CmaCh01G003470 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G003470
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionUbiquitin-protein ligase/ zinc ion binding protein
LocationCma_Chr01 : 1723603 .. 1725899 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGGTTCTCGATCATCGGACGGCGAAAAGGAGAGGCGAAGTTCGGTCAACTCTAGCAGATGGAAGAACGAGACGATGAAAGTCATACTTACCGATCCTCAAGTTCTCGATTGCTATATTTGTTGCGTGCCCTTGTCCGTTCCTGTTTACCAGGTTCGATTAAGCTTTTTTTCCGCTTGCATTTTGCTTACGATTATCGTTCTTGTTAATCTTCTTGTCTATGATGCTTTGTTCTTCTCGTCCATTTTTTTTCTTGCAATTTTGACTACTGTAGTTCACTTAGTGTGTTTGCTGGATTTGACGGTTTCCAAATCCCCCTTACTATGAGATTTTAACCTTCTCGTTTAGAAGTTTATTGTGCAGACTGTGGATTTCCAATGGAAGTTATGGATTATGATAAAAATTAAAGAAATATGCCGAAGTTTTGAACAGTTATTGGCAAAGATCGTGGAGGTATGGATTGTAGAACTGAAGATATCAAATGTTTTGATGTAAATAATCTTTGAACATGAAAATTATTCTGGATATTATGGGGACATAATCACAAATGGTTGGGATTGAATTGAAATCTTTGGTGAGGATTGTTGGAGTCTACGTTGGCTATGAGGAAGGCTATGAGTATATAAGCAACGGATACTATCTTCATTGGTATGAAGCCTTTTGGAGAAACCAAAAGTAAAGCCATGAGAGCTTATCACATCATTGTGGCCCACATGTTCCTATCAAGTAGTATCATAGTCTTATTCTTATCTTAACTTAGCGGTTTCCATAGAATCCTCAGGTTTCGAATAAAAAAAGTTGTGAGCCTAGAAGGTGTGACTTAGATGTCGAACAAAAAAAAAAAGTGAGCCTCGAAGTTGTAGTCATAGTGAAGATCCGTGGTAGAGCTCTATTGAACTAGCAATGTGCTCTAGAAAAGAGGAGTTGTCTAAAATAGTGTTAAGACAGACACGTGACTATTAAAAAGAGACGCAATAGTGTACTCTGTTCGAAGGGAGGATTGTTGAGGATTGTTGAAGTCCCACATTGGCCAATTAAGATGGAAGACCGTGGGTATATAAATAAGGGACACTATCTTCTTTGGTATGAAGCGTTTAGGGAAATCAAAGGCCAAGCCATGAGAGATTATACTCATACTGGACAATATCATTCTAATGTGAAGGTCCCTTGTTCCTATCAATCTTGCCCTGAATTGCTTTTCTTGTTACATTATCTCGAGCAAATGCCAAAAATCAAGCTGTACTTATTTGATATTTTTTTCACCTTGAACTTGAAAGGGATGAATATAGAAGAAAATAAGCATATATTGGTGTATATAATCTCTTTTCTTGTTATCTTTGAGTCTATTTTATGTCATATAGCATATACTTTGGTGCTGAGATGTTGATTGTTGGATGAATTAGTGTGAGAATGGGCATATTTCTTGTTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCTAGCTGCAGCTTGCCAATTGGCTACATGCGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATTAAGATGCCCTGCCAAAACTCAAGATACGGATGCAAAACTACAACGAATTTGAACCAAATAAATGAGCATGAGACATTGTGCCCATATCAACCCTGTTCGTGCCCTTTATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCCGCCAAAAGCATCTCATACAATTCAAGGTTCACAATATGCTTGAACAGTGATGATAAATATTGTATCTTTCAAGCAGAGAACGATGGTGTTTTGTTTGTCCTCTCTTACTCGTTTGAAACTTTTGGTGATGCGGTTACTTTGAAAAGGATTGGACCTCGGTCGTCTGAGAAAAAGTTTTGTTATGATATTAAAGCCAAATCACACGGGAATGTTCTTTCTTTGCTATCAGTGGCGAAGGAGATCCAACGTTGGACTGAAGATCCTCCTTCCAAGGGATCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAGGACCAACATTGAGGATCATCACAGGTGCACATTCATTTTAAAACAAAGCTCAATTATTGTTTAAACTGTCTTAACGTTGTTGGCTGGCTCTGATCTTTGTCACCTAAACTTTCTATCTGGGGGGGAAGGACTTGGTGCATGCTTTTGTGCATATCATCATCTTTTATCATGCATATCCAGTAATAAAATGCATCTTTTGTGTATGATAGATGGATAGCATATTCAACATTGTC

