CmaCh01G002580 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G002580
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionDelta(24)-sterol reductase
LocationCma_Chr01 : 1222533 .. 1224673 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAGACGTTGAGGCCTCCAAGCCACAAAAGAGGAAGAAAGGCTGGGTGGACTTTTTAGTTAATTTTCGATGGATTCTGGTTATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGAGACAAACGATCTGAGAGGAAGTCCTACAAGCAGCGCCAGATTGAACATGAAGAAAATGTCCAGATAGTTGTTGAACGCCTCAAAGAAAGAAACCCTTCGAAGGATGGTCTTGTCTGCACTGCCCGCAAGCCCTGGCTTGCGGTGGGAATGCGAAATGTTGACTACAAACGAGCACGACACTTCGAGGTTGATCTGTCGGCTTTCCGCAACATACTTGACATTGACAAGGAGAGAATGATAGCAAAAGTTGAGCCCCATGTGAACATGGGACAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTCGCAGTCGTTGCCGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAAGGAAGCTCCCATATCTATGGTCTTTTCTCTGATACAGTTGTGGCCTATGAAATTGTTCTTGCAGACGGCAGGGTTGTAAGGGCTACCAAGGATAATGAATACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGGACTCTGGGGTTTCTTGTGTCTGCTGAGATCAAACTTATACATATAAAGGAATATATGAAGCTTACATACACACCTGCCAAAGGCAACCTACAGGAGATTGGGCAGGCATATATGGATTCATTTACACCAAGAGATTTAGATCAAGATAACCCTGAGAAGGTCCCTGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAGCGAAGAGGCCAAGAAGAAAGGGAATGTGATCAACAGCATAGGCTGGTGGTTTAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAGGGGGAGTTCGTGGAGTACATCCCAACAAGAGAGTACTATCACAGACACACCAGATCTTTGTACTGGGAGGGAAAGCTTATTCTTCCATTTGCTGATCAATGGTGGTTCCGGTTTCTCTTAGGATGGATGATGCCTCCCAAGGTCTCTCTGCTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTGGTTCCTCTTCGACGAATTGGGGATACGTTGGAGTGGATCCACAAAGAGATGGAGGTAATTTCTTCTTGTGCTTTGAACATCCTACCATTCAAAATAGCAATTCAAATGAGAATTTGGGGTGTTTGGAGTGTTGAATTGGTAACAATTATACTACAGAACCAGGAGTCTCCCCTCATCTGTTGTTGTTTGTTTTGTCATTGCAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAAAACAATGGTGACTCCTGAACCAGGATTTGAACTTCAACTCAAGCAGGGTGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTATGCTCCCGGAGCTGTCCTAAGAGGAGAAGTTTACGACGGCGTTAAGGCAGTCAAGAACATGGGGAACTGGCTCATTGAAATCCATGGCTTCCAACCACAGCACTCAGTAACCGAGATGACCGAGGAAGAGTTTTGGAAAATGTTTGAGCCAGAACTTTATGAGGTGTGCCGAAAGAAGTATGGAGCCATAGGGACGTTCATGCATGTGTATTACAAATGTAAGAAGGGGAGGAAGACTGAGAAGGAAGTCCAGGAAGCTGAACGAGCTCAACCTGAAACTGCTTACGCTGATATAGAGCAGCCAGCTGAGTGATGCTTTTGCATTCATAAACAAAGTAGCATTCTTGTTTCTCCCCCTCCCACTTTGTGTTATGTTCTCTGCATTTATTCTTCTTTGAAATTTCACTGTTGCCTTCCCACTCTTTGTATCTTTCACTCTCATTAACACTTTCACGTGTATGTTAATTATGGTATTTGTTTTGTATATTGTAATGCCAAAACTGAAAAAATCTCGATTTCAGTTTATTATTATTATTATTTTGAGTTCAATTTAGAGATCCCATCTCGACTGTGGAGGAGAATAAAACATTTTTATAAGGGT

mRNA sequence

ATGTCAGACGTTGAGGCCTCCAAGCCACAAAAGAGGAAGAAAGGCTGGGTGGACTTTTTAGTTAATTTTCGATGGATTCTGGTTATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGAGACAAACGATCTGAGAGGAAGTCCTACAAGCAGCGCCAGATTGAACATGAAGAAAATGTCCAGATAGTTGTTGAACGCCTCAAAGAAAGAAACCCTTCGAAGGATGGTCTTGTCTGCACTGCCCGCAAGCCCTGGCTTGCGGTGGGAATGCGAAATGTTGACTACAAACGAGCACGACACTTCGAGGTTGATCTGTCGGCTTTCCGCAACATACTTGACATTGACAAGGAGAGAATGATAGCAAAAGTTGAGCCCCATGTGAACATGGGACAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTCGCAGTCGTTGCCGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAAGGAAGCTCCCATATCTATGGTCTTTTCTCTGATACAGTTGTGGCCTATGAAATTGTTCTTGCAGACGGCAGGGTTGTAAGGGCTACCAAGGATAATGAATACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGGACTCTGGGGTTTCTTGTGTCTGCTGAGATCAAACTTATACATATAAAGGAATATATGAAGCTTACATACACACCTGCCAAAGGCAACCTACAGGAGATTGGGCAGGCATATATGGATTCATTTACACCAAGAGATTTAGATCAAGATAACCCTGAGAAGGTCCCTGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAGCGAAGAGGCCAAGAAGAAAGGGAATGTGATCAACAGCATAGGCTGGTGGTTTAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAGGGGGAGTTCGTGGAGTACATCCCAACAAGAGAGTACTATCACAGACACACCAGATCTTTGTACTGGGAGGGAAAGCTTATTCTTCCATTTGCTGATCAATGGTGGTTCCGGTTTCTCTTAGGATGGATGATGCCTCCCAAGGTCTCTCTGCTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTGGTTCCTCTTCGACGAATTGGGGATACGTTGGAGTGGATCCACAAAGAGATGGAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAAAACAATGGTGACTCCTGAACCAGGATTTGAACTTCAACTCAAGCAGGGTGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTATGCTCCCGGAGCTGTCCTAAGAGGAGAAGTTTACGACGGCGTTAAGGCAGTCAAGAACATGGGGAACTGGCTCATTGAAATCCATGGCTTCCAACCACAGCACTCAGTAACCGAGATGACCGAGGAAGAGTTTTGGAAAATGTTTGAGCCAGAACTTTATGAGGTGTGCCGAAAGAAGTATGGAGCCATAGGGACGTTCATGCATGTGTATTACAAATGTAAGAAGGGGAGGAAGACTGAGAAGGAAGTCCAGGAAGCTGAACGAGCTCAACCTGAAACTGCTTACGCTGATATAGAGCAGCCAGCTGAGTGATGCTTTTGCATTCATAAACAAAGTAGCATTCTTGTTTCTCCCCCTCCCACTTTGTGTTATGTTCTCTGCATTTATTCTTCTTTGAAATTTCACTGTTGCCTTCCCACTCTTTGTATCTTTCACTCTCATTAACACTTTCACGTGTATGTTAATTATGGTATTTGTTTTGTATATTGTAATGCCAAAACTGAAAAAATCTCGATTTCAGTTTATTATTATTATTATTTTGAGTTCAATTTAGAGATCCCATCTCGACTGTGGAGGAGAATAAAACATTTTTATAAGGGT

