Cla019869 (gene) Watermelon (97103) v1

NameCla019869
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionKunitz trypsin inhibitor 4 (AHRD V1 **** B4UWB2_ARAHY); contains Interpro domain(s) IPR011065 Kunitz inhibitor ST1-like
LocationChr2 : 26342548 .. 26343084 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

mRNA sequence

ATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Coding sequence (CDS)

ATGGCCATAACTTCGATAGCTCAATTTCCTCCAGTTTTCGACACCGATGGTCAACCTCTGCGGCGGGGTGTCGAGTACTACATCTCGCCTGTATTTACCGATGTTGGGGGCAACCTCACCCTAAAAAGTCGTAGTAACGCTCCATGCCCGCTCTTCGTCGGCCAGGAACCGGTTACTTCAACGAATATCGGTCTCCCGGTCACCTTCACTCCCATCATGGCAGGCAAAGATATAATTGACGAAGGTACCAGTTTGAACATTGTGTTTCAAGCGTTGTCAACGTGCATAACATCGACGCAATGGAGAGTGGATGCGGCGGAGTCTGACACCGGGAGGAGGTTCGTGGGGATCGGGAGGGAGGACGGTCCTGCCGGGATCTTTGGGATCAGTAGGGACAATGGAGTTTACAATATAGTATGGTGTCCGGCGATGATGGGGAGGCCAAGGTGTGGAAGGGCTGGGATTTTGGTTGAGAATGGAGTGAGGCTGTTGGCTTTGGATGGTGAAGCGTTTCCTTTTGAGTTTGTGAAAGCTTGA

Protein sequence

MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGREDGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA
BLAST of Cla019869 vs. Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 95.1 bits (235), Expect = 8.1e-19
Identity = 69/190 (36.32%), Postives = 91/190 (47.89%), Query Frame = 1

Query: 5   SIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIG 64
           + +   PV D DG+ LR G  YYI PV  D GG LT+   +  P   FV    V  T   
Sbjct: 29  ADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVS--ATTPNGTFVCPPRVVQTRKE 88

Query: 65  L----PVTFTPIMAGKDIIDEGTSLNIVFQALSTC--ITSTQWRVDAAESDTGRRFVGIG 124
           +    P+ F P    +D++   T LNI F A   C   +ST WR+D  +  TG+ FV IG
Sbjct: 89  VDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTIG 148

Query: 125 REDGPAGIFGISR--------DNGVYNIVWCPAMMG--RPRCGRAGILV-ENGVRLLALD 178
              G  G   IS          +G Y +V+CP + G  + +CG  GI + + G R LAL 
Sbjct: 149 GVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKCGDVGIYIDQKGRRRLALS 208

BLAST of Cla019869 vs. Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 1.0e-13
Identity = 47/120 (39.17%), Postives = 61/120 (50.83%), Query Frame = 1

Query: 10  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 69
           PPV DTDG  LR    YY+       GG LT+       CPLFV Q+P    + G PV  
Sbjct: 26  PPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHDGFPVRI 85

Query: 70  TP--IMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG--REDGPAG 126
           TP  +     II   T + I F+A +TC+ ST+W +D +E   GRR V  G  ++  P+G
Sbjct: 86  TPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHID-SELAAGRRHVITGPVKDPSPSG 143

BLAST of Cla019869 vs. Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.3e-13
Identity = 49/120 (40.83%), Postives = 59/120 (49.17%), Query Frame = 1

Query: 10  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 69
           PPV DTDG  LR    YY+ P     GG LT+       CPLFV QE       GLPV  
Sbjct: 3   PPVHDTDGNELRADANYYVLPANRAHGGGLTMAPGHGRRCPLFVSQE-ADGQRDGLPVRI 62

Query: 70  TPI--MAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG--REDGPAG 126
            P        II   T + I F+A +TC+ ST+W +D +E  +GRR V  G  R+  P+G
Sbjct: 63  APHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHID-SELVSGRRHVITGPVRDPSPSG 120

BLAST of Cla019869 vs. Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 7.3e-12
Identity = 53/141 (37.59%), Postives = 65/141 (46.10%), Query Frame = 1

Query: 10  PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 69
           PPV+DT+G  L     YY+ P     GG LT+  R   PCPL V QE       G PV F
Sbjct: 25  PPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPRV-LPCPLLVAQE-TDERRKGFPVRF 84

Query: 70  TP---IMAGKD-IIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGREDGPA- 129
           TP     A +D  I   T + I F A + C+ ST+W V   E  TG R V  G   GP+ 
Sbjct: 85  TPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHV-GDEPLTGARRVVTGPLIGPSP 144

