Cla97C01G014090 (gene) Watermelon (97103) v2

NameCla97C01G014090
Typegene
OrganismCitrullus lanatus (Watermelon (97103) v2)
DescriptionProtein-lysine N-methyltransferase
LocationCla97Chr01 : 27847650 .. 27850690 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCACCAAGCAAATCGGAGAGCATTTGCGGAGACCCCATAATTGTTGAACAGGATGATGGTGATGATGATCCGCCATTGCTGAGCTCTCATGCTCTAGCTGCACTCAAGGAGTTCCTTGCCGAGCAAACCCAGCCCTCCACTGAACCTCACTTGGAAAATGGAGTTTCCGATGACTCAGAGGTCGCTTTAGTGGCGGAGGATTGGAGGCTGAGTCAATTCTGGTACGACCGCAACACGGCGGAGACAGTGGCTAATGAGGTTCTTACGCTATGCCAGAACTTTGATTCTCCTCGGGTCGCCTGTATTGCTTGCCCGACTCTCTACACCTATCTGAAGGTAATTGGATAGTCGTTTTCTTTTATCGCATTTTGGAACTTGAATTTTGTGTCTAATTTTCTGTTCTTTGGAGGTTCTGCTTTAGTTTACTTAGTTGAGGCTCGAATTTCAAGTGGGGAGTACCGATGATAAGAATTTGTTTCAGTCGCTTTATACCAACGTCTTATGATATACCTAACATTTGAACCTGAAATAAATATAAGTGCACGATGAATGATTTGTTGTTTCACTTTATAATTAAAAATTTATTGATGTGTTATCGATGAGAAGAAAATTTCGTTCATCGAAATAGGGATGATGAGGCATCTGTTAAGAGGCTGAAGGACCCTCTAATTGATTGGATATCGAAAGGGAAAATTTAGAAATTTACATATCTAAATAGGCATATCTCAAAGGAGGAAAAGAAACCTATTTACAAACTCAGGATCACTAAATTATGGCCCCCCGAAATACTCCGATGTTTGAGCTCCGAAGACATGGCTTCAACTAAATTCTCAAAAGTCTCTCTCCCATCCAGGAATATGATACCTTAGGTATGCAAAGCTGAAAGCTCAAAGTATGCCAGGATTTCTGAGCCAATGATTAGCATCATAAGTCATAACCATGTTTTCTAAGAATGATCAGTGCCATAAAATATCATGTTCCATGGGACTTCAAAGCCTTTTTAGAAATTAAGGCTATTTTCTTTCTAAATATTTGACAATGACAAACTGCCATAATCATTTGAAAGAGAAGCAATCTCTCAATTAATCAAATGACAAGCTTTCTCAATTGTCCTTGCAAATTTTGAAGGGATTTAAAACAAGGAGAGATAGTATAAGGGTGTATTTGCTGAGTGGCTTGAAGCGTTGAATTAATGTAGGTTTACCATCTTTAGAGAAATAGGACTTTATTTGTTACTGTTTCCCAAGTCACATTGTCTTAGTCCATTTGTAGTCAACCTTGATTAGAAACCTTTTTCTTTTGTGATCTCTCTCATTCACCATTTCAGGATTTGAGACTATTAATATTTTCTGGAAGGATTTGAGCTTTTTGCTATTTGCCTCCTAAAGTCCCAATTATTTGTAAGCATAAATTCCAATATGGAGACTATTAATTTATTGGGGCCAGATGAGACATTCGAGATTCTTACAACCAACCATGTGTGATCGCTAACCATCTTGTTAAGACAATGAATTTAGTTTAGTCAAAATCTAAAAGTGTGATAGAAGCTAGGAATATGTCTAAGTGGTTTAACCAGAAAGCTTTCTAAATCCCGTCATGAATTCATTGTACTGCAAATTTACAAAGTTTTTTATAATTTTTTTTTTCAAGTTGATTCAGAAATGTTAGAATTTAAATTTGAATTATAAATATTCTCTAACATGATTCAATTTGTTCTGATTTAGTCATGATAATATCTAATTTCTGGTGGCCTGGTTTGCACTTGTGCAGTGCACCAGTGCCTATAATTAAGCCTTAGACATTAGACTAGTCTATATGAGTTATGAATCTTTATTTTGGATTGGAAACCCTGTCCTTTCTTCCAAACCAGATTGTTATTTTCTCCACCTTTTTGTTTACACCAAAAGTAATTTCTTCTATCTTTCTGTAGCCCATTTTCTTGCAAAATTAGCCATCTTCTCTATTGACTAATATATTGCCTTAGTCTGATCTTCATTTGTCAGAAAATTGGTCCTCATGTATCTGCACAACTTCTTGAGTATGACAAACGCTTTTCACAACATGGTAGTGAATTCACATTTTACGATTACAATGAACCAGAAGAATTGGCGATGGAATTGAAGCACAATTTTCAGATTGTAGTAGCAGATCCTCCTTACCTGGTGAGAGATAATGTCTTTGTCCATAATTAGTTTTTTAACACTTTTGGATGCTTAATTTGTGAATCAGGCTTAAATATCCTTCCCATTCACAGGATTTTTTTTTTTTTTTTTTTGGCAAACACCTCTATTATTTGTGATTATTAGTGCATTCTTGTCAGAATGCTTTACACCAAAATCCATTCTTTACTCCCCTAGCTTTGACTGTCCCATCTAAATATGCAAGTTTCGTGTAACACAATATATGGGCTTACTGTACTCGTATAAAGATTCTGAATTCTGAACTCTGAACTATGGTGGCAAGTTCCTACTTTCATATCTTGGCAACAAACTAGAGTTTATCTAACTGGTTTATGATTCTGTTTTTTTATTGTATTAGCGCTTGGGATTATATATGTATGTGAAACAATGATTCTCTATCAAGCCATGAATAATCTGTGCTCCCTCTATGTTCCTTCTGGCAGAGCAAAGAATGCTTGGAGAAAGTCACTCGTACAATTTCTTTCCTAGCACAACATAGTGAATCTTACCTGCTATTACTAACAGGTATAGAAATGATGAATACCGCTTGTCCCAACATTATATTTCACATTGCAATTAGCTTTTATAACTGTAAATAAATATTCTATCAGTTGCCTAATTACCAAGATTTCTAAGATACTGGTTAGATTCGTCGATTTTATTCTATTGTTTTGCTGACGATTAAACACTATGCAACACCAAAATGCAGGAGAAGTGCAAGCAGAAATAGCAGCTGAGCTAATGGGTTTTCATCCATGTGGTTTTAGGCCACACCACTCTAGCAAGCTCGGAAACGAGTTCAGGCTGTTCACTAACTATAATCCCGGGACGAGATTAGGAGGATGGGAGCAATAG

