Cla97C02G041780 (gene) Watermelon (97103) v2

Overview
NameCla97C02G041780
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionKunitz trypsin inhibitor 4
LocationCla97Chr02 : 29656959 .. 29657731 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGTATATATTAAACTATAATACTAAACTCAATTGATTCATAAGTTATATCATATTTAATATTTTATATGTATTTGATATAACGTAAGCTGATAGATTATGATACATTTAATTCTCAACATTAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAA

mRNA sequence

ATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAA

Coding sequence (CDS)

ATGATGATGTTCTATAAGTCACTTGCCATCTTTAGTTGCCTATGCTTCTTCATGCCCATATCTTCTACTGCCCAATCTGATGGTCCTCCCGTGCTCGACACCGAAGGCCGACCTCTACAGCGCGACGTTGAGTACTTCATCAAGCCTGCCATCACTGATGTCGCTGGTAATCTTACCCTAATTACCCGAAAAGGTGATCAGTGCCCGTTTTACGTTGGGCAACTACCACTTCTTTCACAAGATATAGGCTATGGAGTCACCTTCAGGCCTTATCTAGAGGGTGAAGATACAATTAGAGAAGGTAGAGATTTGAAGGTCGTGTTTCAAGCTTTTTCAATTTGCATAACGTCGACTGAATGGAAAGTGGGGGAGGCAGATCCTACTACCGGAAGGAGGTTTGTGAAGCTCGGGAGTGACAACACTGCCACCGGGTACTTTAGGATCGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGATCTGCATTTCCTTTTGAGTTTGTGAGAGTTGAGACAGCTGCAAAAGACCTTTGGTCAATCTTTCAACAGAAAAAATAA

Protein sequence

MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK
Homology
[Close]
BLAST of Cla97C02G041780 vs. NCBI nr
Match: XP_004139193.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] >KGN60878.1 hypothetical protein Csa_2G021000 [Cucumis sativus])

HSP 1 Score: 376.3 bits (965), Expect = 6.9e-101
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 0 

Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

[Close]
BLAST of Cla97C02G041780 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 364.8 bits (935), Expect = 2.1e-97
Identity = 178/217 (82.03%), Postives = 192/217 (88.48%), Query Frame = 0 

Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL +IFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 216

[Close]
BLAST of Cla97C02G041780 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 344.4 bits (882), Expect = 2.9e-91
Identity = 164/199 (82.41%), Postives = 177/199 (88.94%), Query Frame = 0 

Query: 2   MMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61
           MM  KSL  FS LC  M +SS AQS  PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 62  TRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWK 121
           TRKGDQCPF+VGQ+PL SQ+IG+GVTF PYL GEDTIREGRDLKVVFQAFSICITST W+
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 122 VGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181
           VGE DPTTGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGSAFPFEFVR 201
           KGLRLLALDGSAFPFEFVR
Sbjct: 181 KGLRLLALDGSAFPFEFVR 197

[Close]
BLAST of Cla97C02G041780 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 163/199 (81.91%), Postives = 177/199 (88.94%), Query Frame = 0 

Query: 2   MMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61
           MM  KSL  FS LC  M +SS AQS  PPVLDTEGRPLQ +VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAITDVAGNLTLL 60

Query: 62  TRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWK 121
           TRKGDQCPF+VGQ+PL SQ+IG+GVTF PYL GEDTIREGRDLKVVFQAFSICITST W+
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 122 VGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181
           VGE DPTTGRRFV+ G ++TATGYFRID+ + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGSAFPFEFVR 201
           KGLRLLALDGSAFPFEFVR
Sbjct: 181 KGLRLLALDGSAFPFEFVR 197

[Close]
BLAST of Cla97C02G041780 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 6.0e-89
Identity = 162/199 (81.41%), Postives = 174/199 (87.44%), Query Frame = 0 

