Cla016998 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla016998
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Ribonuclease S5 (Fragment) (AHRD V1 **** B1NSN8_PRUAV); contains Interpro domain(s) IPR001568 Ribonuclease T2
Location	Chr10 : 21195942 .. 21196744 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTCCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGGTAAAATTCATACACCCAAAGTCTCTAATTTTCTTGATTATTAATCATGCATCGATTCTAACTAAGAACCATTTTAAAATTAAGAAAAAGTTTATGATGCTAAAACATATTCATTTATAAGCTTAAAAGAAACACAAATATACTCAACATATAAGAATGTGTGGGCATTTCAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

mRNA sequence

ATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTCCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

Coding sequence (CDS)

ATGTTCTTTGTGTCATTTGTGTTAATCTTTTTGTTTCCTATTGTTAAATCACAAACTTTCGATAATTTTTGGTTTGTTCAACAATGGCCACCAGCTGTTTGTGCTTTACAATCTGGACGATGTGTAGGACGAGGGACTCGATCCTTTACAATCCATGGTTTGTGGCCTCAAAAGGGAGGAAACTCTGTAACTAATTGCGCTGGCACTCCATTTGATTTCACTAAGATTGCGCATTTAGAAAGCGATCTGAACATAGTTTGGACAAATGTGGTAACGGGAAACAACAAATTCTTTTGGGGTTACGAGTGGAATAAACATGGAATTTGCTCAGAAAGCAAGTTTGACATGGCGAAGTACTTCCAAACGGCGATAAACATGAGGCACAATATAGACCTTCTTAGCGTAATGAGAACTGGTGGAATTGTACCAAACGGAGCCTCAAAGGCGAAGCAAAGAGTAGAAAGTGCAATCTCATCCCATTTTGGTAAGGACCCAATTCTTCGGTGTAAAACAGCACCAAACGGCCAAGTTTTGTTGACAGAGATTGTTATGTGCTTTGATGACGATGGTGTCACCCTTCTACACTGCAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTGA

Protein sequence

MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDDGVTLLHCNKARSNCAGSFIF

Homology

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BLAST of Cla016998 vs. Swiss-Prot
Match: RNMC_MOMCH (Ribonuclease MC OS=Momordica charantia PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 6.7e-57
Identity = 102/193 (52.85%), Postives = 132/193 (68.39%), Query Frame = 1


Query: 20  FDNFWFVQQWPPAVCALQ-SGRCVGRGTRSFTIHGLWPQKGGNSVTNCA-GTPFDFTKIA 79
           FD+FWFVQQWPPAVC+ Q SG C G G R+FTIHGLWPQ  G S+TNC  G+PFD TKI+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 80  HLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHNIDLLSVMR 139
           HL+S LN +W NV+  NN+ FW +EW KHG CSES F+ A YF+ A++MR+N D++  +R
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 140 TGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQV-LLTEIVMCFDDDGVTLLHC 199
                PNG +K++Q ++  + + FGK P LRC+T P  +V  L ++V CF  DG TL+ C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 200 NKARSNCAGSFIF 210
              R  C  +FIF
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of Cla016998 vs. Swiss-Prot
Match: RNS1_ARATH (Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.8e-30
Identity = 69/187 (36.90%), Postives = 98/187 (52.41%), Query Frame = 1


Query: 17  SQTFDNFWFVQQWPPAVCALQSGRCV---GRGTRSFTIHGLWPQ-KGGNSVTNC-AGTPF 76
           S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K G   +NC A  PF
Sbjct: 27  SEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPF 86

Query: 77  DFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHN 136
           D + I+ L + +   W  +   +G+ + FW +EW KHG CSES  D  +YFQTA+N++  
Sbjct: 87  DSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVIDQHEYFQTALNLKQK 146

Query: 137 IDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDD 196
            +LL  +   GI P+G S + + +  +I    G  P + C    +G   L ++ +C D  
Sbjct: 147 TNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSGNSQLYQVYLCVDRS 206

