CmaCh02G004540 (gene) Cucurbita maxima (Rimu)

NameCmaCh02G004540
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionInositol-tetrakisphosphate 1-kinase
LocationCma_Chr02 : 2357176 .. 2358279 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTTCTTCTTCATCGTCGTCGTCGTCGTCGTCGTCGGATGGCTTTCGTGTAGGCTATGCTTTTCCCCCAAACAAAGAGCGTAATGTGATTCGTCCATCTCTGATTGACTACGCAAAGCTCCATGGCGTCGACCTGGTCCGCATCGATCTTCAAACGCCGATTCTCCACCAAGGTCCGTTTCACTGTATCATACACAAGATGTACGACGATGCTTGGGTGGATACACTTCAGGATTTCGCCTCGAAGAATCCGGACGTTGTTGTTGTGGATCGGCCGGACCGGATCGAGCGACTCTACAACAGAGTATCGATGCTGGATGTGGTGAATCAGGTGAAGGTTTCAGACAGCGGCGTGAAGATCGAGGTGCCGAAACAGGTCGTTTTGAATCGGAAGGATGGAGTAATCGATTTGATTAGGGATTTGGAGGTGAAATTTCCAGTGATTGCGAAGCCGTTGGAGTCGAATGGAAGCGCGAAATCGCACGAAATGTCTCTGGTTTACAATCGACGCGGATTCAAGGATTTGGAAACGCCAGTGCTTCTGCAGGAATTTGTGAACCACGGCGGGGTAATGTTCAAGGTTTACGTCGCGGGAGAGCAATCGGTGTGCGTGAAGAGGAAATCGCTGCCGGATGTGGTGGAAACAGAGGAGGAATTGGAGAAAAAAACAGACGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAAAAGAGCGAAAAATGCAATTGCGGCGGAAAGAAGGAGGCGGCGGCGGAGGAGGAGATCGAAATGCCGCCGGAGAAGGTAGTGACGGAGGTGTCGAGAGGGCTGAGGGAAGCCATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGATCGGAATGGAACGTACTACGTGATCGACATAAATTACTTACCAGGATTTGCAGTGCTGCCGGATTACGAGGCATTTCTGACGAAATTCTTCAACGAAGTTATGCAGAAGAAGGTGGTGGAAGAGGAGAATTCCGCCATGGAAGACGAGAAAGCAAAACCACGGTGCTGCTGTTTCCATGAACGTGAAAGTCAAGCCTCCACGTTTGAAAACGACTTTTGTTCACGTGTGCTGAATTAG

mRNA sequence

ATGGCTTCTTCTTCTTCATCGTCGTCGTCGTCGTCGTCGTCGGATGGCTTTCGTGTAGGCTATGCTTTTCCCCCAAACAAAGAGCGTAATGTGATTCGTCCATCTCTGATTGACTACGCAAAGCTCCATGGCGTCGACCTGGTCCGCATCGATCTTCAAACGCCGATTCTCCACCAAGGTCCGTTTCACTGTATCATACACAAGATGTACGACGATGCTTGGGTGGATACACTTCAGGATTTCGCCTCGAAGAATCCGGACGTTGTTGTTGTGGATCGGCCGGACCGGATCGAGCGACTCTACAACAGAGTATCGATGCTGGATGTGGTGAATCAGGTGAAGGTTTCAGACAGCGGCGTGAAGATCGAGGTGCCGAAACAGGTCGTTTTGAATCGGAAGGATGGAGTAATCGATTTGATTAGGGATTTGGAGGTGAAATTTCCAGTGATTGCGAAGCCGTTGGAGTCGAATGGAAGCGCGAAATCGCACGAAATGTCTCTGGTTTACAATCGACGCGGATTCAAGGATTTGGAAACGCCAGTGCTTCTGCAGGAATTTGTGAACCACGGCGGGGTAATGTTCAAGGTTTACGTCGCGGGAGAGCAATCGGTGTGCGTGAAGAGGAAATCGCTGCCGGATGTGGTGGAAACAGAGGAGGAATTGGAGAAAAAAACAGACGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAAAAGAGCGAAAAATGCAATTGCGGCGGAAAGAAGGAGGCGGCGGCGGAGGAGGAGATCGAAATGCCGCCGGAGAAGGTAGTGACGGAGGTGTCGAGAGGGCTGAGGGAAGCCATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGATCGGAATGGAACGTACTACGTGATCGACATAAATTACTTACCAGGATTTGCAGTGCTGCCGGATTACGAGGCATTTCTGACGAAATTCTTCAACGAAGTTATGCAGAAGAAGGTGGTGGAAGAGGAGAATTCCGCCATGGAAGACGAGAAAGCAAAACCACGGTGCTGCTGTTTCCATGAACGTGAAAGTCAAGCCTCCACGTTTGAAAACGACTTTTGTTCACGTGTGCTGAATTAG

Coding sequence (CDS)

ATGGCTTCTTCTTCTTCATCGTCGTCGTCGTCGTCGTCGTCGGATGGCTTTCGTGTAGGCTATGCTTTTCCCCCAAACAAAGAGCGTAATGTGATTCGTCCATCTCTGATTGACTACGCAAAGCTCCATGGCGTCGACCTGGTCCGCATCGATCTTCAAACGCCGATTCTCCACCAAGGTCCGTTTCACTGTATCATACACAAGATGTACGACGATGCTTGGGTGGATACACTTCAGGATTTCGCCTCGAAGAATCCGGACGTTGTTGTTGTGGATCGGCCGGACCGGATCGAGCGACTCTACAACAGAGTATCGATGCTGGATGTGGTGAATCAGGTGAAGGTTTCAGACAGCGGCGTGAAGATCGAGGTGCCGAAACAGGTCGTTTTGAATCGGAAGGATGGAGTAATCGATTTGATTAGGGATTTGGAGGTGAAATTTCCAGTGATTGCGAAGCCGTTGGAGTCGAATGGAAGCGCGAAATCGCACGAAATGTCTCTGGTTTACAATCGACGCGGATTCAAGGATTTGGAAACGCCAGTGCTTCTGCAGGAATTTGTGAACCACGGCGGGGTAATGTTCAAGGTTTACGTCGCGGGAGAGCAATCGGTGTGCGTGAAGAGGAAATCGCTGCCGGATGTGGTGGAAACAGAGGAGGAATTGGAGAAAAAAACAGACGGCGCGATGAAGTTTTCGCAGATATCTAGGGCAGAGGAAAAGAGCGAAAAATGCAATTGCGGCGGAAAGAAGGAGGCGGCGGCGGAGGAGGAGATCGAAATGCCGCCGGAGAAGGTAGTGACGGAGGTGTCGAGAGGGCTGAGGGAAGCCATGGGGATTAGGCTGTTCAATTTCGACATGATTAGGGATCGGAATGGAACGTACTACGTGATCGACATAAATTACTTACCAGGATTTGCAGTGCTGCCGGATTACGAGGCATTTCTGACGAAATTCTTCAACGAAGTTATGCAGAAGAAGGTGGTGGAAGAGGAGAATTCCGCCATGGAAGACGAGAAAGCAAAACCACGGTGCTGCTGTTTCCATGAACGTGAAAGTCAAGCCTCCACGTTTGAAAACGACTTTTGTTCACGTGTGCTGAATTAG