Query: 2   MMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61
           MM  KSL  FS LC  M  SS AQS  PPVLD EGRPL+ +VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 62  TRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWK 121
           TRKGDQCPF+VGQ+PL SQ+IG+GVTF PYL  ED IREGRDLKVVFQAFSICITST W+
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 122 VGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181
           VGEADPTTGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGSAFPFEFVR 201
           KGLRLLALDGSAFPFEFVR
Sbjct: 181 KGLRLLALDGSAFPFEFVR 197

[Close]
BLAST of Cla97C02G041780 vs. TrEMBL
Match: tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 4.5e-101
Identity = 180/217 (82.95%), Postives = 198/217 (91.24%), Query Frame = 0 

Query: 1   MMMFYKSL-AIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60
           MM F+KSL A+FS LCFFM I STAQ DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLT 60

Query: 61  LITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTE 120
           LITRKGDQCPFYVGQ+PLLSQ+IG+GVT  PYLEG+DTIREGRDLK VFQAF+ICIT T+
Sbjct: 61  LITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQ 120

Query: 121 WKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGIL 180
           WKVGE DP +GRRFVKLG+DNTATGYFRIDKS+LGVYNIGWCPSDVP +GRPRCGSAGIL
Sbjct: 121 WKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGIL 180

Query: 181 IEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQKK 217
           IEKG+R LALDG AFPFEFVRV+T  ++L SI ++KK
Sbjct: 181 IEKGVRFLALDGPAFPFEFVRVDT-GEELGSILKEKK 216

[Close]
BLAST of Cla97C02G041780 vs. TrEMBL
Match: tr|A0A1S3C0M7|A0A1S3C0M7_CUCME (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.4e-97
Identity = 178/217 (82.03%), Postives = 192/217 (88.48%), Query Frame = 0 

Query: 1   MMMFYKSL-AIFSCLCFFMPISSTA-QSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60
           MM F+KSL +IFS LCFFM I STA Q DGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 61  TLITRKGDQCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITST 120
           TLITRKGDQCPFYVGQ+PLLSQ+ G+ V+  PY EGEDTIREGRDLK VFQ F+ICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 121 EWKVGEADPTTGRRFVKLGSDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180
           +WKVGEADP TGRRFVKLG DNTATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 181 LIEKGLRLLALDGSAFPFEFVRVETAAKDLWSIFQQK 216
           LIEKG+R LALDG AFPFEFVRV+   ++L SI Q+K
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVD-PVEELGSILQEK 216

[Close]
BLAST of Cla97C02G041780 vs. TrEMBL
Match: tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 9.3e-54
Identity = 107/183 (58.47%), Postives = 130/183 (71.04%), Query Frame = 0 

Query: 18  MPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPL 77
           M I+STAQ   PPVLDT G+PLQR VEY+I PAITDV GNLTL +R    CP +VGQ P+
Sbjct: 1   MAITSTAQL--PPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPV 60

Query: 78  LSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG 137
            S +IG  VTFRP  EG D I EG  L +VFQA S C+TST+W+V   +  TGRRFV +G
Sbjct: 61  TSTNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIG 120

Query: 138 SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 197
            ++   G F I + D G YNI WCP+   + GRPRCG AGIL+E G+RL+ALDG AFPFE
Sbjct: 121 DEDGPAGIFGISR-DNGAYNIVWCPA---MMGRPRCGRAGILVENGVRLVALDGDAFPFE 177

Query: 198 FVR 201
           F++
Sbjct: 181 FIK 177

[Close]
BLAST of Cla97C02G041780 vs. TrEMBL
Match: tr|D1KFM3|D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides OX=3695 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.8e-53
Identity = 108/183 (59.02%), Postives = 123/183 (67.21%), Query Frame = 0 

Query: 20  ISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLS 79
           ++  A    PPVLDT G+PL+  VEY+I P ITDVAG LTL+ R G +CPFYVGQ PL S
Sbjct: 20  LAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQEPLAS 79

