CmaCh01G000680 (gene) Cucurbita maxima (Rimu)

NameCmaCh01G000680
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu))
DescriptionPhospholipase D
LocationCma_Chr01 : 294236 .. 297173 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCCTTTCACTCACTCTGCTACAAAATTGTTTCAAGTTATGACAGTTCAGGGAGAGCATACGTTCTTCCACGGCACGCTCGAAGTTACCGTCTTCCATGCAACAACATACACACCGTCATCACCTCTGGATGTAAGTCCTGTACCATAACAATGAGGTTAAATTACAAGTTTAGTCCCTGAACTTTCGTGGTTGTGTTTATAGGTCTCTAAACTTTCAACTTTGTCTAATACGTCGAATAATAGAATTTCTATATCGTCACGAAAATAACATCAACAGCTTTTTCTCTCTTGCAGTGTATATTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGGACAAACCACGAATATGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGTAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTGTATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGTGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAATACAAACAATCACCTATCTTTGTTAAATTACAAACTTCAGTCCATGAATTTGTAACCGCTAATACTCTTTTGGTGATCAGGTAAGAGACCCTCAAACTGATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCGTCATCAAGAATGCTGGGATAATGAAAACACATGATGAAAATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGATGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGTGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAACAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCGACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGCACCATCCACGAGGCGTATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTCGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCGAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCGTAGAAGGTTTATGGTCTATGTCCATTCCAAGGTCATGATAGGTAAGTTTTCAAGTATTCCGAGCATCGTTTCTAAGGGGCATTTTAACAAACAAGTAGAGGGCAGTTTTAATAACATAATAATGTCAAAACAAACAAAGAATTGATTTATGAGTTTTGGATCTCATTTCAACAAACACAAATGACAAATCTATTATCCATAAAAGTGATGATGGGTTCATTACATATTCAAATTACTACGATTTAAAGCAAAAGTGTAGAAGAAGTAGAAACTAACCATAAACATGTGGGTGCTTGCAGTGGACGATTTGTACCTTCTGATCGGATCCGCAAACGTGAACCAAAGATCTATGGACGGAGAACGTGATACAGAAATAGCGATCGGATGCTACCAATTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTTGAAAGGGTTCGTTCAATAGCAGACGAATCGTGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTGGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGTCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAATTTTAATGGAGACTTGAATGGCGATGACCAAGCTCTGAGTTTGCATTTACTTGCTCGAAGATCCATGAATGAGATGACCCAACAGGGGAATTTGTGGTTTGGAG

mRNA sequence

TTCTTCCTTTCACTCACTCTGCTACAAAATTGTTTCAAGTTATGACAGTTCAGGGAGAGCATACGTTCTTCCACGGCACGCTCGAAGTTACCGTCTTCCATGCAACAACATACACACCGTCATCACCTCTGGATTGTATATTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGGACAAACCACGAATATGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGTAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTGTATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGTGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACCCTCAAACTGATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCGTCATCAAGAATGCTGGGATAATGAAAACACATGATGAAAATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGATGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGTGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAACAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCGACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGCACCATCCACGAGGCGTATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTCGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCGAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCGTAGAAGGTTTATGGTCTATGTCCATTCCAAGGTCATGATAGTGGACGATTTGTACCTTCTGATCGGATCCGCAAACGTGAACCAAAGATCTATGGACGGAGAACGTGATACAGAAATAGCGATCGGATGCTACCAATTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTTGAAAGGGTTCGTTCAATAGCAGACGAATCGTGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTGGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGTCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAATTTTAATGGAGACTTGAATGGCGATGACCAAGCTCTGAGTTTGCATTTACTTGCTCGAAGATCCATGAATGAGATGACCCAACAGGGGAATTTGTGGTTTGGAG

Coding sequence (CDS)

ATGACAGTTCAGGGAGAGCATACGTTCTTCCACGGCACGCTCGAAGTTACCGTCTTCCATGCAACAACATACACACCGTCATCACCTCTGGATTGTATATTTTCTGGAGGAAAGCAAAGCTATGTGACCATCAAGATAAACAACATAGAGGTTGCTCGGACAAACCACGAATATGACCGTGTTTGGAACCAAACATTCCGAGTTCTATGTGCGCATCCATTGACATCTACCATCACCATTACAATGAGAACAACTCGTTCTGTATTGGGAAGATTCCAGATCCAAGCCCAACAGATTTTGAAAGAATCAAGTTTCATCAATGGATTCTTTCCACTTCTTATGGAAAATGGGAAGCCGAGTCCAGAGCTCAGACTTAGATTCATGCTATGGTTTCAACCGGCAGTATATGAACTAAGTTGGAAAAAGGTGCTGGAAAATGGAGAGTACAAGGGGCTGAGAAATGTAACGTTTCCTCTAAGGTCCAACTGTCATGTGACTCTGTATCAAGATGCTCACCATGTCTCTACGTTTCAACCTCCGTTTCACGGTTCGAGTGCGCCAAGAAGGCTATGGGAAGATGTGTATAAGGCCATAGATAATGCAAAGCATTTGGTTTACATTGCAGGCTGGTCCTTCAATCCAAAGATGGTGCTGGTAAGAGACCCTCAAACTGATATACCCTATGCCTTGGGAGTAAAGCTTGGTGAGTTGTTGAAGCAGAAGGCAGAGGAAGGAGTAGCAGTGAGACTAATGATATGGGACGACGAGACATCCCTTCCCGTCATCAAGAATGCTGGGATAATGAAAACACATGATGAAAATGCTCGGGCATATTTCTCGAACTCGAAAGTTGTATGTAGGTTGTGCCCCAAATTACATCCTATGTCTCCACCAATTTTCTCTCATCATCAGAAGACCATAACAGTAGATGCTCAAACTCACATCAACGCAAGGAACAGGGAGATTATGAGTTTCATTGGGGGTCTAGATCTTTGTGATGGTCGCTATGACACAGAACAACATTCATTGTTCCATACTTTGAACACAGAATCCCACTGCTGTGATTTCTACCAAACGAGTATATCAGGAGCCAAGCTTCAAAAAGGAGGGCCAAGAGAGCCATGGCATGATGTTCATGCTCGTGTAACAGGTGAAGCTGCTTGGGATATACTAACAAACTTTGAACAACGATGGACTAAGCAATCAGATGCTTCGTTGCTAGTCCCAACAAGCATCTTACTAAAACTGATGCCCCCACTTGAATCAAACACAAACCCACAAAAGGATTGGAACGTACAAGTTTTTCGATCTATCGACCACTTGTCTGCCAGCCAAGTTTTCAGAAACTTGACGGTTGAACGCACCATCCACGAGGCGTATGTCGAAGCTATCAGAAGAGCCGAGAGATTTATTTACATCGAAAACCAATATTTCATAGGAGGGTGTCACTTGTGGGACAAAGATCAACACTGCGGATGCACGAATTTAATACCGATCGAGATTGCGCTCAAGGTGGCTAATAAGATCAAGGCAAGGGAGAAGTTCGCAGTTTATGTAGTGATTCCAATGTGGCCAGAAGGGCCACCAGAGAGTGAATCAGTAGAGGATATGCTACATTGGACCAGGCAGACAATGACAATGATGTATAGATTGATTGGAGAAGCAATCCAAGAAACTGGGGAGAAGGCCCATCCAAGAGACTACTTGAATTTCTTTTGCCTTGCGAACAGAGAAGAAGAGAGAAAGTGGGACTTTGTTCCCCCTCACAGTCCACAACATGCAACAGAATACTGGAGTGCCCAACACCGTAGAAGGTTTATGGTCTATGTCCATTCCAAGGTCATGATAGTGGACGATTTGTACCTTCTGATCGGATCCGCAAACGTGAACCAAAGATCTATGGACGGAGAACGTGATACAGAAATAGCGATCGGATGCTACCAATTAGAAAACGACGGAAAAGAATTGCCAAATGGAAGAGACATTTCAACATTCCGCTTGTCGCTTTGGTACGAACATACTCAACGATTCGAAGAGGTGTTCTTAAACCCCGAAAACTTGCAATGCGTTGAAAGGGTTCGTTCAATAGCAGACGAATCGTGGAAAATTTACAGTGGAGAAGAAGTTGCAGACATGAAAGGTGTTCATCTGGTAACGTACCCTGTGAAGGTAAAACAGGACGGATCGATTGAGGATTTGGAAGAAAATGGAGGTCATTTTCCTGATACTAAATGCCCCATTAAAGGAAGAAGATCAATGTTCTTGCCGCCCATTTTCACGACCTAA

Protein sequence

MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT
BLAST of CmaCh01G000680 vs. Swiss-Prot
Match: PLDA4_ARATH (Phospholipase D alpha 4 OS=Arabidopsis thaliana GN=PLDALPHA4 PE=2 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 2.2e-283
Identity = 466/779 (59.82%), Postives = 597/779 (76.64%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  +FHGTLE+T+F AT ++P  P +CI +  K +YVTIKIN  +VA+T+ EYDR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STITITMRTTRSVLGRFQIQAQQIL-KESSFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +TITIT++T  SVLGRF+I A+QIL   S+ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQ 180
               L+L+ ++WF+PA  E  W + LE   ++G+RN +FP RSNC V LYQDAHH +TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD +T+IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLH 300
           +GELLK+K+EEGVAVR+M+W+DETSLP+IKN G+M+T+ E A AYF N+ VVCRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTIT+D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTS- 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 --ILLKLMPPLESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAER 480
              L+ L  P E N    + WNVQV RSIDH+SA+++ R L VE+++H+ YV AIR+AER
Sbjct: 421 IRNLVNLTGPTEENN---RKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAER 480

Query: 481 FIYIENQYFIGGCHLWDK--DQHC-GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPE 540
           FIYIENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARE+FAVY+VIPMWPE
Sbjct: 481 FIYIENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPE 540

Query: 541 GPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFV 600
           GPPESE+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F 
Sbjct: 541 GPPESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFE 600

Query: 601 PPHSPQHATEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGC 660
              SP   T YW+AQ  RRFMVYVHSK+MIVDD Y+LIGSAN+NQRSMDG RDTEIAIGC
Sbjct: 601 AVSSPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGC 660

Query: 661 YQLENDGKELPNGRDISTFRLSLWYEHT----QRFEEVFLNPENLQCVERVRSIADESWK 720
           YQ   +     N  +I  +RLSLWYEHT       +     PE+L+CV  +R+I ++ W+
Sbjct: 661 YQTNTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWE 720

Query: 721 IYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           IYSG++V DM G+HLV YP+ V  DG++E++ +  G FPDTK  +KG+RS   PP+ TT
Sbjct: 721 IYSGDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

BLAST of CmaCh01G000680 vs. Swiss-Prot
Match: PLDA1_MAIZE (Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 3.3e-178
Identity = 333/810 (41.11%), Postives = 473/810 (58.40%), Query Frame = 1

Query: 11  HGTLEVTVFHATTY------TPSSPLDC------------IFSGGKQSYVTIKINNIEVA 70
           HGTL  T+F A +       T  +P               +  G  + Y T+ +    V 
Sbjct: 7   HGTLHATIFEAESLSNPHRATGGAPKFIRKLVEGIEDTVGVGKGATKIYATVDLEKARVG 66

Query: 71  RT----NHEYDRVWNQTFRVLCAHPLTS---TITITMRTTRSVLGRFQIQAQQILKESSF 130
           RT    N   +  W ++F + CAH       T+ I      S++GR  +  Q +L     
Sbjct: 67  RTRMISNEPVNPRWYESFHIYCAHMAADVIFTVKIDNSIGASLIGRAYLAVQDLLGGEE- 126

