Cla023434 (gene) Watermelon (97103) v1

BLAST of Cla023434 vs. Swiss-Prot
Match: TRM51_VITVI (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Vitis vinifera GN=VIT_19s0014g03930 PE=3 SV=1)

HSP 1 Score: 814.7 bits (2103), Expect = 7.0e-235
Identity = 422/627 (67.30%), Postives = 498/627 (79.43%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHF---LPICFTSPFSSTTIHSQSQFSDQSFLYGP 60
           MVTKLFLRP SL  T  +   +FP       + +C  S  ++T I + +Q  D +  YGP
Sbjct: 1   MVTKLFLRPHSLSFTLLSGIHLFPKTSLSKPITLCLLSTTATTPILTLTQTLDPNLSYGP 60

Query: 61  SLCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAA 120
           SL KG           P+NH    L  +   P  E        E + D++AFTR+F++ A
Sbjct: 61  SLHKGT---------KPLNHQNHQL--IAATPGEE--------ECVFDKEAFTRVFNLTA 120

Query: 121 LRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQ-NWSNSDDGDGIG 180
           +RVPS+ CF LENRLRGHLLNWPRIRN+ARVPGDEVE  LV+ +GE+ N S+  + +G  
Sbjct: 121 IRVPSKDCFALENRLRGHLLNWPRIRNVARVPGDEVEDGLVKLLGEKRNSSDGSESEG-D 180

Query: 181 IDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGR 240
            DSL+RRIYGKAEGDGE LSPVLYR+ L KTFDS+G+ NFR+LAK+SRPKK+K +KEE R
Sbjct: 181 FDSLNRRIYGKAEGDGEILSPVLYRDTLAKTFDSQGFANFRNLAKLSRPKKKKRRKEEER 240

Query: 241 DEGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGIE 300
            EG +R GK++ A VEVVE  E E +D++GLLG+EFK  +WRGSTRLLLLDERYADKG+E
Sbjct: 241 SEGKKRTGKNEFAMVEVVEDGE-EGEDLRGLLGEEFKRKRWRGSTRLLLLDERYADKGVE 300

Query: 301 ELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVG 360
           ELPEAIKA+LKEDT + M  T ELV+C+LTLFY+YWQMNE+LEALLP+ MI+PSAFE VG
Sbjct: 301 ELPEAIKAVLKEDTGQSMTSTFELVKCKLTLFYNYWQMNEILEALLPEGMIVPSAFEMVG 360

Query: 361 HIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVT 420
           HIAHLNLRDEHLPY KLIAKVVLDKNKPKIQTVVNK D I+NDYRTMQLEVLAGN SLVT
Sbjct: 361 HIAHLNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKTDAIHNDYRTMQLEVLAGNRSLVT 420

Query: 421 TVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKV 480
           TV+ENG+RF +DLATVYWNSRLATER RL++ FT NDVVCDVF+GVGPI+ISAA+ VK+V
Sbjct: 421 TVIENGMRFQVDLATVYWNSRLATERQRLLNCFTRNDVVCDVFSGVGPIAISAAKKVKRV 480

Query: 481 YANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDA 540
           YANDLNPYA+EYLE NSV NKLERKI+VFNM+GRRFI+A+F S+KA  ITQVVMNLPNDA
Sbjct: 481 YANDLNPYAIEYLESNSVLNKLERKIKVFNMDGRRFINAMFTSDKAESITQVVMNLPNDA 540

Query: 541 AEYLDAFKGILRDRS-DIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRV 600
           AE+LDAF+GI R +S D +  LPMIHVYGFSK +DPEFDFH+RIRIAL+EVAVDV+M RV
Sbjct: 541 AEFLDAFRGIFRKKSRDKQLKLPMIHVYGFSKAQDPEFDFHQRIRIALSEVAVDVEMHRV 600

Query: 601 RLVAPGKWMLCASFRLPRTVALSKPTL 623
           RLVAPGKWML ASF LP++V  +K  L
Sbjct: 601 RLVAPGKWMLRASFILPKSVVFAKAVL 606

BLAST of Cla023434 vs. Swiss-Prot
Match: TRM52_ARATH (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Arabidopsis thaliana GN=At4g27340 PE=2 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 1.3e-209
Identity = 387/635 (60.94%), Postives = 482/635 (75.91%), Query Frame = 1

Query: 1   MVTKLFL-RPQSLP---LTAFTAKFI---FPGKHFLPIC-FTSPFSSTTIHSQSQFSDQS 60
           MV+KL L R  SLP   +++++A+FI   +P    L  C F+S  SSTT           
Sbjct: 1   MVSKLSLFRANSLPFPVISSYSARFILKPYPKPKTLIFCVFSSNLSSTT----------- 60

Query: 61  FLYGPSLCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRI 120
           F YGPSL KG  PV    R + +   R   +  IG            ++ LL+ED FTR+
Sbjct: 61  FPYGPSLLKGKKPVLDDIRLASIGRDRDAHRGKIGE-----FDESIEKDVLLNEDEFTRV 120

Query: 121 FDIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDG 180
           F+I+A+RVP++ CF LENRLRGHLLNWPRIRNIARVPGDE+E ++V+ +G +     +D 
Sbjct: 121 FEISAIRVPAKDCFALENRLRGHLLNWPRIRNIARVPGDEIEEDVVKLLGRETDEEEEDE 180

Query: 181 DGIGIDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRK--E 240
           D + +DS++RRI GKAEGDGE LS VL+R+KL +TF+S GY+ FR+LAK+SRPK+++  E
Sbjct: 181 DSV-VDSVNRRIRGKAEGDGERLSSVLHRDKLARTFNSTGYLKFRNLAKISRPKRKRKTE 240

Query: 241 KKEEGRDEGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKG-GKWRGSTRLLLLDER 300
           +  EG+++      ++++A VEVVE+   E +D +GLLG+ +   G+WRGSTRLLLLDE+
Sbjct: 241 RTREGKEKEIASR-RNEMAVVEVVETRGGE-EDFEGLLGEGYGSRGRWRGSTRLLLLDEK 300

Query: 301 YADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIP 360
           Y+ + +++LPEAIK L  E        + ELV+CRLTLFYDYW M E+LEA+LPK MI+P
Sbjct: 301 YSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAVLPKGMIVP 360

Query: 361 SAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLA 420
           SAFE VGHIAHLNLRDEHLPY +LIAKVVLDKN+PKIQTVVNKID I+ND+RTMQLEVLA
Sbjct: 361 SAFEMVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQTVVNKIDPIHNDFRTMQLEVLA 420

Query: 421 GNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISA 480
           GNHSLVT VVENGLRFH+DLA VYWNS+L TER RL+ GF  NDVVCDVFAGVGPI+++A
Sbjct: 421 GNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLGFDQNDVVCDVFAGVGPIALAA 480

