Cla97C02G040710 (gene) Watermelon (97103) v2

Overview
NameCla97C02G040710
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionNicotianamine synthase
LocationCla97Chr02 : 28623957 .. 28625613 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTAACCGTTGTTGAATTAGTTATACCTATACTTATAGGTTTTATGGTTAGCTTTACATGCTTCTTGGATAAACATGAAATTTCATCTCGTAAAATTGATTAATCTATAATAACGAGAAAAATAACACATTTAGTGTATAAAAATTGTGAGGTTTCTCTATCTTTTAAAGAATGTGGGATCCTCAACGTGCCCCTTTGAGATGGTGTTTCTTTAGGTTTACCATTTTTGGGTTCAGATTTTTTTTTTTCCTTATGTTTCATAGACTTTGATACAAGATTAGATAAACATGAAGTTCTAACTCAAAATCAATTGGCAACAAGAGAGCAGTAACTCATTTATACTATGGGTCTCCTCATCTTTCCAATGTAAGATCCTCAATAAATATGTGATGTGTTAGATTTTTTTTGTAGATTTGATTGGTTGCTTTGTCATTTTCTTCACTTGTGTGGCGACAGTATACGAATTTGAATATCTAAACCATATCTTTCTTGTGTTCAAGGAGGAATTGGATAGGATGGAATGAAGTTATTATTAACTCTACTATTTGTTAGTGTAGTCCCATGACTTATTAACATAAACTCTATGACTTGTTAACTTCTTGCACTGTAGGCATTCCAGTATCGAACAATTTATCAAACTTAACAACTTCAAACTAAACAATTTCCGTTGTTAATAGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

mRNA sequence

ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Coding sequence (CDS)

ATGGAATTTGAAGAAGCCTCTCTCTTGCAAAAAGTCTGTGCTCTTTACAACCAAATCTCAAGCCTCGCTAGCCTCGAGCCTTCAAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCTCCACCTGCCCCTGGCCTTGATATTGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGGGCTCATCTCATTCGCCTTTGTGCTAAAGCTGAAGCTCTTCTTGAACTTCATTTCTCTTCCATTTTGGCTTCCTCTTTTGATAATCCCATTTCTAACTTGTCTATCTTCCCTTACTACTCTAATTACTTGAAGCTCTCCCTTCTTGAGTTTGATATTCTTCGATCTCATTATCGTCGTGTCCCCGCTACCGTCGCGTTCCTTGGCTCTGGACCCCTCCCGCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGAACTATTTTCCACAACTTTGACATTGATCCAGCTGCAAACACCATGGCTAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTCCACACAACAGATGTAATGGAAGTGACAAAGGGATTAAAAGATTATGAAGTAGTTTTCTTAGCAGCATTGGTAGGATTAGAAGAGGAAGAAAAAGGAAGAGTTCTAAGGCATTTGGGGAAACATATGGCAGCTGGTGCTTATTTGATGCTAAGAAGTGCACATGGAGCTAGAGCATTTTTATATCCTGTTGTTGATATTTGTAACGTTGAAGCTTCTGGATTTGAGATTTTATCTGTGTTCCATCCCACTGATGAAGTTATAAACTCTGTTGTTATTGCAAGGAAGAAACTAGTGTTTGATCGAAACTCGATCGAGGATGAGGATGACGACGAGGACGACGAGAAGCAAGGTATATTAGTTAATACCTCATCTATTGTGTTGCCTGAGAAATGCTCTGGGTTTAATGGCTTTAGTCCCATGATTGAAGAGCAACTTTCTTGA

Protein sequence

MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIEDEDDDEDDEKQGILVNTSSIVLPEKCSGFNGFSPMIEEQLS
Homology
[Close]
BLAST of Cla97C02G040710 vs. NCBI nr
Match: XP_004144750.1 (PREDICTED: probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical protein Csa_2G034530 [Cucumis sativus])

