CmaCh01G004020 (gene) Cucurbita maxima (Rimu) | Cucurbit Genomics Database (CuGenDB)

Overview


Name	CmaCh01G004020
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu))
Description	Delta(7)-sterol-C5(6)-desaturase 1-like protein
Location	Cma_Chr01 : 2038533 .. 2041442 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCCTCTGGTTTTTCCACTCGCCGGAGTTGGGCTGATTCCGACGGCCATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTTATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGGTCTTTGCCTTTATTGTTCTTATATCTCAACTGCCTCATTAATGAGTTCTAATTCTAGTCATTTGGGTTCTCTTATTTCATTTTGTTCTTCGTTTCAGCAATGAATTCTGTCATTGGAGAAAATCTGGATATGTATTTCTTTTAATTTCAGTCACTGTTCTTCAGATTCATGGACCTGAATCCTTCAACTCTGGATATTGCATGCTATATTTGATTCATTTTGTGCTACTGCAAGGCTTTGATTCAGATCAAATTTACATATCTTTGATGGGGCTGTTTTTTTCCCTTCTGAGATGCTAGTGGTTCTGGTCTTTTCCATGATTACGATTTATTTGTGCAAGTTTCTGTTCTAAGAAGAACTGTAATAACCATTACAACTGCTACTTAAAGAGGGTAGTTTAGCTCTACAAATGGATGATTCTGCTACTCATGATGAACATATATGTCTAACCAAAAGTTATGTGTCACAGCTCAATGCTTGTATGATTGTTTGACCTGTTGGACACTTGATTGCAGATTCTATTCCATCAAATAAATTGATGCTCTTGCAGATTCATGCAACAATCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATATGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTTTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGTAACTTCTTAACTTACTATCTCAATTTTTGCAACATTTAATTCATGTCAAATAAACTTCTTTCATGTCATTCTTGTTTGATTTCCTTAAGATGTGTTAAACGAAGCCCAAATACAAACCGATCTCGATCGAGAACTATTTCCCAAAAGATCTCTTACAAAAAAACTAACCCATCCTAACAGAAGGAAAATATAAGCTTAGCATTAAGTTGCTGCTGTTAACTGTTAGGACAGTAGTAGGTATTGAATCTCGAACTCTTGTGTAACCGCCCAAGCTTATCGCTAGTAGATAGTCTATTTTGGGCTTTTCAAAACGCGTCTGCTAGGGAGAAGTTTCCACTATCCTTATAAGGAATGCTTCGTTCCCCTCTCCAACCAATGTGGGATCTCACCATCCACCTCCCCTGGGGCCCAACGTCTTTGCTAACACACGACCCGGTAACTTTCTCACCATCCACCTCCCTTGGGGGCTAGCATCCTCACTAGCACACCACCCGGTAACTTTCTCACCATCCACCTCCCTTGGGGGCTAGCATCCTCACCAGCACACCACCCGGTAACTTTCTTTGATACCATTTGTAACGGCCCAAGCCTATCACTAGCAGATATTGTCTTTGGGTTTTTCCTTCTAGGCTTCGGTTTTAAAACGTGTTTTCTAGGGAGAGGTTTCCACACCCTTGTAAGGAATGTTTCGTTCCCCTCTCCGACCAATGTGAGATCTCACATTTTGTTTCCTTAAAACCATATGAGTTTTTGAATAATGCCAATTGAAAACGATATCGATCTATCGTGAACATTAGGAGTGGCTACTCGCCTCCATAGGGCTTACTTGAGTCACACGAACTTGACTTTTGATGTGCTCTTCTTATATCATTGTGGCATTTATCTTATCCGAAAACTCTTCCTATATCGACTAAATAGAGCTCGTTTTAGCGACAAAGACTGCAAGTTTTATATCAAATGCATTGATTCATTTTGACAGAAACTTCAGAATCTGTTCAGCTTGAGTTGATTAGAGAAATATAGATATTGTTCTCTTAGAATGAAAAAGTTCTTTGGTTGTTTCTCATTAATGGAAAATTATGTGATACATGGTCTGTTTGTATGACAGGACTAGCATTTCACCCGGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACGCTTCTTGACCCAATGGATACACAACACAAGTTGTCATGATCGGTTGCTTCAACACATTATAGCTTTTGTTGATTCTTTGTGTTCAATGTAAATCAAGTTGGTCATGTTTGTACAAGTCACGAGACGACTCTCTGACTTCACAACATTGTCAAATTTAACATCTTACGAATTACCTTGACAGCAAAATGGAGTAAAGCCAGACAGTTGTCTCATACAATTAGTCAAGATGATAGCTTCATTGAACGAGTGTTTCAGGCGTTGTACCTGTTGCAAGAGAGAAATGAAGCACTACCAAAAGCAAGTTGGAATGAGAGACATTGACAACTTGCTTTTTCTTTTACTTTTTTTTTCCCATTTCTATATGCATCTCCTTACTTTTCAAAGCAATAGGCAATGTCCAAAACAGTCTAGAGCTCCCATTGTTCATCCTTCAATAATAAAAGGTATTCAT

