Cla022566 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla022566
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	EF hand family protein (AHRD V1 ***- B4G1S0_MAIZE); contains Interpro domain(s) IPR011992 EF-Hand type
Location	Chr8 : 24650522 .. 24650785 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

mRNA sequence

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

Coding sequence (CDS)

ATGTCTCGTGCATCAAGCAGGCTACAACTCTCCAGAGAGGATATAAAGGAGATTTTCAGAGAGCACGACATCGACGGAGATGGTCGATTGAGCATGAACGAGGTGATGAAGGCCTTCGGGTTCTTAGGTAGTATTCTTCCTTTCTACAAGGCTCACTATGGAATGGCTCATGCTGATGCTGATAACGATGGCTACATCGTCGAGGATGAGCTCGACAAGCTCGTTGACTATGCTGAGAAATTTCAACACAAAAGACAATGTTGA

Protein sequence

MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGYIVEDELDKLVDYAEKFQHKRQC

Homology

[Close]

BLAST of Cla022566 vs. TrEMBL
Match: A0A0A0LRC1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.6e-35
Identity = 73/87 (83.91%), Postives = 82/87 (94.25%), Query Frame = 1


Query: 1  MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
          MSRA++RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DNDGYIVEDELDKLVDYAEKFQHKRQC 88
          + DG+I E+ELDKLVDYA+KFQHKR+C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of Cla022566 vs. TrEMBL
Match: A0A0A0LKX1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1)

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  SRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGY 65
          S+   SRE++KEIFREHDIDGDG LS++E++KA GFLG  +PFYKAHYGMA+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IVEDELDKLVDYAEKFQHKRQ 87
          I EDELDKL+DYAE+FQ +R+
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of Cla022566 vs. TrEMBL
Match: A0A0A0KIY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 1.9e-20
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ HD D DG L++ EV KAF FLG+ILP YKA++GM +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I +DEL+KLVDYA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of Cla022566 vs. TrEMBL
Match: A0A0A0KMQ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 1.4e-18
Identity = 50/85 (58.82%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
           + R SS + LSRE IKEIF+ HD + DG L+  E+ KAF FLGS+ PFYKA YGM +ADA
Sbjct: 87  LDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADA 146

Query: 61  DNDGYIVEDELDKLVDYAEKFQHKR 86
           + DG I E ELDKL+DYA K   K+
Sbjct: 147 NEDGLISEAELDKLIDYASKIIKKK 171

[Close]

BLAST of Cla022566 vs. TrEMBL
Match: A0A0A0KLF3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 6.8e-18
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ +D D DG L++ EV KAF  LGSI PF KA++GM +AD + DG
Sbjct: 120 ASNMQFSREQIKEIFQYYDSDRDGFLNIREVTKAFALLGSIFPFNKAYHGMVYADTNKDG 179

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I EDELDKL++YA KF  K+
Sbjct: 180 LIGEDELDKLINYANKFMKKK 200

[Close]

BLAST of Cla022566 vs. NCBI nr
Match: gi|700207397|gb|KGN62516.1| (hypothetical protein Csa_2G358870 [Cucumis sativus])

HSP 1 Score: 156.4 bits (394), Expect = 2.3e-35
Identity = 73/87 (83.91%), Postives = 82/87 (94.25%), Query Frame = 1


Query: 1  MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
          MSRA++RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD 
Sbjct: 1  MSRAATRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADE 60

Query: 61 DNDGYIVEDELDKLVDYAEKFQHKRQC 88
          + DG+I E+ELDKLVDYA+KFQHKR+C
Sbjct: 61 NGDGFIEEEELDKLVDYAQKFQHKRKC 87

[Close]

BLAST of Cla022566 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-33
Identity = 70/83 (84.34%), Postives = 78/83 (93.98%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           S+RLQLSREDIKEIFREHDIDGDGRLSMNE++KAFG  GSILPFYKAHYGMAHAD + DG
Sbjct: 86  STRLQLSREDIKEIFREHDIDGDGRLSMNEMVKAFGCFGSILPFYKAHYGMAHADENGDG 145

Query: 65  YIVEDELDKLVDYAEKFQHKRQC 88
           +I E+ELDKLVDYA+KFQHKR+C
Sbjct: 146 FIEEEELDKLVDYAQKFQHKRKC 168

[Close]

BLAST of Cla022566 vs. NCBI nr
Match: gi|449470198|ref|XP_004152805.1| (PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus])