HSP 1 Score: 528.9 bits (1361), Expect = 1.2e-146
Identity = 291/332 (87.65%), Postives = 308/332 (92.77%), Query Frame = 0 

Query: 1   MEFE---EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAPGLDIASL 60
           MEFE   EA LL+KVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   PP  G DIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMRAHLI+LCA+AEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDL 180
           LRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAH 240
           SQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVL+HLGKHMAAG+YLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXX 300
           GARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK++FD++S   XXXXXXXX
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSS---XXXXXXXX 300

Query: 301 XXGILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           XXGILVNTSSIVL EKCSGFNGF+PMIEE LS
Sbjct: 301 XXGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of Cla97C02G040710 vs. NCBI nr
Match: XP_022946411.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 257/319 (80.56%), Postives = 278/319 (87.15%), Query Frame = 0 

Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           +VAF+GSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+FHTTD
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVFHTTD 181

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVT+   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 182 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 241

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNTSS 304
           D+ +VEASGFEILSVFHPTDEVINSVVIARK      ++     XX       +LVNT  
Sbjct: 242 DVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDXXTDDEKRAVLVNT-P 301

Query: 305 IVLPEKCSGFNGFSPMIEE 324
           I+L EKCSGF  F PMIEE
Sbjct: 302 IILAEKCSGFKDFGPMIEE 318

[Close]
BLAST of Cla97C02G040710 vs. NCBI nr
Match: XP_023545298.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.6 bits (1241), Expect = 1.0e-132
Identity = 259/319 (81.19%), Postives = 280/319 (87.77%), Query Frame = 0 

Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 61

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 62  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCRRVPS 121

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           +VAF+GSGPLPLSSIV+AS HLKGTIFHNFDIDPAANTMA QLVCSD DLS+RM+FHT D
Sbjct: 122 SVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANTMAGQLVCSDSDLSKRMVFHTAD 181

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVT+   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 182 VMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 241

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNTSS 304
           D+ +VEASGFEILSVFHPTDEVINSVVIARK      ++    XXXX      +LVN + 
Sbjct: 242 DVRDVEASGFEILSVFHPTDEVINSVVIARKVTDLVPDNDNYNXXXXDDEKRAVLVN-AP 301

Query: 305 IVLPEKCSGFNGFSPMIEE 324
           I+L EKCSGF  F PMIEE
Sbjct: 302 IILAEKCSGFKDFGPMIEE 318

[Close]
BLAST of Cla97C02G040710 vs. NCBI nr
Match: XP_008453482.1 (PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo])

HSP 1 Score: 481.1 bits (1237), Expect = 3.0e-132
Identity = 255/291 (87.63%), Postives = 267/291 (91.75%), Query Frame = 0 

Query: 1   MEFEE-------ASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GL 60
           MEFE+          LQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G 
Sbjct: 1   MEFEQXXXXXXXXXXLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPLQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVC 180
           LEFDILRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLM 240
           SDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+YLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFD 281
           LRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]
BLAST of Cla97C02G040710 vs. NCBI nr
Match: XP_022998973.1 (nicotianamine synthase-like, partial [Cucurbita maxima])

HSP 1 Score: 480.3 bits (1235), Expect = 5.1e-132
Identity = 255/321 (79.44%), Postives = 279/321 (86.92%), Query Frame = 0 

Query: 5   EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAH 64
           E  LL++VCALY+QISSLASL+PSKNVDTLFTQLVLTCSPPAPGLDIASL QPLQAMRAH
Sbjct: 32  EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCSPPAPGLDIASLPQPLQAMRAH 91

Query: 65  LIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPA 124
           LIRLCA AEALLE+HFSSILA SF+NPI NLSIFPYYSNYLKLSLLEFDIL SH RRVP+
Sbjct: 92  LIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILISHCRRVPS 151

Query: 125 TVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTD 184
           ++AF+GSGPLPLSSIV+AS HLKGTIFHNFDIDPAAN+MA +L+ SD DLS+RM+FHT D
Sbjct: 152 SIAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPAANSMARRLIRSDSDLSKRMVFHTAD 211

