Cla016577 (gene) Watermelon (97103) v1

BLAST of Cla016577 vs. Swiss-Prot
Match: DRTS_DAUCA (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota PE=2 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 1.9e-188
Identity = 326/462 (70.56%), Postives = 366/462 (79.22%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS+ SALELLA SPY
Sbjct: 67  NAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALELLAGSPY 126

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
           C+S+EKVFVIGGG+I REALNAP CDA+HITEIE ++ECDTF+P +D+S+FQPWYSSFP+
Sbjct: 127 CVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSFPL 186

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
           VEN +RY FT+YVRVR+S      Q NG  S  K D  NFE + FSFLPK +FE+HEEYL
Sbjct: 187 VENKIRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTVFEKHEEYL 246

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC------------- 356
           YL LV+ IIS+G+ K+DRT TGT+S FGCQMR+NLRK+FPLLTTK               
Sbjct: 247 YLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTKKVFWRGVVEELLWFI 306

Query: 357 -----AGIL-------------------IGLREREEGDLGPVYGFQWRHFGARYTDMHAD 416
                A IL                   IGL +REEGDLGP+YGFQWRHFGARYTDMHAD
Sbjct: 307 SGSTNAKILKEKGVNIWEGNGSREYLDSIGLTDREEGDLGPIYGFQWRHFGARYTDMHAD 366

Query: 417 YTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQM 476
           Y+GQGFDQLLDVI+KIKNNPDDRRII SAWNPSDL+LMALPPCHMFAQFYVANGELSCQM
Sbjct: 367 YSGQGFDQLLDVISKIKNNPDDRRIIQSAWNPSDLRLMALPPCHMFAQFYVANGELSCQM 426

Query: 477 YQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQE-QLQK 536
           YQRSADMGLGVPFNIA+YALLTCMIAHVC L  GDF+H IGDAHVY  H+  L E   + 
Sbjct: 427 YQRSADMGLGVPFNIAAYALLTCMIAHVCDLVPGDFVHSIGDAHVYSNHLSDLFETSFRM 486

Query: 537 LPKPFPILKINPEKKDIDSFVADDFELTGYDPHEKIAMKMAV 541
           LPK FP+LKIN  +KDIDSF A DF+L GYDPH+KI MKMAV
Sbjct: 487 LPKTFPVLKINSGEKDIDSFEAADFKLIGYDPHQKIEMKMAV 528

HSP 2 Score: 372.5 bits (955), Expect = 8.0e-102
Identity = 194/341 (56.89%), Postives = 230/341 (67.45%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MA + L +    + +  P DP+RTYQVVVAAT+NMGIGKDGKLPWRLPSD+ FFK++T T
Sbjct: 1   MASELLANPTNGSGITRP-DPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD  KRNAV+MGRKTWESIP +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS+ SALE
Sbjct: 61  TSDPLKRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALE 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           +                         +GS    +VE V + G      E L         
Sbjct: 121 L------------------------LAGSPYCVSVEKVFVIGGGQIYREALN-------- 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
                            AP CDA+HITEIE ++ECDTF+P +D+S+FQPWYSSFP+VEN 
Sbjct: 181 -----------------APGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSFPLVENK 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +RY FT+YVRVR+S      Q NG  S  K D  NFE + FSFLPK +FE+HEEYLYL L
Sbjct: 241 IRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTVFEKHEEYLYLGL 291

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTK 342
           V+ IIS+G+ K+DRT TGT+S FGCQMR+NLRK+FPLLTTK
Sbjct: 301 VENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTK 291

BLAST of Cla016577 vs. Swiss-Prot
Match: DRTS2_ARATH (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana GN=THY-2 PE=2 SV=2)

HSP 1 Score: 375.9 bits (964), Expect = 7.2e-103
Identity = 175/193 (90.67%), Postives = 182/193 (94.30%), Query Frame = 1

Query: 348 IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILS 407
           IGL EREEGDLGPVYGFQWRHFGA+YTDMHADYTGQGFDQLLDVINKIKNNPDDRRII+S
Sbjct: 373 IGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMS 432

Query: 408 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 467
           AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASY+LLTC++AHV
Sbjct: 433 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHV 492

Query: 468 CGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTG 527
           C L  GDFIHVIGDAHVY+ HVRPLQEQL+  PKPFP+LKINPEKKDIDSFVADDFEL G
Sbjct: 493 CDLVPGDFIHVIGDAHVYKNHVRPLQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIG 552

Query: 528 YDPHEKIAMKMAV 541
           YDPH+KI MKMAV
Sbjct: 553 YDPHKKIDMKMAV 565

HSP 2 Score: 299.3 bits (765), Expect = 8.6e-80
Identity = 146/225 (64.89%), Postives = 177/225 (78.67%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL+LLAA P+
Sbjct: 109 NAVVMGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPF 168

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
            LSIEKVFVIGGG+ILREALN P C+AIHITEI+T+++CDTF+P +D S +QPW SSFP+
Sbjct: 169 SLSIEKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPI 228

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
            EN LR+SFT++VRV+SS+       +      + D+  F     S LPK IF+RHEEYL
Sbjct: 229 CENGLRFSFTTHVRVKSSSAGEASDESDGSKVLQVDWKKFS----SVLPKMIFDRHEEYL 288

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTK 342
           YLNLV+EIIS+G  KDDRTGTGTLS+FGCQM++NLR+ FPLLTTK
Sbjct: 289 YLNLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTK 329