mRNA sequence

ATGGAGTCACCAAGCAAATCGGAGAGCATTTGCGGAGACCCCATAATTGTTGAACAGGATGATGGTGATGATGATCCGCCATTGCTGAGCTCTCATGCTCTAGCTGCACTCAAGGAGTTCCTTGCCGAGCAAACCCAGCCCTCCACTGAACCTCACTTGGAAAATGGAGTTTCCGATGACTCAGAGGTCGCTTTAGTGGCGGAGGATTGGAGGCTGAGTCAATTCTGGTACGACCGCAACACGGCGGAGACAGTGGCTAATGAGGTTCTTACGCTATGCCAGAACTTTGATTCTCCTCGGGTCGCCTGTATTGCTTGCCCGACTCTCTACACCTATCTGAAGAAAATTGGTCCTCATGTATCTGCACAACTTCTTGAGTATGACAAACGCTTTTCACAACATGGTAGTGAATTCACATTTTACGATTACAATGAACCAGAAGAATTGGCGATGGAATTGAAGCACAATTTTCAGATTGTAGTAGCAGATCCTCCTTACCTGAGCAAAGAATGCTTGGAGAAAGTCACTCGTACAATTTCTTTCCTAGCACAACATAGTGAATCTTACCTGCTATTACTAACAGGAGAAGTGCAAGCAGAAATAGCAGCTGAGCTAATGGGTTTTCATCCATGTGGTTTTAGGCCACACCACTCTAGCAAGCTCGGAAACGAGTTCAGGCTGTTCACTAACTATAATCCCGGGACGAGATTAGGAGGATGGGAGCAATAG

Coding sequence (CDS)

ATGGAGTCACCAAGCAAATCGGAGAGCATTTGCGGAGACCCCATAATTGTTGAACAGGATGATGGTGATGATGATCCGCCATTGCTGAGCTCTCATGCTCTAGCTGCACTCAAGGAGTTCCTTGCCGAGCAAACCCAGCCCTCCACTGAACCTCACTTGGAAAATGGAGTTTCCGATGACTCAGAGGTCGCTTTAGTGGCGGAGGATTGGAGGCTGAGTCAATTCTGGTACGACCGCAACACGGCGGAGACAGTGGCTAATGAGGTTCTTACGCTATGCCAGAACTTTGATTCTCCTCGGGTCGCCTGTATTGCTTGCCCGACTCTCTACACCTATCTGAAGAAAATTGGTCCTCATGTATCTGCACAACTTCTTGAGTATGACAAACGCTTTTCACAACATGGTAGTGAATTCACATTTTACGATTACAATGAACCAGAAGAATTGGCGATGGAATTGAAGCACAATTTTCAGATTGTAGTAGCAGATCCTCCTTACCTGAGCAAAGAATGCTTGGAGAAAGTCACTCGTACAATTTCTTTCCTAGCACAACATAGTGAATCTTACCTGCTATTACTAACAGGAGAAGTGCAAGCAGAAATAGCAGCTGAGCTAATGGGTTTTCATCCATGTGGTTTTAGGCCACACCACTCTAGCAAGCTCGGAAACGAGTTCAGGCTGTTCACTAACTATAATCCCGGGACGAGATTAGGAGGATGGGAGCAATAG