Query: 131 INGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHV 190
           I+ +  +  EN +P  + ++   L +     + +W + + + +Y G+    F  R  C V
Sbjct: 127 IDKWLEISDENREPVGDSKIHVKLQYFDVGKDRNWARGVRSTKYPGVPYTFFSQRQGCKV 186

Query: 191 TLYQDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD 250
           TLYQDAH    F P    +      P R WED++ AI  A+HL+YI GWS   ++ LVRD
Sbjct: 187 TLYQDAHVPDNFVPRIQLADGKNYEPHRCWEDIFDAISKAQHLIYITGWSVYTEITLVRD 246

Query: 251 PQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSN 310
                P    V LGELLK+KA EGV V +++WDD TS+ ++K  G+M THDE    YF  
Sbjct: 247 TNRPKPGG-DVTLGELLKRKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETANYFHG 306

Query: 311 SKVVCRLCPKLHPMS---------PPIFSHHQKTITVDAQT-HINARNREIMSFIGGLDL 370
           + V C LCP+    S           +F+HHQK + VD +  +  ++ R I+SFIGG+DL
Sbjct: 307 TDVNCVLCPRNPDDSGSFVQDLQISTMFTHHQKIVVVDHEMPNQGSQQRRIVSFIGGIDL 366

Query: 371 CDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDIL 430
           CDGRYDT+ HSLF TL+T  H  DF+Q +  G  ++KGGPREPWHD+H+R+ G  AWD+L
Sbjct: 367 CDGRYDTQYHSLFRTLDTVHHD-DFHQPNFEGGSIKKGGPREPWHDIHSRLEGPIAWDVL 426

Query: 431 TNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQ--KDWNVQVFRSIDHLSASQV--- 490
            NFEQRW KQ    LLV    L  ++ P      P+  + WNVQ+FRSID  +A      
Sbjct: 427 YNFEQRWRKQGGKDLLVRLRDLPDIIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPET 486

Query: 491 -----------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQHC 550
                       ++  ++R+I +AYV AIRRA+ FIYIENQYF+G  + W     K +  
Sbjct: 487 PEEAARAGLVSGKDQIIDRSIQDAYVNAIRRAKNFIYIENQYFLGSSYGWKPEGIKPEEI 546

Query: 551 GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRL 610
           G  +LIP E++LK+ +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY  
Sbjct: 547 GALHLIPKELSLKIVSKIEAGERFTVYVVVPMWPEGVPESASVQAILDWQRRTMEMMYTD 606

Query: 611 IGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRRRFMVYVH 670
           I +A++  G +A+P+DYL FFCL NRE +++ ++ P   P+  T+Y  AQ  RRFM+YVH
Sbjct: 607 IAQALEANGIEANPKDYLTFFCLGNREVKQEGEYEPEEHPEPDTDYIRAQEARRFMIYVH 666

Query: 671 SKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWY 730
           +K+MIVDD Y++IGSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR+SLWY
Sbjct: 667 TKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPYHLATRQPARGQIHGFRMSLWY 726

Query: 731 EHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSIE 762
           EH    E+VF  PE+++CV++V  +A++ W +YS +++      HL++YP+ V  DGS+ 
Sbjct: 727 EHLGMLEDVFQRPESVECVQKVNEVAEKYWDLYSSDDLEQDLPGHLLSYPIGVTADGSVT 786

BLAST of CmaCh01G000680 vs. Swiss-Prot
Match: PLDA1_ORYSJ (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica GN=PLD1 PE=1 SV=2)

HSP 1 Score: 623.2 bits (1606), Expect = 3.7e-177
Identity = 334/811 (41.18%), Postives = 475/811 (58.57%), Query Frame = 1

Query: 11  HGTLEVTVFHATTYTP------SSPLDC------------IFSGGKQSYVTIKINNIEVA 70
           HGTL  T+F A + +       S+P               +  G  + Y TI +    V 
Sbjct: 7   HGTLHATIFEAASLSNPHRASGSAPKFIRKFVEGIEDTVGVGKGATKVYSTIDLEKARVG 66

Query: 71  RT----NHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV----LGRFQIQAQQILKESS 130
           RT    N   +  W ++F + CAH + S +  T++    +    +GR  +  Q++L    
Sbjct: 67  RTRMITNEPINPRWYESFHIYCAH-MASNVIFTVKIDNPIGATNIGRAYLPVQELLNGEE 126

Query: 131 FINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCH 190
            I+ +  +   N +P  E ++   L +     + +W + + + +Y G+    F  R  C 
Sbjct: 127 -IDRWLDICDNNREPVGESKIHVKLQYFDVSKDRNWARGVRSTKYPGVPYTFFSQRQGCK 186

Query: 191 VTLYQDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVR 250
           VTLYQDAH    F P    +      P R WED++ AI NA+HL+YI GWS   ++ LVR
Sbjct: 187 VTLYQDAHVPDNFIPKIPLADGKNYEPHRCWEDIFDAISNAQHLIYITGWSVYTEITLVR 246

Query: 251 DPQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFS 310
           D     P    V LGELLK+KA EGV V +++WDD TS+ ++K  G+M THDE    YF 
Sbjct: 247 DSNRPKPGG-DVTLGELLKKKASEGVRVLMLVWDDRTSVGLLKRDGLMATHDEETENYFH 306

Query: 311 NSKVVCRLCPKLHPMSPPI---------FSHHQKTITVDAQT-HINARNREIMSFIGGLD 370
            S V C LCP+    S  I         F+HHQK + VD +  +  ++ R I+SF+GGLD
Sbjct: 307 GSDVNCVLCPRNPDDSGSIVQDLSISTMFTHHQKIVVVDHELPNQGSQQRRIVSFVGGLD 366

Query: 371 LCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDI 430
           LCDGRYDT+ HSLF TL++  H  DF+Q + + A ++KGGPREPWHD+H+R+ G  AWD+
Sbjct: 367 LCDGRYDTQYHSLFRTLDSTHHD-DFHQPNFATASIKKGGPREPWHDIHSRLEGPIAWDV 426

Query: 431 LTNFEQRWTKQSDASLLVPTSILLKLMPPLESNTNPQ--KDWNVQVFRSIDHLSASQV-- 490
           L NFEQRW KQ    LL+    L   + P      P+  + WNVQ+FRSID  +A     
Sbjct: 427 LYNFEQRWRKQGGKDLLLQLRDLSDTIIPPSPVMFPEDRETWNVQLFRSIDGGAAFGFPD 486

Query: 491 ------------FRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWD----KDQH 550
                        ++  ++R+I +AY+ AIRRA+ FIYIENQYF+G  + W     K + 
Sbjct: 487 TPEEAAKAGLVSGKDQIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYAWKPEGIKPED 546

Query: 551 CGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYR 610
            G  +LIP E+ALKV +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY 
Sbjct: 547 IGALHLIPKELALKVVSKIEAGERFTVYVVVPMWPEGVPESGSVQAILDWQRRTMEMMYT 606

Query: 611 LIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRRRFMVYV 670
            I EA+Q  G +A+P+DYL FFCL NRE ++  ++ P   P+  T+Y  AQ  RRFM+YV
Sbjct: 607 DITEALQAKGIEANPKDYLTFFCLGNREVKQAGEYQPEEQPEADTDYSRAQEARRFMIYV 666

Query: 671 HSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLW 730
           H+K+MIVDD Y++IGSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR++LW
Sbjct: 667 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLATRQPARGQIHGFRMALW 726

Query: 731 YEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKVKQDGSI 762
           YEH    ++VF  PE+L+CV++V  IA++ W +YS +++      HL++YP+ V  DG +
Sbjct: 727 YEHLGMLDDVFQRPESLECVQKVNRIAEKYWDMYSSDDLQQDLPGHLLSYPIGVASDGVV 786

BLAST of CmaCh01G000680 vs. Swiss-Prot
Match: PLDA1_TOBAC (Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PLD1 PE=2 SV=2)

HSP 1 Score: 608.6 bits (1568), Expect = 9.3e-173
Identity = 326/808 (40.35%), Postives = 469/808 (58.04%), Query Frame = 1

Query: 11  HGTLEVTVFHATTYTPSSP----------LDCIFSGGKQS---YVTIKINNIEVART--- 70
           HGTL VT++                    ++     GK +   Y T+ +    V RT   
Sbjct: 7   HGTLHVTIYEVDNLQKEGGGHFFSKIKEHVEETIGFGKGTPAIYATVDLEKARVGRTRKI 66

Query: 71  -NHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV----LGRFQIQAQQILKESSFINGF 130
            N   +  W ++F + CAH + S +  T++    +    +GR  +  +++L E   I+ +
Sbjct: 67  KNEPNNPRWYESFHIYCAH-MASNVIFTVKDDNPIGATLIGRAYVPVEELL-EGEEIDKW 126

Query: 131 FPLLMENGKPSPE-LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLY 190
             +L     P  E  ++   L F     + +W++ + + +Y G+    F  R+ C V+LY
Sbjct: 127 VEILDREMNPIAEGSKIHVKLQFFDVSRDPNWERGIRSSKYPGVPYTFFAQRTGCRVSLY 186

Query: 191 QDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT 250
           QDAH    F P  P  G     P R WED++ AI NAKHL+YI GWS   ++ LVRD + 
Sbjct: 187 QDAHVPDNFIPKIPLSGGKYYEPHRCWEDIFDAIINAKHLIYITGWSVYTEITLVRDSRR 246

Query: 251 DIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKV 310
             P    + LGELLK+KA EGV V +++WDD TS+ ++K  G+M THD+    +F  ++V
Sbjct: 247 QKPGG-DITLGELLKKKASEGVKVLMLVWDDRTSVGLLKKDGLMATHDQETEQFFQGTEV 306

Query: 311 VCRLCPKLHPMSPPI---------FSHHQKTITVDAQTHIN-ARNREIMSFIGGLDLCDG 370
            C LCP+       I         F+HHQK + VD++     +  R I+SF+GG+DLCDG
Sbjct: 307 NCVLCPRNPDDGGSIVQSLQIGTMFTHHQKIVVVDSELPSGESEKRRILSFVGGIDLCDG 366

Query: 371 RYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNF 430
           RYDT  HSLF TL+T  H  DF+Q +     + KGGPREPWHD+H+R+ G  AWD+L NF
Sbjct: 367 RYDTPFHSLFRTLDTAHHD-DFHQPNFPDGAITKGGPREPWHDIHSRLEGPIAWDVLFNF 426

Query: 431 EQRWTKQSDASLLVPTSIL--LKLMPPLESNTNPQKDWNVQVFRSIDHLSASQVFR---- 490
           EQRW KQ    +LV    L  + + P    + +  + WNVQ+FRSID  +A         
Sbjct: 427 EQRWRKQGGKDVLVNFRELDDIIIPPSPVMHLDDSETWNVQLFRSIDEGAAFGFPETPED 486

Query: 491 ----------NLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKD----QHCGCT 550
                     +  ++R+I +AY+ AIRRA+ FIYIENQYF+G  + W  D    +  G  
Sbjct: 487 AAKAGLVSGXDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSYDWQSDDIKVEDIGAL 546

Query: 551 NLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGE 610
           ++IP E+ALK+ +KI+A E+F VYVV+PMWPEG PES SV+ +L W R+TM MMY+ I +
Sbjct: 547 HVIPKELALKIVSKIEAGERFTVYVVVPMWPEGIPESASVQAILDWQRRTMEMMYKHIVQ 606

Query: 611 AIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRRRFMVYVHSKV 670
           A+   G +  PR+YL FFC+ NRE ++   + P  +P+  ++Y  AQ  RRFM+YVHSK+
Sbjct: 607 ALNAKGIEEDPRNYLTFFCIGNREVKKSGAYEPSETPEPDSDYIRAQEARRFMIYVHSKM 666

