CmaCh02G001850 (gene) Cucurbita maxima (Rimu)

BLAST of CmaCh02G001850 vs. Swiss-Prot
Match: PTR33_ARATH (Protein NRT1/ PTR FAMILY 6.4 OS=Arabidopsis thaliana GN=NPF6.4 PE=1 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.7e-182
Identity = 339/534 (63.48%), Postives = 397/534 (74.34%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+  VA SAS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GV+LLT+ATTI  MRPP CD    R  HQCI 
Sbjct: 111 TAL-------------------------GVLLLTVATTISSMRPPICD-DFRRLHHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LY ALYTIALGGGGIKSNVSGF SDQFD +DPKEEK M+FFFNRFYF+IS+ 
Sbjct: 171 ANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVI LVYVQD VGRGWGYGIS  TMV+A IVL+CGT  YRFKKP+GSP T IWRV  
Sbjct: 231 SLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGF 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRY---NPWIVS 300
           LAWKKR   +P HP  LN Y N  VP+T    CLDKAAI    ++ +   +   +PWIVS
Sbjct: 291 LAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVS 350

Query: 301 TVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-----------KSWQ 360
           TVT+VEEVK+V+KL+PIW+T ILFW IYSQMT +   +A+ +  +            ++ 
Sbjct: 351 TVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFL 410

Query: 361 VL-----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFA 420
           +L     TS+NE++F+P +R+LT   QG+TSLQRIG+G+VFS+  M  A  +E  RR  A
Sbjct: 411 ILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREAA 470

Query: 421 VGQNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFV 480
           V  +  IS FWL+PQ+FLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLST+SMGFFV
Sbjct: 471 VNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTISMGFFV 530

Query: 481 SSLLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYK 516
           SSLLV++V+ VT+K WL+SNLNK  LNYFYWLL VLG LNFL F++ A KHQYK
Sbjct: 531 SSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of CmaCh02G001850 vs. Swiss-Prot
Match: PTR7_ARATH (Protein NRT1/ PTR FAMILY 6.3 OS=Arabidopsis thaliana GN=NPF6.3 PE=1 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 3.4e-116
Identity = 243/551 (44.10%), Postives = 331/551 (60.07%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           +GI +NLVTYL   +HL +A +A  VTNFLG   +L LLGGF+AD  LGR+LT+A  A+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A                          GV +LTL+T IPG+RPP C+ +++     C  
Sbjct: 109 QAT-------------------------GVSILTLSTIIPGLRPPRCNPTTSSH---CEQ 168

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A+G QL +LY ALY  ALG GG+K++VSGF SDQFD  +PKE   M +FFNRF+F I++ 
Sbjct: 169 ASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVG 228

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SL AV VLVYVQD VGR WGYGI    +V+A+ V + GT  YRFKK  GSP+T +  V++
Sbjct: 229 SLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIV 288

Query: 241 LAWKKRSLPHPVHPRFLNDYQNA-----------KVPYTYRFMCLDKAAILDEVAAINQN 300
            AW+ R L  P  P +L D  +            K+P+T +F  LDKAAI D+ A +  N
Sbjct: 289 AAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSN 348

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY----------------IH 360
            +N W +ST+T+VEEVK ++++LPIW+T ILFW +++Q+T                  I 
Sbjct: 349 VFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIP 408

Query: 361 SRASIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
             +  ++      + T++ +++ I   +KL     GL  LQRIG+G+ F    M  A  V
Sbjct: 409 PASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALV 468

Query: 421 EKERRGFAVGQNTLISV-----FWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMST 480
           E +R   A      +       + LIPQ+ +VG GEA  Y GQL+FF+RE P+ MK MST
Sbjct: 469 ELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMST 528

Query: 481 GLFLSTLSMGFFVSSLLVTIVENVTNK--QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 518
           GL LSTL++GFF SS+LVTIVE  T K   W+  +LNKG L  FYWL+AVL  LNFL FL
Sbjct: 529 GLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFL 571

BLAST of CmaCh02G001850 vs. Swiss-Prot
Match: PTR27_ARATH (Protein NRT1/ PTR FAMILY 6.2 OS=Arabidopsis thaliana GN=NPF6.2 PE=1 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.0e-112
Identity = 246/543 (45.30%), Postives = 338/543 (62.25%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGRF T+   ++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A+                         G   L +AT +P +RPP C          CI 
Sbjct: 106 QAL-------------------------GTGALAVATKLPELRPPTCHHGEA-----CIP 165

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A   Q+ +LY +LY IALG GG+KS++SGF SDQFD  DPKE+  M FFFNRF+F IS+ 
Sbjct: 166 ATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMG 225

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           +L AV VLVY+QD VGR W YGI   +M IA+++ +CGT  YR+KK +GSP+  I++V+ 
Sbjct: 226 TLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIA 285

Query: 241 LAWKKR--SLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAI---------LDEVAAINQN 300
            A++KR   LP  +   + ++ +  ++ +T +F  LDKAAI         LD VA     
Sbjct: 286 AAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAI---- 345

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRAS------------ 360
             NPW +S+VT+VEEVKM+++LLPIW+T I+FW  Y+QM  +   +AS            
Sbjct: 346 -PNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIP 405

Query: 361 ----IIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
                ++   +  +  ++ ++  +PF +K      G +SLQRI IG+V S  GM AA  V
Sbjct: 406 AGSLTVFFVAAILITLAVYDRAIMPFWKKWK-GKPGFSSLQRIAIGLVLSTAGMAAAALV 465