Query: 80  -QDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGS 139
            +  G  V F PY  GE  IRE RDL V FQA +ICI ST W+VGE DP TGRRF+    
Sbjct: 80  AEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRFIVTAG 139

Query: 140 DNTATGYFRIDKSDLGVYNIGWCPSD-VPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 199
           D +   YFRID +  GVYN  WCP++  P   RPRCGSAGILIE   RLLALDG AFPF 
Sbjct: 140 DKS---YFRIDNNG-GVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPAFPFV 198

Query: 200 FVR 201
           F R
Sbjct: 200 FTR 198

[Close]
BLAST of Cla97C02G041780 vs. TrEMBL
Match: tr|G0Z2L7|G0Z2L7_POPNI (Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.8e-53
Identity = 107/183 (58.47%), Postives = 123/183 (67.21%), Query Frame = 0 

Query: 20  ISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLS 79
           ++  A    PPVLDT G+PL+  VEY+I P ITD+AG LTL+ R G +CPFYVGQ PL S
Sbjct: 20  LAQPADDQSPPVLDTSGQPLETGVEYYILPGITDIAGGLTLVNRNGIRCPFYVGQEPLAS 79

Query: 80  -QDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLGS 139
            +  G  V F PY  GE  IRE RDL V FQA +ICI ST W+VGE DP TGRRF+    
Sbjct: 80  AEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRFIVTAG 139

Query: 140 DNTATGYFRIDKSDLGVYNIGWCPSD-VPIRGRPRCGSAGILIEKGLRLLALDGSAFPFE 199
           D +   YFRID +  GVYN  WCP++  P   RPRCGSAGILIE   RLLALDG AFPF 
Sbjct: 140 DKS---YFRIDNNG-GVYNFSWCPTESCPTCDRPRCGSAGILIEDDKRLLALDGPAFPFV 198

Query: 200 FVR 201
           F R
Sbjct: 200 FTR 198

[Close]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match: sp|P13087|MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-18
Identity = 72/215 (33.49%), Postives = 101/215 (46.98%), Query Frame = 0 

Query: 6   KSLAIFSCLCFFM---------PISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAG 65
           K L + S   FF+         P+ S A S   PVLD +G  L+    Y+I P + D  G
Sbjct: 2   KELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGG 61

Query: 66  NLTL--ITRKGD-QCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAFSI 125
            LT+   T  G   CP  V Q      D    + F P    ED +R   DL + F AF  
Sbjct: 62  GLTVSATTPNGTFVCPPRVVQ-TRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 121

Query: 126 C--ITSTEWKVGEADPTTGRRFVKLG------SDNTATGYFRIDK-SDLGVYNIGWCPSD 185
           C   +ST W++ + D +TG+ FV +G         T + +F+I++    G Y + +CP+ 
Sbjct: 122 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT- 181

Query: 186 VPIRGRPRCGSAGILI-EKGLRLLALDGSAFPFEF 199
           V    + +CG  GI I +KG R LAL    F FEF
Sbjct: 182 VCGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEF 214

[Close]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match: sp|P07596|IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 82.4 bits (202), Expect = 6.7e-15
Identity = 47/141 (33.33%), Postives = 70/141 (49.65%), Query Frame = 0 

Query: 17  FMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLP 76
           F P   +  +D PPV DT+G  L+ D  Y++  A     G LT+    G  CP +V Q P
Sbjct: 14  FWPAPPSRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP 73

Query: 77  LLSQDIGYGVTFRPY--LEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFV 136
               D G+ V   PY     +  IR   D+++ F+A++ C+ STEW + +++   GRR V
Sbjct: 74  NGQHD-GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHV 133

Query: 137 KLG-----SDNTATGYFRIDK 151
             G     S +     FRI+K
Sbjct: 134 ITGPVKDPSPSGRENAFRIEK 152

[Close]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match: sp|P29421|IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 79.0 bits (193), Expect = 7.4e-14
Identity = 59/178 (33.15%), Postives = 82/178 (46.07%), Query Frame = 0 