Query: 671 MIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHT 730
           MIVDD Y+++GSAN+NQRSMDG RD+EIA+G YQ  +     P    I  FR++LWYEH 
Sbjct: 667 MIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPHHLATREPARGQIHGFRMALWYEHL 726

Query: 731 QRFEEVFLNPENLQCVERVRSIADESWKIYSGEEV-ADMKGVHLVTYPVKVKQDGSIEDL 762
              +E FL+PE+ +CV +V  +AD+ W +YS E +  D+ G HL+ YP+ V  +G + +L
Sbjct: 727 GMLDETFLHPESEECVSKVNRMADKYWDLYSSESLERDLPG-HLLRYPIGVASEGDVTEL 786

BLAST of CmaCh01G000680 vs. Swiss-Prot
Match: PLDA1_CYNCA (Phospholipase D alpha 1 OS=Cynara cardunculus GN=PLD1 PE=1 SV=2)

HSP 1 Score: 607.4 bits (1565), Expect = 2.1e-172
Identity = 326/808 (40.35%), Postives = 465/808 (57.55%), Query Frame = 1

Query: 11  HGTLEVTVFHATTYTPSSPLDCIFS-------------GGKQSYVTIKINNIEVART--- 70
           HGTL VTV+           +                 G  + Y TI +    V RT   
Sbjct: 7   HGTLHVTVYEVDKLREGGGPNVFGKLMANIQETVGFGEGTPKIYATIDLEKSRVGRTRMI 66

Query: 71  -NHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV----LGRFQIQAQQILKESSFINGF 130
            N   +  W ++F + CAH   S I  T++    +    LGR  +  +++L +   ++ +
Sbjct: 67  ENEPQNPRWYESFHIYCAHH-ASNIIFTVKDDNPIGATLLGRAYMPVRELL-DGDEVDKW 126

Query: 131 FPLLMENGKPSPE-LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLY 190
             ++ E+  P+P   ++   L +     + +W + ++ G+Y G+    F  R  C V+LY
Sbjct: 127 IEIMDEDNNPTPAGSKIHVKLQYFDVTQDRNWDRGIKTGKYPGVPYTFFAQRQGCRVSLY 186

Query: 191 QDAHHVSTFQPPFHGSSA----PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQT 250
           QDAH    F P    +      P R WED++ AI +AKH +YI GWS   ++ L+RDP  
Sbjct: 187 QDAHVPDNFIPKISLAGGKYYEPHRCWEDIFDAISDAKHFIYITGWSVYTQIPLIRDPNR 246

Query: 251 DIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKV 310
             P    V LG+LLK+KA+EGV V +++WDD TS+ V K  G+M THDE    +F ++ V
Sbjct: 247 QKPGG-DVLLGQLLKKKADEGVRVAMLVWDDRTSVNVFKEDGLMATHDEETENFFKDTDV 306

Query: 311 VCRLCPKLHPMSPPI---------FSHHQKTITVDAQT-HINARNREIMSFIGGLDLCDG 370
            C LCP+       I         F+HHQK + VD +     ++ R +MSF+GG+DLCDG
Sbjct: 307 HCILCPRDPDDGGSIIQDLKVSTMFTHHQKIVVVDHELPRGGSQKRRVMSFVGGIDLCDG 366

Query: 371 RYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNF 430
           RYD+  H LF TL++  H  DF+Q + +GA + KGGPREPWHD+H+RV G  AWD+L NF
Sbjct: 367 RYDSAFHPLFSTLDSAHHD-DFHQPNYAGASIAKGGPREPWHDIHSRVEGPIAWDVLFNF 426

Query: 431 EQRWTKQSDASLLVPTSILLKLMPPLESNT--NPQKDWNVQVFRSID------------H 490
           EQRW KQ   ++LV    L  ++ P    T  N Q+ WNVQ+FRSID             
Sbjct: 427 EQRWRKQGGKNVLVDLKQLDDILIPPSPVTFPNDQETWNVQLFRSIDGGAAFGFPDTPEE 486

Query: 491 LSASQVF--RNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLWDKDQ----HCGCT 550
            S S +   ++  ++R+I +AY+ AIRRA+ FIYIENQYF+G    W  D       G  
Sbjct: 487 ASKSGLVSGKDNIIDRSIQDAYINAIRRAKHFIYIENQYFLGSSFAWKSDDIDVDEVGAL 546

Query: 551 NLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQTMTMMYRLIGE 610
           +LIP E++LK+  KI+  EKF VY+V+PMWPEG PE+ SV+ +L W R+TM MMY+ I +
Sbjct: 547 HLIPKELSLKIVTKIQEGEKFIVYIVVPMWPEGIPENGSVQAILDWQRRTMEMMYKDIVD 606

Query: 611 AIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRRRFMVYVHSKV 670
           A+Q+ G    PR+YL FFCL NRE ++  ++ P  +P+  + Y  AQ  RRFM+YVHSK+
Sbjct: 607 ALQDKGLDDDPREYLTFFCLGNREAKKSGEYEPTEAPEPDSGYLHAQENRRFMIYVHSKM 666

Query: 671 MIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDISTFRLSLWYEHT 730
           MIVDD Y+++GSAN+NQRSMDG RD+EIA+G YQ  +   + P    +  FR++LWYEH 
Sbjct: 667 MIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPYHLATQTPARGHVHGFRMALWYEHL 726

Query: 731 QRFEEVFLNPENLQCVERVRSIADESWKIYSGEEV-ADMKGVHLVTYPVKVKQDGSIEDL 762
              ++ F  PEN  CV +   +AD+ W +Y+ E++  D+ G HL+ YPV V + G I +L
Sbjct: 727 GMLDDSFERPENKDCVNKANEMADKCWDLYASEDLDRDLPG-HLLRYPVGVTRKGDITEL 786

BLAST of CmaCh01G000680 vs. TrEMBL
Match: A0A0A0LGB5_CUCSA (Phospholipase D OS=Cucumis sativus GN=Csa_3G848850 PE=3 SV=1)

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 668/761 (87.78%), Postives = 715/761 (93.96%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  FFHGTLE                C+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 1   MAMEVKQKFFHGTLE----------------CLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLP+IKNAGIM THDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q  RRFM+YVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 661 DISKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 745

BLAST of CmaCh01G000680 vs. TrEMBL
Match: B9MWR1_POPTR (Phospholipase D OS=Populus trichocarpa GN=POPTR_0003s02990g PE=3 SV=1)

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 525/760 (69.08%), Postives = 636/760 (83.68%), Query Frame = 1

Query: 3   VQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 62
           ++G+  F HGTLE T+F AT YTP  P +CIF  G  +YVTIKI N +VA+T+HE DRVW
Sbjct: 1   MEGKQKFLHGTLEATIFDATPYTPPFPFNCIFMNGSPTYVTIKIGNKKVAKTSHECDRVW 60

Query: 63  NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 122
           NQTF +LCAH L STITITM+T  S+LG+F IQA +I+ E+S INGFFPL+MENGKP+P+
Sbjct: 61  NQTFHILCAHSLDSTITITMKTKCSILGKFHIQAHKIVTEASLINGFFPLVMENGKPNPQ 120

Query: 123 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFH 182
           L+LRFMLWF+PA  E +W K+L+NGE++GLRN TFP RSNCHVTLYQDAHH S+F+PPF 
Sbjct: 121 LKLRFMLWFKPAELETTWAKILDNGEFQGLRNATFPQRSNCHVTLYQDAHHCSSFKPPFD 180

Query: 183 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 242
              +P +LWEDVYKA++ AKHL+YIAGWSFNPKMVLVRDP+TD+P+A GV LGELLK+K 
Sbjct: 181 LCGSPTKLWEDVYKAVEGAKHLIYIAGWSFNPKMVLVRDPETDMPHARGVMLGELLKRKG 240

Query: 243 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSH 302
           EEGVAVR+M+WDDETSLP IKN G+M  HDE+A AYF ++KV+C+LCP+LH   P +F+H
Sbjct: 241 EEGVAVRVMLWDDETSLPFIKNKGVMGVHDEDAFAYFKHTKVICKLCPRLHHKFPTLFAH 300

Query: 303 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 362
           HQKTITVDA+   +   REIMSF+GGLDLCDGRYDTE+HSLFHTLNTESHC DFYQT+I+
Sbjct: 301 HQKTITVDARARDSISEREIMSFVGGLDLCDGRYDTERHSLFHTLNTESHCFDFYQTNIA 360

Query: 363 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM-PPLE 422
           GA L+KGGPREPWHD HA + G+AA D+LTNFEQRW KQ D S+LVP S +  LM  P  
Sbjct: 361 GASLRKGGPREPWHDAHACIVGQAALDVLTNFEQRWNKQCDGSVLVPISSIPNLMHQPFP 420

Query: 423 SNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGG 482
           S+ +  ++W VQVFRSIDH+SA  + RNL VER+IHEAYVEAIRRAERFIYIENQYFIGG
Sbjct: 421 SSVSNDRNWKVQVFRSIDHVSAIHLARNLRVERSIHEAYVEAIRRAERFIYIENQYFIGG 480

Query: 483 CHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWT 542
           C LWD+D+HCGCTNLIPIEIALKV NKI+A+E+FAVY+++PMWPEG P+SE V+D+LHWT
Sbjct: 481 CQLWDEDRHCGCTNLIPIEIALKVVNKIRAKERFAVYILMPMWPEGVPDSEPVQDILHWT 540

Query: 543 RQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQ 602
           RQTM MMY+L+GEA+QE+GE  HPRDYLNFFCLANREEE K + VPP+SP  +T+YW+AQ
Sbjct: 541 RQTMAMMYKLVGEALQESGEPGHPRDYLNFFCLANREEENKGELVPPYSPHPSTQYWNAQ 600

Query: 603 HRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRD 662
             RRFMVYVHSK+MIVDD Y+LIGSANVNQRSMDG RDTEIAIGCYQ +N G+   N RD
Sbjct: 601 KHRRFMVYVHSKLMIVDDAYMLIGSANVNQRSMDGRRDTEIAIGCYQPKN-GENTRNPRD 660

Query: 663 ISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYP 722
           I  +R+SLWYEHT   EE+FL PE+L C +R+R + ++ W +Y+GEEV DM+GVHLV YP
Sbjct: 661 ILAYRMSLWYEHTGLDEEIFLEPESLACAQRMRLVGEQMWNVYAGEEVVDMEGVHLVNYP 720

Query: 723 VKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           ++V +DG++EDL + GG+FPDTK P+KGRRS  LPPIFTT
Sbjct: 721 LRVTKDGAVEDLVDGGGNFPDTKSPVKGRRSNMLPPIFTT 759

BLAST of CmaCh01G000680 vs. TrEMBL
Match: B9SN47_RICCO (Phospholipase D OS=Ricinus communis GN=RCOM_0312720 PE=3 SV=1)

HSP 1 Score: 1151.7 bits (2978), Expect = 0.0e+00
Identity = 523/757 (69.09%), Postives = 634/757 (83.75%), Query Frame = 1

Query: 9   FFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVWNQTFRV 68
           F HGT+EVT+F AT Y  + P +C+F  GK +YVTIKI N +VA+T+ E+DRVWNQTF++
Sbjct: 7   FLHGTVEVTIFDATPYISTFPFNCLFLNGKPTYVTIKIGNKKVAKTSQEHDRVWNQTFQI 66

Query: 69  LCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRLRFM 128
           LCAHPL S ITITM+T  S+LG+F IQA +I+KE++FINGFFPL+ME GKP+PEL+LRFM
Sbjct: 67  LCAHPLDSIITITMKTKCSILGKFHIQADKIVKEATFINGFFPLIMETGKPNPELKLRFM 126