Protein sequence

MESPSKSESICGDPIIVEQDDGDDDPPLLSSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGWEQ
BLAST of Cla97C01G014090 vs. NCBI nr
Match: XP_004143344.1 (PREDICTED: N(6)-adenine-specific DNA methyltransferase 2 [Cucumis sativus] >KGN48324.1 hypothetical protein Csa_6G476640 [Cucumis sativus])

HSP 1 Score: 446.8 bits (1148), Expect = 4.6e-122
Identity = 229/242 (94.63%), Postives = 235/242 (97.11%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KSESIC DP +VEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVS+D
Sbjct: 1   MESPTKSESICRDPTVVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALVAEDWRLSQFWYDR TAETVA+EVLTLCQNFDSPRVACIACPTLYTYLKKIGP+V
Sbjct: 61  SEVALVAEDWRLSQFWYDRVTAETVADEVLTLCQNFDSPRVACIACPTLYTYLKKIGPNV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYDKRFSQHGSEFTFYDYNEPEEL MELKHNFQI+VADPPYLSKECLEKVT TIS
Sbjct: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELPMELKHNFQIIVADPPYLSKECLEKVTHTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQHSESYLLLLTGEVQAE AAELMGFH CGFRPHHSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAQHSESYLLLLTGEVQAERAAELMGFHTCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 242

BLAST of Cla97C01G014090 vs. NCBI nr
Match: XP_008462634.1 (PREDICTED: protein-lysine N-methyltransferase N6AMT2 [Cucumis melo])

HSP 1 Score: 441.4 bits (1134), Expect = 1.9e-120
Identity = 215/242 (88.84%), Postives = 222/242 (91.74%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KSES+C DP +V            S HALAALKEFLAEQTQPSTEPHLENGVS+D
Sbjct: 1   MESPTKSESVCRDPRVVGEDDVDDDPPLLSFHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALVAEDWRLSQFWYDR+TAETVA+EVLTLCQ FDSPRVACIACPTLYTYLKKIGP+V
Sbjct: 61  SEVALVAEDWRLSQFWYDRHTAETVADEVLTLCQKFDSPRVACIACPTLYTYLKKIGPNV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYDKRFSQHGSEFTFYDYNEPEEL MELKHNFQIVVADPPYLSKECLEKV  TIS
Sbjct: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELPMELKHNFQIVVADPPYLSKECLEKVAHTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQHSESYLLLLTGEVQAE AAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAQHSESYLLLLTGEVQAERAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 242

BLAST of Cla97C01G014090 vs. NCBI nr
Match: XP_022132771.1 (EEF1A lysine methyltransferase 1 [Momordica charantia])

HSP 1 Score: 411.8 bits (1057), Expect = 1.6e-111
Identity = 211/242 (87.19%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KS +IC D  I E   XXXXXXX SSHALAALKEFLAEQ+QPS+E H ENGVS+D
Sbjct: 1   MESPTKSPTICTDLRIEE---XXXXXXXLSSHALAALKEFLAEQSQPSSELHSENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           S+VALVAEDWRLSQFWYDR+TAETVANEVLTLCQ FDSPRVAC+ACPTLYTYLKKIGPHV
Sbjct: 61  SQVALVAEDWRLSQFWYDRDTAETVANEVLTLCQKFDSPRVACVACPTLYTYLKKIGPHV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           S QLLEYDKRFSQHGS+FTFYDYNEPEEL  ELKH+FQIVVADPPYLSKECLEKVTRTIS
Sbjct: 121 SVQLLEYDKRFSQHGSDFTFYDYNEPEELPTELKHDFQIVVADPPYLSKECLEKVTRTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLA+H ES+LLLLTGEVQAE AAELMGFH CGFRP HSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAKHRESHLLLLTGEVQAERAAELMGFHLCGFRPRHSSKLGNEFRLFTNYNPGTRLGGW 239

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 239

BLAST of Cla97C01G014090 vs. NCBI nr
Match: XP_023543174.1 (EEF1A lysine methyltransferase 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 411.4 bits (1056), Expect = 2.2e-111
Identity = 209/242 (86.36%), Postives = 224/242 (92.56%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESPSKSE+ CGD  I E  XXXXXXXX SSHA+AALKEFL EQ Q   + HLE+GVS+D
Sbjct: 1   MESPSKSETTCGDLRIEE--XXXXXXXXLSSHAMAALKEFLDEQAQTQPDVHLESGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALV EDWRLSQFWYDR+TAETV NEVLTLCQNF+SPRVACIACPTLYTYLKK+GPHV
Sbjct: 61  SEVALVTEDWRLSQFWYDRDTAETVTNEVLTLCQNFESPRVACIACPTLYTYLKKLGPHV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYD+RFSQHGSEFTFYDYNEPEEL MELKH+FQIV+ADPPYLSKECLEKVTR+IS
Sbjct: 121 SAQLLEYDERFSQHGSEFTFYDYNEPEELPMELKHDFQIVIADPPYLSKECLEKVTRSIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQ+ ESYLLLLTGEVQAE AAELMGFHPCGFRPHHSSKLGNEFRLFTNY+PG+RLGGW
Sbjct: 181 FLAQNRESYLLLLTGEVQAERAAELMGFHPCGFRPHHSSKLGNEFRLFTNYDPGSRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 240

