Cla97C05G093370 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C05G093370
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	3-ketodihydrosphingosine reductase
Location	Cla97Chr05 : 12363190 .. 12365300 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGATTTCAATTATGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGCTCCGTTAAGATTCCTCTAAAGAATCGCCATGTCTTCATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCATTGGAGGGGGCGCGAGTCTCCATCCTTGCGCGTTCTTTCAAGAAGCTCGAGGAAGCCAAAGACGCAATCCGACTATCCACTGGCATTGATGTGGCGATCCACGCTGCCGATGTTCGAGATTACGATGCCATTTCAAAGGCTGTAGATGAAGCAGGCCCAATTGATGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCATCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTAATTGATATAAAGGATGGCGAAATGACCTTGGTTAACAAAAAGTACTTTTAATGGTTTTATGAAGTGTTCATGTTCGAAACTTCTTCAATTATTAGGGTCAATTCAATGGCATTAATAACATGCAAACACTCGCAAGTTGTAAGTTAAGTGGAATGGTAAATCTTTTACTCCTGAAGATCTTCTTGTAAATTCGTGTAGGTGGGTATTTATGGTTATACAGCCTATTCCGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTTCCTCCGGACACTGACACTCCTGGATTTGCAGAAGGTATGTGTAAAGCAAAATCTGGTTTACCTTATGGAATTGTTATGCTAGTTCTTATTAGGGTTTCAATATTTATCCGATGGGTTAGAGAATTCTTAGGTTAGTTTTCATTTCATTGGTTTGTGTCAAGAAATGATAGGAAATGTCTTGGAAGGATAAGCTAACTAGACTATCACCTGGCCAGATTTTATTTGAGTTAGTAACCTTCTTTATTAGATATCTCAGGTAACAAAAATTCTTAAATTTATACGATAGAATCTTTTATTAGCTTGTTTCTTCAATTATCAGTTGCTTGTCGTTAATTGCAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAATTTTGAGGGAACATTATTGTCGATAGCAACTGCTGGCTTGTCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCACAAAGAAATAGTAAGTCTTCATTTAAATGATGAAACCATCACACCTTGTTCATTCCATTACTAATATATGTGCTAGACGCCTCGTTGTGTGGTTGACTATTAAAAATGACATTTTTATCAGGCAGCAATGAGACTTGGTTAAAATAGTCAAACTTTCACACATTTCAAAAGCTAATTAGATTGCTTTTTTTTTTTTTTTTTTTTTTAGTTACATAACTATGCAATCTCTGGTTCTTGGTCTCAATTTTGCTTTTAGATTCTTTCTTTTCTAGTTTCTAACATCTGATGATGTGAATGATTCTGTCTTCCTTTCGAGCTTTTGCTTCATTGTGAATGTCATATTAGATCCGTATTCTAATTATCATATCTTTAACATATATTCTCATACTGTTGATGTCTGTTACATCTTGTCTTAAGCTGATCCATGCTGTGAGTTGACCAGTTTCCTACCTAAGTAACAGAAAATCTACATTTAACCTAATAAATTTAATTATGTTATTGTGAGGTCAAATATCTGGATAAGCCTGGAACTTTTATCAAAGTTCATTTCTTTGTTTGTTCCTTTCTCTCTAAATGTAACTTTAAGCCATTTGATGAGCATGTGTTGGTAAACTTATCTATATCCTTTGGCCAGGAACGTAG

mRNA sequence

ATGGCGGATTTCAATTATGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGCTCCGTTAAGATTCCTCTAAAGAATCGCCATGTCTTCATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCATTGGAGGGGGCGCGAGTCTCCATCCTTGCGCGTTCTTTCAAGAAGCTCGAGGAAGCCAAAGACGCAATCCGACTATCCACTGGCATTGATGTGGCGATCCACGCTGCCGATGTTCGAGATTACGATGCCATTTCAAAGGCTGTAGATGAAGCAGGCCCAATTGATGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCATCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTGGGTATTTATGGTTATACAGCCTATTCCGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTTCCTCCGGACACTGACACTCCTGGATTTGCAGAAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAATTTTGAGGGAACATTATTGTCGATAGCAACTGCTGGCTTGTCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCACAAAGAAATAGAACGTAG

Coding sequence (CDS)

ATGGCGGATTTCAATTATGCCTTCATCTCTCTCCTTCTTCTTCTTCCTTTCGCACTCCTCGTTTTCCTATACCTAATTGTGCGTCCACGCTCCGTTAAGATTCCTCTAAAGAATCGCCATGTCTTCATTACCGGCGGATCGAGCGGAATCGGCCTCGCTTTGGCCCACCAGGCCGCATTGGAGGGGGCGCGAGTCTCCATCCTTGCGCGTTCTTTCAAGAAGCTCGAGGAAGCCAAAGACGCAATCCGACTATCCACTGGCATTGATGTGGCGATCCACGCTGCCGATGTTCGAGATTACGATGCCATTTCAAAGGCTGTAGATGAAGCAGGCCCAATTGATGTTCTTATTGTGAATCAAGGCGTGTTCGTACCTCAAGAACTGGCGGAGCAGGAATTGGATGAGGTGAAATTCATGTTAGATGTGAATTTATTGGGCACATTTAACATGATTAAGGCTGCCCTACCTGCGATGAAGAATAGAGTGGACCATCGACCCGCATCGATCGCTCTCATGTCTTCTCAGGCCGGTCAGGTGGGTATTTATGGTTATACAGCCTATTCCGCTAGTAAGTTTGGGATTCGAGGTTTGGCAGAAGCCTTGCAACAGGAGGTCATTGGAGATGACATACATATTTCTCTTATATTTCCTCCGGACACTGACACTCCTGGATTTGCAGAAGAGCAAAAGAAAAGGCCTGAATTGACCACTATCATTGCTGCTGGAGGAGGCAGTATGAAACCCGAGTTAGTTGCCAAAAAAGCTTTTAATGGCATTAAATCTGGTAGTTTTATAATTCCCTGCAATTTTGAGGGAACATTATTGTCGATAGCAACTGCTGGCTTGTCTCCTCAGAGATCATTCTTAATGGCATTCCTTGAAGTTGTTGGTGCTGGAGTAGTAAGGATTGTGGCCTTGTGTTTTCAATGGGTTTGGTATGGAAGTATTGAAAAATGGCATGCACAAAGAAATAGAACGTAG

