Cla015663 (gene) Watermelon (97103) v1

NameCla015663
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPolygalacturonase (AHRD V1 ***- E3VSV7_CUCPE); contains Interpro domain(s) IPR000743 Glycoside hydrolase, family 28
LocationChr2 : 2448387 .. 2449614 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTGATGAAATCAAGTTTTCTGGTTACTGCATTCTTGATGTCCTTGTTATCATTTATTGTTGAGTCTCGATCTCATGTTGATGTTATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCCGATGCTGATGTCACTGAGGTAAACACACACAATAAAGTATATATCAAAGTGATTACTTATTGAATTGATCAATTGTTTTTGCTAATTAACTTTTGTTATCTGTGAAACATAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAGTGATTATTCCAAAGGGGATATTTAAAGTAAGCGAAGTAAAATTTAAAGGCCCTTGCAAGAGCCCTATTGAAATTCGACTTCTAGGAACATTGCAGGCTCCAAAACACCCTCATGGAGATAGGTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGCGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGTATGGGAAAAAAATGATTGCCATATAAACATAAATTGTGTTGACCTCCCTATCGTAAGTTCTATACCTATTGTTTTCCTTTCCAATCTCATTCGTCTTTAATTAATTTGTTGATTTTCAAGGAATTTGTTAGATGTCCTTGTCTACTATTTCATTTTTACAACTTGGTAAATGTTATTAATATTGTATAGGATAATGCTTAAGGAATTAAATTTGGAACTAAATTTAACCGTTAGAAAAAAACTAGGTAAGAGAGCTATAATTTCAAAATATAAAGATTATATAAAGGAAAAACTCATCATTTTGGTATCTCCTTACAAAAATTTGTGGCTCTCATCTTAGCTCTAACATGACTTATTGATCATATAAAATGAACAGAGTTTAAGGTTCGATTTCATTACCAATTCAATTGTGAAGAGAATAACATCACTCAATAGCAAGAACTTCCATGTCAACATCCTTGGTTGCAACAACCTCACATTCCATGGTGTCAACATAATTGCACCTGAAAATAGTCCCAACACGGATGGAATACACATTGGCCGATCAATTGGGATCTCAATCACAAAGTCTCGAATAGCAACAGGAGATGATTGCATCTCCCTCGGAGATGGAAGCAAACGAGTAAAAGTCACGAACGTGACATGTGGACCAGGTCATGGAATAAGCATTGGAAGTCTTGGAAAATACACCAATGAAGAACCTGTTGAAGGTGTCATAGTAAAGAACTGCACTGCACCATAA

mRNA sequence

ATGGGTGTGATGAAATCAAGTTTTCTGGTTACTGCATTCTTGATGTCCTTGTTATCATTTATTGTTGAGTCTCGATCTCATGTTGATGTTATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCCGATGCTGATGTCACTGAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAGTGATTATTCCAAAGGGGATATTTAAAGTAAGCGAAGTAAAATTTAAAGGCCCTTGCAAGAGCCCTATTGAAATTCGACTTCTAGGAACATTGCAGGCTCCAAAACACCCTCATGGAGATAGGTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGCGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGTATGGGAAAAAAATGATTGCCATATAAACATAAATTGTGTTGACCTCCCTATCAGTTTAAGGTTCGATTTCATTACCAATTCAATTGTGAAGAGAATAACATCACTCAATAGCAAGAACTTCCATGTCAACATCCTTGGTTGCAACAACCTCACATTCCATGGTGTCAACATAATTGCACCTGAAAATAGTCCCAACACGGATGGAATACACATTGGCCGATCAATTGGGATCTCAATCACAAAGTCTCGAATAGCAACAGGAGATGATTGCATCTCCCTCGGAGATGGAAGCAAACGAGTAAAAGTCACGAACGTGACATGTGGACCAGGTCATGGAATAAGCATTGGAAGTCTTGGAAAATACACCAATGAAGAACCTGTTGAAGGTGTCATAGTAAAGAACTGCACTGCACCATAA

