Cla015296 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla015296
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	4-coumarate CoA ligase (AHRD V1 ***- D1LZD3_EUCCA); contains Interpro domain(s) IPR000873 AMP-dependent synthetase and ligase
Location	Chr9 : 2697492 .. 2698038 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGGTATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGTGACATCGGCTTCGTGGACGACGACGATGAGATCTTCATTGTAGACCGGCTTAAGGAACTTATTAAATTTAAGGCCTTTCAAGTGGCGCCCGCCGAGCTGGAGGCCCTTCTTATCACCCACCCAAAACTATCTGACGCTGCTGTTATTGGGTGAGTTCAAATGAGTTTTGTTGTATATTGTAAGTAAAATAAATAATCATAGAGAGAAATTTGGTTTCATAACAGATATTTGGTTTTGGTTTTGTTTTTGTAGTATGCCGGATGTGGAGGCAGGAGAAGTGCCAGTGGCATTTGTGGTGAAGGCGAACGGTGGCGCTATAACAGAGGAAGAGGTAAAGCAATTCATAACAAAACAAGTGGTGTTCTACAAGAGGCTAAAACGTGTGTTTTTTGTCAACGCTATTCCAAAGGCTCCGTCTGGCAAAATCCTCAGAAAAGAACTCAGAGCAAAATTGGCTTCTGCTGCTTACAATTAA

mRNA sequence

ATGAAGGGGTATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGTGACATCGGCTTCGTGGACGACGACGATGAGATCTTCATTGTAGACCGGCTTAAGGAACTTATTAAATTTAAGGCCTTTCAAGTGGCGCCCGCCGAGCTGGAGGCCCTTCTTATCACCCACCCAAAACTATCTGACGCTGCTGTTATTGGTATGCCGGATGTGGAGGCAGGAGAAGTGCCAGTGGCATTTGTGGTGAAGGCGAACGGTGGCGCTATAACAGAGGAAGAGGTAAAGCAATTCATAACAAAACAAGTGGTGTTCTACAAGAGGCTAAAACGTGTGTTTTTTGTCAACGCTATTCCAAAGGCTCCGTCTGGCAAAATCCTCAGAAAAGAACTCAGAGCAAAATTGGCTTCTGCTGCTTACAATTAA

Coding sequence (CDS)

ATGAAGGGGTATTTGAATGATTTGGAGTCAACAAAGAGGACTATTGATAAAGAAGGATGGCTTCACACCGGTGACATCGGCTTCGTGGACGACGACGATGAGATCTTCATTGTAGACCGGCTTAAGGAACTTATTAAATTTAAGGCCTTTCAAGTGGCGCCCGCCGAGCTGGAGGCCCTTCTTATCACCCACCCAAAACTATCTGACGCTGCTGTTATTGGTATGCCGGATGTGGAGGCAGGAGAAGTGCCAGTGGCATTTGTGGTGAAGGCGAACGGTGGCGCTATAACAGAGGAAGAGGTAAAGCAATTCATAACAAAACAAGTGGTGTTCTACAAGAGGCTAAAACGTGTGTTTTTTGTCAACGCTATTCCAAAGGCTCCGTCTGGCAAAATCCTCAGAAAAGAACTCAGAGCAAAATTGGCTTCTGCTGCTTACAATTAA

Protein sequence

MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASAAYN

Homology

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BLAST of Cla015296 vs. Swiss-Prot
Match: 4CL_VANPL (4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 3.7e-62
Identity = 116/145 (80.00%), Postives = 131/145 (90.34%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T RTIDKEGWLHTGDIG++DDDDE+FIVDRLKELIK+K FQVAPAELEAL
Sbjct: 405 MKGYLNDPEATARTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 464

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           L+THP +SDAAV+ M D  AGEVPVAFVVK+NG  ITE+E+KQFI+KQV+FYKR+ RVFF
Sbjct: 465 LLTHPCISDAAVVPMKDEAAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFF 524

Query: 121 VNAIPKAPSGKILRKELRAKLASAA 146
           V AIPKAPSGKILRK+LRA+LA+AA
Sbjct: 525 VEAIPKAPSGKILRKDLRARLAAAA 549

[Close]