Query: 421 EKERRGFA--VGQNTL-ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 480
           E++R   A    Q TL ISVF L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MSTGL
Sbjct: 466 EQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGL 525

Query: 481 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 510
           FL+TLS+GFFVSS LV+IV+ VT+      WL  N+N G L+YFYWLL +L  +NF+ ++
Sbjct: 526 FLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYI 552

BLAST of CmaCh02G001850 vs. Swiss-Prot
Match: PTR1_ARATH (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.1e-95
Identity = 207/549 (37.70%), Postives = 314/549 (57.19%), Query Frame = 1

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGR+ T+AT   I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFI- 106

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLTL+ ++PG++P +C+  +          
Sbjct: 107 ------------------------YVSGMTLLTLSASVPGLKPGNCNADTCHP------- 166

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
           N  Q A+ + ALY IALG GGIK  VS F +DQFD ND  E+     FFN FYF+I++ +
Sbjct: 167 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 226

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           L A  VLV++Q  VG GWG+G+    MVIAV     G+ FYR ++P GSPLT I++V++ 
Sbjct: 227 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 286

Query: 242 AWKKRSLPHPVHPRFL--------NDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           A++K S+  P     L        N   + K+ +T      DKAA+  +  +I     NP
Sbjct: 287 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNP 346

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + +VT+VEE+K ++ LLP+W+TGI+F  +YSQM+                   I S +
Sbjct: 347 WRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSAS 406

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S    T + ++  IP +RK T N +G T LQR+GIG+V SIF M+ AG +E  
Sbjct: 407 LSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVV 466

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +S+FW IPQ+ L+G  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 467 RLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 526

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+T+++G ++S++LVT+V  +T K     W+  NLN+G+L+YF++LLA L  LNFL +L
Sbjct: 527 SLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. Swiss-Prot
Match: PTR5_ARATH (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.6e-89
Identity = 203/549 (36.98%), Postives = 306/549 (55.74%), Query Frame = 1

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGR+ T+A+   I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVI- 107

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLT++ ++PG+ P      S    H    A
Sbjct: 108 ------------------------YIAGMTLLTISASVPGLTPT----CSGETCH----A 167

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
             GQ A+ + ALY IALG GGIK  VS F +DQFD  D KE+++   FFN FYF I++ +
Sbjct: 168 TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGA 227

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           + A  VLV++Q  VG GWG G+    M IAV+    G+ FYR +KP GSPLT + +V++ 
Sbjct: 228 MIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVA 287

Query: 242 AWKKRSLPHPVHPRFLNDYQNA--------KVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           + +K  +  P     L + Q+A        K+ +T      DKAA+  E       + + 
Sbjct: 288 SCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS 347

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM                    I S +
Sbjct: 348 WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSAS 407

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S      + +KL +PF+RK T + +G T LQRIGIG+V SIF MV+AG +E  
Sbjct: 408 LSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVA 467

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +++FW +PQ+FLVG  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 468 RLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 527

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNK----QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+ ++ G ++S+ LVT+V  VT       W+  NLN G+L+YF+WLLA L  LNFL +L
Sbjct: 528 SLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. TrEMBL
Match: J7LEY9_CUCSA (Nitrate transporter OS=Cucumis sativus GN=NRT1.3 PE=2 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 5.8e-240
Identity = 447/567 (78.84%), Postives = 478/567 (84.30%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADAKLGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPK EKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKGEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTM +AVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAILDE A    N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYATTGPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           EVEEVKMV KL+PIWSTGILFW IYSQMT +   +AS +  +  S+++            
Sbjct: 351 EVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAF YVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNKQWL+SNLNKG+L+YFYWLLAVLGLLNF FF+LLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNFFFFILLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A EMEGPIS KA ET
Sbjct: 591 NNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CmaCh02G001850 vs. TrEMBL
Match: A0A0A0LQY5_CUCSA (Oligopeptide transporter OS=Cucumis sativus GN=Csa_2G416080 PE=4 SV=1)

HSP 1 Score: 838.2 bits (2164), Expect = 5.8e-240
Identity = 447/567 (78.84%), Postives = 478/567 (84.30%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADAKLGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPK EKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKGEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTM +AVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAILDE A    N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYATTGPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           EVEEVKMV KL+PIWSTGILFW IYSQMT +   +AS +  +  S+++            
Sbjct: 351 EVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAF YVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNKQWL+SNLNKG+L+YFYWLLAVLGLLNF FF+LLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNFFFFILLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A EMEGPIS KA ET
Sbjct: 591 NNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CmaCh02G001850 vs. TrEMBL
Match: W9SDQ8_9ROSA (Nitrate transporter 1.3 OS=Morus notabilis GN=L484_004834 PE=4 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 3.7e-202
Identity = 379/565 (67.08%), Postives = 444/565 (78.58%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ +LH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+LTV  SA+I
Sbjct: 64  MGISMNLVTYLVGNLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYLTVVLSATI 123

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLH-QCI 120
           TA+                         GVI+LTLATTI GMRPP CD  + R LH QCI
Sbjct: 124 TAI-------------------------GVIILTLATTISGMRPPPCD--NYRNLHHQCI 183

Query: 121 AANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 180
            ANG QLA+LYAALYTIALGGGGIKSNVSGF SDQFDV+DPKEEKAM+FFFNRFYF ISI
Sbjct: 184 EANGNQLALLYAALYTIALGGGGIKSNVSGFGSDQFDVSDPKEEKAMIFFFNRFYFCISI 243