Coding sequence (CDS)

ATGGGTGTGATGAAATCAAGTTTTCTGGTTACTGCATTCTTGATGTCCTTGTTATCATTTATTGTTGAGTCTCGATCTCATGTTGATGTTATTTTTCCTTTGAGGAAGTATGGTGACATTGTGCCCGATGCTGATGTCACTGAGGCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGTTCGTCCAAGTGCAGTGATTATTCCAAAGGGGATATTTAAAGTAAGCGAAGTAAAATTTAAAGGCCCTTGCAAGAGCCCTATTGAAATTCGACTTCTAGGAACATTGCAGGCTCCAAAACACCCTCATGGAGATAGGTGGATTACTTTTGCATATGTTGATAGATTGACACTATCAGGCGGAGGAGTTTTTGATGGTCAAGGAAAGGCAGTATGGGAAAAAAATGATTGCCATATAAACATAAATTGTGTTGACCTCCCTATCAGTTTAAGGTTCGATTTCATTACCAATTCAATTGTGAAGAGAATAACATCACTCAATAGCAAGAACTTCCATGTCAACATCCTTGGTTGCAACAACCTCACATTCCATGGTGTCAACATAATTGCACCTGAAAATAGTCCCAACACGGATGGAATACACATTGGCCGATCAATTGGGATCTCAATCACAAAGTCTCGAATAGCAACAGGAGATGATTGCATCTCCCTCGGAGATGGAAGCAAACGAGTAAAAGTCACGAACGTGACATGTGGACCAGGTCATGGAATAAGCATTGGAAGTCTTGGAAAATACACCAATGAAGAACCTGTTGAAGGTGTCATAGTAAAGAACTGCACTGCACCATAA

Protein sequence

MGVMKSSFLVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLSGGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTAP
BLAST of Cla015663 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.5e-72
Identity = 131/227 (57.71%), Postives = 162/227 (71.37%), Query Frame = 1

Query: 45  DVTEALKDAWNDACASVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPH- 104
           D T+AL  AW +ACAS  PS +++PKG F V  +  +GPCKS I ++L GTL+AP  P  
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 105 --GDRWITFAYVDRLTLSGGGVFDGQGKAVWEKNDCHININ-CVDLPISLRFDFITNSIV 164
             G  WI    +D LT+ GGGVFDGQGK+ W +NDCH N   C  L ++LR   +TNSI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 165 KRITSLNSKNFHVNILGCNNLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDD 224
           + +T+L+SKNFHVN++GC NLTF    I A E S NTDGIHIGRS G++I  + I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 225 CISLGDGSKRVKVTNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           CISLGDGSK + +TN+TCGPGHGIS+GSLG+Y NEE V G+ VKNCT
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCT 227

BLAST of Cla015663 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 9.5e-72
Identity = 128/263 (48.67%), Postives = 177/263 (67.30%), Query Frame = 1

Query: 9   LVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPD-ADVTEALKDAWNDACASVRPSAVI 68
           +V +  + LL FI  S+   D    + K+G       D+++   DAW +ACASV PS V+
Sbjct: 7   IVPSMFVLLLLFISASKVQPDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69  IPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDR---WITFAYVDRLTLSGGGVF 128
           IPKG + +S+V  +GPCK+PIEI + GT+QAP  P   +   W+ F  V+   + GGG+F
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIF 126

Query: 129 DGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFH 188
           DGQG   +EKN C        LP+++RFDF+TN++++ ITS +SK FH+N+  C N+T  
Sbjct: 127 DGQGSIAYEKNTCENREFRSKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLE 186

Query: 189 GVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGI 248
            + I AP+ SPNTDGIH+G+S G++I  S I TGDDCIS+GDG+K + +  +TCGPGHGI
Sbjct: 187 RLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGI 246