HSP 1 Score: 90.1 bits (222), Expect = 2.0e-15
Identity = 41/78 (52.56%), Postives = 57/78 (73.08%), Query Frame = 1


Query: 3  RASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADN 62
          +  S  +L +E++KE+FREHD +GDGRLS+ E+ +AFG  G+  P Y+A +G+  AD D 
Sbjct: 8  KKKSGYKLQKEEMKEMFREHDFNGDGRLSIKELSRAFGQFGAFFPLYRAAFGLFLADDDG 67

Query: 63 DGYIVEDELDKLVDYAEK 81
          DG+I E ELDK+VDYA K
Sbjct: 68 DGFISEQELDKVVDYAIK 85

HSP 2 Score: 120.2 bits (300), Expect = 1.8e-24
Identity = 54/81 (66.67%), Postives = 68/81 (83.95%), Query Frame = 1


Query: 6  SRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDGY 65
          S+   SRE++KEIFREHDIDGDG LS++E++KA GFLG  +PFYKAHYGMA+ D + DG 
Sbjct: 7  SKFNFSREEMKEIFREHDIDGDGYLSISELIKAMGFLGHSIPFYKAHYGMAYCDENGDGL 66

Query: 66 IVEDELDKLVDYAEKFQHKRQ 87
          I EDELDKL+DYAE+FQ +R+
Sbjct: 67 ISEDELDKLIDYAERFQKRRR 87

[Close]

BLAST of Cla022566 vs. NCBI nr
Match: gi|700194513|gb|KGN49690.1| (hypothetical protein Csa_5G068170 [Cucumis sativus])

HSP 1 Score: 106.3 bits (264), Expect = 2.7e-20
Identity = 51/81 (62.96%), Postives = 64/81 (79.01%), Query Frame = 1


Query: 5   SSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADADNDG 64
           +S +Q SRE IKEIF+ HD D DG L++ EV KAF FLG+ILP YKA++GM +ADA+ DG
Sbjct: 96  ASNMQFSREQIKEIFQYHDSDNDGFLNLREVTKAFAFLGTILPLYKAYHGMVYADANKDG 155

Query: 65  YIVEDELDKLVDYAEKFQHKR 86
            I +DEL+KLVDYA KF +KR
Sbjct: 156 LISKDELEKLVDYAYKFFNKR 176

[Close]

BLAST of Cla022566 vs. NCBI nr
Match: gi|700194510|gb|KGN49687.1| (hypothetical protein Csa_5G067410 [Cucumis sativus])

HSP 1 Score: 100.1 bits (248), Expect = 2.0e-18
Identity = 50/85 (58.82%), Postives = 61/85 (71.76%), Query Frame = 1


Query: 1   MSRASSRLQLSREDIKEIFREHDIDGDGRLSMNEVMKAFGFLGSILPFYKAHYGMAHADA 60
           + R SS + LSRE IKEIF+ HD + DG L+  E+ KAF FLGS+ PFYKA YGM +ADA
Sbjct: 87  LDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADA 146

Query: 61  DNDGYIVEDELDKLVDYAEKFQHKR 86
           + DG I E ELDKL+DYA K   K+
Sbjct: 147 NEDGLISEAELDKLIDYASKIIKKK 171

The following BLAST results are available for this feature:

BLAST of Cla022566 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LRC1_CUCSA	1.6e-35	83.91	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G358870 PE=4 SV=1	[more]
A0A0A0LKX1_CUCSA	1.3e-24	66.67	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G359940 PE=4 SV=1	[more]
A0A0A0KIY4_CUCSA	1.9e-20	62.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G068170 PE=4 SV=1	[more]
A0A0A0KMQ1_CUCSA	1.4e-18	58.82	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067410 PE=4 SV=1	[more]
A0A0A0KLF3_CUCSA	6.8e-18	58.02	Uncharacterized protein OS=Cucumis sativus GN=Csa_5G067670 PE=4 SV=1	[more]