BLAST of Cla97C01G014090 vs. NCBI nr
Match: XP_022977235.1 (EEF1A lysine methyltransferase 1 [Cucurbita maxima])

HSP 1 Score: 409.8 bits (1052), Expect = 6.3e-111
Identity = 209/242 (86.36%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESPSKSE+ CGD  I E  XXXXXXXX SSHALAALKEFL EQ Q   + HLE+GVS+D
Sbjct: 1   MESPSKSETTCGDLRIEE--XXXXXXXXLSSHALAALKEFLDEQAQTQPDVHLESGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALV EDWRLSQFWYDR+TAETV NEVLTLCQNF+SPRVACIACPTLYTYLKK+GPHV
Sbjct: 61  SEVALVTEDWRLSQFWYDRDTAETVTNEVLTLCQNFESPRVACIACPTLYTYLKKLGPHV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYD+RFSQHGSEFTFYDYNEPEEL MELKH+FQIV+ADPPYLSKECLEKVTR+IS
Sbjct: 121 SAQLLEYDERFSQHGSEFTFYDYNEPEELPMELKHDFQIVIADPPYLSKECLEKVTRSIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQ+ ESYLLLLTGEVQA+ AAELMGFHPCGFRPHHSSKLGNEFRLFTNY+PG RLGGW
Sbjct: 181 FLAQNRESYLLLLTGEVQAKRAAELMGFHPCGFRPHHSSKLGNEFRLFTNYDPGPRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 240

BLAST of Cla97C01G014090 vs. TrEMBL
Match: tr|A0A0A0KHM5|A0A0A0KHM5_CUCSA (Protein-lysine N-methyltransferase Csa_6G476640 OS=Cucumis sativus OX=3659 GN=Csa_6G476640 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 3.1e-122
Identity = 229/242 (94.63%), Postives = 235/242 (97.11%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KSESIC DP +VEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVS+D
Sbjct: 1   MESPTKSESICRDPTVVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALVAEDWRLSQFWYDR TAETVA+EVLTLCQNFDSPRVACIACPTLYTYLKKIGP+V
Sbjct: 61  SEVALVAEDWRLSQFWYDRVTAETVADEVLTLCQNFDSPRVACIACPTLYTYLKKIGPNV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYDKRFSQHGSEFTFYDYNEPEEL MELKHNFQI+VADPPYLSKECLEKVT TIS
Sbjct: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELPMELKHNFQIIVADPPYLSKECLEKVTHTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQHSESYLLLLTGEVQAE AAELMGFH CGFRPHHSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAQHSESYLLLLTGEVQAERAAELMGFHTCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 242

BLAST of Cla97C01G014090 vs. TrEMBL
Match: tr|A0A1S3CHE6|A0A1S3CHE6_CUCME (Protein-lysine N-methyltransferase LOC103500945 OS=Cucumis melo OX=3656 GN=LOC103500945 PE=3 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 1.3e-120
Identity = 215/242 (88.84%), Postives = 222/242 (91.74%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KSES+C DP +V            S HALAALKEFLAEQTQPSTEPHLENGVS+D
Sbjct: 1   MESPTKSESVCRDPRVVGEDDVDDDPPLLSFHALAALKEFLAEQTQPSTEPHLENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           SEVALVAEDWRLSQFWYDR+TAETVA+EVLTLCQ FDSPRVACIACPTLYTYLKKIGP+V
Sbjct: 61  SEVALVAEDWRLSQFWYDRHTAETVADEVLTLCQKFDSPRVACIACPTLYTYLKKIGPNV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           SAQLLEYDKRFSQHGSEFTFYDYNEPEEL MELKHNFQIVVADPPYLSKECLEKV  TIS
Sbjct: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELPMELKHNFQIVVADPPYLSKECLEKVAHTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLAQHSESYLLLLTGEVQAE AAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAQHSESYLLLLTGEVQAERAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 242

BLAST of Cla97C01G014090 vs. TrEMBL
Match: tr|A0A0S2I9N1|A0A0S2I9N1_MOMCH (Protein-lysine N-methyltransferase OS=Momordica charantia OX=3673 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 1.6e-110
Identity = 209/242 (86.36%), Postives = 221/242 (91.32%), Query Frame = 0