Query: 249 SIGSLGKYTNEEPVEGVIVKNCT 268
           SIGSLGK+ NEEPVEG+ + NCT
Sbjct: 247 SIGSLGKFQNEEPVEGIKISNCT 269

BLAST of Cla015663 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-71
Identity = 128/263 (48.67%), Postives = 177/263 (67.30%), Query Frame = 1

Query: 9   LVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPD-ADVTEALKDAWNDACASVRPSAVI 68
           +V +  + LL FI  S+   D    + K+G       D+++   DAW +ACASV PS V+
Sbjct: 7   IVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVV 66

Query: 69  IPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDR---WITFAYVDRLTLSGGGVF 128
           IPKG + +S+V  +GPCK+PIEI + GT+QAP  P   +   W+ F  V+   + GGG+F
Sbjct: 67  IPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIF 126

Query: 129 DGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFH 188
           DGQG   +EKN C        LP+++RFDF+TN++++ ITS +SK FH+N+  C N+T  
Sbjct: 127 DGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLE 186

Query: 189 GVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGI 248
            + I AP+ SPNTDGIH+G+S G++I  S I TGDDCIS+GDG+K + +  +TCGPGHGI
Sbjct: 187 RLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGI 246

Query: 249 SIGSLGKYTNEEPVEGVIVKNCT 268
           SIGSLGK+ NEEPVEG+ + NCT
Sbjct: 247 SIGSLGKFQNEEPVEGIKISNCT 269

BLAST of Cla015663 vs. Swiss-Prot
Match: PGLR_MEDSA (Polygalacturonase OS=Medicago sativa PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.9e-67
Identity = 127/266 (47.74%), Postives = 179/266 (67.29%), Query Frame = 1

Query: 11  TAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVIIPK 70
           TA ++S L FI +  +    +  + K+G   P++D+ +AL  AWN+ACAS   + ++IP 
Sbjct: 5   TAIIVSFL-FIADFCAAQSGVLDISKFGG-KPNSDIGQALTSAWNEACASTTAAKIVIPA 64

Query: 71  GIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPH----GDRWITFAYVDRLTLSGGGVFDG 130
           G ++++ ++ KGPCK+PIE+++ GT+QAP  P      ++W  F Y+D LTLSG GVFDG
Sbjct: 65  GTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDG 124

Query: 131 QGKAVWEKND-----CHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNL 190
           QG  V++K          N N   + ++  F+F+ NSIV+ +TS +SKNFHV + GC N+
Sbjct: 125 QGATVYKKAAPASAWSGKNSNS-KVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNI 184

Query: 191 TFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPG 250
           TF G  I AP +SPNTDGIH+G+S  + I  + I TGDDC+S+GDGSK++ V  V CGPG
Sbjct: 185 TFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPG 244

Query: 251 HGISIGSLGKYTNEEPVEGVIVKNCT 268
           HG+S+GSLGK+T EE VEG+ VKNCT
Sbjct: 245 HGLSVGSLGKFTTEENVEGITVKNCT 267

BLAST of Cla015663 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.2e-67
Identity = 127/259 (49.03%), Postives = 177/259 (68.34%), Query Frame = 1

Query: 12  AFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVIIPKG 71
           A ++  L+   ES++ V   F + KYG    +AD++EAL +A+ +AC S  PS ++IPKG
Sbjct: 10  ALVLLFLAHFGESQTGV---FDITKYG-ANSNADISEALLNAFKEACQSTSPSTIVIPKG 69

Query: 72  IFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHG---DRWITFAYVDRLTLSGGGVFDGQG 131
            F +++VK +GPCKSP+E+++  TL+AP  P       W+T   +D+ T+SGGG+ DGQ 
Sbjct: 70  TFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQA 129

Query: 132 KAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFHGVNI 191
            A WE   C  +  C  LP +L F+ +TNS +K IT+L+SK+FHVN+  C NLTF   N+
Sbjct: 130 AAAWE---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNV 189

Query: 192 IAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGISIGS 251
            AP NSPNTDGIH+ RS  ++IT S  +TGDDCIS+GD ++++ +T VTCGPGHGIS+GS
Sbjct: 190 SAPANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGS 249