Coding sequence (CDS)

ATGTCAGACGTTGAGGCCTCCAAGCCACAAAAGAGGAAGAAAGGCTGGGTGGACTTTTTAGTTAATTTTCGATGGATTCTGGTTATTTTTGTAGTTCTTCCGTTCTCATTCAGTTTCTACTTCATGCAATATCTTGGAGACAAACGATCTGAGAGGAAGTCCTACAAGCAGCGCCAGATTGAACATGAAGAAAATGTCCAGATAGTTGTTGAACGCCTCAAAGAAAGAAACCCTTCGAAGGATGGTCTTGTCTGCACTGCCCGCAAGCCCTGGCTTGCGGTGGGAATGCGAAATGTTGACTACAAACGAGCACGACACTTCGAGGTTGATCTGTCGGCTTTCCGCAACATACTTGACATTGACAAGGAGAGAATGATAGCAAAAGTTGAGCCCCATGTGAACATGGGACAAATATCTAGAGCCACAATTCCCATGAACCTTTCACTCGCAGTCGTTGCCGAGCTCGATGATCTTACTGTTGGTGGCCTCATCAATGGCTATGGCATTGAAGGAAGCTCCCATATCTATGGTCTTTTCTCTGATACAGTTGTGGCCTATGAAATTGTTCTTGCAGACGGCAGGGTTGTAAGGGCTACCAAGGATAATGAATACTCTGATCTTTTCTATGCCATCCCATGGTCTCAAGGGACTCTGGGGTTTCTTGTGTCTGCTGAGATCAAACTTATACATATAAAGGAATATATGAAGCTTACATACACACCTGCCAAAGGCAACCTACAGGAGATTGGGCAGGCATATATGGATTCATTTACACCAAGAGATTTAGATCAAGATAACCCTGAGAAGGTCCCTGACTTCGTAGAAACCATGATTTATTCTCCTACCGAAGCCGTGTGCATGACAGGAAGATATGCATCCAGCGAAGAGGCCAAGAAGAAAGGGAATGTGATCAACAGCATAGGCTGGTGGTTTAAGCCCTGGTTTTACCAGCATGCACAAAAAGCACTGAAAAAGGGGGAGTTCGTGGAGTACATCCCAACAAGAGAGTACTATCACAGACACACCAGATCTTTGTACTGGGAGGGAAAGCTTATTCTTCCATTTGCTGATCAATGGTGGTTCCGGTTTCTCTTAGGATGGATGATGCCTCCCAAGGTCTCTCTGCTAAAGGCTACTCAAGGTGAGGCTATCAGAAACTATTACCATGAGATGCATATTGTTCAGGACATGCTGGTTCCTCTTCGACGAATTGGGGATACGTTGGAGTGGATCCACAAAGAGATGGAGGTGTACCCATTATGGCTCTGCCCACACAGATTGTTCAAGCCCCCAATGAAAACAATGGTGACTCCTGAACCAGGATTTGAACTTCAACTCAAGCAGGGTGACACCCCCTACGCTCAGATGTACACAGACATCGGAGTCTACTATGCTCCCGGAGCTGTCCTAAGAGGAGAAGTTTACGACGGCGTTAAGGCAGTCAAGAACATGGGGAACTGGCTCATTGAAATCCATGGCTTCCAACCACAGCACTCAGTAACCGAGATGACCGAGGAAGAGTTTTGGAAAATGTTTGAGCCAGAACTTTATGAGGTGTGCCGAAAGAAGTATGGAGCCATAGGGACGTTCATGCATGTGTATTACAAATGTAAGAAGGGGAGGAAGACTGAGAAGGAAGTCCAGGAAGCTGAACGAGCTCAACCTGAAACTGCTTACGCTGATATAGAGCAGCCAGCTGAGTGA