Query: 185 VMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVV 244
           VMEVTK   +YEVVFLAALVGLEEEEKGRVLRHLGKHMA GAYLMLRSAHGARAFLYPVV
Sbjct: 212 VMEVTKAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFLYPVV 271

Query: 245 DICNVEASGFEILSVFHPTDEVINSVVIARK--KLVFDRNSIXXXXXXXXXXXXGILVNT 304
           D+ ++EASGFEILSVFHPTDEVINSVVIARK   LV D N+             G+LVNT
Sbjct: 272 DVSDIEASGFEILSVFHPTDEVINSVVIARKVSDLVLDNNN--YNDNNTDDEKRGVLVNT 331

Query: 305 SSIVLPEKCSGFNGFSPMIEE 324
             I+L EKCSGF  F PMI+E
Sbjct: 332 -PIILAEKCSGFKDFGPMIDE 348

[Close]
BLAST of Cla97C02G040710 vs. TrEMBL
Match: tr|A0A0A0LGI4|A0A0A0LGI4_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 8.3e-147
Identity = 291/332 (87.65%), Postives = 308/332 (92.77%), Query Frame = 0 

Query: 1   MEFE---EASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAPGLDIASL 60
           MEFE   EA LL+KVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   PP  G DIASL
Sbjct: 1   MEFEQQQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPPPIGFDIASL 60

Query: 61  SQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDI 120
           SQPL+AMRAHLI+LCA+AEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSLLEFDI
Sbjct: 61  SQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDI 120

Query: 121 LRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDL 180
           LRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVCSDPDL
Sbjct: 121 LRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDL 180

Query: 181 SQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAH 240
           SQRMIFHT DVMEVTKGLKDYEVVFLAALVGL EEEKGRVL+HLGKHMAAG+YLMLRSAH
Sbjct: 181 SQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLMLRSAH 240

Query: 241 GARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXX 300
           GARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK++FD++S   XXXXXXXX
Sbjct: 241 GARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSS---XXXXXXXX 300

Query: 301 XXGILVNTSSIVLPEKCSGFNGFSPMIEEQLS 327
           XXGILVNTSSIVL EKCSGFNGF+PMIEE LS
Sbjct: 301 XXGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

[Close]
BLAST of Cla97C02G040710 vs. TrEMBL
Match: tr|A0A1S3BXI8|A0A1S3BXI8_CUCME (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.0e-132
Identity = 255/291 (87.63%), Postives = 267/291 (91.75%), Query Frame = 0 

Query: 1   MEFEE-------ASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GL 60
           MEFE+          LQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G 
Sbjct: 1   MEFEQXXXXXXXXXXLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPLQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVC 180
           LEFDILRSH RR+P  VAF+GSGPLPLSSIVLASIHLKGTIFHNFDIDP ANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLM 240
           SDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+YLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFD 281
           LRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

[Close]
BLAST of Cla97C02G040710 vs. TrEMBL
Match: tr|A0A1S3BWE4|A0A1S3BWE4_CUCME (probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=4 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 6.3e-131
Identity = 255/293 (87.03%), Postives = 267/293 (91.13%), Query Frame = 0 

Query: 1   MEFEE-------ASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCS---PPAP-GL 60
           MEFE+          LQKVCALYNQISSL+SL+P KNVDTLFTQLVLTCS   P AP G 
Sbjct: 1   MEFEQXXXXXXXXXXLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPLQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHYRRVPATVAFLGSGPLPLSSIVLASIHLKG--TIFHNFDIDPAANTMASQL 180
           LEFDILRSH RR+P  VAF+GSGPLPLSSIVLASIHLKG  TIFHNFDIDP ANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAY 240
           VCSDPDLSQRMIFHT DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVL+HLGKHMAAG+Y
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFD 281
           LMLRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK+VFD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