Protein sequence

MASSSSSSSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAVLPDYEAFLTKFFNEVMQKKVVEEENSAMEDEKAKPRCCCFHERESQASTFENDFCSRVLN
BLAST of CmaCh02G004540 vs. Swiss-Prot
Match: ITPK1_ARATH (Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana GN=ITPK1 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.9e-73
Identity = 151/321 (47.04%), Postives = 217/321 (67.60%), Query Frame = 1

Query: 10  SSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKM 69
           S S  + + VGYA    K+ + I+PSLI++++  G+DLV++D    +L QG   CIIHK+
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 70  YDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVK--VSDSGVKIEVPKQ 129
           YD  W + L +F  K P V V+D P+ IERL+NRVSML+V+ Q++  VSDS  +  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQ 121

Query: 130 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 189
           VV+     +       E+KFPVIAKPL+++GSAKSH+M L+Y++ G K L+ P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 190 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 249
           NHGGV+FKVYV G+   CVKR+SLPD+    EE    + G++ FSQIS    + +K    
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEY 241

Query: 250 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRN--GTYYVIDINYLPGF 309
           G  E  + E++EMPP   +T++++ +RE+MG+ LFNFD+IRD      Y +IDINY PG+
Sbjct: 242 G--EDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGY 301

Query: 310 AVLPDYEAFLTKFFNEVMQKK 327
           A +P YE  LT+FF +++ KK
Sbjct: 302 AKMPSYEPVLTEFFWDMVTKK 316

BLAST of CmaCh02G004540 vs. Swiss-Prot
Match: ITPK1_MAIZE (Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays GN=ITPK1 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.1e-68
Identity = 151/342 (44.15%), Postives = 212/342 (61.99%), Query Frame = 1

Query: 1   MASSSSSSSSSSSSDGFR--VGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILH 60
           MAS +++  SS  +   R  +GYA  P K+++ I+PSL+  A   G+DLV +D   P+  
Sbjct: 1   MASDAAAEPSSGVTHPPRYVIGYALAPKKQQSFIQPSLVAQAASRGMDLVPVDASQPLAE 60

Query: 61  QGPFHCIIHKMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVK-VSD 120
           QGPFH +IHK+Y D W   L  FA+++P V +VD P  I+RL+NR+SML VV+++   +D
Sbjct: 61  QGPFHLLIHKLYGDDWRAQLVAFAARHPAVPIVDPPHAIDRLHNRISMLQVVSELDHAAD 120

Query: 121 SGVKIEVPKQVVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDL 180
                 +P QVV+     + D      ++FP+IAKPL ++G+AKSH+MSLVY+R G   L
Sbjct: 121 QDSTFGIPSQVVVYDAAALADFGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLGKL 180

Query: 181 ETPVLLQEFVNHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRA 240
             P++LQEFVNHGGV+FKVYV G    CVKR+SLPDV     E +    G++ FSQ+S  
Sbjct: 181 RPPLVLQEFVNHGGVIFKVYVVGGHVTCVKRRSLPDV---SPEDDASAQGSVSFSQVSNL 240

Query: 241 EEKSEKCNCGGKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRD--RNGTYY 300
             +       G+K     E+  +PP   + +++ GLR A+G++LFNFDMIRD      Y 
Sbjct: 241 PTERTAEEYYGEKSL---EDAVVPPAAFINQIAGGLRRALGLQLFNFDMIRDVRAGDRYL 300

Query: 301 VIDINYLPGFAVLPDYEAFLTKFFNEVMQKKVV---EEENSA 335
           VIDINY PG+A +P YE  LT FF E++ K  V   +EE  A
Sbjct: 301 VIDINYFPGYAKMPGYETVLTDFFWEMVHKDGVGNQQEEKGA 336

BLAST of CmaCh02G004540 vs. Swiss-Prot
Match: ITPK5_ORYSJ (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica GN=ITPK5 PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-66
Identity = 139/314 (44.27%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 17  FRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVD 76
           + +GYA  P K+++ I+PSL+  A   G+DLV +D   P+  QGPFH +IHK+Y + W  
Sbjct: 16  YLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWRG 75

Query: 77  TLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKV---SDSGVKIEVPKQVVLNRK 136
            L  F++ +P V VVD P  I+RL+NR+SML VV+++ V   +       +P QVV+   
Sbjct: 76  QLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYDA 135

Query: 137 DGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVM 196
             + D      ++FP+IAKPL ++G+AKSH+MSLVY+R G + L  P++LQEFVNHGGV+
Sbjct: 136 AALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGVI 195

Query: 197 FKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAA 256
           FKVYV G    CVKR+SLPD V ++   +   +G++ FSQ+S     +E+       +  
Sbjct: 196 FKVYVVGAHVTCVKRRSLPD-VSSDVLQDASAEGSLSFSQVSNL--PNERTAQEYYDDMR 255

Query: 257 AEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNG--TYYVIDINYLPGFAVLPDY 316
            E+ I MPP   + +++  LR A+G+ LFNFDMIRD      Y VIDINY PG+A +P Y
Sbjct: 256 LEDAI-MPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGY 315

Query: 317 EAFLTKFFNEVMQK 326
           E  LT FF E++ K
Sbjct: 316 ETVLTDFFWEMVHK 325

BLAST of CmaCh02G004540 vs. Swiss-Prot
Match: ITPK5_ORYSI (Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica GN=ITPK5 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.6e-66
Identity = 139/314 (44.27%), Postives = 199/314 (63.38%), Query Frame = 1