Protein sequence

MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYTPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKKGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPLWLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKAVKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKGRKTEKEVQEAERAQPETAYADIEQPAE
BLAST of CmaCh01G002580 vs. Swiss-Prot
Match: DIM_PEA (Delta(24)-sterol reductase OS=Pisum sativum GN=DIM PE=1 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 2.7e-270
Identity = 440/567 (77.60%), Postives = 500/567 (88.18%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EA    KRKK WVD+ V FRWILVIFVVLP SF+ YF+ YLGD RSE KS+K RQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVKFRWILVIFVVLPISFTLYFLTYLGDVRSEWKSFKTRQK 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQ VV RLK+RNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLS FRNILDI
Sbjct: 61  EHDENVQKVVNRLKKRNPSKDGLVCTARKPWVAVGMRNVDYKRARHFEVDLSPFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQI+R T+PMNL+LAVVAELDDLTVGGLINGYGIEGSSH YGLFS
Sbjct: 121 DKERMIARVEPLVNMGQITRVTVPMNLALAVVAELDDLTVGGLINGYGIEGSSHKYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVA+EI+LADG +V+ATKDNEYSDLFYAIPWSQGTLG LV+AE+KLI IKEYMKLTY 
Sbjct: 181 DTVVAFEIILADGSLVKATKDNEYSDLFYAIPWSQGTLGLLVAAEVKLIPIKEYMKLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL++I QAY DSF PRD DQDN EKVPDFVETMIYSPT AVCMTGRYAS EEAKKK
Sbjct: 241 PVVGNLKDIAQAYSDSFAPRDGDQDNDEKVPDFVETMIYSPTRAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GN IN++GWW+K WFYQHA+ ALKKG FVEYIPTREYYHRHTR LYWEGKLILPF DQ+ 
Sbjct: 301 GNKINNVGWWYKTWFYQHAETALKKGLFVEYIPTREYYHRHTRCLYWEGKLILPFGDQFS 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW  +EME+YP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWSDREMEIYPI 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPH+LFK P+KTM+ PE GFELQ +QGDT  AQM+TD+GVYYAPG VLRGEV+DG +A
Sbjct: 421 WLCPHKLFKLPIKTMIYPEAGFELQRRQGDTQNAQMFTDVGVYYAPGPVLRGEVFDGAEA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M +W+IE H FQPQ++V+E+ E+ FW+MF+  LYE CR+KYGA+GTFM VYYKCKKG
Sbjct: 481 VRKMESWMIENHCFQPQYAVSELNEKNFWRMFDAGLYEHCRRKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIEQPAE 568
           RKTEKEV+EAE+A  +TAY +++QP +
Sbjct: 541 RKTEKEVREAEQAHLDTAYPEVDQPPD 567

BLAST of CmaCh01G002580 vs. Swiss-Prot
Match: DIM_ARATH (Delta(24)-sterol reductase OS=Arabidopsis thaliana GN=DIM PE=1 SV=2)

HSP 1 Score: 890.6 bits (2300), Expect = 9.1e-258
Identity = 418/564 (74.11%), Postives = 489/564 (86.70%), Query Frame = 1

Query: 1   MSDVEASKPQ-KRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSD++    + KRKK WVD+ V FRWI+VIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILD 120
            EH+ENV+ V++RLK R+ SKDGLVCTARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+KE+M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YAS EEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDKS---KIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+H+EMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVK 480
           +WLCPH+LFK P+K  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG +
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKK 540
           AV+ M  WLIE HGFQPQ++V+E+ E+ FW+MF  ELYE CRKKY AIGTFM VYYK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVQEAERAQPETAYADIE 564
           GRKTEKEV+EAE+A  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CmaCh01G002580 vs. Swiss-Prot
Match: DHC24_RAT (Delta(24)-sterol reductase OS=Rattus norvegicus GN=Dhcr24 PE=2 SV=2)

HSP 1 Score: 376.7 bits (966), Expect = 4.4e-103
Identity = 220/533 (41.28%), Postives = 309/533 (57.97%), Query Frame = 1

Query: 12  RKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQIVVE 71
           R KG    L++ RW+ V   +LP S  F    Y+      + S   R   HE+ VQ + +
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVQDIQK 78

Query: 72  RLKE-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           +++E +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++L
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYTPAKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + P +G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKKGNVINSIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +NSIG +
Sbjct: 259 EAICEKFTHESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKAVKNMGNWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    ++ +  ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M  EEFW+MF+  LY   RK+ G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of CmaCh01G002580 vs. Swiss-Prot
Match: DHC24_MOUSE (Delta(24)-sterol reductase OS=Mus musculus GN=Dhcr24 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.7e-102
Identity = 219/533 (41.09%), Postives = 309/533 (57.97%), Query Frame = 1

Query: 12  RKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQIVVE 71
           R KG    L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ + +
Sbjct: 19  RVKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKE-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           +++E +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V+MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE++L
Sbjct: 139 PLVSMGQVTALLNSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELIL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYTPAKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + P +G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKKGNVINSIGWW 311
           +A  + FT      +N      FVE ++YS  EAV MTG      E  K    +NSIG +
Sbjct: 259 EAICEKFTRESQRLEN-----HFVEGLLYSLDEAVIMTGVMTDDVEPSK----LNSIGSY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCMSQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKAVKNMGNWLI 491
           P    +V P         +GD   A++Y DIG Y  P    R + ++    ++ +  ++ 
Sbjct: 439 PSQPGLVHP---------KGDE--AELYVDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M  EEFW+MF+  LY   RK+ G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLRKQLGCQDAFPEVYDKICKAAR 515

BLAST of CmaCh01G002580 vs. Swiss-Prot
Match: DHC24_HUMAN (Delta(24)-sterol reductase OS=Homo sapiens GN=DHCR24 PE=1 SV=2)

HSP 1 Score: 374.8 bits (961), Expect = 1.7e-102
Identity = 221/533 (41.46%), Postives = 308/533 (57.79%), Query Frame = 1

Query: 12  RKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEHEENVQIVVE 71
           R KG    L++ RW+ V   +LP S  F    Y+      + S   R   HE+ V+ + +
Sbjct: 19  RLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRL--HEQRVRDIQK 78

Query: 72  RLKE-RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDKERMIAKVE 131
           +++E +       +CT R  WL V +R   YK+  H  + ++   +IL++D ++ I +VE
Sbjct: 79  QVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKT-HKNIMINLM-DILEVDTKKQIVRVE 138

Query: 132 PHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDTVVAYEIVL 191
           P V MGQ++     +  +L V+ ELDDLTVGGLI G GIE SSH YGLF     AYE+VL
Sbjct: 139 PLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVL 198

