Cla021971 (gene) Watermelon (97103) v1

NameCla021971
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionAsparagine synthetase (AHRD V1 **** F6KDH5_MORAL); contains Interpro domain(s) IPR001962 Asparagine synthase
LocationChr8 : 19191673 .. 19195257 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTCTCAAGCTAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTCAATCGTCTCCTTTCAATTCATTCTTCATACATTCTTTCTTGCTTGTTTCATTTCTTAATCCATATCTCATATATGCCTGATTTGAGTTGAATAATGGAACTTTTAACTTTGTAGAATGTAGAAAACTACAGATGAAATATAATTGACTTGTTGAATAATGATTTCTTTTCTAACATTGAGGCTCTTTTTGATACATTTGTATTGTACCTGTATATATTGTTCGATACGTCGCTTGGGTTTTAGTCGTTGCGTGTTTGTCTATTAAACAGAAAGAAGAAATGAAGTTCGAAGAATTATGGTACTTGTATAATTCTCAATATGAAGTTCAAACAGTAATTTAAATTAATGAACGTTAAACCAGTTAGCGGTAAGGAATTCTGATGATATTTCGTTGTAATATGAAGTATGTTTTGGCCATACTTGGCGCTTGTTGCCATGCAGTGATTTTCGAAAAATAAAATCCATGCCTCCGATTGTGACTTTTCAACTTTTTTATTTTTAACTTTTGGTTGAGAATCAAATAGGATGATCCCCAAGAACGTCGTCGGACAATGTGAAGATAGAAAAGAAAAATAAGACAAAAAAATCATTAATTAGCATGCTTAATCGAATAAATTGTCATATAATCGATATTAACTACTTCTTTTTGTTGGTTTGACAGATTGAAACATCGTGGTCCAGACTGGAGTGGGCTTTACCAGCATGCCGATTTTTATTTAGCCCATCAACGTTTAGCCATTATCGATCCTGCATCTGGTGATCAACCTCTCTATAATGAAGATAAGTCCATTGTTGTCACGGTACACTCTGGAACCTTCTTCGTTGACCTCTTGGTCCCTTCCTACCCGCCCCCAAATGTAATTTATTGTTATGGGTGTGCTTTGCTTCTTCAAATGAGCGAGGAGAAGTAGTCACACAAACACAAACTCTATCTTATGGTGCTCATGTGCAATTGTTTTTTTGCCTAAAAATAGGTCAACGGAGAAATATACAACCACGAGGAGCTGAGGAAGAAATTGAGGAGCCACAAAATTCGAAGCGGCAGTGACTGTGATGTTATTTCACATTTGGTATTAATAGAAATAAATGATTTTGGAAATAATTCTTCCTCCAATAATATAATTTTCCACCAGTTACCCGTTTGGTGCGGTTTAAGGGGATACGATTATGATATTTATCGTTGGTGTCTATGCAGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTTGACGGAATGTTCTCGTTCGTGCTATTGGACACGCGCGACAACAGTTTCATTGTTGCTCGTGATGCAATTGGAATTACTTCCCTTTACATTGGTTGGGGACTCGATGGTAATTAGAATAATTCTAAATCTCATTTGCTCGTCCATTTTAAGCCCTGTAGATGTTCTCTAAGTACTCTTATTTTGATTATATGTTAGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACATTTCCACCGGGTCACTTGTACTCTAGCAAGGACGCTAGATTCAAAAGATGGTATAATCCAACCTGGTTCTCCGAGGATATTCCATCGACCCCATATGACCCACTTGTTCTGAGACGCGCATTGAAGAATGTATAAATTAGCATGATGGATTTGAAGACATGTTATTAAGTTTAACGTATTTTCACATTTCTATTACGCTATCGAGCTCACATGCTAACTTACGCAGGCTGTGATCAAGAGGCTGATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGTCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTTACTTAGCTGGCACAAGAGCTGCCAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGTCTTGAGGTTGGTTTTTGTTTTCTTAGTCTTGGCATTCCTACACAACTATTAATTGTGTACATACTGGGAACCGCTTCTCATTGGGAATAAGATTCGTCTTATAATGTTTAGGGATCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTAGGAACTGTTCACCACGAGTTCCATTTCACAGTTCAGGTACGTCTTATCTAGAACTTTACAAACAAGTATACATCCCTTAATTTCCTTAACCTAATTTTACCATCTTTGTCTTCTTTGATAATAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACACCCATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCTGATGAAATTTTTGGCGGATACCTGTATTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACTGTGAAAGTTGTCGCAAGGTATATGATTTTATGGAGGGCACTGCTCGATTTTTGTATCATTAGGGATGAGAGAAATTGCACTCATATCATAAACCTTTGTTTCTCGCTTGCAGATAAAGGCACTTCACATGTATGATTGCTTAAGAGCAAACAAGTCAGCTTCAGCGTGGGGATTGGAAGTTCGAGTTCCATTTTTAGATAAGGAATTTATTAATGTTGCAATGGCCATTGATCCAGAATGGAAAATGGTAGAGAAATAAACTTTACAATAGCCATAAAGATTAGCCTTTTTCCCTAAATTTTCTTAGCTCAACTTTTTGTTTCTCATCTAATATTTTAATCTATTTTAGATCAAACGTGATCAAGGCCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTTGATGATGAACACCAACCTTATCTCCCTAAGGTCTTTGAAAGAAAAACCTCTCTAGTAGTATACAAAAACTATAACTTCGATAAGAATGTAACATTTTTTCCTGTTTGTAGCATGTTTTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATTGATGGTCTTAAGGCCCATGCTGCTCAACACGTAATTCGATAACCCGTTCCTTTCTATTGGACCTTCGAGCCATTTGGTGTTCTTGTTGAAGAATGAAGTTTAGAAATATAATTTTTTAGAAGCAATAATGTTCATATGCTTGATGATTTATTTACTCAATACATGCTTTCAGGTGACTGATAAAATGATGTTGAACGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTATTATAGAACCATATTTGAGAGGTTTTTCCCACAGGTAATTTAATTCCATCAACAACAGTGTTGACACATGTCTCAGTAAACTTCATTAACCTTATTCCGCACATTGTATGGATGTGCAGAACTCAGCTCGTCTGACTGTCCCGGGAGGACCAAGTATCGCTTGTAGTACGGCAAAGGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGTGTTCATGCCTCAGCATATGGAGAGGAGACAAATAGTGTAAGCGACGTTGGCCCGCCCAAGATAATCGATACCATTTCGAGAATGGAGGTCAATACACCAGGAGTAAAAATCCTTAGCTAG