Query: 1   MESPSKSESICGDPIIVEXXXXXXXXXXXSSHALAALKEFLAEQTQPSTEPHLENGVSDD 60
           MESP+KS +IC D  I E   XXXXXXX SSHALAALKEFLAEQ+QPS+E H ENGVS+D
Sbjct: 1   MESPTKSPTICTDLRIEE---XXXXXXXLSSHALAALKEFLAEQSQPSSELHSENGVSED 60

Query: 61  SEVALVAEDWRLSQFWYDRNTAETVANEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHV 120
           S+VALVAEDWRLSQFWYDR+TAETVANEVLTLCQ FDSPRVAC+ACPTLYTYLKKIGPHV
Sbjct: 61  SQVALVAEDWRLSQFWYDRDTAETVANEVLTLCQKFDSPRVACVACPTLYTYLKKIGPHV 120

Query: 121 SAQLLEYDKRFSQHGSEFTFYDYNEPEELAMELKHNFQIVVADPPYLSKECLEKVTRTIS 180
           S QLLEYDKRFSQHGS+FTFYDYNEPEEL  ELKH+FQIVVADPPYLSKECLEKVT TIS
Sbjct: 121 SVQLLEYDKRFSQHGSDFTFYDYNEPEELPTELKHDFQIVVADPPYLSKECLEKVTHTIS 180

Query: 181 FLAQHSESYLLLLTGEVQAEIAAELMGFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGW 240
           FLA+H ES+LLLL GEVQAE AAELMGFH CGFRP HSSKLGNEFRLFTNYNPGTRLGGW
Sbjct: 181 FLAKHRESHLLLLAGEVQAERAAELMGFHLCGFRPRHSSKLGNEFRLFTNYNPGTRLGGW 239

Query: 241 EQ 243
           EQ
Sbjct: 241 EQ 239

BLAST of Cla97C01G014090 vs. TrEMBL
Match: tr|A0A068UQK2|A0A068UQK2_COFCA (Protein-lysine N-methyltransferase GSCOC_T00031302001 OS=Coffea canephora OX=49390 GN=GSCOC_T00031302001 PE=3 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.1e-84
Identity = 154/213 (72.30%), Postives = 177/213 (83.10%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEV 89
           S++A+ ALKEFLAEQ   +T+    +   ++ EVALV EDWRLSQFWYDR TA+TVA EV
Sbjct: 32  STYAIEALKEFLAEQNHSTTQE--SSAEVEEEEVALVTEDWRLSQFWYDRETADTVAKEV 91

Query: 90  LTLCQNFDSPRVACIACPTLYTYLKKIGPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEEL 149
           LTLC +FDSP VAC+ACPTLY YLKKI   V AQLLEYDKRF Q+GSEFTFYDYN+PE++
Sbjct: 92  LTLCHSFDSPSVACVACPTLYAYLKKINSGVPAQLLEYDKRFEQYGSEFTFYDYNQPEDI 151

Query: 150 AMELKHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMGFH 209
              L H+F IVVADPPYLSKECLEKVT+TISFL +  +S+LLLLTGEVQ + AA L+G H
Sbjct: 152 PASLNHSFPIVVADPPYLSKECLEKVTQTISFLLRPGKSFLLLLTGEVQKDNAAALLGLH 211

Query: 210 PCGFRPHHSSKLGNEFRLFTNYNPGTRLGGWEQ 243
           PCGFRP HSSKLGNEFRLFTNY+PGTRLGGWEQ
Sbjct: 212 PCGFRPQHSSKLGNEFRLFTNYDPGTRLGGWEQ 242

BLAST of Cla97C01G014090 vs. TrEMBL
Match: tr|A0A072VIU1|A0A072VIU1_MEDTR (Protein-lysine N-methyltransferase MTR_1g054315 OS=Medicago truncatula OX=3880 GN=25483547 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.9e-84
Identity = 154/213 (72.30%), Postives = 177/213 (83.10%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEV 89
           SSHAL+ALKEFL+EQ     + H +    D  EV+LV+EDWRLSQFWYD  TA+TVA EV
Sbjct: 25  SSHALSALKEFLSEQ-----QSHSDAAGDDSEEVSLVSEDWRLSQFWYDSETAKTVAEEV 84

Query: 90  LTLCQNFDSPRVACIACPTLYTYLKKIGPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEEL 149
           LTLC N    RVACIACPTLY YLKK+ P+VSAQLLEYDKRF Q+GS+FTFYDYN PEEL
Sbjct: 85  LTLCSNGVDSRVACIACPTLYAYLKKMDPNVSAQLLEYDKRFEQYGSDFTFYDYNHPEEL 144

Query: 150 AMELKHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMGFH 209
             ELK++++++VADPPYLSKECLEKV  TISFL Q  ES+LLLLTGEVQ E AAE++G H
Sbjct: 145 PSELKNSYKVIVADPPYLSKECLEKVAETISFLRQPGESFLLLLTGEVQKESAAEILGLH 204