Query: 181 RSLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVV 240
            SLFAVIVLVYVQD VGRGWGYGIS GTMV+AV+VL+ GT FYRFKKPRGSPLTVIWRV+
Sbjct: 244 GSLFAVIVLVYVQDNVGRGWGYGISAGTMVLAVLVLLSGTPFYRFKKPRGSPLTVIWRVI 303

Query: 241 LLAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTV 300
            LAWKKR+ P+P HP  LN Y  A+VP+T +F CLDKAAILD+ A   +N+ NPWIVSTV
Sbjct: 304 FLAWKKRNQPYPSHPSLLNKYHEARVPHTEKFKCLDKAAILDDYAGSGENKNNPWIVSTV 363

Query: 301 TEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRA--------SIIYGEKSWQ----- 360
           T++EEVKMVLKL+PIWST ILFW +YSQ++ +   +A        S +    S+      
Sbjct: 364 TQIEEVKMVLKLIPIWSTCILFWTVYSQISTFAIEQATFMKRNIGSFVVPSGSFSTFVFI 423

Query: 361 ---VLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVG 420
              + TSMNEK+F+P +RKLT N QGLTSLQRIGIG+V S+  MVAA  VEK+RR F++ 
Sbjct: 424 SILLFTSMNEKIFVPLARKLTHNPQGLTSLQRIGIGLVLSMVAMVAAAIVEKQRREFSIH 483

Query: 421 QNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 480
            + ++S FWL+PQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS
Sbjct: 484 HDAILSAFWLVPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 543

Query: 481 LLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSN 540
           LLV++V+ VTNK WL+SNLN+G L  FYWLLAVL LLNF  FL+ A +HQYK+QHY   +
Sbjct: 544 LLVSLVDKVTNKSWLRSNLNRGRLENFYWLLAVLVLLNFGVFLIFASRHQYKEQHYLGPD 601

Query: 541 DNDE-ELKSSSNMIAHEMEGPISMK 548
           D++E ELKSS+++ A ++   I+M+
Sbjct: 604 DSEEKELKSSNDVTAEKLGEKIAME 601

BLAST of CmaCh02G001850 vs. TrEMBL
Match: A0A0B0PJ98_GOSAR (Nitrate transporter 1.3-like protein OS=Gossypium arboreum GN=F383_09957 PE=4 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 8.5e-199
Identity = 375/564 (66.49%), Postives = 434/564 (76.95%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+S+AKSATIVTNF+GALNLLGLLGGFLADAKLGR+LTVA SASI
Sbjct: 52  MGISMNLVTYLVGDLHISAAKSATIVTNFMGALNLLGLLGGFLADAKLGRYLTVALSASI 111

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GVILLTLATTIP MRPP+CD    R+ H+CI 
Sbjct: 112 TAL-------------------------GVILLTLATTIPSMRPPYCD-DYRRKHHECIE 171

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LYAALYT ALGGGGIKSNVSGF SDQFDV DPKEEKAM+FFFNRFYF IS+ 
Sbjct: 172 ANGRQLALLYAALYTTALGGGGIKSNVSGFGSDQFDVADPKEEKAMIFFFNRFYFGISLG 231

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVY+QD VGRGWGYGIS  TMVIAV+VL+CGT +YRFKKP+GSPLTV+WRV+L
Sbjct: 232 SLFAVIVLVYIQDNVGRGWGYGISAVTMVIAVVVLICGTPWYRFKKPQGSPLTVVWRVLL 291

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LA KK++ P+P HP  LNDY N KVPYT RF  LDKAAILD+  A N  + NPW+VSTVT
Sbjct: 292 LALKKKNQPYPSHPSLLNDYDNRKVPYTPRFKFLDKAAILDDNCAANAEKNNPWLVSTVT 351

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE----------------KS 360
            VEEVKMV+KLLPIWST ILFW IYSQMT +   +A+I++ +                 S
Sbjct: 352 HVEEVKMVIKLLPIWSTCILFWTIYSQMTTFTVEQATIMHRKIGSFVIPAGSFSAFLIIS 411

Query: 361 WQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
             + TS+NEKLF+P +RK+T N QG+TSLQRIGIG+VFS+  MV A  +EKERR  AV +
Sbjct: 412 ILLFTSLNEKLFVPLARKITHNPQGITSLQRIGIGLVFSVAAMVGAAIIEKERREIAVEK 471

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
              IS FWL+ QFFLVGAGEAFAYVGQLEFFIREAP+RMKSMSTGLFLST+SMGFFVSSL
Sbjct: 472 EVKISAFWLLVQFFLVGAGEAFAYVGQLEFFIREAPDRMKSMSTGLFLSTISMGFFVSSL 531

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LV+IV++VTN+ WL++NLNKG LN FYWLLAVLG LNFL FL+ A +HQYK Q       
Sbjct: 532 LVSIVDHVTNRNWLRNNLNKGKLNNFYWLLAVLGFLNFLVFLIFASRHQYKMQLPIEPES 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKA 549
            ++ELK  +     +ME   S++A
Sbjct: 592 GEKELKGLNGETIQDMEKKASLQA 589

BLAST of CmaCh02G001850 vs. TrEMBL
Match: A0A067K3W1_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13740 PE=4 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.5e-198
Identity = 374/558 (67.03%), Postives = 428/558 (76.70%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+ SAKSATIVTNF+G LNLLGLLGGFLADAKLGR+LTVA  ASI
Sbjct: 52  MGISMNLVTYLVGDLHIPSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYLTVAIFASI 111

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GV LLTLATTI  MRPP CD    RQ  QC+ 
Sbjct: 112 TAV-------------------------GVTLLTLATTISSMRPPPCDYR--RQHLQCVE 171