Query: 17  FRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVD 76
           + +GYA  P K+++ I+PSL+  A   G+DLV +D   P+  QGPFH +IHK+Y + W  
Sbjct: 16  YLIGYALAPKKQQSFIQPSLVSRAAGRGMDLVPVDPSRPLPEQGPFHLLIHKLYGEEWRG 75

Query: 77  TLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKV---SDSGVKIEVPKQVVLNRK 136
            L  F++ +P V VVD P  I+RL+NR+SML VV+++ V   +       +P QVV+   
Sbjct: 76  QLDAFSAAHPAVPVVDPPHAIDRLHNRISMLQVVSELDVPLHAHHHHTFGIPSQVVVYDA 135

Query: 137 DGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVM 196
             + D      ++FP+IAKPL ++G+AKSH+MSLVY+R G + L  P++LQEFVNHGGV+
Sbjct: 136 AALSDSGLLAALRFPLIAKPLVADGTAKSHKMSLVYHREGLRKLRPPLVLQEFVNHGGVI 195

Query: 197 FKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAA 256
           FKVYV G    CVKR+SLPD V ++   +   +G++ FSQ+S     +E+       +  
Sbjct: 196 FKVYVVGAHVTCVKRRSLPD-VSSDVLQDASAEGSLSFSQVSNL--PNERTAQEYYDDMR 255

Query: 257 AEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNG--TYYVIDINYLPGFAVLPDY 316
            E+ I MPP   + +++  LR A+G+ LFNFDMIRD      Y VIDINY PG+A +P Y
Sbjct: 256 LEDAI-MPPTAFINDIAAALRRALGLHLFNFDMIRDARAGDRYLVIDINYFPGYAKMPGY 315

Query: 317 EAFLTKFFNEVMQK 326
           E  LT FF E++ K
Sbjct: 316 ETVLTDFFWEMVHK 325

BLAST of CmaCh02G004540 vs. Swiss-Prot
Match: ITPK4_ORYSJ (Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica GN=ITPK4 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.9e-63
Identity = 146/323 (45.20%), Postives = 205/323 (63.47%), Query Frame = 1

Query: 9   SSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHK 68
           SS SSS  + VGYA  P K  +V+RPSL+  A   GV LV +D+  P+  QGPF  ++HK
Sbjct: 6   SSPSSSPRYTVGYALLPEKVSSVVRPSLVALAADRGVRLVAVDVSRPLAEQGPFDLLVHK 65

Query: 69  MYDDAWVDTLQDFASKNPDV-VVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 128
           MYD  W   L++ A+++P V VVVD P  I+RL +R +MLDVV+ ++       + VP Q
Sbjct: 66  MYDRGWRAQLEELAARHPGVTVVVDSPGAIDRLLDRATMLDVVSGLRTP-----VSVPPQ 125

Query: 129 VVLNRKDGVID-LIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDL-ETPVLLQE 188
           VV++      D L+    ++FP+IAKPL  +GSA+SH+M LVY R G   L   P++LQE
Sbjct: 126 VVVSDAAADADELLARAALRFPLIAKPLAVDGSAESHDMRLVYRRDGVLPLLRAPLVLQE 185

Query: 189 FVNHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCN 248
           FVNHGGV+FKVYV G+++ CV+R SLPD V     L+   + ++ F+ IS  +      +
Sbjct: 186 FVNHGGVLFKVYVVGDRATCVRRSSLPD-VPARRLLDLDAEPSVPFANISN-QPLPPPDD 245

Query: 249 CGGKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRN----GTYYVIDINY 308
            GG    AA++  + P    V EV+RGLR  +G+ LFNFDMIR+R+      Y++IDINY
Sbjct: 246 DGG----AADD--DTPAAGFVDEVARGLRRGLGLHLFNFDMIRERSEEHGDRYFIIDINY 305

Query: 309 LPGFAVLPDYEAFLTKFFNEVMQ 325
            PG+A +P YEA LT FF E+++
Sbjct: 306 FPGYAKMPGYEAALTDFFLEMLR 315

BLAST of CmaCh02G004540 vs. TrEMBL
Match: A0A0A0LRI5_CUCSA (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439740 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 1.8e-149
Identity = 276/344 (80.23%), Postives = 306/344 (88.95%), Query Frame = 1

Query: 8   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIH 67
           +S+S SS+  RVGYAFPPNKERNVIRPSLIDYAKLH VDLVRIDLQTP+LHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 68  KMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 127
           K+YDDAW + LQ+FASKNPDVVVVDRPD I +LYNRVSMLDVV+QVKVSDS VKIEVPKQ
Sbjct: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121

Query: 128 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 187
           +++ ++D  ID I  LE+KFPVIAKP+ES+GSAKSHEMSLV+NRRG KDL  PVL+QEFV
Sbjct: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181

Query: 188 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 247
           NHGGVMFK+YVAG+QS+CVKRKSLPDV ETEEELEKKT+GAMKFSQISRAEEKSEKCN  
Sbjct: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 248 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR-NGTYYVIDINYLPGFA 307
            KKE   EEEIEMPPEK+V EVSRGL+EAMGIRLFNFDMIRDR NG YYVIDINYLPGFA
Sbjct: 242 AKKE--DEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFA 301

Query: 308 VLPDYEAFLTKFFNEVMQKKV-VEEENSAMEDEKAKPRCC-CFH 349
           VLP+YE FLTKFF EV +KKV V+E  SA E++K  PRCC CFH
Sbjct: 302 VLPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

BLAST of CmaCh02G004540 vs. TrEMBL
Match: A0A0A0LTT9_CUCSA (Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439240 PE=3 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.1e-88
Identity = 182/322 (56.52%), Postives = 219/322 (68.01%), Query Frame = 1

