I +  +P AD+G  ++ V  KVGTP QKF ++ADTGSDL W  C
Sbjct: 101 AKEVLSSSSGRGSDDAIEVPMHPAADYGIGQYSVAFKVGTPSQKFMLVADTGSDLTWMSC 160

Query: 167 RYRRCRGDCSNPSPIHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPD 226
           +Y     +CSN       R   R R     +AN SSSF  IPC +  C  +  +L    +
Sbjct: 161 KYHCRSRNCSN-------RKARRIRHKRVFHANLSSSFKTIPCLTDMCKIELMDLFSLTN 220

Query: 227 CPTPNTPCSYTYSYLSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDG 286
           CPTP TPC Y Y Y  G  A+G FA ETVTV L  G++ +L ++L GC+E      F   
Sbjct: 221 CPTPLTPCGYDYRYSDGSTALGFFANETVTVELKEGRKMKLHNVLIGCSESFQGQSF-QA 280

Query: 287 ADGLIGLGSSIYSFVYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSS 346
           ADG++GLG S YSF  KAAE   GG FSYCL DHL +    +Y  FG+      S S  +
Sbjct: 281 ADGVMGLGYSKYSFAIKAAE-KFGGKFSYCLVDHLSHKNVSNYLTFGS------SRSKEA 340

Query: 347 PIGPPATTKLFTGGRYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTML 406
            +     T+L   G  + +Y V + GIS+ G +L IP  VW++K   GTILD+G+SLT L
Sbjct: 341 LLNNMTYTELVL-GMVNSFYAVNMMGISIGGAMLKIPSEVWDVKGAGGTILDSGSSLTFL 400

Query: 407 TAPAHDAVIEAMAPKIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAV 466
           T PA+  V+ A+   + KF ++E D+        + CFN T +   ++P+L FHF DGA 
Sbjct: 401 TEPAYQPVMAALRVSLLKFRKVEMDI-----GPLEYCFNSTGFEESLVPRLVFHFADGAE 460

Query: 467 FEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           FEPP +SY++SA+    C+   S+ +P  +++GNI+QQ  +W++DL    + FAPS C
Sbjct: 461 FEPPVKSYVISAADGVRCLGFVSVAWPGTSVVGNIMQQNHLWEFDLGLKKLGFAPSSC 488

BLAST of CmaCh02G006910 vs. NCBI nr
Match: gi|728835766|gb|KHG15209.1| (Asparticase nepenthesin-1 [Gossypium arboreum])

HSP 1 Score: 325.1 bits (832), Expect = 2.3e-85
Identity = 193/520 (37.12%), Postives = 279/520 (53.65%), Query Frame = 1

Query: 7   LLILFFVVFFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRLHDE 66
           +L+ F V+F        V  Q + + ++ + D+N+     + L+LIHRH P+        
Sbjct: 7   ILVPFMVLFSM------VVAQQHVDQMQHQHDSNS-----ITLELIHRHAPQFTNN---- 66

Query: 67  IKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQTPIALK 126
                   R+ D+ YHD  R   I +H    K  +     +K             P+A  
Sbjct: 67  -NPITQHQRLVDLLYHDIIR-HGIMSHRRRAKEEDPLTASIK------------MPLA-- 126

Query: 127 TYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGD--CSNPSPIHKM 186
              G DFG  ++    KVGTP QKF +I DTGSDL W RCRYR  RGD  C++   I++ 
Sbjct: 127 --SGRDFGIGQYITSFKVGTPSQKFWLIVDTGSDLTWIRCRYRCSRGDRSCTSKGRINRK 186

Query: 187 RNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSYLSGD 246
           R           +A  SSSF+P+PC S+ C  +   L     CPTP TPC+Y Y Y  G 
Sbjct: 187 R---------VFHAPLSSSFNPVPCFSEMCKVELMNLFSLTTCPTPITPCAYDYRYSDGS 246

Query: 247 RAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSFVYKA 306
            AMG+FA ETV+  LTNG++ +L ++L GCT+       L   DG++GL ++ YSF   A
Sbjct: 247 AAMGVFANETVSAGLTNGRKTRLHNVLIGCTDSF-QGPTLQNVDGIMGLANTKYSFATNA 306