Query: 129 LWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFHGSSAPR 188
           LWF+PA  E +W+K++ NG+ +GLRN TFP RSNCHVTLYQDAHH++TFQPP     +PR
Sbjct: 127 LWFKPAQLEPTWRKIISNGQSQGLRNATFPQRSNCHVTLYQDAHHLNTFQPPLALCGSPR 186

Query: 189 RLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKAEEGVAV 248
           +LWEDVYKAID AK L+YIAGWSFNPKMVLVRD +TDIP+A G+KLG+LLK+KAEEGVAV
Sbjct: 187 KLWEDVYKAIDGAKLLIYIAGWSFNPKMVLVRDSETDIPHARGLKLGDLLKRKAEEGVAV 246

Query: 249 RLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSHHQKTIT 308
           R+MIWDDETSLP+IKN G+M THDE+A AYF ++KV+C+LCP+LH   P  F+HHQKTIT
Sbjct: 247 RIMIWDDETSLPIIKNKGVMGTHDEDAFAYFKHTKVICKLCPRLHNKFPTFFAHHQKTIT 306

Query: 309 VDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQK 368
           VD + + +  +REIMSFIGGLDLCDGR+DTEQHSLF TLN ESHC DFYQT+I+GA LQK
Sbjct: 307 VDTRANDSINDREIMSFIGGLDLCDGRFDTEQHSLFQTLNAESHCGDFYQTNIAGANLQK 366

Query: 369 GGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKL----MPPLESNT 428
           GGPREPWHD HA + GEAAWD+L NFEQRWTKQ D SLL+ TS +  L      P+ S+ 
Sbjct: 367 GGPREPWHDAHACIVGEAAWDVLANFEQRWTKQCDPSLLISTSSISNLRHQPYNPVSSSI 426

Query: 429 NPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHL 488
           +  ++W VQV+RSIDH+SAS++ RNLT ER+IHEAYVEAIRRAERFIYIENQYFIGGCHL
Sbjct: 427 SNGRNWKVQVYRSIDHVSASKMARNLTSERSIHEAYVEAIRRAERFIYIENQYFIGGCHL 486

Query: 489 WDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQT 548
           WDKD+HCGC NLIPIEIALK+ +KIKA+E+FAVY+++PMWPEG PESE V+D+LHWTR+T
Sbjct: 487 WDKDKHCGCRNLIPIEIALKIVSKIKAKERFAVYILMPMWPEGVPESEPVQDILHWTRET 546

Query: 549 MTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHRR 608
           M MMY+LIGEA+QE+GE  HPRDYLNFFCLANREE  K +FVPP+SP   T+YW+AQ  R
Sbjct: 547 MAMMYKLIGEALQESGEPGHPRDYLNFFCLANREEASKGEFVPPYSPHQGTQYWNAQKNR 606

Query: 609 RFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRDIST 668
           RFMVYVHSK+MIVDD Y+LIGSANVNQRSMDG+RDTEIAIGCYQ EN G E  + RDI  
Sbjct: 607 RFMVYVHSKLMIVDDAYILIGSANVNQRSMDGKRDTEIAIGCYQQEN-GTEKASPRDIQA 666

Query: 669 FRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYPVKV 728
           +R+SLWYEHT   EE  L P++L+CV ++ S+ +  WKIYSGEEV DM+GVHLV YP+ V
Sbjct: 667 YRMSLWYEHTGLVEETLLQPQSLECVGKIYSLGEAMWKIYSGEEVTDMEGVHLVNYPINV 726

Query: 729 KQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
            +DG +ED+++  G+FPDTK P+KGRRS  LP +FTT
Sbjct: 727 TKDGLVEDIDDGNGNFPDTKTPVKGRRSKVLPCVFTT 762

BLAST of CmaCh01G000680 vs. TrEMBL
Match: M5XB63_PRUPE (Phospholipase D OS=Prunus persica GN=PRUPE_ppa022510mg PE=3 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 537/761 (70.57%), Postives = 637/761 (83.71%), Query Frame = 1

Query: 7   HTFFHGTLEVTVFHATTYTPSS-PLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVWNQT 66
           H F HGTLE T+FHAT YTP   P +CIF  GK + VTIKI+N +VART HE DRVWNQT
Sbjct: 6   HDFLHGTLEATIFHATPYTPPPFPFNCIFGAGKPACVTIKIDNKKVARTTHERDRVWNQT 65

Query: 67  FRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRL 126
           FR+LCA+P  STITITM+T  S+LG+FQ+QA +IL E+SF+N F PL++ENGKP+PEL+L
Sbjct: 66  FRILCAYPSDSTITITMKTKCSILGKFQMQAHEILNEASFVNSFLPLVIENGKPNPELKL 125

Query: 127 RFMLWFQPAVYELSWKKVL-ENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFHGS 186
           RFMLWF+PA +E +W ++  +N +++GLRN TFP RSN HVTLYQDAHH STF+P     
Sbjct: 126 RFMLWFKPAQFEPTWGQMTADNVQFQGLRNATFPQRSNSHVTLYQDAHHCSTFKPSSELC 185

Query: 187 SAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKAEE 246
             PRRLWEDVYKA++ AK+L+YIAGWSFNPKMVLVRDPQTDIP+A GVKLGELLKQKAEE
Sbjct: 186 GTPRRLWEDVYKAMEGAKNLIYIAGWSFNPKMVLVRDPQTDIPHARGVKLGELLKQKAEE 245

Query: 247 GVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSHHQ 306
           GVAVR+M+WDDETSLP+IKN GIM+THDE+A  YF+++KV+CRLCP+LH   P IFSHHQ
Sbjct: 246 GVAVRIMLWDDETSLPIIKNKGIMRTHDEDAFYYFAHTKVICRLCPRLHNKFPTIFSHHQ 305

Query: 307 KTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGA 366
           KTITVD ++  +A +REIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQT+ISGA
Sbjct: 306 KTITVDTKSSTSASDREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCSDFYQTNISGA 365

Query: 367 KLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM-PPLESN 426
            LQKGGPR PWHD HA VTGEAAWD+LTNFEQRW KQ D S+LVP+S L  L+     S 
Sbjct: 366 SLQKGGPRTPWHDAHACVTGEAAWDVLTNFEQRWAKQCDPSVLVPSSTLTSLIQQTYASK 425

Query: 427 TNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCH 486
              ++ WNVQV RSIDH+SASQ+FRNLTVE++IHEAYVEAIR A++F+YIENQYFIGGC 
Sbjct: 426 PTSERGWNVQVLRSIDHVSASQLFRNLTVEQSIHEAYVEAIRHADKFLYIENQYFIGGCR 485

Query: 487 LWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTRQ 546
           LW+KDQ+CGC NLIPIEIALKV NKIKA+E+FAVY+VIPMWPEG PESESV+D+LHWTR+
Sbjct: 486 LWEKDQNCGCRNLIPIEIALKVVNKIKAKERFAVYIVIPMWPEGLPESESVQDILHWTRE 545

Query: 547 TMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQHR 606
           TM+MMYRLIGEAI+E+G + HPRDYLNF+CLANRE E + DF+PPHSP  AT+Y +AQ  
Sbjct: 546 TMSMMYRLIGEAIKESGVEGHPRDYLNFYCLANREVESQGDFIPPHSPNSATQYGNAQKH 605

Query: 607 RRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLEN--DGKELPNGRD 666
           RRFMVYVHSK+MIVDD YL+IGSAN+NQRSMDG RDTEIAIGCYQ +N  D   +  G D
Sbjct: 606 RRFMVYVHSKLMIVDDAYLIIGSANINQRSMDGHRDTEIAIGCYQSKNGDDKAMMSAGGD 665

Query: 667 ISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYP 726
           I  +R+SLWYEH  R EE+F  PE+L+CV+ VR I D+ WK YSGEEV DM+GVHLVTYP
Sbjct: 666 IEAYRMSLWYEHIGRAEELFKQPESLECVQTVRLIGDQMWKTYSGEEVVDMEGVHLVTYP 725

Query: 727 VKVKQDGSIEDL-EENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           + V ++G +EDL E +GG FPDTK  +KG+RS  +PP+FTT
Sbjct: 726 MNVTEEGHVEDLVEGSGGLFPDTKSAVKGKRSKVIPPMFTT 766

BLAST of CmaCh01G000680 vs. TrEMBL
Match: A0A061GBY3_THECC (Phospholipase D OS=Theobroma cacao GN=TCM_029016 PE=3 SV=1)

HSP 1 Score: 1146.7 bits (2965), Expect = 0.0e+00
Identity = 530/765 (69.28%), Postives = 643/765 (84.05%), Query Frame = 1

Query: 3   VQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 62
           ++G+  F HGTLE T+F AT Y P  PL+C+ + GK +YVTIK++N +VA+T  E DRVW
Sbjct: 1   MEGKQKFLHGTLEATIFDATPYIPPFPLNCVLANGKPTYVTIKLDNKKVAKTTLERDRVW 60

Query: 63  NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 122
           NQTF++LCAHP  STITITM+T  S+LG+F IQA QIL E+S I+GFFPL MENGK +PE
Sbjct: 61  NQTFQILCAHPPDSTITITMKTKCSILGKFCIQAGQILNEASLISGFFPLQMENGKTNPE 120

Query: 123 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFH 182
           L+LRFMLWF+PA +E +W +VL+ G ++GLRN TFP RSNCHV LYQDAHH S FQPPF 
Sbjct: 121 LKLRFMLWFKPAEFEPTWGEVLKYGGFQGLRNATFPQRSNCHVMLYQDAHHSSAFQPPFS 180

Query: 183 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 242
              +PR+LWEDVYKAI+ AKHL+YIAGWSFNPKMVLVRDPQT+IP+A G+KLGELLK+KA
Sbjct: 181 LCGSPRKLWEDVYKAIEGAKHLIYIAGWSFNPKMVLVRDPQTNIPHAKGIKLGELLKRKA 240

Query: 243 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSH 302
           EEGVAVR+M+W+DETSLP IKN G+M+THDE+A AYF ++KV C+LCP+LH   P +F+H
Sbjct: 241 EEGVAVRVMLWNDETSLPFIKNKGVMRTHDEDAFAYFKHTKVRCKLCPRLHHKFPTLFAH 300

Query: 303 HQKTITVDAQTH--INARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 362
           HQKTITVDA+T+   +  +REIMSF+GG+DLCDGRYDTEQHSLF TLNT +HC DFYQT+
Sbjct: 301 HQKTITVDARTYSTSSVNDREIMSFVGGVDLCDGRYDTEQHSLFRTLNTGAHCFDFYQTN 360

Query: 363 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM-PP 422
           ISGA L KGGPREPWHD HA +TGEAAWD+L NFEQRWTKQ D SLL+PTS +  L+  P
Sbjct: 361 ISGASLHKGGPREPWHDAHACITGEAAWDVLANFEQRWTKQCDPSLLLPTSSIPNLIRQP 420

Query: 423 LESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFI 482
             S+ +  ++W VQVFRSIDH+S SQ+ +NLTVE++IHEAYVEAIRRAERFIYIENQYFI
Sbjct: 421 FASSISNDRNWKVQVFRSIDHVSVSQLSKNLTVEQSIHEAYVEAIRRAERFIYIENQYFI 480

Query: 483 GGCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLH 542
           GGCHLWDKD+H GC NLIP+EIALKVA+KIKA+E+F VY++IPMWPEG P+SE V+D+LH
Sbjct: 481 GGCHLWDKDKHSGCRNLIPVEIALKVASKIKAKERFTVYILIPMWPEGVPDSEPVQDILH 540