Query: 13  SSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDD 72
           SS  FR+GYA    KER+ I PSL+DYAKLHG+D VRID   P+  Q PFHCIIHK+YD 
Sbjct: 2   SSPRFRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 73  AWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNR 132
           +WV  LQDF S+ PDVVVVD P+ I RL NR SML+VV +VKV     +IE PKQVV+N 
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 133 KDGV----IDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVN 192
            D V    ++   DL +KFP+IAKPLESNGSAKSH++ LV N  G K L  P++LQEFVN
Sbjct: 122 LDVVLKNGLNTFSDLGLKFPIIAKPLESNGSAKSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 193 HGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGG 252
           HGGV+FKVYV GE  VCV RKSLPD+     E  KK D    FSQIS +  + +      
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDI---GPEDVKKLDAVSSFSQISNSGAQGDD----- 241

Query: 253 KKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRN--GTYYVIDINYLPGFA 312
                 E  +EMP  + V  V+ GLREA G+RLFNFD+IRD N    Y VIDINYLPG+A
Sbjct: 242 ------EGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSNDHNRYLVIDINYLPGYA 301

Query: 313 VLPDYEAFLTKFFNEVMQKKVV 329
            +P+YE FLTKFF +V+Q + V
Sbjct: 302 KMPNYEPFLTKFFLDVVQNRTV 309

BLAST of CmaCh02G004540 vs. TrEMBL
Match: W9R373_9ROSA (Inositol-tetrakisphosphate 1-kinase OS=Morus notabilis GN=L484_017781 PE=3 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.8e-81
Identity = 161/346 (46.53%), Postives = 228/346 (65.90%), Query Frame = 1

Query: 17  FRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVD 76
           F VGYA  P K+++ I+ SL+  A+  G+DLVRI+ + P+  QGPF C++HK+Y D W  
Sbjct: 6   FGVGYALAPKKQQSFIQDSLVSLARSRGIDLVRIEREIPLADQGPFDCVLHKLYGDDWRR 65

Query: 77  TLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGV 136
            L+DF +KNP+ V++D P+ IERL+NR+SML VV+++K+ D      +PKQ+V+  K+ +
Sbjct: 66  QLEDFRAKNPNAVIIDYPEAIERLHNRISMLQVVSELKIEDRSETFGIPKQIVIYDKETL 125

Query: 137 IDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFKV 196
            D      +KFPVIAKPL ++GSAKSH+M+L++N  G  +L+ P++LQEFVNHGGV+FKV
Sbjct: 126 FDRPAWEGLKFPVIAKPLVADGSAKSHKMALIFNHDGLSNLKPPIVLQEFVNHGGVIFKV 185

Query: 197 YVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAEE 256
           YV GE   CVKRKSLPDV  +EEE     +G + FSQ+S         +   KK     +
Sbjct: 186 YVVGEYVKCVKRKSLPDV--SEEEKLGSLEGLLSFSQVSNLATNERTDDKYYKKMHL--D 245

Query: 257 EIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR--NGTYYVIDINYLPGFAVLPDYEAF 316
           E EMPP+  +T+++RGLR AM + LFNFD+IRD      Y +IDINY PG+A +P YE  
Sbjct: 246 ETEMPPQSFLTDIARGLRRAMKLNLFNFDVIRDARFGNRYLIIDINYFPGYAKMPGYETV 305

Query: 317 LTKFFNEVMQKKVVEEENSAMEDEKAKPRCCCFHERESQASTFEND 361
           LT FF ++M+KK  E E    E + A  R C    R+  ++ + +D
Sbjct: 306 LTDFFCDIMEKKEREVEKGLEEKDGADSRGCDKEMRKLVSNAYCSD 347

BLAST of CmaCh02G004540 vs. TrEMBL
Match: A0A067EMQ3_CITSI (Inositol-tetrakisphosphate 1-kinase OS=Citrus sinensis GN=CISIN_1g047754mg PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 5.3e-80
Identity = 166/324 (51.23%), Postives = 215/324 (66.36%), Query Frame = 1

Query: 18  RVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVDT 77
           ++GY F PNKE+ VI+PSLI  A   G+ L+RID   P+  QGPF CI+HK+Y   W   
Sbjct: 11  KIGYVFAPNKEQTVIQPSLITKATEQGIGLIRIDPDKPLTEQGPFDCIMHKLYGPDWTQQ 70

Query: 78  LQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGVI 137
           LQ F+S+NPDV ++D P+ IERL+NR SML+VV+++K+  +  K+ VP Q V++  + V 
Sbjct: 71  LQQFSSRNPDVRIIDPPESIERLHNRDSMLEVVSRLKIELNKEKVSVPNQAVVSETELVT 130

Query: 138 D--LIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFK 197
               I +L+++FP+IAKPLE+NGS KSH+M L+++  G + LE P+LLQEFVNHGG++FK
Sbjct: 131 RDMAIEELKLRFPLIAKPLEANGSDKSHQMYLIFDNEGLEVLEAPILLQEFVNHGGIIFK 190

Query: 198 VYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAE 257
           VYVAG    CVKRKSLPD+    EE  K   G + FSQIS         N     E    
Sbjct: 191 VYVAGSSVKCVKRKSLPDI---SEEKMKSLKGFLPFSQIS--------SNSVDADEEVDL 250

Query: 258 EEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRD---RNGTYYVIDINYLPGFAVLPDYE 317
           E+ EMP E  V E+SR LREA+G+ LFNFD+IRD   R+G Y VIDINYLPG+A LP YE
Sbjct: 251 EKTEMPREGFVVELSRALREALGLNLFNFDLIRDAGERDG-YLVIDINYLPGYAKLPGYE 310

Query: 318 AFLTKFFNEVMQKKVVEEENSAME 337
             L  FF  V + K VEE  S  E
Sbjct: 311 TLLMDFFLNVAKSKAVEEVTSGDE 322

BLAST of CmaCh02G004540 vs. TrEMBL
Match: V4VTN2_9ROSI (Inositol-tetrakisphosphate 1-kinase OS=Citrus clementina GN=CICLE_v10024568mg PE=3 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 9.0e-80
Identity = 166/324 (51.23%), Postives = 215/324 (66.36%), Query Frame = 1

Query: 18  RVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVDT 77
           ++GY F PNKE+ VI+PSLI  A   G+ L+RID   P+  QGPF CI+HK+Y   W   
Sbjct: 11  KIGYVFAPNKEQTVIQPSLITKATEQGIGLIRIDPDKPLTEQGPFDCIMHKLYGPDWTQQ 70

Query: 78  LQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGVI 137
           LQ F+S+NPDV ++D P+ IERL+NR SML+VV+++K+  +  K+ VP Q V++  + V 
Sbjct: 71  LQQFSSRNPDVRIIDPPESIERLHNRDSMLEVVSRLKIELNKEKVSVPNQAVVSETELVT 130