mRNA sequence

ATGATTCTCAAGCTAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTCAATCATTGAAACATCGTGGTCCAGACTGGAGTGGGCTTTACCAGCATGCCGATTTTTATTTAGCCCATCAACGTTTAGCCATTATCGATCCTGCATCTGGTGATCAACCTCTCTATAATGAAGATAAGTCCATTGTTGTCACGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTTGACGGAATGTTCTCGTTCGTGCTATTGGACACGCGCGACAACAGTTTCATTGTTGCTCGTGATGCAATTGGAATTACTTCCCTTTACATTGGTTGGGGACTCGATGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACATTTCCACCGGGTCACTTGTACTCTAGCAAGGACGCTAGATTCAAAAGATGGTATAATCCAACCTGGTTCTCCGAGGATATTCCATCGACCCCATATGACCCACTTGTTCTGAGACGCGCATTGAAGAATGCTGTGATCAAGAGGCTGATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGTCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTTACTTAGCTGGCACAAGAGCTGCCAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGTCTTGAGGGATCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTAGGAACTGTTCACCACGAGTTCCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACACCCATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCTGATGAAATTTTTGGCGGATACCTGTATTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACTGTGAAAGTTGTCGCAAGATCAAACGTGATCAAGGCCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTTGATGATGAACACCAACCTTATCTCCCTAAGCATGTTTTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATTGATGGTCTTAAGGCCCATGCTGCTCAACACGTGACTGATAAAATGATGTTGAACGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTATTATAGAACCATATTTGAGAGGTTTTTCCCACAGAACTCAGCTCGTCTGACTGTCCCGGGAGGACCAAGTATCGCTTGTAGTACGGCAAAGGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGTGTTCATGCCTCAGCATATGGAGAGGAGACAAATAGTGTAAGCGACGTTGGCCCGCCCAAGATAATCGATACCATTTCGAGAATGGAGGTCAATACACCAGGAGTAAAAATCCTTAGCTAG

Coding sequence (CDS)

ATGATTCTCAAGCTAAACGTGTTCGTGTTCTTGAACTCTCTCGTCGGCAAGTGTTCTCAATCATTGAAACATCGTGGTCCAGACTGGAGTGGGCTTTACCAGCATGCCGATTTTTATTTAGCCCATCAACGTTTAGCCATTATCGATCCTGCATCTGGTGATCAACCTCTCTATAATGAAGATAAGTCCATTGTTGTCACGTATGAAGAATATGGAGAGAATTTTGTGGACATGCTTGACGGAATGTTCTCGTTCGTGCTATTGGACACGCGCGACAACAGTTTCATTGTTGCTCGTGATGCAATTGGAATTACTTCCCTTTACATTGGTTGGGGACTCGATGGCTCAGTTTGGATATCTTCAGAACTCAAAGGTCTGAATGATGATTGTGAACATTTTGAGACATTTCCACCGGGTCACTTGTACTCTAGCAAGGACGCTAGATTCAAAAGATGGTATAATCCAACCTGGTTCTCCGAGGATATTCCATCGACCCCATATGACCCACTTGTTCTGAGACGCGCATTGAAGAATGCTGTGATCAAGAGGCTGATGACCGATGTCCCTTTTGGAGTTCTGCTATCTGGTGGTCTAGATTCATCATTGGTTGCCTCTATCACAGCTCGTTACTTAGCTGGCACAAGAGCTGCCAAGCATTGGGGTACACAACTTCATTCCTTCTGTGTTGGTCTTGAGGGATCACCAGATTTGAAGGCTGCTAGAGAAGTTGCAGACTTTTTAGGAACTGTTCACCACGAGTTCCATTTCACAGTTCAGGATGGGATTGATGCCATTGAAGATGTTATCTACCATATAGAAACATATGATGTAACTACAATCAGGGCAAGCACACCCATGTTTCTTATGGCACGAAAGATTAAGTCACTAGGAGTAAAGATGGTGATTTCTGGTGAAGGCGCTGATGAAATTTTTGGCGGATACCTGTATTTCCACAAGGCTCCTAATAAGGAAGAGTTTCACTGTGAAAGTTGTCGCAAGATCAAACGTGATCAAGGCCGAATTGAAAAATGGGTTCTCAGGAGAGCTTTTGATGATGAACACCAACCTTATCTCCCTAAGCATGTTTTGTACAGGCAAAAGGAACAATTCAGTGATGGTGTTGGATATAGTTGGATTGATGGTCTTAAGGCCCATGCTGCTCAACACGTGACTGATAAAATGATGTTGAACGCTGAACACATCTTCCCTCAAAATACTCCAACATCAAAGGAAGCCTACTATTATAGAACCATATTTGAGAGGTTTTTCCCACAGAACTCAGCTCGTCTGACTGTCCCGGGAGGACCAAGTATCGCTTGTAGTACGGCAAAGGCTGTCGAATGGGATGCTGCTTGGTCTAAAAATCTTGATCCTTCAGGTAGAGCTGCACTTGGTGTTCATGCCTCAGCATATGGAGAGGAGACAAATAGTGTAAGCGACGTTGGCCCGCCCAAGATAATCGATACCATTTCGAGAATGGAGGTCAATACACCAGGAGTAAAAATCCTTAGCTAG

Protein sequence

MILKLNVFVFLNSLVGKCSQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVTYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTPYDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIKRDQGRIEKWVLRRAFDDEHQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDVGPPKIIDTISRMEVNTPGVKILS
BLAST of Cla021971 vs. Swiss-Prot
Match: ASNS_ASPOF (Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis PE=2 SV=2)