Query: 252 LGKYTNEEPVEGVIVKNCT 268
           LG   +E+PV GV V+NCT
Sbjct: 250 LGGNPDEKPVVGVFVRNCT 261

BLAST of Cla015663 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 6.1e-126
Identity = 227/268 (84.70%), Postives = 239/268 (89.18%), Query Frame = 1

Query: 1   MGVMKSSFL-VTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACA 60
           MGV+K   L VT FLMSLLSF +E+RSHVD IF LRKYG+IV  ADVTEALKDAWNDACA
Sbjct: 1   MGVIKHLILLVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61  SVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLS 120
           S RPSAV+IPKG FKV E +FKGPCKSPIE R+ GTLQAPKHPHGD WITFAYVDR+ LS
Sbjct: 61  SDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLS 120

Query: 121 GGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCN 180
           GGGVFDGQGKA WEKNDCH  I+C  LPISLRFDFITNSIV+RITSL+SKNFHVNILGCN
Sbjct: 121 GGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCN 180

Query: 181 NLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCG 240
           NLTF GVNIIAPENSPNTDGIHIGRSIGISI KSRIATGDDCISLGDGSKR+KV NV CG
Sbjct: 181 NLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICG 240

Query: 241 PGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           PGHGISIGSLGKY NEEPVEGVIV NCT
Sbjct: 241 PGHGISIGSLGKYNNEEPVEGVIVNNCT 267

BLAST of Cla015663 vs. TrEMBL
Match: W9SC16_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020041 PE=3 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.2e-83
Identity = 155/271 (57.20%), Postives = 198/271 (73.06%), Query Frame = 1

Query: 1   MGVMKSSFLVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACAS 60
           MG+  S+F++ A L+ + S I  ++    V+F + + G I+P +D+T+AL  AWN+ACAS
Sbjct: 1   MGLKLSNFVIAALLLLVSSNIANAQI---VLFDVTENGTILPQSDITDALLKAWNNACAS 60

Query: 61  VRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPH----GDRWITFAYVDRL 120
             PS V++PKGIFK+S    +GPC +PIE  L G LQAP        GD W+ F YVD L
Sbjct: 61  TIPSKVVVPKGIFKLSGATLEGPCNAPIEFDLQGILQAPPEDGNFTGGDTWVAFEYVDLL 120

Query: 121 TLSGGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNIL 180
           T+SGGG+FDGQGK  W K DC  +  C DLPI++RF+++TNSI++ ITSL+SK FH+N+L
Sbjct: 121 TISGGGIFDGQGKPTWGK-DCANDEYCGDLPINIRFNYVTNSIIQEITSLDSKQFHINVL 180

Query: 181 GCNNLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNV 240
           GC N+TF  V IIA ENSP+TDGIHIGRS  I +  S I TGDDCISLGDGSK++ +  V
Sbjct: 181 GCQNVTFQHVKIIAAENSPHTDGIHIGRSTDIKVLDSNIQTGDDCISLGDGSKQITIAKV 240

Query: 241 TCGPGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           TCGPGHGISIGSLGKY NEE V+GV VK+CT
Sbjct: 241 TCGPGHGISIGSLGKYLNEEAVKGVDVKSCT 267

BLAST of Cla015663 vs. TrEMBL
Match: W9SBW1_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020040 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.9e-80
Identity = 150/263 (57.03%), Postives = 192/263 (73.00%), Query Frame = 1

Query: 9   LVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVII 68
           ++ A+ +SLL  +  +R    ++F +  YG  + + D+T+AL +AW DACAS     V+I
Sbjct: 7   IIGAYFLSLLLALASARK--PLVFDVTTYGAQL-NMDITQALSNAWKDACASPIRGKVLI 66

