Cla97C02G034300 (gene) Watermelon (97103) v2 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla97C02G034300
Type	gene
Organism	Citrullus lanatus (Watermelon (97103) v2)
Description	Polygalacturonase
Location	Cla97Chr02 : 8513270 .. 8513804 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATGATCAAATCTAGCTTCTTGGTTACTACATTTTTGATGTCATTGTTATCATTTACTATTGAGTCTCGATCTCATGTTGATATCATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCTGGTGTTGATGTCACTAAGGTAAACACACAATAAAATGTATGCCAAAGTAATTACTTGTTGAATTGATCAATTGGTTTTGCTAATTTTTGTTACTTTGAAACATAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGGTCGTCCAAGCGCAATCATTATTCCAAGAGGCATATTTAAAGTAAGTGAAGGAAAATTTAAAGGTCCATGCAAGAGTCCTGTTGAAATTCGACTTCGAGGAACATTGCAGGCTCCAAAGCATCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTAATAGATTGACACTATCAGGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATCTTTGATTGCCATAAAAACAAAAATTGTGCCGACCTACCTATT

mRNA sequence

ATGGCTATGATCAAATCTAGCTTCTTGGTTACTACATTTTTGATGTCATTGTTATCATTTACTATTGAGTCTCGATCTCATGTTGATATCATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCTGGTGTTGATGTCACTAAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGGTCGTCCAAGCGCAATCATTATTCCAAGAGGCATATTTAAAGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATCTTTGATTGCCATAAAAACAAAAATTGTGCCGACCTACCTATT

Coding sequence (CDS)

ATGGCTATGATCAAATCTAGCTTCTTGGTTACTACATTTTTGATGTCATTGTTATCATTTACTATTGAGTCTCGATCTCATGTTGATATCATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCTGGTGTTGATGTCACTAAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGGTCGTCCAAGCGCAATCATTATTCCAAGAGGCATATTTAAAGTGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGGTTGGGAAAAGAATCTTTGATTGCCATAAAAACAAAAATTGTGCCGACCTACCTATT

Protein sequence

MAMIKSSFLVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPRGIFKVEEFLMVKERQVGKRIFDCHKNKNCADLPI

Homology

[Close]

BLAST of Cla97C02G034300 vs. NCBI nr
Match: XP_004149829.2 (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 1.9e-24
Identity = 60/78 (76.92%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 1  MAMIK-SSFLVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACA 60
          M +IK S+ LVTTFLMSLLSFTIESRSH+D+IFPL KYG+IV G DVT+ALKDAWNDACA
Sbjct: 1  MGVIKYSNLLVTTFLMSLLSFTIESRSHIDVIFPLEKYGNIVSGADVTEALKDAWNDACA 60

Query: 61 SGRPSAIIIPRGIFKVEE 78
          S  PSA++IP+GIFKV E
Sbjct: 61 SVHPSAVVIPKGIFKVRE 78

[Close]

BLAST of Cla97C02G034300 vs. NCBI nr
Match: XP_008455553.1 (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-23
Identity = 59/78 (75.64%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 1  MAMIK-SSFLVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACA 60
          M++IK SS LV TFLM+LLSFT+ESRSHVD+IF LRKYG+IV G DVT+ALKDAWNDACA
Sbjct: 1  MSVIKYSSLLVITFLMALLSFTVESRSHVDVIFHLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61 SGRPSAIIIPRGIFKVEE 78
          S RPSA++IP+G FKV E
Sbjct: 61 SLRPSAVVIPKGTFKVRE 78

[Close]

BLAST of Cla97C02G034300 vs. NCBI nr
Match: KGN43464.1 (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 112.8 bits (281), Expect = 6.8e-22
Identity = 56/69 (81.16%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 9  LVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIII 68
          LVTTFLMSLLSFTIE+RSHVD IF LRKYG+IV G DVT+ALKDAWNDACAS RPSA++I
Sbjct: 10 LVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVI 69