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BLAST of Cla016998 vs. Swiss-Prot
Match: RNLE_SOLLC (Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2)

HSP 1 Score: 123.6 bits (309), Expect = 2.5e-27
Identity = 64/188 (34.04%), Postives = 101/188 (53.72%), Query Frame = 1


Query: 17  SQTFDNFWFVQQWPPAVCALQSGRC---VGRGTRSFTIHGLWPQKG-GNSVTNC-AGTPF 76
           ++ FD F+FVQQWP + C  +   C    G+    F IHGLWP    G   +NC   +P+
Sbjct: 26  AKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNNDGTYPSNCDPNSPY 85

Query: 77  DFTKIAHLESDLNIVWTNVV--TGNNKFFWGYEWNKHGICSESKF-DMAKYFQTAINMRH 136
           D ++I+ L S +   W  +   +G+   FW +EW KHG C+ES   +   YF+ A+++++
Sbjct: 86  DQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLTNQHAYFKKALDLKN 145

Query: 137 NIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDD 196
            IDLLS+++   I P+G S     + +AI S  G  P ++C    +G   L ++ +C D 
Sbjct: 146 QIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGYTPWIQCNVDQSGNSQLYQVYICVDG 205

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BLAST of Cla016998 vs. Swiss-Prot
Match: RNS3_ARATH (Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.3e-23
Identity = 66/205 (32.20%), Postives = 95/205 (46.34%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKS--QTFDNFWFVQQWPPAVCALQSGRC---VGRGTRSFTIHGLW 60
           M F  F+L      V+S  Q FD F+FV QWP A C  +   C    G+    F IHGLW
Sbjct: 1   MKFFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLW 60

Query: 61  PQ-KGGNSVTNC-AGTPFDFTKIAHLESDLNIVWTNVVTGNNKF--FWGYEWNKHGICSE 120
           P  K G    NC   + FD  +++ L SDL   W  +   +N    FW +EW KHG C+E
Sbjct: 61  PNYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAE 120

Query: 121 SKFDMAKYFQTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKT 180
           S+ D   YF+  + ++   +LL  +   GI P+      + +E+ I    G  P + C  
Sbjct: 121 SELDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNK 180

Query: 181 APNGQVLLTEIVMCFDDDGVTLLHC 197
             +    L +I +C D      ++C
Sbjct: 181 DSSHNSQLYQIYLCVDTSASKFINC 205

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BLAST of Cla016998 vs. Swiss-Prot
Match: RNDI_DICDI (Ribonuclease DdI OS=Dictyostelium discoideum GN=ddiA PE=1 SV=3)

HSP 1 Score: 106.3 bits (264), Expect = 4.1e-22
Identity = 66/207 (31.88%), Postives = 110/207 (53.14%), Query Frame = 1


Query: 9   IFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTR-SFTIHGLWPQKGGNSVTN-C 68
           + ++   K   FD + FVQQW  + C  Q+  C+    R +FTIHGLWP+    S  + C
Sbjct: 21  VTIYESSKPGDFDFYLFVQQWIYSYCDSQT--CIQNKEREAFTIHGLWPENSDGSYPSFC 80

Query: 69  AGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESK--FDMAKYFQTAI 128
           +G  F+   I  LE  LN  W + +TG N  FW +E++KHG CS +    D+  YF T I
Sbjct: 81  SGPSFNVNAIQDLEDQLNFDWPS-LTGPNTDFWTHEFSKHGTCSITGPITDIHDYFATGI 140

Query: 129 NMRHNIDLLSVMRTGGIVPNGASKAKQ-RVESAISSHFGKDPILRCKTAPNGQVLLTEIV 188
            +    ++ + + +  I P+ ++  K   + +AI++HFG  P ++C    +GQ  L+ + 
Sbjct: 141 KLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFGGKPGIQCS---SGQ--LSTVA 200

Query: 189 MCFDDDGVTLLHCNKARS-NCAGSFIF 210
           +C D + ++++ C   +  +C+GS  F
Sbjct: 201 VCIDKNSLSIMDCPDLQGWSCSGSVKF 219