Protein sequence

MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTTIIAAGGGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQRNRT

Homology

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BLAST of Cla97C05G093370 vs. NCBI nr
Match: XP_004134147.1 (PREDICTED: 3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 hypothetical protein Csa_3G149900 [Cucumis sativus])

HSP 1 Score: 579.7 bits (1493), Expect = 6.2e-162
Identity = 308/326 (94.48%), Postives = 317/326 (97.24%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MAD N AFISL+LLLPFALL+FLYLIVRPR V+I LK+RHVFITGGSSGIGLALAHQAA 
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS KKLEEAKDAIRLSTGIDVA++AADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVD RPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXX 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTXXXX
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTXXXX 240

Query: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGV 300
           XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSF MAF+EVVGAGV
Sbjct: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 VRIVALCFQWVWYGSIEKWHAQRNRT 327
           +RIVALCFQWVWYGSIEKWHAQRNRT
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326

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BLAST of Cla97C05G093370 vs. NCBI nr
Match: XP_023539235.1 (3-dehydrosphinganine reductase TSC10A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 554.3 bits (1427), Expect = 2.8e-154
Identity = 284/326 (87.12%), Postives = 301/326 (92.33%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MADFNYAFISL+LLLP ALL+FLYLIVRPRSV IPLKNRHVFITGGSSGIGLALAHQAA 
Sbjct: 1   MADFNYAFISLVLLLPLALLIFLYLIVRPRSVNIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS KKLEEAKDAIRLSTGIDVA+ AADVRDYDAISKAVDEAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVFAADVRDYDAISKAVDEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVG 180
           GVFVPQELAEQ++DEVKFMLDVNLLG F+MIKAALP MKNRVD RPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQDMDEVKFMLDVNLLGAFHMIKAALPTMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXX 240
           +YGYTAYSASKFG+RGLAEALQQEVIGD+IHISLIFPPDTDTPG  EE+K+RPELT    
Sbjct: 181 VYGYTAYSASKFGLRGLAEALQQEVIGDNIHISLIFPPDTDTPGLVEEEKRRPELTNIIA 240

Query: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGV 300
              G MK ELVAKKA NGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFLMAFLEVV AG+
Sbjct: 241 AGGGRMKAELVAKKALNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFLMAFLEVVTAGI 300

Query: 301 VRIVALCFQWVWYGSIEKWHAQRNRT 327
           +RIV LCFQWVWY SI+KWHAQ+ RT
Sbjct: 301 IRIVGLCFQWVWYDSIQKWHAQKKRT 326

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BLAST of Cla97C05G093370 vs. NCBI nr
Match: XP_022973673.1 (3-dehydrosphinganine reductase TSC10A [Cucurbita maxima])

HSP 1 Score: 553.9 bits (1426), Expect = 3.6e-154
Identity = 283/326 (86.81%), Postives = 301/326 (92.33%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MADFNYAFISL+LLLP ALL+FLYLIVRPRSV IPLKNRHVFITGGSSGIGLALAHQAA 
Sbjct: 1   MADFNYAFISLVLLLPLALLIFLYLIVRPRSVNIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS KKLEEAKDA+RLSTGIDVA+ AADVRDYDAISKAVDEAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAVRLSTGIDVAVFAADVRDYDAISKAVDEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVG 180
           GVFVPQELAEQ++DEVKFMLDVNLLG F+MIKAALP MKNRVD RPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQDMDEVKFMLDVNLLGAFHMIKAALPTMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXX 240
           +YGYTAYSASKFG+RGLAEALQQEVIGD+IHISLIFPPDTDTPG  EE+K+RPELT    
Sbjct: 181 VYGYTAYSASKFGLRGLAEALQQEVIGDNIHISLIFPPDTDTPGLVEEEKRRPELTNIIA 240

Query: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGV 300
              G MK ELVAKKA NGIKSGSFIIPCNFEG LLS+ATAGLSPQRSFLMAFLEVV AG+
Sbjct: 241 AGGGRMKAELVAKKALNGIKSGSFIIPCNFEGALLSVATAGLSPQRSFLMAFLEVVTAGI 300

Query: 301 VRIVALCFQWVWYGSIEKWHAQRNRT 327
           +RIV LCFQWVWY SI+KWHAQ+ RT
Sbjct: 301 IRIVGLCFQWVWYDSIQKWHAQKKRT 326

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BLAST of Cla97C05G093370 vs. NCBI nr
Match: XP_022138240.1 (3-dehydrosphinganine reductase TSC10A-like [Momordica charantia])

HSP 1 Score: 546.2 bits (1406), Expect = 7.5e-152
Identity = 284/327 (86.85%), Postives = 300/327 (91.74%), Query Frame = 0


Query: 1   MADFNYAFISL-LLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAA 60
           MA+FNYAFISL LLLLPFALLVFLY IVRPR VKIPLKNRHVFITGGSSGIGLALAHQAA
Sbjct: 1   MAEFNYAFISLVLLLLPFALLVFLYFIVRPRPVKIPLKNRHVFITGGSSGIGLALAHQAA 60