Query: 69 PRGIFKVEE 78
          P+G FKV E
Sbjct: 70 PKGTFKVRE 77

[Close]

BLAST of Cla97C02G034300 vs. NCBI nr
Match: XP_022932184.1 (polygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 107.8 bits (268), Expect = 2.2e-20
Identity = 46/71 (64.79%), Postives = 60/71 (84.51%), Query Frame = 0


Query: 20  FTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPRGIFKVEEFL 79
           FT++++S  D++FPL+KYG+IV G D+TKAL +AW DAC S R SAI+IP GIFKV+EFL
Sbjct: 109 FTVDAQSSADVVFPLKKYGNIVSGTDITKALANAWKDACTSNRSSAIVIPGGIFKVKEFL 168

Query: 80  MVKERQVGKRI 91
           M KERQ+GKR+
Sbjct: 169 MAKERQLGKRM 179

[Close]

BLAST of Cla97C02G034300 vs. NCBI nr
Match: XP_011659699.1 (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 103.6 bits (257), Expect = 4.1e-19
Identity = 50/63 (79.37%), Postives = 56/63 (88.89%), Query Frame = 0


Query: 15 MSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPRGIFK 74
          MSLLSFTIE+RSHVD IF LRKYG+IV G DVT+ALKDAWNDACAS RPSA++IP+G FK
Sbjct: 1  MSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVIPKGTFK 60

Query: 75 VEE 78
          V E
Sbjct: 61 VRE 62

[Close]

BLAST of Cla97C02G034300 vs. TrEMBL
Match: tr|A0A1S3C2F4|A0A1S3C2F4_CUCME (exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103495704 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.8e-23
Identity = 59/78 (75.64%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 1  MAMIK-SSFLVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACA 60
          M++IK SS LV TFLM+LLSFT+ESRSHVD+IF LRKYG+IV G DVT+ALKDAWNDACA
Sbjct: 1  MSVIKYSSLLVITFLMALLSFTVESRSHVDVIFHLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61 SGRPSAIIIPRGIFKVEE 78
          S RPSA++IP+G FKV E
Sbjct: 61 SLRPSAVVIPKGTFKVRE 78

[Close]

BLAST of Cla97C02G034300 vs. TrEMBL
Match: tr|A0A0A0K3B7|A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 4.5e-22
Identity = 56/69 (81.16%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 9  LVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIII 68
          LVTTFLMSLLSFTIE+RSHVD IF LRKYG+IV G DVT+ALKDAWNDACAS RPSA++I
Sbjct: 10 LVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVI 69

Query: 69 PRGIFKVEE 78
          P+G FKV E
Sbjct: 70 PKGTFKVRE 77

[Close]

BLAST of Cla97C02G034300 vs. TrEMBL
Match: tr|W9SC16|W9SC16_9ROSA (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_020041 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.5e-05
Identity = 30/72 (41.67%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 4  IKSSFLVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRP 63
          +K S  V   L+ L+S  I +   V  +F + + G I+P  D+T AL  AWN+ACAS  P
Sbjct: 3  LKLSNFVIAALLLLVSSNIANAQIV--LFDVTENGTILPQSDITDALLKAWNNACASTIP 62

Query: 64 SAIIIPRGIFKV 76
          S +++P+GIFK+
Sbjct: 63 SKVVVPKGIFKL 72

[Close]

BLAST of Cla97C02G034300 vs. TrEMBL
Match: tr|A0A151RS47|A0A151RS47_CAJCA (Polygalacturonase OS=Cajanus cajan OX=3821 GN=KK1_033140 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 8.5e-05
Identity = 27/64 (42.19%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 14 LMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPRGIF 73
          L+ LL+  + +++    +F +RKYG   P  D+TKAL+ AW DACAS  PS I++P G +
Sbjct: 12 LLLLLASNVRAQTG---LFDIRKYG-ATPNGDITKALQSAWKDACASMTPSKILVPSGKY 71