Query: 69  PKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHP----HGDRWITFAYVDRLTLSGGGVF 128
           P+G +K+S    +GPCK PI+++L GTL+AP  P     GD W+TF  VD LTL+GGG F
Sbjct: 67  PRGTYKLSRATLEGPCKVPIKVQLQGTLRAPTDPSGFNEGDGWVTFRNVDGLTLAGGGTF 126

Query: 129 DGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFH 188
           DGQG AVW KN C     C  LPI+ RFDF+TNS+++ I SLNSK FH+N+LG  N+TF 
Sbjct: 127 DGQGSAVWGKN-CEQQQYCGKLPINFRFDFVTNSLIRDIISLNSKQFHINVLGGQNVTFS 186

Query: 189 GVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGI 248
            V IIAPE+SPNTDGIHIGRS GISIT + I TGDDC+SLGDG K + +  VTCGPGHGI
Sbjct: 187 RVKIIAPEDSPNTDGIHIGRSSGISITDTVIQTGDDCVSLGDGCKEISIRRVTCGPGHGI 246

Query: 249 SIGSLGKYTNEEPVEGVIVKNCT 268
           S+GSLGKY+NE+PVEGVIV+NCT
Sbjct: 247 SVGSLGKYSNEDPVEGVIVRNCT 265

BLAST of Cla015663 vs. TrEMBL
Match: A0A147BN67_IXORI (Putative polygalacturonase/glycoside hydrolase family protein (Fragment) OS=Ixodes ricinus PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.1e-79
Identity = 142/241 (58.92%), Postives = 179/241 (74.27%), Query Frame = 1

Query: 31  IFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVIIPKGIFKVSEVKFKGPCKSPIEI 90
           +F ++ YG   P+AD+T+AL +AW DACA++  S V++P G +K+  V  KGPCK PIE+
Sbjct: 2   VFDVKTYG-AKPNADITQALSNAWKDACAAIGSSKVVVPAGTYKLGLVSLKGPCKGPIEV 61

Query: 91  RLLGTLQAPKHPH----GDRWITFAYVDRLTLSGGGVFDGQGKAVWEKNDCHININCVDL 150
           ++ GTLQAP  P      D W+ F  +D+ TLSG G FDGQG+  W++NDC    NC  L
Sbjct: 62  QVEGTLQAPVDPGYFKGSDGWVVFERIDQFTLSGSGSFDGQGQMAWKQNDCSKTFNCKTL 121

Query: 151 PISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFHGVNIIAPENSPNTDGIHIGRSI 210
            +++RF+FITNS+V+ I SLNSKNFH+N+LGC N TF  V I AP +SPNTDGIHIGRS 
Sbjct: 122 AVNIRFNFITNSLVRDIKSLNSKNFHINLLGCKNFTFQHVTITAPGDSPNTDGIHIGRSS 181

Query: 211 GISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNC 268
           GI+IT + IATGDDCIS+GDGS+ V +  VTCGPGHGISIGSLGKYTNEEPV G+ V  C
Sbjct: 182 GINITDTNIATGDDCISMGDGSQDVTINKVTCGPGHGISIGSLGKYTNEEPVSGIKVIGC 241

BLAST of Cla015663 vs. TrEMBL
Match: Q9MBB9_SALGI (Polygalacturonase OS=Salix gilgiana GN=SgPG2 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 2.8e-78
Identity = 143/265 (53.96%), Postives = 188/265 (70.94%), Query Frame = 1

Query: 6   SSFLVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSA 65
           SS +++  L+ LL+   +++S+   +F + KYG      D+TEAL +AW DACAS  PS 
Sbjct: 7   SSAIISFSLLLLLASTAKAQSNG--VFDVTKYGG---KEDITEALNNAWKDACASTNPSK 66

Query: 66  VIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPH---GDRWITFAYVDRLTLSGGG 125
           V+IP G + + +V   GPCK+ IE+++ G L+AP +P    G  W+ F Y+D+LTLSG G
Sbjct: 67  VLIPSGTYSLRQVTLAGPCKAAIELQVNGILKAPVNPDQFSGSHWVNFRYIDQLTLSGSG 126