Query: 138 D--LIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFK 197
               I +L+++FP+IAKPLE+NGS KSH+M L+++  G + LE P+LLQEFVNHGG++FK
Sbjct: 131 RDMAIEELKLRFPLIAKPLEANGSDKSHQMYLIFDNEGLEVLEAPILLQEFVNHGGMIFK 190

Query: 198 VYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAE 257
           VYVAG    CVKRKSLPD+    EE  K   G + FSQIS         N     E    
Sbjct: 191 VYVAGSSVKCVKRKSLPDI---SEEKMKSLKGFLPFSQIS--------SNSVDADEEVDL 250

Query: 258 EEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRD---RNGTYYVIDINYLPGFAVLPDYE 317
           E+ EMP E  V E+SR LREA+G+ LFNFD+IRD   R+G Y VIDINYLPG+A LP YE
Sbjct: 251 EKTEMPREGFVVELSRALREALGLNLFNFDLIRDAGERDG-YLVIDINYLPGYAKLPGYE 310

Query: 318 AFLTKFFNEVMQKKVVEEENSAME 337
             L  FF  V + K VEE  S  E
Sbjct: 311 TLLMDFFLNVAKSKAVEEVTSGDE 322

BLAST of CmaCh02G004540 vs. TAIR10
Match: AT5G16760.1 (AT5G16760.1 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 275.8 bits (704), Expect = 3.9e-74
Identity = 151/321 (47.04%), Postives = 217/321 (67.60%), Query Frame = 1

Query: 10  SSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKM 69
           S S  + + VGYA    K+ + I+PSLI++++  G+DLV++D    +L QG   CIIHK+
Sbjct: 2   SDSIQERYLVGYALAAKKQHSFIQPSLIEHSRQRGIDLVKLDPTKSLLEQGKLDCIIHKL 61

Query: 70  YDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVK--VSDSGVKIEVPKQ 129
           YD  W + L +F  K P V V+D P+ IERL+NRVSML+V+ Q++  VSDS  +  VP+Q
Sbjct: 62  YDVYWKENLHEFREKCPGVPVIDLPEAIERLHNRVSMLEVITQLRFPVSDSE-RFGVPEQ 121

Query: 130 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 189
           VV+     +       E+KFPVIAKPL+++GSAKSH+M L+Y++ G K L+ P++LQEFV
Sbjct: 122 VVVMDSSVLSGGGALGELKFPVIAKPLDADGSAKSHKMFLIYDQEGMKILKAPIVLQEFV 181

Query: 190 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 249
           NHGGV+FKVYV G+   CVKR+SLPD+    EE    + G++ FSQIS    + +K    
Sbjct: 182 NHGGVIFKVYVVGDHVKCVKRRSLPDI---SEEKIGTSKGSLPFSQISNLTAQEDKNIEY 241

Query: 250 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRN--GTYYVIDINYLPGF 309
           G  E  + E++EMPP   +T++++ +RE+MG+ LFNFD+IRD      Y +IDINY PG+
Sbjct: 242 G--EDRSLEKVEMPPLSFLTDLAKAMRESMGLNLFNFDVIRDAKDANRYLIIDINYFPGY 301

Query: 310 AVLPDYEAFLTKFFNEVMQKK 327
           A +P YE  LT+FF +++ KK
Sbjct: 302 AKMPSYEPVLTEFFWDMVTKK 316

BLAST of CmaCh02G004540 vs. TAIR10
Match: AT4G33770.1 (AT4G33770.1 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 236.1 bits (601), Expect = 3.4e-62
Identity = 125/317 (39.43%), Postives = 190/317 (59.94%), Query Frame = 1

Query: 19  VGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVDTL 78
           VGYA    K+++ ++P L   A+  G+  V IDL  P+  QGPF  ++HK+    W + +
Sbjct: 83  VGYALTSKKKKSFLQPKLELLARRKGIFFVAIDLNRPLSEQGPFDVVLHKLLGKEWEEVI 142

Query: 79  QDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ-VVLNRKDGVI 138
           +D+  K+P+V V+D P  I+R+YNR SML  +  +K+SD    + VPKQ VVL       
Sbjct: 143 EDYQQKHPEVTVLDPPGSIQRIYNRQSMLQGMADLKLSDCSGSLFVPKQMVVLKDSAASA 202

Query: 139 DLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFKVY 198
           D + +  +KFP++AKPL  +G+AKSH++ L Y+RR   +L+ P++LQEFVNHGGVMFKV+
Sbjct: 203 DAVVEAGLKFPLVAKPLWIDGTAKSHQLYLAYDRRSLAELDPPLVLQEFVNHGGVMFKVF 262

Query: 199 VAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAEEE 258
           V G+    ++R SLP+V   E   + K DG  +F ++S A   ++  +   +        
Sbjct: 263 VVGDVIKVMRRFSLPNVSNCE---KAKVDGVFQFPRVSSAAASADNADLDPR-------V 322

Query: 259 IEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR--NGTYYVIDINYLPGFAVLPDYEAFL 318
            E+PP+  +  + + LR  +G+RLFN DMIR+      +YVIDINY PG+  LPDYE   
Sbjct: 323 AELPPKPFLEALVKELRSLLGLRLFNIDMIREHGSKNVFYVIDINYFPGYGKLPDYEQVF 382

Query: 319 TKFFNEVMQKKVVEEEN 333
             FF  + Q K  + ++
Sbjct: 383 VDFFQNLAQVKYKKRQH 389

BLAST of CmaCh02G004540 vs. TAIR10
Match: AT4G08170.2 (AT4G08170.2 Inositol 1,3,4-trisphosphate 5/6-kinase family protein)

HSP 1 Score: 226.5 bits (576), Expect = 2.7e-59
Identity = 127/313 (40.58%), Postives = 187/313 (59.74%), Query Frame = 1

Query: 19  VGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVDTL 78
           VGYA    K ++ ++P L   A+  G+  V ID   P+  QGPF  ++HK     W   L
Sbjct: 43  VGYALTSKKIKSFLQPKLEGLARNKGILFVAIDQNKPLSEQGPFDIVLHKQIGKEWRRIL 102