Query: 74 KVEE 78
          K+ +
Sbjct: 72 KLNQ 71

[Close]

BLAST of Cla97C02G034300 vs. TrEMBL
Match: tr|V7C552|V7C552_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_004G097300g PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.1e-04
Identity = 23/47 (48.94%), Postives = 35/47 (74.47%), Query Frame = 0


Query: 31 IFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPRGIFKVEE 78
          +F + KYG  VP  D+T+AL +AW+DACAS  PS ++I RGI+ +++
Sbjct: 27 VFDISKYG-AVPNGDITEALTNAWSDACASTTPSKVVIARGIYNLKQ 72

[Close]

BLAST of Cla97C02G034300 vs. Swiss-Prot
Match: sp|Q39766|PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.7e-04
Identity = 23/66 (34.85%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 9  LVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPG-VDVTKALKDAWNDACASGRPSAII 68
          +V +  + LL F   S+   D    + K+G    G  D++K   DAW +ACAS  PS ++
Sbjct: 7  IVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69 IPRGIF 74
          IP+G +
Sbjct: 67 IPKGTY 72

[Close]

BLAST of Cla97C02G034300 vs. Swiss-Prot
Match: sp|Q39786|PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.7e-04
Identity = 23/66 (34.85%), Postives = 36/66 (54.55%), Query Frame = 0


Query: 9  LVTTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPG-VDVTKALKDAWNDACASGRPSAII 68
          +V +  + LL F   S+   D    + K+G    G  D++K   DAW +ACAS  PS ++
Sbjct: 7  IVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69 IPRGIF 74
          IP+G +
Sbjct: 67 IPKGTY 72

[Close]

BLAST of Cla97C02G034300 vs. TAIR10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 18/38 (47.37%), Postives = 25/38 (65.79%), Query Frame = 0


Query: 43 GVDVTKALKDAWNDACASGRPSAIIIPRGIFKVEEFLM 81
          G D+T+AL  A+  AC S  PS ++IP+G FK+ E  M
Sbjct: 32 GSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEM 69

[Close]

BLAST of Cla97C02G034300 vs. TAIR10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 42.4 bits (98), Expect = 2.0e-04
Identity = 17/40 (42.50%), Postives = 27/40 (67.50%), Query Frame = 0


Query: 42 PGVDVTKALKDAWNDACASGRPSAIIIPRGIFKVEEFLMV 82
          PG D+T AL  A+N+AC     S ++IP+G +K+ E +M+
Sbjct: 32 PGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMM 71

[Close]

BLAST of Cla97C02G034300 vs. TAIR10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 23/70 (32.86%), Postives = 37/70 (52.86%), Query Frame = 0


Query: 11 TTFLMSLLSFTIESRSHVDIIFPLRKYGDIVPGVDVTKALKDAWNDACASGRPSAIIIPR 70
          T F++ LL F+  +   + +           PG D+T+AL  A+  AC S  P  ++IP+
Sbjct: 9  TIFVICLLGFSANAHEVLRLTSS--------PGSDITQALLRAFTTACQSPTPRKVVIPK 68

Query: 71 GIFKVEEFLM 81
          G FK+ E +M
Sbjct: 69 GQFKLGEIMM 70

[Close]

BLAST of Cla97C02G034300 vs. TAIR10
Match: AT2G43860.1 (Pectin lyase-like superfamily protein)

HSP 1 Score: 40.4 bits (93), Expect = 7.8e-04
Identity = 28/73 (38.36%), Postives = 39/73 (53.42%), Query Frame = 0


Query: 6  SSFLVTTFLMSLLSFT-IESRSH-VDIIFPLRKYGDIVPG-VDVTKALKDAWNDACASGR 65
          +S L+    + +L FT I S +H +     +  YG    G  D TKA   AW+ ACAS  
Sbjct: 5  TSCLILPLALIILDFTLISSLAHPIPSTLNVLSYGAKPDGSKDSTKAFLAAWDVACASAN 64

