Cla021897 (gene) Watermelon (97103) v1

NameCla021897
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPolygalacturonase (AHRD V1 ***- E3VSV7_CUCPE); contains Interpro domain(s) IPR000743 Glycoside hydrolase, family 28
LocationChr8 : 18317715 .. 18319209 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTACAACCTTCAACTTTGTGGCTCTATTATTTTTTATATTATTTTTTGTCTCCAGAGGCCAAACCCAATCCAACGACGGTGTTTTCGATGTGACAAAGTATGGTGCTAAGCCTAATGTTGATATATCTAAGGTAAACTCAATTTTTTCTTTTTTTAATATCATCCATCGAAAGGAGGAACATCTAGTGACATAATTTATTTTTTAATATATATTTTGAAAATTAAATAGGCTCTAACAAGTGCATGGAAGGAAGCTTGTGCATCAACAAGTCAAAGCAAAATAATTATTCCTAAAGCAGCTTACAAGCTAAGTGCAATTGAGCTGGTTGGCCCTTGCAAAAGTCCTATTGAAGTTCAACTTCAAGGAACATTGCAAGCACCACCAGATCTAACTGGAGAAGGATGGGTTAGTTTCAAATATATCGACTACTTTACATTGACAGGTGGTGGAGTTTTGGACGGGCAAGGGAAAGTAGCTTGGGAAAATAATGATTGTGGTAAAAATCCAAAGTGCGATAGAATTCCCATGGTAAGCACACTTTAATCATCTAATAAATATTTTTTTGTACTCCCATCCTATATATTAGTTAAAGTATTATTATTATTTTGTTGAAAATATGCAGAGTTTGAAGCTAAGCTTTATCACAAATTCGATAGTGAGTGATATAACATCTAAAGATAGCAAGAACTTTCATGTCAATGTATTGGGTTGTAAGAACCTCACCTTCCAGCATTTTACCATAGATGCACCAGGAAATAGTATTAATACTGATGGAATTCACATTGGTCGATCTGTTGGGATTAATGTCATCAATACGAACATTGGTACTGGAGATGATTGTATCTCACTTGGGGATGGAAGTAAGCAAGTAACAATTATTAATGTGACATGTGGACCTGGACATGGTATTAGTGTAGGAAGCCTTGGAAAGTACAATGTTGAAGAACCAGTAAAGGGAATCATAGTTAAGAACTGTACATTGATAAATACATCAAATGGTCTTAGAATCAAAACATGGCCTGCTTCTCCTGCTGCTGGAACTGCTTCTGATATGCATTATGAAGATATAGTAATGGTCAATGTCAGCAACCCAGTTCTCATAGATCAAGAGTATTGTCCATACAACCTATGTAATAAAAAGGTACAAAAGTTGTGGAACATTTTATTTTCTTTCATTGAATTTATAAATATTTAAACTAAAATGATGAAACTATATGTTCTATTTTCATGTTTTTCAGAGTCCATCAAAAGTCAAGATTAGTAAAGTTAGTTTCAAGAACATTAGAGGTATTTCAAGTAATGCGCTCGCAGTCAAACTCGTCTGTAGTTCTGAAGTTCCTTGTGAAGATGTAGAGCTAGCTGACATTGACCTGACGTACAGTGGAAGTGAAGGTCCAATCATATCTCAATGTTTGAATGTTAAGCCCACCATTTCTGGGAAGCAAAACCCTTCTATTTGTTCTTCTCGTACTCCTAATATTGCTCCATGA

mRNA sequence

ATGTGTACAACCTTCAACTTTGTGGCTCTATTATTTTTTATATTATTTTTTGTCTCCAGAGGCCAAACCCAATCCAACGACGGTGTTTTCGATGTGACAAAGTATGGTGCTAAGCCTAATGTTGATATATCTAAGGCTCTAACAAGTGCATGGAAGGAAGCTTGTGCATCAACAAGTCAAAGCAAAATAATTATTCCTAAAGCAGCTTACAAGCTAAGTGCAATTGAGCTGGTTGGCCCTTGCAAAAGTCCTATTGAAGTTCAACTTCAAGGAACATTGCAAGCACCACCAGATCTAACTGGAGAAGGATGGGTTAGTTTCAAATATATCGACTACTTTACATTGACAGGTGGTGGAGTTTTGGACGGGCAAGGGAAAGTAGCTTGGGAAAATAATGATTGTGGTAAAAATCCAAAGTGCGATAGAATTCCCATGAGTTTGAAGCTAAGCTTTATCACAAATTCGATAGTGAGTGATATAACATCTAAAGATAGCAAGAACTTTCATGTCAATGTATTGGGTTGTAAGAACCTCACCTTCCAGCATTTTACCATAGATGCACCAGGAAATAGTATTAATACTGATGGAATTCACATTGGTCGATCTGTTGGGATTAATGTCATCAATACGAACATTGGTACTGGAGATGATTGTATCTCACTTGGGGATGGAAGTAAGCAAGTAACAATTATTAATGTGACATGTGGACCTGGACATGGTATTAGTGTAGGAAGCCTTGGAAAGTACAATGTTGAAGAACCAGTAAAGGGAATCATAGTTAAGAACTGTACATTGATAAATACATCAAATGGTCTTAGAATCAAAACATGGCCTGCTTCTCCTGCTGCTGGAACTGCTTCTGATATGCATTATGAAGATATAGTAATGGTCAATGTCAGCAACCCAGTTCTCATAGATCAAGAGTATTGTCCATACAACCTATGTAATAAAAAGAGTCCATCAAAAGTCAAGATTAGTAAAGTTAGTTTCAAGAACATTAGAGGTATTTCAAGTAATGCGCTCGCAGTCAAACTCGTCTGTAGTTCTGAAGTTCCTTGTGAAGATGTAGAGCTAGCTGACATTGACCTGACGTACAGTGGAAGTGAAGGTCCAATCATATCTCAATGTTTGAATGTTAAGCCCACCATTTCTGGGAAGCAAAACCCTTCTATTTGTTCTTCTCGTACTCCTAATATTGCTCCATGA