Query: 543 WTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWS 602
           WTR+TM++MY+LIGE+I+E+GE  HPRDYLNFFCLANRE + K +FVPPHSPQ +T+YW+
Sbjct: 541 WTRETMSIMYKLIGESIKESGEPGHPRDYLNFFCLANRETQSKEEFVPPHSPQPSTQYWN 600

Query: 603 AQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQL-EN-DGKELP 662
           AQ  RRFMVYVHSKVMIVDDLY+LIGSANVNQRSMDG RDTEIAIGCYQL EN +     
Sbjct: 601 AQKHRRFMVYVHSKVMIVDDLYILIGSANVNQRSMDGRRDTEIAIGCYQLPENPESNNST 660

Query: 663 NGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSG-EEVADMKGVH 722
             RDI  +R+SLWYEHT   ++VFL P++L+CV++VRS+ D+ W+IY+  EE+ADM+GVH
Sbjct: 661 TPRDIHAYRMSLWYEHTGLADDVFLEPQSLECVQKVRSLGDQMWQIYANEEEIADMEGVH 720

Query: 723 LVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           LVTYPV V  DG  ED+ + GG+FPDTK P+KGRRS  LPPIFTT
Sbjct: 721 LVTYPVNVTVDGLTEDVADGGGNFPDTKSPVKGRRSKMLPPIFTT 765

BLAST of CmaCh01G000680 vs. TAIR10
Match: AT1G55180.1 (AT1G55180.1 phospholipase D alpha 4)

HSP 1 Score: 976.1 bits (2522), Expect = 1.3e-284
Identity = 466/779 (59.82%), Postives = 597/779 (76.64%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  +FHGTLE+T+F AT ++P  P +CI +  K +YVTIKIN  +VA+T+ EYDR
Sbjct: 1   MELEEQKKYFHGTLEITIFDATPFSPPFPFNCICTKPKAAYVTIKINKKKVAKTSSEYDR 60

Query: 61  VWNQTFRVLCAHPLT-STITITMRTTRSVLGRFQIQAQQIL-KESSFINGFFPLLMENGK 120
           +WNQTF++LCAHP+T +TITIT++T  SVLGRF+I A+QIL   S+ INGFFPL+ +NG 
Sbjct: 61  IWNQTFQILCAHPVTDTTITITLKTRCSVLGRFRISAEQILTSNSAVINGFFPLIADNGS 120

Query: 121 PSPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQ 180
               L+L+ ++WF+PA  E  W + LE   ++G+RN +FP RSNC V LYQDAHH +TF 
Sbjct: 121 TKRNLKLKCLMWFRPAYLEPGWCRALEEASFQGIRNASFPQRSNCRVVLYQDAHHKATFD 180

Query: 181 P-----PFHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVK 240
           P     PF+     R LWEDVYKAI++A+HLVYIAGW+ NP +VLVRD +T+IP+A+GV 
Sbjct: 181 PRVDDVPFNA----RNLWEDVYKAIESARHLVYIAGWALNPNLVLVRDNETEIPHAVGVT 240

Query: 241 LGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLH 300
           +GELLK+K+EEGVAVR+M+W+DETSLP+IKN G+M+T+ E A AYF N+ VVCRLCP+LH
Sbjct: 241 VGELLKRKSEEGVAVRVMLWNDETSLPMIKNKGVMRTNVERALAYFRNTNVVCRLCPRLH 300

Query: 301 PMSPPIFSHHQKTITVDAQ-THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESH 360
              P  F+HHQKTIT+D + T+ + + REIMSF+GG DLCDGRYDTE+HSLF TL TE+ 
Sbjct: 301 KKLPTAFAHHQKTITLDTRVTNSSTKEREIMSFLGGFDLCDGRYDTEEHSLFRTLGTEA- 360

Query: 361 CCDFYQTSISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTS- 420
             DFYQTS++GAKL +GGPREPWHD H  V G AAWD+L NFEQRWTKQ + S+LV TS 
Sbjct: 361 --DFYQTSVAGAKLSRGGPREPWHDCHVSVVGGAAWDVLKNFEQRWTKQCNPSVLVNTSG 420

Query: 421 --ILLKLMPPLESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAER 480
              L+ L  P E N    + WNVQV RSIDH+SA+++ R L VE+++H+ YV AIR+AER
Sbjct: 421 IRNLVNLTGPTEENN---RKWNVQVLRSIDHISATEMPRGLPVEKSVHDGYVAAIRKAER 480

Query: 481 FIYIENQYFIGGCHLWDK--DQHC-GCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPE 540
           FIYIENQYF+G C  W+   D+ C GCTNLIP+EIALK+A KI+ARE+FAVY+VIPMWPE
Sbjct: 481 FIYIENQYFMGSCDHWESKNDKICSGCTNLIPVEIALKIAAKIRARERFAVYIVIPMWPE 540

Query: 541 GPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFV 600
           GPPESE+VE++LHWTR+TM+MMY++IGEAI E G+K+HPRDYLNFFCLANREE+R  +F 
Sbjct: 541 GPPESETVEEILHWTRETMSMMYQIIGEAIWEVGDKSHPRDYLNFFCLANREEKRDGEFE 600

Query: 601 PPHSPQHATEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGC 660
              SP   T YW+AQ  RRFMVYVHSK+MIVDD Y+LIGSAN+NQRSMDG RDTEIAIGC
Sbjct: 601 AVSSPHQKTHYWNAQRNRRFMVYVHSKLMIVDDTYILIGSANINQRSMDGCRDTEIAIGC 660

Query: 661 YQLENDGKELPNGRDISTFRLSLWYEHT----QRFEEVFLNPENLQCVERVRSIADESWK 720
           YQ   +     N  +I  +RLSLWYEHT       +     PE+L+CV  +R+I ++ W+
Sbjct: 661 YQTNTN-----NTNEIQAYRLSLWYEHTGGKITADDLSSSEPESLECVRGLRTIGEQMWE 720

Query: 721 IYSGEEVADMKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           IYSG++V DM G+HLV YP+ V  DG++E++ +  G FPDTK  +KG+RS   PP+ TT
Sbjct: 721 IYSGDKVVDMLGIHLVAYPISVTGDGAVEEVGD--GCFPDTKTLVKGKRSKMFPPVLTT 762

BLAST of CmaCh01G000680 vs. TAIR10
Match: AT5G25370.1 (AT5G25370.1 phospholipase D alpha 3)

HSP 1 Score: 605.9 bits (1561), Expect = 3.4e-173
Identity = 342/826 (41.40%), Postives = 475/826 (57.51%), Query Frame = 1

Query: 6   EHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQS------------------------Y 65
           E    HGTLEV ++        S  +    G K+                         Y
Sbjct: 3   EQLLLHGTLEVKIYRIDKLHQRSRFNLCGKGNKEPTGKKTQSQIKRLTDSCTSLFGGHLY 62

Query: 66  VTIKINNIEVARTNHEYDRVWNQTFRVLCAHPLTSTITITMR----TTRSVLGRFQIQAQ 125
            TI ++   VART       W Q+F V  AH + S I  T++     + S++GR  +   
Sbjct: 63  ATIDLDRSRVARTMMRRHPKWLQSFHVYTAHSI-SKIIFTVKEDEPVSASLIGRAYLPVT 122

Query: 126 QILKESSFINGFFPLLMENGKP-SPELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVT 185
           +++     I+ +  +L EN +P     +L   + F     +++W K +    + G+ N  
Sbjct: 123 EVITGQP-IDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIILPSFNGVPNAY 182

Query: 186 FPLRSNCHVTLYQDAHHVSTFQP-PFHGSSA---PRRLWEDVYKAIDNAKHLVYIAGWSF 245
           F  R  C VTLYQDAH ++ +      G        R WE+++ AI  AKHL+YIAGWS 
Sbjct: 183 FNQREGCKVTLYQDAHVLNEYPDVTLTGGQVIYKHHRCWEEIFDAIWEAKHLIYIAGWSV 242

Query: 246 NPKMVLVRDPQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVIKNAGIMKTHD 305
           N  + LVRDP+   P    +KLGELLK+KAEE V V +++WDD TS  V K  G+M THD
Sbjct: 243 NTDVTLVRDPKRTRPGG-DLKLGELLKKKAEENVTVLMLVWDDRTSHEVFKRDGLMMTHD 302

Query: 306 ENARAYFSNSKVVCRLCPKLHPMSPPI---------FSHHQKTITVDAQTHINARNREIM 365
           +    YF N+KV C LCP+       I         F+HHQKTI VD++   +   R I+
Sbjct: 303 QETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEVDGSLTKRRIV 362

Query: 366 SFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREPWHDVHARVT 425
           SF+GG+DLCDGRYDT +H LF TLN+  H  DF+Q +  GA ++KGGPREPWHD+H ++ 
Sbjct: 363 SFLGGIDLCDGRYDTVEHPLFGTLNSV-HANDFHQPNFDGASIKKGGPREPWHDIHCKLD 422

Query: 426 GEAAWDILTNFEQRWTKQ-SDASLLVPTSILLKL-MPPLES-NTNPQKDWNVQVFRSIDH 485
           G AAWD+L NFEQRW KQ S    L+  + L ++ +PPL     + ++ W VQVFRSID 
Sbjct: 423 GPAAWDVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDNEEGWTVQVFRSIDD 482

Query: 486 LSASQVF---------------RNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGCHLW 545
             A + F               ++  +ER+I +AYV AIRRA+ FIYIENQYF+G    W
Sbjct: 483 -GAVEGFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIENQYFLGSSFGW 542

Query: 546 DKDQ----HCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWT 605
           +             LIP EI+LK+ +KI+A E+F+VY+VIP+WPEG P S SV+ +L W 
Sbjct: 543 NSRDINLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKPGSASVQAILDWQ 602

Query: 606 RQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQ 665
           R+TM MMY  I  A+++ G  A+PRDYL FFCL NRE+ +  +++PP  P+  ++Y  AQ
Sbjct: 603 RRTMEMMYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKPEANSDYARAQ 662

Query: 666 HRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQ---LENDGKELPN 725
             RRFM+YVHSK+MIVDD Y++IGSAN+NQRSMDG RDTEIA+G YQ   L +     P 
Sbjct: 663 ESRRFMIYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSHLLSTNNMRPV 722

Query: 726 GRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVA---DMKGV 762
           G+ I +FR+SLW EH +     F  PE+ +C+  V + ADE W +YS +E     D+ G 
Sbjct: 723 GQ-IFSFRISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSAQEYPRNDDLPG- 782

BLAST of CmaCh01G000680 vs. TAIR10
Match: AT3G15730.1 (AT3G15730.1 phospholipase D alpha 1)

HSP 1 Score: 605.1 bits (1559), Expect = 5.8e-173
Identity = 323/770 (41.95%), Postives = 451/770 (58.57%), Query Frame = 1

Query: 36  GGKQSYVTIKINNIEVART----NHEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV--- 95
           G  Q Y TI +    V RT    N   +  W ++F + CAH L S I  T++    +   
Sbjct: 46  GETQLYATIDLQKARVGRTRKIKNEPKNPKWYESFHIYCAH-LASDIIFTVKDDNPIGAT 105

Query: 96  -LGRFQIQAQQILKESSFINGFFPLLMENGKP-SPELRLRFMLWFQPAVYELSWKKVLEN 155
            +GR  I   Q++     ++ +  +L  +  P     ++   L +     + +W   +++
Sbjct: 106 LIGRAYIPVDQVINGEE-VDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKS 165

Query: 156 GEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAK 215
            ++ G+    F  R  C V+LYQDAH    F P  P  G     P+R WED++ AI NAK
Sbjct: 166 AKFPGVPYTFFSQRQGCKVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAK 225