Query: 192 ADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYTPAKGNLQEIG 251
           ADG  VR T  +E SDLFYA+PWS GTLGFLV+AEI++I  K+Y+KL + P +G      
Sbjct: 199 ADGSFVRCTP-SENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGL----- 258

Query: 252 QAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKKGNVINSIGWW 311
           +A    FT     Q+N      FVE ++YS  EAV MTG      E  K    +NSIG +
Sbjct: 259 EAICAKFTHESQRQEN-----HFVEGLLYSLDEAVIMTGVMTDEAEPSK----LNSIGNY 318

Query: 312 FKPWFYQHAQKALKKG-EFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFRFLLGWMM 371
           +KPWF++H +  LK   E +EYIP R YYHRHTRS++WE + I+PF +   FR+L GWM+
Sbjct: 319 YKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMV 378

Query: 372 PPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPLWLCPHRLFK 431
           PPK+SLLK TQGE +R  Y + H+VQDMLVP++ +   L     ++ VYP+WLCP  L  
Sbjct: 379 PPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFIL-- 438

Query: 432 PPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKAVKNMGNWLI 491
           P    +V P+ G E          A++Y DIG Y  P    R + ++    ++ +  ++ 
Sbjct: 439 PSQPGLVHPK-GNE----------AELYIDIGAYGEP----RVKHFEARSCMRQLEKFVR 498

Query: 492 EIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYK-CKKGR 542
            +HGFQ  ++   M  EEFW+MF+  LY   R+K G    F  VY K CK  R
Sbjct: 499 SVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAAR 515

BLAST of CmaCh01G002580 vs. TrEMBL
Match: A0A0A0KZ82_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G103350 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 553/567 (97.53%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           M+D+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHEENVQ VVERLK+RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKK
Sbjct: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN+IGWWFKPWFYQHAQK+LK GEFVEYIPTREYYHRHT+SLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIH+EMEVYPL
Sbjct: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRLFKPPMKTMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDGV+A
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+NM NWLIEIHGFQPQHSVTEM+E+EFWKMF+ ELYE CRKKYGAIGTFMHVYYKCKKG
Sbjct: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIEQPAE 568
           RKTEKEV+EAE+AQ ETAYAD+EQ  E
Sbjct: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 567

BLAST of CmaCh01G002580 vs. TrEMBL
Match: W9SYN1_9ROSA (Delta(24)-sterol reductase OS=Morus notabilis GN=L484_014984 PE=4 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 9.4e-286
Identity = 463/567 (81.66%), Postives = 520/567 (91.71%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EA    KRKK WVD+ V FRWILVIFVVLP SF+ YF+ YLGD +SERKS+KQRQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSERKSFKQRQK 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQ V++RLK+R+PSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVIKRLKQRDPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKDRMIARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADGRVVRATKDNE+SDLFYAIPWSQGTLG LV+AEIKLI +KEYM++TY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPVKEYMRVTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNLQE+GQ Y+DSF PRD DQDNP+KVPDFVETMIY+P EAVCMTGRYAS EEAKKK
Sbjct: 241 PVVGNLQELGQGYIDSFAPRDCDQDNPDKVPDFVETMIYNPAEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINS+GWWFKPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPG VLRGEV+DG  A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEVFDGAGA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M +WLI+ HGFQPQ++V+E++E+ FW+MF+  LYE CR+KYGA+GTFM VYYK KKG
Sbjct: 481 VRKMEDWLIKNHGFQPQYAVSELSEKSFWRMFDAGLYEHCRRKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIEQPAE 568
           RKTEKEV+EAE+A  ETAYA+++QPA+
Sbjct: 541 RKTEKEVREAEQAHLETAYAEVDQPAD 567

BLAST of CmaCh01G002580 vs. TrEMBL
Match: B9HHN5_POPTR (Diminuto family protein OS=Populus trichocarpa GN=POPTR_0008s08440g PE=4 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 1.3e-282
Identity = 466/561 (83.07%), Postives = 512/561 (91.27%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EA    KRKK WVD+ V FRWILVIFVVLP SF+ YF+ YLGD +SE KSYKQRQ 
Sbjct: 1   MSDLEAPLRPKRKKVWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSEMKSYKQRQK 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+ VV+RLKERNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQISRA++PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARVEPLVNMGQISRASVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI IKEYM+LTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPIKEYMRLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL+E+ QAY+DSF PRD DQDNPEKVPDFVETMIY+ TE V MTGRYAS EEAKKK
Sbjct: 241 PVVGNLKELAQAYIDSFAPRDGDQDNPEKVPDFVETMIYNSTEGVMMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN++GWWFKPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINNVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRLFK P+KTMV PEPGFE Q KQGDT YAQMYTD+GVYY+PG VLRGEV+DG  A
Sbjct: 421 WLCPHRLFKLPVKTMVYPEPGFEHQHKQGDTSYAQMYTDVGVYYSPGPVLRGEVFDGADA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M +WLIE  GFQPQ++V+E++E++FW+MF+ +LYE CRKKYGA+GTFM VYYK KKG
Sbjct: 481 VRRMEDWLIENRGFQPQYAVSELSEKKFWRMFDGDLYEHCRKKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVQEAERAQPETAYAD 562
           RKTEKEVQEAE+A  ETAYA+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAE 561

BLAST of CmaCh01G002580 vs. TrEMBL
Match: F6HGH6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01200 PE=4 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 1.3e-282
Identity = 457/563 (81.17%), Postives = 511/563 (90.76%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD++A    KRKK WVD+ V+FRWI+VIFVVLP SF+ YF+ YLGD RSE KS+KQRQ 
Sbjct: 54  MSDLQAPLRPKRKKIWVDYFVHFRWIIVIFVVLPISFTLYFLTYLGDVRSESKSFKQRQE 113

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH ENV+ V++RLKERNPS+DGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 114 EHNENVKKVIKRLKERNPSRDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 173

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+ EP VNMGQISR ++PMNL+LAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 174 DKERMIARCEPLVNMGQISRVSVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 233