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BLAST of Cla016998 vs. TrEMBL
Match: A0A0A0LC37_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 394.8 bits (1013), Expect = 6.5e-107
Identity = 185/209 (88.52%), Postives = 195/209 (93.30%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGG 60
           +FFV FVL  LFPIVKSQTFD+FWFVQQWPPAVC LQSGRCVGRGTRSFTIHGLWPQKGG
Sbjct: 10  VFFV-FVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKGG 69

Query: 61  NSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYF 120
            SVTNC G  FDFTKIAHLE+DLN+VW NVVTGNNKFFWG+EWNKHGICSESKFD AKYF
Sbjct: 70  RSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYF 129

Query: 121 QTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 180
           QTAINMRH IDLLSV+RTGG+ PNGASKAKQRVE+AISSHFGKDPILRCK A NGQVLLT
Sbjct: 130 QTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLT 189

Query: 181 EIVMCFDDDGVTLLHCNKARSNCAGSFIF 210
           EIVMCFDDDGVTL++CNKARSNCAGSFIF
Sbjct: 190 EIVMCFDDDGVTLINCNKARSNCAGSFIF 217

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BLAST of Cla016998 vs. TrEMBL
Match: Q40115_LUFAE (Ribonuclease (RNase LC1) OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 2.9e-83
Identity = 144/204 (70.59%), Postives = 166/204 (81.37%), Query Frame = 1


Query: 6   FVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSVTN 65
           FVL  LFP+VKSQTFD+FW VQ WPPAVC+ Q GRCVG+G RSFTIHG+WPQKGG SV N
Sbjct: 12  FVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQGLRSFTIHGVWPQKGGTSVIN 71

Query: 66  CAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAIN 125
           C G  FDFTKI+HLES LN+ W NV+TGNNK+FWG+EWNKHGICS SKFD   YFQ AIN
Sbjct: 72  CPGPTFDFTKISHLESTLNVDWPNVITGNNKWFWGHEWNKHGICSVSKFDQQAYFQMAIN 131

Query: 126 MRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMC 185
           MR++IDLLS +R GG+VPNG SKA+QRV+SAI +  GK+P+LRC+     Q  L EIVMC
Sbjct: 132 MRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCR-GTGRQSRLLEIVMC 191

Query: 186 FDDDGVTLLHCNKARSNCAGSFIF 210
           FDDDGVTL++CN A SNC  SFIF
Sbjct: 192 FDDDGVTLINCNPANSNCPNSFIF 214

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BLAST of Cla016998 vs. TrEMBL
Match: A0A0A0LHQ9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825060 PE=3 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-66
Identity = 117/209 (55.98%), Postives = 155/209 (74.16%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGG 60
           + FV  + I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKGG
Sbjct: 13  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 72

Query: 61  NSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYF 120
            SV NC GT FDF KI+ L + L+ +  +V+  +++F W +EWNKHG+CSES++ M +YF
Sbjct: 73  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 132

Query: 121 QTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 180
           Q AINM++ I++LS +R GGI PN   KAKQRVE A+ + +   P+LRCK   +GQ LLT
Sbjct: 133 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLLT 192

Query: 181 EIVMCFDDDGVTLLHCNKARSNCAGSFIF 210
           E+VMCFD+DGVTLL+C   +SNC    +F
Sbjct: 193 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 221

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BLAST of Cla016998 vs. TrEMBL
Match: Q40116_LUFAE (Ribonuclease (RNase LC2) OS=Luffa aegyptiaca PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 2.3e-51
Identity = 101/206 (49.03%), Postives = 134/206 (65.05%), Query Frame = 1


Query: 4   VSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSV 63
           +  +L  LF   +SQTFD+F+FVQQWPP  C  Q   C      +F IHGLWPQKG NSV
Sbjct: 11  IVLLLTILFLGAESQTFDHFFFVQQWPPTTCQQQQKPCFQPPPATFKIHGLWPQKGPNSV 70