Coding sequence (CDS)

ATGTGTACAACCTTCAACTTTGTGGCTCTATTATTTTTTATATTATTTTTTGTCTCCAGAGGCCAAACCCAATCCAACGACGGTGTTTTCGATGTGACAAAGTATGGTGCTAAGCCTAATGTTGATATATCTAAGGCTCTAACAAGTGCATGGAAGGAAGCTTGTGCATCAACAAGTCAAAGCAAAATAATTATTCCTAAAGCAGCTTACAAGCTAAGTGCAATTGAGCTGGTTGGCCCTTGCAAAAGTCCTATTGAAGTTCAACTTCAAGGAACATTGCAAGCACCACCAGATCTAACTGGAGAAGGATGGGTTAGTTTCAAATATATCGACTACTTTACATTGACAGGTGGTGGAGTTTTGGACGGGCAAGGGAAAGTAGCTTGGGAAAATAATGATTGTGGTAAAAATCCAAAGTGCGATAGAATTCCCATGAGTTTGAAGCTAAGCTTTATCACAAATTCGATAGTGAGTGATATAACATCTAAAGATAGCAAGAACTTTCATGTCAATGTATTGGGTTGTAAGAACCTCACCTTCCAGCATTTTACCATAGATGCACCAGGAAATAGTATTAATACTGATGGAATTCACATTGGTCGATCTGTTGGGATTAATGTCATCAATACGAACATTGGTACTGGAGATGATTGTATCTCACTTGGGGATGGAAGTAAGCAAGTAACAATTATTAATGTGACATGTGGACCTGGACATGGTATTAGTGTAGGAAGCCTTGGAAAGTACAATGTTGAAGAACCAGTAAAGGGAATCATAGTTAAGAACTGTACATTGATAAATACATCAAATGGTCTTAGAATCAAAACATGGCCTGCTTCTCCTGCTGCTGGAACTGCTTCTGATATGCATTATGAAGATATAGTAATGGTCAATGTCAGCAACCCAGTTCTCATAGATCAAGAGTATTGTCCATACAACCTATGTAATAAAAAGAGTCCATCAAAAGTCAAGATTAGTAAAGTTAGTTTCAAGAACATTAGAGGTATTTCAAGTAATGCGCTCGCAGTCAAACTCGTCTGTAGTTCTGAAGTTCCTTGTGAAGATGTAGAGCTAGCTGACATTGACCTGACGTACAGTGGAAGTGAAGGTCCAATCATATCTCAATGTTTGAATGTTAAGCCCACCATTTCTGGGAAGCAAAACCCTTCTATTTGTTCTTCTCGTACTCCTAATATTGCTCCATGA

Protein sequence

MCTTFNFVALLFFILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSSRTPNIAP
BLAST of Cla021897 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 1.8e-127
Identity = 224/363 (61.71%), Postives = 264/363 (72.73%), Query Frame = 1

Query: 42  DISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPD--- 101
           D ++ALT+AWKEACAS S S I++PK  + +  I L GPCKS I +QLQGTL+AP D   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 102 LTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKN-PKCDRIPMSLKLSFITNSIV 161
           + G GW++   ID  T+ GGGV DGQGK AW  NDC KN P C  + M+L+L  +TNSI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 162 SDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDD 221
            D+T+ DSKNFHVNV+GCKNLTF+ F I A   SINTDGIHIGRS G+N+INT I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 222 CISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTW 281
           CISLGDGSK + I N+TCGPGHGISVGSLG+Y  EE V GI VKNCT+  + NG+RIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 282 PASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGIS 341
           P S   G AS+MH++DI M +V  P+LIDQ YCPYN C  + PS VK+SK+SFKNI+G S
Sbjct: 241 PKS-EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 342 SNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSSRTP 401
           +   AVKLVCS   PC  VELADIDLTYSG  GP  S C N+KPTI GKQ P+ICS    
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAICSGSAA 360

BLAST of Cla021897 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.1e-124
Identity = 217/393 (55.22%), Postives = 287/393 (73.03%), Query Frame = 1

Query: 6   NFVALLFFILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIII 65
           +F  +L F+  F   G++Q+  GVFD+TKYGA  N DIS+AL +A+KEAC STS S I+I
Sbjct: 8   HFALVLLFLAHF---GESQT--GVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVI 67

Query: 66  PKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLT----GEGWVSFKYIDYFTLTGGGVL 125
           PK  + ++ ++L GPCKSP+E+Q+Q TL+AP D +    GE W++   +D FT++GGG+L
Sbjct: 68  PKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGE-WLTVNKLDQFTMSGGGIL 127

Query: 126 DGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQ 185
           DGQ   AWE   C ++ KC+++P +L  + +TNS + DIT+ DSK+FHVNV  CKNLTF 
Sbjct: 128 DGQAAAAWE---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFI 187

Query: 186 HFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGI 245
            F + AP NS NTDGIH+ RS  +N+ ++N  TGDDCIS+GD ++Q+ I  VTCGPGHGI
Sbjct: 188 RFNVSAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGI 247

Query: 246 SVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSN 305
           SVGSLG    E+PV G+ V+NCT  NT NG+RIKTWPAS   G  +D+H+EDI++ NVSN
Sbjct: 248 SVGSLGGNPDEKPVVGVFVRNCTFTNTDNGVRIKTWPAS-HPGVVNDVHFEDIIVQNVSN 307