HSP 1 Score: 751.1 bits (1938), Expect = 7.7e-216
Identity = 380/564 (67.38%), Postives = 420/564 (74.47%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYN------------------- 78
           S+ LKHRGPDWSGL QH D +L+HQRLAIIDPASGDQPLYN                   
Sbjct: 25  SRRLKHRGPDWSGLCQHGDCFLSHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEEL 84

Query: 79  -------------EDKSIVVTYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        + + I   YEE+GE+FVDMLDGMFSFVLLDTR+N F+ ARDA+GIT 
Sbjct: 85  RRRLPDHKYRTGSDCEVIAHLYEEHGEDFVDMLDGMFSFVLLDTRNNCFVAARDAVGITP 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVW+SSE+KGLNDDCEHFE FPPG+LYSS+   F+RWYNP W++E IPS P
Sbjct: 145 LYIGWGLDGSVWLSSEMKGLNDDCEHFEVFPPGNLYSSRSGSFRRWYNPQWYNETIPSAP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLR+A ++AVIKRLMTDVPFGVLLSGGLDSSLVA++TAR+LAG++AA+ WGTQLHS
Sbjct: 205 YDPLVLRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAA+EVA++LGTVHHEFHFTVQDGIDAIEDVI+HIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIFHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLMARKIKSLGVKMVISGEG+DEIFGGYLYFHKAPNKEEFH E+CRKIK        R  
Sbjct: 325 FLMARKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHHETCRKIKALHQYDCLRAN 384

Query: 379 GRIEKWVLRRAF-------------------------------------DDEHQPYLPKH 438
                W L                                         DDE  PYLPKH
Sbjct: 385 KATSAWGLEARVPFLDKEFMDVAMSIDPESKMIKPDLGRIEKWVLRKAFDDEENPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           +LYRQKEQFSDGVGYSWIDGLKAHAA+HVTD+MMLNA  I+P NTPT+KEAYYYR IFER
Sbjct: 445 ILYRQKEQFSDGVGYSWIDGLKAHAAKHVTDRMMLNAARIYPHNTPTTKEAYYYRMIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 503
           FFPQNSAR TVPGGPSIACSTAKA+EWDA WS NLDPSGRAALGVH SAY     S    
Sbjct: 505 FFPQNSARFTVPGGPSIACSTAKAIEWDARWSNNLDPSGRAALGVHDSAYDPPLPSSISA 564

BLAST of Cla021971 vs. Swiss-Prot
Match: ASNS_TRIVS (Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor GN=AS PE=2 SV=3)

HSP 1 Score: 705.3 bits (1819), Expect = 4.8e-202
Identity = 363/530 (68.49%), Postives = 396/530 (74.72%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------------ 78
           S+ LKHRGPDWSG++ H D YLAHQRLAI+DPASGDQPL+NE                  
Sbjct: 25  SRRLKHRGPDWSGIHHHGDCYLAHQRLAIVDPASGDQPLFNEDKRIAVTVNGEIYNHEEL 84

Query: 79  -------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        D  ++   YEEYGENFV+MLDGMFSFVLLD+RDN+FI ARDA GITS
Sbjct: 85  RALLPNHKFRTGSDCDVIAHLYEEYGENFVEMLDGMFSFVLLDSRDNTFIAARDAFGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSELKGL+D+CE+FE FPPGH+YSSK   F+RWYNP WFSE IPSTP
Sbjct: 145 LYIGWGLDGSVWISSELKGLHDECENFEVFPPGHVYSSKTEGFRRWYNPPWFSEAIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLR A + AVIKRLMTDVPFGVLLSGGLDSSLVA++TAR+LAGT+AAK WG+QLHS
Sbjct: 205 YDPLVLRGAFEQAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGTKAAKRWGSQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKA +EVAD+LGTVHHEF FTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAGKEVADYLGTVHHEFLFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLM+RKIKSLGVKMVISGEG+DEIFGGYLYFHKAPNKEEFH E+CRKIK        R  
Sbjct: 325 FLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALHQYDCLRAN 384

Query: 379 GRIEKWVL--RRAFDDEH------------------QPYLPKHVLYRQKEQFS------- 438
                W L  R  F D+                   Q  + K +L +  +          
Sbjct: 385 KATSAWGLEARVPFLDKEFVNLAMSIDPEAKMIKPDQGRIEKWILRKAFDDEERPYLPKH 444

Query: 439 ----------DGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 472
                     DGVGYSWIDGLKAHA QHVTDKMMLNA HIFP NTPT+KE YYYR IFER
Sbjct: 445 ILYRQKEQFSDGVGYSWIDGLKAHAEQHVTDKMMLNAGHIFPHNTPTTKEGYYYRMIFER 504

BLAST of Cla021971 vs. Swiss-Prot
Match: ASNS2_ORYSJ (Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica GN=Os06g0265000 PE=2 SV=3)

HSP 1 Score: 669.5 bits (1726), Expect = 2.9e-191
Identity = 338/553 (61.12%), Postives = 402/553 (72.69%), Query Frame = 1

Query: 13  SLVGKCSQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------ 72
           S + + S+ L+HRGPDWSG++ + D YLAHQRLAI+DP SGDQPLYNE            
Sbjct: 19  SRIIELSRRLRHRGPDWSGIHCYQDCYLAHQRLAIVDPTSGDQPLYNEDKSVVVTVNGEI 78

Query: 73  -------------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARD 132
                              D  ++   YEEYGE FVDMLDGMF+FVLLDTRD SFI ARD
Sbjct: 79  YNHEELKANLKSHKFQTASDCEVIAHLYEEYGEEFVDMLDGMFAFVLLDTRDKSFIAARD 138

Query: 133 AIGITSLYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSE 192
           AIGI  LY+GWGLDGSVW SSE+K L+DDCE F +FPPGHLYSSK    +RWYNP WFSE
Sbjct: 139 AIGICPLYMGWGLDGSVWFSSEMKALSDDCERFISFPPGHLYSSKTGGLRRWYNPPWFSE 198

Query: 193 DIPSTPYDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHW 252
            IPSTPY+PL+LR++ + A+IKRLMTDVPFGVLLSGGLDSSLVAS+ +R+LA  + A  W
Sbjct: 199 SIPSTPYNPLLLRQSFEKAIIKRLMTDVPFGVLLSGGLDSSLVASVVSRHLAEAKVAAQW 258