Query: 61  LEGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVN 120
            EGARVSILARS  KLE+AKDAIRLSTGIDVA++AADVRDYDAIS+A DEAGPIDVLIVN
Sbjct: 61  SEGARVSILARSLNKLEDAKDAIRLSTGIDVAVYAADVRDYDAISRAADEAGPIDVLIVN 120

Query: 121 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQV 180
           QGVFVPQELAEQ +DEVKFMLDVNL+GTFNMIKAALPAMKNRVD RPASIALMSSQAGQV
Sbjct: 121 QGVFVPQELAEQGMDEVKFMLDVNLVGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXX 240
           GIYGYTAYSASKFG+RGLAEALQQEVIGDDIHIS+IFPPDTDTPG AEE K+RPELT   
Sbjct: 181 GIYGYTAYSASKFGLRGLAEALQQEVIGDDIHISVIFPPDTDTPGLAEEHKRRPELTSII 240

Query: 241 XXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAG 300
               GSMK E VAKK  NGIKSGSFI+PCNFEG LLSI+TAGLSPQ SF MAFLEVV AG
Sbjct: 241 AAGGGSMKAEAVAKKTLNGIKSGSFIVPCNFEGALLSISTAGLSPQNSFWMAFLEVVFAG 300

Query: 301 VVRIVALCFQWVWYGSIEKWHAQRNRT 327
           ++RIVALCFQWVWYGSIEKW+AQ+ RT
Sbjct: 301 IIRIVALCFQWVWYGSIEKWNAQKKRT 327

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BLAST of Cla97C05G093370 vs. NCBI nr
Match: XP_022933748.1 (3-dehydrosphinganine reductase TSC10A [Cucurbita moschata] >XP_022933757.1 3-dehydrosphinganine reductase TSC10A [Cucurbita moschata] >XP_022933763.1 3-dehydrosphinganine reductase TSC10A [Cucurbita moschata])

HSP 1 Score: 545.8 bits (1405), Expect = 9.9e-152
Identity = 281/325 (86.46%), Postives = 298/325 (91.69%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MADFNYAFISL+LLLP ALL+FLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAA 
Sbjct: 1   MADFNYAFISLVLLLPLALLIFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAS 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS KKLEEAKDAI LSTGIDVA+ AADVRDYDAISKAVDEAGPIDVL+VNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIGLSTGIDVAVFAADVRDYDAISKAVDEAGPIDVLVVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVG 180
           GVFVPQELAEQ++DEVKFMLDVNLLG F+MIKAALP MK RVD RPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQDMDEVKFMLDVNLLGAFHMIKAALPTMKTRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXX 240
           +YGYTAYSASKFG+RGLAEALQQEVIGD+IHISLIFPPDTDTPG  EE+K+RPELT    
Sbjct: 181 VYGYTAYSASKFGLRGLAEALQQEVIGDNIHISLIFPPDTDTPGLVEEEKRRPELTNIIA 240

Query: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGV 300
              G MK ELVAKKA NGIKSGSFIIPCNFEG LLS+ATAGLSPQRS LMAFLEVV AG+
Sbjct: 241 AGGGRMKAELVAKKALNGIKSGSFIIPCNFEGALLSVATAGLSPQRSCLMAFLEVVTAGI 300

Query: 301 VRIVALCFQWVWYGSIEKWHAQRNR 326
           +RIV LCFQWVWY SI+KWHAQ+ R
Sbjct: 301 IRIVGLCFQWVWYDSIQKWHAQKKR 325

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BLAST of Cla97C05G093370 vs. TrEMBL
Match: tr|A0A0A0L506|A0A0A0L506_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 4.1e-162
Identity = 308/326 (94.48%), Postives = 317/326 (97.24%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MAD N AFISL+LLLPFALL+FLYLIVRPR V+I LK+RHVFITGGSSGIGLALAHQAA 
Sbjct: 1   MADLNLAFISLVLLLPFALLLFLYLIVRPRPVRISLKSRHVFITGGSSGIGLALAHQAAT 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS KKLEEAKDAIRLSTGIDVA++AADVRDYDAI KAVDEAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAADVRDYDAILKAVDEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVG 180
           GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVD RPASIALMSSQAGQVG
Sbjct: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDRRPASIALMSSQAGQVG 180

Query: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXX 240
           IYGYTAYSASKFGIRGLAEALQQEVIGDDIH+SLIFPPDTDTPGFAEEQKKRPELTXXXX
Sbjct: 181 IYGYTAYSASKFGIRGLAEALQQEVIGDDIHVSLIFPPDTDTPGFAEEQKKRPELTXXXX 240

Query: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGV 300
           XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSF MAF+EVVGAGV
Sbjct: 241 XXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFFMAFIEVVGAGV 300

Query: 301 VRIVALCFQWVWYGSIEKWHAQRNRT 327
           +RIVALCFQWVWYGSIEKWHAQRNRT
Sbjct: 301 IRIVALCFQWVWYGSIEKWHAQRNRT 326

[Close]

BLAST of Cla97C05G093370 vs. TrEMBL
Match: tr|A0A1S4DT08|A0A1S4DT08_CUCME (3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 3.1e-146
Identity = 277/287 (96.52%), Postives = 282/287 (98.26%), Query Frame = 0


Query: 37  KNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAAD 96
           K+RHVFITGGSSGIGLALAHQAA EGARVSILARS KKLEEAKDAIRLSTGIDVA++AAD
Sbjct: 37  KSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAAD 96

Query: 97  VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP 156
           VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP
Sbjct: 97  VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP 156

Query: 157 AMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF 216
           AMKNRVD RPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF
Sbjct: 157 AMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF 216

Query: 217 PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLS 276
           PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS
Sbjct: 217 PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLS 276

Query: 277 IATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQR 324
           +ATAGLSPQRSF MAFLEVVGAGV+RIVALCFQWVWYGSIEKWHAQR
Sbjct: 277 VATAGLSPQRSFFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQR 323