Query: 306 PVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADI 365
           PV+IDQ YCP+N CNK  PS+VKISKVSF+NI+G S    AV L+ S  VPCE +E+ DI
Sbjct: 308 PVVIDQVYCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDI 367

Query: 366 DLTYSGSEGPIISQCLNVKPTISGKQNPSICSS 395
           D+TYSG EGP  S C N+KP++ GKQNP +C++
Sbjct: 368 DITYSGKEGPAKSSCENIKPSLKGKQNPPVCTA 390

BLAST of Cla021897 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.5e-124
Identity = 217/386 (56.22%), Postives = 279/386 (72.28%), Query Frame = 1

Query: 9   ALLFFILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKA 68
           A++   LF       QS  GV D++K+G KPN DI +ALTSAW EACAST+ +KI+IP  
Sbjct: 6   AIIVSFLFIADFCAAQS--GVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAG 65

Query: 69  AYKLSAIELVGPCKSPIEVQLQGTLQAPPDLT----GEGWVSFKYIDYFTLTGGGVLDGQ 128
            Y+L+ IEL GPCK+PIE+Q+ GT+QAP D +     E W  F Y+D+ TL+G GV DGQ
Sbjct: 66  TYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQ 125

Query: 129 G-----KVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLT 188
           G     K A  +   GKN    ++ M+   +F+ NSIV  +TSKDSKNFHV V GCKN+T
Sbjct: 126 GATVYKKAAPASAWSGKNSN-SKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNIT 185

Query: 189 FQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGH 248
           F  FTI APG+S NTDGIH+G+S  + ++NTNIGTGDDC+S+GDGSKQ+T+  V CGPGH
Sbjct: 186 FDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGH 245

Query: 249 GISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNV 308
           G+SVGSLGK+  EE V+GI VKNCTL  T NG+RIKTWP +P   T SD+H+EDI M NV
Sbjct: 246 GLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNV 305

Query: 309 SNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELA 368
            NPV+IDQEY P+N C+KK+PSK+K+SK+SFKN++G S  A  V L+CSS VPC+ VEL 
Sbjct: 306 KNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELN 365

Query: 369 DIDLTYSGSEGPIISQCLNVKPTISG 386
           ++DL ++G+  P  ++C NVKP ++G
Sbjct: 366 NVDLKFNGA--PTTAKCTNVKPLVTG 386

BLAST of Cla021897 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 5.1e-122
Identity = 212/404 (52.48%), Postives = 280/404 (69.31%), Query Frame = 1

Query: 1   MCTTFNFVALLF-FILFFVSRGQTQSNDGVFDVT-KYGAKPN--VDISKALTSAWKEACA 60
           M    N V  +F  +L F+S  + QS+   FDV  K+GAK +   D+SK    AWKEACA
Sbjct: 1   MAPHLNIVPSMFVLLLLFISASKVQSD--AFDVVAKFGAKADGKTDLSKPFLDAWKEACA 60

Query: 61  STSQSKIIIPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLTG---EGWVSFKYIDYF 120
           S + S ++IPK  Y LS + L GPCK+PIE+ +QGT+QAP D +      WV F  ++ F
Sbjct: 61  SVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENF 120

Query: 121 TLTGGGVLDGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVL 180
            + GGG+ DGQG +A+E N C       ++P++++  F+TN+++ DITSKDSK FH+NV 
Sbjct: 121 KMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVF 180

Query: 181 GCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINV 240
            CKN+T +   I+AP  S NTDGIH+G+S G+N+I ++I TGDDCIS+GDG+K + I  +
Sbjct: 181 ACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEI 240

Query: 241 TCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYED 300
           TCGPGHGIS+GSLGK+  EEPV+GI + NCT+ NTSNG RIKTWP     G  S++H+ED
Sbjct: 241 TCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFED 300

Query: 301 IVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPC 360
           I M NVS+P+LIDQ+YCP+N C K   SKVK+S +SFKNIRG S+   A+K +CS   PC
Sbjct: 301 ITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPC 360

Query: 361 EDVELADIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSSRTP 398
           ++VELADID+ ++G+E P  SQCLNVKP  SGK NP  CS   P
Sbjct: 361 QNVELADIDIKHNGAE-PATSQCLNVKPITSGKLNPIPCSGPVP 400

BLAST of Cla021897 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 7.4e-121
Identity = 210/404 (51.98%), Postives = 278/404 (68.81%), Query Frame = 1

Query: 1   MCTTFNFVALLF-FILFFVSRGQTQSNDGVFDVT-KYGAKPN--VDISKALTSAWKEACA 60
           M    N V  +F  +L F+S  + Q +   FDV  K+GAK +   D+SK    AWKEACA
Sbjct: 1   MAPHLNIVPSMFVLLLLFISASKVQPD--AFDVVAKFGAKADGKTDLSKPFLDAWKEACA 60

Query: 61  STSQSKIIIPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLTG---EGWVSFKYIDYF 120
           S + S ++IPK  Y LS + L GPCK+PIE+ +QGT+QAP D +      WV F  ++ F
Sbjct: 61  SVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENF 120

Query: 121 TLTGGGVLDGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVL 180
            + GGG+ DGQG +A+E N C       ++P++++  F+TN+++ DITSKDSK FH+NV 
Sbjct: 121 KMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVF 180

Query: 181 GCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINV 240
            CKN+T +   I+AP  S NTDGIH+G+S G+N+I ++I TGDDCIS+GDG+K + I  +
Sbjct: 181 ACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEI 240