Query: 130 ----GIFGISRDNGVYNIVWC 142
                 F + +  G Y +V C
Sbjct: 145 SGRENAFRVEKYGGGYKLVSC 162

BLAST of Cla019869 vs. Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 6.9e-10
Identity = 49/151 (32.45%), Postives = 68/151 (45.03%), Query Frame = 1

Query: 11  PVFDTDGQPLRRGVEYYI-SPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTSTNIGLPVTF 70
           PV DTDG  L+ GV+YY+ S +    GG L L   +   CP  V Q      N G PV F
Sbjct: 30  PVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDN-GTPVIF 89

Query: 71  TPIMAGKDIIDEGTSLNIVFQAL--STCITSTQWRVDAAESDTGRRFV---GIGREDGP- 130
           +   +  D++   T +NI F  +    C TST WR+D  ++  G+ +V   G+  E GP 
Sbjct: 90  SNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKGEPGPN 149

Query: 131 ----------AGIFGISRDNGVYNIVWCPAM 145
                     AG+ G       Y   +CP++
Sbjct: 150 TLCSWFKIEKAGVLG-------YKFRFCPSV 172

BLAST of Cla019869 vs. TrEMBL
Match: A0A0A0LIK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 1.7e-87
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 1

Query: 1   MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 60
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 61  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 120
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 121 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 179
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

BLAST of Cla019869 vs. TrEMBL
Match: A0A0A0LGB4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-49
Identity = 104/183 (56.83%), Postives = 123/183 (67.21%), Query Frame = 1

Query: 1   MAITSIAQF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPV 60
           MAI S AQF  PPV DT+G+PL+R VEY+I P  TDV GNLTL +R    CP +VGQ P+
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  TSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG 120
            S  IG  VT TP + G D I EG  L  VFQA + CIT TQW+V   +  +GRRFV +G
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 REDGPAGIFGISRDN-GVYNIVWCPA---MMGRPRCGRAGILVENGVRLLALDGEAFPFE 178
            ++   G F I + N GVYNI WCP+     GRPRCG AGIL+E GVR LALDG AFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

BLAST of Cla019869 vs. TrEMBL
Match: A0A0X7YK90_HEVBR (Alpha-amylase/subtilisin inhibitor OS=Hevea brasiliensis GN=ASI PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 8.1e-42
Identity = 96/186 (51.61%), Postives = 121/186 (65.05%), Query Frame = 1

Query: 1   MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPV-T 60
           MA++++AQ P V DT+GQPLR GVEYY+ P  TD  G LTL +R+++ CPL+VGQEP+ T
Sbjct: 15  MAMSTVAQTPAVLDTNGQPLRSGVEYYVLPAATDTAGGLTLVNRTDS-CPLYVGQEPLST 74

Query: 61  STNIGLPVTFTPIMAGKD-IIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG 120
             + GL VTFTP  A  D II E    ++ F A+S C  ST WRV   +++T RRF+  G
Sbjct: 75  VVSQGLRVTFTPFAAQNDGIIRESREFSVAFSAVSICGQSTAWRVGEEDAETSRRFIVTG 134

Query: 121 REDGPAGIFGISRDNGVYNIVWCPA------MMGRPRCGRAGILVENGVRLLALDGEAFP 179
              G    F I  + G+Y +VWCP         GRPRCG AGIL+ENG RLLALDG AFP
Sbjct: 135 ---GEQSYFRIDNNGGLYELVWCPGESCTAPNCGRPRCGSAGILIENGKRLLALDGSAFP 194

BLAST of Cla019869 vs. TrEMBL
Match: B9SIQ2_RICCO (Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis GN=RCOM_0538680 PE=4 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.1e-41
Identity = 90/183 (49.18%), Postives = 119/183 (65.03%), Query Frame = 1

Query: 1   MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPV-T 60
           MA+++IAQ P V D+ GQPL  GVEYY+ P  TD  G LTL +R+ + CP +VGQEP+ T
Sbjct: 16  MAVSTIAQTPAVLDSSGQPLTSGVEYYVLPAATDTAGGLTLVNRTGS-CPFYVGQEPLPT 75

Query: 61  STNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGR 120
               G PV FTP+ AG+ II EG    + F A++ C+ ST W +   +++T RRF+  G 
Sbjct: 76  VAKTGFPVIFTPLAAGESIIREGMDFRVAFSAVTNCVQSTTWSIGDEDAETSRRFIVTGG 135

Query: 121 EDGPAGIFGISRDNGVYNIVWCPAMMG----RPRCGRAGILVENGVRLLALDGEAFPFEF 179
           E+     F I ++ G+YN+ WCP        RPRCG AGIL+ENG RLLALDG AFPF+F
Sbjct: 136 EE---DYFRIDKNEGLYNLGWCPNCNSINCPRPRCGFAGILIENGNRLLALDGAAFPFQF 194

