CmaCh01G008450 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G008450
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionNAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic
LocationCma_Chr01 : 4645692 .. 4645981 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCGAACATGTACTTATTCTAAGTGCCTATTTCTTTTCCATCGGTATCTATGGATTGATCACGATCCGAAATATGGTTAGGGCCCTTATGTGTCTTGAGCTTATACTGAATGCAGTTAATATGTATGAATTTTGTAACATTTTCTTGATAGTCGCCAAAGGGAATATTTTCTCAATTTTGGTTATAGCTATTGCACTAAAGCAGCTATTGGCCCAACTATTGTTTCGTCAATTTCTCGTAACAGAAAAGAAACTCATATCAATCAATCGAATTTGTTGAATAAGTAG

mRNA sequence

ATGCTCGAACATGTACTTATTCTAAGTGCCTATTTCTTTTCCATCGGTATCTATGGATTGATCACGATCCGAAATATGGTTAGGGCCCTTATGTGTCTTGAGCTTATACTGAATGCAGTTAATATTCGCCAAAGGGAATATTTTCTCAATTTTGGTTATAGCTATTGCACTAAAGCAGCTATTGGCCCAACTATTGTTTCGTCAATTTCTCGTAACAGAAAAGAAACTCATATCAATCAATCGAATTTGTTGAATAAGTAG

Coding sequence (CDS)

ATGCTCGAACATGTACTTATTCTAAGTGCCTATTTCTTTTCCATCGGTATCTATGGATTGATCACGATCCGAAATATGGTTAGGGCCCTTATGTGTCTTGAGCTTATACTGAATGCAGTTAATATTCGCCAAAGGGAATATTTTCTCAATTTTGGTTATAGCTATTGCACTAAAGCAGCTATTGGCCCAACTATTGTTTCGTCAATTTCTCGTAACAGAAAAGAAACTCATATCAATCAATCGAATTTGTTGAATAAGTAG

Protein sequence

MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQREYFLNFGYSYCTKAAIGPTIVSSISRNRKETHINQSNLLNK
BLAST of CmaCh01G008450 vs. Swiss-Prot
Match: NU4LC_ILLOL (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Illicium oligandrum GN=ndhE PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.9e-22
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR-------------QREY 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI              + + 
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDLFDSRQLKGDI 61

Query: 61  FLNFGYSY-CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
           F  F  +    +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSIYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. Swiss-Prot
Match: NU4LC_VITVI (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Vitis vinifera GN=ndhE PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 4.9e-22
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR-------------QREY 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI              + + 
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINFVTFSDFFDNRQLKGDI 61

Query: 61  FLNFGYSY-CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
           F  F  +    +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSIYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. Swiss-Prot
Match: NU4LC_MORIN (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Morus indica GN=ndhE PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 6.4e-22
Identity = 65/104 (62.50%), Postives = 69/104 (66.35%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQREYFLNFGYSY----- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI     F+ F   +     
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNIN----FVTFSDFFDSRQL 61

Query: 61  -------------CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                          +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  KGNIFSIFVIAIAAAEAAIGPAIVSSIYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. Swiss-Prot
Match: NU4LC_DRIGR (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Drimys granadensis GN=ndhE PE=3 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 6.4e-22
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR-------------QREY 60
           MLEHVLILSAY FSIGIYGLIT RNMVRALMCLELILNAVN+              + + 
Sbjct: 2   MLEHVLILSAYLFSIGIYGLITSRNMVRALMCLELILNAVNMNLIKFSDLFDSRQLKGDI 61

Query: 61  FLNFGYSY-CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
           F  F  +    +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSIHRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. Swiss-Prot
Match: NU4LC_CHLSC (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Chloranthus spicatus GN=ndhE PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 8.4e-22
Identity = 64/100 (64.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR-------------QREY 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVN+              + + 
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNMNLVTFSDLFDSRQLKGDI 61

Query: 61  FLNFGYSY-CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
           F  F  +    +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSIHRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TrEMBL
Match: A0A158N6I9_CERCN (NADH-plastoquinone oxidoreductase subunit 4L OS=Cercis canadensis GN=ndhE PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.1e-20
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR---------QREYFLNF 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI           R+   N 
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINFVTFSDFFDSRQLKGNI 61

Query: 61  GYSYC-----TKAAIGPTIVSSISRNRKETHINQSNLLNK 87
              +C      +AAIGP IVSS+ RNRK T INQSNLLNK
Sbjct: 62  FSIFCIAIAAAEAAIGPAIVSSVYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TrEMBL
Match: A0A142G678_ASTMO (NADH dehydrogenase subunit 4L OS=Astragalus mongholicus GN=ndhE PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TrEMBL
Match: A0A0K0LVC9_9FABA (NADH-quinone oxidoreductase subunit K OS=Oxytropis lambertii GN=ndhE PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TrEMBL
Match: A0A0U2L131_9FABA (NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Astragalus nakaianus GN=ndhE PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TrEMBL
Match: A0A0K0LUM9_9FABA (NADH-quinone oxidoreductase subunit K OS=Astragalus canadensis GN=ndhE PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 1.4e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. TAIR10
Match: ATCG01070.1 (ATCG01070.1 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L)

HSP 1 Score: 93.6 bits (231), Expect = 6.4e-20
Identity = 60/100 (60.00%), Postives = 68/100 (68.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVN---IRQREYFLNF---GYS 60
           +LEHVL+LSAY F IG+YGLIT RNMVRALMCLELILNAVN   +   ++F N    G  
Sbjct: 2   ILEHVLVLSAYLFLIGLYGLITSRNMVRALMCLELILNAVNMNFVTFSDFFDNSQLKGEI 61

Query: 61  YC--------TKAAIGPTIVSSISRNRKETHINQSNLLNK 87
           +C         +AAIG  IVSSI RNRK   INQS LLNK
Sbjct: 62  FCIFVIAIAAAEAAIGLAIVSSIYRNRKSIRINQSTLLNK 101

BLAST of CmaCh01G008450 vs. NCBI nr
Match: gi|584458793|gb|AHI95917.1| (NADH-plastoquinone oxidoreductase subunit 4L (chloroplast) [Cercis canadensis])

HSP 1 Score: 107.1 bits (266), Expect = 1.6e-20
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIR---------QREYFLNF 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI           R+   N 
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINFVTFSDFFDSRQLKGNI 61

Query: 61  GYSYC-----TKAAIGPTIVSSISRNRKETHINQSNLLNK 87
              +C      +AAIGP IVSS+ RNRK T INQSNLLNK
Sbjct: 62  FSIFCIAIAAAEAAIGPAIVSSVYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. NCBI nr
Match: gi|944543010|ref|YP_009175810.1| (NADH dehydrogenase subunit 4L (chloroplast) [Astragalus nakaianus])

HSP 1 Score: 106.7 bits (265), Expect = 2.1e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. NCBI nr
Match: gi|1002165462|ref|YP_009235593.1| (NdhE (chloroplast) [Cynanchum wilfordii])

HSP 1 Score: 106.3 bits (264), Expect = 2.7e-20
Identity = 65/104 (62.50%), Postives = 69/104 (66.35%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQREYFLNFGYSY----- 60
           MLEHVL+LSAY FS GIYGLIT RNMVRALMCLELILNAVNI     F+ F Y +     
Sbjct: 2   MLEHVLVLSAYLFSTGIYGLITSRNMVRALMCLELILNAVNIN----FVTFSYFFDNRQL 61

Query: 61  -------------CTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                          +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  RGDIFSIFVIAIAAAEAAIGPAIVSSIYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. NCBI nr
Match: gi|657302913|ref|YP_009041139.1| (NADH dehydrogenase subunit 4L [Rhazya stricta])

HSP 1 Score: 105.9 bits (263), Expect = 3.5e-20
Identity = 66/100 (66.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNI---RQREYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVNI   R  ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNINFVRFSDFFDNRQLKGEI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSI RNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSIYRNRKSTRINQSNLLNK 101

BLAST of CmaCh01G008450 vs. NCBI nr
Match: gi|748992348|gb|AJE72772.1| (NADH dehydrogenase subunit 4L (plastid) [Astragalus crassicarpus])

HSP 1 Score: 105.9 bits (263), Expect = 3.5e-20
Identity = 65/100 (65.00%), Postives = 70/100 (70.00%), Query Frame = 1