Query: 7   SLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGD 66
           SL +   L   + IS +  +  PPV DTEG  L  D  Y++ PA     G LT+  R   
Sbjct: 3   SLRLPLILLSLLAISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-L 62

Query: 67  QCPFYVGQLPLLSQDIGYGVTFRPY----LEGEDTIREGRDLKVVFQAFSICITSTEWKV 126
            CP  V Q     +  G+ V F P+       + TIR   D+++ F A +IC+ STEW V
Sbjct: 63  PCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHV 122

Query: 127 GEADPTTGRRFVKLG-----SDNTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGS 176
           G+ +P TG R V  G     S +     FR++K   G Y +  C       G  R G+
Sbjct: 123 GD-EPLTGARRVVTGPLIGPSPSGRENAFRVEKYG-GGYKLVSCRDSCQDLGVSRDGA 176

[Close]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match: sp|P16347|IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.8e-13
Identity = 44/131 (33.59%), Postives = 68/131 (51.91%), Query Frame = 0 

Query: 27  DGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQLPLLSQDIGYGV 86
           D PPV DT+G  L+ D  Y++ PA     G LT+    G +CP +V Q     +D G  V
Sbjct: 1   DPPPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRD-GLPV 60

Query: 87  TFRPY--LEGEDTIREGRDLKVVFQAFSICITSTEWKVGEADPTTGRRFVKLG-----SD 146
              P+     +  IR   D+++ F+A++ C+ STEW + +++  +GRR V  G     S 
Sbjct: 61  RIAPHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHI-DSELVSGRRHVITGPVRDPSP 120

Query: 147 NTATGYFRIDK 151
           +     FRI+K
Sbjct: 121 SGRENAFRIEK 129

[Close]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match: sp|P32765|ASP_THECC (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.5e-11
Identity = 49/165 (29.70%), Postives = 76/165 (46.06%), Query Frame = 0 

Query: 8   LAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDV-AGNLTLITRKGD 67
           L  F+   +F  +++ A S   PVLDT+G  LQ  V+Y++  +I+    G L L    G 
Sbjct: 11  LFAFTSKSYFFGVANAANS---PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQ 70

Query: 68  QCPFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQAF--SICITSTEWKVGE 127
            CP  V Q      D G  V F      +D +R   D+ + F      +C TST W++  
Sbjct: 71  SCPEIVVQ-RRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDN 130

Query: 128 ADPTTGRRFVKLG------SDNTATGYFRIDKSDLGVYNIGWCPS 164
            D + G+ +V           NT   +F+I+K+ +  Y   +CPS
Sbjct: 131 YDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPS 171

[Close]
BLAST of Cla97C02G041780 vs. TAIR10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 42.7 bits (99), Expect = 3.3e-04
Identity = 41/170 (24.12%), Postives = 69/170 (40.59%), Query Frame = 0 

Query: 1   MMMFYKSLAIFSCLCFFMPISSTAQSDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 60
           + + + +L + S +  F   S+   +    VLD  G P+Q +V+Y+I PA     G L  
Sbjct: 4   LTLSFITLTVLSAI--FTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIP 63

Query: 61  ITRK---GDQC---PFYVGQLPLLSQDIGYGVTFRPYLEGEDTIREGRDLKVVFQA-FSI 120
             R     D C          P +S   G  VTF P       ++    L + F +   +
Sbjct: 64  SNRNLSTQDLCLNLDIVQSSSPFVS---GLPVTFSPLNTKVKHVQLSASLNLEFDSTVWL 123

Query: 121 CITSTEWKVGEADPTTGRRFVKLGSD-NTATGYFRIDKSDLGVYNIGWCP 163
           C  S  W++ +      + FV +G        +F+I + D   Y + +CP
Sbjct: 124 CPDSKVWRI-DHSVQLRKSFVSIGGQKGKGNSWFQI-QEDGDAYKLMYCP 166