Query: 126 VFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLT 185
            FDGQG   W K+ C  N NC  LP+++RFDFITN +V+ IT+ +SKNFHVN+LGC NLT
Sbjct: 127 TFDGQGNVAWSKSTCSKNKNCEGLPMNIRFDFITNGLVRDITTRDSKNFHVNVLGCKNLT 186

Query: 186 FHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGH 245
           F    + AP  S NTDGIHIGRS GI I  S+I TGDDCIS+GDG++ + VT VTCGPGH
Sbjct: 187 FQHFTVTAPAESINTDGIHIGRSTGIYIIDSKIGTGDDCISVGDGTEELHVTGVTCGPGH 246

Query: 246 GISIGSLGKYTNEEPVEGVIVKNCT 268
           GIS+GSLG+Y NE+PV G+ VKNCT
Sbjct: 247 GISVGSLGRYPNEKPVSGIFVKNCT 266

BLAST of Cla015663 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 473.0 bits (1216), Expect = 3.4e-130
Identity = 231/268 (86.19%), Postives = 248/268 (92.54%), Query Frame = 1

Query: 1   MGVMK-SSFLVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACA 60
           M V+K SS LV  FLM+LLSF VESRSHVDVIF LRKYG+IV  ADVTEALKDAWNDACA
Sbjct: 1   MSVIKYSSLLVITFLMALLSFTVESRSHVDVIFHLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61  SVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLS 120
           S+RPSAV+IPKG FKV E +FKGPCKSP+E+R+ GTLQAPKHPHGD WITFAYVDR+TLS
Sbjct: 61  SLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWITFAYVDRMTLS 120

Query: 121 GGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCN 180
           GGGVFDGQGKA WEKNDCH +I+C  LPISLRF+FITNSIV+RITSL+SKNFHVNILGCN
Sbjct: 121 GGGVFDGQGKAGWEKNDCHKHISCARLPISLRFNFITNSIVRRITSLDSKNFHVNILGCN 180

Query: 181 NLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCG 240
           NLTF GVNIIAPE+SPNTDGIHIGRSIGISI KSRIATGDDCISLGDGSKRVK+TNVTCG
Sbjct: 181 NLTFDGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRVKITNVTCG 240

Query: 241 PGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           PGHGISIGSLGKYTNEEPVEGVIVKNCT
Sbjct: 241 PGHGISIGSLGKYTNEEPVEGVIVKNCT 268

BLAST of Cla015663 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 458.4 bits (1178), Expect = 8.8e-126
Identity = 227/268 (84.70%), Postives = 239/268 (89.18%), Query Frame = 1

Query: 1   MGVMKSSFL-VTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACA 60
           MGV+K   L VT FLMSLLSF +E+RSHVD IF LRKYG+IV  ADVTEALKDAWNDACA
Sbjct: 1   MGVIKHLILLVTTFLMSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACA 60

Query: 61  SVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLS 120
           S RPSAV+IPKG FKV E +FKGPCKSPIE R+ GTLQAPKHPHGD WITFAYVDR+ LS
Sbjct: 61  SDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLS 120

Query: 121 GGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCN 180
           GGGVFDGQGKA WEKNDCH  I+C  LPISLRFDFITNSIV+RITSL+SKNFHVNILGCN
Sbjct: 121 GGGVFDGQGKAGWEKNDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCN 180

Query: 181 NLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCG 240
           NLTF GVNIIAPENSPNTDGIHIGRSIGISI KSRIATGDDCISLGDGSKR+KV NV CG
Sbjct: 181 NLTFQGVNIIAPENSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICG 240

Query: 241 PGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           PGHGISIGSLGKY NEEPVEGVIV NCT
Sbjct: 241 PGHGISIGSLGKYNNEEPVEGVIVNNCT 267

BLAST of Cla015663 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 447.2 bits (1149), Expect = 2.0e-122
Identity = 218/253 (86.17%), Postives = 229/253 (90.51%), Query Frame = 1