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEI+LADGR+VRATKDNEYSDLFYAIPWSQGTLG LV+AEIKLI IKEYMKLTY 
Sbjct: 234 DTVVAYEIILADGRLVRATKDNEYSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMKLTYK 293

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL+++ Q Y+DSF PRD DQDNPEKVPDFVETMIY+PTEAVCMTGRYAS EEAKKK
Sbjct: 294 PVVGNLKDLAQGYLDSFAPRDGDQDNPEKVPDFVETMIYNPTEAVCMTGRYASKEEAKKK 353

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINS+GWW+KPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 354 GNVINSVGWWYKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 413

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H EMEVYP+
Sbjct: 414 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHHEMEVYPI 473

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPG VLRGE +DG +A
Sbjct: 474 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEQFDGAEA 533

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M NWLIE HGFQPQ++V+E+TE+ FW+MF+  LYE CRKKYGA+GTFM VYYKCKKG
Sbjct: 534 VRRMENWLIENHGFQPQYAVSELTEKNFWRMFDAGLYEHCRKKYGAVGTFMSVYYKCKKG 593

Query: 541 RKTEKEVQEAERAQPETAYADIE 564
           +KTEKEVQEAE+AQ ET YA+ +
Sbjct: 594 KKTEKEVQEAEQAQLETPYAEAD 616

BLAST of CmaCh01G002580 vs. TrEMBL
Match: A5BTG6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016141 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 1.1e-281
Identity = 456/563 (80.99%), Postives = 510/563 (90.59%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD++A    KRKK WVD+ V+FRWI+VIFVVLP SF+ YF+ YLGD RSE KS+KQRQ 
Sbjct: 1   MSDLQAPLRPKRKKIWVDYFVHFRWIIVIFVVLPISFTLYFLTYLGDVRSESKSFKQRQE 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH ENV+ V++RLKERNPS+DGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHNENVKKVIKRLKERNPSRDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+ EP VNMGQISR ++PMNL+LAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARCEPLVNMGQISRVSVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEI+LADGR+VRATKDNEYSDLFYAIPWSQGTLG LV+AEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIILADGRLVRATKDNEYSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMKLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL+++ Q Y+DSF PRD DQDN EKVPDFVETMIY+PTEAVCMTGRYAS EEAKKK
Sbjct: 241 PVVGNLKDLAQGYLDSFAPRDGDQDNXEKVPDFVETMIYNPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINS+GWW+KPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWYKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H EMEVYP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHHEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPG VLRGE +DG +A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEQFDGAEA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M NWLIE HGFQPQ++V+E+TE+ FW+MF+  LYE CRKKYGA+GTFM VYYKCKKG
Sbjct: 481 VRRMENWLIENHGFQPQYAVSELTEKNFWRMFDAGLYEHCRKKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIE 564
           +KTEKEVQEAE+AQ ET YA+ +
Sbjct: 541 KKTEKEVQEAEQAQLETPYAEAD 563

BLAST of CmaCh01G002580 vs. TAIR10
Match: AT3G19820.1 (AT3G19820.1 cell elongation protein / DWARF1 / DIMINUTO (DIM))

HSP 1 Score: 890.6 bits (2300), Expect = 5.1e-259
Identity = 418/564 (74.11%), Postives = 489/564 (86.70%), Query Frame = 1

Query: 1   MSDVEASKPQ-KRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQ 60
           MSD++    + KRKK WVD+ V FRWI+VIF+VLPFS +FYF+ YLGD  SE KS+++RQ
Sbjct: 1   MSDLQTPLVRPKRKKTWVDYFVKFRWIIVIFIVLPFSATFYFLIYLGDMWSESKSFEKRQ 60

Query: 61  IEHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILD 120
            EH+ENV+ V++RLK R+ SKDGLVCTARKPW+AVGMRNVDYKRARHFEVDL  FRNIL+
Sbjct: 61  KEHDENVKKVIKRLKGRDASKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLGEFRNILE 120

Query: 121 IDKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180
           I+KE+M A+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF
Sbjct: 121 INKEKMTARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLF 180

Query: 181 SDTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 240
           +DTV AYEIVLA G +VRAT+DNEYSDL+YAIPWSQGTLG LV+AEI+LI +KEYM+LTY
Sbjct: 181 ADTVEAYEIVLAGGELVRATRDNEYSDLYYAIPWSQGTLGLLVAAEIRLIKVKEYMRLTY 240

Query: 241 TPAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKK 300
            P KG+LQ + Q Y+DSF P+D D+    K+PDFVE M+Y+PTE V M G YAS EEAKK
Sbjct: 241 IPVKGDLQALAQGYIDSFAPKDGDKS---KIPDFVEGMVYNPTEGVMMVGTYASKEEAKK 300

Query: 301 KGNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQW 360
           KGN IN++GWWFKPWFYQHAQ ALKKG+FVEYIPTREYYHRHTR LYWEGKLILPF DQ+
Sbjct: 301 KGNKINNVGWWFKPWFYQHAQTALKKGQFVEYIPTREYYHRHTRCLYWEGKLILPFGDQF 360

Query: 361 WFRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYP 420
           WFR+LLGW+MPPKVSLLKATQGEAIRNYYH+MH++QDMLVPL ++GD LEW+H+EMEVYP
Sbjct: 361 WFRYLLGWLMPPKVSLLKATQGEAIRNYYHDMHVIQDMLVPLYKVGDALEWVHREMEVYP 420

Query: 421 LWLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVK 480
           +WLCPH+LFK P+K  + PEPGFE + +QGDT  AQMYTD+GVYYAPG VLRGE +DG +
Sbjct: 421 IWLCPHKLFKQPIKGQIYPEPGFEYENRQGDTEDAQMYTDVGVYYAPGCVLRGEEFDGSE 480

Query: 481 AVKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKK 540
           AV+ M  WLIE HGFQPQ++V+E+ E+ FW+MF  ELYE CRKKY AIGTFM VYYK KK
Sbjct: 481 AVRRMEKWLIENHGFQPQYAVSELDEKSFWRMFNGELYEECRKKYRAIGTFMSVYYKSKK 540