[Close]
BLAST of Cla97C02G040710 vs. TrEMBL
Match: tr|A0A151TW62|A0A151TW62_CAJCA (Nicotianamine synthase OS=Cajanus cajan OX=3821 GN=KK1_010551 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.4e-98
Identity = 197/335 (58.81%), Postives = 246/335 (73.43%), Query Frame = 0 

Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  EE  L++KVC LY QISSL SL+PSKNVD LFTQLVLTC PP+P +D+  L + +Q 
Sbjct: 1   MVCEEEMLIEKVCELYEQISSLESLKPSKNVDMLFTQLVLTCIPPSP-IDVTKLDKTVQE 60

Query: 61  MRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYR 120
           +R+HLIRLC +AE  LE H+S+IL  S+DNP+ +L+IFPYYSNYLKL LLEF IL  H  
Sbjct: 61  IRSHLIRLCGEAEGHLETHYSTIL-GSYDNPLDHLNIFPYYSNYLKLGLLEFTILSQHCT 120

Query: 121 RVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIF 180
            VP  +AF+GSGPLPL+SIVLAS HL  T FHN+D+DP AN+ A++LV SDPDLS+RM+F
Sbjct: 121 HVPTKIAFVGSGPLPLTSIVLASNHLPSTTFHNYDMDPLANSNATRLVSSDPDLSKRMVF 180

Query: 181 HTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFL 240
           HTTD+++V+ GLK+YEVV+LAALVG+++E K R++ HL K+MA GA LMLRSAHGARAFL
Sbjct: 181 HTTDILDVSNGLKEYEVVYLAALVGMDKEGKNRIIDHLAKNMAPGALLMLRSAHGARAFL 240

Query: 241 YPVVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILV 300
           YPVV+ C++   GFE+LSVFHPTDEVINSVVIARK                     G+  
Sbjct: 241 YPVVEPCDLR--GFEVLSVFHPTDEVINSVVIARK--------YPQPVPMHSLDQQGL-- 300

Query: 301 NTSSIVLPEKCSGFNGFSPM----------IEEQL 326
             +S++LP KCS    F+P+          IEEQL
Sbjct: 301 --ASMILPNKCSEIQAFNPLNHGNIIEELAIEEQL 319

[Close]
BLAST of Cla97C02G040710 vs. TrEMBL
Match: tr|M5VZ49|M5VZ49_PRUPE (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G313000 PE=4 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 7.1e-98
Identity = 191/315 (60.63%), Postives = 245/315 (77.78%), Query Frame = 0 

Query: 7   SLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLI 66
           +L+Q+VC LY QISSL SL+PSK+V+ LFTQLVLTC PP+P +D++ L Q +Q +R+ LI
Sbjct: 7   ALVQRVCELYEQISSLESLKPSKDVNMLFTQLVLTCMPPSP-IDVSKLCQGVQEIRSKLI 66

Query: 67  RLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPATV 126
           RLC +AE LLE HFS+IL  S+++P+ +L+IFPYYSNYLKLS LEF IL  H+  VP+ +
Sbjct: 67  RLCGEAEGLLENHFSTIL-GSYEHPLDHLTIFPYYSNYLKLSKLEFTILSQHFPHVPSKI 126

Query: 127 AFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDVM 186
           AF+GSGPLPL+SIVLAS HL  T FHN+DIDP+AN+ A  LV SDPDLS+RM+FHTTD+M
Sbjct: 127 AFVGSGPLPLTSIVLASNHLTTTSFHNYDIDPSANSKALGLVSSDPDLSKRMVFHTTDIM 186

Query: 187 EVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVDI 246
           +VT  LKDY+VVFLAALVG+++ EK +++ HL K+MA GA LMLRSAHGARAFLYPV+D 
Sbjct: 187 DVTNALKDYDVVFLAALVGMDKMEKLKIIDHLAKYMAPGATLMLRSAHGARAFLYPVIDP 246

Query: 247 CNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNTSSIV 306
           C+ +  GFE+LSVFHPTDEVINSVVIARK  +  ++S                +N + I+
Sbjct: 247 CD-DLRGFEVLSVFHPTDEVINSVVIARKYALPAQHSSPIDSQG---------LNAAPII 306