mRNA sequence

ATGGCGGGTTCTCGATCATCGGACGGCGAAAAGGAGAGGCGAAGTTCGGTCAACTCTAGCAGATGGAAGAACGAGACGATGAAAGTCATACTTACCGATCCTCAAGTTCTCGATTGCTATATTTGTTGCGTGCCCTTGTCCGTTCCTGTTTACCAGTGTGAGAATGGGCATATTTCTTGTTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCTAGCTGCAGCTTGCCAATTGGCTACATGCGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATTAAGATGCCCTGCCAAAACTCAAGATACGGATGCAAAACTACAACGAATTTGAACCAAATAAATGAGCATGAGACATTGTGCCCATATCAACCCTGTTCGTGCCCTTTATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCCGCCAAAAGCATCTCATACAATTCAAGGTTCACAATATGCTTGAACAGTGATGATAAATATTGTATCTTTCAAGCAGAGAACGATGGTGTTTTGTTTGTCCTCTCTTACTCGTTTGAAACTTTTGGTGATGCGGTTACTTTGAAAAGGATTGGACCTCGGTCGTCTGAGAAAAAGTTTTGTTATGATATTAAAGCCAAATCACACGGGAATGTTCTTTCTTTGCTATCAGTGGCGAAGGAGATCCAACGTTGGACTGAAGATCCTCCTTCCAAGGGATCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAGGACCAACATTGAGGATCATCACAGGTGCACATTCATTTTAAAACAAAGCTCAATTATTGTTTAAACTGTCTTAACGTTGTTGGCTGGCTCTGATCTTTGTCACCTAAACTTTCTATCTGGGGGGGAAGGACTTGGTGCATGCTTTTGTGCATATCATCATCTTTTATCATGCATATCCAGTAATAAAATGCATCTTTTGTGTATGATAGATGGATAGCATATTCAACATTGTC

Coding sequence (CDS)

ATGGCGGGTTCTCGATCATCGGACGGCGAAAAGGAGAGGCGAAGTTCGGTCAACTCTAGCAGATGGAAGAACGAGACGATGAAAGTCATACTTACCGATCCTCAAGTTCTCGATTGCTATATTTGTTGCGTGCCCTTGTCCGTTCCTGTTTACCAGTGTGAGAATGGGCATATTTCTTGTTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCTAGCTGCAGCTTGCCAATTGGCTACATGCGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATTAAGATGCCCTGCCAAAACTCAAGATACGGATGCAAAACTACAACGAATTTGAACCAAATAAATGAGCATGAGACATTGTGCCCATATCAACCCTGTTCGTGCCCTTTATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCCGCCAAAAGCATCTCATACAATTCAAGGTTCACAATATGCTTGAACAGTGATGATAAATATTGTATCTTTCAAGCAGAGAACGATGGTGTTTTGTTTGTCCTCTCTTACTCGTTTGAAACTTTTGGTGATGCGGTTACTTTGAAAAGGATTGGACCTCGGTCGTCTGAGAAAAAGTTTTGTTATGATATTAAAGCCAAATCACACGGGAATGTTCTTTCTTTGCTATCAGTGGCGAAGGAGATCCAACGTTGGACTGAAGATCCTCCTTCCAAGGGATCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGA

Protein sequence

MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSKGSLLIPNEFFGSSSAQTILEIRIWPSR
BLAST of CmaCh01G003470 vs. Swiss-Prot
Match: SIL10_ARATH (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 6.4e-52
Identity = 108/276 (39.13%), Postives = 161/276 (58.33%), Query Frame = 1

Query: 3   GSRSSDGEKE-----RR-----SSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQ 62
           GS S D ++E     RR     +S +SS  K+  + V L DP VLDC ICC PL +P++Q
Sbjct: 67  GSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQ 126

Query: 63  CENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQ 122
           C+NGH++C+ CC+KV N+CPSC+LPIGY+RCRA+EKV+E+ ++ C N++YGCK +T+   
Sbjct: 127 CDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGN 186

Query: 123 INEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKY 182
              HE +C + PCSCP+ DC Y    + L +H R  H+D   S  +N+R TI L+ + K 
Sbjct: 187 RFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKT 246

Query: 183 CIFQAENDGVLFVLSYSFETFGDAVTLKRIGPRS-SEKKFCYDIKAKSHGNVLSLLSVAK 242
            I Q ENDG + V+          V++  I P +    +    +   +  ++L    + K
Sbjct: 247 TILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVK 306

Query: 243 EIQRWTEDPPSKGSLLIPNEFFGSSSAQTILEIRIW 268
            IQ+ T + P  G +LIP+  F   S    L ++IW
Sbjct: 307 NIQKVTNEHPEDGFMLIPSYLF---SGNDNLNLQIW 339

BLAST of CmaCh01G003470 vs. Swiss-Prot
Match: SINL7_ARATH (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-38
Identity = 88/268 (32.84%), Postives = 144/268 (53.73%), Query Frame = 1

Query: 3   GSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSS 62
           GS S   +K + SS ++++   +    +L D ++LDC IC    ++P++QC+NGH++CSS
Sbjct: 15  GSNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSS 74

Query: 63  CCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPY 122
           CC K++NKCP+C+ P+G+ RCRA+E VLESI +PC N++ GCK   +  +   HE  C +
Sbjct: 75  CCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMF 134