Query: 64  TNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTA 123
             C    FD T+I+ LE+ L++VW +VVTGNN  FW +EWNKHG CSES+F+   YFQTA
Sbjct: 71  VYC-NKNFDRTQISSLENQLDVVWPDVVTGNNTGFWEHEWNKHGSCSESQFNQTLYFQTA 130

Query: 124 INMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIV 183
           INM + ++LL  +  GGI  +  +K+ Q ++  + + FG  P LRCK     Q  L EIV
Sbjct: 131 INMMNKVNLLKALGKGGITSDERTKSSQTMQKVLLAQFGNQPFLRCKKV-GQQFWLLEIV 190

Query: 184 MCFDDDGVTLLHCNKARSNCAGSFIF 210
           MCF DDGVT+++C  ++ +C  +FIF
Sbjct: 191 MCFKDDGVTMINCQPSKVSCPPNFIF 214

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BLAST of Cla016998 vs. TrEMBL
Match: A9XXE8_PRUMU (S14-RNase OS=Prunus mume PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-32
Identity = 79/212 (37.26%), Postives = 115/212 (54.25%), Query Frame = 1


Query: 2   FFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGN 61
           F V     F+  ++ S ++D F FVQQWPP  C L++     R  ++FTIHGLWP    N
Sbjct: 10  FLVLVFAFFVCYVMSSGSYDYFQFVQQWPPTNCILRTKCSKPRPLQNFTIHGLWPSNYSN 69

Query: 62  SV--TNCAGTPFDFTKIA-HLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAK 121
               +NC G+ F+FTK++  L S L I W +V +GN+  FW  EWNKHG CSE   +  +
Sbjct: 70  PKMPSNCIGSRFNFTKVSPRLRSKLKISWPDVESGNDTKFWEGEWNKHGTCSERTLNQMQ 129

Query: 122 YFQTAINMRHNIDLLSVMRTGGIVPNGASKAK-QRVESAISSHFGKDPILRCKTAPNGQV 181
           YFQ +  M +  ++  ++R   IVP+     K   + S I +   + P+LRCK  P  Q 
Sbjct: 130 YFQRSHEMWNFHNITEILRNASIVPHATHTWKYSDIVSPIQTAIKRTPLLRCKPRPKSQT 189

Query: 182 -----------LLTEIVMCFDDDGVTLLHCNK 199
                      LL E+V+CFD D + ++ CN+
Sbjct: 190 KSQPKSQAMSQLLHEVVLCFDYDALVIIDCNR 221

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BLAST of Cla016998 vs. NCBI nr
Match: gi|659085407|ref|XP_008443402.1| (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 397.5 bits (1020), Expect = 1.4e-107
Identity = 186/209 (89.00%), Postives = 197/209 (94.26%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGG 60
           +FFV FVL  LFPIVKSQTFDNFWFVQQWPPA+C LQSGRCVGRGTRSFTIHGLWPQKGG
Sbjct: 9   VFFV-FVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGG 68

Query: 61  NSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYF 120
            SVTNC+G  FDFTKIAHLESDLN+VW NVVTGNNKFFWG+EWNKHGICSESKFD AKYF
Sbjct: 69  RSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDQAKYF 128

Query: 121 QTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 180
           QTAINMRH+IDLLSV+RTGGIVPNGASKAKQRVE+AISSHF KDPILRCKTA NGQVLLT
Sbjct: 129 QTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTASNGQVLLT 188

Query: 181 EIVMCFDDDGVTLLHCNKARSNCAGSFIF 210
           EIV+CFDDDGVTL++CNKARSNCA SFIF
Sbjct: 189 EIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of Cla016998 vs. NCBI nr
Match: gi|449459530|ref|XP_004147499.1| (PREDICTED: ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 394.8 bits (1013), Expect = 9.3e-107
Identity = 185/209 (88.52%), Postives = 195/209 (93.30%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGG 60
           +FFV FVL  LFPIVKSQTFD+FWFVQQWPPAVC LQSGRCVGRGTRSFTIHGLWPQKGG
Sbjct: 10  VFFV-FVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIHGLWPQKGG 69