Query: 79  QDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGVI- 138
           ++F   +PDV V+D PD I  L NR SML  V  + +SDS  ++ VPKQ+V+ +    I 
Sbjct: 103 EEFRLAHPDVTVLDPPDAILHLRNRQSMLQCVADMNLSDSNGRVGVPKQLVIKKDASSIP 162

Query: 139 DLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFKVY 198
           + + +  ++ P++AKPL ++GSAKSHE+SL Y++     LE P++LQEFVNHGGV+FKVY
Sbjct: 163 EAVNNAGLRLPLVAKPLVADGSAKSHELSLAYDQHSLLKLEPPLVLQEFVNHGGVLFKVY 222

Query: 199 VAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAEEE 258
           + GE    V+R SLPDV  +  EL K   G  +F ++S        C      +A  +  
Sbjct: 223 IVGEAIRVVRRFSLPDV--SRRELPKSA-GVFRFPRVS--------CAAASADDADLDPS 282

Query: 259 I-EMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGT---YYVIDINYLPGFAVLPDYEA 318
           I E+PP  ++  +++ LR  +G+RLFN D+IR+ +GT   +YVIDINY PG+  +P+YE 
Sbjct: 283 IAELPPRPLLERLAKELRRGLGLRLFNLDIIRE-HGTRDRFYVIDINYFPGYGKMPEYEH 342

Query: 319 FLTKFFNEVMQKK 327
             T F   V+Q +
Sbjct: 343 VFTDFLLSVVQSQ 343

BLAST of CmaCh02G004540 vs. TAIR10
Match: AT2G43980.1 (AT2G43980.1 inositol 1,3,4-trisphosphate 5/6-kinase 4)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-16
Identity = 60/201 (29.85%), Postives = 100/201 (49.75%), Query Frame = 1

Query: 138 DLIRDLE---VKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPV--LLQEFVNHGGV 197
           DL ++L    +  P I KP  + G A +H M++V+    FK+L TPV  ++QE+V+H   
Sbjct: 298 DLAQNLSRAGLSLPCIVKPQVACGVADAHSMAIVFRVEDFKNLNTPVPAIIQEYVDHSSR 357

Query: 198 MFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEA 257
           +FK YV GE      +KS+P      +  E+     + F  +      S   N       
Sbjct: 358 IFKFYVLGETIFHAVKKSIPSSSSLRKSAEENGLKPILFDSLKSLPVDSANQN------P 417

Query: 258 AAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFD-MIRDRNGTYYVIDINYLPGFAVLPD- 317
            +E ++E+     VTE +  LR+ + + +F FD +I++  G + ++D+NYLP F  +PD 
Sbjct: 418 VSEIDLEL-----VTEAATWLRKKLDLTIFGFDVVIQEGTGDHVIVDLNYLPSFKEVPDN 477

Query: 318 ------YEAFLTKFFNEVMQK 326
                 +EA   +F   V +K
Sbjct: 478 IAVPAFWEAIRNRFDQHVQEK 487

BLAST of CmaCh02G004540 vs. NCBI nr
Match: gi|659118740|ref|XP_008459279.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo])

HSP 1 Score: 546.2 bits (1406), Expect = 4.4e-152
Identity = 277/341 (81.23%), Postives = 309/341 (90.62%), Query Frame = 1

Query: 8   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIH 67
           +S+S SS+  RVGYAFPPNKERNVIRPSLIDYAK HGVDLVRIDLQTP+LHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKFHGVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 68  KMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 127
           K+YDDAW + LQDFASKNPDVVVVDRPD I +LYNRVSMLDVV+QVKVSDSGV+IEVPKQ
Sbjct: 62  KLYDDAWAENLQDFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSGVRIEVPKQ 121

Query: 128 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 187
           +++ +++  ID I +LE+KFPVIAKP+ES+GSAKSHEMSLV+NRRG K+L TPVLLQEFV
Sbjct: 122 ILVKKEEEAIDSIMNLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKELNTPVLLQEFV 181

Query: 188 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 247
           NHGGVMFK+YVAG+QSVCVKRKSLPDV  TEEELEKKT+GAMKFSQISRAEEKSEKCN  
Sbjct: 182 NHGGVMFKIYVAGDQSVCVKRKSLPDVEGTEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 248 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRNGTYYVIDINYLPGFAV 307
            KKE   E+EIEMPPEK+V EVSRGL+EAMGIRLFNFDMIRD NGTYYVIDINYLPGFAV
Sbjct: 242 AKKE---EKEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDGNGTYYVIDINYLPGFAV 301

Query: 308 LPDYEAFLTKFFNEVMQKKVVEEENSAMEDEKAKPRCC-CF 348
           LP+YE FLTKFF EV +KK V+E  SA+ED+KAKPRCC CF
Sbjct: 302 LPEYEPFLTKFFKEVTEKK-VDEVASAIEDDKAKPRCCSCF 338

BLAST of CmaCh02G004540 vs. NCBI nr
Match: gi|449460744|ref|XP_004148105.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus])

HSP 1 Score: 537.0 bits (1382), Expect = 2.7e-149
Identity = 276/344 (80.23%), Postives = 306/344 (88.95%), Query Frame = 1

Query: 8   SSSSSSSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIH 67
           +S+S SS+  RVGYAFPPNKERNVIRPSLIDYAKLH VDLVRIDLQTP+LHQGPFHCIIH
Sbjct: 2   ASNSPSSNRLRVGYAFPPNKERNVIRPSLIDYAKLHSVDLVRIDLQTPLLHQGPFHCIIH 61

Query: 68  KMYDDAWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQ 127
           K+YDDAW + LQ+FASKNPDVVVVDRPD I +LYNRVSMLDVV+QVKVSDS VKIEVPKQ
Sbjct: 62  KLYDDAWAENLQEFASKNPDVVVVDRPDLISKLYNRVSMLDVVSQVKVSDSDVKIEVPKQ 121

Query: 128 VVLNRKDGVIDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFV 187
           +++ ++D  ID I  LE+KFPVIAKP+ES+GSAKSHEMSLV+NRRG KDL  PVL+QEFV
Sbjct: 122 ILVKQEDEAIDSIMKLELKFPVIAKPMESDGSAKSHEMSLVFNRRGLKDLNKPVLVQEFV 181