Query: 241 TCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYED 300
           TCGPGHGIS+GSLGK+  EEPV+GI + NCT+ NTSNG RIKTWP     G  S++H+ED
Sbjct: 241 TCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGE-HGGAVSEIHFED 300

Query: 301 IVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPC 360
           I M NVS+P+LIDQ+YCP+N C K   SKVK+S +SFKNIRG S+   A+K +CS   PC
Sbjct: 301 ITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPC 360

Query: 361 EDVELADIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSSRTP 398
           ++VELADID+ ++G+E P  SQCLNVKP   GK NP  CS   P
Sbjct: 361 QNVELADIDIQHNGAE-PATSQCLNVKPITIGKLNPIPCSGPVP 400

BLAST of Cla021897 vs. TrEMBL
Match: E3VSV7_CUCPE (Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.3e-140
Identity = 240/392 (61.22%), Postives = 296/392 (75.51%), Query Frame = 1

Query: 7   FVALLFFILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIP 66
           F+AL+  +L  VS     +  GVFD+T +GAKPN D ++AL  AWKEACAS + SK+++P
Sbjct: 17  FMALIPLLLLLVSNATVGATSGVFDITTFGAKPNADATQALADAWKEACASATPSKVLVP 76

Query: 67  KAAYKLSAIELVGPCKSPIEVQLQGTLQAPP-DLTGEGWVSFKYIDYFTLTGGGVLDGQG 126
           K  Y+L    L GPCKSPIE+ ++GTLQA P D  G+G +  +YID+ TL+G GV DGQG
Sbjct: 77  KGTYQLGESRLKGPCKSPIELLVEGTLQASPTDTEGDGLLVMEYIDHLTLSGTGVFDGQG 136

Query: 127 KVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTI 186
           K  WE NDC    +C ++PM+LK +FITNSIV DITS DSKNFH+NVL  KNLTF H  I
Sbjct: 137 KAGWEKNDCHLKKECKKLPMNLKFNFITNSIVKDITSLDSKNFHINVLAGKNLTFDHLKI 196

Query: 187 DAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGS 246
            AP NS NTDGIH+G S  + ++NT I TGDDCIS+G  ++++TI +VTCGPGHGIS+GS
Sbjct: 197 TAPHNSPNTDGIHLGDSEDVYILNTAIATGDDCISVGYTNRKITISDVTCGPGHGISIGS 256

Query: 247 LGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLI 306
           LGKY  E+ V G+ VK C LI T+NG+RIK+WP S  +  ASDMH+EDI M NV+NP++I
Sbjct: 257 LGKYKTEKEVVGVTVKKCKLIGTTNGVRIKSWPDSAVSYPASDMHFEDIEMENVANPIII 316

Query: 307 DQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTY 366
           DQEYCP+N CN+K PS +KISKVSFKNIRG SS  +AVKLVCS   PCE VE+ADIDLTY
Sbjct: 317 DQEYCPWNQCNRKVPSAIKISKVSFKNIRGTSSTPVAVKLVCSKSNPCEGVEIADIDLTY 376

Query: 367 SGSEGPIISQCLNVKPTISGKQNPSICSSRTP 398
           SG EGP +S+C NVKPTISGKQNP IC+   P
Sbjct: 377 SGKEGPAVSECSNVKPTISGKQNPPICAKPAP 408

BLAST of Cla021897 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.2e-138
Identity = 237/395 (60.00%), Postives = 298/395 (75.44%), Query Frame = 1

Query: 1   MCTTFNFVALLFFILFFVSRGQTQSNDGVFDVTKYG-AKPNVDISKALTSAWKEACASTS 60
           + TTF    L F I       + +S+  +F + KYG      D+++AL  AW +ACAS  
Sbjct: 10  LVTTFLMSLLSFTI-------EARSHVDIFSLRKYGNIVSGADVTEALKDAWNDACASDR 69

Query: 61  QSKIIIPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLTGEGWVSFKYIDYFTLTGGG 120
            S ++IPK  +K+   E  GPCKSPIE ++ GTLQAP    G+ W++F Y+D   L+GGG
Sbjct: 70  PSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGG 129

Query: 121 VLDGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLT 180
           V DGQGK  WE NDC K   C ++P+SL+  FITNSIV  ITS DSKNFHVN+LGC NLT
Sbjct: 130 VFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCNNLT 189

Query: 181 FQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGH 240
           FQ   I AP NS NTDGIHIGRS+GI+++ + I TGDDCISLGDGSK++ + NV CGPGH
Sbjct: 190 FQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICGPGH 249

Query: 241 GISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNV 300
           GIS+GSLGKYN EEPV+G+IV NCT+INT+NG+RIKTWPASP AG A++MH+ DI MVNV
Sbjct: 250 GISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVNV 309

Query: 301 SNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELA 360
           SNP+LIDQEYCP+N CN++ PS +KI+KVSFKNIRG S+  +AVKL+CSS +PCE+V++A
Sbjct: 310 SNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKVA 369

Query: 361 DIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSS 395
           +IDL Y+G +G I S+C+NVKP ISG QNP ICSS
Sbjct: 370 NIDLVYNGIKGSITSECMNVKPIISGIQNPPICSS 397

BLAST of Cla021897 vs. TrEMBL
Match: Q9MBC0_SALGI (Polygalacturonase OS=Salix gilgiana GN=SgPG1 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.0e-136
Identity = 240/388 (61.86%), Postives = 294/388 (75.77%), Query Frame = 1

Query: 8   VALLFFILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPK 67
           + L   +L   S  + QSN GVFDVTKYG K   DI+ ALT+AWK+ACAST  SK+ IP 
Sbjct: 10  IVLFSLLLLLASTAKAQSN-GVFDVTKYGGKQ--DITAALTNAWKDACASTKPSKVRIPS 69