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LYAALYTIALGGGGIKSNVSGF SDQFD  DPKEEKAM+FFFNRFYF ISI 
Sbjct: 172 ANGQQLALLYAALYTIALGGGGIKSNVSGFGSDQFDTTDPKEEKAMIFFFNRFYFCISIG 231

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGIS GTMVIAV + +CGT +YRFKKP+GSP TVIWRV+ 
Sbjct: 232 SLFAVIVLVYVQDNVGRGWGYGISAGTMVIAVAIFLCGTRWYRFKKPQGSPFTVIWRVLF 291

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKR  P+P HP  LNDYQN+KVP+T RF CLDKAAI+D+ A+   N+ NPWIVS+V 
Sbjct: 292 LAWKKRGYPYPSHPSLLNDYQNSKVPHTERFKCLDKAAIMDDNASAGANKNNPWIVSSVM 351

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGEK----------------S 360
           EVE+VKMVLKL+PIWST ILFW +YSQMT +   +A+ +  +                 S
Sbjct: 352 EVEDVKMVLKLIPIWSTCILFWTVYSQMTTFTIEQATFMNRKVGSLIIPSGSLSTFLFIS 411

Query: 361 WQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
             + TS+NE+LF+P +R LT N+QGLTSLQRIGIG++FSI  MVAA  +EKERR  AV  
Sbjct: 412 ILLFTSLNERLFVPLARNLTHNLQGLTSLQRIGIGLIFSIVAMVAAAIIEKERREIAVEH 471

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           NT +S FWL+PQFFLVGAGEAFAYVGQLEFFIREAPE+MKSMSTG FLSTLS+GFF SSL
Sbjct: 472 NTKMSAFWLVPQFFLVGAGEAFAYVGQLEFFIREAPEKMKSMSTGFFLSTLSVGFFFSSL 531

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHY-SSSN 540
           LV+IV+  T K WLKSNLN+G LN FYWLLAVLG+LNF  FL +A KHQYK Q Y ++ N
Sbjct: 532 LVSIVDKATRKNWLKSNLNQGKLNNFYWLLAVLGVLNFFVFLAIAMKHQYKVQQYITNGN 582

Query: 541 DNDEELKSSSNMIAHEME 542
             ++E+K S+++I  EME
Sbjct: 592 YGEKEVKPSNDLIILEME 582

BLAST of CmaCh02G001850 vs. TAIR10
Match: AT3G21670.1 (AT3G21670.1 Major facilitator superfamily protein)

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 339/534 (63.48%), Postives = 397/534 (74.34%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+  VA SAS+
Sbjct: 51  MGISMNLVTYLVGDLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYKMVAISASV 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GV+LLT+ATTI  MRPP CD    R  HQCI 
Sbjct: 111 TAL-------------------------GVLLLTVATTISSMRPPICD-DFRRLHHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LY ALYTIALGGGGIKSNVSGF SDQFD +DPKEEK M+FFFNRFYF+IS+ 
Sbjct: 171 ANGHQLALLYVALYTIALGGGGIKSNVSGFGSDQFDTSDPKEEKQMIFFFNRFYFSISVG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVI LVYVQD VGRGWGYGIS  TMV+A IVL+CGT  YRFKKP+GSP T IWRV  
Sbjct: 231 SLFAVIALVYVQDNVGRGWGYGISAATMVVAAIVLLCGTKRYRFKKPKGSPFTTIWRVGF 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRY---NPWIVS 300
           LAWKKR   +P HP  LN Y N  VP+T    CLDKAAI    ++ +   +   +PWIVS
Sbjct: 291 LAWKKRKESYPAHPSLLNGYDNTTVPHTEMLKCLDKAAISKNESSPSSKDFEEKDPWIVS 350

Query: 301 TVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-----------KSWQ 360
           TVT+VEEVK+V+KL+PIW+T ILFW IYSQMT +   +A+ +  +            ++ 
Sbjct: 351 TVTQVEEVKLVMKLVPIWATNILFWTIYSQMTTFTVEQATFMDRKLGSFTVPAGSYSAFL 410

Query: 361 VL-----TSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFA 420
           +L     TS+NE++F+P +R+LT   QG+TSLQRIG+G+VFS+  M  A  +E  RR  A
Sbjct: 411 ILTILLFTSLNERVFVPLTRRLTKKPQGITSLQRIGVGLVFSMAAMAVAAVIENARREAA 470

Query: 421 VGQNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFV 480
           V  +  IS FWL+PQ+FLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLST+SMGFFV
Sbjct: 471 VNNDKKISAFWLVPQYFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTISMGFFV 530

Query: 481 SSLLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYK 516
           SSLLV++V+ VT+K WL+SNLNK  LNYFYWLL VLG LNFL F++ A KHQYK
Sbjct: 531 SSLLVSLVDRVTDKSWLRSNLNKARLNYFYWLLVVLGALNFLIFIVFAMKHQYK 558

BLAST of CmaCh02G001850 vs. TAIR10
Match: AT1G12110.1 (AT1G12110.1 nitrate transporter 1.1)

HSP 1 Score: 420.2 bits (1079), Expect = 1.9e-117
Identity = 243/551 (44.10%), Postives = 331/551 (60.07%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           +GI +NLVTYL   +HL +A +A  VTNFLG   +L LLGGF+AD  LGR+LT+A  A+I
Sbjct: 49  LGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAI 108

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A                          GV +LTL+T IPG+RPP C+ +++     C  
Sbjct: 109 QAT-------------------------GVSILTLSTIIPGLRPPRCNPTTSSH---CEQ 168