Query: 481 ARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVV 540
           ARIVK+VYANDLNP+AVE++E+NSV NKLE++IE+FNM+GRRFI A+F+SEK  K+TQVV
Sbjct: 481 ARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMDGRRFIKAMFSSEKGQKVTQVV 540

Query: 541 MNLPNDAAEYLDAFKGILRDR-SDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAV 600
           MNLP DAAE LDAF+G+  DR  D   + P IHVYGFSK  DPEFDFHERIRIAL+EVAV
Sbjct: 541 MNLPKDAAESLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAV 600

Query: 601 DVDMRRVRLVAPGKWMLCASFRLPRTVALSKPTLN 624
           DV MR+VRLVAPGKWMLCASF LP+ VA S+  L+
Sbjct: 601 DVKMRKVRLVAPGKWMLCASFILPKNVAFSRKNLS 616

BLAST of Cla023434 vs. Swiss-Prot
Match: TRM5_XENTR (tRNA (guanine(37)-N1)-methyltransferase OS=Xenopus tropicalis GN=trmt5 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 4.8e-66
Identity = 144/349 (41.26%), Postives = 199/349 (57.02%), Query Frame = 1

Query: 282 RLLLLDERYADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEAL 341
           +L+LLD  Y  K I+   E+   L K+          ++ +  L L Y+ ++  E+L A+
Sbjct: 125 KLVLLDP-YKVKSIDSFDESDHVLFKQ-----FDVNPQVSQYELQLTYENFKCEEILRAV 184

Query: 342 LPKDMIIPSAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYR 401
           LPK   + S F  VGHIAH+NLRD  LPY  +I +V+LDKN P I +VVNK + I++ YR
Sbjct: 185 LPKGQDVTSGFSRVGHIAHMNLRDHQLPYKNVIGQVILDKN-PGITSVVNKTNTIDSTYR 244

Query: 402 TMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAG 461
             Q+EVLAG  +++T V EN + +  D + VYWN RL TE +R+I      DV+ DVFAG
Sbjct: 245 NFQMEVLAGEENMITKVKENYVTYEFDFSKVYWNPRLGTEHNRIIGFLKARDVLFDVFAG 304

Query: 462 VGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIF---- 521
           VGP ++ AA+    VYANDLNP + ++L  N   NK+E++++ FN +GR FI        
Sbjct: 305 VGPFAVPAAKKNCTVYANDLNPESYKWLLHNCKLNKVEKRVQAFNTDGRDFIKTTIKKEL 364

Query: 522 -------ASEKAPKITQVVMNLPNDAAEYLDAFKGILRDRSDIEFTLPMIHVYGFSKTRD 581
                  ++E+ P +  + MNLP  A E+LDAFK +L +     F LP IH Y FSK  D
Sbjct: 365 LKYADMPSAEEKPSL-HIAMNLPALAVEFLDAFKNLLEEEPCNSFILPTIHCYSFSKDDD 424

Query: 582 PEFDFHERIRIALTEVAVDVDMRRVRLVAPGKWMLCASFRLPRTVALSK 620
           P  D   R    L     D  M  VR VAP K M+C SF+LP +V   +
Sbjct: 425 PLQDVKARAESFLGTTLEDCSMHLVRNVAPNKEMVCISFKLPSSVLFQR 465

BLAST of Cla023434 vs. Swiss-Prot
Match: TRM5_DANRE (tRNA (guanine(37)-N1)-methyltransferase OS=Danio rerio GN=trmt5 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.5e-64
Identity = 151/344 (43.90%), Postives = 199/344 (57.85%), Query Frame = 1

Query: 282 RLLLLDERY---ADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVL 341
           RLLLLD      +D    +  EA+KA              E+    L L Y+ ++  E+L
Sbjct: 120 RLLLLDPNSITSSDSFGSDEAEALKAY---------SVPQEIQSYELKLTYENFKSEEIL 179

Query: 342 EALLPKDMIIPSAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINN 401
            A+LP+   + S F  VGHIAH+NLR+  LPY KLI +V++DKN P I  VVNK + I++
Sbjct: 180 RAVLPEGQGVTSGFSRVGHIAHMNLREHQLPYRKLIGQVIIDKN-PGITCVVNKTNTIDS 239

Query: 402 DYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDV 461
            YR  Q+EVLAG  ++V  V ENG+ +  D + VYWN RL+TE  R++S     D V DV
Sbjct: 240 TYRNFQMEVLAGESNMVAKVRENGVLYEFDFSRVYWNPRLSTEHERIVSLLHRGDTVVDV 299

Query: 462 FAGVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFI----- 521
           FAGVGP +I AAR    V ANDLNP +  +L+ N+  NK+++KI   N++GR FI     
Sbjct: 300 FAGVGPFAIPAARRGCAVLANDLNPESFRWLQHNAKLNKVDQKITTSNLDGRDFIRGPVR 359

Query: 522 SAIFASEKAPKITQVVMNLPNDAAEYLDAFKGILRDRSDIEFT-LPMIHVYGFSKTRDPE 581
             + A  K  +   VVMNLP  A E+LDAFKG+L    D   + LP +H YGFSK  DP+
Sbjct: 360 ERLPALMKGSQKIHVVMNLPALALEFLDAFKGLLDPEPDQSLSNLPQVHCYGFSKENDPQ 419

Query: 582 FDFHERIRIAL-TEVAVDVDMRRVRLVAPGKWMLCASFRLPRTV 616
            D  ER   +L T +     +  VR VAP K M+C SF LPR V
Sbjct: 420 RDVVERAEASLKTNLQGQCSVHLVRNVAPNKEMMCVSFTLPRGV 453

BLAST of Cla023434 vs. Swiss-Prot
Match: TRM5_PHYIT (tRNA (guanine(37)-N1)-methyltransferase OS=Phytophthora infestans (strain T30-4) GN=PITG_12867 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.0e-63
Identity = 157/405 (38.77%), Postives = 228/405 (56.30%), Query Frame = 1

Query: 222 MSRPKKRKEKKEEGRDE-GTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGS 281
           MS+P   K   ++  D  G R   K     V+ +    L +  ++ ++ D      ++ S
Sbjct: 1   MSKPLLDKTLFKKTLDVVGVRVEAKKIGRVVKKLHGHLLNLPRLRNVVPDPTNPDPYKNS 60

Query: 282 T-RLLLLDERYAD-KGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVL 341
           + +L+LL+ +  D + ++ L E + A LKE++          V   + L Y Y+ +++VL
Sbjct: 61  SSKLILLNSKVKDMETLQPLNEDLVAFLKEES-------LAFVSHAIELDYSYFAVDQVL 120