The following BLAST results are available for this feature:
BLAST of Cla97C02G041780 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004139193.16.9e-10182.95PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus] ... [more]
XP_008455014.12.1e-9782.03PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo][more]
XP_022938134.12.9e-9182.41alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata][more]
XP_023521354.11.1e-9081.91alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381... [more]
XP_022965952.16.0e-8981.41kunitz trypsin inhibitor 1-like [Cucurbita maxima][more]
BLAST of Cla97C02G041780 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LGB4|A0A0A0LGB4_CUCSA4.5e-10182.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=4 SV=1[more]
tr|A0A1S3C0M7|A0A1S3C0M7_CUCME1.4e-9782.03endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO... [more]
tr|A0A0A0LIK2|A0A0A0LIK2_CUCSA9.3e-5458.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021500 PE=4 SV=1[more]
tr|D1KFM3|D1KFM3_9ROSI7.8e-5359.02Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
tr|G0Z2L7|G0Z2L7_POPNI7.8e-5358.47Kunitz-type trypsin inhibitor C5 OS=Populus nigra OX=3691 PE=2 SV=1[more]
BLAST of Cla97C02G041780 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|P13087|MIRA_SYNDU2.9e-1833.49Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3[more]
sp|P07596|IAAS_HORVU6.7e-1533.33Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2[more]
sp|P29421|IAAS_ORYSJ7.4e-1433.15Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R... [more]
sp|P16347|IAAS_WHEAT2.8e-1333.59Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum OX=4565 PE=1 ... [more]
sp|P32765|ASP_THECC1.5e-1129.7021 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1[more]
BLAST of Cla97C02G041780 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G73325.13.3e-0424.12Kunitz family trypsin and protease inhibitor protein[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
Vocabulary: INTERPRO
TermDefinition
IPR011065Kunitz_inhibitor_STI-like_sf
IPR002160Prot_inh_Kunz-lg
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004866 endopeptidase inhibitor activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G041780.1Cla97C02G041780.1mRNA


InterPro
Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 30..202
e-value: 2.5E-29
score: 113.5
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 31..199
e-value: 2.0E-36
score: 125.5
IPR002160Proteinase inhibitor I3, Kunitz legumePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..200
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 31..47
IPR002160Proteinase inhibitor I3, Kunitz legumeCDDcd00178STIcoord: 30..200
e-value: 6.91964E-35
score: 122.451
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 28..204
e-value: 4.7E-39
score: 135.9
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..200
IPR011065Kunitz inhibitor STI-like superfamilySUPERFAMILYSSF50386STI-likecoord: 29..201

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cla97C02G041780Silver-seed gourdcarwmbB0746
Cla97C02G041780Silver-seed gourdcarwmbB0946
Cla97C02G041780Cucumber (Gy14) v2cgybwmbB114
Cla97C02G041780Cucumber (Gy14) v1cgywmbB100
Cla97C02G041780Cucumber (Chinese Long) v2cuwmbB118
Cla97C02G041780Cucurbita maxima (Rimu)cmawmbB175
Cla97C02G041780Cucurbita maxima (Rimu)cmawmbB810
Cla97C02G041780Cucurbita moschata (Rifu)cmowmbB161
Cla97C02G041780Cucurbita moschata (Rifu)cmowmbB785
Cla97C02G041780Wild cucumber (PI 183967)cpiwmbB120
Cla97C02G041780Cucumber (Chinese Long) v3cucwmbB123
Cla97C02G041780Bottle gourd (USVL1VR-Ls)lsiwmbB087
Cla97C02G041780Melon (DHL92) v3.6.1medwmbB367
Cla97C02G041780Melon (DHL92) v3.5.1mewmbB376
Cla97C02G041780Watermelon (Charleston Gray)wcgwmbB138
Cla97C02G041780Watermelon (97103) v1wmwmbB316
Cla97C02G041780Wax gourdwgowmbB244