Query: 123 QPCSCPLSDCSYVDSAEHLGSHFRKRHQD--SAKSISYNSRFTICLNSDDKYCIFQAEND 182
             C+CP  DC+Y  S + L +H+R  H +     +   +   ++ +N   K  I      
Sbjct: 135 SHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLT 194

Query: 183 GVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKSHGNVLSLLS-VAKEIQRWTE 242
             LF +    E +G  VT+  I P S E  ++ Y +     G+ +   S   K + + + 
Sbjct: 195 NHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSF 254

Query: 243 DPPSKGSLLIPNEFFGSSSAQTILEIRI 267
             P +  +LIPN          +LE+RI
Sbjct: 255 QTPQENFMLIPNSLLRGD----VLEMRI 278

BLAST of CmaCh01G003470 vs. Swiss-Prot
Match: SIL11_ARATH (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 1.8e-38
Identity = 92/258 (35.66%), Postives = 135/258 (52.33%), Query Frame = 1

Query: 2   AGSRSSDGEKERRSS--VNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHIS 61
           + S   +   +R+SS      R +NET    L D  VLDC +C  PL++P +QC++GHI 
Sbjct: 4   SNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDGHIV 63

Query: 62  CSSCCSKVHNKCPS--CSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHE 121
           C+ C +KV NKCP   C LPIG  RC A+E+VLES  +PCQN+ +GC  + +  +++ HE
Sbjct: 64  CNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVSSHE 123

Query: 122 TLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYN---SRFTICLNSDDKYCI 181
             C Y  CSCP  +C+Y  S   +  HF +RH  ++  +S     S   + +N  +K  +
Sbjct: 124 KECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYSTVDVLINIKEKVSV 183

Query: 182 FQAENDGVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKSHG-NVLSLLSVAKE 241
                  +LFV+    E  G  VT++RI P +SE KKF Y +     G NV       K 
Sbjct: 184 LWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYESPEVKR 243

Query: 242 IQRWTEDPPSKGSLLIPN 251
           +       P    + +PN
Sbjct: 244 LLEVNSQIPDDSFMFVPN 261

BLAST of CmaCh01G003470 vs. Swiss-Prot
Match: SINL6_ARATH (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.1e-38
Identity = 88/255 (34.51%), Postives = 142/255 (55.69%), Query Frame = 1

Query: 4   SRSSDGEKERRSSVNSSRWK---NETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 63
           S  +  +K   SS++S   +   ++T   +LTD  +LDC IC   L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 123
           SSCC K+ NKCP+C+LP+G++RCRA+E+VLES+ +PC+ +  GC  T    + + HE +C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYN--SRFTICLNSDDKYCIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + + S+N  S     +   DK  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDGVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKS-HGNVLSLLS-VAKEIQR 243
              +LFV+    E  G  V++  I P + E  +F Y +   +  G  ++  S   K++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WTEDPPSKGSLLIPN 251
            +   P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

BLAST of CmaCh01G003470 vs. Swiss-Prot
Match: SINL4_ARATH (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 2.6e-37
Identity = 90/231 (38.96%), Postives = 127/231 (54.98%), Query Frame = 1

Query: 29  VILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEK 88
           V L +  VL+C  C  PL  P++QC NGH++C  CC K+  +C  C LPIG +RCRA+EK
Sbjct: 76  VTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEK 135

Query: 89  VLESIKMPCQNSRYGCK-TTTNLNQINEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRK 148
           V+++  + C N+ YGCK +TT  NQ+  HE +C + PCSCP+ DC+Y+   + L +HFR 
Sbjct: 136 VIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRA 195

Query: 149 RHQDSAKSIS-----YNSRFTICLNSDDKYCIFQAENDGVLFVLSYSFETFGDAVTLKRI 208
            H+ S   I+         F + L+S DK  IF  E  G LFV+     + G   T+  I
Sbjct: 196 THKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSHI 255

Query: 209 GPRSSE-KKFCYDIKAKSHGNVLSLLSVAKEIQ--RWTEDPPSKGSLLIPN 251
            P   E +KF   +      + L L    K IQ  R  E+ P +  LLIP+
Sbjct: 256 APMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

BLAST of CmaCh01G003470 vs. TrEMBL
Match: A0A0A0L9Z6_CUCSA (E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 5.8e-108
Identity = 190/269 (70.63%), Postives = 224/269 (83.27%), Query Frame = 1

Query: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60
           M  SRSSDG  ERRSSV S R  N T+ VI TDPQ+LDCYICC PLS+PV+QCENGHI+C
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120
           SSCC+K  NKCPSC+L IGY+RCRAIEKVLESIK+PCQN+ YGCKT   LN IN+HE+LC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180
            Y+PCSCPL +C++V S E LG HF K+H+DSAK  SYN+RFTICLN+ D + I +AEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240
           GVLF LSY+FE FG+AVT+ RIGP SSEKKFCY+IKAK+ G+VLSL S+AKEIQ   + P
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 270
           PSKGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 268