Query: 342 EALLPKDMIIPSAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINN 401
             LLPK M IPS+FETVGHIAHLNLRD  LP+  +I +V+LDKN  +I+TVVNK D I  
Sbjct: 121 SELLPKGMDIPSSFETVGHIAHLNLRDNQLPFKNVIGQVILDKN-AQIRTVVNKTDNIET 180

Query: 402 DYRTMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDV 461
            +RT  +EVLAG+  +   V E+   F  + A VYWNSRL  E  R+I     +DVVCD+
Sbjct: 181 KFRTFPMEVLAGDDDMEVEVHESKASFRFNYAEVYWNSRLQQEHLRIIRQIKPHDVVCDM 240

Query: 462 FAGVGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFA 521
             G+GP ++  A    KVYANDLNP +  YL+ N   NK+E+ +  +N++GR F++ + +
Sbjct: 241 MCGIGPFAVPVALNGSKVYANDLNPRSYHYLKENIALNKVEKLVTAYNLDGRDFLAKLLS 300

Query: 522 SEKAPKITQVVMNLPNDAAEYLDAFKGILRDRSDIEFTLPMIHVYGFSKTRDPEFDFHER 581
            +K  + TQV+MNLP  A E+LDAF G        E  LP +H Y FS   D + D  +R
Sbjct: 301 EKK--QFTQVLMNLPAIALEFLDAFPG---QFDHWEGELPFVHCYCFSNADDVKQDVKQR 360

Query: 582 IRIALTEVAVDVDMRR-----VRLVAPGKWMLCASFRLPRTVALS 619
              A   +  ++D  R     VR VAP K M+C SF+LP ++A S
Sbjct: 361 ---AEKIMGGELDPERTSFHLVRDVAPKKVMVCISFQLPESIAFS 389

HSP 2 Score: 41.2 bits (95), Expect = 4.9e-02
Identity = 18/47 (38.30%), Postives = 26/47 (55.32%), Query Frame = 1

Query: 103 LLDEDAFTRIFDIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVP 150
           LLD+  F +  D+  +RV ++    +  +L GHLLN PR+RN+   P
Sbjct: 5   LLDKTLFKKTLDVVGVRVEAKKIGRVVKKLHGHLLNLPRLRNVVPDP 51

BLAST of Cla023434 vs. TrEMBL
Match: A0A0A0LFW2_CUCSA (tRNA (guanine(37)-N1)-methyltransferase OS=Cucumis sativus GN=Csa_3G851170 PE=3 SV=1)

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 556/620 (89.68%), Postives = 587/620 (94.68%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLYGPSLC 60
           MVTKLFLRPQSLPLTAF+ KFIFP K+ LPICFTSPFSSTT+HSQSQFS QS  YGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAALRV 120
           KG+HP PSVSRQSP++HS PLLKTL GN ERE+LQ++E+E A+LDEDAFTRIFD+AALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGIDSL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAE++E VGEQNWSNSDDGDG  ID L
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRDEGT 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR K+RKEKKEEGRDE  
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGIEELPE 300
           RRMGKS+ A VEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDE+YA KG+EELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360
           AIKALLK DTQE+MKPT ELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL Y KLIAKV+LDKNKPKIQTVVNK D I+NDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATER RL+S FTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDAAEYL 540
           LNPYAVEYLERNSVANKLERKIEVFNMEGRRFI A+FASEKAPKITQVVMNLPNDAAEYL
Sbjct: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFIRAMFASEKAPKITQVVMNLPNDAAEYL 540

Query: 541 DAFKGILRDRSDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRVRLVAP 600
           DAFKGILRDRSD EFTLP+IHVYGFSK RDPEFDFHERIRIA+TEVAVDV++RRVRLVAP
Sbjct: 541 DAFKGILRDRSDKEFTLPIIHVYGFSKARDPEFDFHERIRIAITEVAVDVNVRRVRLVAP 600

Query: 601 GKWMLCASFRLPRTVALSKP 621
           GKWMLCASFRLPR+VALSKP
Sbjct: 601 GKWMLCASFRLPRSVALSKP 620

BLAST of Cla023434 vs. TrEMBL
Match: M5WQ53_PRUPE (tRNA (guanine(37)-N1)-methyltransferase OS=Prunus persica GN=PRUPE_ppa002915mg PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 2.2e-235
Identity = 433/634 (68.30%), Postives = 513/634 (80.91%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFLP----ICFTSPFSSTTIHSQSQFSDQS-FLY 60
           +VTKLFLRPQSL L+   +  + P    LP    I     F++TT   Q Q SD + F Y
Sbjct: 2   VVTKLFLRPQSLLLSTLPSIILLPKISSLPKKLTILSLCNFTTTT---QPQISDPNLFFY 61

Query: 61  GPSLCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQ--NEESEEALLDEDAFTRIF 120
           GPSL KG +P    S+      ++P          R++LQ  NEE EE L++ ++F+R+F
Sbjct: 62  GPSLHKGTNPTIPQSQLPITGFTKPA--------HRQQLQEENEEEEENLINAESFSRVF 121

Query: 121 DIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGD 180
           DIAALRVP++ CF LE+RLRGHLLNWPRIRNIARV GDEVE EL E +   +    D+  
Sbjct: 122 DIAALRVPAKDCFALESRLRGHLLNWPRIRNIARVAGDEVEEELAELLANPSREADDEN- 181

Query: 181 GIGIDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKE 240
              + SL+RR+YGK+EGDGE LSPVLYREKL KTFDS+GYV FR+LAKMSRP +RK++++
Sbjct: 182 --ALVSLNRRMYGKSEGDGELLSPVLYREKLAKTFDSRGYVKFRNLAKMSRPNQRKKRRD 241

Query: 241 EGRD--EGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGK-WRGSTRLLLLDERY 300
           E  +  EG +R+ K + + +EVV+ EE E +D+KGLLGDEF+G K WRGSTRLLLLDER 
Sbjct: 242 EEDEGGEGKKRLRKGEFSVLEVVDDEEEEGEDLKGLLGDEFEGRKKWRGSTRLLLLDERC 301

Query: 301 ADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPS 360
           A+  +E+LPEAIK L+KE+T + M PT ELVRC+LTL YDYWQMNE+LEALLP+ M IPS
Sbjct: 302 ANGSVEDLPEAIKVLMKENTGKSMCPTLELVRCKLTLLYDYWQMNEILEALLPEGMTIPS 361

Query: 361 AFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAG 420
           AFETVGHIAHLNLRDEHLPY KLIAKVVLDK+KPKIQTVVNKID I+NDYRTMQLEVL+G
Sbjct: 362 AFETVGHIAHLNLRDEHLPYKKLIAKVVLDKHKPKIQTVVNKIDAIDNDYRTMQLEVLSG 421