HSP 3 Score: 157.9 bits (398), Expect = 3.1e-37
Identity = 86/173 (49.71%), Postives = 108/173 (62.43%), Query Frame = 1

Query: 12  NANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTTTSDHGKRNAVL 71
           N NV     P+ TYQVVVAAT+ MGIGKDGKLPW LP+DL FFK++T +TSD  K+NAV+
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALEMGRKTWESIPA 131
           MGRKTWESIP ++RPL GRLNVVL+RS  FDIA  ENV+ C SI SAL++      S+  
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EHRPLPGR---LNVVLTRSG--SFDIATVENVIICGSITSALELLAASPYCLS 180
           E   + G    L   L +    +  I  ++  I C +    ++  A  P+C S
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSS 225

BLAST of Cla016577 vs. Swiss-Prot
Match: DRTS1_ARATH (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana GN=THY-1 PE=1 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 3.0e-101
Identity = 171/193 (88.60%), Postives = 181/193 (93.78%), Query Frame = 1

Query: 348 IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILS 407
           IGL EREEGDLGPVYGFQWRHFGA+YTDMHADYTGQGFDQL+DVI+KIKNNPDDRRII+S
Sbjct: 327 IGLTEREEGDLGPVYGFQWRHFGAKYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMS 386

Query: 408 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 467
           AWNPSDLKLMALPPCHMFAQFYVA GELSCQMYQRSADMGLGVPFNIASY+LLTCM+AHV
Sbjct: 387 AWNPSDLKLMALPPCHMFAQFYVAEGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHV 446

Query: 468 CGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTG 527
           C L  GDFIHV+GDAHVY+THVRPLQEQL  LPKPFP++KINPEKK IDSFVA DF+LTG
Sbjct: 447 CDLVPGDFIHVLGDAHVYKTHVRPLQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTG 506

Query: 528 YDPHEKIAMKMAV 541
           YDPH+KI MKMAV
Sbjct: 507 YDPHKKIEMKMAV 519

HSP 2 Score: 322.8 bits (826), Expect = 7.2e-87
Identity = 163/286 (56.99%), Postives = 209/286 (73.08%), Query Frame = 1

Query: 56  NITTTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSI 115
           ++TTT +   +R   ++   T E    +   LP  L   L       + T ++     S 
Sbjct: 9   SVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDS-----SK 68

Query: 116 TSALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASP 175
            +A+ MGRKTWESIP ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 69  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 128

Query: 176 YCLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFP 235
           YCLSIE+VFVIGGG+ILREALN P CDAIH+TEI+T+V+CDTF+PAID S++QPW SSFP
Sbjct: 129 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 188

Query: 236 VVENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEY 295
           V EN LR+ FT++VRV+SS +   D++NG  S        F+ KKF FLPK +F++HEE+
Sbjct: 189 VTENGLRFCFTTFVRVKSSADESSDESNGSQS------LQFDGKKFLFLPKMVFDQHEEF 248

Query: 296 LYLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTK 342
           LYLN+V++IIS+G  K+DRTGTGTLS+FGCQM++NLR++FPLLTTK
Sbjct: 249 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTK 283

HSP 3 Score: 164.1 bits (414), Expect = 4.3e-39
Identity = 77/115 (66.96%), Postives = 90/115 (78.26%), Query Frame = 1

Query: 7   TSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTTTSDHGK 66
           T+ N +       +P+RTYQVVVAAT+ MGIGKDGKLPW LP+DL FFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALEM 122
           +NAV+MGRKTWESIP ++RPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL++
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDL 118

BLAST of Cla016577 vs. Swiss-Prot
Match: DRTS_SOYBN (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max PE=1 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 3.1e-98
Identity = 168/193 (87.05%), Postives = 176/193 (91.19%), Query Frame = 1

Query: 348 IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILS 407
           +GL EREEGDLGPVYGFQWRHFGARYTDMH DY+GQGFDQLLDVINKIK NPDDRRIILS
Sbjct: 338 VGLTEREEGDLGPVYGFQWRHFGARYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILS 397

Query: 408 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 467
           AWNP DLKLMALPPCHMFAQFYVA+GELSCQMYQRSADMGLG+PFNIASYALLTCMIAHV
Sbjct: 398 AWNPVDLKLMALPPCHMFAQFYVAHGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHV 457

Query: 468 CGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTG 527
           C L  GDFIHVIGDAH+YR HVRPLQEQL   PKPFP LKINP+KKDIDSFVA DF+L G
Sbjct: 458 CDLIPGDFIHVIGDAHIYRNHVRPLQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIG 517

Query: 528 YDPHEKIAMKMAV 541
           YDPH+KI MK++V
Sbjct: 518 YDPHQKIDMKLSV 530

HSP 2 Score: 327.8 bits (839), Expect = 2.3e-88
Identity = 160/225 (71.11%), Postives = 184/225 (81.78%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP E+RPL GRLNVVLTRSGSFDIAT ENV+ICGS++SALELLAASPY
Sbjct: 70  NAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSALELLAASPY 129

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
            LSIEKVFVIGGG+I REALN P C+AIH+TEI++++ECDTFMP +D ++F+PWYSSFP 
Sbjct: 130 SLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIFRPWYSSFPK 189

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
           VEN++RYSFT+YVRVRSS      QN         +   FE K FSFLPK I ERHEEYL
Sbjct: 190 VENNIRYSFTTYVRVRSSAAESAGQNIDPLLDNNSESMKFEVKDFSFLPKMISERHEEYL 249