Query: 216 HLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVI 275
           HL+YI GWS   ++ LVRD +   P    V +GELLK+KA EGV V L++WDD TS+ V+
Sbjct: 226 HLIYITGWSVYAEIALVRDSRRPKPGG-DVTIGELLKKKASEGVRVLLLVWDDRTSVDVL 285

Query: 276 KNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPI---------FSHHQKTITVDAQ- 335
           K  G+M THDE    +F  S V C LCP+       I         F+HHQK + VD++ 
Sbjct: 286 KKDGLMATHDEETENFFRGSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEM 345

Query: 336 -THINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGP 395
            +   +  R I+SF+GG+DLCDGRYDT  HSLF TL+T  H  DF+Q + +GA + KGGP
Sbjct: 346 PSRGGSEMRRIVSFVGGIDLCDGRYDTPFHSLFRTLDTVHHD-DFHQPNFTGAAITKGGP 405

Query: 396 REPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM--PPLESNTNPQKD 455
           REPWHD+H+R+ G  AWD++ NFEQRW+KQ    +LV    L  ++  P           
Sbjct: 406 REPWHDIHSRLEGPIAWDVMYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDV 465

Query: 456 WNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIEN 515
           WNVQ+FRSID  +A+                 ++  ++R+I +AY+ AIRRA+ FIY+EN
Sbjct: 466 WNVQLFRSIDGGAAAGFPESPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVEN 525

Query: 516 QYFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPES 575
           QYF+G    W  D    +     +LIP E++LK+ +KI+  EKF VYVV+PMWPEG PES
Sbjct: 526 QYFLGSSFAWAADGITPEDINALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPES 585

Query: 576 ESVEDMLHWTRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSP 635
            SV+ +L W R+TM MMY+ + +A++  G +  PR+YL FFCL NRE ++  ++ P   P
Sbjct: 586 GSVQAILDWQRRTMEMMYKDVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKP 645

Query: 636 QHATEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLEN 695
              T+Y  AQ  RRFM+YVH+K+MIVDD Y++IGSAN+NQRSMDG RD+EIA+G YQ  +
Sbjct: 646 DPDTDYMRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHH 705

Query: 696 DGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVAD 755
                P    I  FR+SLWYEH    +E FL+P +L+C+E+V  I+D+ W  YS E +  
Sbjct: 706 LSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEH 765

Query: 756 MKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
               HL+ YP+ V  +G I +L      FPDTK  I G +S +LPPI TT
Sbjct: 766 DLPGHLLRYPIGVASEGDITEL-PGFEFFPDTKARILGTKSDYLPPILTT 810

BLAST of CmaCh01G000680 vs. TAIR10
Match: AT1G52570.1 (AT1G52570.1 phospholipase D alpha 2)

HSP 1 Score: 597.8 bits (1540), Expect = 9.3e-171
Identity = 322/770 (41.82%), Postives = 453/770 (58.83%), Query Frame = 1

Query: 36  GGKQSYVTIKINNIEVARTN----HEYDRVWNQTFRVLCAHPLTSTITITMRTTRSV--- 95
           G  Q Y TI +    V RT        +  W ++F + C H +   +  T++    +   
Sbjct: 46  GETQLYATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGH-MAKHVIFTVKDANPIGAT 105

Query: 96  -LGRFQIQAQQILKESSFINGFFPLLMENGKPSPELRLRFMLWFQPAVYELSWKKVLENG 155
            +GR  I  + IL           L  E    +   ++   L +     + +W + +++ 
Sbjct: 106 LIGRGYIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSA 165

Query: 156 EYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQP--PFHGSS--APRRLWEDVYKAIDNAKH 215
           ++ G+    F  R  C V+LYQDAH    F P  P  G     P R WED++ AI NAKH
Sbjct: 166 KFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKH 225

Query: 216 LVYIAGWSFNPKMVLVRDPQTDIPYALG-VKLGELLKQKAEEGVAVRLMIWDDETSLPVI 275
           L+YI GWS   ++ LVRD +   P   G V +GELLK+KA EGV V L++WDD TS+ ++
Sbjct: 226 LIYITGWSVYTEISLVRDSRR--PKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLL 285

Query: 276 KNAGIMKTHDENARAYFSNSKVVCRLCPK---------LHPMSPPIFSHHQKTITVDAQT 335
           K  G+M THDE    +F  + V C LCP+          +     +F+HHQK + VD++ 
Sbjct: 286 KKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEM 345

Query: 336 HIN-ARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPR 395
               +R+R I+SF+GGLDLCDGRYDT  HSLF TL+T  H  DF+Q + +GA + KGGPR
Sbjct: 346 PSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDTAHHD-DFHQPNFTGAAITKGGPR 405

Query: 396 EPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM-PPLESNTNPQKD-W 455
           EPWHD+H R+ G  AWD+L NFEQRW++Q    +LV    L  ++ PP     +   D W
Sbjct: 406 EPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVW 465

Query: 456 NVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIRRAERFIYIENQ 515
           NVQ+FRSID  +A+                 ++  ++R+I +AY+ AIRRA+ FIYIENQ
Sbjct: 466 NVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQ 525

Query: 516 YFIGGCHLWDKD----QHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESE 575
           YF+G    W  D    +     +LIP E++LK+ +KIKA EKF VYVV+PMWPEG PES 
Sbjct: 526 YFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESG 585

Query: 576 SVEDMLHWTRQTMTMMYRLIGEAIQETG-EKAHPRDYLNFFCLANREEERKWDFVPPHSP 635
           SV+ +L W ++TM MMY+ + +A++E G E   PRDYL FFCL NRE ++  ++ P   P
Sbjct: 586 SVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKP 645

Query: 636 QHATEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLEN 695
           +  T+Y  AQ  RRFM+YVH+K+MIVDD Y++IGSAN+NQRSMDG RD+EIA+G YQ  +
Sbjct: 646 EPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYH 705

Query: 696 DGKELPNGRDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVAD 755
                P    I  FR+SLWYEH    +E FL+P + +C+++V  +AD+ W +YS E +  
Sbjct: 706 LSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEH 765

Query: 756 MKGVHLVTYPVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
               HL+ YP+ +  +G+I +L      FPDTK  I G +S ++PPI TT
Sbjct: 766 DLPGHLLRYPIGIASEGNITEL-PGCEFFPDTKARILGVKSDYMPPILTT 810

BLAST of CmaCh01G000680 vs. TAIR10
Match: AT2G42010.1 (AT2G42010.1 phospholipase D beta 1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.5e-149
Identity = 315/769 (40.96%), Postives = 434/769 (56.44%), Query Frame = 1

Query: 41   YVTIKINNIEVART---NHEYDRVWNQTFRVLCAHPLTSTITITMRTT---RSVLGRFQI 100
            YV++ +    + RT   ++  + VW Q F V  AH       +   +      ++G   I
Sbjct: 319  YVSVSVAGAVIGRTYVMSNSENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTI 378

Query: 101  QAQQILKESSFINGFFPLLMENGKP-SPELRLRFMLWFQP----AVYELSWKKVLENGEY 160
              +QI   +  I G +P+L  NGKP  P   L   + + P    +VY      V    +Y
Sbjct: 379  PVEQIYSGAK-IEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHG---VGAGPDY 438

Query: 161  KGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPF---HGSSAPR-RLWEDVYKAIDNAKHLV 220
            +G+    FPLR    V LYQDAH      P     +G S    + W D++ AI  A+ L+
Sbjct: 439  QGVPGTYFPLRKGGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLI 498

Query: 221  YIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKAEEGVAVRLMIWDDETSLPVI--K 280
            YI GWS   K+ L+RD    +  A    LGELL+ K++EGV V L+IWDD TS  ++  K
Sbjct: 499  YITGWSVWHKVKLIRDK---LGPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYK 558

Query: 281  NAGIMKTHDENARAYFSNSKVVCRLCP----KLHPMSPP-----IFSHHQKTITVDAQTH 340
              G+M THDE  R +F +S V   LCP    K H          I++HHQK + VDA   
Sbjct: 559  TDGVMATHDEETRRFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDADAG 618

Query: 341  INARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSISGAKLQKGGPREP 400
             N   R+I++F+GGLDLCDGRYDT QH LF TL T  H  DF+  + +G     G PREP
Sbjct: 619  GN--RRKIIAFVGGLDLCDGRYDTPQHPLFRTLQT-IHKDDFHNPTFTGNL--SGCPREP 678

Query: 401  WHDVHARVTGEAAWDILTNFEQRWTKQS-------------DASLLVPT--SILLKLMPP 460
            WHD+H+++ G AA+D+LTNFE+RW K +             DA L +     IL     P
Sbjct: 679  WHDLHSKIDGPAAYDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTP 738

Query: 461  LESNTNPQKDWNVQVFRSIDHLSASQV--------------FRNLTVERTIHEAYVEAIR 520
              S  +P+  W+VQ+FRSID  S                   +N+ ++ +IH AYV+AIR
Sbjct: 739  TVSENDPEA-WHVQIFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIR 798

Query: 521  RAERFIYIENQYFIGGCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWP 580
             A+ FIYIENQYFIG  + W+  +  G  NLIP+EIALK+A KI+A E+FA Y+VIPMWP
Sbjct: 799  AAQHFIYIENQYFIGSSYNWNAHKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWP 858

Query: 581  EGPPESESVEDMLHWTRQTMTMMYRLIGEAIQETGEKA--HPRDYLNFFCLANREEERKW 640
            EG P   + + +L+W  +T+ MMY  I +A+ ETG +    P+DYLNFFCL NRE     
Sbjct: 859  EGVPTGAATQRILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGI 918

Query: 641  DFVPPHSPQHA-TEYWSAQHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEI 700
            D     SP +A T    ++  RRFMVYVHSK M+VDD Y++IGSAN+NQRSM+G RDTEI
Sbjct: 919  DNSGTGSPSNANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEI 978

Query: 701  AIGCYQLENDGKELPNG--RDISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADES 750
            A+G YQ ++      +G    I  +R+SLW EH    ++ F  PE+++CV +VR++ + +
Sbjct: 979  AMGAYQPQHTWARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERN 1038

BLAST of CmaCh01G000680 vs. NCBI nr
Match: gi|449458181|ref|XP_004146826.1| (PREDICTED: phospholipase D epsilon [Cucumis sativus])

HSP 1 Score: 1468.8 bits (3801), Expect = 0.0e+00
Identity = 682/761 (89.62%), Postives = 729/761 (95.80%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  FFHGTLEVTVFHAT Y PSSPLDC+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 1   MAMEVKQKFFHGTLEVTVFHATAYAPSSPLDCLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLP+IKNAGIM THDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q  RRFM+YVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 661 DISKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 761

BLAST of CmaCh01G000680 vs. NCBI nr
Match: gi|659093575|ref|XP_008447602.1| (PREDICTED: phospholipase D epsilon [Cucumis melo])

HSP 1 Score: 1465.7 bits (3793), Expect = 0.0e+00
Identity = 681/761 (89.49%), Postives = 729/761 (95.80%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  FFHG LEVTVFHAT YTP SPLDC+F+GGK+SYVTIKI+N EVA+T+HE+DR
Sbjct: 1   MAMEVKQKFFHGMLEVTVFHATAYTPPSPLDCLFAGGKRSYVTIKIDNKEVAQTSHEHDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCA+PLTSTITIT++T RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAYPLTSTITITLKTARSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLANGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KAEEGVAVR++IWDDETSLP+IKNAGIM THDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KAEEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQK I VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKIIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+D+HCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDRHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q  RRFM+YVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIAIGCYQ+ENDG+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAIGCYQIENDGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT+ FEEVFLNPE+L+CV+RVRSI D+SWKIYSGEEV DMKGVHLVTY
Sbjct: 661 DISRFRLSLWYEHTRGFEEVFLNPESLKCVQRVRSIGDKSWKIYSGEEVEDMKGVHLVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG++EDLEENGGHFPDTKC IKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGTMEDLEENGGHFPDTKCAIKGRRSMMLPPIFTT 761