Query: 253 GTQLHSFCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTI 312
           G +LH+FC+GL+GSPDL+AA+EVAD+LGTVHHE HFTVQ+GIDA+E+VIYH+ETYDVTTI
Sbjct: 259 GNKLHTFCIGLKGSPDLRAAKEVADYLGTVHHELHFTVQEGIDALEEVIYHVETYDVTTI 318

Query: 313 RASTPMFLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIKR---- 372
           RASTPMFLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPNK+EFH E+CRKIK     
Sbjct: 319 RASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKKEFHEETCRKIKALHLY 378

Query: 373 -------------DQGRI---EKWVLRRAFDDEHQPYLPKHVLYRQKE------------ 432
                         + R+   +K  +  A D + +  + K  L R ++            
Sbjct: 379 DCLRANKSTSAWGVEARVPFLDKNFINVAMDIDPEWKMIKRDLGRIEKWVLRNAFDDEEK 438

Query: 433 -------------QFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYY 489
                        QFSDGVGYSWIDGLK HA +HV+D MM+NA  ++P+NTP +KEAYYY
Sbjct: 439 PYLPKHILYRQKEQFSDGVGYSWIDGLKDHANEHVSDSMMMNASFVYPENTPVTKEAYYY 498

BLAST of Cla021971 vs. Swiss-Prot
Match: ASNS1_LOTJA (Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus GN=AS1 PE=2 SV=2)

HSP 1 Score: 564.7 bits (1454), Expect = 1.0e-159
Identity = 272/349 (77.94%), Postives = 298/349 (85.39%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSGL+QH D YLAHQRLAI+DPASGDQPL+NEDKSI+VT           
Sbjct: 25  SRRLKHRGPDWSGLHQHGDCYLAHQRLAIVDPASGDQPLFNEDKSIIVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEE+GENF+DMLDG+FSFVLLDTRDN+FIVARDAIG+TS
Sbjct: 85  RKQLPNHQFRTGSDCDVIAHLYEEHGENFMDMLDGIFSFVLLDTRDNTFIVARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSE+KGLNDDCEHFE FPPGHLYSS++  F+RWYNPTWFSE IPS P
Sbjct: 145 LYIGWGLDGSVWISSEMKGLNDDCEHFEVFPPGHLYSSRERAFRRWYNPTWFSESIPSAP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPL +R A + AVIKRLMTDVPFGVLLSGGLDSSLVASIT+RYLA T+AA+ WG++LHS
Sbjct: 205 YDPLAVRHAFEKAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLATTKAAEQWGSKLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAA+EVAD+LGTVHHEF FTVQDGIDAIE+VIYH+ETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAKEVADYLGTVHHEFTFTVQDGIDAIEEVIYHVETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK 336
           FLM+RKIKSLGVK VISGEG+DEIFGGYLYFHKAPNKEEFH E+CRKIK
Sbjct: 325 FLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAPNKEEFHTETCRKIK 373


HSP 2 Score: 261.2 bits (666), Expect = 2.4e-68
Identity = 126/173 (72.83%), Postives = 144/173 (83.24%), Query Frame = 1

Query: 332 RKIKRDQGRIEKWVLRRAFDDEHQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVT 391
           + IKRD+GRIEK++LRRAFDDE +PYLPKH+LYRQKEQFSDGVGYSWIDGLK HAA+HVT
Sbjct: 415 KMIKRDEGRIEKYILRRAFDDEEKPYLPKHILYRQKEQFSDGVGYSWIDGLKDHAAKHVT 474

Query: 392 DKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAKAVEWDAA 451
           DKM+LNA +IF  NTP +KEAYYYR IFERFFPQNSARLTVPGGP++ACSTAKAVEWDAA
Sbjct: 475 DKMILNAGNIFRHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGPTVACSTAKAVEWDAA 534

Query: 452 WSKNLDPSGRAALGVHASAYGEETNSVSDVGPPKIIDTISRMEVNTPGVKILS 505
           WS NLDPSGRAALGVH SAY ++ N++ +   P   + +  ME  + GV I S
Sbjct: 535 WSNNLDPSGRAALGVHLSAYDDKQNNLIN-NKPVEFEKLIPMEAPSLGVAIHS 586

BLAST of Cla021971 vs. Swiss-Prot
Match: ASNS_SANAU (Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca GN=AND1 PE=2 SV=3)

HSP 1 Score: 555.4 bits (1430), Expect = 6.2e-157
Identity = 268/349 (76.79%), Postives = 294/349 (84.24%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSGL  H D YLAHQRLAIIDPASGDQPLYNEDK+I+VT           
Sbjct: 25  SRRLKHRGPDWSGLDHHGDCYLAHQRLAIIDPASGDQPLYNEDKTIIVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEE+GE+F+ MLDG+FSFVLLD+R+NSF+ ARDAIG+T 
Sbjct: 85  RKGLPGHTFRTGSDCEVIAHLYEEHGESFIHMLDGIFSFVLLDSRNNSFVAARDAIGVTP 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSE+KGLNDDCEHF+ FPPGHLYSSK+  FKRWYNP WFSE IPS P
Sbjct: 145 LYIGWGLDGSVWISSEMKGLNDDCEHFKFFPPGHLYSSKEGSFKRWYNPPWFSEVIPSVP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           +DPL LR+A ++AVIKRLMTDVPFGVLLSGGLDSSLVAS+TARYL GT+AA+ WGTQLHS
Sbjct: 205 FDPLALRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASVTARYLEGTKAAELWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAA+EVA+FLGT+HHEFHFTVQDGIDAIEDVIYH+ETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAKEVANFLGTIHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK 336
           FLM+RKIKSLGVKMVISGEG+DEIFGGYLYFHKAPNKEE H E+C KIK
Sbjct: 325 FLMSRKIKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEELHLETCHKIK 373


HSP 2 Score: 174.9 bits (442), Expect = 2.3e-42
Identity = 79/94 (84.04%), Postives = 83/94 (88.30%), Query Frame = 1