Query: 421 NHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAA 480
           NHSLVTTVVENGLRF +DLA VYWNSRLATER RL++ FT NDVVCDVF+GVGPI++SAA
Sbjct: 422 NHSLVTTVVENGLRFQVDLAKVYWNSRLATERQRLLNSFTRNDVVCDVFSGVGPIAMSAA 481

Query: 481 RIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVM 540
           RIVK+VYANDLNP+A+EYLERNSV NKLERKI+VFNM+GRRFI+A+FAS+KA  IT VVM
Sbjct: 482 RIVKRVYANDLNPHAIEYLERNSVLNKLERKIKVFNMDGRRFINAMFASDKAKSITHVVM 541

Query: 541 NLPNDAAEYLDAFKGILRDRS-DIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVD 600
           NLPNDAAEYLDAF+GIL DRS D  FTLPMIHVYGFSK +DPEFDFH+RIRIAL+EVAVD
Sbjct: 542 NLPNDAAEYLDAFRGILVDRSMDEVFTLPMIHVYGFSKAQDPEFDFHQRIRIALSEVAVD 601

Query: 601 VDMRRVRLVAPGKWMLCASFRLPRTVALSKPTLN 624
           V+MRRVRLVAPGKWMLCASF LP++VA +KPTLN
Sbjct: 602 VEMRRVRLVAPGKWMLCASFILPKSVAFAKPTLN 621

BLAST of Cla023434 vs. TrEMBL
Match: B9SIH1_RICCO (tRNA (guanine(37)-N1)-methyltransferase OS=Ricinus communis GN=RCOM_1256170 PE=3 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 2.4e-226
Identity = 416/638 (65.20%), Postives = 496/638 (77.74%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAF-----TAKFIFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLY 60
           MVTK FLRP S PLT       T  F+FP      + F S FS+T  H  ++ S ++FLY
Sbjct: 1   MVTKFFLRPNSFPLTIISPARTTQFFLFPKLSISKLPFISLFSATPAHPSTK-SPEAFLY 60

Query: 61  GPSLCKGVHPVPSVSRQSPVN-------HSRPLLKTLIGNPERERLQNEE---SEEALLD 120
           GPSL KG +P     +Q  +         S+     L+   E+++ + +E    +++++D
Sbjct: 61  GPSLLKGNNPFQYAQQQQKLQLQQEQCYQSQLGAPKLLNGDEQDKKEEKEIDFHDDSVID 120

Query: 121 EDAFTRIFDIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQN 180
           E+ F R+FDIAALRVP++ CF LE+RLRGHLLNWPRIRNIARVPGDEV+ E+V  +GE  
Sbjct: 121 EENFIRVFDIAALRVPAKNCFALESRLRGHLLNWPRIRNIARVPGDEVDEEIVSLLGESK 180

Query: 181 WSNSDDGDGIG-IDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR 240
             N    D  G +D+L RRIYG+AEGDGE LSPVLYRE+L K F+S+G+  FR+LAK+SR
Sbjct: 181 VGNGSRDDEEGKLDALDRRIYGRAEGDGEELSPVLYRERLAKEFNSRGFFKFRNLAKISR 240

Query: 241 P--KKRKEKKEEGRDEGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGK-WRGST 300
           P  +KRKE + EG ++  ++ GK + A VEV+E+++ E  D KGLLGD+FKG K WRGST
Sbjct: 241 PPKRKRKEGEVEGGEKMRKKNGKDQFAVVEVMEADD-EEGDWKGLLGDDFKGSKKWRGST 300

Query: 301 RLLLLDERYADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEAL 360
           RLLLLDERYADK +E+LP AIKA L+E  +E    T ELVRC+LTL Y+YWQMNEVLEAL
Sbjct: 301 RLLLLDERYADKRMEDLPRAIKAALQEAMKENSTSTFELVRCKLTLSYNYWQMNEVLEAL 360

Query: 361 LPKDMIIPSAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYR 420
           LP+  IIPSAFETVGHIAHLNLR+EH+PY KLIAKVVLDKNKPKIQTVVNKID I NDYR
Sbjct: 361 LPEGAIIPSAFETVGHIAHLNLRNEHMPYKKLIAKVVLDKNKPKIQTVVNKIDAIQNDYR 420

Query: 421 TMQLEVLAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAG 480
           TMQLEVLAGNHSLVT VVENGLRFH+DLA VYWNSRLATER RL+ GFT NDVVCDVF+G
Sbjct: 421 TMQLEVLAGNHSLVTMVVENGLRFHVDLAKVYWNSRLATERQRLLDGFTRNDVVCDVFSG 480

Query: 481 VGPISISAARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEK 540
           VGPI++SAA IVK+VYANDLNP AV+YLERNSV NKLERK++VFNM+GRRFI A+F+S+K
Sbjct: 481 VGPIALSAATIVKRVYANDLNPCAVDYLERNSVLNKLERKVKVFNMDGRRFIDAMFSSDK 540

Query: 541 APKITQVVMNLPNDAAEYLDAFKGILRDR-SDIEFTLPMIHVYGFSKTRDPEFDFHERIR 600
           A  ITQVVMNLPNDAAEYLDAF+G+ +D     E  LPMIHVYGFSK  DPEF+FHERIR
Sbjct: 541 AQTITQVVMNLPNDAAEYLDAFRGLFKDNPKKREVALPMIHVYGFSKAHDPEFEFHERIR 600

Query: 601 IALTEVAVDVDMRRVRLVAPGKWMLCASFRLPRTVALS 619
           IAL E AV V+MRRVRLVAPGKWMLCASFRLP +VA +
Sbjct: 601 IALAEAAVHVEMRRVRLVAPGKWMLCASFRLPESVAFA 636

BLAST of Cla023434 vs. TrEMBL
Match: A0A068UDY4_COFCA (tRNA (guanine(37)-N1)-methyltransferase OS=Coffea canephora GN=GSCOC_T00021142001 PE=3 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.6e-222
Identity = 410/622 (65.92%), Postives = 485/622 (77.97%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFL-PICFTSPFSSTTIHSQSQFSDQSFLYGPSL 60
           MV KL  RP  LP +  +    FP + F  P+ F+   +STT    +  S  +  YGPSL
Sbjct: 1   MVIKLIFRPNCLPFSVLS----FPPRLFSRPLNFSLLSTSTTTAVAAVSS--TLPYGPSL 60

Query: 61  CKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAALR 120
            KG  P P      P  H  P                +ES+  L DED+FTR+FD+AALR
Sbjct: 61  RKGHSPSPF----PPTLHQCPS-------------SEQESDSNLFDEDSFTRVFDLAALR 120