mRNA sequence

TTCCTCTGGTTTTTCCACTCGCCGGAGTTGGGCTGATTCCGACGGCCATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTTATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGATTCTATTCCATCAAATAAATTGATGCTCTTGCAGATTCATGCAACAATCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATATGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTTTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGACTAGCATTTCACCCGGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACGCTTCTTGACCCAATGGATACACAACACAAGTTGTCATGATCGGTTGCTTCAACACATTATAGCTTTTGTTGATTCTTTGTGTTCAATGTAAATCAAGTTGGTCATGTTTGTACAAGTCACGAGACGACTCTCTGACTTCACAACATTGTCAAATTTAACATCTTACGAATTACCTTGACAGCAAAATGGAGTAAAGCCAGACAGTTGTCTCATACAATTAGTCAAGATGATAGCTTCATTGAACGAGTGTTTCAGGCGTTGTACCTGTTGCAAGAGAGAAATGAAGCACTACCAAAAGCAAGTTGGAATGAGAGACATTGACAACTTGCTTTTTCTTTTACTTTTTTTTTCCCATTTCTATATGCATCTCCTTACTTTTCAAAGCAATAGGCAATGTCCAAAACAGTCTAGAGCTCCCATTGTTCATCCTTCAATAATAAAAGGTATTCAT

Coding sequence (CDS)

ATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTTATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGATTCTATTCCATCAAATAAATTGATGCTCTTGCAGATTCATGCAACAATCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATATGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTTTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGACTAGCATTTCACCCGGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACGCTTCTTGACCCAATGGATACACAACACAAGTTGTCATGA

Protein sequence

MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS

Homology

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BLAST of CmaCh01G004020 vs. Swiss-Prot
Match: SC5D_TOBAC (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 4.3e-112
Identity = 178/266 (66.92%), Postives = 216/266 (81.20%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL+ F+EETS YNR+VLGT  P+    P PH+LQ WLRN IGG L+YF SGFLWCF+IY+
Sbjct: 4   YLKQFVEETSFYNRLVLGTFMPESWWGPLPHMLQGWLRNYIGGVLLYFISGFLWCFYIYH 63

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRNVY+PKD+IPSNK MLLQI   +KAMP YC LP+ SEY++E+ WT+C+ R+ DVGWL
Sbjct: 64  LKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISDVGWL 123

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
           +Y  +   YL  VEF IYWMH  LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 124 SYVIYAAIYLVIVEFGIYWMHMELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ AL L+P+HF THI L+F E +WT NIHDCIH +++PVMGAGYHT+HH  YR
Sbjct: 184 ILQAVPHVVALLLVPMHFSTHIALIFLEALWTANIHDCIHGKVFPVMGAGYHTIHHRTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDWMFGTL DP++   K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEEDAK 269