Query: 332 RKIKRDQGRIEKWVLRRAFDDEHQPYLPKHVLYRQKEQFSDGVGYSWIDGLKAHAAQHVT 391
           + IK D GRIEKW+LRRAFDDE  PYLPKH+LYRQKEQFSDGVGYSWIDGLKAH+A HVT
Sbjct: 415 KMIKPDIGRIEKWILRRAFDDEENPYLPKHILYRQKEQFSDGVGYSWIDGLKAHSALHVT 474

Query: 392 DKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQ 426
           DKMMLNA HI+P NTPT+KEAYYYR IFERFFPQ
Sbjct: 475 DKMMLNAAHIYPHNTPTTKEAYYYRMIFERFFPQ 508

BLAST of Cla021971 vs. TrEMBL
Match: A0A0A0KF94_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G183190 PE=4 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 3.6e-252
Identity = 446/563 (79.22%), Postives = 461/563 (81.88%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------------ 78
           S+ LKHRGPDWSGLYQH D YLAHQRLAIIDPASGDQPLYNE                  
Sbjct: 25  SRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGDQPLYNEDQSVVVTVNGEIYNHEEL 84

Query: 79  -------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        D  ++   YEEYGENFVDMLDGMFSFVLLDTRDNSF+VARDAIGITS
Sbjct: 85  RKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFVVARDAIGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A+FKRWYNPTWFSEDIPSTP
Sbjct: 145 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAKFKRWYNPTWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRA +NAVIKRLMTDVPFGVLLSGGLDSSLVASITAR+L GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           +LMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 YLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRRA-------------------------------------FDDEHQPYLPKH 438
                W L                                        FDDE QPYLPKH
Sbjct: 385 KATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRDQGRIEKWVLRRAFDDEQQPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 505
           FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHA+AYG+ETN V++V
Sbjct: 505 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHAAAYGDETNGVNNV 564

BLAST of Cla021971 vs. TrEMBL
Match: A0A0A0KD27_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G362920 PE=4 SV=1)

HSP 1 Score: 795.8 bits (2054), Expect = 3.0e-227
Identity = 404/512 (78.91%), Postives = 414/512 (80.86%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSGLYQHAD YL+HQRLAI+DPASGDQPLYNE+KSIVVT           
Sbjct: 25  SRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSIVVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEEYGENFVDMLDGMFSFVLLDTRDNSFI ARDAIG+TS
Sbjct: 85  RKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFIAARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A FKRWYNP WFSEDIPSTP
Sbjct: 145 LYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRWYNPIWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRAL+NAVIKRLMTDVPFGVLLSGGLDSSLVASITARYL GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 FLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRR-------------------------------------AFDDEHQPYLPKH 438
                W L                                       AFDDE QPYLPK+
Sbjct: 385 KATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKN 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 454
           VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFER 504

BLAST of Cla021971 vs. TrEMBL
Match: A0A067LBF0_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17398 PE=4 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.1e-224
Identity = 400/565 (70.80%), Postives = 434/565 (76.81%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------------ 78
           S+ LKHRGPDWSGLYQ  DFYLAHQRLAIIDPASGDQPL+NE                  
Sbjct: 25  SRRLKHRGPDWSGLYQQGDFYLAHQRLAIIDPASGDQPLFNEDKAIVVTVNGEIYNHEEL 84

Query: 79  -------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        D  ++   YEEYGENFVDMLDGMFSFV+LDTRDNS+IVARDAIGITS
Sbjct: 85  RKRLPNHKFRTGSDCDVIAHLYEEYGENFVDMLDGMFSFVILDTRDNSYIVARDAIGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGS+WI+SELKGLNDDCEHFE+FPPGHLYSSK    + WYNP WFS+ IPSTP
Sbjct: 145 LYIGWGLDGSIWIASELKGLNDDCEHFESFPPGHLYSSKSGGLRPWYNPPWFSDVIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPL LRRA +NAV+KRLMTDVPFGVLLSGGLDSSLVASITAR+LAGT+AAK WG+QLHS
Sbjct: 205 YDPLALRRAFENAVVKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGSQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAA+EVAD+LGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLM+RKIK+LGVKMV+SGEG+DE+FGGYLYFHKAPNKEEFH E+CRKIK        R  
Sbjct: 325 FLMSRKIKALGVKMVLSGEGSDEVFGGYLYFHKAPNKEEFHRETCRKIKALHQYDCLRAN 384

Query: 379 GRIEKWVLRRA-------------------------------------FDDEHQPYLPKH 438
                W L                                        FDDE QPYLPKH
Sbjct: 385 KATSAWGLEARVPFLDKEFINVAMAIDPEWKMIKPEQGRIEKWVLRRAFDDEEQPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           +LYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMM NA HIFP NTPT+KEAYYYR IFER
Sbjct: 445 ILYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMQNAGHIFPHNTPTTKEAYYYRMIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSD- 505
           FFPQNSARLTVPGG S+ACSTAKAVEWDA WS NLDPSGRAALGVH +AY  +  + +  
Sbjct: 505 FFPQNSARLTVPGGASVACSTAKAVEWDATWSNNLDPSGRAALGVHLAAYDPQAAAANPA 564

BLAST of Cla021971 vs. TrEMBL
Match: A0A0K9RGQ2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_068530 PE=4 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 4.5e-215
Identity = 389/564 (68.97%), Postives = 428/564 (75.89%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSG+YQ+ D YL+HQRLAIIDPASGDQPLYNE K+IVVT           
Sbjct: 25  SRRLKHRGPDWSGIYQNGDCYLSHQRLAIIDPASGDQPLYNESKTIVVTVNGEIYNHEVL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEE+GENF+DMLDG+FSFVLLDTRDNS+I ARDAIGITS
Sbjct: 85  RKSLPNHKFRTGSDCDVIAHLYEEHGENFIDMLDGVFSFVLLDTRDNSYIAARDAIGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSELKGL+DDCEHFE+FPPGHLYSSK   F+RWYNP WFSE IPS  
Sbjct: 145 LYIGWGLDGSVWISSELKGLHDDCEHFESFPPGHLYSSKTGSFRRWYNPLWFSEAIPSVT 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPL+LR+A ++AVIKRLMTDVPFGVLLSGGLDSSLVASIT+R+LAGT+AA+ WG+QLHS
Sbjct: 205 YDPLILRKAFEDAVIKRLMTDVPFGVLLSGGLDSSLVASITSRHLAGTKAARQWGSQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDL AAREVAD+LGTVHHEF+FTVQDGIDAI+DVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLVAAREVADYLGTVHHEFNFTVQDGIDAIDDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLM+RKIKSLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFH E+CRKIK        R  
Sbjct: 325 FLMSRKIKSLGVKMVMSGEGADEIFGGYLYFHKAPNKEEFHQETCRKIKALHLYDCLRAN 384