Query: 307 AENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGGRYSC 366
           A    GG FSYCL DHL ++ A +Y +FGT      + +     G    TKL      S 
Sbjct: 307 AA-TFGGKFSYCLVDHLSHLNATNYIIFGT------NRNQVKVSGNTRHTKLELDAIPS- 366

Query: 367 YYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIEK 426
           +Y V + GISV  ++L IP  VW+   G GTI+D+GTSLT L  PA+ AV+EA+   + K
Sbjct: 367 FYAVNVIGISVGNKMLEIPMQVWDASEGGGTIIDSGTSLTFLADPAYQAVMEALKVSVSK 426

Query: 427 FGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASYQCSC 486
           + R++ D         + CFN T +N  ++PKL  HF+DGA FEP   SY+++A+ +  C
Sbjct: 427 YQRVKLD-----GVPMEYCFNSTGFNGSLVPKLIIHFDDGARFEPHWNSYVIAAAAEVRC 470

Query: 487 IAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDC 525
           +      FP+++++GNI+QQ ++W++DL    + FAPS C
Sbjct: 487 LGFLPARFPALSVIGNIMQQNYLWEFDLKGKRLVFAPSSC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NEP2_NEPGR1.3e-3731.74Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
CDR1_ARATH1.8e-3428.90Aspartic proteinase CDR1 OS=Arabidopsis thaliana GN=CDR1 PE=1 SV=1[more]
NEP1_NEPGR1.7e-3230.15Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
APF2_ARATH1.9e-3130.25Aspartyl protease family protein 2 OS=Arabidopsis thaliana GN=APF2 PE=2 SV=1[more]
ASPG1_ARATH1.5e-2825.61Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana GN=ASPG1 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KG92_CUCSA1.7e-14049.91Uncharacterized protein OS=Cucumis sativus GN=Csa_6G134390 PE=3 SV=1[more]
A5BLS9_VITVI9.6e-9139.75Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_015630 PE=3 SV=1[more]
F6H9S0_VITVI8.1e-9039.54Putative uncharacterized protein OS=Vitis vinifera GN=VIT_19s0085g01110 PE=3 SV=... [more]
A0A0B0NTS3_GOSAR1.6e-8537.12Asparticase nepenthesin-1 OS=Gossypium arboreum GN=F383_00615 PE=3 SV=1[more]
W9QQY3_9ROSA2.7e-8539.67Aspartic proteinase nepenthesin-1 OS=Morus notabilis GN=L484_019203 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G12700.12.8e-7335.19 Eukaryotic aspartyl protease family protein[more]
AT3G25700.16.0e-5232.36 Eukaryotic aspartyl protease family protein[more]
AT2G42980.13.0e-4330.28 Eukaryotic aspartyl protease family protein[more]
AT3G59080.12.1e-4128.07 Eukaryotic aspartyl protease family protein[more]
AT5G33340.11.0e-3528.90 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|659112547|ref|XP_008456273.1|2.4e-14350.28PREDICTED: aspartic proteinase CDR1 [Cucumis melo][more]
gi|778713001|ref|XP_004140022.2|2.5e-14049.91PREDICTED: protein ASPARTIC PROTEASE IN GUARD CELL 2 [Cucumis sativus][more]
gi|147814824|emb|CAN65806.1|1.4e-9039.75hypothetical protein VITISV_015630 [Vitis vinifera][more]
gi|731434480|ref|XP_002265771.3|1.2e-8939.54PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera][more]
gi|728835766|gb|KHG15209.1|2.3e-8537.12Asparticase nepenthesin-1 [Gossypium arboreum][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0044238 primary metabolic process
biological_process GO:0030163 protein catabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G006910.1CmaCh02G006910.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 144..164
score: 3.0E-9coord: 496..511
score: 3.0E-9coord: 395..406
score: 3.
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 124..524
score: 6.0E-111coord: 1..106
score: 6.0E
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 135..168
score: 6.8E-35coord: 201..333
score: 6.8E-35coord: 354..525
score: 1.6
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 130..524
score: 7.15
NoneNo IPR availableunknownCoilCoilcoord: 25..45
scor
NoneNo IPR availablePANTHERPTHR13683:SF280ASPARTYL PROTEASE FAMILY PROTEINcoord: 124..524
score: 6.0E-111coord: 1..106
score: 6.0E

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh02G006910CmaCh20G002930Cucurbita maxima (Rimu)cmacmaB482