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A+G QL +LY ALY  ALG GG+K++VSGF SDQFD  +PKE   M +FFNRF+F I++ 
Sbjct: 169 ASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVG 228

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SL AV VLVYVQD VGR WGYGI    +V+A+ V + GT  YRFKK  GSP+T +  V++
Sbjct: 229 SLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIV 288

Query: 241 LAWKKRSLPHPVHPRFLNDYQNA-----------KVPYTYRFMCLDKAAILDEVAAINQN 300
            AW+ R L  P  P +L D  +            K+P+T +F  LDKAAI D+ A +  N
Sbjct: 289 AAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSN 348

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY----------------IH 360
            +N W +ST+T+VEEVK ++++LPIW+T ILFW +++Q+T                  I 
Sbjct: 349 VFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIP 408

Query: 361 SRASIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
             +  ++      + T++ +++ I   +KL     GL  LQRIG+G+ F    M  A  V
Sbjct: 409 PASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALV 468

Query: 421 EKERRGFAVGQNTLISV-----FWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMST 480
           E +R   A      +       + LIPQ+ +VG GEA  Y GQL+FF+RE P+ MK MST
Sbjct: 469 ELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMST 528

Query: 481 GLFLSTLSMGFFVSSLLVTIVENVTNK--QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 518
           GL LSTL++GFF SS+LVTIVE  T K   W+  +LNKG L  FYWL+AVL  LNFL FL
Sbjct: 529 GLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFL 571

BLAST of CmaCh02G001850 vs. TAIR10
Match: AT2G26690.1 (AT2G26690.1 Major facilitator superfamily protein)

HSP 1 Score: 408.7 bits (1049), Expect = 5.8e-114
Identity = 246/543 (45.30%), Postives = 338/543 (62.25%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGI++NLVTYL+  +HL S+ SA IVT+F+G   LL LLGGFLAD+ LGRF T+   ++I
Sbjct: 46  MGIAVNLVTYLMETMHLPSSTSANIVTDFMGTSFLLCLLGGFLADSFLGRFKTIGIFSTI 105

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
            A+                         G   L +AT +P +RPP C          CI 
Sbjct: 106 QAL-------------------------GTGALAVATKLPELRPPTCHHGEA-----CIP 165

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           A   Q+ +LY +LY IALG GG+KS++SGF SDQFD  DPKE+  M FFFNRF+F IS+ 
Sbjct: 166 ATAFQMTILYVSLYLIALGTGGLKSSISGFGSDQFDDKDPKEKAHMAFFFNRFFFFISMG 225

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           +L AV VLVY+QD VGR W YGI   +M IA+++ +CGT  YR+KK +GSP+  I++V+ 
Sbjct: 226 TLLAVTVLVYMQDEVGRSWAYGICTVSMAIAIVIFLCGTKRYRYKKSQGSPVVQIFQVIA 285

Query: 241 LAWKKR--SLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAI---------LDEVAAINQN 300
            A++KR   LP  +   + ++ +  ++ +T +F  LDKAAI         LD VA     
Sbjct: 286 AAFRKRKMELPQSIVYLYEDNPEGIRIEHTDQFHLLDKAAIVAEGDFEQTLDGVAI---- 345

Query: 301 RYNPWIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRAS------------ 360
             NPW +S+VT+VEEVKM+++LLPIW+T I+FW  Y+QM  +   +AS            
Sbjct: 346 -PNPWKLSSVTKVEEVKMMVRLLPIWATTIIFWTTYAQMITFSVEQASTMRRNIGSFKIP 405

Query: 361 ----IIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFV 420
                ++   +  +  ++ ++  +PF +K      G +SLQRI IG+V S  GM AA  V
Sbjct: 406 AGSLTVFFVAAILITLAVYDRAIMPFWKKWK-GKPGFSSLQRIAIGLVLSTAGMAAAALV 465

Query: 421 EKERRGFA--VGQNTL-ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 480
           E++R   A    Q TL ISVF L+PQFFLVGAGEAF Y GQL+FFI ++P+ MK+MSTGL
Sbjct: 466 EQKRLSVAKSSSQKTLPISVFLLVPQFFLVGAGEAFIYTGQLDFFITQSPKGMKTMSTGL 525

Query: 481 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 510
           FL+TLS+GFFVSS LV+IV+ VT+      WL  N+N G L+YFYWLL +L  +NF+ ++
Sbjct: 526 FLTTLSLGFFVSSFLVSIVKRVTSTSTDVGWLADNINHGRLDYFYWLLVILSGINFVVYI 552

BLAST of CmaCh02G001850 vs. TAIR10
Match: AT3G54140.1 (AT3G54140.1 peptide transporter 1)

HSP 1 Score: 352.1 bits (902), Expect = 6.4e-97
Identity = 207/549 (37.70%), Postives = 314/549 (57.19%), Query Frame = 1

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+  NLV YL   L+  +A +A  VTN+ G   +  L+G F+ADA LGR+ T+AT   I 
Sbjct: 47  GMGTNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFI- 106

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLTL+ ++PG++P +C+  +          
Sbjct: 107 ------------------------YVSGMTLLTLSASVPGLKPGNCNADTCHP------- 166

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
           N  Q A+ + ALY IALG GGIK  VS F +DQFD ND  E+     FFN FYF+I++ +
Sbjct: 167 NSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGA 226

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           L A  VLV++Q  VG GWG+G+    MVIAV     G+ FYR ++P GSPLT I++V++ 
Sbjct: 227 LIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVA 286