Query: 121 VPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGIDS 180
           VPS  CF LENRLRGHLLNWPR+RNIARVPGDE++AEL +   + + SN+ + D   + +
Sbjct: 121 VPSELCFGLENRLRGHLLNWPRVRNIARVPGDEIDAELKKLFPDCSSSNNLNTDNDALVT 180

Query: 181 LHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRDEG 240
           L+RRIYGKA+GDGE LSPVLYR+KL +TF+S+G+VNFR+LAK+SRPKK K+K+EE +++G
Sbjct: 181 LNRRIYGKADGDGELLSPVLYRDKLARTFNSRGFVNFRNLAKISRPKKVKKKEEERKEKG 240

Query: 241 TRR-MGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGIEEL 300
            +  +G++++A V VVE EE    D+ GLLGDEFKG  WRGSTRLLLLDE YA+KGI E+
Sbjct: 241 KKGGVGRNEMAVVAVVEEEE---SDVSGLLGDEFKGKSWRGSTRLLLLDETYANKGINEM 300

Query: 301 PEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHI 360
           PEAIKA+LKE   +K   T ELV+C+LTLFY YWQMNEVLEALLP  MIIPSAFETVGHI
Sbjct: 301 PEAIKAVLKEYVGDKGDSTFELVKCKLTLFYSYWQMNEVLEALLPNGMIIPSAFETVGHI 360

Query: 361 AHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTV 420
           AHLNLRDEHLPY K++AKVVLDKNKPKIQTVVNK D+I+NDYRTMQLEVLAGNHSLVTTV
Sbjct: 361 AHLNLRDEHLPYKKIVAKVVLDKNKPKIQTVVNKTDIIHNDYRTMQLEVLAGNHSLVTTV 420

Query: 421 VENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKVYA 480
           VENGLRFH+DLATVYWNSRL+TER RL++ FT +DV+CDVFAGVGPI+++AAR VK VYA
Sbjct: 421 VENGLRFHVDLATVYWNSRLSTERQRLLNYFTRDDVICDVFAGVGPIALAAARKVKHVYA 480

Query: 481 NDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDAAE 540
           NDLNPYAV+YLERNSV NKLE+KIEVFNM+GRRFI  IFAS K   +TQVVMNLPNDA E
Sbjct: 481 NDLNPYAVDYLERNSVLNKLEKKIEVFNMDGRRFIDTIFASHKTRPVTQVVMNLPNDATE 540

Query: 541 YLDAFKGILR-DRSDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRVRL 600
           +LDAF+G+ R    D  +TLP IHVYGFSK +DPEFDFHERIRIAL+EVA DV+M RVR+
Sbjct: 541 FLDAFRGLFRKHHKDRGYTLPRIHVYGFSKAQDPEFDFHERIRIALSEVAFDVEMHRVRI 596

Query: 601 VAPGKWMLCASFRLPRTVALSK 620
           VAPGKWMLCASF LP  VA SK
Sbjct: 601 VAPGKWMLCASFTLPERVAYSK 596

BLAST of Cla023434 vs. TrEMBL
Match: A0A067L388_JATCU (tRNA (guanine(37)-N1)-methyltransferase OS=Jatropha curcas GN=JCGZ_00722 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 3.1e-221
Identity = 408/620 (65.81%), Postives = 481/620 (77.58%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKF--IFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLYGPS 60
           MVTK F R  SLPLT  ++    +FP      I  TS  ++  I      + +S LYGPS
Sbjct: 1   MVTKFFPRANSLPLTIISSTHFSLFPNSSLSIISLTSTAATQAI------TPKSHLYGPS 60

Query: 61  LCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAAL 120
           L KG   +P  + QS                   +L  +  EE ++DE+ F+R+FDIAAL
Sbjct: 61  LLKG--RIPFQNSQS-------------------QLDPKPIEEQVIDEEKFSRVFDIAAL 120

Query: 121 RVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGID 180
           RVP++ CF LE+RLRGHLLNWPRIRNIARVPGDEVE ELV  + +    N D+   +  D
Sbjct: 121 RVPAKNCFALESRLRGHLLNWPRIRNIARVPGDEVEEELVSLLDDSKNGNGDEEGKL--D 180

Query: 181 SLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRDE 240
           +L++ IYG+ E  GE L PVLYREKLVK F+ +G+V F++LAK+SRP KRK KKEEG ++
Sbjct: 181 ALNQMIYGRVERGGEELIPVLYREKLVKEFNYRGFVKFKNLAKISRPPKRKRKKEEGEEK 240

Query: 241 GTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGK-WRGSTRLLLLDERYADKGIEE 300
              +  K   + VEVVE EE   +D KGLLGD+FKG K WRGSTRLLLLDERYADKG+E+
Sbjct: 241 MRGKCRKKGFSVVEVVEEEE---EDWKGLLGDKFKGRKKWRGSTRLLLLDERYADKGVED 300

Query: 301 LPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGH 360
           LP+AI+  L+E  +E    + EL++C+LTLFYDYWQMNEVLEALLP+ +IIPS+FE +GH
Sbjct: 301 LPQAIQVALQEAMEENPTSSFELIKCKLTLFYDYWQMNEVLEALLPEGVIIPSSFEIIGH 360

Query: 361 IAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTT 420
           IAHLNLRDEHLPY  LIAKVVLDKNKPKIQTVVNKID I+NDYRT+ LEVLAGNHSLVT 
Sbjct: 361 IAHLNLRDEHLPYKNLIAKVVLDKNKPKIQTVVNKIDAIHNDYRTLPLEVLAGNHSLVTM 420

Query: 421 VVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKVY 480
           VVE+GLRFH+DLATVYWNSRLA ER RL++GFT NDVVCDVFAGVGPI++SAA+IVK+VY
Sbjct: 421 VVESGLRFHVDLATVYWNSRLANERQRLLNGFTRNDVVCDVFAGVGPIALSAAKIVKRVY 480

Query: 481 ANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDAA 540
           ANDLNPYAV+YLERNSV NKLERKI+VFNM+GRRFI A+ +SEKA  ITQVVMNLPNDAA
Sbjct: 481 ANDLNPYAVDYLERNSVLNKLERKIQVFNMDGRRFIKAMLSSEKAQTITQVVMNLPNDAA 540

Query: 541 EYLDAFKGILRD-RSDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRVR 600
           EYLDAF+GI +D     EFTLPMIHVYGFSK RDPEFDFHERIRIALTEVAVDV+M +VR
Sbjct: 541 EYLDAFRGIFKDNHKRREFTLPMIHVYGFSKARDPEFDFHERIRIALTEVAVDVEMHKVR 588