BLAST of Cla022566 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700207397\|gb\|KGN62516.1\|	2.3e-35	83.91	hypothetical protein Csa_2G358870 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	1.3e-33	84.34	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|449470198\|ref\|XP_004152805.1\|	2.0e-15	52.56	PREDICTED: probable calcium-binding protein CML10 [Cucumis sativus]	[more]
gi\|700194513\|gb\|KGN49690.1\|	2.7e-20	62.96	hypothetical protein Csa_5G068170 [Cucumis sativus]	[more]
gi\|700194510\|gb\|KGN49687.1\|	2.0e-18	58.82	hypothetical protein Csa_5G067410 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR002048	EF_hand_dom
IPR011992	EF-hand-dom_pair
IPR018247	EF_Hand_1_Ca_BS

Vocabulary: **Molecular Function**
Term	Definition
GO:0005509	calcium ion binding

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0046872	metal ion binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU78860	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla022566	Cla022566.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU78860	WMU78860	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 2..38 score: 2.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 10..45 score: 14.179coord: 55..81 score:
IPR011992	EF-hand domain pair	GENE3D	G3DSA:1.10.238.10		coord: 11..77 score: 3.8
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 6..73 score: 6.07
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 23..35 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..78 score: 6.4
None	No IPR available	PANTHER	PTHR10891:SF592	CALCIUM-BINDING PROTEIN CML38-RELATED	coord: 5..78 score: 6.4

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla022566	Cucsa.232850	Cucumber (Gy14) v1	cgywmB433
Cla022566	MELO3C010866.2	Melon (DHL92) v3.6.1	medwmB295

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla022566	Silver-seed gourd	carwmB0075
Cla022566	Silver-seed gourd	carwmB0172
Cla022566	Silver-seed gourd	carwmB0766
Cla022566	Cucumber (Chinese Long) v3	cucwmB124
Cla022566	Cucumber (Chinese Long) v3	cucwmB310
Cla022566	Watermelon (97103) v2	wmwmbB100
Cla022566	Watermelon (97103) v2	wmwmbB091
Cla022566	Wax gourd	wgowmB297
Cla022566	Wax gourd	wgowmB601
Cla022566	Watermelon (97103) v1	wmwmB059
Cla022566	Watermelon (97103) v1	wmwmB072
Cla022566	Cucumber (Gy14) v1	cgywmB195
Cla022566	Cucumber (Gy14) v1	cgywmB422
Cla022566	Cucurbita maxima (Rimu)	cmawmB014
Cla022566	Cucurbita maxima (Rimu)	cmawmB148
Cla022566	Cucurbita maxima (Rimu)	cmawmB422
Cla022566	Cucurbita maxima (Rimu)	cmawmB484
Cla022566	Cucurbita maxima (Rimu)	cmawmB753
Cla022566	Cucurbita moschata (Rifu)	cmowmB007
Cla022566	Cucurbita moschata (Rifu)	cmowmB081
Cla022566	Cucurbita moschata (Rifu)	cmowmB134
Cla022566	Cucurbita moschata (Rifu)	cmowmB416
Cla022566	Cucurbita moschata (Rifu)	cmowmB748
Cla022566	Wild cucumber (PI 183967)	cpiwmB116
Cla022566	Wild cucumber (PI 183967)	cpiwmB300
Cla022566	Wild cucumber (PI 183967)	cpiwmB597
Cla022566	Melon (DHL92) v3.5.1	mewmB218
Cla022566	Melon (DHL92) v3.5.1	mewmB300
Cla022566	Melon (DHL92) v3.5.1	mewmB501
Cla022566	Watermelon (Charleston Gray)	wcgwmB273
Cla022566	Watermelon (Charleston Gray)	wcgwmB400
Cla022566	Cucumber (Chinese Long) v2	cuwmB111
Cla022566	Cucumber (Chinese Long) v2	cuwmB298
Cla022566	Cucumber (Chinese Long) v2	cuwmB576
Cla022566	Cucurbita pepo (Zucchini)	cpewmB101
Cla022566	Cucurbita pepo (Zucchini)	cpewmB541
Cla022566	Cucurbita pepo (Zucchini)	cpewmB756
Cla022566	Cucurbita pepo (Zucchini)	cpewmB782
Cla022566	Bottle gourd (USVL1VR-Ls)	lsiwmB293
Cla022566	Bottle gourd (USVL1VR-Ls)	lsiwmB465
Cla022566	Cucumber (Gy14) v2	cgybwmB108
Cla022566	Cucumber (Gy14) v2	cgybwmB277
Cla022566	Cucumber (Gy14) v2	cgybwmB539
Cla022566	Melon (DHL92) v3.6.1	medwmB215
Cla022566	Melon (DHL92) v3.6.1	medwmB487