Query: 188 NHGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCG 247
           NHGGVMFK+YVAG+QS+CVKRKSLPDV ETEEELEKKT+GAMKFSQISRAEEKSEKCN  
Sbjct: 182 NHGGVMFKIYVAGDQSMCVKRKSLPDVEETEEELEKKTEGAMKFSQISRAEEKSEKCNGE 241

Query: 248 GKKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR-NGTYYVIDINYLPGFA 307
            KKE   EEEIEMPPEK+V EVSRGL+EAMGIRLFNFDMIRDR NG YYVIDINYLPGFA
Sbjct: 242 AKKE--DEEEIEMPPEKIVREVSRGLKEAMGIRLFNFDMIRDRNNGRYYVIDINYLPGFA 301

Query: 308 VLPDYEAFLTKFFNEVMQKKV-VEEENSAMEDEKAKPRCC-CFH 349
           VLP+YE FLTKFF EV +KKV V+E  SA E++K  PRCC CFH
Sbjct: 302 VLPEYEPFLTKFFKEVREKKVDVDEVASATENDKQNPRCCSCFH 343

BLAST of CmaCh02G004540 vs. NCBI nr
Match: gi|449460700|ref|XP_004148083.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus])

HSP 1 Score: 333.2 bits (853), Expect = 5.8e-88
Identity = 182/322 (56.52%), Postives = 219/322 (68.01%), Query Frame = 1

Query: 13  SSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDD 72
           SS  FR+GYA    KER+ I PSL+DYAKLHG+D VRID   P+  Q PFHCIIHK+YD 
Sbjct: 2   SSPRFRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 73  AWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNR 132
           +WV  LQDF S+ PDVVVVD P+ I RL NR SML+VV +VKV     +IE PKQVV+N 
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 133 KDGV----IDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVN 192
            D V    ++   DL +KFP+IAKPLESNGSAKSH++ LV N  G K L  P++LQEFVN
Sbjct: 122 LDVVLKNGLNTFSDLGLKFPIIAKPLESNGSAKSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 193 HGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGG 252
           HGGV+FKVYV GE  VCV RKSLPD+     E  KK D    FSQIS +  + +      
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDI---GPEDVKKLDAVSSFSQISNSGAQGDD----- 241

Query: 253 KKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDRN--GTYYVIDINYLPGFA 312
                 E  +EMP  + V  V+ GLREA G+RLFNFD+IRD N    Y VIDINYLPG+A
Sbjct: 242 ------EGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSNDHNRYLVIDINYLPGYA 301

Query: 313 VLPDYEAFLTKFFNEVMQKKVV 329
            +P+YE FLTKFF +V+Q + V
Sbjct: 302 KMPNYEPFLTKFFLDVVQNRTV 309

BLAST of CmaCh02G004540 vs. NCBI nr
Match: gi|659118544|ref|XP_008459175.1| (PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo])

HSP 1 Score: 329.3 bits (843), Expect = 8.4e-87
Identity = 179/322 (55.59%), Postives = 219/322 (68.01%), Query Frame = 1

Query: 13  SSDGFRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDD 72
           SS   R+GYA    KER+ I PSL+DYAKLHG+D VRID   P+  Q PFHCIIHK+YD 
Sbjct: 2   SSPRIRIGYALSLKKERSFILPSLVDYAKLHGIDFVRIDPLLPLTDQTPFHCIIHKLYDP 61

Query: 73  AWVDTLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNR 132
           +WV  LQDF S+ PDVVVVD P+ I RL NR SML+VV +VKV     +IE PKQVV+N 
Sbjct: 62  SWVQQLQDFTSQFPDVVVVDPPELISRLLNRDSMLEVVKEVKVPQGDERIETPKQVVVND 121

Query: 133 KDGV----IDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVN 192
            D +    +++  DL +KFPVIAKPLESNGSA+SH++ LV N  G K L  P++LQEFVN
Sbjct: 122 LDVLMKNGLNMFSDLGLKFPVIAKPLESNGSARSHQLCLVSNDSGLKGLNAPIVLQEFVN 181

Query: 193 HGGVMFKVYVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGG 252
           HGGV+FKVYV GE  VCV RKSLPD+     E  KK D    FSQIS +  + +      
Sbjct: 182 HGGVVFKVYVVGECVVCVTRKSLPDIA---PEDVKKLDAVSSFSQISNSGAQGDD----- 241

Query: 253 KKEAAAEEEIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR--NGTYYVIDINYLPGFA 312
                 E  +EMP  + V  V+ GLREA G+RLFNFD+IRD   +  Y VIDINYLPG+A
Sbjct: 242 ------EGNVEMPSLEFVMHVAAGLREATGLRLFNFDLIRDSSDHNRYLVIDINYLPGYA 301

Query: 313 VLPDYEAFLTKFFNEVMQKKVV 329
            +P+YE FLTKFF +V+Q + V
Sbjct: 302 KMPNYEPFLTKFFLDVVQNRTV 309

BLAST of CmaCh02G004540 vs. NCBI nr
Match: gi|703100607|ref|XP_010096964.1| (Inositol-tetrakisphosphate 1-kinase 1 [Morus notabilis])

HSP 1 Score: 309.7 bits (792), Expect = 6.9e-81
Identity = 161/346 (46.53%), Postives = 228/346 (65.90%), Query Frame = 1

Query: 17  FRVGYAFPPNKERNVIRPSLIDYAKLHGVDLVRIDLQTPILHQGPFHCIIHKMYDDAWVD 76
           F VGYA  P K+++ I+ SL+  A+  G+DLVRI+ + P+  QGPF C++HK+Y D W  
Sbjct: 6   FGVGYALAPKKQQSFIQDSLVSLARSRGIDLVRIEREIPLADQGPFDCVLHKLYGDDWRR 65

Query: 77  TLQDFASKNPDVVVVDRPDRIERLYNRVSMLDVVNQVKVSDSGVKIEVPKQVVLNRKDGV 136
            L+DF +KNP+ V++D P+ IERL+NR+SML VV+++K+ D      +PKQ+V+  K+ +
Sbjct: 66  QLEDFRAKNPNAVIIDYPEAIERLHNRISMLQVVSELKIEDRSETFGIPKQIVIYDKETL 125