[Close]

BLAST of Cla97C05G093370 vs. TrEMBL
Match: tr|A0A1S3AX60|A0A1S3AX60_CUCME (3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483732 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 3.1e-146
Identity = 277/287 (96.52%), Postives = 282/287 (98.26%), Query Frame = 0


Query: 37  KNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAAD 96
           K+RHVFITGGSSGIGLALAHQAA EGARVSILARS KKLEEAKDAIRLSTGIDVA++AAD
Sbjct: 37  KSRHVFITGGSSGIGLALAHQAATEGARVSILARSLKKLEEAKDAIRLSTGIDVAVYAAD 96

Query: 97  VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP 156
           VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP
Sbjct: 97  VRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALP 156

Query: 157 AMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF 216
           AMKNRVD RPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF
Sbjct: 157 AMKNRVDRRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIF 216

Query: 217 PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLS 276
           PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEG LLS
Sbjct: 217 PPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGALLS 276

Query: 277 IATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQR 324
           +ATAGLSPQRSF MAFLEVVGAGV+RIVALCFQWVWYGSIEKWHAQR
Sbjct: 277 VATAGLSPQRSFFMAFLEVVGAGVIRIVALCFQWVWYGSIEKWHAQR 323

[Close]

BLAST of Cla97C05G093370 vs. TrEMBL
Match: tr|A0A2I4G9E7|A0A2I4G9E7_9ROSI (3-dehydrosphinganine reductase TSC10A-like isoform X1 OS=Juglans regia OX=51240 GN=LOC109005878 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 8.9e-133
Identity = 250/325 (76.92%), Postives = 280/325 (86.15%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MAD N A+++LLLLLP ++L FLYLIVRPR V+IP+KNRHVFITGGSSGIGLALA QAA 
Sbjct: 1   MADPNLAYLALLLLLPLSILFFLYLIVRPRPVRIPIKNRHVFITGGSSGIGLALAQQAAS 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS  KLE+AK AIRL+TG+DVA+ AADVRDYDA++KAV+EAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLDKLEDAKQAIRLATGVDVAVFAADVRDYDAVTKAVEEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMK-NRVDHRPASIALMSSQAGQV 180
           GVFVPQEL  Q LDEVKFM+DVNL+G FNMIKAALPAMK NR D  PASIALMSSQAGQV
Sbjct: 121 GVFVPQELERQGLDEVKFMVDVNLIGAFNMIKAALPAMKLNRSDRGPASIALMSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXX 240
           GIYGYTAYSASKFG+RGLAEALQQEVI DDIH+SLIFPPDTDTPG  EE K+RP+LT   
Sbjct: 181 GIYGYTAYSASKFGLRGLAEALQQEVIADDIHVSLIFPPDTDTPGLVEENKRRPQLTAII 240

Query: 241 XXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAG 300
               G+MK + VA KA +GIKS SFI+PCNF+G LLSIATAGLSPQRSFLMAF+EVV  G
Sbjct: 241 AASSGAMKADEVAMKALSGIKSSSFIVPCNFDGLLLSIATAGLSPQRSFLMAFVEVVATG 300

Query: 301 VVRIVALCFQWVWYGSIEKWHAQRN 325
           ++R VALC+QW W  SIEKW  + N
Sbjct: 301 IIRFVALCYQWNWCRSIEKWTLKNN 325

[Close]

BLAST of Cla97C05G093370 vs. TrEMBL
Match: tr|A0A2I4G9D0|A0A2I4G9D0_9ROSI (3-dehydrosphinganine reductase TSC10A-like isoform X2 OS=Juglans regia OX=51240 GN=LOC109005878 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 8.9e-133
Identity = 252/327 (77.06%), Postives = 282/327 (86.24%), Query Frame = 0


Query: 1   MADFNYAFISLLLLLPFALLVFLYLIVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAAL 60
           MAD N A+++LLLLLP ++L FLYLIVRPR V+IP+KNRHVFITGGSSGIGLALA QAA 
Sbjct: 1   MADPNLAYLALLLLLPLSILFFLYLIVRPRPVRIPIKNRHVFITGGSSGIGLALAQQAAS 60

Query: 61  EGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQ 120
           EGARVSILARS  KLE+AK AIRL+TG+DVA+ AADVRDYDA++KAV+EAGPIDVLIVNQ
Sbjct: 61  EGARVSILARSLDKLEDAKQAIRLATGVDVAVFAADVRDYDAVTKAVEEAGPIDVLIVNQ 120

Query: 121 GVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMK-NRVDHRPASIALMSSQAGQV 180
           GVFVPQEL  Q LDEVKFM+DVNL+G FNMIKAALPAMK NR D  PASIALMSSQAGQV
Sbjct: 121 GVFVPQELERQGLDEVKFMVDVNLIGAFNMIKAALPAMKLNRSDRGPASIALMSSQAGQV 180

Query: 181 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXX 240
           GIYGYTAYSASKFG+RGLAEALQQEVI DDIH+SLIFPPDTDTPG  EE K+RP+LT   
Sbjct: 181 GIYGYTAYSASKFGLRGLAEALQQEVIADDIHVSLIFPPDTDTPGLVEENKRRPQLTAII 240

Query: 241 XXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAG 300
               G+MK + VA KA +GIKS SFI+PCNF+G LLSIATAGLSPQRSFLMAF+EVV  G
Sbjct: 241 AASSGAMKADEVAMKALSGIKSSSFIVPCNFDGLLLSIATAGLSPQRSFLMAFVEVVATG 300

Query: 301 VVRIVALCFQWVWYGSIEKWHAQRNRT 327
           ++R VALC+QW W  SIEKW   +NRT
Sbjct: 301 IIRFVALCYQWNWCRSIEKW-TLKNRT 326

[Close]