Query: 61  NSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYF 120
            SVTNC G  FDFTKIAHLE+DLN+VW NVVTGNNKFFWG+EWNKHGICSESKFD AKYF
Sbjct: 70  RSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYF 129

Query: 121 QTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 180
           QTAINMRH IDLLSV+RTGG+ PNGASKAKQRVE+AISSHFGKDPILRCK A NGQVLLT
Sbjct: 130 QTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLT 189

Query: 181 EIVMCFDDDGVTLLHCNKARSNCAGSFIF 210
           EIVMCFDDDGVTL++CNKARSNCAGSFIF
Sbjct: 190 EIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of Cla016998 vs. NCBI nr
Match: gi|976231|dbj|BAA10891.1| (ribonuclease (RNase LC1) [Luffa aegyptiaca])

HSP 1 Score: 316.2 bits (809), Expect = 4.2e-83
Identity = 144/204 (70.59%), Postives = 166/204 (81.37%), Query Frame = 1


Query: 6   FVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSVTN 65
           FVL  LFP+VKSQTFD+FW VQ WPPAVC+ Q GRCVG+G RSFTIHG+WPQKGG SV N
Sbjct: 12  FVLTILFPMVKSQTFDSFWMVQHWPPAVCSFQQGRCVGQGLRSFTIHGVWPQKGGTSVIN 71

Query: 66  CAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAIN 125
           C G  FDFTKI+HLES LN+ W NV+TGNNK+FWG+EWNKHGICS SKFD   YFQ AIN
Sbjct: 72  CPGPTFDFTKISHLESTLNVDWPNVITGNNKWFWGHEWNKHGICSVSKFDQQAYFQMAIN 131

Query: 126 MRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMC 185
           MR++IDLLS +R GG+VPNG SKA+QRV+SAI +  GK+P+LRC+     Q  L EIVMC
Sbjct: 132 MRNSIDLLSALRVGGVVPNGRSKARQRVQSAIRAQLGKEPVLRCR-GTGRQSRLLEIVMC 191

Query: 186 FDDDGVTLLHCNKARSNCAGSFIF 210
           FDDDGVTL++CN A SNC  SFIF
Sbjct: 192 FDDDGVTLINCNPANSNCPNSFIF 214

[Close]

BLAST of Cla016998 vs. NCBI nr
Match: gi|700204428|gb|KGN59561.1| (hypothetical protein Csa_3G825060 [Cucumis sativus])

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-66
Identity = 117/209 (55.98%), Postives = 155/209 (74.16%), Query Frame = 1


Query: 1   MFFVSFVLIFLFPIVKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGG 60
           + FV  + I LFPI+KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKGG
Sbjct: 13  LLFVVTLSISLFPIMKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGG 72

Query: 61  NSVTNCAGTPFDFTKIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYF 120
            SV NC GT FDF KI+ L + L+ +  +V+  +++F W +EWNKHG+CSES++ M +YF
Sbjct: 73  KSVINCPGTQFDFNKISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYF 132

Query: 121 QTAINMRHNIDLLSVMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLT 180
           Q AINM++ I++LS +R GGI PN   KAKQRVE A+ + +   P+LRCK   +GQ LLT
Sbjct: 133 QMAINMKYKINVLSALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLLT 192

Query: 181 EIVMCFDDDGVTLLHCNKARSNCAGSFIF 210
           E+VMCFD+DGVTLL+C   +SNC    +F
Sbjct: 193 EVVMCFDNDGVTLLNCTTTKSNCDADVLF 221

[Close]

BLAST of Cla016998 vs. NCBI nr
Match: gi|778685547|ref|XP_004147497.2| (PREDICTED: ribonuclease MC-like [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 6.3e-63
Identity = 110/195 (56.41%), Postives = 146/195 (74.87%), Query Frame = 1