Query: 210 PCGFRPHHSSKLGNEFRLFTNYNPGTRLGGWEQ 243
           PCGFRPHHSSKLGNEFRLF+NY+P TRLGGWE+
Sbjct: 205 PCGFRPHHSSKLGNEFRLFSNYDPETRLGGWEK 232

BLAST of Cla97C01G014090 vs. Swiss-Prot
Match: sp|Q8WVE0|EFMT1_HUMAN (EEF1A lysine methyltransferase 1 OS=Homo sapiens OX=9606 GN=EEF1AKMT1 PE=1 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.9e-43
Identity = 91/211 (43.13%), Postives = 130/211 (61.61%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEV 89
           S+HALAAL+EF AEQ Q      +E G  D   + ++ E+W+LSQFWY + TA  +A E 
Sbjct: 13  SAHALAALQEFYAEQKQ-----QIEPGEDDKYNIGIIEENWQLSQFWYSQETALQLAQEA 72

Query: 90  LTLCQNFDSPRVACIACPTLYTYLKKI-GPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEE 149
           +      +  R+AC++ P++Y  L+++   + S  + EYDKRF+ +G EF FYDYN P +
Sbjct: 73  IAAVG--EGGRIACVSAPSVYQKLRELCRENFSIYIFEYDKRFAMYGEEFIFYDYNNPLD 132

Query: 150 LAMEL-KHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMG 209
           L   +  H+F IV+ADPPYLS+ECL K + T+ +L   +   +LL TG +  E AAEL+G
Sbjct: 133 LPERIAAHSFDIVIADPPYLSEECLRKTSETVKYL---TRGKILLCTGAIMEEQAAELLG 192

Query: 210 FHPCGFRPHHSSKLGNEFRLFTNYNPGTRLG 239
              C F P H+  L NEFR + NY+ G   G
Sbjct: 193 VKMCTFVPRHTRNLANEFRCYVNYDSGLDCG 213

BLAST of Cla97C01G014090 vs. Swiss-Prot
Match: sp|Q9CY45|EFMT1_MOUSE (EEF1A lysine methyltransferase 1 OS=Mus musculus OX=10090 GN=Eef1akmt1 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 7.2e-42
Identity = 94/207 (45.41%), Postives = 129/207 (62.32%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEV 89
           SSH LAAL+EF AEQ Q S  P  +    D   V ++ E+W+LSQFWY ++TA  +A E 
Sbjct: 13  SSHTLAALQEFYAEQKQ-SVNPRGD----DKYNVGVIEENWQLSQFWYSQDTALRLAREA 72

Query: 90  LTLCQNFDSPRVACIACPTLYTYLKKI-GPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEE 149
           +      +  R+AC++ P++Y  L+++     S  + EYD+RF+ +G EF FYDYN P E
Sbjct: 73  IDAAG--EGGRIACVSAPSVYQKLRELCREDSSVYIFEYDRRFAIYGDEFIFYDYNHPLE 132

Query: 150 LAMEL-KHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMG 209
           L   +  H+F +VVADPPYLS+ECL K + TI FL   +   +LL TG +  E AA+L+G
Sbjct: 133 LPERIAAHSFDLVVADPPYLSEECLRKTSETIQFL---TRGKILLCTGAIMEEQAAQLLG 192

Query: 210 FHPCGFRPHHSSKLGNEFRLFTNYNPG 235
              C F P HS  L NEFR +TNY+ G
Sbjct: 193 VKMCKFIPEHSRNLANEFRCYTNYDSG 209

BLAST of Cla97C01G014090 vs. Swiss-Prot
Match: sp|Q6GN98|EFMT1_XENLA (EEF1A lysine methyltransferase 1 OS=Xenopus laevis OX=8355 GN=eef1akmt1 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-41
Identity = 94/207 (45.41%), Postives = 130/207 (62.80%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSE---VALVAEDWRLSQFWYDRNTAETVA 89
           SSHALAAL+EF AEQ Q   +      +  D E   V  V EDW+LSQFWY   TA ++A
Sbjct: 14  SSHALAALQEFYAEQQQREAQK-----LGPDHEKFSVGSVEEDWQLSQFWYSDETALSLA 73

Query: 90  NEVLTLCQNFDSPRVACIACPTLYTYLKKI-GPHVSAQLLEYDKRFSQHGSEFTFYDYNE 149
            E + +C   ++ R+ACI+ P++Y  L+ + G  V  +LLEYD RF+ +G +F FYDYNE
Sbjct: 74  KEAIEVCG--ENGRIACISAPSIYQKLRGLAGESVYIRLLEYDNRFAVYGDDFVFYDYNE 133

Query: 150 PEELAMELK-HNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAE 209
           P +L   L+  +F IV+ADPPYLS+ECL+   +TI  L   S   ++L TG V  ++AA+
Sbjct: 134 PLKLPESLEPSSFDIVIADPPYLSEECLQNTAQTIKHL---SRGKIILCTGAVMEDLAAQ 193