Query: 541 GRKTEKEVQEAERAQPETAYADIE 564
           GRKTEKEV+EAE+A  ETAYA+ +
Sbjct: 541 GRKTEKEVREAEQAHLETAYAEAD 561

BLAST of CmaCh01G002580 vs. NCBI nr
Match: gi|449438803|ref|XP_004137177.1| (PREDICTED: delta(24)-sterol reductase-like [Cucumis sativus])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 535/567 (94.36%), Postives = 553/567 (97.53%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           M+D+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQI
Sbjct: 1   MTDLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQI 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EHEENVQ VVERLK+RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHEENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYL 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKK
Sbjct: 241 PVKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN+IGWWFKPWFYQHAQK+LK GEFVEYIPTREYYHRHT+SLYWEGKLILPFADQWW
Sbjct: 301 GNVINNIGWWFKPWFYQHAQKSLKNGEFVEYIPTREYYHRHTKSLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRF LGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIH+EMEVYPL
Sbjct: 361 FRFFLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRLFKPPMKTMV+PEPGFELQLKQGDTPYAQMYTDIGVYYAPG VLRGEVYDGV+A
Sbjct: 421 WLCPHRLFKPPMKTMVSPEPGFELQLKQGDTPYAQMYTDIGVYYAPGPVLRGEVYDGVQA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+NM NWLIEIHGFQPQHSVTEM+E+EFWKMF+ ELYE CRKKYGAIGTFMHVYYKCKKG
Sbjct: 481 VRNMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIEQPAE 568
           RKTEKEV+EAE+AQ ETAYAD+EQ  E
Sbjct: 541 RKTEKEVEEAEKAQAETAYADVEQAGE 567

BLAST of CmaCh01G002580 vs. NCBI nr
Match: gi|659120557|ref|XP_008460246.1| (PREDICTED: delta(24)-sterol reductase-like [Cucumis melo])

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 533/565 (94.34%), Postives = 549/565 (97.17%), Query Frame = 1

Query: 3   DVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQIEH 62
           D+EASKPQKRKKGWVD LVNFRWI+VIFVVLPFSFSFYF+QYLGDKRSERKSYKQRQIEH
Sbjct: 2   DLEASKPQKRKKGWVDILVNFRWIIVIFVVLPFSFSFYFIQYLGDKRSERKSYKQRQIEH 61

Query: 63  EENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK 122
           EENVQ VVERLK+RNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK
Sbjct: 62  EENVQKVVERLKQRNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDIDK 121

Query: 123 ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 182
           ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT
Sbjct: 122 ERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFSDT 181

Query: 183 VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYTPA 242
           VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTY P 
Sbjct: 182 VVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYLPV 241

Query: 243 KGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKKGN 302
           KG LQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYAS EEAKKKGN
Sbjct: 242 KGKLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASKEEAKKKGN 301

Query: 303 VINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR 362
           VINSIGWWFKPWFYQHAQKAL KGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR
Sbjct: 302 VINSIGWWFKPWFYQHAQKALIKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWWFR 361

Query: 363 FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPLWL 422
           FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIH+EMEVYPLWL
Sbjct: 362 FLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHREMEVYPLWL 421

Query: 423 CPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKAVK 482
           CPHRLFKPPMKTMV+PEPGFELQ+KQGDT YAQMYTDIGVYYAPG VLRGEVYDG +AV+
Sbjct: 422 CPHRLFKPPMKTMVSPEPGFELQIKQGDTSYAQMYTDIGVYYAPGPVLRGEVYDGAQAVR 481

Query: 483 NMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKGRK 542
           NM NWLIEIHGFQPQHSVTEM+E+EFWKMF+ ELYE CRKKYGAIGTFMHVYYKCKKGRK
Sbjct: 482 NMENWLIEIHGFQPQHSVTEMSEKEFWKMFDSELYEACRKKYGAIGTFMHVYYKCKKGRK 541

Query: 543 TEKEVQEAERAQPETAYADIEQPAE 568
           TEKE++EAE AQ ETAYAD+EQ +E
Sbjct: 542 TEKEIEEAENAQAETAYADVEQASE 566

BLAST of CmaCh01G002580 vs. NCBI nr
Match: gi|703159643|ref|XP_010112301.1| (Delta(24)-sterol reductase [Morus notabilis])

HSP 1 Score: 990.3 bits (2559), Expect = 1.4e-285
Identity = 463/567 (81.66%), Postives = 520/567 (91.71%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EA    KRKK WVD+ V FRWILVIFVVLP SF+ YF+ YLGD +SERKS+KQRQ 
Sbjct: 1   MSDLEAPLRPKRKKIWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSERKSFKQRQK 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENVQ V++RLK+R+PSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHDENVQKVIKRLKQRDPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DK+RMIA+VEP VNMGQISRAT+PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKDRMIARVEPLVNMGQISRATVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADGRVVRATKDNE+SDLFYAIPWSQGTLG LV+AEIKLI +KEYM++TY 
Sbjct: 181 DTVVAYEIVLADGRVVRATKDNEFSDLFYAIPWSQGTLGLLVAAEIKLIPVKEYMRVTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNLQE+GQ Y+DSF PRD DQDNP+KVPDFVETMIY+P EAVCMTGRYAS EEAKKK
Sbjct: 241 PVVGNLQELGQGYIDSFAPRDCDQDNPDKVPDFVETMIYNPAEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINS+GWWFKPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYP+
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPG VLRGEV+DG  A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEVFDGAGA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M +WLI+ HGFQPQ++V+E++E+ FW+MF+  LYE CR+KYGA+GTFM VYYK KKG
Sbjct: 481 VRKMEDWLIKNHGFQPQYAVSELSEKSFWRMFDAGLYEHCRRKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIEQPAE 568
           RKTEKEV+EAE+A  ETAYA+++QPA+
Sbjct: 541 RKTEKEVREAEQAHLETAYAEVDQPAD 567