Query: 68  AAYKLSAIELVGPCKSPIEVQLQGTLQAP--PD-LTGEGWVSFKYIDYFTLTGGGVLDGQ 127
             Y L  + L GPCKS IE+Q+ G L+AP  PD  +G  WV+F+YID+ TL+G G  DGQ
Sbjct: 70  GTYSLRQVTLAGPCKSAIELQVNGILKAPVNPDQFSGGHWVNFRYIDHLTLSGSGTFDGQ 129

Query: 128 GKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFT 187
           G VAW  + C KN  C+ +PM+++  FITN +V DIT++DSKNFHVNVLGCKNLTFQHFT
Sbjct: 130 GNVAWSKSTCSKNKNCEGLPMNIRFDFITNGLVRDITTRDSKNFHVNVLGCKNLTFQHFT 189

Query: 188 IDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVG 247
           + AP  SINTDGIHIGRS GI +I++ IGTGDDCIS+GDG++++ +  VTCGPGHGISVG
Sbjct: 190 VTAPAESINTDGIHIGRSTGIYIIDSKIGTGDDCISVGDGTEELHVTRVTCGPGHGISVG 249

Query: 248 SLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVL 307
           SLG+Y  E+PV GI VKNCT+ NT+NG+RIK+WP     G AS+MH+EDIVM NV NP+L
Sbjct: 250 SLGRYPNEKPVSGIFVKNCTISNTANGVRIKSWP-DLYGGVASNMHFEDIVMNNVQNPIL 309

Query: 308 IDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLT 367
           +DQ YCP+N C+ K+PSKVKIS VSFKNIRG S+  + VKL CSS +PCE VELA+I+L 
Sbjct: 310 LDQVYCPWNQCSLKAPSKVKISDVSFKNIRGTSATPVVVKLACSSGIPCEKVELANINLV 369

Query: 368 YSGSEGPIISQCLNVKPTISGKQNPSIC 393
           YSGSEGP  SQC NVKP ISG  + S C
Sbjct: 370 YSGSEGPAKSQCSNVKPKISGIMSASGC 393

BLAST of Cla021897 vs. TrEMBL
Match: A0A061DTU7_THECC (Pectin lyase-like superfamily protein, putative OS=Theobroma cacao GN=TCM_002294 PE=3 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.6e-136
Identity = 237/393 (60.31%), Postives = 294/393 (74.81%), Query Frame = 1

Query: 8   VALLFFILFFVSRGQTQSNDGVFDVT-KYGAKPN--VDISKALTSAWKEACASTSQSKII 67
           +A+L  +L   S    ++   V DV  KYGAK +   D+S  L  AWKEACAST+ SKI+
Sbjct: 7   IAVLSILLLLFSTSIPKAQSTVVDVVAKYGAKADEKTDLSTPLLHAWKEACASTTPSKIV 66

Query: 68  IPKAAYKLSAIELVGPCKSPIEVQLQGTLQAPPDLTG---EGWVSFKYIDYFTLTGGGVL 127
           IPK  Y+LS   L GPCKS IE+Q+ GT++AP D +      WV F ++D+ T++GGGV 
Sbjct: 67  IPKGTYRLSRATLDGPCKSAIELQVSGTIKAPADPSAFKDPNWVVFNHVDHLTISGGGVF 126

Query: 128 DGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQ 187
           DGQG   W  N+C KN  C  +P++L+ +F++N+IV  IT+KDSK FHVNVLGCKN TFQ
Sbjct: 127 DGQGAAVWGKNNCAKNKYCAALPINLRFNFVSNAIVQGITTKDSKQFHVNVLGCKNFTFQ 186

Query: 188 HFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGI 247
           HFT+ AP NSINTDGIHIGRS GI +++TNI TGDDC+SLGDGSK V +  VTCGPGHGI
Sbjct: 187 HFTVSAPENSINTDGIHIGRSNGIKILDTNIKTGDDCVSLGDGSKNVIVERVTCGPGHGI 246

Query: 248 SVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSN 307
           SVGSLGK+  EEPV GI ++NCT+ NT NG+R+KTWP S   G+A+DMH+EDI++ NV N
Sbjct: 247 SVGSLGKFKGEEPVSGIFIRNCTISNTMNGVRVKTWPDS-HPGSATDMHFEDIILNNVGN 306

Query: 308 PVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADI 367
           P+LIDQEYCP+NLCN K PS+VK+S +SFK I G SS   AVKLVCS  +PC+ VELADI
Sbjct: 307 PILIDQEYCPWNLCNLKVPSRVKLSNISFKKIWGTSSTQQAVKLVCSRGLPCDRVELADI 366

Query: 368 DLTYSGSEGPIISQCLNVKPTISGKQNPSICSS 395
           D+TY G  GP ISQC+NVKP +SGKQNP  CSS
Sbjct: 367 DITYKGPGGPAISQCINVKPRLSGKQNPPACSS 398

BLAST of Cla021897 vs. TrEMBL
Match: Q9MBB9_SALGI (Polygalacturonase OS=Salix gilgiana GN=SgPG2 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.3e-135
Identity = 237/382 (62.04%), Postives = 293/382 (76.70%), Query Frame = 1

Query: 14  ILFFVSRGQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLS 73
           +L   S  + QSN GVFDVTKYG K   DI++AL +AWK+ACAST+ SK++IP   Y L 
Sbjct: 16  LLLLASTAKAQSN-GVFDVTKYGGKE--DITEALNNAWKDACASTNPSKVLIPSGTYSLR 75