BLAST of Cla97C05G093370 vs. Swiss-Prot
Match: sp|Q0WRJ2|TC10A_ARATH (3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 9.5e-98
Identity = 181/299 (60.54%), Postives = 224/299 (74.92%), Query Frame = 0


Query: 27  VRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLST 86
           VRPR +KIP+K+RH FITGGSSGIGLALAH+AA EGARVSILARS  KLEEAK +I+L+T
Sbjct: 28  VRPRPIKIPIKSRHAFITGGSSGIGLALAHRAASEGARVSILARSGSKLEEAKKSIQLAT 87

Query: 87  GIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLG 146
           G++VA  +ADVRDYDA+SKA+DE+GPIDVLIVNQGVF  +EL +   ++VKF +DVNL+G
Sbjct: 88  GVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFTAKELVKHSPEDVKFTIDVNLVG 147

Query: 147 TFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVI 206
           +FN+IKAALPAM                                 FG++GLA+ALQQEVI
Sbjct: 148 SFNVIKAALPAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGLQGLAQALQQEVI 207

Query: 207 GDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFII 266
            DDIH++LIFPPDT+TPGF EEQK RPE+T       GSM+ E VAKKA +GIK+G+F +
Sbjct: 208 SDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAIIAASSGSMETEEVAKKAMDGIKAGNFTV 267

Query: 267 PCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQRNR 326
            CNFEG LLS+AT G+SPQRSF +AFLEV+ AG +R++AL FQW WY +IEKW   + +
Sbjct: 268 SCNFEGFLLSLATTGMSPQRSFWLAFLEVITAGPIRLIALFFQWDWYKAIEKWSKTKTK 326

[Close]

BLAST of Cla97C05G093370 vs. Swiss-Prot
Match: sp|F4JZN6|TC10B_ARATH (3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 4.7e-97
Identity = 185/300 (61.67%), Postives = 227/300 (75.67%), Query Frame = 0


Query: 26  IVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLS 85
           IVRPRSV IP+K RHVFITGGSSGIGLALAH+A  EGA+VSILARS +KL EAK +I+L+
Sbjct: 25  IVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVSEGAKVSILARSTEKLAEAKRSIQLA 84

Query: 86  TGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLL 145
           TG++VA  +ADVRDYDA+SKA+DE+GPIDVLIVNQGVF+ +EL +Q  +EVKFM+DVNL 
Sbjct: 85  TGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFIGKELEKQSPEEVKFMIDVNLT 144

Query: 146 GTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEV 205
           G+FN+IKAALPAMK R    PASI                     KFG++GLA+ALQQEV
Sbjct: 145 GSFNVIKAALPAMKAREGRGPASIXXXXXXXXXXXXXXXXXXXXXKFGLQGLAQALQQEV 204

Query: 206 IGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFI 265
           I D IH++L+FPPDTDTPGF +E KKRPELT       GSMK   VAK  F+GIK+G F 
Sbjct: 205 ISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIAASSGSMKTNEVAKICFDGIKAGKFT 264

Query: 266 IPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQRNR 325
           + C+F G LLSIA+ G+SPQ SF +A  EV+  G++R+ +L FQW WY +IEKW +QRN+
Sbjct: 265 VTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGLIRLASLVFQWQWYKTIEKW-SQRNK 323

[Close]

BLAST of Cla97C05G093370 vs. Swiss-Prot
Match: sp|Q06136|KDSR_HUMAN (3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 3.9e-43
Identity = 114/327 (34.86%), Postives = 185/327 (56.57%), Query Frame = 0


Query: 9   ISLLLLLPFALLVFLYL-IVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSI 68
           ++   L+ F LL+++   ++ P+ + +P    HV +TGGSSGIG  +A +   +GA +++
Sbjct: 4   LAAAFLVAFVLLLYMVSPLISPKPLALP--GAHVVVTGGSSGIGKCIAIECYKQGAFITL 63

Query: 69  LARSFKKLEEAKDAIRLSTGID---VAIHAADV-RDYDAISKAVDEA----GPIDVLIVN 128
           +AR+  KL +AK  I + +  D   V   + DV +DY+ +   + +A    GP+D+L+  
Sbjct: 64  VARNEDKLLQAKKEIEMHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNC 123

Query: 129 QGVFVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQV 188
            G+ V  +  + E+   + ++ +N LG+    +A +  MK R   R   I  +SSQAGQ+
Sbjct: 124 AGMAVSGKFEDLEVSTFERLMSINYLGSVYPSRAVITTMKER---RVGRIVFVSSQAGQL 183

Query: 189 GIYGYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXX 248
           G++G+TAYSASKF IRGLAEALQ EV   +++I++ +PPDTDTPGFAEE + +P  T   
Sbjct: 184 GLFGFTAYSASKFAIRGLAEALQMEVKPYNVYITVAYPPDTDTPGFAEENRTKPLETRLI 243

Query: 249 XXXXGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAG 308
                  KPE VAK+       G+F      +G +LS  T G++P  S      +VV  G
Sbjct: 244 SETTSVCKPEQVAKQIVKDAIQGNFNSSLGSDGYMLSALTCGMAPVTSITEGLQQVVTMG 303

Query: 309 VVRIVALCFQWVWYGSIEKWHAQRNRT 327
           + R +AL +   +   + +   QR ++
Sbjct: 304 LFRTIALFYLGSFDSIVRRCMMQREKS 325

[Close]

BLAST of Cla97C05G093370 vs. Swiss-Prot
Match: sp|Q6GV12|KDSR_MOUSE (3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.7e-41
Identity = 110/304 (36.18%), Postives = 173/304 (56.91%), Query Frame = 0