Query: 307 LPEKCSGFNGFSPMI 322
           LP KCSG   F+P++
Sbjct: 307 LPNKCSGIEAFNPLL 309

[Close]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match: sp|Q9C7X5|NAS4_ARATH (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 8.4e-94
Identity = 182/326 (55.83%), Postives = 243/326 (74.54%), Query Frame = 0 

Query: 4   EEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRA 63
           ++  L+ K+C LY +IS L +L+P ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRR-V 123
           +LI++C +AE  LE HFSSIL S  DNP+ +L++FPYY+NYLKLS LEFD+L  +    V
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHT 183
           P TVAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FHT
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 184

Query: 184 TDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYP 243
            D+M+VT+ LK ++VVFLAALVG++++EK +V+ HL KHM+ GA LMLRSAHG RAFLYP
Sbjct: 185 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 244

Query: 244 VVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNT 303
           +V+ C++E  GFE+LSV+HPTDEVINS+VI+R KL  D N +             +  N 
Sbjct: 245 IVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGV---VHDHIDQASDLACNC 304

Query: 304 SSI--VLPEKCSGFNGFSPMIEEQLS 327
           S I  ++ +K S    F+   EEQL+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match: sp|O80483|NAS3_ARATH (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 8.6e-91
Identity = 168/276 (60.87%), Postives = 218/276 (78.99%), Query Frame = 0 

Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SL+PS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYR 120
           +R +LI++C  AE  LE HFSSIL S  DNP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-VPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP +VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V+ HL KHMA GA LMLRSAHG RAF
Sbjct: 181 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 241 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match: sp|Q9XGI7|NAS_SOLLC (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 8.1e-89
Identity = 176/315 (55.87%), Postives = 230/315 (73.02%), Query Frame = 0 

Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++KVC LY QIS L +L PSK+V+ LFT LV TC PP P +D++ L Q +Q +R+HLI+
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-IDVSKLCQKIQEIRSHLIK 68

Query: 68  LCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPATVA 127
           LC +AE LLE HFS IL SS++NP+ +L IFPY+ NY+KLSLLE++IL  +   +P  +A
Sbjct: 69  LCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIPKKIA 128

Query: 128 FLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDVME 187
           F+GSGPLPL+S+VLA+ HLK T FHN+DID  AN MAS LV +DPD+S RM FHT DVM+
Sbjct: 129 FIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTADVMD 188

Query: 188 VTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVDIC 247
           VT  LKDY+VVFLAALVG+++E+K +V+ HL K+M+ GA LMLRSAHGARAFLYPV+D  
Sbjct: 189 VTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPVLDPR 248

Query: 248 NVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNTSSIVL 307
           ++   GFE+LSV+HPTDEVINSV+IARK  V                   +L    + VL
Sbjct: 249 DLR--GFEVLSVYHPTDEVINSVIIARKLPV---------------PSVPLLDGLGAYVL 304

Query: 308 PEK--CSGFNGFSPM 321
           P K  C+  + F+P+
Sbjct: 309 PSKCACAEIHAFNPL 304

[Close]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match: sp|Q9FKT9|NAS2_ARATH (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.2e-88
Identity = 164/276 (59.42%), Postives = 211/276 (76.45%), Query Frame = 0 

Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LYNQIS+L SL+PSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHY 120
            MR+HLI+LC +AE  LE HFS+IL S  DNP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP  VAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMI
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMI 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHTTDV+   +GL  Y+VVFLAALVG+++E K + + HL KHMA GA +MLRSAHG RAF
Sbjct: 181 FHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 241 LYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match: sp|Q9FF79|NAS1_ARATH (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 4.9e-86
Identity = 161/270 (59.63%), Postives = 206/270 (76.30%), Query Frame = 0 

Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++++  LY+QIS L SL+PSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   VP  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDV 187
           +AF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIFHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 188 MEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVD 247
           +  T+GL  Y+VVFLAALVG+++E K + + HL KHMA GA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 248 ICNVEASGFEILSVFHPTDEVINSVVIARK 276
             + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 248 --SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

[Close]
BLAST of Cla97C02G040710 vs. TAIR10
Match: AT1G56430.1 (nicotianamine synthase 4)

HSP 1 Score: 345.1 bits (884), Expect = 4.6e-95
Identity = 182/326 (55.83%), Postives = 243/326 (74.54%), Query Frame = 0 

Query: 4   EEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRA 63
           ++  L+ K+C LY +IS L +L+P ++VDTLF QLV TC PP P +D+  +S+ +Q MR+
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPNIDVTKMSENIQEMRS 64

Query: 64  HLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRR-V 123
           +LI++C +AE  LE HFSSIL S  DNP+ +L++FPYY+NYLKLS LEFD+L  +    V
Sbjct: 65  NLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNGFV 124

Query: 124 PATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHT 183
           P TVAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM FHT
Sbjct: 125 PRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFFHT 184

Query: 184 TDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYP 243
            D+M+VT+ LK ++VVFLAALVG++++EK +V+ HL KHM+ GA LMLRSAHG RAFLYP
Sbjct: 185 VDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAFLYP 244

Query: 244 VVDICNVEASGFEILSVFHPTDEVINSVVIARKKLVFDRNSIXXXXXXXXXXXXGILVNT 303
           +V+ C++E  GFE+LSV+HPTDEVINS+VI+R KL  D N +             +  N 
Sbjct: 245 IVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGV---VHDHIDQASDLACNC 304

Query: 304 SSI--VLPEKCSGFNGFSPMIEEQLS 327
           S I  ++ +K S    F+   EEQL+
Sbjct: 305 SKIHVIMNKKKSIIEEFAGANEEQLT 324

[Close]
BLAST of Cla97C02G040710 vs. TAIR10
Match: AT1G09240.1 (nicotianamine synthase 3)

HSP 1 Score: 335.1 bits (858), Expect = 4.8e-92
Identity = 168/276 (60.87%), Postives = 218/276 (78.99%), Query Frame = 0 

Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQA 60
           M  ++  L+Q +C LY +IS L SL+PS++V+ LF QLV TC PP P +D+  +   +Q 
Sbjct: 1   MGCQDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPNIDVTKMCDRVQE 60

Query: 61  MRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYR 120
           +R +LI++C  AE  LE HFSSIL S  DNP+ +L+IFPYY+NYLKL  LEFD+L  +  
Sbjct: 61  IRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLN 120

Query: 121 R-VPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP +VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM 
Sbjct: 121 GFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMF 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V+ HL KHMA GA LMLRSAHG RAF
Sbjct: 181 FHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+V+ C+++  GFE+LS++HPTD+VINSVVI++K
Sbjct: 241 LYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKK 274

[Close]
BLAST of Cla97C02G040710 vs. TAIR10
Match: AT5G56080.1 (nicotianamine synthase 2)

HSP 1 Score: 325.9 bits (834), Expect = 2.9e-89
Identity = 164/276 (59.42%), Postives = 211/276 (76.45%), Query Frame = 0 

Query: 1   MEFEEASLLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASL-SQPLQ 60
           M  E   +++++  LYNQIS+L SL+PSKNVDTLF QLV TC P    +D+  +  + ++
Sbjct: 1   MACENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCLPTDTNIDVTEIHDEKVK 60

Query: 61  AMRAHLIRLCAKAEALLELHFSSILASSFDNPISNLSIFPYYSNYLKLSLLEFDILRSHY 120
            MR+HLI+LC +AE  LE HFS+IL S  DNP+++L IFPYY+NYLKL  LEFD+L  H 
Sbjct: 61  DMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHT 120

Query: 121 RRVPATVAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMI 180
             VP  VAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMI
Sbjct: 121 THVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMI 180