Query: 242 AWKKRSLPHPVHPRFL--------NDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           A++K S+  P     L        N   + K+ +T      DKAA+  +  +I     NP
Sbjct: 287 AFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAVESQSDSIKDGEVNP 346

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + +VT+VEE+K ++ LLP+W+TGI+F  +YSQM+                   I S +
Sbjct: 347 WRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTMDQHMGKNFEIPSAS 406

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S    T + ++  IP +RK T N +G T LQR+GIG+V SIF M+ AG +E  
Sbjct: 407 LSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVVSIFAMITAGVLEVV 466

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +S+FW IPQ+ L+G  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 467 RLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 526

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNKQ----WLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+T+++G ++S++LVT+V  +T K     W+  NLN+G+L+YF++LLA L  LNFL +L
Sbjct: 527 SLTTVALGNYLSTVLVTVVMKITKKNGKPGWIPDNLNRGHLDYFFYLLATLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. TAIR10
Match: AT5G01180.1 (AT5G01180.1 peptide transporter 5)

HSP 1 Score: 331.6 bits (849), Expect = 9.0e-91
Identity = 203/549 (36.98%), Postives = 306/549 (55.74%), Query Frame = 1

Query: 2   GISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASIT 61
           G+S NL+ YL   +++ +  ++  V+N+ G      L+G F+ADA LGR+ T+A+   I 
Sbjct: 48  GMSTNLINYLEKQMNMENVSASKSVSNWSGTCYATPLIGAFIADAYLGRYWTIASFVVI- 107

Query: 62  AVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIAA 121
                                   ++ G+ LLT++ ++PG+ P      S    H    A
Sbjct: 108 ------------------------YIAGMTLLTISASVPGLTPT----CSGETCH----A 167

Query: 122 NGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIRS 181
             GQ A+ + ALY IALG GGIK  VS F +DQFD  D KE+++   FFN FYF I++ +
Sbjct: 168 TAGQTAITFIALYLIALGTGGIKPCVSSFGADQFDDTDEKEKESKSSFFNWFYFVINVGA 227

Query: 182 LFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVLL 241
           + A  VLV++Q  VG GWG G+    M IAV+    G+ FYR +KP GSPLT + +V++ 
Sbjct: 228 MIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFFAGSNFYRLQKPGGSPLTRMLQVIVA 287

Query: 242 AWKKRSLPHPVHPRFLNDYQNA--------KVPYTYRFMCLDKAAILDEVAAINQNRYNP 301
           + +K  +  P     L + Q+A        K+ +T      DKAA+  E       + + 
Sbjct: 288 SCRKSKVKIPEDESLLYENQDAESSIIGSRKLEHTKILTFFDKAAVETESDNKGAAKSSS 347

Query: 302 WIVSTVTEVEEVKMVLKLLPIWSTGILFWAIYSQMTDY-----------------IHSRA 361
           W + TVT+VEE+K +++LLPIW+TGI+F ++YSQM                    I S +
Sbjct: 348 WKLCTVTQVEELKALIRLLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSAS 407

Query: 362 SIIYGEKSWQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKE 421
             ++   S      + +KL +PF+RK T + +G T LQRIGIG+V SIF MV+AG +E  
Sbjct: 408 LSLFDTLSVLFWAPVYDKLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVA 467

Query: 422 RRGFAVGQNTL------ISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGL 481
           R  +    N        +++FW +PQ+FLVG  E F ++GQLEFF  +AP+ M+S+ + L
Sbjct: 468 RLNYVQTHNLYNEETIPMTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSAL 527

Query: 482 FLSTLSMGFFVSSLLVTIVENVTNK----QWLKSNLNKGNLNYFYWLLAVLGLLNFLFFL 516
            L+ ++ G ++S+ LVT+V  VT       W+  NLN G+L+YF+WLLA L  LNFL +L
Sbjct: 528 SLTAIAFGNYLSTFLVTLVTKVTRSGGRPGWIAKNLNNGHLDYFFWLLAGLSFLNFLVYL 563

BLAST of CmaCh02G001850 vs. NCBI nr
Match: gi|659111835|ref|XP_008455932.1| (PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis melo])

HSP 1 Score: 844.3 bits (2180), Expect = 1.2e-241
Identity = 449/567 (79.19%), Postives = 483/567 (85.19%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADA+LGR+LTVAT ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADARLGRYLTVATFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPKEEKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKEEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTMVIAVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMVIAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAI+DE A  + N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAIIDEYATTDPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           E+EEVKMV KL+PIWSTGILFW IYSQMT +   +AS +     S+++            
Sbjct: 351 EIEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERRVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAFAYVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFAYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNK+WL+SNLNKG+L+YFYWLLAVLGLLNF FFLLLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKRWLRSNLNKGHLSYFYWLLAVLGLLNFFFFLLLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A E+EGPISMKA ET
Sbjct: 591 NNEELKISNDMVALEIEGPISMKAAET 589

BLAST of CmaCh02G001850 vs. NCBI nr
Match: gi|402810584|gb|AFR11353.1| (nitrate transporter [Cucumis sativus])

HSP 1 Score: 838.2 bits (2164), Expect = 8.4e-240
Identity = 447/567 (78.84%), Postives = 478/567 (84.30%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADAKLGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPK EKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKGEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTM +AVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAILDE A    N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYATTGPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           EVEEVKMV KL+PIWSTGILFW IYSQMT +   +AS +  +  S+++            
Sbjct: 351 EVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAF YVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNKQWL+SNLNKG+L+YFYWLLAVLGLLNF FF+LLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNFFFFILLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A EMEGPIS KA ET
Sbjct: 591 NNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CmaCh02G001850 vs. NCBI nr
Match: gi|778673402|ref|XP_011649985.1| (PREDICTED: protein NRT1/ PTR FAMILY 6.4 [Cucumis sativus])