BLAST of CmaCh01G003470 vs. TrEMBL
Match: A0A061EGM7_THECC (E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-65
Identity = 122/265 (46.04%), Postives = 173/265 (65.28%), Query Frame = 1

Query: 10  EKERRSSVNSSRWKNET-----MKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCC 69
           E+E  S         ET     + + LTDP+VLDC IC   L++PV+QCENGHI+CS CC
Sbjct: 71  EEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEALTIPVFQCENGHIACSICC 130

Query: 70  SKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQP 129
            K+ NKCPSC+ PIGY RCRAIEKVLES+K+ CQN++YGC+     +   +HE +CPY P
Sbjct: 131 IKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCREAFRYSIKQKHEKICPYAP 190

Query: 130 CSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLF 189
           CSCPL+DC++  S++ L  HFR +H+ SA    Y+   +I +  D+++CI Q E DG LF
Sbjct: 191 CSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISITVGIDERFCILQEEQDGSLF 250

Query: 190 VLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAK--SHGNVLSLLSVAKEIQRWTEDPPS 249
           +L+   ET G+ VTL RIGP S E+ F YD+ AK  +  + + L S  K   +  ++PPS
Sbjct: 251 ILNNKVETLGNVVTLSRIGP-SEERGFFYDLMAKALTEASTVRLQSFTKSTPKQVDNPPS 310

Query: 250 KGSLLIPNEFFGSSSAQTILEIRIW 268
            G LL+P++ F  +S +  +++R+W
Sbjct: 311 LGFLLVPSQ-FSCNSRKLKMDLRLW 333

BLAST of CmaCh01G003470 vs. TrEMBL
Match: A0A061EG86_THECC (E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-65
Identity = 122/265 (46.04%), Postives = 173/265 (65.28%), Query Frame = 1

Query: 10  EKERRSSVNSSRWKNET-----MKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCC 69
           E+E  S         ET     + + LTDP+VLDC IC   L++PV+QCENGHI+CS CC
Sbjct: 71  EEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEALTIPVFQCENGHIACSICC 130

Query: 70  SKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQP 129
            K+ NKCPSC+ PIGY RCRAIEKVLES+K+ CQN++YGC+     +   +HE +CPY P
Sbjct: 131 IKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCREAFRYSIKQKHEKICPYAP 190

Query: 130 CSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLF 189
           CSCPL+DC++  S++ L  HFR +H+ SA    Y+   +I +  D+++CI Q E DG LF
Sbjct: 191 CSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISITVGIDERFCILQEEQDGSLF 250

Query: 190 VLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAK--SHGNVLSLLSVAKEIQRWTEDPPS 249
           +L+   ET G+ VTL RIGP S E+ F YD+ AK  +  + + L S  K   +  ++PPS
Sbjct: 251 ILNNKVETLGNVVTLSRIGP-SEERGFFYDLMAKALTEASTVRLQSFTKSTPKQVDNPPS 310

Query: 250 KGSLLIPNEFFGSSSAQTILEIRIW 268
            G LL+P++ F  +S +  +++R+W
Sbjct: 311 LGFLLVPSQ-FSCNSRKLKMDLRLW 333

BLAST of CmaCh01G003470 vs. TrEMBL
Match: W9S0Z7_9ROSA (E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_027128 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 8.7e-64
Identity = 129/253 (50.99%), Postives = 165/253 (65.22%), Query Frame = 1

Query: 4   SRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSC 63
           S  ++ E  R  S NS R    ++ V LTDP VLDC IC   L+VPV+QCENGHI+CSSC
Sbjct: 93  SADANQEDPRPLSTNSDR----SISVFLTDPDVLDCCICFEALTVPVFQCENGHIACSSC 152

Query: 64  CSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQ 123
           C+K+ NKCPSCS PIGY RCRAIEKVLES+K+ CQN +YGCK   + ++I +HET C + 
Sbjct: 153 CTKLGNKCPSCSWPIGYNRCRAIEKVLESVKIACQNKQYGCKEMVSYSEIKDHETTCTHA 212

Query: 124 PCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVL 183
           PCSCPL+ C+++ SA+ L  HFR +H DSA    YN  FT+ LN  DK+ + Q  + G L
Sbjct: 213 PCSCPLTSCNFISSAKQLYQHFRSQHADSAVLFQYNCDFTVTLNEGDKFLVLQERDGGGL 272

Query: 184 FVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSK 243
           F+L+   ET G+ V++  IGP S  K + Y++ AK  GN L L S A   Q   +   S 
Sbjct: 273 FILNNETETLGNLVSVSCIGPYSFGKSY-YNLLAKCDGNTLRLESFATCTQGQADYSLSS 332

Query: 244 GSLLIPNEFFGSS 257
             LLIP  FFGSS
Sbjct: 333 VYLLIPPGFFGSS 340

BLAST of CmaCh01G003470 vs. TrEMBL
Match: V4UW18_9ROSI (E3 ubiquitin-protein ligase OS=Citrus clementina GN=CICLE_v10012174mg PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.7e-62
Identity = 121/249 (48.59%), Postives = 159/249 (63.86%), Query Frame = 1