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTK 342
           YL LVQ+II++G  K DRTGTGTLS+FGCQMR+NLR  FPLLTTK
Sbjct: 250 YLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPLLTTK 294

HSP 3 Score: 176.4 bits (446), Expect = 8.4e-43
Identity = 87/123 (70.73%), Postives = 101/123 (82.11%), Query Frame = 1

Query: 1   MAGDPLTSSNGNAN--VNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNIT 60
           M  D    SNG++N  VN  P+ +RTYQVVVAAT++ GIGKDGKLPWRLP+DL FFK IT
Sbjct: 1   MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEIT 60

Query: 61  TTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSA 120
             TS+ GK+NA++MGRKTWESIP E+RPL GRLNVVLTRSGSFDIAT ENV+ICGS++SA
Sbjct: 61  MKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSA 120

Query: 121 LEM 122
           LE+
Sbjct: 121 LEL 123

BLAST of Cla016577 vs. Swiss-Prot
Match: DRTS_PARTE (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Paramecium tetraurelia GN=GSPATT00019973001 PE=3 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 5.3e-98
Identity = 196/457 (42.89%), Postives = 265/457 (57.99%), Query Frame = 1

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           MGRKT+ES+ +  +PLP R+NVV++++    + +   V +   I  AL+      Y  S 
Sbjct: 47  MGRKTFESMNS--KPLPNRVNVVISKN----MKSSNEVQVFPRIEDALQF-----YNTSH 106

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
           +K+++IGG  I  E L   +C  +H+T I    +CD ++     S FQ   +S    EN 
Sbjct: 107 QKLYLIGGKRIFEEGLATDKCSDVHLTRIGVETKCDVYLNKNIFSTFQVNKTSQTKSENG 166

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           + Y +   +   S  +S ID                             E H+E  YL++
Sbjct: 167 INYDYQHLINKNSHEQSYID-----------------------------EEHQENQYLDM 226

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC--AGIL----------- 360
           + +I+ +G+ KDDRTG GT+S FG  MR+NL ++FPLLTTK     G++           
Sbjct: 227 ITKIMKEGVSKDDRTGVGTMSIFGQTMRFNLAQSFPLLTTKKVFFRGVVEELLWFLRGNT 286

Query: 361 ------------------------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQ 420
                                   IGL+ R+E DLGPVYGFQWRHFGA+Y D   DY+ Q
Sbjct: 287 NGKLLLDKGVKIWEGNGTREYLDTIGLQHRQEHDLGPVYGFQWRHFGAKYKDCQTDYSNQ 346

Query: 421 GFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS 480
           G DQ+ ++I  +KNNPD RRIILSAWNP DLK MALPPCH+ +QF+VANG+LSC MYQRS
Sbjct: 347 GVDQVKEIIQLLKNNPDSRRIILSAWNPIDLKQMALPPCHVMSQFFVANGKLSCMMYQRS 406

Query: 481 ADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPF 540
            D GLG+PFNIASYALLT M+A  C L  G+F+HV+GD H+Y  HV  L++Q++++P PF
Sbjct: 407 CDFGLGIPFNIASYALLTYMLAKECNLNLGEFVHVLGDTHIYSNHVEALKKQIERVPYPF 462

HSP 2 Score: 63.5 bits (153), Expect = 7.9e-09
Identity = 34/76 (44.74%), Postives = 47/76 (61.84%), Query Frame = 1

Query: 23 RTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTTTSDHGKRNAVLMGRKTWESIPA 82
          +T+ +V+A T N GIG   +LPW+L  DL  FK ITT          V+MGRKT+ES+  
Sbjct: 5  KTFSMVLAMTLNGGIGYQNRLPWKLKEDLQRFKKITT-------GGIVIMGRKTFESM-- 64

Query: 83 EHRPLPGRLNVVLTRS 99
            +PLP R+NVV++++
Sbjct: 65 NSKPLPNRVNVVISKN 71

BLAST of Cla016577 vs. TrEMBL
Match: W9SFP6_9ROSA (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Morus notabilis GN=L484_008752 PE=3 SV=1)

HSP 1 Score: 702.6 bits (1812), Expect = 3.7e-199
Identity = 367/576 (63.72%), Postives = 412/576 (71.53%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MAG+ + ++NGN NV   P  +RTYQVV AAT +MGIGKDGKLPWRLPSDL FFK IT T
Sbjct: 1   MAGENVITANGNGNVQTIP--QRTYQVVAAATRDMGIGKDGKLPWRLPSDLKFFKEITMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD GK+NAVLMGRKTWESIP EHRPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SALE
Sbjct: 61  TSDTGKKNAVLMGRKTWESIPLEHRPLPGRLNVVLTRSGSFDIATAENVLICGSMASALE 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           +                         + S    ++E V + G      E L AS      
Sbjct: 121 L------------------------LAASPYCLSIERVFVIGGGQILREALNASS----- 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
                               CDA+HITEIET++ECDTF+PAID S FQPWYSSFPVVEN+
Sbjct: 181 --------------------CDAVHITEIETSIECDTFIPAIDSSAFQPWYSSFPVVENN 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +R+SFT+YVRVRS      ++N  L    +PDFS FE KKF FLPK IF+RHEEY+YL L
Sbjct: 241 IRFSFTTYVRVRSPAIESPNENGDLAVDSEPDFSKFEIKKFFFLPKMIFDRHEEYVYLRL 300