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BLAST of CmaCh01G004020 vs. Swiss-Prot
Match: SC5D1_ARATH (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2)

HSP 1 Score: 386.7 bits (992), Expect = 2.1e-106
Identity = 170/261 (65.13%), Postives = 208/261 (79.69%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  F++ETS YNRIVL  + P  + +P PH LQTWLRN + G L+YF SGFLWCF+IYY
Sbjct: 7   YLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVY+PKD+IP+ K M LQ+   +KAMP Y +LPT SE ++E  WT+C+  + + GW+
Sbjct: 67  LKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWI 126

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHPVDG
Sbjct: 127 LYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDG 186

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALF++PIHF THI LLF E +WT NIHDCIH  +WPVMGAGYHT+HHT Y+
Sbjct: 187 ILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYK 246

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L DP+
Sbjct: 247 HNYGHYTIWMDWMFGSLRDPL 267

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BLAST of CmaCh01G004020 vs. Swiss-Prot
Match: SC5D2_ARATH (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.7e-100
Identity = 160/261 (61.30%), Postives = 202/261 (77.39%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           Y +  + ETS YNR+VL  + P  + +P PH LQTWLRN + G ++YF SGFLWCF+IYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVYVPK+SIP+ K MLLQI+  +KAMP Y +LP  SEY++EH WT+CY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
               ++  YL  VEF IYW+H+ LHD+K LYK+LHA HHMYNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQAIPH+ ALF++PIH  TH+ LLF E +WT +IHDCIH  +WP+MGAGYHT+HHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

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BLAST of CmaCh01G004020 vs. Swiss-Prot
Match: SC5D_RAT (Lathosterol oxidase OS=Rattus norvegicus GN=Sc5d PE=1 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 1.1e-22
Identity = 74/233 (31.76%), Postives = 124/233 (53.22%), Query Frame = 1


Query: 47  IGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSE 106
           +G +++YFF   L  +F+Y    +  +       N++   +I  T+K++P +  +PT S 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVSR-EIMFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTRCYPRVFDV--GWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAK 166
           +++E   +++ Y  + D   GW+     V ++L F +  IYW+HR LH  + LYK++H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHH-RLLYKHIHKP 159

Query: 167 HHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHD 226
           HH++      +PFA  AFHPVDG LQ++P+    F+ P+H   ++ L     VWT +IHD
Sbjct: 160 HHIWKIP---TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 ---CIHAQMWP-VMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
               +     P + G+ +HT HH  + YNYG Y    D + G+   P   + K
Sbjct: 220 GDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGK 263

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BLAST of CmaCh01G004020 vs. Swiss-Prot
Match: SC5D_MOUSE (Lathosterol oxidase OS=Mus musculus GN=Sc5d PE=2 SV=2)

HSP 1 Score: 105.9 bits (263), Expect = 7.1e-22
Identity = 73/233 (31.33%), Postives = 124/233 (53.22%), Query Frame = 1


Query: 47  IGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSE 106
           +G +++YFF   L  +F+Y    +  +       N++   +I  T+K++P +  +PT S 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVSR-EIVFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTRCYPRVFDV--GWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAK 166
           +++E   +++ Y  + D   GW+     V ++L F +  IYW+HR LH  + +YK +H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLIYWIHRGLHH-RLVYKRIHKP 159

Query: 167 HHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHD 226
           HH++      +PFA  AFHPVDG LQ++P+    F+ P+H   ++ L     VWT +IHD
Sbjct: 160 HHIWKIP---TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 ---CIHAQMWP-VMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
               +   + P + G+ +HT HH  + YNYG Y    D + G+   P   + K
Sbjct: 220 GDFRVPQILRPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKHPSSFEGK 263

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BLAST of CmaCh01G004020 vs. TrEMBL
Match: A0A0A0L3B9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 3.8e-147
Identity = 239/272 (87.87%), Postives = 255/272 (93.75%), Query Frame = 1