Query: 15  VKSQTFDNFWFVQQWPPAVCALQSGRCVGRGTRSFTIHGLWPQKGGNSVTNCAGTPFDFT 74
           +KSQ FD F+FVQQWPPAVC  ++G+CVG+G   FTIHG+WPQKGG SV NC GT FDF 
Sbjct: 1   MKSQQFDKFYFVQQWPPAVCDGRTGKCVGKGMYYFTIHGVWPQKGGKSVINCPGTQFDFN 60

Query: 75  KIAHLESDLNIVWTNVVTGNNKFFWGYEWNKHGICSESKFDMAKYFQTAINMRHNIDLLS 134
           KI+ L + L+ +  +V+  +++F W +EWNKHG+CSES++ M +YFQ AINM++ I++LS
Sbjct: 61  KISSLANTLHQIMKDVINADDQFLWSHEWNKHGVCSESRYSMKQYFQMAINMKYKINVLS 120

Query: 135 VMRTGGIVPNGASKAKQRVESAISSHFGKDPILRCKTAPNGQVLLTEIVMCFDDDGVTLL 194
            +R GGI PN   KAKQRVE A+ + +   P+LRCK   +GQ LLTE+VMCFD+DGVTLL
Sbjct: 121 ALRMGGITPNNHLKAKQRVEGAMFTAYNAYPLLRCKKDSSGQSLLTEVVMCFDNDGVTLL 180

Query: 195 HCNKARSNCAGSFIF 210
           +C   +SNC    +F
Sbjct: 181 NCTTTKSNCDADVLF 195

The following BLAST results are available for this feature:

BLAST of Cla016998 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RNMC_MOMCH	6.7e-57	52.85	Ribonuclease MC OS=Momordica charantia PE=1 SV=1	[more]
RNS1_ARATH	1.8e-30	36.90	Ribonuclease 1 OS=Arabidopsis thaliana GN=RNS1 PE=1 SV=1	[more]
RNLE_SOLLC	2.5e-27	34.04	Extracellular ribonuclease LE OS=Solanum lycopersicum PE=1 SV=2	[more]
RNS3_ARATH	8.3e-23	32.20	Ribonuclease 3 OS=Arabidopsis thaliana GN=RNS3 PE=2 SV=1	[more]
RNDI_DICDI	4.1e-22	31.88	Ribonuclease DdI OS=Dictyostelium discoideum GN=ddiA PE=1 SV=3	[more]

BLAST of Cla016998 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC37_CUCSA	6.5e-107	88.52	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825050 PE=3 SV=1	[more]
Q40115_LUFAE	2.9e-83	70.59	Ribonuclease (RNase LC1) OS=Luffa aegyptiaca PE=2 SV=1	[more]
A0A0A0LHQ9_CUCSA	1.5e-66	55.98	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G825060 PE=3 SV=1	[more]
Q40116_LUFAE	2.3e-51	49.03	Ribonuclease (RNase LC2) OS=Luffa aegyptiaca PE=2 SV=1	[more]
A9XXE8_PRUMU	2.3e-32	37.26	S14-RNase OS=Prunus mume PE=2 SV=1	[more]

BLAST of Cla016998 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659085407\|ref\|XP_008443402.1\|	1.4e-107	89.00	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
gi\|449459530\|ref\|XP_004147499.1\|	9.3e-107	88.52	PREDICTED: ribonuclease MC-like [Cucumis sativus]	[more]
gi\|976231\|dbj\|BAA10891.1\|	4.2e-83	70.59	ribonuclease (RNase LC1) [Luffa aegyptiaca]	[more]
gi\|700204428\|gb\|KGN59561.1\|	2.1e-66	55.98	hypothetical protein Csa_3G825060 [Cucumis sativus]	[more]
gi\|778685547\|ref\|XP_004147497.2\|	6.3e-63	56.41	PREDICTED: ribonuclease MC-like [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001568	RNase_T2-like
IPR018188	RNase_T2_His_AS_1