HSP 1 Score: 838.2 bits (2164), Expect = 8.4e-240
Identity = 447/567 (78.84%), Postives = 478/567 (84.30%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLHL+SAKSATIVTNFLGALNLLGLLGGFLADAKLGR+LTVA  ASI
Sbjct: 51  MGISMNLVTYLVGDLHLTSAKSATIVTNFLGALNLLGLLGGFLADAKLGRYLTVAIFASI 110

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TAV                         GVILLTLATTIPGMRPPHCD  STRQLHQCI 
Sbjct: 111 TAV-------------------------GVILLTLATTIPGMRPPHCD-DSTRQLHQCIE 170

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANGGQLAMLYAALYTIALGGGGIKSNVSGF SDQFD NDPK EKAM+FFFNRFYFAISI 
Sbjct: 171 ANGGQLAMLYAALYTIALGGGGIKSNVSGFGSDQFDTNDPKGEKAMIFFFNRFYFAISIG 230

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVYVQD VGRGWGYGISGGTM +AVIVL+CGTTFYRFKKPRGSPLTVIWRVVL
Sbjct: 231 SLFAVIVLVYVQDKVGRGWGYGISGGTMAVAVIVLLCGTTFYRFKKPRGSPLTVIWRVVL 290

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LAWKKRSLPHP HP FLNDYQNAKVPYTYRF CLDKAAILDE A    N  NPWIVSTVT
Sbjct: 291 LAWKKRSLPHPAHPSFLNDYQNAKVPYTYRFKCLDKAAILDEYATTGPN--NPWIVSTVT 350

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE-KSWQV------------ 360
           EVEEVKMV KL+PIWSTGILFW IYSQMT +   +AS +  +  S+++            
Sbjct: 351 EVEEVKMVFKLIPIWSTGILFWTIYSQMTTFTVEQASFMERKVGSFEIPPGSMSAFLFIA 410

Query: 361 ---LTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
              +TS+NEKLFIP +RKLT NVQGLTSLQRIGIG+VFSIFGMVAAG VEKER+  AVGQ
Sbjct: 411 ILLVTSLNEKLFIPIARKLTHNVQGLTSLQRIGIGLVFSIFGMVAAGAVEKERKASAVGQ 470

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
           +T IS FWLIPQFFLVGAGEAF YVGQLEFFIREAPE+MKSMSTGLFLSTLSMGFFVSSL
Sbjct: 471 STRISAFWLIPQFFLVGAGEAFTYVGQLEFFIREAPEQMKSMSTGLFLSTLSMGFFVSSL 530

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LVTIV+ VTNKQWL+SNLNKG+L+YFYWLLAVLGLLNF FF+LLA+KHQYKDQHY SS D
Sbjct: 531 LVTIVDKVTNKQWLRSNLNKGHLSYFYWLLAVLGLLNFFFFILLARKHQYKDQHYVSSKD 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKATET 552
           N+EELK S++M+A EMEGPIS KA ET
Sbjct: 591 NNEELKISNDMVALEMEGPISKKAAET 589

BLAST of CmaCh02G001850 vs. NCBI nr
Match: gi|703153262|ref|XP_010110635.1| (Nitrate transporter 1.3 [Morus notabilis])

HSP 1 Score: 712.6 bits (1838), Expect = 5.3e-202
Identity = 379/565 (67.08%), Postives = 444/565 (78.58%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ +LH+SSAKSATIVTNF+G LNLLGLLGGFLADAKLGR+LTV  SA+I
Sbjct: 64  MGISMNLVTYLVGNLHISSAKSATIVTNFMGTLNLLGLLGGFLADAKLGRYLTVVLSATI 123

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLH-QCI 120
           TA+                         GVI+LTLATTI GMRPP CD  + R LH QCI
Sbjct: 124 TAI-------------------------GVIILTLATTISGMRPPPCD--NYRNLHHQCI 183

Query: 121 AANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISI 180
            ANG QLA+LYAALYTIALGGGGIKSNVSGF SDQFDV+DPKEEKAM+FFFNRFYF ISI
Sbjct: 184 EANGNQLALLYAALYTIALGGGGIKSNVSGFGSDQFDVSDPKEEKAMIFFFNRFYFCISI 243

Query: 181 RSLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVV 240
            SLFAVIVLVYVQD VGRGWGYGIS GTMV+AV+VL+ GT FYRFKKPRGSPLTVIWRV+
Sbjct: 244 GSLFAVIVLVYVQDNVGRGWGYGISAGTMVLAVLVLLSGTPFYRFKKPRGSPLTVIWRVI 303

Query: 241 LLAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTV 300
            LAWKKR+ P+P HP  LN Y  A+VP+T +F CLDKAAILD+ A   +N+ NPWIVSTV
Sbjct: 304 FLAWKKRNQPYPSHPSLLNKYHEARVPHTEKFKCLDKAAILDDYAGSGENKNNPWIVSTV 363

Query: 301 TEVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRA--------SIIYGEKSWQ----- 360
           T++EEVKMVLKL+PIWST ILFW +YSQ++ +   +A        S +    S+      
Sbjct: 364 TQIEEVKMVLKLIPIWSTCILFWTVYSQISTFAIEQATFMKRNIGSFVVPSGSFSTFVFI 423