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG PPYLELF+EETS YNRIVLGTIFP+F LDPFPHILQTWLRNC+GGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSNK MLLQI  TIKAMPLYCVLPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFH+ TYLTF+EF IYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEVVWT NIHD IH++MWPVMGAGYHT+
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HHTRYRYNYGHYSIWMDWMFGTLLDPMD + K
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAK 272

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BLAST of CmaCh01G004020 vs. TrEMBL
Match: M1AP00_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400010415 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 5.5e-114
Identity = 185/266 (69.55%), Postives = 219/266 (82.33%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL LF+EETS +NRIVLGT  P+    P PH  Q WLRN IGG L+YF SGFLWCF+IYY
Sbjct: 4   YLNLFVEETSFFNRIVLGTFLPESWWGPLPHWFQGWLRNYIGGVLLYFLSGFLWCFYIYY 63

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRNVY+PKD+IPSNK MLLQI   +KAMP YC LP+ SEY++E+ WT+C+ R+ DVGW+
Sbjct: 64  LKRNVYLPKDAIPSNKAMLLQIGVAMKAMPWYCALPSLSEYMIENGWTKCFSRISDVGWM 123

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  +V  YL  VEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 124 PYLIYVAVYLLIVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALFL+P+HF THI LLF E +WT NIHDCIHA++WPVMGAGYHT+HHT YR
Sbjct: 184 ILQAVPHVVALFLLPVHFTTHIALLFIEAIWTANIHDCIHAKVWPVMGAGYHTIHHTTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDWMFGTL DP++ + K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEDEVK 269

[Close]

BLAST of CmaCh01G004020 vs. TrEMBL
Match: K4B6K9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 7.2e-114
Identity = 184/266 (69.17%), Postives = 219/266 (82.33%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL LF+EETS +NRIVLGT  P+    P PH  Q WLRN IGG L+YF SGFLWCF+IYY
Sbjct: 4   YLNLFVEETSFFNRIVLGTFLPESWWGPLPHWFQGWLRNYIGGVLLYFISGFLWCFYIYY 63

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRNVY+PKD+IPSN+ MLLQI   +KAMP YC LP+ SEY++E+ WT+C+ R+ DVGW+
Sbjct: 64  LKRNVYLPKDAIPSNRAMLLQIGVAMKAMPWYCALPSLSEYMIENGWTKCFSRISDVGWI 123

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  +V  YL  VEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 124 PYLIYVAVYLVIVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALFL+P+HF THI LLF E +WT NIHDCIHA++WPVMGAGYHT+HHT YR
Sbjct: 184 ILQAVPHVIALFLLPVHFTTHIALLFIEAIWTANIHDCIHAKVWPVMGAGYHTIHHTTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDWMFGTL DP++ + K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEDEVK 269

[Close]

BLAST of CmaCh01G004020 vs. TrEMBL
Match: A0A059AE11_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J01509 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 9.3e-114
Identity = 186/263 (70.72%), Postives = 217/263 (82.51%), Query Frame = 1


Query: 10  LFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYYWKR 69
           LF EETSLYNRIVLG++ P    +P P +LQTWLRN IGG LIYF SGFLWCF+IYY KR
Sbjct: 110 LFTEETSLYNRIVLGSLLPASAWEPMPRLLQTWLRNYIGGTLIYFLSGFLWCFYIYYLKR 169

Query: 70  NVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWLAYA 129
           NVYVPKD IP+ K MLLQI+  +KAMP YC LPT SEY+VE+ WT+C+ R+ DVGWLAY 
Sbjct: 170 NVYVPKDEIPTRKAMLLQIYVAMKAMPWYCALPTLSEYMVENGWTKCFSRISDVGWLAYL 229

Query: 130 FHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDGILQ 189
            +++ YL   EF IYWMHR LHD+KPLYK+LHA HH+YNK+ TLSPFAGLAFHP+DGILQ
Sbjct: 230 VYLSIYLVMAEFGIYWMHRELHDIKPLYKHLHATHHIYNKQNTLSPFAGLAFHPLDGILQ 289