Query: 14  LLPFALLVFLYL-IVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSF 73
           L+ F LL+++   ++ P+ + +P    HV +TGGSSGIG  +A +   +GA ++++AR+ 
Sbjct: 9   LVAFVLLLYMVSPLISPKPLALP--GAHVVVTGGSSGIGKCIAIECYKQGAFITLVARNE 68

Query: 74  KKLEEAKDAIRLSTGID---VAIHAADV-RDYDAISKAVDEA----GPIDVLIVNQGVFV 133
            KL +AK  I   +  D   V   + DV +DY+ +   + +A    GP+D+L+   G  +
Sbjct: 69  DKLLQAKKDIEKHSINDKQVVLCISVDVSQDYNQVENVIKQAQEKLGPVDMLVNCAGTSM 128

Query: 134 PQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGY 193
             +  E E+   + ++ +N LG+    +A +  MK R   R   I  +SSQAGQ+G++G+
Sbjct: 129 SGKFEELEVSSFEKLMSINYLGSVYPSRAVITTMKER---RVGRIVFVSSQAGQLGLFGF 188

Query: 194 TAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXXXG 253
           TAYS+SKF IRGLAEALQ EV   ++++++ +PPDTDTPG AEE K +P  T        
Sbjct: 189 TAYSSSKFAIRGLAEALQMEVKPYNVYVTVAYPPDTDTPGLAEENKTKPLETRLISETTA 248

Query: 254 SMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIV 309
             KPE VAK+       G+F      +G +LS  T G++P  S      +VV  G+ R +
Sbjct: 249 ICKPEQVAKQIVKDAIQGNFNSSIGSDGYMLSSLTCGMAPVTSITEGLQQVVTMGLFRTI 307

[Close]

BLAST of Cla97C05G093370 vs. Swiss-Prot
Match: sp|Q2KIJ5|KDSR_BOVIN (3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.8e-41
Identity = 106/306 (34.64%), Postives = 177/306 (57.84%), Query Frame = 0


Query: 14  LLPFALLVFLYL-IVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSF 73
           L+ F LL+++   ++ P+ + +P    HV +TGGSSGIG  +A +   +GA ++++AR+ 
Sbjct: 9   LVAFVLLLYMVSPLISPKPLALP--GAHVVVTGGSSGIGKCIAIECYKQGAFITLVARNE 68

Query: 74  KKLEEAKDAIR----------LSTGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGV 133
            KL +AK  I           L   +DV+   + V   + I +A ++ GP+D+L+   G+
Sbjct: 69  DKLLQAKKEIEKHSINDKQVVLCISVDVSQDYSQVE--NVIKQAQEKLGPVDMLVNCAGM 128

Query: 134 FVPQELAEQELDEVKFMLDVNLLGTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIY 193
            +  +  + E+   + ++ +N LG+    +A +  MK R   R   +  +SSQAGQ+G++
Sbjct: 129 SLSGKFEDLEVSTFERLMSINYLGSVYPSRAVIATMKER---RMGRVVFVSSQAGQLGLF 188

Query: 194 GYTAYSASKFGIRGLAEALQQEVIGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXX 253
           GYTAYS+SKF +RGLAEALQ EV   ++++++ +PPDTDTPGFA+E + +P  T      
Sbjct: 189 GYTAYSSSKFALRGLAEALQMEVKPYNVYVTVAYPPDTDTPGFAKENQTKPLETRLISET 248

Query: 254 XGSMKPELVAKKAFNGIKSGSFIIPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVR 309
               KPE VAK+    ++ G+F      +G +LS  T G++P  S +    +VV  G+ R
Sbjct: 249 TSVCKPEQVAKQIVKDVQ-GNFNSSIGSDGYMLSSLTCGMAPVTSIMEGLQQVVTMGLFR 306

[Close]

BLAST of Cla97C05G093370 vs. TAIR10
Match: AT3G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 358.2 bits (918), Expect = 5.3e-99
Identity = 181/299 (60.54%), Postives = 224/299 (74.92%), Query Frame = 0


Query: 27  VRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLST 86
           VRPR +KIP+K+RH FITGGSSGIGLALAH+AA EGARVSILARS  KLEEAK +I+L+T
Sbjct: 28  VRPRPIKIPIKSRHAFITGGSSGIGLALAHRAASEGARVSILARSGSKLEEAKKSIQLAT 87

Query: 87  GIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLG 146
           G++VA  +ADVRDYDA+SKA+DE+GPIDVLIVNQGVF  +EL +   ++VKF +DVNL+G
Sbjct: 88  GVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFTAKELVKHSPEDVKFTIDVNLVG 147

Query: 147 TFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVI 206
           +FN+IKAALPAM                                 FG++GLA+ALQQEVI
Sbjct: 148 SFNVIKAALPAMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFGLQGLAQALQQEVI 207

Query: 207 GDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFII 266
            DDIH++LIFPPDT+TPGF EEQK RPE+T       GSM+ E VAKKA +GIK+G+F +
Sbjct: 208 SDDIHVTLIFPPDTNTPGFEEEQKSRPEVTAIIAASSGSMETEEVAKKAMDGIKAGNFTV 267

Query: 267 PCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQRNR 326
            CNFEG LLS+AT G+SPQRSF +AFLEV+ AG +R++AL FQW WY +IEKW   + +
Sbjct: 268 SCNFEGFLLSLATTGMSPQRSFWLAFLEVITAGPIRLIALFFQWDWYKAIEKWSKTKTK 326

[Close]

BLAST of Cla97C05G093370 vs. TAIR10
Match: AT5G19200.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 355.9 bits (912), Expect = 2.6e-98
Identity = 185/300 (61.67%), Postives = 227/300 (75.67%), Query Frame = 0


Query: 26  IVRPRSVKIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLS 85
           IVRPRSV IP+K RHVFITGGSSGIGLALAH+A  EGA+VSILARS +KL EAK +I+L+
Sbjct: 25  IVRPRSVTIPIKFRHVFITGGSSGIGLALAHRAVSEGAKVSILARSTEKLAEAKRSIQLA 84