Query: 8   DGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCCSKV 67
           +GEK        SR  N    V +TDP+V DC IC   L+ PV+QCENGHI+CS+CCSK+
Sbjct: 59  NGEKADLDLAGPSR--NGPFSVTVTDPEVFDCPICYESLTAPVFQCENGHIACSTCCSKI 118

Query: 68  HNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSC 127
            NKCPSC  PIGY RCRAIEKVLES+K+ C+NS YGCK T +  + ++HE +CP+ PCSC
Sbjct: 119 MNKCPSCCTPIGYNRCRAIEKVLESVKVTCRNSNYGCKVTMSYGKKHDHEKICPHVPCSC 178

Query: 128 PLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLS 187
           P+ DC++V SA HL  HF  +H++SA    YN    + LN  +++ + Q E DGVLF+LS
Sbjct: 179 PIPDCNFVGSANHLYKHFSAKHKNSALHFLYNEVVEVTLNVKNRFIVLQEEGDGVLFILS 238

Query: 188 YSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSKGSLL 247
              ET G  +++  I P S +    Y I A   G+ +   S  K IQ   ++PPS G LL
Sbjct: 239 SRSETLGHVISVSCIAP-SCKGCLFYSIFAGPAGSTVRFQSFTKNIQNRVDNPPSTGFLL 298

Query: 248 IPNEFFGSS 257
           +P E FGSS
Sbjct: 299 VPIESFGSS 304

BLAST of CmaCh01G003470 vs. TAIR10
Match: AT5G37930.1 (AT5G37930.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 205.7 bits (522), Expect = 3.6e-53
Identity = 108/276 (39.13%), Postives = 161/276 (58.33%), Query Frame = 1

Query: 3   GSRSSDGEKE-----RR-----SSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQ 62
           GS S D ++E     RR     +S +SS  K+  + V L DP VLDC ICC PL +P++Q
Sbjct: 67  GSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQ 126

Query: 63  CENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQ 122
           C+NGH++C+ CC+KV N+CPSC+LPIGY+RCRA+EKV+E+ ++ C N++YGCK +T+   
Sbjct: 127 CDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGN 186

Query: 123 INEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKY 182
              HE +C + PCSCP+ DC Y    + L +H R  H+D   S  +N+R TI L+ + K 
Sbjct: 187 RFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKT 246

Query: 183 CIFQAENDGVLFVLSYSFETFGDAVTLKRIGPRS-SEKKFCYDIKAKSHGNVLSLLSVAK 242
            I Q ENDG + V+          V++  I P +    +    +   +  ++L    + K
Sbjct: 247 TILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVK 306

Query: 243 EIQRWTEDPPSKGSLLIPNEFFGSSSAQTILEIRIW 268
            IQ+ T + P  G +LIP+  F   S    L ++IW
Sbjct: 307 NIQKVTNEHPEDGFMLIPSYLF---SGNDNLNLQIW 339

BLAST of CmaCh01G003470 vs. TAIR10
Match: AT5G37890.1 (AT5G37890.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 161.0 bits (406), Expect = 1.0e-39
Identity = 88/268 (32.84%), Postives = 144/268 (53.73%), Query Frame = 1

Query: 3   GSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSS 62
           GS S   +K + SS ++++   +    +L D ++LDC IC    ++P++QC+NGH++CSS
Sbjct: 15  GSNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSS 74

Query: 63  CCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPY 122
           CC K++NKCP+C+ P+G+ RCRA+E VLESI +PC N++ GCK   +  +   HE  C +
Sbjct: 75  CCPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMF 134

Query: 123 QPCSCPLSDCSYVDSAEHLGSHFRKRHQD--SAKSISYNSRFTICLNSDDKYCIFQAEND 182
             C+CP  DC+Y  S + L +H+R  H +     +   +   ++ +N   K  I      
Sbjct: 135 SHCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLT 194

Query: 183 GVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKSHGNVLSLLS-VAKEIQRWTE 242
             LF +    E +G  VT+  I P S E  ++ Y +     G+ +   S   K + + + 
Sbjct: 195 NHLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQSPEVKRVLKLSF 254

Query: 243 DPPSKGSLLIPNEFFGSSSAQTILEIRI 267
             P +  +LIPN          +LE+RI
Sbjct: 255 QTPQENFMLIPNSLLRGD----VLEMRI 278

BLAST of CmaCh01G003470 vs. TAIR10
Match: AT5G62800.1 (AT5G62800.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 161.0 bits (406), Expect = 1.0e-39
Identity = 92/258 (35.66%), Postives = 135/258 (52.33%), Query Frame = 1

Query: 2   AGSRSSDGEKERRSS--VNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHIS 61
           + S   +   +R+SS      R +NET    L D  VLDC +C  PL++P +QC++GHI 
Sbjct: 4   SNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDGHIV 63