BLAST of CmaCh01G000680 vs. NCBI nr
Match: gi|700204694|gb|KGN59827.1| (hypothetical protein Csa_3G848850 [Cucumis sativus])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 668/761 (87.78%), Postives = 715/761 (93.96%), Query Frame = 1

Query: 1   MTVQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDR 60
           M ++ +  FFHGTLE                C+F+GGK SYVTIKI+N EVA+T+HE DR
Sbjct: 1   MAMEVKQKFFHGTLE----------------CLFAGGKCSYVTIKIDNKEVAQTSHEQDR 60

Query: 61  VWNQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPS 120
           VWNQTFRVLCAHPLTST+TIT++T+RSVLG+F IQAQQILKE+SFINGFFPLLMENGKPS
Sbjct: 61  VWNQTFRVLCAHPLTSTVTITLKTSRSVLGKFYIQAQQILKEASFINGFFPLLMENGKPS 120

Query: 121 PELRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPP 180
           PEL+LRFMLWF+PAVYELSWKK+L NGEYKGLRN TFPLRSNCHVTLYQDAHH+ TFQPP
Sbjct: 121 PELKLRFMLWFKPAVYELSWKKMLGNGEYKGLRNATFPLRSNCHVTLYQDAHHLPTFQPP 180

Query: 181 FHGSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQ 240
           FHGSS PRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRD QTDIPYALGVKLGELLKQ
Sbjct: 181 FHGSSTPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDSQTDIPYALGVKLGELLKQ 240

Query: 241 KAEEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIF 300
           KA+EGVAVR++IWDDETSLP+IKNAGIM THDE+ARAYF +SKV+CRLCPKLHPMSPPIF
Sbjct: 241 KADEGVAVRILIWDDETSLPIIKNAGIMNTHDEDARAYFLHSKVICRLCPKLHPMSPPIF 300

Query: 301 SHHQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTS 360
           SHHQKTI VDAQTHINA+NREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHC DFYQTS
Sbjct: 301 SHHQKTIIVDAQTHINAQNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCRDFYQTS 360

Query: 361 ISGAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPL 420
           ISGAKLQKGGPREPWHDVHA VTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMP L
Sbjct: 361 ISGAKLQKGGPREPWHDVHACVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPQL 420

Query: 421 ESNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIG 480
           ESNTNPQKDWNVQVFRSIDHLSASQ+FRN+T+ERTIHEAYVEAIRRAERFIYIENQYFIG
Sbjct: 421 ESNTNPQKDWNVQVFRSIDHLSASQMFRNMTIERTIHEAYVEAIRRAERFIYIENQYFIG 480

Query: 481 GCHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHW 540
           GCH WD+DQHCGCTNLIPIEIALKVANKIKARE+FAVY+VIPMWPEG PESESVEDMLHW
Sbjct: 481 GCHFWDRDQHCGCTNLIPIEIALKVANKIKARERFAVYIVIPMWPEGSPESESVEDMLHW 540

Query: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSA 600
           TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDF+PPHSPQHAT+YW+A
Sbjct: 541 TRQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFIPPHSPQHATQYWNA 600

Query: 601 QHRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGR 660
           Q  RRFM+YVHSKVMIVDDLY+LIGSANVNQRSMDGERDTEIA+GCYQ+EN+G+ELPNGR
Sbjct: 601 QQHRRFMIYVHSKVMIVDDLYILIGSANVNQRSMDGERDTEIAMGCYQIENEGEELPNGR 660

Query: 661 DISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTY 720
           DIS FRLSLWYEHT  FEEVFLNPE+L+CVERVRSI D+SWKIYSGEEV DMKGVH+VTY
Sbjct: 661 DISKFRLSLWYEHTGGFEEVFLNPESLKCVERVRSIGDKSWKIYSGEEVEDMKGVHMVTY 720

Query: 721 PVKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           PVKVK+DG +EDLEENGGHFPDTKCPIKGRRSM LPPIFTT
Sbjct: 721 PVKVKEDGRMEDLEENGGHFPDTKCPIKGRRSMMLPPIFTT 745

BLAST of CmaCh01G000680 vs. NCBI nr
Match: gi|1009112032|ref|XP_015866270.1| (PREDICTED: phospholipase D alpha 4 [Ziziphus jujuba])

HSP 1 Score: 1182.5 bits (3058), Expect = 0.0e+00
Identity = 548/765 (71.63%), Postives = 644/765 (84.18%), Query Frame = 1

Query: 3   VQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 62
           ++G+H F HGTLE T+FHA+ YTPS P +CI + GK +YVTIKI+N +VA+T  E DRVW
Sbjct: 1   MEGKHKFLHGTLEATIFHASPYTPSFPFNCICANGKPAYVTIKIDNKKVAKTTQERDRVW 60

Query: 63  NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 122
           NQTF++LCAHP  STITIT++T+ SVLG+F I+A QIL E+S INGFFPL  ENGKP PE
Sbjct: 61  NQTFQILCAHPPDSTITITLKTSCSVLGKFHIEAHQILNEASLINGFFPLAPENGKPRPE 120

Query: 123 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFH 182
           L+LRF+LWF+PA  E +W K+L  G+++G RN TFP RSN HVT YQDAHH STFQPPF 
Sbjct: 121 LKLRFLLWFKPAELEPTWGKILSAGDFQGQRNATFPQRSNSHVTFYQDAHHCSTFQPPFS 180

Query: 183 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 242
               PRRLWEDVYKAI+ AKHL+YIAGWSFNPKMVLVRDPQT+IP+A GVKLGELLK+KA
Sbjct: 181 HCGTPRRLWEDVYKAIEGAKHLIYIAGWSFNPKMVLVRDPQTEIPHARGVKLGELLKRKA 240

Query: 243 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSH 302
           EEGVAVR+M+WDDETSLP+IKN GIM+THDE+A A+FS+SKVVCRLCP+LH   P +FSH
Sbjct: 241 EEGVAVRIMLWDDETSLPIIKNKGIMRTHDEDALAHFSHSKVVCRLCPRLHNKFPTLFSH 300

Query: 303 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 362
           HQKTITVD ++     +REIMSF+GG+DLCDGRYDTEQHSLFHTLNTESHC DFYQT+IS
Sbjct: 301 HQKTITVDIKSKNGLNDREIMSFVGGVDLCDGRYDTEQHSLFHTLNTESHCFDFYQTNIS 360

Query: 363 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLMPPLES 422
           GA LQKGGPREPWHD HA +TGEAAWD+LTNFEQRWTKQ D SLLVPTS L  L+    S
Sbjct: 361 GANLQKGGPREPWHDAHACITGEAAWDVLTNFEQRWTKQCDPSLLVPTSTLTNLIHQTYS 420

Query: 423 NTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGGC 482
           +   ++ W VQVFRSIDH+SASQ+F N TVE++IHEAYVEAIRRA++FIYIENQYFIGGC
Sbjct: 421 SNPSERSWKVQVFRSIDHVSASQLFGNFTVEQSIHEAYVEAIRRADKFIYIENQYFIGGC 480

Query: 483 HLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWTR 542
           HLW+ DQHCGC NLIPIEIALKV +KIKA+E+FAVY+VIPMWPEG PESESV+D+LHWTR
Sbjct: 481 HLWENDQHCGCRNLIPIEIALKVVSKIKAKERFAVYIVIPMWPEGVPESESVQDILHWTR 540

Query: 543 QTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQH 602
           +TM MMYRLIGE I+E+G+  HPRDYLNFFCLANRE E K ++VPP+SP  A+ YW AQ 
Sbjct: 541 ETMKMMYRLIGEGIKESGQAGHPRDYLNFFCLANREIESKGEYVPPYSPHPASHYWYAQK 600

Query: 603 RRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQL---ENDGKELPNG 662
            RRFMVYVHSK+MIVDD Y+LIGSAN+NQRSMDG RDTEIAIGCY+    + D  E+ +G
Sbjct: 601 HRRFMVYVHSKLMIVDDAYILIGSANINQRSMDGHRDTEIAIGCYRTRKDDEDQSEMEDG 660

Query: 663 RDISTFRLSLWYEHT--QRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHL 722
            DI  +R+SLWYEHT  +  EE+F  PE+L+CV+R+RSI DE WKIYSGEEVADM+GVHL
Sbjct: 661 -DIHAYRMSLWYEHTAGRGEEELFEEPESLECVQRIRSIGDEMWKIYSGEEVADMEGVHL 720

Query: 723 VTYPVKVKQDGSIEDLEEN-GGHFPDTKCPIKGRRSMFLPPIFTT 762
           +TYPV VK+DG +ED  +  GGHFPDTK P+KG+RS  LPPIFTT
Sbjct: 721 LTYPVCVKEDGEVEDAVDGVGGHFPDTKTPVKGKRSKVLPPIFTT 764

BLAST of CmaCh01G000680 vs. NCBI nr
Match: gi|566160609|ref|XP_006385352.1| (Phospholipase D epsilon family protein [Populus trichocarpa])

HSP 1 Score: 1162.1 bits (3005), Expect = 0.0e+00
Identity = 525/760 (69.08%), Postives = 636/760 (83.68%), Query Frame = 1

Query: 3   VQGEHTFFHGTLEVTVFHATTYTPSSPLDCIFSGGKQSYVTIKINNIEVARTNHEYDRVW 62
           ++G+  F HGTLE T+F AT YTP  P +CIF  G  +YVTIKI N +VA+T+HE DRVW
Sbjct: 1   MEGKQKFLHGTLEATIFDATPYTPPFPFNCIFMNGSPTYVTIKIGNKKVAKTSHECDRVW 60

Query: 63  NQTFRVLCAHPLTSTITITMRTTRSVLGRFQIQAQQILKESSFINGFFPLLMENGKPSPE 122
           NQTF +LCAH L STITITM+T  S+LG+F IQA +I+ E+S INGFFPL+MENGKP+P+
Sbjct: 61  NQTFHILCAHSLDSTITITMKTKCSILGKFHIQAHKIVTEASLINGFFPLVMENGKPNPQ 120

Query: 123 LRLRFMLWFQPAVYELSWKKVLENGEYKGLRNVTFPLRSNCHVTLYQDAHHVSTFQPPFH 182
           L+LRFMLWF+PA  E +W K+L+NGE++GLRN TFP RSNCHVTLYQDAHH S+F+PPF 
Sbjct: 121 LKLRFMLWFKPAELETTWAKILDNGEFQGLRNATFPQRSNCHVTLYQDAHHCSSFKPPFD 180

Query: 183 GSSAPRRLWEDVYKAIDNAKHLVYIAGWSFNPKMVLVRDPQTDIPYALGVKLGELLKQKA 242
              +P +LWEDVYKA++ AKHL+YIAGWSFNPKMVLVRDP+TD+P+A GV LGELLK+K 
Sbjct: 181 LCGSPTKLWEDVYKAVEGAKHLIYIAGWSFNPKMVLVRDPETDMPHARGVMLGELLKRKG 240

Query: 243 EEGVAVRLMIWDDETSLPVIKNAGIMKTHDENARAYFSNSKVVCRLCPKLHPMSPPIFSH 302
           EEGVAVR+M+WDDETSLP IKN G+M  HDE+A AYF ++KV+C+LCP+LH   P +F+H
Sbjct: 241 EEGVAVRVMLWDDETSLPFIKNKGVMGVHDEDAFAYFKHTKVICKLCPRLHHKFPTLFAH 300