Query: 15  MSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACASVRPSAVIIPKGIFK 74
           MSLLSF +E+RSHVD IF LRKYG+IV  ADVTEALKDAWNDACAS RPSAV+IPKG FK
Sbjct: 1   MSLLSFTIEARSHVD-IFSLRKYGNIVSGADVTEALKDAWNDACASDRPSAVVIPKGTFK 60

Query: 75  VSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLSGGGVFDGQGKAVWEK 134
           V E +FKGPCKSPIE R+ GTLQAPKHPHGD WITFAYVDR+ LSGGGVFDGQGKA WEK
Sbjct: 61  VREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRMRLSGGGVFDGQGKAGWEK 120

Query: 135 NDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCNNLTFHGVNIIAPENS 194
           NDCH  I+C  LPISLRFDFITNSIV+RITSL+SKNFHVNILGCNNLTF GVNIIAPENS
Sbjct: 121 NDCHKRIDCAKLPISLRFDFITNSIVRRITSLDSKNFHVNILGCNNLTFQGVNIIAPENS 180

Query: 195 PNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCGPGHGISIGSLGKYTN 254
           PNTDGIHIGRSIGISI KSRIATGDDCISLGDGSKR+KV NV CGPGHGISIGSLGKY N
Sbjct: 181 PNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNN 240

Query: 255 EEPVEGVIVKNCT 268
           EEPVEGVIV NCT
Sbjct: 241 EEPVEGVIVNNCT 252

BLAST of Cla015663 vs. NCBI nr
Match: gi|778730075|ref|XP_011659692.1| (PREDICTED: polygalacturonase-like [Cucumis sativus])

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-121
Identity = 221/268 (82.46%), Postives = 239/268 (89.18%), Query Frame = 1

Query: 1   MGVMKSSFL-VTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACA 60
           MG++K S L +T FLMSLLSF +ES+SHVDVIFPLRKYG+I        ALKDAWNDACA
Sbjct: 1   MGMIKYSNLSITTFLMSLLSFTIESQSHVDVIFPLRKYGNI--------ALKDAWNDACA 60

Query: 61  SVRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPHGDRWITFAYVDRLTLS 120
           SVRPSAV+I  GIFKV E +FKGPCKSPIEIR+ GTLQAPKHPHGD WITFAYVDR+T+S
Sbjct: 61  SVRPSAVVIRDGIFKVREGEFKGPCKSPIEIRVRGTLQAPKHPHGDSWITFAYVDRMTIS 120

Query: 121 GGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNILGCN 180
           G GVFDGQGKA WEKNDCH  I+CV+LPISLRFDFITNSIV+ ITSL+SKNFHVNILGCN
Sbjct: 121 GKGVFDGQGKAGWEKNDCHKRIDCVNLPISLRFDFITNSIVRGITSLDSKNFHVNILGCN 180

Query: 181 NLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNVTCG 240
           NLTF GVNIIAPE+SPNTDGIHIGRSIGISI KSRIATGDDCISLGDGSK+VKV NVTCG
Sbjct: 181 NLTFQGVNIIAPEDSPNTDGIHIGRSIGISILKSRIATGDDCISLGDGSKQVKVANVTCG 240

Query: 241 PGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           PGHGISIGSLGKYTNEEPV+ V VKNCT
Sbjct: 241 PGHGISIGSLGKYTNEEPVDDVEVKNCT 260

BLAST of Cla015663 vs. NCBI nr
Match: gi|703161510|ref|XP_010112809.1| (hypothetical protein L484_020041 [Morus notabilis])

HSP 1 Score: 317.0 bits (811), Expect = 3.2e-83
Identity = 155/271 (57.20%), Postives = 198/271 (73.06%), Query Frame = 1