Query: 62  CSSCCSKVHNKCPS--CSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHE 121
           C+ C +KV NKCP   C LPIG  RC A+E+VLES  +PCQN+ +GC  + +  +++ HE
Sbjct: 64  CNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVSSHE 123

Query: 122 TLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYN---SRFTICLNSDDKYCI 181
             C Y  CSCP  +C+Y  S   +  HF +RH  ++  +S     S   + +N  +K  +
Sbjct: 124 KECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYSTVDVLINIKEKVSV 183

Query: 182 FQAENDGVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKSHG-NVLSLLSVAKE 241
                  +LFV+    E  G  VT++RI P +SE KKF Y +     G NV       K 
Sbjct: 184 LWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYESPEVKR 243

Query: 242 IQRWTEDPPSKGSLLIPN 251
           +       P    + +PN
Sbjct: 244 LLEVNSQIPDDSFMFVPN 261

BLAST of CmaCh01G003470 vs. TAIR10
Match: AT5G37870.1 (AT5G37870.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 159.8 bits (403), Expect = 2.3e-39
Identity = 88/255 (34.51%), Postives = 142/255 (55.69%), Query Frame = 1

Query: 4   SRSSDGEKERRSSVNSSRWK---NETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 63
           S  +  +K   SS++S   +   ++T   +LTD  +LDC IC   L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 123
           SSCC K+ NKCP+C+LP+G++RCRA+E+VLES+ +PC+ +  GC  T    + + HE +C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYN--SRFTICLNSDDKYCIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + + S+N  S     +   DK  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDGVLFVLSYSFETFGDAVTLKRIGPRSSE-KKFCYDIKAKS-HGNVLSLLS-VAKEIQR 243
              +LFV+    E  G  V++  I P + E  +F Y +   +  G  ++  S   K++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WTEDPPSKGSLLIPN 251
            +   P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

BLAST of CmaCh01G003470 vs. TAIR10
Match: AT1G66650.1 (AT1G66650.1 Protein with RING/U-box and TRAF-like domains)

HSP 1 Score: 157.1 bits (396), Expect = 1.5e-38
Identity = 90/231 (38.96%), Postives = 127/231 (54.98%), Query Frame = 1

Query: 29  VILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEK 88
           V L +  VL+C  C  PL  P++QC NGH++C  CC K+  +C  C LPIG +RCRA+EK
Sbjct: 76  VTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIGDVRCRAMEK 135

Query: 89  VLESIKMPCQNSRYGCK-TTTNLNQINEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRK 148
           V+++  + C N+ YGCK +TT  NQ+  HE +C + PCSCP+ DC+Y+   + L +HFR 
Sbjct: 136 VIKAGLVSCSNAIYGCKQSTTYGNQLQSHEKVCVFAPCSCPIKDCNYIGFYKDLINHFRA 195

Query: 149 RHQDSAKSIS-----YNSRFTICLNSDDKYCIFQAENDGVLFVLSYSFETFGDAVTLKRI 208
            H+ S   I+         F + L+S DK  IF  E  G LFV+     + G   T+  I
Sbjct: 196 THKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGSHGVYATVSHI 255

Query: 209 GPRSSE-KKFCYDIKAKSHGNVLSLLSVAKEIQ--RWTEDPPSKGSLLIPN 251
            P   E +KF   +      + L L    K IQ  R  E+ P +  LLIP+
Sbjct: 256 APMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIPS 306

BLAST of CmaCh01G003470 vs. NCBI nr
Match: gi|778679778|ref|XP_004140738.2| (PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus])

HSP 1 Score: 398.7 bits (1023), Expect = 8.3e-108
Identity = 190/269 (70.63%), Postives = 224/269 (83.27%), Query Frame = 1

Query: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60
           M  SRSSDG  ERRSSV S R  N T+ VI TDPQ+LDCYICC PLS+PV+QCENGHI+C
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120
           SSCC+K  NKCPSC+L IGY+RCRAIEKVLESIK+PCQN+ YGCKT   LN IN+HE+LC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180
            Y+PCSCPL +C++V S E LG HF K+H+DSAK  SYN+RFTICLN+ D + I +AEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240
           GVLF LSY+FE FG+AVT+ RIGP SSEKKFCY+IKAK+ G+VLSL S+AKEIQ   + P
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 270
           PSKGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 268

BLAST of CmaCh01G003470 vs. NCBI nr
Match: gi|659114528|ref|XP_008457096.1| (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SINA-like 10 [Cucumis melo])

HSP 1 Score: 299.3 bits (765), Expect = 6.8e-78
Identity = 143/215 (66.51%), Postives = 171/215 (79.53%), Query Frame = 1

Query: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60
           MA S+SSDG  ERRSSV S R  N TM VI TDPQ+LDCYIC   LS+P++QCEN HI+C
Sbjct: 1   MADSQSSDGGSERRSSVISRRGTNGTMNVIFTDPQILDCYICWNXLSIPIFQCENXHIAC 60