Query: 74  AIELVGPCKSPIEVQLQGTLQAP--PD-LTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWE 133
            + L GPCK+ IE+Q+ G L+AP  PD  +G  WV+F+YID  TL+G G  DGQG VAW 
Sbjct: 76  QVTLAGPCKAAIELQVNGILKAPVNPDQFSGSHWVNFRYIDQLTLSGSGTFDGQGNVAWS 135

Query: 134 NNDCGKNPKCDRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGN 193
            + C KN  C+ +PM+++  FITN +V DIT++DSKNFHVNVLGCKNLTFQHFT+ AP  
Sbjct: 136 KSTCSKNKNCEGLPMNIRFDFITNGLVRDITTRDSKNFHVNVLGCKNLTFQHFTVTAPAE 195

Query: 194 SINTDGIHIGRSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYN 253
           SINTDGIHIGRS GI +I++ IGTGDDCIS+GDG++++ +  VTCGPGHGISVGSLG+Y 
Sbjct: 196 SINTDGIHIGRSTGIYIIDSKIGTGDDCISVGDGTEELHVTGVTCGPGHGISVGSLGRYP 255

Query: 254 VEEPVKGIIVKNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYC 313
            E+PV GI VKNCT+ NT+NG+RIK+WP     G AS+MH+EDIVM NV NP+L+DQ YC
Sbjct: 256 NEKPVSGIFVKNCTISNTANGVRIKSWP-DLYGGVASNMHFEDIVMNNVQNPILLDQVYC 315

Query: 314 PYNLCNKKSPSKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEG 373
           P+N C+ K+PSKVKIS VSFKNIRG S+  + VKL CSS +PCE VELA+I+L YSGSEG
Sbjct: 316 PWNQCSLKAPSKVKISDVSFKNIRGTSATPVVVKLACSSGIPCEKVELANINLLYSGSEG 375

Query: 374 PIISQCLNVKPTISGKQNPSIC 393
           P  SQC NVKP ISG  + S C
Sbjct: 376 PAKSQCSNVKPKISGIMSASGC 393

BLAST of Cla021897 vs. NCBI nr
Match: gi|659119537|ref|XP_008459709.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 613.2 bits (1580), Expect = 3.2e-172
Identity = 289/366 (78.96%), Postives = 318/366 (86.89%), Query Frame = 1

Query: 29  VFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGPCKSPIEVQ 88
           +FDVTKYGAKPN DI++AL  AWKEACASTS  KI+IP   YKL  IEL G CKSPIE+Q
Sbjct: 54  IFDVTKYGAKPNADITQALADAWKEACASTSPCKIVIPTGTYKLGTIELKGECKSPIEIQ 113

Query: 89  LQGTLQAPPDLTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKCDRIPMSLK 148
           +QGTLQAPPDLTGE WV FKYIDY ++T GGV DGQGK AWE+NDC KNPKC RIPM+LK
Sbjct: 114 VQGTLQAPPDLTGEDWVHFKYIDYLSVTSGGVFDGQGKQAWESNDCDKNPKCARIPMNLK 173

Query: 149 LSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVI 208
            SFI N+IVSDITSKDSKNFH+ VLGC NLTFQH  ++AP  SINTDGIHIGRS+GIN++
Sbjct: 174 FSFIKNAIVSDITSKDSKNFHIQVLGCNNLTFQHVNVNAPETSINTDGIHIGRSLGINIL 233

Query: 209 NTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINT 268
           NTNIGTGDDCISLGDGSKQVTI NVTCGPGHGISVGSLG+YN+EEPV+G+ VKNCTL  T
Sbjct: 234 NTNIGTGDDCISLGDGSKQVTITNVTCGPGHGISVGSLGRYNLEEPVEGLQVKNCTLTGT 293

Query: 269 SNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKV 328
           +NGLRIKTWPASPAAG ASDMHYED+VM NV NP+LIDQEYCPYN CNK+ PSKVKISKV
Sbjct: 294 TNGLRIKTWPASPAAGVASDMHYEDVVMNNVMNPILIDQEYCPYNQCNKQVPSKVKISKV 353

Query: 329 SFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVKPTISGKQN 388
           SFKNIRG S NA AV LVCSS  PC+ VELADIDLTYSGSEGPI S+C NVKPT+SGKQN
Sbjct: 354 SFKNIRGTSGNAAAVTLVCSSSNPCDGVELADIDLTYSGSEGPITSRCANVKPTVSGKQN 413

Query: 389 PSICSS 395
           P  C+S
Sbjct: 414 PPACTS 419

BLAST of Cla021897 vs. NCBI nr
Match: gi|778708536|ref|XP_011656227.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 562.8 bits (1449), Expect = 4.9e-157
Identity = 264/378 (69.84%), Postives = 315/378 (83.33%), Query Frame = 1

Query: 21  GQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGP 80
           G+ QSNDG FDVTK+GA PN +I +ALT AWK AC STSQS+++IP+ AYKLS I+L GP
Sbjct: 2   GKGQSNDGDFDVTKFGAMPNTEIFQALTDAWKAACGSTSQSQVLIPQGAYKLSKIQLTGP 61

Query: 81  CKSPIEVQLQGTLQAPPDLTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKC 140
           CKS I++QLQG LQAP DL G+ W+SF+ ID FTLTG GV DGQGK+AW  NDC KNP C
Sbjct: 62  CKSHIQIQLQGILQAPVDLVGDNWISFQNIDQFTLTGQGVFDGQGKIAWGKNDCNKNPNC 121

Query: 141 DRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIG 200
            ++P++++  +I NSIVSDITSKDSK+FH NVLGCKNLTF+H  I+AP +S NTDGIHIG
Sbjct: 122 AKLPINVRFDYIKNSIVSDITSKDSKSFHFNVLGCKNLTFKHVNINAPQDSPNTDGIHIG 181