Query: 190 AIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYRYNY 249
           A+PH+ ALFL+P HF THI LLF E +WT NIHDCIH ++WPVMGAGYHT+HHT YR+NY
Sbjct: 290 AVPHVMALFLVPTHFTTHIALLFLEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYRHNY 349

Query: 250 GHYSIWMDWMFGTLLDPMDTQHK 273
           GHY+IWMDWMFGTL DP+D   K
Sbjct: 350 GHYTIWMDWMFGTLQDPIDDGSK 372

[Close]

BLAST of CmaCh01G004020 vs. TrEMBL
Match: I1N6T9_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=1)

HSP 1 Score: 416.0 bits (1068), Expect = 3.5e-113
Identity = 182/266 (68.42%), Postives = 219/266 (82.33%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           Y  LF+E+T LYNRIVLGT+ P FV  P PH LQTWLRN +GG L+Y  SG LWCF+IYY
Sbjct: 16  YSRLFVEDTDLYNRIVLGTLLPHFVWGPLPHFLQTWLRNYLGGVLLYLLSGLLWCFYIYY 75

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
           WKRNVYVPKD+IPS + MLLQI   +KAMP Y +LPT SEY+VE  WT+CYPR+++VGWL
Sbjct: 76  WKRNVYVPKDAIPSRRAMLLQISVAMKAMPWYTLLPTVSEYLVETGWTKCYPRLYNVGWL 135

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
           AY  ++  YL+ VEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 136 AYLVYLAIYLSIVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 195

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH   LF +PIHF TH++L+F E +WT NIHDCIH ++WPVMGAGYHT+HHT YR
Sbjct: 196 ILQALPHSLCLFFMPIHFTTHLVLIFIEGIWTANIHDCIHGKLWPVMGAGYHTIHHTTYR 255

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDW+FGTL +P + + K
Sbjct: 256 HNYGHYTIWMDWLFGTLHNPEEDEGK 281

[Close]

BLAST of CmaCh01G004020 vs. TAIR10
Match: AT3G02580.1 (AT3G02580.1 sterol 1)

HSP 1 Score: 386.7 bits (992), Expect = 1.2e-107
Identity = 170/261 (65.13%), Postives = 208/261 (79.69%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  F++ETS YNRIVL  + P  + +P PH LQTWLRN + G L+YF SGFLWCF+IYY
Sbjct: 7   YLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVY+PKD+IP+ K M LQ+   +KAMP Y +LPT SE ++E  WT+C+  + + GW+
Sbjct: 67  LKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWI 126

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHPVDG
Sbjct: 127 LYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDG 186

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALF++PIHF THI LLF E +WT NIHDCIH  +WPVMGAGYHT+HHT Y+
Sbjct: 187 ILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYK 246

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L DP+
Sbjct: 247 HNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of CmaCh01G004020 vs. TAIR10
Match: AT3G02590.1 (AT3G02590.1 Fatty acid hydroxylase superfamily protein)

HSP 1 Score: 367.1 bits (941), Expect = 9.6e-102
Identity = 160/261 (61.30%), Postives = 202/261 (77.39%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           Y +  + ETS YNR+VL  + P  + +P PH LQTWLRN + G ++YF SGFLWCF+IYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVYVPK+SIP+ K MLLQI+  +KAMP Y +LP  SEY++EH WT+CY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
               ++  YL  VEF IYW+H+ LHD+K LYK+LHA HHMYNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQAIPH+ ALF++PIH  TH+ LLF E +WT +IHDCIH  +WP+MGAGYHT+HHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of CmaCh01G004020 vs. TAIR10
Match: AT1G02205.3 (AT1G02205.3 Fatty acid hydroxylase superfamily)

HSP 1 Score: 49.3 bits (116), Expect = 4.4e-06
Identity = 41/151 (27.15%), Postives = 72/151 (47.68%), Query Frame = 1


Query: 139 VEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG-----ILQAIPH 198
           VEF  YW+H+ALH     + +L++++H ++  + ++       HP        IL AIP 
Sbjct: 138 VEFLYYWLHKALH-----HHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFILFAIPL 197