BLAST of Cla015296 vs. Swiss-Prot
Match: 4CL2_TOBAC (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 3.2e-61
Identity = 113/147 (76.87%), Postives = 131/147 (89.12%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T RTIDKEGWL+TGDIG++DDDDE+FIVDRLKELIK+K FQVAPAELEAL
Sbjct: 396 MKGYLNDPEATARTIDKEGWLYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           L+ HP +SDAAV+ M D +AGEVPVAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFF
Sbjct: 456 LLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           V+AIPK+PSGKILRK+LRAKLA+   N
Sbjct: 516 VDAIPKSPSGKILRKDLRAKLAAGLPN 542

[Close]

BLAST of Cla015296 vs. Swiss-Prot
Match: 4CL1_TOBAC (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 5.4e-61
Identity = 111/147 (75.51%), Postives = 130/147 (88.44%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T RTIDKEGWLHTGDIGF+D+DDE+FIVDRLKELIK+K FQVAPAE+EAL
Sbjct: 401 MKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEAL 460

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           L+ HP +SDAAV+ M D +AGEVPVAFVV++NG AITE+EVK FI+KQV+FYKR+KRVFF
Sbjct: 461 LLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFF 520

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           V  +PK+PSGKILRK+LRA+LA+   N
Sbjct: 521 VETVPKSPSGKILRKDLRARLAAGVPN 547

[Close]

BLAST of Cla015296 vs. Swiss-Prot
Match: 4CL1_SOLTU (4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 9.2e-61
Identity = 112/147 (76.19%), Postives = 129/147 (87.76%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T RTI+KEGWLHTGDIGF+DDDDE+FIVDRLKELIK+K FQVAPAELEAL
Sbjct: 399 MKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 458

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LI HP +SDAAV+ M D +AGEVPVAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFF
Sbjct: 459 LINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFF 518

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           V  +PK+PSGKILRK+LRA+LA+   N
Sbjct: 519 VETVPKSPSGKILRKDLRARLAAGISN 545

[Close]

BLAST of Cla015296 vs. Swiss-Prot
Match: 4CL2_SOLTU (4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 9.2e-61
Identity = 112/147 (76.19%), Postives = 129/147 (87.76%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T RTI+KEGWLHTGDIGF+DDDDE+FIVDRLKELIK+K FQVAPAELEAL
Sbjct: 399 MKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEAL 458

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LI HP +SDAAV+ M D +AGEVPVAFVV++NG  ITE+EVK FI+KQV+FYKR+KRVFF
Sbjct: 459 LINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFF 518

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           V  +PK+PSGKILRK+LRA+LA+   N
Sbjct: 519 VETVPKSPSGKILRKDLRARLAAGISN 545

[Close]

BLAST of Cla015296 vs. TrEMBL
Match: A0A0A0K658_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 1.7e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRT+DKEGWLHTGDIGFVDDDDE+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

[Close]

BLAST of Cla015296 vs. TrEMBL
Match: A0A0A0K8H9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 1.2e-72
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDD+E+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFV+KANGGAI+EEEVKQFI KQVVFYKRLKRVFF
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

[Close]

BLAST of Cla015296 vs. TrEMBL
Match: A0A0A0K6Q5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.1e-65
Identity = 129/150 (86.00%), Postives = 138/150 (92.00%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRTIDKEGWLHTGDIGF+DD++E+FI+DRLKELIKFK FQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTIDKEGWLHTGDIGFIDDNNELFIIDRLKELIKFKTFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGA---ITEEEVKQFITKQVVFYKRLKR 120
           LITHPKL DAAVIGMPDVEAGEVPVAFVV+   GA    TEEEVK+FI KQV+FYKRLKR
Sbjct: 456 LITHPKLRDAAVIGMPDVEAGEVPVAFVVEEKSGASATTTEEEVKEFIAKQVIFYKRLKR 515

Query: 121 VFFVNAIPKAPSGKILRKELRAKLASAAYN 148
           VFFVNAIPKAPSGKILRKE+RA LAS AYN
Sbjct: 516 VFFVNAIPKAPSGKILRKEIRANLASGAYN 545