Query: 379 GRIEKWVL--RRAFDDEH-----------------------------------QPYLPKH 438
                W L  R  F D+                                    QPYLPKH
Sbjct: 385 KSTSAWGLEARVPFLDKEFIDVAMSIDPEWKMIKPEERRLEKWILRKAFDDEKQPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           +LYRQKEQFSDGVGYSWIDGLKAHA Q VTDKMM  AEHIFP NTP +KEA YYR IFER
Sbjct: 445 ILYRQKEQFSDGVGYSWIDGLKAHAEQQVTDKMMQQAEHIFPHNTPKTKEACYYRMIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 505
            FPQNSARLTVPGGPS+ACSTAKA+EWD +WS NLDPSGRAA GVH SAY  + +  +  
Sbjct: 505 LFPQNSARLTVPGGPSVACSTAKAIEWDQSWSTNLDPSGRAAHGVHNSAYERQPSGSNIN 564

BLAST of Cla021971 vs. TrEMBL
Match: V7BCL1_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G259900g PE=4 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 2.9e-214
Identity = 388/558 (69.53%), Postives = 425/558 (76.16%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKS--------------- 78
           S+ LKHRGPDWSGL+QH D +LAHQRLAI+DPASGDQPL+NEDKS               
Sbjct: 25  SRRLKHRGPDWSGLHQHGDCFLAHQRLAIVDPASGDQPLFNEDKSVIVTVNGEIYNHEEL 84

Query: 79  -----------------IVVTYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                            I   YEE+GE FVDMLDG+FSFVLLDTRDNSFIVARDAIG+TS
Sbjct: 85  RKQLPGHKFRTGSDCDVIAHLYEEHGEGFVDMLDGIFSFVLLDTRDNSFIVARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSE+KGLNDDCEHFE FPPGHLYSSKD  F+RWYNP WFSE IPS P
Sbjct: 145 LYIGWGLDGSVWISSEMKGLNDDCEHFECFPPGHLYSSKDRGFRRWYNPPWFSEAIPSAP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRA + AVIKRLMTDVPFGVLLSGGLDSSLVASIT+RYLA T+AA+ WG++LHS
Sbjct: 205 YDPLVLRRAFEQAVIKRLMTDVPFGVLLSGGLDSSLVASITSRYLANTKAAEQWGSKLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAA+EVAD+LGTVHHEFHFTVQDGIDAIEDVIYH+ETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNK--EEFHCESCRKIKRDQ------ 378
           FLM+RKIKSLGVK VISGEG+DEIFGGYLYFHKA     +  H   C +  +        
Sbjct: 325 FLMSRKIKSLGVKWVISGEGSDEIFGGYLYFHKAHRGIIKALHQYDCLRANKSTFAWGLE 384

Query: 379 ----------------------------GRIEKWVLRRAF----DDEHQPYLPKHVLYRQ 438
                                       GRIEKW+         DDE QPYLPKH+LYRQ
Sbjct: 385 ARVPFLDKEFINAAMNIDPEHKMIKRDEGRIEKWI----LRRAFDDEDQPYLPKHILYRQ 444

Query: 439 KEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQN 498
           KEQFSDGVGYSWIDGLKAHAA+HVTDKMMLNA +I+P NTP +KEAYYYR IFERFFPQN
Sbjct: 445 KEQFSDGVGYSWIDGLKAHAAKHVTDKMMLNAGNIYPHNTPVTKEAYYYRMIFERFFPQN 504

Query: 499 SARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDVGPPKI 505
           SARLTVPGG S+ACSTAKAVEWDAAWS NLDPSGRAALGVH SAY E  N++++ G    
Sbjct: 505 SARLTVPGGASVACSTAKAVEWDAAWSNNLDPSGRAALGVHISAY-ENQNNLANKGVE-- 564

BLAST of Cla021971 vs. NCBI nr
Match: gi|449450826|ref|XP_004143163.1| (PREDICTED: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 878.6 bits (2269), Expect = 5.1e-252
Identity = 446/563 (79.22%), Postives = 461/563 (81.88%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------------ 78
           S+ LKHRGPDWSGLYQH D YLAHQRLAIIDPASGDQPLYNE                  
Sbjct: 25  SRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGDQPLYNEDQSVVVTVNGEIYNHEEL 84

Query: 79  -------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        D  ++   YEEYGENFVDMLDGMFSFVLLDTRDNSF+VARDAIGITS
Sbjct: 85  RKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFVVARDAIGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A+FKRWYNPTWFSEDIPSTP
Sbjct: 145 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAKFKRWYNPTWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRA +NAVIKRLMTDVPFGVLLSGGLDSSLVASITAR+L GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           +LMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 YLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRRA-------------------------------------FDDEHQPYLPKH 438
                W L                                        FDDE QPYLPKH
Sbjct: 385 KATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRDQGRIEKWVLRRAFDDEQQPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 505
           FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHA+AYG+ETN V++V
Sbjct: 505 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHAAAYGDETNGVNNV 564

BLAST of Cla021971 vs. NCBI nr
Match: gi|659130620|ref|XP_008465262.1| (PREDICTED: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 872.1 bits (2252), Expect = 4.8e-250
Identity = 444/563 (78.86%), Postives = 459/563 (81.53%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNE------------------ 78
           S+ LKHRGPDWSGLYQH D YLAHQRLAIIDPASGDQPLYNE                  
Sbjct: 25  SRRLKHRGPDWSGLYQHKDCYLAHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEEL 84