Query: 181 FHTTDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAF 240
           FHTTDV+   +GL  Y+VVFLAALVG+++E K + + HL KHMA GA +MLRSAHG RAF
Sbjct: 181 FHTTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAF 240

Query: 241 LYPVVDICNVEASGFEILSVFHPTDEVINSVVIARK 276
           LYP+VD C+++  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 241 LYPIVDSCDLK--GFEVLTIYHPSDDVVNSVVIARK 274

[Close]
BLAST of Cla97C02G040710 vs. TAIR10
Match: AT5G04950.1 (nicotianamine synthase 1)

HSP 1 Score: 319.3 bits (817), Expect = 2.7e-87
Identity = 161/270 (59.63%), Postives = 206/270 (76.30%), Query Frame = 0 

Query: 8   LLQKVCALYNQISSLASLEPSKNVDTLFTQLVLTCSPPAPGLDIASLSQPLQAMRAHLIR 67
           +++++  LY+QIS L SL+PSKNVDTLF QLV TC P    +D+ ++ + ++ MRA+LI+
Sbjct: 8   VVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCLPTDTNIDVTNMCEEVKDMRANLIK 67

Query: 68  LCAKAEALLELHFSSILAS--SFDNPISNLSIFPYYSNYLKLSLLEFDILRSHYRRVPAT 127
           LC +AE  LE HFS+IL S     NP+ +L IFPYYSNYLKL  LEFD+L  H   VP  
Sbjct: 68  LCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQHSSHVPTK 127

Query: 128 VAFLGSGPLPLSSIVLASIHLKGTIFHNFDIDPAANTMASQLVCSDPDLSQRMIFHTTDV 187
           +AF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+RMIFHTTDV
Sbjct: 128 IAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKRMIFHTTDV 187

Query: 188 MEVTKGLKDYEVVFLAALVGLEEEEKGRVLRHLGKHMAAGAYLMLRSAHGARAFLYPVVD 247
           +  T+GL  Y+VVFLAALVG+++E K + + HL KHMA GA LMLRSAH  RAFLYP+VD
Sbjct: 188 LNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALRAFLYPIVD 247

Query: 248 ICNVEASGFEILSVFHPTDEVINSVVIARK 276
             + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 248 --SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

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BLAST of Cla97C02G040710 vs. TAIR10
Match: AT4G26483.1 (nicotianamine synthases)

HSP 1 Score: 56.6 bits (135), Expect = 3.3e-08
Identity = 30/56 (53.57%), Postives = 41/56 (73.21%), Query Frame = 0 

Query: 222 MAAGAYLMLRSAHGARAFLYPVVDICNVEASGFEILSVFHP--TDEVINSVVIARK 276
           M  GA LMLRSA G R+FLY  VD C+++  GFE+L ++HP  +D  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLK--GFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:
BLAST of Cla97C02G040710 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004144750.11.2e-14687.65PREDICTED: probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypot... [more]
XP_022946411.11.0e-13280.56nicotianamine synthase-like [Cucurbita moschata][more]
XP_023545298.11.0e-13281.19nicotianamine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_008453482.13.0e-13287.63PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo][more]
XP_022998973.15.1e-13279.44nicotianamine synthase-like, partial [Cucurbita maxima][more]
BLAST of Cla97C02G040710 vs. TrEMBL
Match NameE-valueIdentityDescription
tr|A0A0A0LGI4|A0A0A0LGI4_CUCSA8.3e-14787.65Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=4 SV=1[more]
tr|A0A1S3BXI8|A0A1S3BXI8_CUCME2.0e-13287.63probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
tr|A0A1S3BWE4|A0A1S3BWE4_CUCME6.3e-13187.03probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
tr|A0A151TW62|A0A151TW62_CAJCA5.4e-9858.81Nicotianamine synthase OS=Cajanus cajan OX=3821 GN=KK1_010551 PE=4 SV=1[more]
tr|M5VZ49|M5VZ49_PRUPE7.1e-9860.63Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_6G313000 PE=4 SV=1[more]
BLAST of Cla97C02G040710 vs. Swiss-Prot
Match NameE-valueIdentityDescription
sp|Q9C7X5|NAS4_ARATH8.4e-9455.83Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
sp|O80483|NAS3_ARATH8.6e-9160.87Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
sp|Q9XGI7|NAS_SOLLC8.1e-8955.87Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
sp|Q9FKT9|NAS2_ARATH5.2e-8859.42Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
sp|Q9FF79|NAS1_ARATH4.9e-8659.63Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
BLAST of Cla97C02G040710 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G56430.14.6e-9555.83nicotianamine synthase 4[more]
AT1G09240.14.8e-9260.87nicotianamine synthase 3[more]
AT5G56080.12.9e-8959.42nicotianamine synthase 2[more]
AT5G04950.12.7e-8759.63nicotianamine synthase 1[more]
AT4G26483.13.3e-0853.57nicotianamine synthases[more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0030418nicotianamine biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0030410nicotianamine synthase activity
Vocabulary: INTERPRO
TermDefinition
IPR004298Nicotian_synth
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0030410 nicotianamine synthase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C02G040710.1Cla97C02G040710.1mRNA