Query: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120
           SSCC+KV NKCPSC L IGY+RCRAIEKVLESIK+PC+N+ Y CKT  +LN IN+ ETLC
Sbjct: 61  SSCCTKVQNKCPSCILAIGYIRCRAIEKVLESIKLPCKNAIYRCKTAMDLNLINDQETLC 120

Query: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180
            Y+PCSCPL +C++V  AE L  HF +RH++SAK  SYN+RFTICLN+ D Y I +AE+D
Sbjct: 121 RYEPCSCPLDNCTFVGLAEQLYLHFSRRHKNSAKHFSYNTRFTICLNNGDTYRILKAEDD 180

Query: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDI 216
           GVLF LSY+FE FG+A+T+  IGP SSEKKF Y I
Sbjct: 181 GVLFFLSYTFEIFGNAITVNWIGPLSSEKKFSYKI 215

BLAST of CmaCh01G003470 vs. NCBI nr
Match: gi|590652270|ref|XP_007033104.1| (RING/U-box and TRAF-like domains, putative isoform 1 [Theobroma cacao])

HSP 1 Score: 257.3 bits (656), Expect = 3.0e-65
Identity = 122/265 (46.04%), Postives = 173/265 (65.28%), Query Frame = 1

Query: 10  EKERRSSVNSSRWKNET-----MKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCC 69
           E+E  S         ET     + + LTDP+VLDC IC   L++PV+QCENGHI+CS CC
Sbjct: 71  EEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEALTIPVFQCENGHIACSICC 130

Query: 70  SKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQP 129
            K+ NKCPSC+ PIGY RCRAIEKVLES+K+ CQN++YGC+     +   +HE +CPY P
Sbjct: 131 IKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCREAFRYSIKQKHEKICPYAP 190

Query: 130 CSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLF 189
           CSCPL+DC++  S++ L  HFR +H+ SA    Y+   +I +  D+++CI Q E DG LF
Sbjct: 191 CSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISITVGIDERFCILQEEQDGSLF 250

Query: 190 VLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAK--SHGNVLSLLSVAKEIQRWTEDPPS 249
           +L+   ET G+ VTL RIGP S E+ F YD+ AK  +  + + L S  K   +  ++PPS
Sbjct: 251 ILNNKVETLGNVVTLSRIGP-SEERGFFYDLMAKALTEASTVRLQSFTKSTPKQVDNPPS 310

Query: 250 KGSLLIPNEFFGSSSAQTILEIRIW 268
            G LL+P++ F  +S +  +++R+W
Sbjct: 311 LGFLLVPSQ-FSCNSRKLKMDLRLW 333

BLAST of CmaCh01G003470 vs. NCBI nr
Match: gi|590652274|ref|XP_007033105.1| (Ubiquitin-protein ligase, putative isoform 2 [Theobroma cacao])

HSP 1 Score: 257.3 bits (656), Expect = 3.0e-65
Identity = 122/265 (46.04%), Postives = 173/265 (65.28%), Query Frame = 1

Query: 10  EKERRSSVNSSRWKNET-----MKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSCC 69
           E+E  S         ET     + + LTDP+VLDC IC   L++PV+QCENGHI+CS CC
Sbjct: 71  EEEEESGAEQGAMSLETNRDGSISITLTDPEVLDCSICYEALTIPVFQCENGHIACSICC 130

Query: 70  SKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQP 129
            K+ NKCPSC+ PIGY RCRAIEKVLES+K+ CQN++YGC+     +   +HE +CPY P
Sbjct: 131 IKIRNKCPSCATPIGYSRCRAIEKVLESVKVSCQNTKYGCREAFRYSIKQKHEKICPYAP 190

Query: 130 CSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVLF 189
           CSCPL+DC++  S++ L  HFR +H+ SA    Y+   +I +  D+++CI Q E DG LF
Sbjct: 191 CSCPLADCNFEGSSKELYEHFRDKHKYSATRFQYDRTISITVGIDERFCILQEEQDGSLF 250

Query: 190 VLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAK--SHGNVLSLLSVAKEIQRWTEDPPS 249
           +L+   ET G+ VTL RIGP S E+ F YD+ AK  +  + + L S  K   +  ++PPS
Sbjct: 251 ILNNKVETLGNVVTLSRIGP-SEERGFFYDLMAKALTEASTVRLQSFTKSTPKQVDNPPS 310

Query: 250 KGSLLIPNEFFGSSSAQTILEIRIW 268
            G LL+P++ F  +S +  +++R+W
Sbjct: 311 LGFLLVPSQ-FSCNSRKLKMDLRLW 333

BLAST of CmaCh01G003470 vs. NCBI nr
Match: gi|703146966|ref|XP_010108933.1| (E3 ubiquitin-protein ligase SINA-like 10 [Morus notabilis])

HSP 1 Score: 251.9 bits (642), Expect = 1.3e-63
Identity = 129/253 (50.99%), Postives = 165/253 (65.22%), Query Frame = 1