Query: 210 LMGFHPCGFRPHHSSKLGNEFRLFTNY 232
            +G   C F P H+  L NEFR ++NY
Sbjct: 194 KLGLKKCKFIPEHTRNLANEFRCYSNY 210

BLAST of Cla97C01G014090 vs. Swiss-Prot
Match: sp|Q5ZKT6|EFMT1_CHICK (EEF1A lysine methyltransferase 1 OS=Gallus gallus OX=9031 GN=EEF1AKMT1 PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 5.2e-40
Identity = 94/207 (45.41%), Postives = 122/207 (58.94%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDSEVALVAEDWRLSQFWYDRNTAETVANEV 89
           SSH LAAL+EF  EQ Q   E    +   +   V  + EDW+LSQFWY   TA  +ANE 
Sbjct: 12  SSHTLAALQEFYLEQQQ--REGMKTSQGFNQYSVGSIEEDWQLSQFWYSDETASCLANEA 71

Query: 90  LTLCQNFDSPRVACIACPTLYTYLKKI-GPHVSAQLLEYDKRFSQHGSEFTFYDYNEPEE 149
           +         R+AC++ P++Y  L++  G   S  +LEYD+RFS +G EF FYDYN P  
Sbjct: 72  IVAAGK--GGRIACVSAPSVYQKLREQGGADFSVCILEYDRRFSVYGEEFIFYDYNNPLN 131

Query: 150 LAME-LKHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELMG 209
           L    L H+F IVVADPPYLS+ECL+K   TI +L   ++  +LL TG +  E AA+ +G
Sbjct: 132 LPEHLLPHSFDIVVADPPYLSEECLQKTAETIKYL---TKGKILLCTGAIMEEQAAKHLG 191

Query: 210 FHPCGFRPHHSSKLGNEFRLFTNYNPG 235
              C F P HS  L NEFR + NY  G
Sbjct: 192 VKICKFIPKHSRNLANEFRCYVNYASG 211

BLAST of Cla97C01G014090 vs. Swiss-Prot
Match: sp|Q17QF2|EFMT1_BOVIN (EEF1A lysine methyltransferase 1 OS=Bos taurus OX=9913 GN=EEF1AKMT1 PE=2 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 2.6e-39
Identity = 89/208 (42.79%), Postives = 127/208 (61.06%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQTQPSTEPHLENGVSDDS-EVALVAEDWRLSQFWYDRNTAETVANE 89
           SS+ALAAL+EF AEQ     + H  +   DD   + ++ E+W+LSQFWY   TA  +A +
Sbjct: 13  SSYALAALQEFYAEQ-----QHHHSDLCGDDKYNIGIIEENWQLSQFWYSPETATCLAED 72

Query: 90  VLTLCQNFDSPRVACIACPTLYTYLK-KIGPHVSAQLLEYDKRFSQHGSEFTFYDYNEPE 149
            +      +  R+AC++ P++Y  L+ +    VS  + EYD+RF+ +G +F +YDY  P 
Sbjct: 73  AVAAAG--EGGRIACVSAPSVYQKLRERHRDDVSVCIFEYDRRFAIYGEDFVYYDYKNPV 132

Query: 150 ELAMEL-KHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELM 209
           +L   +  H+F IVVADPPYLS+ECL K++ TI  L   +   +LL TG V  + AA+L+
Sbjct: 133 DLPERIATHSFDIVVADPPYLSEECLRKMSETIKLL---TRGKILLCTGAVMEDAAAKLL 192

Query: 210 GFHPCGFRPHHSSKLGNEFRLFTNYNPG 235
           G   C F P H+  LGNEFR F NYN G
Sbjct: 193 GVKMCKFIPEHTRTLGNEFRCFVNYNSG 210

BLAST of Cla97C01G014090 vs. TAIR10
Match: AT3G58470.1 (nucleic acid binding;methyltransferases)

HSP 1 Score: 282.7 bits (722), Expect = 2.1e-76
Identity = 143/216 (66.20%), Postives = 167/216 (77.31%), Query Frame = 0

Query: 30  SSHALAALKEFLAEQ--TQPSTEPHLE-NGVSDDSEVALVAEDWRLSQFWYDRNTAETVA 89
           SS ALAAL+EFLA+Q  T  ST P     G  +  +V LV EDWRLSQFWY+  TAETVA
Sbjct: 31  SSQALAALREFLADQNKTVASTPPASSVAGGEESDKVELVTEDWRLSQFWYEPETAETVA 90

Query: 90  NEVLTLCQNFDSPRVACIACPTLYTYLKKIGPHVSAQLLEYDKRFSQHGSEFTFYDYNEP 149
           +EV+TL Q     RVACIACPTLY YLKK  P +  QLLEYD RF ++G EFTFYDYNEP
Sbjct: 91  DEVVTLSQRIPGCRVACIACPTLYVYLKKRDPSLQVQLLEYDMRFERYGKEFTFYDYNEP 150