BLAST of Cla019869 vs. TrEMBL
Match: D1KFM3_9ROSI (Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.0e-41
Identity = 97/188 (51.60%), Postives = 114/188 (60.64%), Query Frame = 1

Query: 1   MAITSIAQ-----FPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQ 60
           MAI S+AQ      PPV DT GQPL  GVEYYI P  TDV G LTL +R+   CP +VGQ
Sbjct: 15  MAICSLAQPADDQSPPVLDTSGQPLETGVEYYILPGITDVAGGLTLVNRNGIRCPFYVGQ 74

Query: 61  EPVTSTN-IGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRF 120
           EP+ S    G  V FTP  +G+ II E   L++ FQAL+ CI ST WRV   + +TGRRF
Sbjct: 75  EPLASAEPNGTSVIFTPYTSGETIIRESRDLSVQFQALTICIQSTAWRVGEEDPETGRRF 134

Query: 121 VGIGREDGPAGIFGISRDNGVYNIVWCPA----MMGRPRCGRAGILVENGVRLLALDGEA 179
           +      G    F I  + GVYN  WCP        RPRCG AGIL+E+  RLLALDG A
Sbjct: 135 IVTA---GDKSYFRIDNNGGVYNFSWCPTESCPNCARPRCGSAGILIEDDKRLLALDGPA 194

BLAST of Cla019869 vs. NCBI nr
Match: gi|449442847|ref|XP_004139192.1| (PREDICTED: miraculin-like [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 2.4e-87
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 1

Query: 1   MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 60
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 18  MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 77

Query: 61  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 120
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 78  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 137

Query: 121 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 179
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 138 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 195

BLAST of Cla019869 vs. NCBI nr
Match: gi|700205760|gb|KGN60879.1| (hypothetical protein Csa_2G021500 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 2.4e-87
Identity = 159/178 (89.33%), Postives = 165/178 (92.70%), Query Frame = 1

Query: 1   MAITSIAQFPPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPVTS 60
           MAITS AQ PPV DT+GQPL+RGVEYYISP  TDVGGNLTLKSRSNAPCPLFVGQEPVTS
Sbjct: 1   MAITSTAQLPPVLDTNGQPLQRGVEYYISPAITDVGGNLTLKSRSNAPCPLFVGQEPVTS 60

Query: 61  TNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIGRE 120
           TNIGLPVTF P   G DIIDEGTSLNIVFQALSTC+TSTQWRVDA ESDTGRRFVGIG E
Sbjct: 61  TNIGLPVTFRPTEEGIDIIDEGTSLNIVFQALSTCVTSTQWRVDATESDTGRRFVGIGDE 120

Query: 121 DGPAGIFGISRDNGVYNIVWCPAMMGRPRCGRAGILVENGVRLLALDGEAFPFEFVKA 179
           DGPAGIFGISRDNG YNIVWCPAMMGRPRCGRAGILVENGVRL+ALDG+AFPFEF+KA
Sbjct: 121 DGPAGIFGISRDNGAYNIVWCPAMMGRPRCGRAGILVENGVRLVALDGDAFPFEFIKA 178

BLAST of Cla019869 vs. NCBI nr
Match: gi|449442849|ref|XP_004139193.1| (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus])

HSP 1 Score: 204.5 bits (519), Expect = 1.5e-49
Identity = 104/183 (56.83%), Postives = 123/183 (67.21%), Query Frame = 1

Query: 1   MAITSIAQF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEPV 60
           MAI S AQF  PPV DT+G+PL+R VEY+I P  TDV GNLTL +R    CP +VGQ P+
Sbjct: 19  MAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPL 78

Query: 61  TSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGIG 120
            S  IG  VT TP + G D I EG  L  VFQA + CIT TQW+V   +  +GRRFV +G
Sbjct: 79  LSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKLG 138

Query: 121 REDGPAGIFGISRDN-GVYNIVWCPA---MMGRPRCGRAGILVENGVRLLALDGEAFPFE 178
            ++   G F I + N GVYNI WCP+     GRPRCG AGIL+E GVR LALDG AFPFE
Sbjct: 139 NDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPFE 198

BLAST of Cla019869 vs. NCBI nr
Match: gi|659070426|ref|XP_008455014.1| (PREDICTED: miraculin-like isoform X1 [Cucumis melo])

HSP 1 Score: 193.4 bits (490), Expect = 3.5e-46
Identity = 101/184 (54.89%), Postives = 121/184 (65.76%), Query Frame = 1

Query: 1   MAITSIA-QF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEP 60
           MAI S A QF  PPV DT+G+PL+R  EY+I P  TDV GNLTL +R    CP +VGQ P
Sbjct: 19  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 78