Query: 4   SRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISCSSC 63
           S  ++ E  R  S NS R    ++ V LTDP VLDC IC   L+VPV+QCENGHI+CSSC
Sbjct: 93  SADANQEDPRPLSTNSDR----SISVFLTDPDVLDCCICFEALTVPVFQCENGHIACSSC 152

Query: 64  CSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQ 123
           C+K+ NKCPSCS PIGY RCRAIEKVLES+K+ CQN +YGCK   + ++I +HET C + 
Sbjct: 153 CTKLGNKCPSCSWPIGYNRCRAIEKVLESVKIACQNKQYGCKEMVSYSEIKDHETTCTHA 212

Query: 124 PCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAENDGVL 183
           PCSCPL+ C+++ SA+ L  HFR +H DSA    YN  FT+ LN  DK+ + Q  + G L
Sbjct: 213 PCSCPLTSCNFISSAKQLYQHFRSQHADSAVLFQYNCDFTVTLNEGDKFLVLQERDGGGL 272

Query: 184 FVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSK 243
           F+L+   ET G+ V++  IGP S  K + Y++ AK  GN L L S A   Q   +   S 
Sbjct: 273 FILNNETETLGNLVSVSCIGPYSFGKSY-YNLLAKCDGNTLRLESFATCTQGQADYSLSS 332

Query: 244 GSLLIPNEFFGSS 257
             LLIP  FFGSS
Sbjct: 333 VYLLIPPGFFGSS 340

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
SIL10_ARATH6.4e-5239.13E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana GN=At5g37930 PE... [more]
SINL7_ARATH1.8e-3832.84E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana GN=At5g37890 PE=... [more]
SIL11_ARATH1.8e-3835.66E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana GN=At5g62800 PE... [more]
SINL6_ARATH4.1e-3834.51Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana GN=At5g... [more]
SINL4_ARATH2.6e-3738.96E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana GN=At1g66650 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0L9Z6_CUCSA5.8e-10870.63E3 ubiquitin-protein ligase OS=Cucumis sativus GN=Csa_3G185660 PE=3 SV=1[more]
A0A061EGM7_THECC2.1e-6546.04E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1[more]
A0A061EG86_THECC2.1e-6546.04E3 ubiquitin-protein ligase OS=Theobroma cacao GN=TCM_019286 PE=3 SV=1[more]
W9S0Z7_9ROSA8.7e-6450.99E3 ubiquitin-protein ligase OS=Morus notabilis GN=L484_027128 PE=3 SV=1[more]
V4UW18_9ROSI3.7e-6248.59E3 ubiquitin-protein ligase OS=Citrus clementina GN=CICLE_v10012174mg PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G37930.13.6e-5339.13 Protein with RING/U-box and TRAF-like domains[more]
AT5G37890.11.0e-3932.84 Protein with RING/U-box and TRAF-like domains[more]
AT5G62800.11.0e-3935.66 Protein with RING/U-box and TRAF-like domains[more]
AT5G37870.12.3e-3934.51 Protein with RING/U-box and TRAF-like domains[more]
AT1G66650.11.5e-3838.96 Protein with RING/U-box and TRAF-like domains[more]
Match NameE-valueIdentityDescription
gi|778679778|ref|XP_004140738.2|8.3e-10870.63PREDICTED: E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus][more]
gi|659114528|ref|XP_008457096.1|6.8e-7866.51PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SINA-like 10 [Cucumi... [more]
gi|590652270|ref|XP_007033104.1|3.0e-6546.04RING/U-box and TRAF-like domains, putative isoform 1 [Theobroma cacao][more]
gi|590652274|ref|XP_007033105.1|3.0e-6546.04Ubiquitin-protein ligase, putative isoform 2 [Theobroma cacao][more]
gi|703146966|ref|XP_010108933.1|1.3e-6350.99E3 ubiquitin-protein ligase SINA-like 10 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR004162SINA-like
IPR008974TRAF-like
IPR013010Znf_SIAH
IPR013083Znf_RING/FYVE/PHD
IPR013323SIAH-type domain
IPR0181217-in-absentia-prot_TRAF-dom
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: Biological Process
TermDefinition
GO:0006511ubiquitin-dependent protein catabolic process
GO:0007275multicellular organism development
GO:0016567protein ubiquitination
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0004842ubiquitin-protein transferase activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0016874 ligase activity
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G003470.1CmaCh01G003470.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004162E3 ubiquitin-protein ligase SIN-likePANTHERPTHR10315SEVEN IN ABSENTIA HOMOLOGcoord: 10..267
score: 1.1
IPR008974TRAF-likeunknownSSF49599TRAF domain-likecoord: 92..267
score: 5.04
IPR013010Zinc finger, SIAH-typePROFILEPS51081ZF_SIAHcoord: 92..150
score: 13
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 26..98
score: 1.
IPR013323SIAH-type domainGENE3DG3DSA:3.90.890.10coord: 99..145
score: 2.0
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 81..238
score: 1.1
NoneNo IPR availablePANTHERPTHR10315:SF43E3 UBIQUITIN-PROTEIN LIGASEcoord: 10..267
score: 1.1

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None