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL---------- 360
           VQ+IISDGI KDDRTGTGT S+FGCQ    R  + +    ++    + +L          
Sbjct: 301 VQDIISDGISKDDRTGTGTFSKFGCQRLFWRGIVEELLWFISGSTSSKVLKDKGISIWDG 360

Query: 361 ---------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNN 420
                    IGL++REEGDLGP+YGFQWRHFGARYT+MHADYTGQGFDQLLDVINKIKNN
Sbjct: 361 NGSREYLDGIGLKDREEGDLGPIYGFQWRHFGARYTNMHADYTGQGFDQLLDVINKIKNN 420

Query: 421 PDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           PDDRRIILSAWNP DLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNI+SYA
Sbjct: 421 PDDRRIILSAWNPYDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNISSYA 480

Query: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFP-------------- 540
           LLTCMIAHVCGL  GDFIHV+GDAHVYR HVRPL+EQLQ+LPKPFP              
Sbjct: 481 LLTCMIAHVCGLVPGDFIHVLGDAHVYRNHVRPLKEQLQRLPKPFPLKFSLTSVYLILMQ 525

BLAST of Cla016577 vs. TrEMBL
Match: A0A0B2R0F7_GLYSO (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine soja GN=glysoja_010314 PE=3 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 4.1e-198
Identity = 340/435 (78.16%), Postives = 375/435 (86.21%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SA+ELLA+SPY
Sbjct: 70  NAVVMGRKTWESIPPENRPLPGRLNVVLTRSGSFDIATAENVLICGSMASAMELLASSPY 129

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
           CLSIEKVF+ GGGEI REALNAP C+A HITEIE ++ECDTFMP +D ++FQ WYSSFP+
Sbjct: 130 CLSIEKVFLTGGGEIFREALNAPGCEAAHITEIEASIECDTFMPRVDTNVFQTWYSSFPL 189

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
           VE++LRYSFT+Y RVRSS    +DQN     +   D   FE KKFSFLPK ++ERHEE+ 
Sbjct: 190 VEDNLRYSFTTYARVRSSPPESLDQNADPFFYNNSDSFKFEVKKFSFLPKMVYERHEEFK 249

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC-----------AG 356
           YL LVQEIIS+G  KDDRTGTGTLS+FGCQMR+NLR+ FPLLTTKA            A 
Sbjct: 250 YLRLVQEIISEGTTKDDRTGTGTLSKFGCQMRFNLRRNFPLLTTKALQEKGIHIWDGNAS 309

Query: 357 ILIGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRII 416
             IGL ERE+GDLGPVYGFQWRHFGA+YTDMHADY+GQGFDQLLDVINKIK+NP+DRRII
Sbjct: 310 RDIGLTEREKGDLGPVYGFQWRHFGAKYTDMHADYSGQGFDQLLDVINKIKHNPNDRRII 369

Query: 417 LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA 476
           LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA
Sbjct: 370 LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA 429

Query: 477 HVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFEL 536
           HVC L  GDFIHVIGDAHVYR HVRPLQEQLQ  PKPFPILKINP+KKDIDSFVA DF+L
Sbjct: 430 HVCDLVPGDFIHVIGDAHVYRNHVRPLQEQLQNQPKPFPILKINPKKKDIDSFVAADFKL 489

Query: 537 TGYDPHEKIAMKMAV 541
            GYDPH+KI MKMAV
Sbjct: 490 IGYDPHQKIEMKMAV 504

BLAST of Cla016577 vs. TrEMBL
Match: A0A0B2R0F7_GLYSO (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine soja GN=glysoja_010314 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 6.5e-42
Identity = 89/123 (72.36%), Postives = 102/123 (82.93%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPD--PRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNIT 60
           MA D L  SNGN N NA  +  P+RTYQVVV AT +MGI KD KLPW LP++L FF+ IT
Sbjct: 1   MATDALVISNGNGNGNAKSELHPQRTYQVVVVATRDMGISKDAKLPWTLPTELKFFEEIT 60

Query: 61  TTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSA 120
           TTTSD GK+NAV+MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SA
Sbjct: 61  TTTSDPGKKNAVVMGRKTWESIPPENRPLPGRLNVVLTRSGSFDIATAENVLICGSMASA 120

Query: 121 LEM 122
           +E+
Sbjct: 121 MEL 123

HSP 2 Score: 689.5 bits (1778), Expect = 3.3e-195
Identity = 354/561 (63.10%), Postives = 406/561 (72.37%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MA + L +    + +  P DP+RTYQVVVAAT+NMGIGKDGKLPWRLPSD+ FFK++T T
Sbjct: 1   MASEALANPTNGSGITRP-DPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD  KRNAV+MGRKTWESIP +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS+ SALE
Sbjct: 61  TSDPLKRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALE 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           +                         +GS    +VE V + G      E L         
Sbjct: 121 L------------------------LAGSPYCVSVEKVFVIGGGQIYREALN-------- 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
                            AP CDA+HITEIE ++ECDTF+P +D+S+FQPWYSSFP+VEN 
Sbjct: 181 -----------------APGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSFPLVENK 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +RY FT+YVRVR+S      Q NGL S  K D  NFE + FSFLPK +FE+HEEYLYL L
Sbjct: 241 IRYCFTTYVRVRNSVAELTSQTNGLSSDSKSDSGNFEIQNFSFLPKTVFEKHEEYLYLGL 300