Query: 601 LVAPGKWMLCASFRLPRTVA 617
           LVAPGKWMLCASFRLP +VA
Sbjct: 601 LVAPGKWMLCASFRLPESVA 588

BLAST of Cla023434 vs. NCBI nr
Match: gi|659109254|ref|XP_008454621.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 566/625 (90.56%), Postives = 591/625 (94.56%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLYGPSLC 60
           MVTKLFL PQSLPLTAFT KFIFP K+FLPICFTSPF STT+HSQSQFS QS  YGPSLC
Sbjct: 1   MVTKLFLTPQSLPLTAFTPKFIFPRKNFLPICFTSPFYSTTVHSQSQFSHQSLPYGPSLC 60

Query: 61  KGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAALRV 120
           KG+HP PSVSRQSP++HS PLLKTL GN ERE+LQNEESE A+LDEDAFTRIFD+AALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHS-PLLKTLTGNLEREQLQNEESEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGIDSL 180
           PS+GCFELENRLRGHLLNWPRI NIARVPGDEVEAELVE VGEQNWS+SDDGDG  ID L
Sbjct: 121 PSKGCFELENRLRGHLLNWPRIHNIARVPGDEVEAELVELVGEQNWSSSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRDEGT 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR K+RKEKKEEGRDE  
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEEM 240

Query: 241 RRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGIEELPE 300
           RRMGKS+ A VEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDE+YA KG+ ELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDEQYAAKGVVELPE 300

Query: 301 AIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360
           AIKALLK DTQE+MKPT ELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHLPY KLIAKVVLDKNKPKIQTVVNK D I+NDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLPYKKLIAKVVLDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATER RL+S FTCNDVVCDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDAAEYL 540
           LNPYAVEYLERNSVANKLERKIEVFNMEGRRFISA+FASEKAPKITQVVMNLPNDAAEYL
Sbjct: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAMFASEKAPKITQVVMNLPNDAAEYL 540

Query: 541 DAFKGILRDRSDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRVRLVAP 600
           DAFKGILRDRSD EFTLP+IHVYGFSK RDPEFDFHERIRIA+TEVAVDV++RRVR VAP
Sbjct: 541 DAFKGILRDRSDKEFTLPIIHVYGFSKARDPEFDFHERIRIAITEVAVDVNVRRVRHVAP 600

Query: 601 GKWMLCASFRLPRTVALSKPTLNNM 626
           GKWMLCASFRLPR+VALSKPTLN++
Sbjct: 601 GKWMLCASFRLPRSVALSKPTLNDI 624

BLAST of Cla023434 vs. NCBI nr
Match: gi|449458211|ref|XP_004146841.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1 [Cucumis sativus])

HSP 1 Score: 1120.1 bits (2896), Expect = 0.0e+00
Identity = 556/620 (89.68%), Postives = 587/620 (94.68%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLYGPSLC 60
           MVTKLFLRPQSLPLTAF+ KFIFP K+ LPICFTSPFSSTT+HSQSQFS QS  YGPSLC
Sbjct: 1   MVTKLFLRPQSLPLTAFSPKFIFPHKNSLPICFTSPFSSTTVHSQSQFSHQSLSYGPSLC 60

Query: 61  KGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAALRV 120
           KG+HP PSVSRQSP++HS PLLKTL GN ERE+LQ++E+E A+LDEDAFTRIFD+AALRV
Sbjct: 61  KGIHPFPSVSRQSPIDHSPPLLKTLTGNLEREQLQDQENEGAILDEDAFTRIFDVAALRV 120

Query: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGIDSL 180
           PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAE++E VGEQNWSNSDDGDG  ID L
Sbjct: 121 PSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAEMLELVGEQNWSNSDDGDGTSIDLL 180

Query: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRDEGT 240
           HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSR K+RKEKKEEGRDE  
Sbjct: 181 HRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRLKRRKEKKEEGRDEKM 240

Query: 241 RRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGKWRGSTRLLLLDERYADKGIEELPE 300
           RRMGKS+ A VEVVESEELE+DDMKGLLGD FKGGKWRGSTRLLLLDE+YA KG+EELPE
Sbjct: 241 RRMGKSEFAIVEVVESEELEMDDMKGLLGDGFKGGKWRGSTRLLLLDEQYAAKGVEELPE 300

Query: 301 AIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVGHIAH 360
           AIKALLK DTQE+MKPT ELVRCRLTLFYDYWQMNEVL+ALLPKDMIIPSAFETVGHIAH
Sbjct: 301 AIKALLKGDTQERMKPTIELVRCRLTLFYDYWQMNEVLQALLPKDMIIPSAFETVGHIAH 360

Query: 361 LNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVTTVVE 420
           LNLRDEHL Y KLIAKV+LDKNKPKIQTVVNK D I+NDYRTMQLEVLAGNHSLVTTVVE
Sbjct: 361 LNLRDEHLSYKKLIAKVILDKNKPKIQTVVNKSDAISNDYRTMQLEVLAGNHSLVTTVVE 420

Query: 421 NGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKVYAND 480
           NGLRFHLDLATVYWNSRLATER RL+S FTCNDV+CDVFAGVGPISISAARIVKKVYAND
Sbjct: 421 NGLRFHLDLATVYWNSRLATERQRLLSVFTCNDVICDVFAGVGPISISAARIVKKVYAND 480

Query: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDAAEYL 540
           LNPYAVEYLERNSVANKLERKIEVFNMEGRRFI A+FASEKAPKITQVVMNLPNDAAEYL
Sbjct: 481 LNPYAVEYLERNSVANKLERKIEVFNMEGRRFIRAMFASEKAPKITQVVMNLPNDAAEYL 540

Query: 541 DAFKGILRDRSDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRVRLVAP 600
           DAFKGILRDRSD EFTLP+IHVYGFSK RDPEFDFHERIRIA+TEVAVDV++RRVRLVAP
Sbjct: 541 DAFKGILRDRSDKEFTLPIIHVYGFSKARDPEFDFHERIRIAITEVAVDVNVRRVRLVAP 600

Query: 601 GKWMLCASFRLPRTVALSKP 621
           GKWMLCASFRLPR+VALSKP
Sbjct: 601 GKWMLCASFRLPRSVALSKP 620

BLAST of Cla023434 vs. NCBI nr
Match: gi|470119549|ref|XP_004295875.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1 isoform X1 [Fragaria vesca subsp. vesca])

HSP 1 Score: 830.1 bits (2143), Expect = 2.6e-237
Identity = 428/625 (68.48%), Postives = 499/625 (79.84%), Query Frame = 1