BLAST of CmaCh01G002580 vs. NCBI nr
Match: gi|224098551|ref|XP_002311215.1| (diminuto family protein [Populus trichocarpa])

HSP 1 Score: 979.9 bits (2532), Expect = 1.8e-282
Identity = 466/561 (83.07%), Postives = 512/561 (91.27%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD+EA    KRKK WVD+ V FRWILVIFVVLP SF+ YF+ YLGD +SE KSYKQRQ 
Sbjct: 1   MSDLEAPLRPKRKKVWVDYFVQFRWILVIFVVLPISFTLYFLTYLGDVKSEMKSYKQRQK 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH+ENV+ VV+RLKERNPSKDGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNIL+I
Sbjct: 61  EHDENVKKVVKRLKERNPSKDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILEI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+VEP VNMGQISRA++PMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARVEPLVNMGQISRASVPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEIVLADG+VVRATKDNEYSDLFYAIPWSQGTLG LVSAEIKLI IKEYM+LTY 
Sbjct: 181 DTVVAYEIVLADGQVVRATKDNEYSDLFYAIPWSQGTLGLLVSAEIKLIPIKEYMRLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL+E+ QAY+DSF PRD DQDNPEKVPDFVETMIY+ TE V MTGRYAS EEAKKK
Sbjct: 241 PVVGNLKELAQAYIDSFAPRDGDQDNPEKVPDFVETMIYNSTEGVMMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVIN++GWWFKPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINNVGWWFKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H+EMEVYPL
Sbjct: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHQEMEVYPL 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRLFK P+KTMV PEPGFE Q KQGDT YAQMYTD+GVYY+PG VLRGEV+DG  A
Sbjct: 421 WLCPHRLFKLPVKTMVYPEPGFEHQHKQGDTSYAQMYTDVGVYYSPGPVLRGEVFDGADA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M +WLIE  GFQPQ++V+E++E++FW+MF+ +LYE CRKKYGA+GTFM VYYK KKG
Sbjct: 481 VRRMEDWLIENRGFQPQYAVSELSEKKFWRMFDGDLYEHCRKKYGAVGTFMSVYYKSKKG 540

Query: 541 RKTEKEVQEAERAQPETAYAD 562
           RKTEKEVQEAE+A  ETAYA+
Sbjct: 541 RKTEKEVQEAEQAHLETAYAE 561

BLAST of CmaCh01G002580 vs. NCBI nr
Match: gi|359473657|ref|XP_002271846.2| (PREDICTED: delta(24)-sterol reductase [Vitis vinifera])

HSP 1 Score: 979.9 bits (2532), Expect = 1.8e-282
Identity = 457/563 (81.17%), Postives = 511/563 (90.76%), Query Frame = 1

Query: 1   MSDVEASKPQKRKKGWVDFLVNFRWILVIFVVLPFSFSFYFMQYLGDKRSERKSYKQRQI 60
           MSD++A    KRKK WVD+ V+FRWI+VIFVVLP SF+ YF+ YLGD RSE KS+KQRQ 
Sbjct: 1   MSDLQAPLRPKRKKIWVDYFVHFRWIIVIFVVLPISFTLYFLTYLGDVRSESKSFKQRQE 60

Query: 61  EHEENVQIVVERLKERNPSKDGLVCTARKPWLAVGMRNVDYKRARHFEVDLSAFRNILDI 120
           EH ENV+ V++RLKERNPS+DGLVCTARKPW+AVGMRNVDYKRARHFEVDLSAFRNILDI
Sbjct: 61  EHNENVKKVIKRLKERNPSRDGLVCTARKPWIAVGMRNVDYKRARHFEVDLSAFRNILDI 120

Query: 121 DKERMIAKVEPHVNMGQISRATIPMNLSLAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180
           DKERMIA+ EP VNMGQISR ++PMNL+LAVVAELDDLTVGGLINGYGIEGSSHIYGLFS
Sbjct: 121 DKERMIARCEPLVNMGQISRVSVPMNLALAVVAELDDLTVGGLINGYGIEGSSHIYGLFS 180

Query: 181 DTVVAYEIVLADGRVVRATKDNEYSDLFYAIPWSQGTLGFLVSAEIKLIHIKEYMKLTYT 240
           DTVVAYEI+LADGR+VRATKDNEYSDLFYAIPWSQGTLG LV+AEIKLI IKEYMKLTY 
Sbjct: 181 DTVVAYEIILADGRLVRATKDNEYSDLFYAIPWSQGTLGLLVAAEIKLIPIKEYMKLTYK 240

Query: 241 PAKGNLQEIGQAYMDSFTPRDLDQDNPEKVPDFVETMIYSPTEAVCMTGRYASSEEAKKK 300
           P  GNL+++ Q Y+DSF PRD DQDNPEKVPDFVETMIY+PTEAVCMTGRYAS EEAKKK
Sbjct: 241 PVVGNLKDLAQGYLDSFAPRDGDQDNPEKVPDFVETMIYNPTEAVCMTGRYASKEEAKKK 300

Query: 301 GNVINSIGWWFKPWFYQHAQKALKKGEFVEYIPTREYYHRHTRSLYWEGKLILPFADQWW 360
           GNVINS+GWW+KPWFYQHAQ ALKKGEFVEYIPTREYYHRHTR LYWEGKLILPFADQWW
Sbjct: 301 GNVINSVGWWYKPWFYQHAQTALKKGEFVEYIPTREYYHRHTRCLYWEGKLILPFADQWW 360

Query: 361 FRFLLGWMMPPKVSLLKATQGEAIRNYYHEMHIVQDMLVPLRRIGDTLEWIHKEMEVYPL 420
           FRFL GW+MPPKVSLLKATQGEAIRNYYHEMH++QDMLVPL ++GD LEW+H EMEVYP+
Sbjct: 361 FRFLFGWLMPPKVSLLKATQGEAIRNYYHEMHVIQDMLVPLYKVGDALEWVHHEMEVYPI 420