Query: 1   MGVMKSSFLVTAFLMSLLSFIVESRSHVDVIFPLRKYGDIVPDADVTEALKDAWNDACAS 60
           MG+  S+F++ A L+ + S I  ++    V+F + + G I+P +D+T+AL  AWN+ACAS
Sbjct: 1   MGLKLSNFVIAALLLLVSSNIANAQI---VLFDVTENGTILPQSDITDALLKAWNNACAS 60

Query: 61  VRPSAVIIPKGIFKVSEVKFKGPCKSPIEIRLLGTLQAPKHPH----GDRWITFAYVDRL 120
             PS V++PKGIFK+S    +GPC +PIE  L G LQAP        GD W+ F YVD L
Sbjct: 61  TIPSKVVVPKGIFKLSGATLEGPCNAPIEFDLQGILQAPPEDGNFTGGDTWVAFEYVDLL 120

Query: 121 TLSGGGVFDGQGKAVWEKNDCHININCVDLPISLRFDFITNSIVKRITSLNSKNFHVNIL 180
           T+SGGG+FDGQGK  W K DC  +  C DLPI++RF+++TNSI++ ITSL+SK FH+N+L
Sbjct: 121 TISGGGIFDGQGKPTWGK-DCANDEYCGDLPINIRFNYVTNSIIQEITSLDSKQFHINVL 180

Query: 181 GCNNLTFHGVNIIAPENSPNTDGIHIGRSIGISITKSRIATGDDCISLGDGSKRVKVTNV 240
           GC N+TF  V IIA ENSP+TDGIHIGRS  I +  S I TGDDCISLGDGSK++ +  V
Sbjct: 181 GCQNVTFQHVKIIAAENSPHTDGIHIGRSTDIKVLDSNIQTGDDCISLGDGSKQITIAKV 240

Query: 241 TCGPGHGISIGSLGKYTNEEPVEGVIVKNCT 268
           TCGPGHGISIGSLGKY NEE V+GV VK+CT
Sbjct: 241 TCGPGHGISIGSLGKYLNEEAVKGVDVKSCT 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR_OENOR2.5e-7257.71Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1[more]
PGLR_GOSBA9.5e-7248.67Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1[more]
PGLR_GOSHI1.2e-7148.67Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1[more]
PGLR_MEDSA1.9e-6747.74Polygalacturonase OS=Medicago sativa PE=2 SV=1[more]
PGLR_TOBAC3.2e-6749.03Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K3B7_CUCSA6.1e-12684.70Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1[more]
W9SC16_9ROSA2.2e-8357.20Uncharacterized protein OS=Morus notabilis GN=L484_020041 PE=3 SV=1[more]
W9SBW1_9ROSA3.9e-8057.03Uncharacterized protein OS=Morus notabilis GN=L484_020040 PE=3 SV=1[more]
A0A147BN67_IXORI1.1e-7958.92Putative polygalacturonase/glycoside hydrolase family protein (Fragment) OS=Ixod... [more]
Q9MBB9_SALGI2.8e-7853.96Polygalacturonase OS=Salix gilgiana GN=SgPG2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
gi|659111053|ref|XP_008455553.1|3.4e-13086.19PREDICTED: exopolygalacturonase-like [Cucumis melo][more]
gi|700188231|gb|KGN43464.1|8.8e-12684.70hypothetical protein Csa_7G037910 [Cucumis sativus][more]
gi|778730103|ref|XP_011659699.1|2.0e-12286.17PREDICTED: exopolygalacturonase-like [Cucumis sativus][more]
gi|778730075|ref|XP_011659692.1|1.3e-12182.46PREDICTED: polygalacturonase-like [Cucumis sativus][more]
gi|703161510|ref|XP_010112809.1|3.2e-8357.20hypothetical protein L484_020041 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla015663Cla015663.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 58..267
score: 2.0
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 235..248
scor
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 44..267
score: 8.32
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 43..267
score: 2.6
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 4..267
score: 1.7E
NoneNo IPR availablePANTHERPTHR31375:SF37PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 4..267
score: 1.7E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cla015663Bhi05G001267Wax gourdwgowmB272
The following gene(s) are paralogous to this gene:

None