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL---------- 360
           V+ IIS+G+ K+DRT TGT+S FGCQ    R  + +    ++    A IL          
Sbjct: 301 VENIISNGVTKNDRTRTGTVSIFGCQKVFWRGVVEELLWFISGSTNAKILKEKGVNIWEG 360

Query: 361 ---------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNN 420
                    IGL +REEGDLGP+YGFQWRHFGARYTDMHADY+GQGFDQLLDVI+KIKNN
Sbjct: 361 NGSREYLDSIGLTDREEGDLGPIYGFQWRHFGARYTDMHADYSGQGFDQLLDVISKIKNN 420

Query: 421 PDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           PDDRRIILSAWNPSDL+LMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 PDDRRIILSAWNPSDLRLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVC L  GDF+H IGDAHVY  HVRPL++QLQKLPKPFP+LKIN EK+DIDSF
Sbjct: 481 LLTCMIAHVCDLVPGDFVHSIGDAHVYCNHVRPLRDQLQKLPKPFPVLKINSEKRDIDSF 511

BLAST of Cla016577 vs. TrEMBL
Match: A0A0B0Q0A7_GOSAR (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Gossypium arboreum GN=F383_06128 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.3e-195
Identity = 365/553 (66.00%), Postives = 406/553 (73.42%), Query Frame = 1

Query: 10  NGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTTTSDHGKRNA 69
           NGN N NA PDP+RT+QVV                                T D G    
Sbjct: 11  NGNGNGNAQPDPQRTFQVV-----------------------------VAATKDMGVGKD 70

Query: 70  VLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALEMGRKTWESI 129
              G+  W+ +P++   L    ++ LT S S      +N +I         MGRKTWESI
Sbjct: 71  ---GKLPWK-LPSD---LKFFKDITLTTSDSGK----KNAVI---------MGRKTWESI 130

Query: 130 PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSIEKVFVIGGG 189
           P ++RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+TSALELLAASPYCLSIEKVFVIGGG
Sbjct: 131 PLKNRPLPGRLNVVLTRSGSFDIATAENVVICGSMTSALELLAASPYCLSIEKVFVIGGG 190

Query: 190 EILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENSLRYSFTSYV 249
           +I RE+LNA  CDAIHITEIETN+ECDTFMPAID ++FQPWYSSFP+VEN +RY FT+YV
Sbjct: 191 QIFRESLNAAGCDAIHITEIETNIECDTFMPAIDTAVFQPWYSSFPMVENDIRYCFTTYV 250

Query: 250 RVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNLVQEIISDGI 309
           RVR+ST   + QN  L   KK D   FE KKFSFLPK IF +HEEYLY+N++Q+IIS G 
Sbjct: 251 RVRTSTVEHLCQNCDLVVDKKSDTGKFEVKKFSFLPKMIFHKHEEYLYMNMIQDIISGGN 310

Query: 310 PKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL------------------- 369
            KDDRTGTGTLS+FGCQ    R  + +    ++    A +L                   
Sbjct: 311 LKDDRTGTGTLSKFGCQKVFWRGVVEELLWFISGSTNAKVLQEKGIHIWDGNASRDFLDS 370

Query: 370 IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILS 429
           IGL  REEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLD I+KIKNNP+DRRIILS
Sbjct: 371 IGLTSREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDAIDKIKNNPNDRRIILS 430

Query: 430 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 489
           AWNPSDLKLMALPPCHMFAQFYVAN ELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV
Sbjct: 431 AWNPSDLKLMALPPCHMFAQFYVANEELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 490

Query: 490 CGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTG 541
           C L  GDFIHV+GD HVY THVRPLQEQLQKLPKPFPILKINPEKK+IDSFVA DF+L G
Sbjct: 491 CDLIPGDFIHVLGDTHVYSTHVRPLQEQLQKLPKPFPILKINPEKKNIDSFVASDFKLIG 514

BLAST of Cla016577 vs. TrEMBL
Match: A0A0B2S695_GLYSO (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine soja GN=glysoja_020544 PE=3 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 4.7e-194
Identity = 339/457 (74.18%), Postives = 374/457 (81.84%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS++SA+ELLA+SPY
Sbjct: 68  NAVVMGRKTWESIPPENRPLPGRLNVVLTRSGSFDIATAENVLICGSVSSAMELLASSPY 127

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
           CLSIEKVF+ GGGEI REALNAP C+A HITEIE ++ECDTFMP ++ S+FQ WYSSFP+
Sbjct: 128 CLSIEKVFLTGGGEIFREALNAPGCEAAHITEIEASIECDTFMPRVNTSVFQTWYSSFPL 187

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
           VE++LRYSFT+YVRVRS      DQN         D   FE KKFSFLPK ++ERHEE+ 
Sbjct: 188 VEDNLRYSFTTYVRVRSFPPESPDQNADPFFDNNSDALKFEVKKFSFLPKMVYERHEEFK 247

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC--AGIL------- 356
           YL LVQEIIS+G  KDDRTGTGTLS+FGCQMR+NLR+ FPLLTTK     G++       
Sbjct: 248 YLRLVQEIISEGTTKDDRTGTGTLSKFGCQMRFNLRRNFPLLTTKKVFWRGVIEELLWFI 307

Query: 357 ------------------------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQ 416
                                   IGL +REEGDLGPVYGFQWRHFGA+YTDMHADY+GQ
Sbjct: 308 SGSTSAKVLQEKGIHIWDGNASRDIGLTDREEGDLGPVYGFQWRHFGAKYTDMHADYSGQ 367