Query: 1   MLEHVLILSAYFFSIGIYGLITIRNMVRALMCLELILNAVNIRQ---REYFLNFGYS--- 60
           MLEHVL+LSAY FSIGIYGLIT RNMVRALMCLELILNAVN+      ++F N       
Sbjct: 2   MLEHVLVLSAYLFSIGIYGLITSRNMVRALMCLELILNAVNLNLVTFSDFFDNRQLKGNI 61

Query: 61  --------YCTKAAIGPTIVSSISRNRKETHINQSNLLNK 87
                      +AAIGP IVSSISRNRK T INQSNLLNK
Sbjct: 62  FSIFVIAIAAAEAAIGPAIVSSISRNRKSTRINQSNLLNK 101

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NU4LC_ILLOL4.9e-2265.00NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Illicium oligandrum ... [more]
NU4LC_VITVI4.9e-2265.00NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Vitis vinifera GN=nd... [more]
NU4LC_MORIN6.4e-2262.50NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Morus indica GN=ndhE... [more]
NU4LC_DRIGR6.4e-2265.00NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Drimys granadensis G... [more]
NU4LC_CHLSC8.4e-2264.00NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Chloranthus spicatus... [more]
Match NameE-valueIdentityDescription
A0A158N6I9_CERCN1.1e-2065.00NADH-plastoquinone oxidoreductase subunit 4L OS=Cercis canadensis GN=ndhE PE=3 S... [more]
A0A142G678_ASTMO1.4e-2066.00NADH dehydrogenase subunit 4L OS=Astragalus mongholicus GN=ndhE PE=3 SV=1[more]
A0A0K0LVC9_9FABA1.4e-2066.00NADH-quinone oxidoreductase subunit K OS=Oxytropis lambertii GN=ndhE PE=3 SV=1[more]
A0A0U2L131_9FABA1.4e-2066.00NAD(P)H-quinone oxidoreductase subunit 4L, chloroplastic OS=Astragalus nakaianus... [more]
A0A0K0LUM9_9FABA1.4e-2066.00NADH-quinone oxidoreductase subunit K OS=Astragalus canadensis GN=ndhE PE=3 SV=1[more]
Match NameE-valueIdentityDescription
ATCG01070.16.4e-2060.00ATCG01070.1 NADH-ubiquinone/plastoquinone oxidoreductase chain 4L[more]
Match NameE-valueIdentityDescription
gi|584458793|gb|AHI95917.1|1.6e-2065.00NADH-plastoquinone oxidoreductase subunit 4L (chloroplast) [Cercis canadensis][more]
gi|944543010|ref|YP_009175810.1|2.1e-2066.00NADH dehydrogenase subunit 4L (chloroplast) [Astragalus nakaianus][more]
gi|1002165462|ref|YP_009235593.1|2.7e-2062.50NdhE (chloroplast) [Cynanchum wilfordii][more]
gi|657302913|ref|YP_009041139.1|3.5e-2066.00NADH dehydrogenase subunit 4L [Rhazya stricta][more]
gi|748992348|gb|AJE72772.1|3.5e-2065.00NADH dehydrogenase subunit 4L (plastid) [Astragalus crassicarpus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001133NADH_UbQ_OxRdtase_chain4L/K
Vocabulary: Molecular Function
TermDefinition
GO:0016651oxidoreductase activity, acting on NAD(P)H
Vocabulary: Biological Process
TermDefinition
GO:0042773ATP synthesis coupled electron transport
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042773 ATP synthesis coupled electron transport
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0016020 membrane
cellular_component GO:0009536 plastid
molecular_function GO:0016651 oxidoreductase activity, acting on NAD(P)H

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G008450.1CmaCh01G008450.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001133NADH-ubiquinone oxidoreductase chain 4L/KPANTHERPTHR11434NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT ND4Lcoord: 1..86
score: 3.0
IPR001133NADH-ubiquinone oxidoreductase chain 4L/KPFAMPF00420Oxidored_q2coord: 5..42
score: 8.7
NoneNo IPR availablePANTHERPTHR11434:SF6NAD(P)H-QUINONE OXIDOREDUCTASE SUBUNIT 4L, CHLOROPLASTICcoord: 1..86
score: 3.0

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None