Query: 201 RSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIV 260
           RSVGINVINT IGTGDDCISLGDGS+QVTI NVTC PGHGISVGSLG+Y  E PV+G+  
Sbjct: 182 RSVGINVINTKIGTGDDCISLGDGSQQVTITNVTCAPGHGISVGSLGRYVGETPVQGVRA 241

Query: 261 KNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSP 320
           KNCTLINT+NG+RIKT P+SP+ G ASD+H+EDI+MVNV NP++IDQEYCP+N C+KK+P
Sbjct: 242 KNCTLINTTNGVRIKTRPSSPSFGVASDIHFEDIIMVNVINPLVIDQEYCPWNQCDKKNP 301

Query: 321 SKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVK 380
           SKVKIS VSF NIRG ++N LAV  VCSS+ PCED+E+ DI+LTY+GS GPI S C NVK
Sbjct: 302 SKVKISNVSFINIRGTAANTLAVSFVCSSQFPCEDIEVTDINLTYNGSGGPISSMCKNVK 361

Query: 381 PTISGKQNPSICSSRTPN 399
           P +SG QNP IC SR P+
Sbjct: 362 PKVSGVQNPHICPSRAPS 379

BLAST of Cla021897 vs. NCBI nr
Match: gi|778730770|ref|XP_011659855.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 555.8 bits (1431), Expect = 6.0e-155
Identity = 262/383 (68.41%), Postives = 316/383 (82.51%), Query Frame = 1

Query: 21  GQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGP 80
           G+ QSNDG FDVTK+GA PN +I +ALT AWK AC S SQS+++IP+ AYKLS I+L GP
Sbjct: 2   GKGQSNDGDFDVTKFGAMPNTEIFQALTDAWKAACGSASQSQVLIPQGAYKLSKIQLTGP 61

Query: 81  CKSPIEVQLQGTLQAPPDLTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKC 140
           CKS I++QLQG LQAP DL G+ W+SF+ ID FTLTG GV DGQGK+AW  NDC KNP C
Sbjct: 62  CKSHIQIQLQGILQAPVDLVGDNWISFQNIDQFTLTGKGVFDGQGKIAWGKNDCNKNPNC 121

Query: 141 DRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIG 200
            ++P++++  +I NSIVSDITSKDSK+FH NVLGCKNLTF+H  I+AP +S NTDGIHIG
Sbjct: 122 AKLPINVRFDYIKNSIVSDITSKDSKSFHFNVLGCKNLTFKHVNINAPQDSPNTDGIHIG 181

Query: 201 RSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIV 260
           RS+GINVINT I TGDDCISLGDGS+QVTI NVTC PGHGISVGSLG+Y  E PV+G+  
Sbjct: 182 RSIGINVINTKISTGDDCISLGDGSQQVTITNVTCAPGHGISVGSLGRYVGETPVQGVRA 241

Query: 261 KNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSP 320
           KNCTLINT+NG+RIKT P+SP+ G ASD+H+EDI+MVNV NP++IDQEYCP+N C+KK+P
Sbjct: 242 KNCTLINTTNGVRIKTRPSSPSFGVASDIHFEDIIMVNVINPLVIDQEYCPWNQCDKKNP 301

Query: 321 SKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVK 380
           SKVKIS VSF NIRG ++N LAV  VCSS+ PCED+E+ DI+LTY+GS GPI S C NVK
Sbjct: 302 SKVKISNVSFINIRGTAANTLAVSFVCSSQFPCEDIEVTDINLTYNGSGGPIASMCKNVK 361

Query: 381 PTISGKQNPSIC--SSRTPNIAP 402
           P +SG QNP IC  S+ +P+ AP
Sbjct: 362 PKLSGVQNPHICPSSALSPSFAP 384

BLAST of Cla021897 vs. NCBI nr
Match: gi|659131743|ref|XP_008465833.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 550.1 bits (1416), Expect = 3.3e-153
Identity = 262/374 (70.05%), Postives = 309/374 (82.62%), Query Frame = 1

Query: 21  GQTQSNDGVFDVTKYGAKPNVDISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGP 80
           G+ QSND  FDVTK+GA PN DI +ALT+AWK AC STSQS+++IP+  YKLS IEL GP
Sbjct: 2   GKGQSNDADFDVTKFGAMPNTDIFQALTNAWKAACGSTSQSQVLIPEETYKLSKIELTGP 61

Query: 81  CKSPIEVQLQGTLQAPPDLTGEGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKC 140
           CK+ I++QLQG LQAP DL  + W+SFKYID FTLTG GV DGQGKVAW  NDC  NPKC
Sbjct: 62  CKNRIQIQLQGILQAPADLAEDNWISFKYIDQFTLTGKGVFDGQGKVAWGKNDCAINPKC 121

Query: 141 DRIPMSLKLSFITNSIVSDITSKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIG 200
            +  ++++  FI N+IVSDITSKDSK+FH NVLGCKNLTFQ+  I+AP +S NTDGIHIG
Sbjct: 122 AKFAINVRFDFIKNAIVSDITSKDSKSFHFNVLGCKNLTFQNVNINAPEDSPNTDGIHIG 181

Query: 201 RSVGINVINTNIGTGDDCISLGDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIV 260
           RS+GINVINTNIGTGDDCISLGDGS+QV I NVTCGPGHGISVGSLG+Y  E PV+GI  
Sbjct: 182 RSIGINVINTNIGTGDDCISLGDGSQQVAITNVTCGPGHGISVGSLGRYVGEAPVQGIRA 241