Query: 303 HQKTITVDAQTHINARNREIMSFIGGLDLCDGRYDTEQHSLFHTLNTESHCCDFYQTSIS 362
           HQKTITVDA+   +   REIMSF+GGLDLCDGRYDTE+HSLFHTLNTESHC DFYQT+I+
Sbjct: 301 HQKTITVDARARDSISEREIMSFVGGLDLCDGRYDTERHSLFHTLNTESHCFDFYQTNIA 360

Query: 363 GAKLQKGGPREPWHDVHARVTGEAAWDILTNFEQRWTKQSDASLLVPTSILLKLM-PPLE 422
           GA L+KGGPREPWHD HA + G+AA D+LTNFEQRW KQ D S+LVP S +  LM  P  
Sbjct: 361 GASLRKGGPREPWHDAHACIVGQAALDVLTNFEQRWNKQCDGSVLVPISSIPNLMHQPFP 420

Query: 423 SNTNPQKDWNVQVFRSIDHLSASQVFRNLTVERTIHEAYVEAIRRAERFIYIENQYFIGG 482
           S+ +  ++W VQVFRSIDH+SA  + RNL VER+IHEAYVEAIRRAERFIYIENQYFIGG
Sbjct: 421 SSVSNDRNWKVQVFRSIDHVSAIHLARNLRVERSIHEAYVEAIRRAERFIYIENQYFIGG 480

Query: 483 CHLWDKDQHCGCTNLIPIEIALKVANKIKAREKFAVYVVIPMWPEGPPESESVEDMLHWT 542
           C LWD+D+HCGCTNLIPIEIALKV NKI+A+E+FAVY+++PMWPEG P+SE V+D+LHWT
Sbjct: 481 CQLWDEDRHCGCTNLIPIEIALKVVNKIRAKERFAVYILMPMWPEGVPDSEPVQDILHWT 540

Query: 543 RQTMTMMYRLIGEAIQETGEKAHPRDYLNFFCLANREEERKWDFVPPHSPQHATEYWSAQ 602
           RQTM MMY+L+GEA+QE+GE  HPRDYLNFFCLANREEE K + VPP+SP  +T+YW+AQ
Sbjct: 541 RQTMAMMYKLVGEALQESGEPGHPRDYLNFFCLANREEENKGELVPPYSPHPSTQYWNAQ 600

Query: 603 HRRRFMVYVHSKVMIVDDLYLLIGSANVNQRSMDGERDTEIAIGCYQLENDGKELPNGRD 662
             RRFMVYVHSK+MIVDD Y+LIGSANVNQRSMDG RDTEIAIGCYQ +N G+   N RD
Sbjct: 601 KHRRFMVYVHSKLMIVDDAYMLIGSANVNQRSMDGRRDTEIAIGCYQPKN-GENTRNPRD 660

Query: 663 ISTFRLSLWYEHTQRFEEVFLNPENLQCVERVRSIADESWKIYSGEEVADMKGVHLVTYP 722
           I  +R+SLWYEHT   EE+FL PE+L C +R+R + ++ W +Y+GEEV DM+GVHLV YP
Sbjct: 661 ILAYRMSLWYEHTGLDEEIFLEPESLACAQRMRLVGEQMWNVYAGEEVVDMEGVHLVNYP 720

Query: 723 VKVKQDGSIEDLEENGGHFPDTKCPIKGRRSMFLPPIFTT 762
           ++V +DG++EDL + GG+FPDTK P+KGRRS  LPPIFTT
Sbjct: 721 LRVTKDGAVEDLVDGGGNFPDTKSPVKGRRSNMLPPIFTT 759

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PLDA4_ARATH2.2e-28359.82Phospholipase D alpha 4 OS=Arabidopsis thaliana GN=PLDALPHA4 PE=2 SV=1[more]
PLDA1_MAIZE3.3e-17841.11Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 SV=1[more]
PLDA1_ORYSJ3.7e-17741.18Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica GN=PLD1 PE=1 SV=2[more]
PLDA1_TOBAC9.3e-17340.35Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PLD1 PE=2 SV=2[more]
PLDA1_CYNCA2.1e-17240.35Phospholipase D alpha 1 OS=Cynara cardunculus GN=PLD1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0LGB5_CUCSA0.0e+0087.78Phospholipase D OS=Cucumis sativus GN=Csa_3G848850 PE=3 SV=1[more]
B9MWR1_POPTR0.0e+0069.08Phospholipase D OS=Populus trichocarpa GN=POPTR_0003s02990g PE=3 SV=1[more]
B9SN47_RICCO0.0e+0069.09Phospholipase D OS=Ricinus communis GN=RCOM_0312720 PE=3 SV=1[more]
M5XB63_PRUPE0.0e+0070.57Phospholipase D OS=Prunus persica GN=PRUPE_ppa022510mg PE=3 SV=1[more]
A0A061GBY3_THECC0.0e+0069.28Phospholipase D OS=Theobroma cacao GN=TCM_029016 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G55180.11.3e-28459.82 phospholipase D alpha 4[more]
AT5G25370.13.4e-17341.40 phospholipase D alpha 3[more]
AT3G15730.15.8e-17341.95 phospholipase D alpha 1[more]
AT1G52570.19.3e-17141.82 phospholipase D alpha 2[more]
AT2G42010.14.5e-14940.96 phospholipase D beta 1[more]
Match NameE-valueIdentityDescription
gi|449458181|ref|XP_004146826.1|0.0e+0089.62PREDICTED: phospholipase D epsilon [Cucumis sativus][more]
gi|659093575|ref|XP_008447602.1|0.0e+0089.49PREDICTED: phospholipase D epsilon [Cucumis melo][more]
gi|700204694|gb|KGN59827.1|0.0e+0087.78hypothetical protein Csa_3G848850 [Cucumis sativus][more]
gi|1009112032|ref|XP_015866270.1|0.0e+0071.63PREDICTED: phospholipase D alpha 4 [Ziziphus jujuba][more]
gi|566160609|ref|XP_006385352.1|0.0e+0069.08Phospholipase D epsilon family protein [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000008C2_dom
IPR001736PLipase_D/transphosphatidylase
IPR011402PLipase_D_pln
IPR015679PLipase_D_fam
IPR024632PLipase_D_C
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO:0003824catalytic activity
GO:0004630phospholipase D activity
GO:0005509calcium ion binding
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: Biological Process
TermDefinition
GO:0046470phosphatidylcholine metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051301 cell division
biological_process GO:0045848 positive regulation of nitrogen utilization
biological_process GO:0048364 root development
biological_process GO:0016049 cell growth
biological_process GO:0009791 post-embryonic development
biological_process GO:0006970 response to osmotic stress
biological_process GO:0009395 phospholipid catabolic process
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0051365 cellular response to potassium ion starvation
biological_process GO:0016036 cellular response to phosphate starvation
biological_process GO:0006995 cellular response to nitrogen starvation
biological_process GO:0016042 lipid catabolic process
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0005515 protein binding
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh01G000680.1CmaCh01G000680.1mRNA


Analysis Name: InterPro Annotations of Cucurbita maxima
Date Performed: 2017-05-20
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainGENE3DG3DSA:2.60.40.150coord: 11..127
score: 1.
IPR000008C2 domainPFAMPF00168C2coord: 12..112
score: 5.
IPR000008C2 domainunknownSSF49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 9..129
score: 1.1
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 300..335
score: 1.0E-5coord: 609..633
score: 7.
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 606..633
score: 1.0E-5coord: 300..335
score:
IPR001736Phospholipase D/TransphosphatidylasePROFILEPS50035PLDcoord: 606..633
score: 14
IPR011402Phospholipase D, plantPIRPIRSF036470PLD_plantcoord: 2..761
score: 1.6E
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 191..761
score: 0.0coord: 1..174
score:
IPR024632Phospholipase D, C-terminalPFAMPF12357PLD_Ccoord: 678..750
score: 4.0
NoneNo IPR availableGENE3DG3DSA:3.30.870.10coord: 456..644
score: 1.2E-16coord: 157..340
score: 3.4E-22coord: 373..409
score: 3.4
NoneNo IPR availablePANTHERPTHR18896:SF79PHOSPHOLIPASE D EPSILONcoord: 1..174
score: 0.0coord: 191..761
score:
NoneNo IPR availableunknownSSF56024Phospholipase D/nucleasecoord: 602..688
score: 8.05E-28coord: 425..556
score: 8.05E-28coord: 372..409
score: 2.43E-30coord: 149..342
score: 2.43

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
CmaCh01G000680Cucurbita pepo (Zucchini)cmacpeB475
CmaCh01G000680Cucurbita pepo (Zucchini)cmacpeB466
CmaCh01G000680Cucurbita pepo (Zucchini)cmacpeB493
CmaCh01G000680Cucurbita pepo (Zucchini)cmacpeB496
CmaCh01G000680Bottle gourd (USVL1VR-Ls)cmalsiB402
CmaCh01G000680Bottle gourd (USVL1VR-Ls)cmalsiB412
CmaCh01G000680Bottle gourd (USVL1VR-Ls)cmalsiB444
CmaCh01G000680Cucumber (Gy14) v2cgybcmaB112
CmaCh01G000680Cucumber (Gy14) v2cgybcmaB202
CmaCh01G000680Cucumber (Gy14) v2cgybcmaB930
CmaCh01G000680Melon (DHL92) v3.6.1cmamedB457
CmaCh01G000680Melon (DHL92) v3.6.1cmamedB467
CmaCh01G000680Melon (DHL92) v3.6.1cmamedB495
CmaCh01G000680Silver-seed gourdcarcmaB0124
CmaCh01G000680Silver-seed gourdcarcmaB0157
CmaCh01G000680Silver-seed gourdcarcmaB1420
CmaCh01G000680Cucumber (Chinese Long) v3cmacucB0520
CmaCh01G000680Cucumber (Chinese Long) v3cmacucB0525
CmaCh01G000680Cucumber (Chinese Long) v3cmacucB0587
CmaCh01G000680Watermelon (97103) v2cmawmbB452
CmaCh01G000680Watermelon (97103) v2cmawmbB464
CmaCh01G000680Watermelon (97103) v2cmawmbB517
CmaCh01G000680Wax gourdcmawgoB0607
CmaCh01G000680Wax gourdcmawgoB0630
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB107
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB144
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB254
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB360
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB430
CmaCh01G000680Cucurbita maxima (Rimu)cmacmaB441
CmaCh01G000680Cucumber (Gy14) v1cgycmaB0985
CmaCh01G000680Cucurbita moschata (Rifu)cmacmoB448
CmaCh01G000680Cucurbita moschata (Rifu)cmacmoB450
CmaCh01G000680Cucurbita moschata (Rifu)cmacmoB461
CmaCh01G000680Cucurbita moschata (Rifu)cmacmoB466
CmaCh01G000680Cucurbita moschata (Rifu)cmacmoB491
CmaCh01G000680Wild cucumber (PI 183967)cmacpiB437
CmaCh01G000680Wild cucumber (PI 183967)cmacpiB494
CmaCh01G000680Cucumber (Chinese Long) v2cmacuB434
CmaCh01G000680Cucumber (Chinese Long) v2cmacuB490
CmaCh01G000680Melon (DHL92) v3.5.1cmameB397
CmaCh01G000680Melon (DHL92) v3.5.1cmameB407
CmaCh01G000680Watermelon (Charleston Gray)cmawcgB383
CmaCh01G000680Watermelon (Charleston Gray)cmawcgB402
CmaCh01G000680Watermelon (97103) v1cmawmB413
CmaCh01G000680Watermelon (97103) v1cmawmB427