Query: 199 L------------FALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLH 258
           L            FA ++I I F  ++    FE++     H       +      YH+LH
Sbjct: 198 LTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH-LFPPLKFLCYTPSYHSLH 257

Query: 259 HTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HT++R NY  +    D+++GT+ +  DT ++
Sbjct: 258 HTQFRTNYSLFMPLYDYIYGTMDESTDTLYE 282

[Close]

BLAST of CmaCh01G004020 vs. NCBI nr
Match: gi|449465599|ref|XP_004150515.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus])

HSP 1 Score: 528.9 bits (1361), Expect = 5.4e-147
Identity = 239/272 (87.87%), Postives = 255/272 (93.75%), Query Frame = 1


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG PPYLELF+EETS YNRIVLGTIFP+F LDPFPHILQTWLRNC+GGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSNK MLLQI  TIKAMPLYCVLPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFH+ TYLTF+EF IYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEVVWT NIHD IH++MWPVMGAGYHT+
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HHTRYRYNYGHYSIWMDWMFGTLLDPMD + K
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAK 272

[Close]

BLAST of CmaCh01G004020 vs. NCBI nr
Match: gi|659086753|ref|XP_008444093.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo])

HSP 1 Score: 525.8 bits (1353), Expect = 4.6e-146
Identity = 238/272 (87.50%), Postives = 254/272 (93.38%), Query Frame = 1


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG PPYLELF+EETS YNRIVLGTIFP+F L PFPHILQTWLRNC+GGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLYPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSNK MLLQI  TIKAMPLYCVLPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFH+ TYLTF+EF IYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEVVWT NIHD IH++MWPVMGAGYHT+
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HHTRYRYNYGHYSIWMDWMFGTLLDPMD + K
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAK 272

[Close]

BLAST of CmaCh01G004020 vs. NCBI nr
Match: gi|694435112|ref|XP_009344734.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Pyrus x bretschneideri])

HSP 1 Score: 419.1 bits (1076), Expect = 6.0e-114
Identity = 183/267 (68.54%), Postives = 220/267 (82.40%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  FL+ET+ YNRIVLG++ P+ V DP PH  QTWLRN +GG L+Y  SGFLWC +IYY
Sbjct: 5   YLNPFLDETTFYNRIVLGSLVPNRVWDPLPHFFQTWLRNYVGGTLLYLVSGFLWCLYIYY 64

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRN Y+PKD++PSNK MLLQI+  +KAMP YC LPT SEY+VE+ W +C+ R+ DVGW 
Sbjct: 65  LKRNTYIPKDAVPSNKAMLLQIYVAMKAMPWYCALPTVSEYMVENGWAKCFARISDVGWP 124

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
           AY  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 125 AYLLYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPIDG 184

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALFL+P+HF THI LLF E +WT NIHDCIHA++WPVMGAGYHT+HHT YR
Sbjct: 185 ILQAVPHVIALFLVPMHFTTHIALLFIEAIWTANIHDCIHAKIWPVMGAGYHTIHHTTYR 244

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHKL 274
           +NYGHY+IWMDWM GTL DP+D + K+
Sbjct: 245 HNYGHYTIWMDWMCGTLRDPLDDELKV 271

[Close]

BLAST of CmaCh01G004020 vs. NCBI nr
Match: gi|565360293|ref|XP_006346904.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Solanum tuberosum])

HSP 1 Score: 418.7 bits (1075), Expect = 7.9e-114
Identity = 185/266 (69.55%), Postives = 219/266 (82.33%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL LF+EETS +NRIVLGT  P+    P PH  Q WLRN IGG L+YF SGFLWCF+IYY
Sbjct: 4   YLNLFVEETSFFNRIVLGTFLPESWWGPLPHWFQGWLRNYIGGVLLYFLSGFLWCFYIYY 63