Query: 61  VTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGI 120
           + S   G  V+  P   G+D I EG  L  VFQ  + CIT TQW+V  A+  TGRRFV +
Sbjct: 79  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 138

Query: 121 GREDGPAGIFGISR-DNGVYNIVWCPA---MMGRPRCGRAGILVENGVRLLALDGEAFPF 178
           G ++   G F I + D GVYNI WCP+   + GRPRCG AGIL+E GVR LALDG AFPF
Sbjct: 139 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 198

BLAST of Cla019869 vs. NCBI nr
Match: gi|659070428|ref|XP_008455022.1| (PREDICTED: miraculin-like isoform X2 [Cucumis melo])

HSP 1 Score: 193.4 bits (490), Expect = 3.5e-46
Identity = 101/184 (54.89%), Postives = 121/184 (65.76%), Query Frame = 1

Query: 1   MAITSIA-QF--PPVFDTDGQPLRRGVEYYISPVFTDVGGNLTLKSRSNAPCPLFVGQEP 60
           MAI S A QF  PPV DT+G+PL+R  EY+I P  TDV GNLTL +R    CP +VGQ P
Sbjct: 18  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 77

Query: 61  VTSTNIGLPVTFTPIMAGKDIIDEGTSLNIVFQALSTCITSTQWRVDAAESDTGRRFVGI 120
           + S   G  V+  P   G+D I EG  L  VFQ  + CIT TQW+V  A+  TGRRFV +
Sbjct: 78  LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 137

Query: 121 GREDGPAGIFGISR-DNGVYNIVWCPA---MMGRPRCGRAGILVENGVRLLALDGEAFPF 178
           G ++   G F I + D GVYNI WCP+   + GRPRCG AGIL+E GVR LALDG AFPF
Sbjct: 138 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGPAFPF 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
MIRA_SYNDU8.1e-1936.32Miraculin OS=Synsepalum dulcificum PE=1 SV=3[more]
IAAS_HORVU1.0e-1339.17Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2[more]
IAAS_WHEAT1.3e-1340.83Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1[more]
IAAS_ORYSJ7.3e-1237.59Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 ... [more]
ASP_THECC6.9e-1032.4521 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LIK2_CUCSA1.7e-8789.33Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021500 PE=4 SV=1[more]
A0A0A0LGB4_CUCSA1.1e-4956.83Uncharacterized protein OS=Cucumis sativus GN=Csa_2G021000 PE=4 SV=1[more]
A0A0X7YK90_HEVBR8.1e-4251.61Alpha-amylase/subtilisin inhibitor OS=Hevea brasiliensis GN=ASI PE=2 SV=1[more]
B9SIQ2_RICCO1.1e-4149.18Alpha-amylase/subtilisin inhibitor, putative OS=Ricinus communis GN=RCOM_0538680... [more]
D1KFM3_9ROSI4.0e-4151.60Kunitz-type protease inhibitor KPI-F4 OS=Populus trichocarpa x Populus deltoides... [more]
Match NameE-valueIdentityDescription
gi|449442847|ref|XP_004139192.1|2.4e-8789.33PREDICTED: miraculin-like [Cucumis sativus][more]
gi|700205760|gb|KGN60879.1|2.4e-8789.33hypothetical protein Csa_2G021500 [Cucumis sativus][more]
gi|449442849|ref|XP_004139193.1|1.5e-4956.83PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis sativus][more]
gi|659070426|ref|XP_008455014.1|3.5e-4654.89PREDICTED: miraculin-like isoform X1 [Cucumis melo][more]
gi|659070428|ref|XP_008455022.1|3.5e-4654.89PREDICTED: miraculin-like isoform X2 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002160Prot_inh_Kunz-lg
IPR011065Kunitz_inhibitor_STI-like_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004866endopeptidase inhibitor activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
cellular_component GO:0005575 cellular_component
molecular_function GO:0004866 endopeptidase inhibitor activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla019869Cla019869.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002160Proteinase inhibitor I3, Kunitz legumePFAMPF00197Kunitz_legumecoord: 12..177
score: 1.2
IPR002160Proteinase inhibitor I3, Kunitz legumeSMARTSM00452kul_2coord: 11..178
score: 1.2
IPR002160Proteinase inhibitor I3, Kunitz legumePROSITEPS00283SOYBEAN_KUNITZcoord: 12..28
scor
IPR011065Kunitz inhibitor ST1-likeunknownSSF50386STI-likecoord: 10..178
score: 2.99
NoneNo IPR availableGENE3DG3DSA:2.80.10.50coord: 10..178
score: 6.9
NoneNo IPR availablePANTHERPTHR33107FAMILY NOT NAMEDcoord: 1..178
score: 6.3
NoneNo IPR availablePANTHERPTHR33107:SF4SUBFAMILY NOT NAMEDcoord: 1..178
score: 6.3