Query: 137 IDLIRDLEVKFPVIAKPLESNGSAKSHEMSLVYNRRGFKDLETPVLLQEFVNHGGVMFKV 196
            D      +KFPVIAKPL ++GSAKSH+M+L++N  G  +L+ P++LQEFVNHGGV+FKV
Sbjct: 126 FDRPAWEGLKFPVIAKPLVADGSAKSHKMALIFNHDGLSNLKPPIVLQEFVNHGGVIFKV 185

Query: 197 YVAGEQSVCVKRKSLPDVVETEEELEKKTDGAMKFSQISRAEEKSEKCNCGGKKEAAAEE 256
           YV GE   CVKRKSLPDV  +EEE     +G + FSQ+S         +   KK     +
Sbjct: 186 YVVGEYVKCVKRKSLPDV--SEEEKLGSLEGLLSFSQVSNLATNERTDDKYYKKMHL--D 245

Query: 257 EIEMPPEKVVTEVSRGLREAMGIRLFNFDMIRDR--NGTYYVIDINYLPGFAVLPDYEAF 316
           E EMPP+  +T+++RGLR AM + LFNFD+IRD      Y +IDINY PG+A +P YE  
Sbjct: 246 ETEMPPQSFLTDIARGLRRAMKLNLFNFDVIRDARFGNRYLIIDINYFPGYAKMPGYETV 305

Query: 317 LTKFFNEVMQKKVVEEENSAMEDEKAKPRCCCFHERESQASTFEND 361
           LT FF ++M+KK  E E    E + A  R C    R+  ++ + +D
Sbjct: 306 LTDFFCDIMEKKEREVEKGLEEKDGADSRGCDKEMRKLVSNAYCSD 347

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ITPK1_ARATH6.9e-7347.04Inositol-tetrakisphosphate 1-kinase 1 OS=Arabidopsis thaliana GN=ITPK1 PE=1 SV=1[more]
ITPK1_MAIZE5.1e-6844.15Inositol-tetrakisphosphate 1-kinase 1 OS=Zea mays GN=ITPK1 PE=2 SV=1[more]
ITPK5_ORYSJ1.6e-6644.27Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. japonica GN=ITPK5 P... [more]
ITPK5_ORYSI1.6e-6644.27Inositol-tetrakisphosphate 1-kinase 5 OS=Oryza sativa subsp. indica GN=ITPK5 PE=... [more]
ITPK4_ORYSJ2.9e-6345.20Inositol-tetrakisphosphate 1-kinase 4 OS=Oryza sativa subsp. japonica GN=ITPK4 P... [more]
Match NameE-valueIdentityDescription
A0A0A0LRI5_CUCSA1.8e-14980.23Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439740 PE=3 SV=1[more]
A0A0A0LTT9_CUCSA4.1e-8856.52Inositol-tetrakisphosphate 1-kinase OS=Cucumis sativus GN=Csa_2G439240 PE=3 SV=1[more]
W9R373_9ROSA4.8e-8146.53Inositol-tetrakisphosphate 1-kinase OS=Morus notabilis GN=L484_017781 PE=3 SV=1[more]
A0A067EMQ3_CITSI5.3e-8051.23Inositol-tetrakisphosphate 1-kinase OS=Citrus sinensis GN=CISIN_1g047754mg PE=3 ... [more]
V4VTN2_9ROSI9.0e-8051.23Inositol-tetrakisphosphate 1-kinase OS=Citrus clementina GN=CICLE_v10024568mg PE... [more]
Match NameE-valueIdentityDescription
AT5G16760.13.9e-7447.04 Inositol 1,3,4-trisphosphate 5/6-kinase family protein[more]
AT4G33770.13.4e-6239.43 Inositol 1,3,4-trisphosphate 5/6-kinase family protein[more]
AT4G08170.22.7e-5940.58 Inositol 1,3,4-trisphosphate 5/6-kinase family protein[more]
AT2G43980.12.2e-1629.85 inositol 1,3,4-trisphosphate 5/6-kinase 4[more]
Match NameE-valueIdentityDescription
gi|659118740|ref|XP_008459279.1|4.4e-15281.23PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo][more]
gi|449460744|ref|XP_004148105.1|2.7e-14980.23PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus][more]
gi|449460700|ref|XP_004148083.1|5.8e-8856.52PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis sativus][more]
gi|659118544|ref|XP_008459175.1|8.4e-8755.59PREDICTED: inositol-tetrakisphosphate 1-kinase 1-like [Cucumis melo][more]
gi|703100607|ref|XP_010096964.1|6.9e-8146.53Inositol-tetrakisphosphate 1-kinase 1 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR008656Inositol_tetrakis-P_1-kinase
IPR011761ATP-grasp
IPR013816ATP-grasp fold, subdomain 2
Vocabulary: Molecular Function
TermDefinition
GO:0052725inositol-1,3,4-trisphosphate 6-kinase activity
GO:0052726inositol-1,3,4-trisphosphate 5-kinase activity
GO:0000287magnesium ion binding
GO:0005524ATP binding
GO:0047325inositol tetrakisphosphate 1-kinase activity
GO:0046872metal ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0005622intracellular
Vocabulary: Biological Process
TermDefinition
GO:0032957inositol trisphosphate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032957 inositol trisphosphate metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005622 intracellular
molecular_function GO:0005524 ATP binding
molecular_function GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity
molecular_function GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
molecular_function GO:0047325 inositol tetrakisphosphate 1-kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G004540.1CmaCh02G004540.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008656Inositol-tetrakisphosphate 1-kinasePIRPIRSF038186ITPKcoord: 1..335
score: 1.3E
IPR008656Inositol-tetrakisphosphate 1-kinasePFAMPF05770Ins134_P3_kincoord: 14..320
score: 6.8E
IPR011761ATP-grasp foldPROFILEPS50975ATP_GRASPcoord: 114..328
score: 15
IPR013816ATP-grasp fold, subdomain 2GENE3DG3DSA:3.30.470.20coord: 97..304
score: 2.
NoneNo IPR availablePANTHERPTHR14217FAMILY NOT NAMEDcoord: 17..339
score: 1.1E
NoneNo IPR availablePANTHERPTHR14217:SF5INOSITOL-TETRAKISPHOSPHATE 1-KINASE 1coord: 17..339
score: 1.1E
NoneNo IPR availableunknownSSF56059Glutathione synthetase ATP-binding domain-likecoord: 241..311
score: 7.3E-15coord: 93..213
score: 7.3

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None