Query: 417 GFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS 476
           GFDQLLDVINKIK+NPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS
Sbjct: 368 GFDQLLDVINKIKHNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS 427

Query: 477 ADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPF 536
           ADMGLGVPFNIASYALLTCMIAHVC L  GDFIHVIGDAHVYR HVRPLQEQLQ  PKPF
Sbjct: 428 ADMGLGVPFNIASYALLTCMIAHVCDLVPGDFIHVIGDAHVYRNHVRPLQEQLQNQPKPF 487

Query: 537 PILKINPEKKDIDSFVADDFELTGYDPHEKIAMKMAV 541
           PILKINP+KKDIDSFVA DF+L GY PH+KI MKMAV
Sbjct: 488 PILKINPKKKDIDSFVASDFKLIGYVPHQKIEMKMAV 524

BLAST of Cla016577 vs. NCBI nr
Match: gi|703133340|ref|XP_010105356.1| (Bifunctional dihydrofolate reductase-thymidylate synthase [Morus notabilis])

HSP 1 Score: 702.6 bits (1812), Expect = 5.4e-199
Identity = 367/576 (63.72%), Postives = 412/576 (71.53%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MAG+ + ++NGN NV   P  +RTYQVV AAT +MGIGKDGKLPWRLPSDL FFK IT T
Sbjct: 1   MAGENVITANGNGNVQTIP--QRTYQVVAAATRDMGIGKDGKLPWRLPSDLKFFKEITMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD GK+NAVLMGRKTWESIP EHRPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SALE
Sbjct: 61  TSDTGKKNAVLMGRKTWESIPLEHRPLPGRLNVVLTRSGSFDIATAENVLICGSMASALE 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           +                         + S    ++E V + G      E L AS      
Sbjct: 121 L------------------------LAASPYCLSIERVFVIGGGQILREALNASS----- 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
                               CDA+HITEIET++ECDTF+PAID S FQPWYSSFPVVEN+
Sbjct: 181 --------------------CDAVHITEIETSIECDTFIPAIDSSAFQPWYSSFPVVENN 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +R+SFT+YVRVRS      ++N  L    +PDFS FE KKF FLPK IF+RHEEY+YL L
Sbjct: 241 IRFSFTTYVRVRSPAIESPNENGDLAVDSEPDFSKFEIKKFFFLPKMIFDRHEEYVYLRL 300

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL---------- 360
           VQ+IISDGI KDDRTGTGT S+FGCQ    R  + +    ++    + +L          
Sbjct: 301 VQDIISDGISKDDRTGTGTFSKFGCQRLFWRGIVEELLWFISGSTSSKVLKDKGISIWDG 360

Query: 361 ---------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNN 420
                    IGL++REEGDLGP+YGFQWRHFGARYT+MHADYTGQGFDQLLDVINKIKNN
Sbjct: 361 NGSREYLDGIGLKDREEGDLGPIYGFQWRHFGARYTNMHADYTGQGFDQLLDVINKIKNN 420

Query: 421 PDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           PDDRRIILSAWNP DLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNI+SYA
Sbjct: 421 PDDRRIILSAWNPYDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNISSYA 480

Query: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFP-------------- 540
           LLTCMIAHVCGL  GDFIHV+GDAHVYR HVRPL+EQLQ+LPKPFP              
Sbjct: 481 LLTCMIAHVCGLVPGDFIHVLGDAHVYRNHVRPLKEQLQRLPKPFPLKFSLTSVYLILMQ 525

BLAST of Cla016577 vs. NCBI nr
Match: gi|734387634|gb|KHN25397.1| (Bifunctional dihydrofolate reductase-thymidylate synthase [Glycine soja])

HSP 1 Score: 699.1 bits (1803), Expect = 5.9e-198
Identity = 340/435 (78.16%), Postives = 375/435 (86.21%), Query Frame = 1

Query: 117 SALEMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPY 176
           +A+ MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SA+ELLA+SPY
Sbjct: 70  NAVVMGRKTWESIPPENRPLPGRLNVVLTRSGSFDIATAENVLICGSMASAMELLASSPY 129

Query: 177 CLSIEKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPV 236
           CLSIEKVF+ GGGEI REALNAP C+A HITEIE ++ECDTFMP +D ++FQ WYSSFP+
Sbjct: 130 CLSIEKVFLTGGGEIFREALNAPGCEAAHITEIEASIECDTFMPRVDTNVFQTWYSSFPL 189

Query: 237 VENSLRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYL 296
           VE++LRYSFT+Y RVRSS    +DQN     +   D   FE KKFSFLPK ++ERHEE+ 
Sbjct: 190 VEDNLRYSFTTYARVRSSPPESLDQNADPFFYNNSDSFKFEVKKFSFLPKMVYERHEEFK 249

Query: 297 YLNLVQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC-----------AG 356
           YL LVQEIIS+G  KDDRTGTGTLS+FGCQMR+NLR+ FPLLTTKA            A 
Sbjct: 250 YLRLVQEIISEGTTKDDRTGTGTLSKFGCQMRFNLRRNFPLLTTKALQEKGIHIWDGNAS 309

Query: 357 ILIGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRII 416
             IGL ERE+GDLGPVYGFQWRHFGA+YTDMHADY+GQGFDQLLDVINKIK+NP+DRRII
Sbjct: 310 RDIGLTEREKGDLGPVYGFQWRHFGAKYTDMHADYSGQGFDQLLDVINKIKHNPNDRRII 369