Query: 66 PSAIIIPRGIFKV 76
          P+ II+P+G F V
Sbjct: 65 PTTIIVPKGRFLV 77

The following BLAST results are available for this feature:

BLAST of Cla97C02G034300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149829.2	1.9e-24	76.92	PREDICTED: polygalacturonase-like [Cucumis sativus]	[more]
XP_008455553.1	2.8e-23	75.64	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
KGN43464.1	6.8e-22	81.16	hypothetical protein Csa_7G037910 [Cucumis sativus]	[more]
XP_022932184.1	2.2e-20	64.79	polygalacturonase-like [Cucurbita moschata]	[more]
XP_011659699.1	4.1e-19	79.37	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]

BLAST of Cla97C02G034300 vs. TrEMBL


Match Name	E-value	Identity	Description
tr\|A0A1S3C2F4\|A0A1S3C2F4_CUCME	1.8e-23	75.64	exopolygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103495704 PE=3 SV=1	[more]
tr\|A0A0A0K3B7\|A0A0A0K3B7_CUCSA	4.5e-22	81.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G037910 PE=3 SV=1	[more]
tr\|W9SC16\|W9SC16_9ROSA	8.5e-05	41.67	Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_020041 PE=3 SV=1	[more]
tr\|A0A151RS47\|A0A151RS47_CAJCA	8.5e-05	42.19	Polygalacturonase OS=Cajanus cajan OX=3821 GN=KK1_033140 PE=3 SV=1	[more]
tr\|V7C552\|V7C552_PHAVU	1.1e-04	48.94	Uncharacterized protein OS=Phaseolus vulgaris OX=3885 GN=PHAVU_004G097300g PE=3 ...	[more]

BLAST of Cla97C02G034300 vs. Swiss-Prot


Match Name	E-value	Identity	Description
sp\|Q39766\|PGLR_GOSBA	5.7e-04	34.85	Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1	[more]
sp\|Q39786\|PGLR_GOSHI	5.7e-04	34.85	Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1	[more]

BLAST of Cla97C02G034300 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G07820.1	2.0e-04	47.37	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	2.0e-04	42.50	Pectin lyase-like superfamily protein	[more]
AT3G07830.1	2.7e-04	32.86	Pectin lyase-like superfamily protein	[more]
AT2G43860.1	7.8e-04	38.36	Pectin lyase-like superfamily protein	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR011050	Pectin_lyase_fold/virulence

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016798	hydrolase activity, acting on glycosyl bonds
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla97C02G034300.1	Cla97C02G034300.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	SSF51126	Pectin lyase-like	coord: 43..80

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla97C02G034300	Silver-seed gourd	carwmbB1111
Cla97C02G034300	Cucumber (Gy14) v2	cgybwmbB116
Cla97C02G034300	Cucumber (Gy14) v1	cgywmbB459
Cla97C02G034300	Cucurbita maxima (Rimu)	cmawmbB571
Cla97C02G034300	Cucurbita maxima (Rimu)	cmawmbB617
Cla97C02G034300	Cucurbita moschata (Rifu)	cmowmbB547
Cla97C02G034300	Cucurbita moschata (Rifu)	cmowmbB591
Cla97C02G034300	Wild cucumber (PI 183967)	cpiwmbB121
Cla97C02G034300	Cucumber (Chinese Long) v3	cucwmbB125
Cla97C02G034300	Cucumber (Chinese Long) v2	cuwmbB119
Cla97C02G034300	Bottle gourd (USVL1VR-Ls)	lsiwmbB054
Cla97C02G034300	Melon (DHL92) v3.6.1	medwmbB087
Cla97C02G034300	Melon (DHL92) v3.5.1	mewmbB092
Cla97C02G034300	Watermelon (Charleston Gray)	wcgwmbB138
Cla97C02G034300	Watermelon (97103) v1	wmwmbB326
Cla97C02G034300	Wax gourd	wgowmbB370