Query: 86  TGIDVAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLL 145
           TG++VA  +ADVRDYDA+SKA+DE+GPIDVLIVNQGVF+ +EL +Q  +EVKFM+DVNL 
Sbjct: 85  TGVEVATFSADVRDYDAVSKAIDESGPIDVLIVNQGVFIGKELEKQSPEEVKFMIDVNLT 144

Query: 146 GTFNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEV 205
           G+FN+IKAALPAMK R    PASI                     KFG++GLA+ALQQEV
Sbjct: 145 GSFNVIKAALPAMKAREGRGPASIXXXXXXXXXXXXXXXXXXXXXKFGLQGLAQALQQEV 204

Query: 206 IGDDIHISLIFPPDTDTPGFAEEQKKRPELTXXXXXXXGSMKPELVAKKAFNGIKSGSFI 265
           I D IH++L+FPPDTDTPGF +E KKRPELT       GSMK   VAK  F+GIK+G F 
Sbjct: 205 ISDGIHVTLLFPPDTDTPGFEQELKKRPELTSIIAASSGSMKTNEVAKICFDGIKAGKFT 264

Query: 266 IPCNFEGTLLSIATAGLSPQRSFLMAFLEVVGAGVVRIVALCFQWVWYGSIEKWHAQRNR 325
           + C+F G LLSIA+ G+SPQ SF +A  EV+  G++R+ +L FQW WY +IEKW +QRN+
Sbjct: 265 VTCHFIGFLLSIASTGMSPQGSFWLALTEVMFGGLIRLASLVFQWQWYKTIEKW-SQRNK 323

[Close]

BLAST of Cla97C05G093370 vs. TAIR10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 75.5 bits (184), Expect = 6.8e-14
Identity = 62/195 (31.79%), Postives = 97/195 (49.74%), Query Frame = 0


Query: 36  LKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLSTGIDVAIHAA 95
           L+ +   ITGG+SGIG A A +   +GA+V I+       EEA   +    G        
Sbjct: 36  LEGKVAVITGGASGIGKATAEEFVSQGAQVIIVDID----EEAGHMVATELGSAAHFLRC 95

Query: 96  DVRDYDAISKAVDEA----GPIDVLIVNQGV---FVPQELAEQELDEVKFMLDVNLLGTF 155
           DV + + I+KAV+ A    G +DV++ + G+     P  +A+ ++D    ++ +N+ GT 
Sbjct: 96  DVTEEEQIAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTV 155

Query: 156 NMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGD 215
             IK A  AM   +     SI  +SS +G +G  G  AYS SKF I G+ + +  E+   
Sbjct: 156 LGIKHAARAM---IPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTVASELCKH 215

Query: 216 DIHISLIFPPDTDTP 224
            + I+ I P    TP
Sbjct: 216 GLRINCISPAGIPTP 223

[Close]

BLAST of Cla97C05G093370 vs. TAIR10
Match: AT2G29370.1 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 75.1 bits (183), Expect = 8.9e-14
Identity = 59/196 (30.10%), Postives = 92/196 (46.94%), Query Frame = 0


Query: 33  KIPLKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEEAKDAIRLSTGIDVAI 92
           K  L+     +TGGS G+G A+  + A+ GARV   AR   +L+E+    + + G+ V  
Sbjct: 13  KWSLEGMTALVTGGSKGLGKAVVEELAMLGARVHTCARDETQLQESLREWQ-AKGLQVTT 72

Query: 93  HAADVRDYDAISKAVDEA-----GPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGT 152
              DV   D   K ++       G + +L+ N G+ V +   E   +E  F++  NL  T
Sbjct: 73  SVCDVSSRDQREKLMETVSSLFQGKLSILVPNVGIGVLKPTTECTAEEFSFIIATNLEST 132

Query: 153 FNMIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIG 212
           F+  + A P +K        +I LMSS AG V +   + Y A+K  +  LA  L  E   
Sbjct: 133 FHFSQLAHPLLK---ASGSGNIVLMSSVAGVVNLGNTSIYGATKGAMNQLARNLACEWAS 192

Query: 213 DDIHISLIFPPDTDTP 224
           D+I  + + P    TP
Sbjct: 193 DNIRANSVCPWFITTP 204

[Close]

BLAST of Cla97C05G093370 vs. TAIR10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 68.9 bits (167), Expect = 6.4e-12
Identity = 61/210 (29.05%), Postives = 100/210 (47.62%), Query Frame = 0


Query: 36  LKNRHVFITGGSSGIGLALAHQAALEGARVSILARSFKKLEE-----AKDAIRLSTGI-D 95
           L+  +  +TGG+ GIG A+  + ++ GARV   AR   +L+E      +   +++T I D
Sbjct: 7   LQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTSICD 66

Query: 96  VAIHAADVRDYDAISKAVDEAGPIDVLIVNQGVFVPQELAEQELDEVKFMLDVNLLGTFN 155
           V++     +  + +S      G +++L+ N G  + +   E   +E  F++  NL   F+
Sbjct: 67  VSLREQREKLMETVSSLFQ--GKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAFH 126

Query: 156 MIKAALPAMKNRVDHRPASIALMSSQAGQVGIYGYTAYSASKFGIRGLAEALQQEVIGDD 215
           + + A P +K        SI LMSS AG V +   + Y A+K  +  LA  L  E   D+
Sbjct: 127 ISQLAHPLLK---ASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDN 186

Query: 216 IHISLIFPPDTDTPGFA-----EEQKKRPE 235
           I  + I P    TP  +     EE KK  E
Sbjct: 187 IRTNAICPWLITTPLISDLLSVEEMKKEAE 211

The following BLAST results are available for this feature:

BLAST of Cla97C05G093370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004134147.1	6.2e-162	94.48	PREDICTED: 3-dehydrosphinganine reductase TSC10A [Cucumis sativus] >KGN57010.1 h...	[more]
XP_023539235.1	2.8e-154	87.12	3-dehydrosphinganine reductase TSC10A-like [Cucurbita pepo subsp. pepo]	[more]
XP_022973673.1	3.6e-154	86.81	3-dehydrosphinganine reductase TSC10A [Cucurbita maxima]	[more]
XP_022138240.1	7.5e-152	86.85	3-dehydrosphinganine reductase TSC10A-like [Momordica charantia]	[more]
XP_022933748.1	9.9e-152	86.46	3-dehydrosphinganine reductase TSC10A [Cucurbita moschata] >XP_022933757.1 3-deh...	[more]

BLAST of Cla97C05G093370 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A0A0L506\|A0A0A0L506_CUCSA	4.1e-162	94.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G149900 PE=4 SV=1	[more]
tr\|A0A1S4DT08\|A0A1S4DT08_CUCME	3.1e-146	96.52	3-dehydrosphinganine reductase TSC10A isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
tr\|A0A1S3AX60\|A0A1S3AX60_CUCME	3.1e-146	96.52	3-dehydrosphinganine reductase TSC10A isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
tr\|A0A2I4G9E7\|A0A2I4G9E7_9ROSI	8.9e-133	76.92	3-dehydrosphinganine reductase TSC10A-like isoform X1 OS=Juglans regia OX=51240 ...	[more]
tr\|A0A2I4G9D0\|A0A2I4G9D0_9ROSI	8.9e-133	77.06	3-dehydrosphinganine reductase TSC10A-like isoform X2 OS=Juglans regia OX=51240 ...	[more]

BLAST of Cla97C05G093370 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q0WRJ2\|TC10A_ARATH	9.5e-98	60.54	3-dehydrosphinganine reductase TSC10A OS=Arabidopsis thaliana OX=3702 GN=TSC10A ...	[more]
sp\|F4JZN6\|TC10B_ARATH	4.7e-97	61.67	3-dehydrosphinganine reductase TSC10B OS=Arabidopsis thaliana OX=3702 GN=TSC10B ...	[more]
sp\|Q06136\|KDSR_HUMAN	3.9e-43	34.86	3-ketodihydrosphingosine reductase OS=Homo sapiens OX=9606 GN=KDSR PE=1 SV=1	[more]
sp\|Q6GV12\|KDSR_MOUSE	1.7e-41	36.18	3-ketodihydrosphingosine reductase OS=Mus musculus OX=10090 GN=Kdsr PE=1 SV=1	[more]
sp\|Q2KIJ5\|KDSR_BOVIN	4.8e-41	34.64	3-ketodihydrosphingosine reductase OS=Bos taurus OX=9913 GN=KDSR PE=2 SV=1	[more]

BLAST of Cla97C05G093370 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G06060.1	5.3e-99	60.54	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G19200.1	2.6e-98	61.67	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G26760.1	6.8e-14	31.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29370.1	8.9e-14	30.10	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G29290.2	6.4e-12	29.05	NAD(P)-binding Rossmann-fold superfamily protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **INTERPRO**
Term	Definition
IPR036291	NAD(P)-bd_dom_sf
IPR020904	Sc_DH/Rdtase_CS
IPR002347	SDR_fam

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006687	glycosphingolipid metabolic process
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0047560	3-dehydrosphinganine reductase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C05G093370.1	Cla97C05G093370.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 187..206 score: 34.31 coord: 40..57 score: 44.59 coord: 111..122 score: 45.43
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 40..231 e-value: 8.2E-44 score: 149.3
None	No IPR available	GENE3D	G3DSA:3.40.50.720		coord: 31..275 e-value: 5.7E-57 score: 195.1
None	No IPR available	PANTHER	PTHR43550:SF3	3-KETODIHYDROSPHINGOSINE REDUCTASE	coord: 7..324
None	No IPR available	PANTHER	PTHR43550	FAMILY NOT NAMED	coord: 7..324
None	No IPR available	CDD	cd08939	KDSR-like_SDR_c	coord: 39..272 e-value: 6.24245E-89 score: 267.967
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 174..202
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	SSF51735	NAD(P)-binding Rossmann-fold domains	coord: 34..265

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla97C05G093370	Cla013185	Watermelon (97103) v1	wmwmbB206
Cla97C05G093370	ClCG05G011190	Watermelon (Charleston Gray)	wcgwmbB229

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C05G093370	Silver-seed gourd	carwmbB0181
Cla97C05G093370	Silver-seed gourd	carwmbB0535
Cla97C05G093370	Silver-seed gourd	carwmbB0795
Cla97C05G093370	Cucumber (Gy14) v2	cgybwmbB209
Cla97C05G093370	Cucumber (Gy14) v1	cgywmbB644
Cla97C05G093370	Cucurbita maxima (Rimu)	cmawmbB264
Cla97C05G093370	Cucurbita maxima (Rimu)	cmawmbB473
Cla97C05G093370	Cucurbita moschata (Rifu)	cmowmbB244
Cla97C05G093370	Cucurbita moschata (Rifu)	cmowmbB454
Cla97C05G093370	Wild cucumber (PI 183967)	cpiwmbB224
Cla97C05G093370	Cucumber (Chinese Long) v3	cucwmbB220
Cla97C05G093370	Cucumber (Chinese Long) v2	cuwmbB218
Cla97C05G093370	Bottle gourd (USVL1VR-Ls)	lsiwmbB329
Cla97C05G093370	Melon (DHL92) v3.6.1	medwmbB424
Cla97C05G093370	Melon (DHL92) v3.5.1	mewmbB434
Cla97C05G093370	Wax gourd	wgowmbB194