Query: 150 EELAMELKHNFQIVVADPPYLSKECLEKVTRTISFLAQHSESYLLLLTGEVQAEIAAELM 209
           E+L ++LKH F I+VADPPYLS+ECLE+V++TI FLA   +S LLLLTGEVQ E AAEL+
Sbjct: 151 EDLPLQLKHCFHIIVADPPYLSRECLERVSQTILFLASPVDSLLLLLTGEVQREHAAELL 210

Query: 210 GFHPCGFRPHHSSKLGNEFRLFTNYNPGTRLGGWEQ 243
           G  PC F+PHHSSKLGNEFRLF +Y+PGTRLGG E+
Sbjct: 211 GVRPCVFKPHHSSKLGNEFRLFISYDPGTRLGGLEE 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004143344.14.6e-12294.63PREDICTED: N(6)-adenine-specific DNA methyltransferase 2 [Cucumis sativus] >KGN4... [more]
XP_008462634.11.9e-12088.84PREDICTED: protein-lysine N-methyltransferase N6AMT2 [Cucumis melo][more]
XP_022132771.11.6e-11187.19EEF1A lysine methyltransferase 1 [Momordica charantia][more]
XP_023543174.12.2e-11186.36EEF1A lysine methyltransferase 1 [Cucurbita pepo subsp. pepo][more]
XP_022977235.16.3e-11186.36EEF1A lysine methyltransferase 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
tr|A0A0A0KHM5|A0A0A0KHM5_CUCSA3.1e-12294.63Protein-lysine N-methyltransferase Csa_6G476640 OS=Cucumis sativus OX=3659 GN=Cs... [more]
tr|A0A1S3CHE6|A0A1S3CHE6_CUCME1.3e-12088.84Protein-lysine N-methyltransferase LOC103500945 OS=Cucumis melo OX=3656 GN=LOC10... [more]
tr|A0A0S2I9N1|A0A0S2I9N1_MOMCH1.6e-11086.36Protein-lysine N-methyltransferase OS=Momordica charantia OX=3673 PE=2 SV=1[more]
tr|A0A068UQK2|A0A068UQK2_COFCA1.1e-8472.30Protein-lysine N-methyltransferase GSCOC_T00031302001 OS=Coffea canephora OX=493... [more]
tr|A0A072VIU1|A0A072VIU1_MEDTR1.9e-8472.30Protein-lysine N-methyltransferase MTR_1g054315 OS=Medicago truncatula OX=3880 G... [more]
Match NameE-valueIdentityDescription
sp|Q8WVE0|EFMT1_HUMAN2.9e-4343.13EEF1A lysine methyltransferase 1 OS=Homo sapiens OX=9606 GN=EEF1AKMT1 PE=1 SV=1[more]
sp|Q9CY45|EFMT1_MOUSE7.2e-4245.41EEF1A lysine methyltransferase 1 OS=Mus musculus OX=10090 GN=Eef1akmt1 PE=1 SV=1[more]
sp|Q6GN98|EFMT1_XENLA1.6e-4145.41EEF1A lysine methyltransferase 1 OS=Xenopus laevis OX=8355 GN=eef1akmt1 PE=2 SV=... [more]
sp|Q5ZKT6|EFMT1_CHICK5.2e-4045.41EEF1A lysine methyltransferase 1 OS=Gallus gallus OX=9031 GN=EEF1AKMT1 PE=2 SV=1[more]
sp|Q17QF2|EFMT1_BOVIN2.6e-3942.79EEF1A lysine methyltransferase 1 OS=Bos taurus OX=9913 GN=EEF1AKMT1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G58470.12.1e-7666.20nucleic acid binding;methyltransferases[more]
The following terms have been associated with this gene:
Vocabulary: Biological Process
TermDefinition
GO:0032259methylation
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0008168methyltransferase activity
Vocabulary: INTERPRO
TermDefinition
IPR019369Efm5/EEF1AKMT1
IPR002052DNA_methylase_N6_adenine_CS
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0018022 peptidyl-lysine methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005575 cellular_component
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016279 protein-lysine N-methyltransferase activity
molecular_function GO:0016740 transferase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla97C01G014090.1Cla97C01G014090.1mRNA


Analysis Name: InterPro Annotations of watermelon 97103 v2
Date Performed: 2019-05-12
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF10237N6-adenineMlasecoord: 68..232
e-value: 3.9E-48
score: 163.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availablePANTHERPTHR13200:SF0PROTEIN-LYSINE N-METHYLTRANSFERASE N6AMT2coord: 18..242
NoneNo IPR availablePANTHERPTHR13200UNCHARACTERIZEDcoord: 18..242
IPR002052DNA methylase, N-6 adenine-specific, conserved sitePROSITEPS00092N6_MTASEcoord: 160..166
IPR019369Protein-lysine N-methyltransferase Efm5/EEF1AKMT1HAMAPMF_03187Methyltr_EFM5coord: 21..237
score: 35.535