Query: 417 LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA 476
           LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA
Sbjct: 370 LSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIA 429

Query: 477 HVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFEL 536
           HVC L  GDFIHVIGDAHVYR HVRPLQEQLQ  PKPFPILKINP+KKDIDSFVA DF+L
Sbjct: 430 HVCDLVPGDFIHVIGDAHVYRNHVRPLQEQLQNQPKPFPILKINPKKKDIDSFVAADFKL 489

Query: 537 TGYDPHEKIAMKMAV 541
            GYDPH+KI MKMAV
Sbjct: 490 IGYDPHQKIEMKMAV 504

BLAST of Cla016577 vs. NCBI nr
Match: gi|734387634|gb|KHN25397.1| (Bifunctional dihydrofolate reductase-thymidylate synthase [Glycine soja])

HSP 1 Score: 180.3 bits (456), Expect = 9.3e-42
Identity = 89/123 (72.36%), Postives = 102/123 (82.93%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPD--PRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNIT 60
           MA D L  SNGN N NA  +  P+RTYQVVV AT +MGI KD KLPW LP++L FF+ IT
Sbjct: 1   MATDALVISNGNGNGNAKSELHPQRTYQVVVVATRDMGISKDAKLPWTLPTELKFFEEIT 60

Query: 61  TTTSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSA 120
           TTTSD GK+NAV+MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SA
Sbjct: 61  TTTSDPGKKNAVVMGRKTWESIPPENRPLPGRLNVVLTRSGSFDIATAENVLICGSMASA 120

Query: 121 LEM 122
           +E+
Sbjct: 121 MEL 123

HSP 2 Score: 697.2 bits (1798), Expect = 2.3e-197
Identity = 370/553 (66.91%), Postives = 410/553 (74.14%), Query Frame = 1

Query: 10  NGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTTTSDHGKRNA 69
           NGN N NA PDP+RTYQVVVA                   D+   K+             
Sbjct: 11  NGNGNGNAQPDPQRTYQVVVA----------------ATKDMGIGKD------------- 70

Query: 70  VLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALEMGRKTWESI 129
              G+  W ++P++   L    ++ LT S S      +N +I         MGRKTWESI
Sbjct: 71  ---GKLPW-NLPSD---LKFFKDITLTTSDSGK----KNAVI---------MGRKTWESI 130

Query: 130 PAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSIEKVFVIGGG 189
           P ++RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+TSALELLAASPYCLSIEKVFVIGGG
Sbjct: 131 PLKNRPLPGRLNVVLTRSGSFDIATAENVVICGSMTSALELLAASPYCLSIEKVFVIGGG 190

Query: 190 EILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENSLRYSFTSYV 249
           +ILRE+LNA  CDAIHITEIETN+ECDTFMPAID ++FQPWYSSFP+VEN +RY FT+YV
Sbjct: 191 QILRESLNAAGCDAIHITEIETNIECDTFMPAIDTAVFQPWYSSFPMVENDIRYCFTTYV 250

Query: 250 RVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNLVQEIISDGI 309
           RVR+ST   + QN  L    KPD   FE KKFSFLPK IF +HEEYLYLN++Q+IIS G 
Sbjct: 251 RVRTSTIEHLCQNCDLVVDNKPDTGKFEVKKFSFLPKMIFRKHEEYLYLNMIQDIISGGN 310

Query: 310 PKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL------------------- 369
            KDDRTGTGTLS FGCQ    R  + +    ++    A +L                   
Sbjct: 311 LKDDRTGTGTLSMFGCQKVFWRGVVEELLWFISGSTNAEVLQEKGIHIWDGNASRDFLDS 370

Query: 370 IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILS 429
           IGL  REEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVI+KIKNNP+DRRIILS
Sbjct: 371 IGLTSREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVIDKIKNNPNDRRIILS 430

Query: 430 AWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 489
           AWNPSDLKLMALPPCHMFAQFYVAN ELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV
Sbjct: 431 AWNPSDLKLMALPPCHMFAQFYVANEELSCQMYQRSADMGLGVPFNIASYALLTCMIAHV 490

Query: 490 CGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTG 541
           C L  GDFIHV+GD HVY THVRPLQEQLQKLPKPFPILKINPEKK+IDSFVA DF+L G
Sbjct: 491 CDLIPGDFIHVLGDTHVYSTHVRPLQEQLQKLPKPFPILKINPEKKNIDSFVASDFKLIG 514

BLAST of Cla016577 vs. NCBI nr
Match: gi|697116519|ref|XP_009612175.1| (PREDICTED: bifunctional dihydrofolate reductase-thymidylate synthase-like [Nicotiana tomentosiformis])