Query: 79  -------------DKSIVV-TYEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                        D  ++   YEEYGENFVDMLDGMFSFVLLDTRDNSF+VARDAIGITS
Sbjct: 85  RKKLRNHKIRTGSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFVVARDAIGITS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A+FKRWYNPTWFSEDIPSTP
Sbjct: 145 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAKFKRWYNPTWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRAL+NAVIKRLMTDVPFGVLLSGGLDSSLVASITAR+L GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           +LMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 YLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRRA-------------------------------------FDDEHQPYLPKH 438
                W L                                        FDDE QPYLPKH
Sbjct: 385 KATSAWGLEVRVPFLDKEFINVAMAIDPEWKMIKRDQGRIEKWVLRRAFDDEQQPYLPKH 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 505
           FFPQNSARLTVPGGPSIACSTAKA+EWDAAWSKNLDPSGRAALGVHA+AYG+E N V ++
Sbjct: 505 FFPQNSARLTVPGGPSIACSTAKAIEWDAAWSKNLDPSGRAALGVHAAAYGDEANGV-NI 564

BLAST of Cla021971 vs. NCBI nr
Match: gi|778715252|ref|XP_004144212.2| (PREDICTED: LOW QUALITY PROTEIN: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 863.2 bits (2229), Expect = 2.2e-247
Identity = 440/563 (78.15%), Postives = 456/563 (80.99%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSGLYQHAD YL+HQRLAI+DPASGDQPLYNE+KSIVVT           
Sbjct: 25  SRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSIVVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEEYGENFVDMLDGMFSFVLLDTRDNSFI ARDAIG+TS
Sbjct: 85  RKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFIAARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A FKRWYNP WFSEDIPSTP
Sbjct: 145 LYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRWYNPIWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRAL+NAVIKRLMTDVPFGVLLSGGLDSSLVASITARYL GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 FLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRR-------------------------------------AFDDEHQPYLPKH 438
                W L                                       AFDDE QPYLPK+
Sbjct: 385 KATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKN 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 498
           VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFER 504

Query: 499 FFPQNSARLTVPGGPSIACSTAKAVEWDAAWSKNLDPSGRAALGVHASAYGEETNSVSDV 505
           FFPQNSA LTVPGGPSIACSTAKAVEWDAAWS NL PS RAALGVHAS YG+E N ++D+
Sbjct: 505 FFPQNSACLTVPGGPSIACSTAKAVEWDAAWSXNLGPS-RAALGVHASTYGDEANGLNDI 564

BLAST of Cla021971 vs. NCBI nr
Match: gi|700192381|gb|KGN47585.1| (hypothetical protein Csa_6G362920 [Cucumis sativus])

HSP 1 Score: 795.8 bits (2054), Expect = 4.3e-227
Identity = 404/512 (78.91%), Postives = 414/512 (80.86%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ LKHRGPDWSGLYQHAD YL+HQRLAI+DPASGDQPLYNE+KSIVVT           
Sbjct: 25  SRRLKHRGPDWSGLYQHADCYLSHQRLAIVDPASGDQPLYNENKSIVVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEEYGENFVDMLDGMFSFVLLDTRDNSFI ARDAIG+TS
Sbjct: 85  RKKLRNHKIRTCSDCDVISHLYEEYGENFVDMLDGMFSFVLLDTRDNSFIAARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWG DGSVWISSELKGLNDDCEHFETFPPGHLYSSK+A FKRWYNP WFSEDIPSTP
Sbjct: 145 LYIGWGRDGSVWISSELKGLNDDCEHFETFPPGHLYSSKEAGFKRWYNPIWFSEDIPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           YDPLVLRRAL+NAVIKRLMTDVPFGVLLSGGLDSSLVASITARYL GTRAAKHWGTQLHS
Sbjct: 205 YDPLVLRRALENAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLTGTRAAKHWGTQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIK--------RDQ 378
           FLMARKIKSLGVKMV+SGEGADEIFGGYLYFHKAPNKEEFH ESCRKIK        R  
Sbjct: 325 FLMARKIKSLGVKMVVSGEGADEIFGGYLYFHKAPNKEEFHRESCRKIKALHMYDCLRAN 384

Query: 379 GRIEKWVLRR-------------------------------------AFDDEHQPYLPKH 438
                W L                                       AFDDE QPYLPK+
Sbjct: 385 KATSAWGLEVRVPFLDKKFIDVAMAIDPEWKMIKHNQGRIEKWVLRRAFDDEQQPYLPKN 444

Query: 439 VLYRQKEQFSDGVGYSWIDGLKAHAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFER 454
           VLYRQKEQFSDGVGYSWIDGLKAHA QHVTDKM+LNAEHIFPQNTPTSKEAYYYRTIFER
Sbjct: 445 VLYRQKEQFSDGVGYSWIDGLKAHADQHVTDKMILNAEHIFPQNTPTSKEAYYYRTIFER 504

BLAST of Cla021971 vs. NCBI nr
Match: gi|923746447|ref|XP_013672828.1| (PREDICTED: asparagine synthetase [glutamine-hydrolyzing])

HSP 1 Score: 790.0 bits (2039), Expect = 2.4e-225
Identity = 382/526 (72.62%), Postives = 431/526 (81.94%), Query Frame = 1

Query: 19  SQSLKHRGPDWSGLYQHADFYLAHQRLAIIDPASGDQPLYNEDKSIVVT----------- 78
           S+ L+HRGPDWSG+YQH D YLAH+RLAI+DP SGDQPL+NEDK+IVVT           
Sbjct: 25  SRRLRHRGPDWSGIYQHEDNYLAHERLAIVDPDSGDQPLFNEDKTIVVTVNGEIYNHEEL 84

Query: 79  ---------------------YEEYGENFVDMLDGMFSFVLLDTRDNSFIVARDAIGITS 138
                                YEE+GENFVDMLDG+FSFVLLDT+ NSF+VARDAIG+TS
Sbjct: 85  RKDLKNHKFRTGSDCDVISHLYEEHGENFVDMLDGVFSFVLLDTKHNSFMVARDAIGVTS 144

Query: 139 LYIGWGLDGSVWISSELKGLNDDCEHFETFPPGHLYSSKDARFKRWYNPTWFSEDIPSTP 198
           LYIGWGLDGSVWISSE+KGL++DCEHFE FPPGHLYSSK  RFK+WYNP W++E +PSTP
Sbjct: 145 LYIGWGLDGSVWISSEMKGLHEDCEHFEVFPPGHLYSSKSGRFKQWYNPPWYNESVPSTP 204