Query: 361 ---VLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVG 420
              + TSMNEK+F+P +RKLT N QGLTSLQRIGIG+V S+  MVAA  VEK+RR F++ 
Sbjct: 424 SILLFTSMNEKIFVPLARKLTHNPQGLTSLQRIGIGLVLSMVAMVAAAIVEKQRREFSIH 483

Query: 421 QNTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 480
            + ++S FWL+PQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS
Sbjct: 484 HDAILSAFWLVPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSS 543

Query: 481 LLVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSN 540
           LLV++V+ VTNK WL+SNLN+G L  FYWLLAVL LLNF  FL+ A +HQYK+QHY   +
Sbjct: 544 LLVSLVDKVTNKSWLRSNLNRGRLENFYWLLAVLVLLNFGVFLIFASRHQYKEQHYLGPD 601

Query: 541 DNDE-ELKSSSNMIAHEMEGPISMK 548
           D++E ELKSS+++ A ++   I+M+
Sbjct: 604 DSEEKELKSSNDVTAEKLGEKIAME 601

BLAST of CmaCh02G001850 vs. NCBI nr
Match: gi|728845622|gb|KHG25065.1| (Nitrate transporter 1.3 -like protein [Gossypium arboreum])

HSP 1 Score: 701.4 bits (1809), Expect = 1.2e-198
Identity = 375/564 (66.49%), Postives = 434/564 (76.95%), Query Frame = 1

Query: 1   MGISMNLVTYLLWDLHLSSAKSATIVTNFLGALNLLGLLGGFLADAKLGRFLTVATSASI 60
           MGISMNLVTYL+ DLH+S+AKSATIVTNF+GALNLLGLLGGFLADAKLGR+LTVA SASI
Sbjct: 52  MGISMNLVTYLVGDLHISAAKSATIVTNFMGALNLLGLLGGFLADAKLGRYLTVALSASI 111

Query: 61  TAVVSSEPLRMASTEKFLLYPQLSRFLQGVILLTLATTIPGMRPPHCDISSTRQLHQCIA 120
           TA+                         GVILLTLATTIP MRPP+CD    R+ H+CI 
Sbjct: 112 TAL-------------------------GVILLTLATTIPSMRPPYCD-DYRRKHHECIE 171

Query: 121 ANGGQLAMLYAALYTIALGGGGIKSNVSGFRSDQFDVNDPKEEKAMLFFFNRFYFAISIR 180
           ANG QLA+LYAALYT ALGGGGIKSNVSGF SDQFDV DPKEEKAM+FFFNRFYF IS+ 
Sbjct: 172 ANGRQLALLYAALYTTALGGGGIKSNVSGFGSDQFDVADPKEEKAMIFFFNRFYFGISLG 231

Query: 181 SLFAVIVLVYVQDYVGRGWGYGISGGTMVIAVIVLVCGTTFYRFKKPRGSPLTVIWRVVL 240
           SLFAVIVLVY+QD VGRGWGYGIS  TMVIAV+VL+CGT +YRFKKP+GSPLTV+WRV+L
Sbjct: 232 SLFAVIVLVYIQDNVGRGWGYGISAVTMVIAVVVLICGTPWYRFKKPQGSPLTVVWRVLL 291

Query: 241 LAWKKRSLPHPVHPRFLNDYQNAKVPYTYRFMCLDKAAILDEVAAINQNRYNPWIVSTVT 300
           LA KK++ P+P HP  LNDY N KVPYT RF  LDKAAILD+  A N  + NPW+VSTVT
Sbjct: 292 LALKKKNQPYPSHPSLLNDYDNRKVPYTPRFKFLDKAAILDDNCAANAEKNNPWLVSTVT 351

Query: 301 EVEEVKMVLKLLPIWSTGILFWAIYSQMTDYIHSRASIIYGE----------------KS 360
            VEEVKMV+KLLPIWST ILFW IYSQMT +   +A+I++ +                 S
Sbjct: 352 HVEEVKMVIKLLPIWSTCILFWTIYSQMTTFTVEQATIMHRKIGSFVIPAGSFSAFLIIS 411

Query: 361 WQVLTSMNEKLFIPFSRKLTLNVQGLTSLQRIGIGVVFSIFGMVAAGFVEKERRGFAVGQ 420
             + TS+NEKLF+P +RK+T N QG+TSLQRIGIG+VFS+  MV A  +EKERR  AV +
Sbjct: 412 ILLFTSLNEKLFVPLARKITHNPQGITSLQRIGIGLVFSVAAMVGAAIIEKERREIAVEK 471

Query: 421 NTLISVFWLIPQFFLVGAGEAFAYVGQLEFFIREAPERMKSMSTGLFLSTLSMGFFVSSL 480
              IS FWL+ QFFLVGAGEAFAYVGQLEFFIREAP+RMKSMSTGLFLST+SMGFFVSSL
Sbjct: 472 EVKISAFWLLVQFFLVGAGEAFAYVGQLEFFIREAPDRMKSMSTGLFLSTISMGFFVSSL 531

Query: 481 LVTIVENVTNKQWLKSNLNKGNLNYFYWLLAVLGLLNFLFFLLLAQKHQYKDQHYSSSND 540
           LV+IV++VTN+ WL++NLNKG LN FYWLLAVLG LNFL FL+ A +HQYK Q       
Sbjct: 532 LVSIVDHVTNRNWLRNNLNKGKLNNFYWLLAVLGFLNFLVFLIFASRHQYKMQLPIEPES 589

Query: 541 NDEELKSSSNMIAHEMEGPISMKA 549
            ++ELK  +     +ME   S++A
Sbjct: 592 GEKELKGLNGETIQDMEKKASLQA 589