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRNVY+PKD+IPSNK MLLQI   +KAMP YC LP+ SEY++E+ WT+C+ R+ DVGW+
Sbjct: 64  LKRNVYLPKDAIPSNKAMLLQIGVAMKAMPWYCALPSLSEYMIENGWTKCFSRISDVGWM 123

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  +V  YL  VEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 124 PYLIYVAVYLLIVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALFL+P+HF THI LLF E +WT NIHDCIHA++WPVMGAGYHT+HHT YR
Sbjct: 184 ILQAVPHVVALFLLPVHFTTHIALLFIEAIWTANIHDCIHAKVWPVMGAGYHTIHHTTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDWMFGTL DP++ + K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEDEVK 269

[Close]

BLAST of CmaCh01G004020 vs. NCBI nr
Match: gi|658007026|ref|XP_008338691.1| (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Malus domestica])

HSP 1 Score: 418.7 bits (1075), Expect = 7.9e-114
Identity = 182/267 (68.16%), Postives = 220/267 (82.40%), Query Frame = 1


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  FLEET+ YNRIVLG++ P+   DP PH  QTWLRN +GG L+Y  SGFLWC +IYY
Sbjct: 5   YLNPFLEETTFYNRIVLGSLVPNXXWDPLPHFFQTWLRNYVGGTLLYLVSGFLWCLYIYY 64

Query: 67  WKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRN+Y+PKD++PSNK MLLQI+  +KAMP YC LPT SEY+VE+ W +C+ R+ DVGW 
Sbjct: 65  LKRNIYIPKDAVPSNKAMLLQIYVAMKAMPWYCALPTVSEYMVENGWAKCFARISDVGWP 124

Query: 127 AYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
           AY  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 125 AYLSYLAIYLVFVEFGIYWMHRXLHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPIDG 184

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALFL+P+HF THI LLF E +WT NIHDCIHA++WPVMGAGYHT+HHT YR
Sbjct: 185 ILQAVPHVIALFLVPMHFTTHIALLFIEAIWTANIHDCIHAKIWPVMGAGYHTIHHTTYR 244

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHKL 274
           +NYGHY+IWMDWM GTL DP++ + K+
Sbjct: 245 HNYGHYTIWMDWMCGTLRDPLEDESKV 271

The following BLAST results are available for this feature:

BLAST of CmaCh01G004020 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SC5D_TOBAC	4.3e-112	66.92	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum PE=2 SV=1	[more]
SC5D1_ARATH	2.1e-106	65.13	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana GN=STE1 PE=1 SV=2	[more]
SC5D2_ARATH	1.7e-100	61.30	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana GN=HDF7 PE=3...	[more]
SC5D_RAT	1.1e-22	31.76	Lathosterol oxidase OS=Rattus norvegicus GN=Sc5d PE=1 SV=1	[more]
SC5D_MOUSE	7.1e-22	31.33	Lathosterol oxidase OS=Mus musculus GN=Sc5d PE=2 SV=2	[more]

BLAST of CmaCh01G004020 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3B9_CUCSA	3.8e-147	87.87	Uncharacterized protein OS=Cucumis sativus GN=Csa_4G409800 PE=3 SV=1	[more]
M1AP00_SOLTU	5.5e-114	69.55	Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400010415 PE=3 SV=1	[more]
K4B6K9_SOLLC	7.2e-114	69.17	Uncharacterized protein OS=Solanum lycopersicum PE=3 SV=1	[more]
A0A059AE11_EUCGR	9.3e-114	70.72	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J01509 PE=3 SV=1	[more]
I1N6T9_SOYBN	3.5e-113	68.42	Uncharacterized protein OS=Glycine max PE=3 SV=1	[more]

BLAST of CmaCh01G004020 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G02580.1	1.2e-107	65.13	sterol 1	[more]
AT3G02590.1	9.6e-102	61.30	Fatty acid hydroxylase superfamily protein	[more]
AT1G02205.3	4.4e-06	27.15	Fatty acid hydroxylase superfamily	[more]