InterPro
Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 5..277
e-value: 6.0E-117
score: 389.8
IPR004298Nicotianamine synthasePANTHERPTHR32266FAMILY NOT NAMEDcoord: 4..276
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 1..280
score: 55.352
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 8..276
e-value: 2.0E-58
score: 199.8
NoneNo IPR availablePANTHERPTHR32266:SF12NICOTIANAMINE SYNTHASE-RELATEDcoord: 4..276

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cla97C02G040710Cla019969Watermelon (97103) v1wmwmbB316
Cla97C02G040710Csa2G034520Cucumber (Chinese Long) v2cuwmbB118
Cla97C02G040710MELO3C004184Melon (DHL92) v3.5.1mewmbB376
Cla97C02G040710MELO3C015257Melon (DHL92) v3.5.1mewmbB242
Cla97C02G040710ClCG02G014920Watermelon (Charleston Gray)wcgwmbB138
Cla97C02G040710CSPI02G04700Wild cucumber (PI 183967)cpiwmbB120
Cla97C02G040710Cucsa.090660Cucumber (Gy14) v1cgywmbB100
Cla97C02G040710CmaCh05G010740Cucurbita maxima (Rimu)cmawmbB810
Cla97C02G040710CmaCh12G010280Cucurbita maxima (Rimu)cmawmbB175
Cla97C02G040710CmoCh12G010340Cucurbita moschata (Rifu)cmowmbB161
Cla97C02G040710CmoCh05G010850Cucurbita moschata (Rifu)cmowmbB785
Cla97C02G040710Lsi11G006330Bottle gourd (USVL1VR-Ls)lsiwmbB087
Cla97C02G040710CsGy2G004840Cucumber (Gy14) v2cgybwmbB114
Cla97C02G040710MELO3C015257.2Melon (DHL92) v3.6.1medwmbB228
Cla97C02G040710MELO3C004184.2Melon (DHL92) v3.6.1medwmbB367
Cla97C02G040710Carg12001Silver-seed gourdcarwmbB0588
Cla97C02G040710Carg23258Silver-seed gourdcarwmbB0921
Cla97C02G040710CsaV3_2G007080Cucumber (Chinese Long) v3cucwmbB123
Cla97C02G040710Bhi06G001334Wax gourdwgowmbB130
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cla97C02G040710Watermelon (97103) v2wmbwmbB104
Cla97C02G040710Silver-seed gourdcarwmbB0068
Cla97C02G040710Cucurbita maxima (Rimu)cmawmbB363
Cla97C02G040710Cucurbita moschata (Rifu)cmowmbB347
Cla97C02G040710Bottle gourd (USVL1VR-Ls)lsiwmbB011
Cla97C02G040710Watermelon (Charleston Gray)wcgwmbB261
Cla97C02G040710Watermelon (97103) v1wmwmbB394