Query: 1   MVTKLFL-RPQSLPLTAFTAKFIFPGKHFLPICFTSPFSSTTIHSQSQFSDQSFLYGPSL 60
           MV  LFL RP SLPL    +KF+FP    LP  F  P  S +  +  Q  D    YGPSL
Sbjct: 1   MVANLFLIRPHSLPLFTLPSKFLFPNPPSLPKRF--PILSLSFSTHPQIPDPDLSYGPSL 60

Query: 61  CKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRIFDIAALR 120
            KG  P   +    P++  +P+                  EEAL+DE++FTR+F+IAALR
Sbjct: 61  HKGTKPPLPI----PLSTDQPI------------------EEALIDEESFTRVFNIAALR 120

Query: 121 VPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGDGIGIDS 180
           VP++ CF LE+RLRGHLLNWPR+RNI RVPGDEVE E+ E +   N  N ++ D  G+ S
Sbjct: 121 VPAKDCFALESRLRGHLLNWPRVRNIGRVPGDEVEDEIAELLA--NNQNGEEEDQNGVVS 180

Query: 181 LHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKEEGRD-- 240
           L+RR+YGK+EGDGEPLSPVLYREKL KTFDS+GYVNFR+LAKMSRP ++K +  EG D  
Sbjct: 181 LNRRVYGKSEGDGEPLSPVLYREKLAKTFDSRGYVNFRNLAKMSRPNQKKRRAAEGGDGG 240

Query: 241 EGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGK-WRGSTRLLLLDERYADKGIE 300
           EG RR  KS+ + +EVVE E+ E +D+KGLLGD F+G K WRGSTRLLLLDERY DKG+E
Sbjct: 241 EGRRRNRKSEFSVLEVVEDEDEEGEDLKGLLGDAFQGRKKWRGSTRLLLLDERYGDKGVE 300

Query: 301 ELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPSAFETVG 360
           ELP AI+A++KEDT + M+PT ELVRC+LTLFYDYWQMNE+LEALLP+ MI+PSAFETVG
Sbjct: 301 ELPRAIQAVVKEDTGKSMRPTIELVRCKLTLFYDYWQMNEILEALLPEGMIVPSAFETVG 360

Query: 361 HIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAGNHSLVT 420
           HIAHLNLRDEHLPY K IAKV+LDKNKPKIQTVVNKIDVI NDYRTMQLEVLAGNHSLVT
Sbjct: 361 HIAHLNLRDEHLPYRKFIAKVILDKNKPKIQTVVNKIDVIQNDYRTMQLEVLAGNHSLVT 420

Query: 421 TVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAARIVKKV 480
            VVENGLRF +DLATVYWNSRLATER RL++ FT NDVV DVF+GVGPI+ISAA+IVK+V
Sbjct: 421 KVVENGLRFQVDLATVYWNSRLATERQRLLNSFTRNDVVSDVFSGVGPIAISAAKIVKRV 480

Query: 481 YANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVMNLPNDA 540
           YANDLNP+AVEYL+RN   NKL++K++VFNM+GRRFI A+FAS+KA  ITQVVMNLP DA
Sbjct: 481 YANDLNPHAVEYLQRNCGLNKLDKKVKVFNMDGRRFIRAMFASDKAKSITQVVMNLPKDA 540

Query: 541 AEYLDAFKGILRDRS-DIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVDVDMRRV 600
           AEYLDAF+GILRDRS D EF LPMIHVYGFSK  DP+FDFH+RIRIAL EVAVDV+MRRV
Sbjct: 541 AEYLDAFRGILRDRSKDEEFNLPMIHVYGFSKAEDPDFDFHQRIRIALAEVAVDVEMRRV 599

Query: 601 RLVAPGKWMLCASFRLPRTVALSKP 621
           RLVAPGKWMLCASF LP+ VA + P
Sbjct: 601 RLVAPGKWMLCASFILPKNVAFANP 599

BLAST of Cla023434 vs. NCBI nr
Match: gi|595913904|ref|XP_007214625.1| (hypothetical protein PRUPE_ppa002915mg [Prunus persica])

HSP 1 Score: 823.2 bits (2125), Expect = 3.2e-235
Identity = 433/634 (68.30%), Postives = 513/634 (80.91%), Query Frame = 1

Query: 1   MVTKLFLRPQSLPLTAFTAKFIFPGKHFLP----ICFTSPFSSTTIHSQSQFSDQS-FLY 60
           +VTKLFLRPQSL L+   +  + P    LP    I     F++TT   Q Q SD + F Y
Sbjct: 2   VVTKLFLRPQSLLLSTLPSIILLPKISSLPKKLTILSLCNFTTTT---QPQISDPNLFFY 61

Query: 61  GPSLCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQ--NEESEEALLDEDAFTRIF 120
           GPSL KG +P    S+      ++P          R++LQ  NEE EE L++ ++F+R+F
Sbjct: 62  GPSLHKGTNPTIPQSQLPITGFTKPA--------HRQQLQEENEEEEENLINAESFSRVF 121

Query: 121 DIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDGD 180
           DIAALRVP++ CF LE+RLRGHLLNWPRIRNIARV GDEVE EL E +   +    D+  
Sbjct: 122 DIAALRVPAKDCFALESRLRGHLLNWPRIRNIARVAGDEVEEELAELLANPSREADDEN- 181

Query: 181 GIGIDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKKE 240
              + SL+RR+YGK+EGDGE LSPVLYREKL KTFDS+GYV FR+LAKMSRP +RK++++
Sbjct: 182 --ALVSLNRRMYGKSEGDGELLSPVLYREKLAKTFDSRGYVKFRNLAKMSRPNQRKKRRD 241

Query: 241 EGRD--EGTRRMGKSKVAAVEVVESEELEVDDMKGLLGDEFKGGK-WRGSTRLLLLDERY 300
           E  +  EG +R+ K + + +EVV+ EE E +D+KGLLGDEF+G K WRGSTRLLLLDER 
Sbjct: 242 EEDEGGEGKKRLRKGEFSVLEVVDDEEEEGEDLKGLLGDEFEGRKKWRGSTRLLLLDERC 301

Query: 301 ADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMIIPS 360
           A+  +E+LPEAIK L+KE+T + M PT ELVRC+LTL YDYWQMNE+LEALLP+ M IPS
Sbjct: 302 ANGSVEDLPEAIKVLMKENTGKSMCPTLELVRCKLTLLYDYWQMNEILEALLPEGMTIPS 361

Query: 361 AFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEVLAG 420
           AFETVGHIAHLNLRDEHLPY KLIAKVVLDK+KPKIQTVVNKID I+NDYRTMQLEVL+G
Sbjct: 362 AFETVGHIAHLNLRDEHLPYKKLIAKVVLDKHKPKIQTVVNKIDAIDNDYRTMQLEVLSG 421