BLAST of CmaCh01G004020 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449465599\|ref\|XP_004150515.1\|	5.4e-147	87.87	PREDICTED: delta(7)-sterol-C5(6)-desaturase [Cucumis sativus]	[more]
gi\|659086753\|ref\|XP_008444093.1\|	4.6e-146	87.50	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo]	[more]
gi\|694435112\|ref\|XP_009344734.1\|	6.0e-114	68.54	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Pyrus x bretschneideri]	[more]
gi\|565360293\|ref\|XP_006346904.1\|	7.9e-114	69.55	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Solanum tuberosum]	[more]
gi\|658007026\|ref\|XP_008338691.1\|	7.9e-114	68.16	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Malus domestica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR006694	Fatty_acid_hydroxylase

Vocabulary: **Molecular Function**
Term	Definition
GO:0005506	iron ion binding
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0006633	fatty acid biosynthetic process
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh01G004020.1	CmaCh01G004020.1	mRNA

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 134..262 score: 6.6
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 7..272 score: 3.5E
None	No IPR available	PANTHER	PTHR11863:SF33	LATHOSTEROL OXIDASE	coord: 7..272 score: 3.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
CmaCh01G004020	CmoCh14G012010	Cucurbita moschata (Rifu)	cmacmoB463
CmaCh01G004020	CmoCh01G004270	Cucurbita moschata (Rifu)	cmacmoB468
CmaCh01G004020	Cp4.1LG02g13820	Cucurbita pepo (Zucchini)	cmacpeB491
CmaCh01G004020	Cp4.1LG03g15170	Cucurbita pepo (Zucchini)	cmacpeB495
CmaCh01G004020	Carg07496	Silver-seed gourd	carcmaB1059

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
CmaCh01G004020	CmaCh14G011780	Cucurbita maxima (Rimu)	cmacmaB256

The following block(s) are covering this gene:


Gene	Organism	Block
CmaCh01G004020	Cucumber (Gy14) v2	cgybcmaB368
CmaCh01G004020	Melon (DHL92) v3.6.1	cmamedB496
CmaCh01G004020	Melon (DHL92) v3.6.1	cmamedB497
CmaCh01G004020	Silver-seed gourd	carcmaB0365
CmaCh01G004020	Silver-seed gourd	carcmaB0669
CmaCh01G004020	Cucumber (Chinese Long) v3	cmacucB0547
CmaCh01G004020	Watermelon (97103) v2	cmawmbB473
CmaCh01G004020	Wax gourd	cmawgoB0562
CmaCh01G004020	Cucurbita maxima (Rimu)	cmacmaB110
CmaCh01G004020	Cucurbita maxima (Rimu)	cmacmaB255
CmaCh01G004020	Cucumber (Gy14) v1	cgycmaB0011
CmaCh01G004020	Cucurbita moschata (Rifu)	cmacmoB447
CmaCh01G004020	Cucurbita moschata (Rifu)	cmacmoB462
CmaCh01G004020	Wild cucumber (PI 183967)	cmacpiB458
CmaCh01G004020	Wild cucumber (PI 183967)	cmacpiB485
CmaCh01G004020	Cucumber (Chinese Long) v2	cmacuB455
CmaCh01G004020	Cucumber (Chinese Long) v2	cmacuB482
CmaCh01G004020	Melon (DHL92) v3.5.1	cmameB384
CmaCh01G004020	Melon (DHL92) v3.5.1	cmameB431
CmaCh01G004020	Melon (DHL92) v3.5.1	cmameB435
CmaCh01G004020	Watermelon (Charleston Gray)	cmawcgB407
CmaCh01G004020	Watermelon (97103) v1	cmawmB437
CmaCh01G004020	Watermelon (97103) v1	cmawmB465
CmaCh01G004020	Cucurbita pepo (Zucchini)	cmacpeB476
CmaCh01G004020	Cucurbita pepo (Zucchini)	cmacpeB494
CmaCh01G004020	Bottle gourd (USVL1VR-Ls)	cmalsiB440