Query: 261 KNCTLINTSNGLRIKTWPASPAAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSP 320
           KNCTLINT+NG+RIKT P+SP+ G ASD+H+EDI+MVNV NP++IDQEYCP N C+KK+P
Sbjct: 242 KNCTLINTTNGVRIKTRPSSPSLGVASDIHFEDIIMVNVINPMVIDQEYCPSNQCDKKNP 301

Query: 321 SKVKISKVSFKNIRGISSNALAVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVK 380
           SKVKIS VSF NIRG ++NALAV  VCSS+ PCED+++ DI+LTY+GS  PI S C  VK
Sbjct: 302 SKVKISNVSFTNIRGTAANALAVSFVCSSQFPCEDIKVTDINLTYNGSGDPISSTCKYVK 361

Query: 381 PTISGKQNPSICSS 395
           P +SG QNP ICSS
Sbjct: 362 PIVSGIQNPQICSS 375

BLAST of Cla021897 vs. NCBI nr
Match: gi|778708790|ref|XP_011656283.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 548.1 bits (1411), Expect = 1.3e-152
Identity = 261/356 (73.31%), Postives = 295/356 (82.87%), Query Frame = 1

Query: 43  ISKALTSAWKEACASTSQSKIIIPKAAYKLSAIELVGPCKS-PIEVQLQGTLQAPPDLTG 102
           + +AL +AWKEACAS   SK+IIP   Y L AIEL G C S PIE+Q+Q TL APPDL G
Sbjct: 1   MDRALATAWKEACASAGPSKVIIPSGTYTLGAIELKGECTSTPIEMQVQATLLAPPDLKG 60

Query: 103 EGWVSFKYIDYFTLTGGGVLDGQGKVAWENNDCGKNPKCDRIPMSLKLSFITNSIVSDIT 162
           E WV FKY++  +++GGGV DGQGK  WE+NDC KNPKC  +PMSLK SFI NS+VS IT
Sbjct: 61  EDWVHFKYVNDLSVSGGGVFDGQGKKTWESNDCHKNPKCAPMPMSLKFSFIKNSVVSGIT 120

Query: 163 SKDSKNFHVNVLGCKNLTFQHFTIDAPGNSINTDGIHIGRSVGINVINTNIGTGDDCISL 222
           SKDSKNFH+ VLGC N T Q+  ++APG SINTDGIH+GR VGIN++N+NIGTGDDCISL
Sbjct: 121 SKDSKNFHIQVLGCNNFTLQNVNVNAPGTSINTDGIHLGRCVGINILNSNIGTGDDCISL 180

Query: 223 GDGSKQVTIINVTCGPGHGISVGSLGKYNVEEPVKGIIVKNCTLINTSNGLRIKTWPASP 282
           GDGSKQVTI NVTCGPGHGISVGSLG+YN EEPV+G+ VKNCT+  T++GLRIKTWPASP
Sbjct: 181 GDGSKQVTITNVTCGPGHGISVGSLGRYNAEEPVEGLQVKNCTITGTTSGLRIKTWPASP 240

Query: 283 AAGTASDMHYEDIVMVNVSNPVLIDQEYCPYNLCNKKSPSKVKISKVSFKNIRGISSNAL 342
           AAG ASDMHYEDIVM NVSNP++IDQEYCPYN CNK+ PSKVKISKVSFKNIRG S NA 
Sbjct: 241 AAGVASDMHYEDIVMNNVSNPIMIDQEYCPYNKCNKQIPSKVKISKVSFKNIRGTSGNAQ 300

Query: 343 AVKLVCSSEVPCEDVELADIDLTYSGSEGPIISQCLNVKPTISGKQNPSICSSRTP 398
           AV LVCSS  PCE VELADIDLTY+G+EGPI S C NVKPTI+GKQNP ICS   P
Sbjct: 301 AVTLVCSSSNPCEGVELADIDLTYTGNEGPITSVCANVKPTITGKQNPPICSPLAP 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_OENOR1.8e-12761.71Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1[more]
PGLR_TOBAC1.1e-12455.22Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1[more]
PGLR_MEDSA5.5e-12456.22Polygalacturonase OS=Medicago sativa PE=2 SV=1[more]
PGLR_GOSHI5.1e-12252.48Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1[more]
PGLR_GOSBA7.4e-12151.98Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
E3VSV7_CUCPE1.3e-14061.22Polygalacturonase OS=Cucurbita pepo GN=PG1 PE=2 SV=1[more]
A0A0A0K3B7_CUCSA1.2e-13860.00Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1[more]
Q9MBC0_SALGI2.0e-13661.86Polygalacturonase OS=Salix gilgiana GN=SgPG1 PE=2 SV=1[more]
A0A061DTU7_THECC2.6e-13660.31Pectin lyase-like superfamily protein, putative OS=Theobroma cacao GN=TCM_002294... [more]
Q9MBB9_SALGI1.3e-13562.04Polygalacturonase OS=Salix gilgiana GN=SgPG2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
gi|659119537|ref|XP_008459709.1|3.2e-17278.96PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|778708536|ref|XP_011656227.1|4.9e-15769.84PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|778730770|ref|XP_011659855.1|6.0e-15568.41PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|659131743|ref|XP_008465833.1|3.3e-15370.05PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|778708790|ref|XP_011656283.1|1.3e-15273.31PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR006626PbH1
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla021897Cla021897.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 55..382
score: 5.3E
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 232..245
scor
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 225..245
score: 3800.0coord: 175..201
score: 36.0coord: 255..276
score:
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 16..394
score: 3.46E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 12..398
score: 1.6E
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 7..394
score: 3.6E
NoneNo IPR availablePANTHERPTHR31375:SF37PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 7..394
score: 3.6E