Vocabulary: **Molecular Function**
Term	Definition
GO:0003723	RNA binding
GO:0033897	ribonuclease T2 activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU51257	watermelon EST collection version 2.0	transcribed_cluster
WMU52371	watermelon EST collection version 2.0	transcribed_cluster
WMU57365	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla016998	Cla016998.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU52371	WMU52371	transcribed_cluster
WMU57365	WMU57365	transcribed_cluster
WMU51257	WMU51257	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	GENE3D	G3DSA:3.90.730.10		coord: 20..208 score: 1.8
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 1..190 score: 4.2
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 23..196 score: 1.4
IPR001568	Ribonuclease T2-like	unknown	SSF55895	Ribonuclease Rh-like	coord: 20..209 score: 9.55
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 49..56 scor

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla016998	Csa3G825060	Cucumber (Chinese Long) v2	cuwmB198
Cla016998	Csa5G636550	Cucumber (Chinese Long) v2	cuwmB368
Cla016998	Cucsa.285040	Cucumber (Gy14) v1	cgywmB520

The following gene(s) are paralogous to this gene:


Gene	Paralogue	Organism	Block
Cla016998	Cla010187	Watermelon (97103) v1	wmwmB045

The following block(s) are covering this gene:


Gene	Organism	Block
Cla016998	Cucumber (Chinese Long) v3	cucwmB208
Cla016998	Cucumber (Chinese Long) v3	cucwmB393
Cla016998	Cucumber (Chinese Long) v3	cucwmB397
Cla016998	Watermelon (97103) v2	wmwmbB051
Cla016998	Watermelon (97103) v2	wmwmbB076
Cla016998	Wax gourd	wgowmB021
Cla016998	Wax gourd	wgowmB122
Cla016998	Watermelon (97103) v1	wmwmB009
Cla016998	Cucumber (Gy14) v1	cgywmB281
Cla016998	Cucumber (Gy14) v1	cgywmB609
Cla016998	Cucurbita maxima (Rimu)	cmawmB089
Cla016998	Cucurbita maxima (Rimu)	cmawmB228
Cla016998	Cucurbita maxima (Rimu)	cmawmB257
Cla016998	Cucurbita maxima (Rimu)	cmawmB658
Cla016998	Cucurbita moschata (Rifu)	cmowmB080
Cla016998	Cucurbita moschata (Rifu)	cmowmB214
Cla016998	Cucurbita moschata (Rifu)	cmowmB652
Cla016998	Wild cucumber (PI 183967)	cpiwmB198
Cla016998	Wild cucumber (PI 183967)	cpiwmB376
Cla016998	Wild cucumber (PI 183967)	cpiwmB379
Cla016998	Melon (DHL92) v3.5.1	mewmB008
Cla016998	Melon (DHL92) v3.5.1	mewmB045
Cla016998	Melon (DHL92) v3.5.1	mewmB346
Cla016998	Watermelon (Charleston Gray)	wcgwmB018
Cla016998	Watermelon (Charleston Gray)	wcgwmB051
Cla016998	Watermelon (Charleston Gray)	wcgwmB272
Cla016998	Cucumber (Chinese Long) v2	cuwmB371
Cla016998	Cucurbita pepo (Zucchini)	cpewmB167
Cla016998	Cucurbita pepo (Zucchini)	cpewmB597
Cla016998	Cucurbita pepo (Zucchini)	cpewmB641
Cla016998	Bottle gourd (USVL1VR-Ls)	lsiwmB181
Cla016998	Bottle gourd (USVL1VR-Ls)	lsiwmB240
Cla016998	Cucumber (Gy14) v2	cgybwmB187
Cla016998	Cucumber (Gy14) v2	cgybwmB347
Cla016998	Melon (DHL92) v3.6.1	medwmB006
Cla016998	Melon (DHL92) v3.6.1	medwmB047
Cla016998	Melon (DHL92) v3.6.1	medwmB344
Cla016998	Silver-seed gourd	carwmB0201
Cla016998	Silver-seed gourd	carwmB0249
Cla016998	Silver-seed gourd	carwmB0431