Query: 421 WLCPHRLFKPPMKTMVTPEPGFELQLKQGDTPYAQMYTDIGVYYAPGAVLRGEVYDGVKA 480
           WLCPHRL+K P+KTM+ PEPGFEL  +QGDT YAQMYTD+GVYYAPG VLRGE +DG +A
Sbjct: 421 WLCPHRLYKLPVKTMIYPEPGFELHRRQGDTHYAQMYTDVGVYYAPGPVLRGEQFDGAEA 480

Query: 481 VKNMGNWLIEIHGFQPQHSVTEMTEEEFWKMFEPELYEVCRKKYGAIGTFMHVYYKCKKG 540
           V+ M NWLIE HGFQPQ++V+E+TE+ FW+MF+  LYE CRKKYGA+GTFM VYYKCKKG
Sbjct: 481 VRRMENWLIENHGFQPQYAVSELTEKNFWRMFDAGLYEHCRKKYGAVGTFMSVYYKCKKG 540

Query: 541 RKTEKEVQEAERAQPETAYADIE 564
           +KTEKEVQEAE+AQ ET YA+ +
Sbjct: 541 KKTEKEVQEAEQAQLETPYAEAD 563

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
DIM_PEA2.7e-27077.60Delta(24)-sterol reductase OS=Pisum sativum GN=DIM PE=1 SV=1[more]
DIM_ARATH9.1e-25874.11Delta(24)-sterol reductase OS=Arabidopsis thaliana GN=DIM PE=1 SV=2[more]
DHC24_RAT4.4e-10341.28Delta(24)-sterol reductase OS=Rattus norvegicus GN=Dhcr24 PE=2 SV=2[more]
DHC24_MOUSE1.7e-10241.09Delta(24)-sterol reductase OS=Mus musculus GN=Dhcr24 PE=1 SV=1[more]
DHC24_HUMAN1.7e-10241.46Delta(24)-sterol reductase OS=Homo sapiens GN=DHCR24 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZ82_CUCSA0.0e+0094.36Uncharacterized protein OS=Cucumis sativus GN=Csa_4G103350 PE=4 SV=1[more]
W9SYN1_9ROSA9.4e-28681.66Delta(24)-sterol reductase OS=Morus notabilis GN=L484_014984 PE=4 SV=1[more]
B9HHN5_POPTR1.3e-28283.07Diminuto family protein OS=Populus trichocarpa GN=POPTR_0008s08440g PE=4 SV=1[more]
F6HGH6_VITVI1.3e-28281.17Putative uncharacterized protein OS=Vitis vinifera GN=VIT_01s0010g01200 PE=4 SV=... [more]
A5BTG6_VITVI1.1e-28180.99Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_016141 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G19820.15.1e-25974.11 cell elongation protein / DWARF1 / DIMINUTO (DIM)[more]
Match NameE-valueIdentityDescription
gi|449438803|ref|XP_004137177.1|0.0e+0094.36PREDICTED: delta(24)-sterol reductase-like [Cucumis sativus][more]
gi|659120557|ref|XP_008460246.1|0.0e+0094.34PREDICTED: delta(24)-sterol reductase-like [Cucumis melo][more]
gi|703159643|ref|XP_010112301.1|1.4e-28581.66Delta(24)-sterol reductase [Morus notabilis][more]
gi|224098551|ref|XP_002311215.1|1.8e-28283.07diminuto family protein [Populus trichocarpa][more]
gi|359473657|ref|XP_002271846.2|1.8e-28281.17PREDICTED: delta(24)-sterol reductase [Vitis vinifera][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR006094Oxid_FAD_bind_N
IPR016166FAD-bd_2
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2
Vocabulary: Molecular Function
TermDefinition
GO:0016491oxidoreductase activity
GO:0050660flavin adenine dinucleotide binding
GO:0016614oxidoreductase activity, acting on CH-OH group of donors
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006040 amino sugar metabolic process
biological_process GO:0043481 anthocyanin accumulation in tissues in response to UV light
biological_process GO:0008202 steroid metabolic process
biological_process GO:0016126 sterol biosynthetic process
biological_process GO:0005982 starch metabolic process
biological_process GO:0048767 root hair elongation
biological_process GO:0010075 regulation of meristem growth
biological_process GO:0000272 polysaccharide catabolic process
biological_process GO:0000271 polysaccharide biosynthetic process
biological_process GO:0009664 plant-type cell wall organization
biological_process GO:0009825 multidimensional cell growth
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0009932 cell tip growth
biological_process GO:0019344 cysteine biosynthetic process
biological_process GO:0048653 anther development
biological_process GO:0009826 unidimensional cell growth
biological_process GO:0009808 lignin metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006084 acetyl-CoA metabolic process
biological_process GO:0009834 plant-type secondary cell wall biogenesis
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0005575 cellular_component
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0050614 delta24-sterol reductase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
molecular_function GO:0008762 UDP-N-acetylmuramate dehydrogenase activity
molecular_function GO:0071949 FAD binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G002580.1CmaCh01G002580.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 109..198
score: 3.9
IPR016166FAD-binding, type 2PROFILEPS51387FAD_PCMHcoord: 52..231
score: 1
IPR016166FAD-binding, type 2unknownSSF56176FAD-binding/transporter-associated domain-likecoord: 104..229
score: 8.18
IPR016169CO dehydrogenase flavoprotein-like, FAD-binding, subdomain 2GENE3DG3DSA:3.30.465.10coord: 116..230
score: 1.8
NoneNo IPR availablePANTHERPTHR1080124-DEHYDROCHOLESTEROL REDUCTASEcoord: 1..551
score:
NoneNo IPR availablePANTHERPTHR10801:SF0DELTA(24)-STEROL REDUCTASEcoord: 1..551
score:

The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
CmaCh01G002580CmaCh17G009930Cucurbita maxima (Rimu)cmacmaB360
CmaCh01G002580CmaCh08G004970Cucurbita maxima (Rimu)cmacmaB440