Query: 199 YDPLVLRRALKNAVIKRLMTDVPFGVLLSGGLDSSLVASITARYLAGTRAAKHWGTQLHS 258
           Y+PL +RRA ++AV KRLM+DVPFGVLLSGGLDSSLV SITAR+LAGT+AAK WG+QLHS
Sbjct: 205 YEPLAVRRAFEDAVTKRLMSDVPFGVLLSGGLDSSLVVSITARHLAGTKAAKRWGSQLHS 264

Query: 259 FCVGLEGSPDLKAAREVADFLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPM 318
           FCVGLEGSPD+KA +EVAD+LGTVHHEFHFTVQDGIDAIEDVIYH+ETYDVTTIRASTPM
Sbjct: 265 FCVGLEGSPDVKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPM 324

Query: 319 FLMARKIKSLGVKMVISGEGADEIFGGYLYFHKAPNKEEFHCESCRKIKR---------- 378
           FLM+RKIKSLGVKMV+SGEG+DEIFGGYLYFHKAPNK+EFH E+CRKIK           
Sbjct: 325 FLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKQEFHQETCRKIKALHKYDCLRAN 384

Query: 379 ------------DQGRIEKWVLRRAFDDEHQPYLPKHVLYRQKEQFSDGVGYSWIDGLKA 438
                       ++GRIEKWVLRRAFDDE +PYLPKH+LYRQKEQFSDGVGYSWIDGLKA
Sbjct: 385 KATSAFGLEIKPEEGRIEKWVLRRAFDDEERPYLPKHILYRQKEQFSDGVGYSWIDGLKA 444

Query: 439 HAAQHVTDKMMLNAEHIFPQNTPTSKEAYYYRTIFERFFPQNSARLTVPGGPSIACSTAK 490
           HAA++V DKMM NA HIFP NTP +KEAYYYR IFERFFPQNSARLTVPGG ++ACSTAK
Sbjct: 445 HAAENVNDKMMSNAAHIFPHNTPLTKEAYYYRMIFERFFPQNSARLTVPGGATVACSTAK 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
ASNS_ASPOF7.7e-21667.38Asparagine synthetase [glutamine-hydrolyzing] OS=Asparagus officinalis PE=2 SV=2[more]
ASNS_TRIVS4.8e-20268.49Asparagine synthetase [glutamine-hydrolyzing] OS=Triphysaria versicolor GN=AS PE... [more]
ASNS2_ORYSJ2.9e-19161.12Asparagine synthetase [glutamine-hydrolyzing] 2 OS=Oryza sativa subsp. japonica ... [more]
ASNS1_LOTJA1.0e-15977.94Asparagine synthetase [glutamine-hydrolyzing] 1 OS=Lotus japonicus GN=AS1 PE=2 S... [more]
ASNS_SANAU6.2e-15776.79Asparagine synthetase [glutamine-hydrolyzing] OS=Sandersonia aurantiaca GN=AND1 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KF94_CUCSA3.6e-25279.22Uncharacterized protein OS=Cucumis sativus GN=Csa_6G183190 PE=4 SV=1[more]
A0A0A0KD27_CUCSA3.0e-22778.91Uncharacterized protein OS=Cucumis sativus GN=Csa_6G362920 PE=4 SV=1[more]
A0A067LBF0_JATCU1.1e-22470.80Uncharacterized protein OS=Jatropha curcas GN=JCGZ_17398 PE=4 SV=1[more]
A0A0K9RGQ2_SPIOL4.5e-21568.97Uncharacterized protein OS=Spinacia oleracea GN=SOVF_068530 PE=4 SV=1[more]
V7BCL1_PHAVU2.9e-21469.53Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_008G259900g PE=4 SV=1[more]
Match NameE-valueIdentityDescription
gi|449450826|ref|XP_004143163.1|5.1e-25279.22PREDICTED: asparagine synthetase [glutamine-hydrolyzing][more]
gi|659130620|ref|XP_008465262.1|4.8e-25078.86PREDICTED: asparagine synthetase [glutamine-hydrolyzing][more]
gi|778715252|ref|XP_004144212.2|2.2e-24778.15PREDICTED: LOW QUALITY PROTEIN: asparagine synthetase [glutamine-hydrolyzing][more]
gi|700192381|gb|KGN47585.1|4.3e-22778.91hypothetical protein Csa_6G362920 [Cucumis sativus][more]
gi|923746447|ref|XP_013672828.1|2.4e-22572.62PREDICTED: asparagine synthetase [glutamine-hydrolyzing][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001962Asn_synthase
IPR014729Rossmann-like_a/b/a_fold
IPR017932GATase_2_dom
Vocabulary: Molecular Function
TermDefinition
GO:0004066asparagine synthase (glutamine-hydrolyzing) activity
Vocabulary: Biological Process
TermDefinition
GO:0006529asparagine biosynthetic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006522 alanine metabolic process
biological_process GO:0006529 asparagine biosynthetic process
biological_process GO:0006531 aspartate metabolic process
biological_process GO:0009063 cellular amino acid catabolic process
biological_process GO:0009646 response to absence of light
cellular_component GO:0005575 cellular_component
molecular_function GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla021971Cla021971.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001962Asparagine synthasePFAMPF00733Asn_synthasecoord: 172..324
score: 2.1E-56coord: 338..428
score: 2.8
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 338..427
score: 1.4E-21coord: 152..337
score: 1.9
IPR017932Glutamine amidotransferase type 2 domainPFAMPF13537GATase_7coord: 67..127
score: 9.1
IPR017932Glutamine amidotransferase type 2 domainPROFILEPS51278GATASE_TYPE_2coord: 1..147
score: 10
NoneNo IPR availablePANTHERPTHR11772ASPARAGINE SYNTHETASEcoord: 19..455
score: 8.4E
NoneNo IPR availablePANTHERPTHR11772:SF13ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING] 2-RELATEDcoord: 19..455
score: 8.4E
NoneNo IPR availableunknownSSF52402Adenine nucleotide alpha hydrolases-likecoord: 171..397
score: 4.42