Query: 421 NHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISISAA 480
           NHSLVTTVVENGLRF +DLA VYWNSRLATER RL++ FT NDVVCDVF+GVGPI++SAA
Sbjct: 422 NHSLVTTVVENGLRFQVDLAKVYWNSRLATERQRLLNSFTRNDVVCDVFSGVGPIAMSAA 481

Query: 481 RIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQVVM 540
           RIVK+VYANDLNP+A+EYLERNSV NKLERKI+VFNM+GRRFI+A+FAS+KA  IT VVM
Sbjct: 482 RIVKRVYANDLNPHAIEYLERNSVLNKLERKIKVFNMDGRRFINAMFASDKAKSITHVVM 541

Query: 541 NLPNDAAEYLDAFKGILRDRS-DIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEVAVD 600
           NLPNDAAEYLDAF+GIL DRS D  FTLPMIHVYGFSK +DPEFDFH+RIRIAL+EVAVD
Sbjct: 542 NLPNDAAEYLDAFRGILVDRSMDEVFTLPMIHVYGFSKAQDPEFDFHQRIRIALSEVAVD 601

Query: 601 VDMRRVRLVAPGKWMLCASFRLPRTVALSKPTLN 624
           V+MRRVRLVAPGKWMLCASF LP++VA +KPTLN
Sbjct: 602 VEMRRVRLVAPGKWMLCASFILPKSVAFAKPTLN 621

BLAST of Cla023434 vs. NCBI nr
Match: gi|694404890|ref|XP_009377307.1| (PREDICTED: tRNA (guanine(37)-N1)-methyltransferase 1-like [Pyrus x bretschneideri])

HSP 1 Score: 822.4 bits (2123), Expect = 5.4e-235
Identity = 431/637 (67.66%), Postives = 509/637 (79.91%), Query Frame = 1

Query: 1   MVTKLFLRP-QSLPLTAFTAKF-IFPG-----KHFLPICFTSPFSSTTIHSQ-SQFSDQS 60
           MV KL LRP  SLPL+   +KF IFP      K FL +   S  ++   H+Q S  ++ S
Sbjct: 1   MVAKLVLRPPHSLPLSTLPSKFLIFPKIPSLPKRFLIVSIFSATAAAQTHTQISNPNNSS 60

Query: 61  FLYGPSLCKGVHPVPSVSRQSPVNHSRPLLKTLIGNPERERLQNEESEEALLDEDAFTRI 120
             YGPSL KG HP+                 TL  +P   + +++  +  L +E++F+R+
Sbjct: 61  NFYGPSLRKGTHPL-----------------TLRSHPPFTKTEDDYDDGDLFNEESFSRV 120

Query: 121 FDIAALRVPSRGCFELENRLRGHLLNWPRIRNIARVPGDEVEAELVEFVGEQNWSNSDDG 180
           FDIAALRVP++ CF LE+RLRGHLLNWPRIRNI RVPGDEVEAEL E +  Q     D+ 
Sbjct: 121 FDIAALRVPAKDCFALESRLRGHLLNWPRIRNIGRVPGDEVEAELAELLANQGRDLEDEN 180

Query: 181 DGIGIDSLHRRIYGKAEGDGEPLSPVLYREKLVKTFDSKGYVNFRHLAKMSRPKKRKEKK 240
               + SL+RR+YGKAEGDGEP SPVLYREKL KTFDS+GYV FR+LA+MSRP +RK++ 
Sbjct: 181 ---ALASLNRRMYGKAEGDGEPFSPVLYREKLAKTFDSRGYVKFRNLARMSRPNQRKKRG 240

Query: 241 EEGRD--EGTRRMGKSKVAAVEVVESEELEVD--DMKGLLGDEFKGG-KWRGSTRLLLLD 300
           +E  +  EG +R GKS+ + +EVV+ EE E +  D+KGLLGDEF+G  KWRGSTRLLLLD
Sbjct: 241 DEEGEGGEGKKRWGKSEFSVLEVVDDEEGEGEGEDLKGLLGDEFEGRRKWRGSTRLLLLD 300

Query: 301 ERYADKGIEELPEAIKALLKEDTQEKMKPTTELVRCRLTLFYDYWQMNEVLEALLPKDMI 360
           ERYA KG+EELPEAIK  +KE+    M+P  ELVRC+LT+FY+YWQMNE+LEALLP+ MI
Sbjct: 301 ERYAKKGVEELPEAIKVAMKENIGTSMRPAFELVRCKLTVFYEYWQMNEILEALLPEGMI 360

Query: 361 IPSAFETVGHIAHLNLRDEHLPYNKLIAKVVLDKNKPKIQTVVNKIDVINNDYRTMQLEV 420
           +PSAFETVGHIAHLNLRDEHLPY KLIAKV+LDKNKPKIQTVVNKID I+NDYRTMQLEV
Sbjct: 361 VPSAFETVGHIAHLNLRDEHLPYKKLIAKVILDKNKPKIQTVVNKIDAIHNDYRTMQLEV 420

Query: 421 LAGNHSLVTTVVENGLRFHLDLATVYWNSRLATERHRLISGFTCNDVVCDVFAGVGPISI 480
           LAGN SLVTTVVENGLRFH+DLATVYWNSRLATER RL++ FT  DVVCDVF GVGPI++
Sbjct: 421 LAGNRSLVTTVVENGLRFHVDLATVYWNSRLATERQRLVNSFTRKDVVCDVFTGVGPIAM 480

Query: 481 SAARIVKKVYANDLNPYAVEYLERNSVANKLERKIEVFNMEGRRFISAIFASEKAPKITQ 540
           SAARIVK+VYANDLNP A+EYLERNSV NKLERKI+VFNM+GRRFI A+FAS+KA  ITQ
Sbjct: 481 SAARIVKRVYANDLNPNAIEYLERNSVINKLERKIKVFNMDGRRFIGAMFASDKAKSITQ 540

Query: 541 VVMNLPNDAAEYLDAFKGILRDR-SDIEFTLPMIHVYGFSKTRDPEFDFHERIRIALTEV 600
           VVMNLPNDAAEYLDAF+GIL DR ++ EFTLPMIHVYGFSK +DPEFDFH+RIRIAL+E 
Sbjct: 541 VVMNLPNDAAEYLDAFRGILVDRPTEEEFTLPMIHVYGFSKAQDPEFDFHQRIRIALSEA 600

Query: 601 AVDVDMRRVRLVAPGKWMLCASFRLPRTVALSKPTLN 624
           AVDV+MRRVRLVAPGKWMLCASF LP++VA +KPTL+
Sbjct: 601 AVDVEMRRVRLVAPGKWMLCASFILPKSVAYAKPTLD 617