HSP 1 Score: 693.7 bits (1789), Expect = 2.5e-196
Identity = 363/577 (62.91%), Postives = 414/577 (71.75%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MA + +T  + N ++NA   P+RTYQVVVA                            T 
Sbjct: 1   MASETITGLS-NGSINAHSTPQRTYQVVVA---------------------------ATR 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
               GK      G+  W         LP  L      +G+    + +N +I         
Sbjct: 61  NMGIGKE-----GKLPWR--------LPSDLKFFKGITGTTSDPSRKNAVI--------- 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           MGRKTWESIP E+RPLPGRLNVVLTRSGSFDIAT ENV+ICGS+ SAL+LLAASPY LSI
Sbjct: 121 MGRKTWESIPLEYRPLPGRLNVVLTRSGSFDIATAENVVICGSLGSALQLLAASPYGLSI 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
           E VFVIGGGEI R++LNA  CDAIHITEIET++ECDTF+PAI+ S+FQPWYSSFPVVEN 
Sbjct: 181 ENVFVIGGGEIFRDSLNAHGCDAIHITEIETDIECDTFIPAIETSVFQPWYSSFPVVENK 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +RYSFT+YVRV++S    ++Q N        D  N E K FSFLPK IFE+HEEY+YL L
Sbjct: 241 IRYSFTTYVRVKNSEVETVNQANNETPEIGSDSFNVEVKAFSFLPKMIFEKHEEYMYLRL 300

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTKAC--AGIL----------- 360
           ++EIIS+G+PKDDRTGTGTLS+FGCQMR+NLRK+FPLLTTK     G+L           
Sbjct: 301 IEEIISNGMPKDDRTGTGTLSKFGCQMRFNLRKSFPLLTTKKVFWRGVLEELLWFISGST 360

Query: 361 ------------------------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQ 420
                                   IGL++REEGDLGPVYGFQWRHFGARY DMH DY+GQ
Sbjct: 361 SAKVLQEKGIHIWDGNASREYLDSIGLKDREEGDLGPVYGFQWRHFGARYIDMHTDYSGQ 420

Query: 421 GFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS 480
           GFDQL DVINK+KNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS
Sbjct: 421 GFDQLADVINKVKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRS 480

Query: 481 ADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPF 540
           ADMGLGVPFNIASYALLTCMIAHV  L  GDF+HVIGDAHVYRTHVRPLQ+QLQKLP+PF
Sbjct: 481 ADMGLGVPFNIASYALLTCMIAHVSDLVPGDFVHVIGDAHVYRTHVRPLQDQLQKLPRPF 527

BLAST of Cla016577 vs. NCBI nr
Match: gi|1021053226|gb|KZN11004.1| (hypothetical protein DCAR_003660 [Daucus carota subsp. sativus])

HSP 1 Score: 689.5 bits (1778), Expect = 4.7e-195
Identity = 354/561 (63.10%), Postives = 406/561 (72.37%), Query Frame = 1

Query: 1   MAGDPLTSSNGNANVNAPPDPRRTYQVVVAATENMGIGKDGKLPWRLPSDLSFFKNITTT 60
           MA + L +    + +  P DP+RTYQVVVAAT+NMGIGKDGKLPWRLPSD+ FFK++T T
Sbjct: 1   MASEALANPTNGSGITRP-DPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMT 60

Query: 61  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120
           TSD  KRNAV+MGRKTWESIP +HRPLPGRLNVVLTRSGSFDIATVENV+ICGS+ SALE
Sbjct: 61  TSDPLKRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALE 120

Query: 121 MGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALELLAASPYCLSI 180
           +                         +GS    +VE V + G      E L         
Sbjct: 121 L------------------------LAGSPYCVSVEKVFVIGGGQIYREALN-------- 180

Query: 181 EKVFVIGGGEILREALNAPECDAIHITEIETNVECDTFMPAIDKSMFQPWYSSFPVVENS 240
                            AP CDA+HITEIE ++ECDTF+P +D+S+FQPWYSSFP+VEN 
Sbjct: 181 -----------------APGCDAVHITEIEEHIECDTFIPLLDESVFQPWYSSFPLVENK 240

Query: 241 LRYSFTSYVRVRSSTESPIDQNNGLDSHKKPDFSNFEAKKFSFLPKEIFERHEEYLYLNL 300
           +RY FT+YVRVR+S      Q NGL S  K D  NFE + FSFLPK +FE+HEEYLYL L
Sbjct: 241 IRYCFTTYVRVRNSVAELTSQTNGLSSDSKSDSGNFEIQNFSFLPKTVFEKHEEYLYLGL 300

Query: 301 VQEIISDGIPKDDRTGTGTLSRFGCQ---MRYNLRKTFPLLTTKACAGIL---------- 360
           V+ IIS+G+ K+DRT TGT+S FGCQ    R  + +    ++    A IL          
Sbjct: 301 VENIISNGVTKNDRTRTGTVSIFGCQKVFWRGVVEELLWFISGSTNAKILKEKGVNIWEG 360

Query: 361 ---------IGLREREEGDLGPVYGFQWRHFGARYTDMHADYTGQGFDQLLDVINKIKNN 420
                    IGL +REEGDLGP+YGFQWRHFGARYTDMHADY+GQGFDQLLDVI+KIKNN
Sbjct: 361 NGSREYLDSIGLTDREEGDLGPIYGFQWRHFGARYTDMHADYSGQGFDQLLDVISKIKNN 420

Query: 421 PDDRRIILSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480
           PDDRRIILSAWNPSDL+LMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA
Sbjct: 421 PDDRRIILSAWNPSDLRLMALPPCHMFAQFYVANGELSCQMYQRSADMGLGVPFNIASYA 480

Query: 481 LLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPILKINPEKKDIDSF 540
           LLTCMIAHVC L  GDF+H IGDAHVY  HVRPL++QLQKLPKPFP+LKIN EK+DIDSF
Sbjct: 481 LLTCMIAHVCDLVPGDFVHSIGDAHVYCNHVRPLRDQLQKLPKPFPVLKINSEKRDIDSF 511