[Close]

BLAST of Cla015296 vs. TrEMBL
Match: D1LZD3_EUCCA (4-coumarate:CoA ligase OS=Eucalyptus camaldulensis PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 3.8e-61
Identity = 116/147 (78.91%), Postives = 132/147 (89.80%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T  TIDKEGWLHTGDIG++DDDDE+FIVDRLKELIK+K FQVAPAELEA+
Sbjct: 398 MKGYLNDAEATANTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAM 457

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LI HP +SDAAV+ M D  AGEVPVAFVVK+NG  ITE+E+KQ+I+KQVVFYKR+KRVFF
Sbjct: 458 LIAHPSISDAAVVPMKDEVAGEVPVAFVVKSNGSVITEDEIKQYISKQVVFYKRIKRVFF 517

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
            +AIPKAPSGKILRK+LRAKLAS  YN
Sbjct: 518 TDAIPKAPSGKILRKDLRAKLASGVYN 544

[Close]

BLAST of Cla015296 vs. TrEMBL
Match: A0A059CRW7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02284 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 3.8e-61
Identity = 116/147 (78.91%), Postives = 132/147 (89.80%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLND E+T  TIDKEGWLHTGDIG++DDDDE+FIVDRLKELIK+K FQVAPAELEA+
Sbjct: 398 MKGYLNDAEATANTIDKEGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAM 457

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LI HP +SDAAV+ M D  AGEVPVAFVVK+NG  ITE+E+KQ+I+KQVVFYKR+KRVFF
Sbjct: 458 LIAHPSISDAAVVPMKDEVAGEVPVAFVVKSNGSVITEDEIKQYISKQVVFYKRIKRVFF 517

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
            +AIPKAPSGKILRK+LRAKLAS  YN
Sbjct: 518 TDAIPKAPSGKILRKDLRAKLASGVYN 544

[Close]

BLAST of Cla015296 vs. NCBI nr
Match: gi|778728828|ref|XP_004135893.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 286.6 bits (732), Expect = 2.5e-74
Identity = 144/147 (97.96%), Postives = 146/147 (99.32%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRT+DKEGWLHTGDIGFVDDDDE+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

[Close]

BLAST of Cla015296 vs. NCBI nr
Match: gi|659122577|ref|XP_008461216.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo])

HSP 1 Score: 284.3 bits (726), Expect = 1.2e-73
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRT+DKEGWLHTGDIGFVDDDDE+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTVDKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLA  AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLALGAYN 542

[Close]

BLAST of Cla015296 vs. NCBI nr
Match: gi|778728825|ref|XP_004135894.2| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus])

HSP 1 Score: 280.4 bits (716), Expect = 1.8e-72
Identity = 141/147 (95.92%), Postives = 145/147 (98.64%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDD+E+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDNELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFV+KANGGAI+EEEVKQFI KQVVFYKRLKRVFF
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVMKANGGAISEEEVKQFIAKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

[Close]

BLAST of Cla015296 vs. NCBI nr
Match: gi|659122579|ref|XP_008461217.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 277.3 bits (708), Expect = 1.5e-71
Identity = 141/147 (95.92%), Postives = 144/147 (97.96%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRTIDKEGWLHTGDIGFVD+D+E+FIVDRLKELIKFKAFQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTIDKEGWLHTGDIGFVDNDNELFIVDRLKELIKFKAFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LITHPKLSDAAVIGMPDVEAGEVPVAFVVKA GGAITEEEVKQFITKQVVFYKRLKRV F
Sbjct: 456 LITHPKLSDAAVIGMPDVEAGEVPVAFVVKAIGGAITEEEVKQFITKQVVFYKRLKRVLF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

[Close]

BLAST of Cla015296 vs. NCBI nr
Match: gi|659122581|ref|XP_008461218.1| (PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo])

HSP 1 Score: 266.9 bits (681), Expect = 2.1e-68
Identity = 133/147 (90.48%), Postives = 139/147 (94.56%), Query Frame = 1


Query: 1   MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDDDEIFIVDRLKELIKFKAFQVAPAELEAL 60
           MKGYLNDLESTKRTIDKEGWLHTGDIGFVDD++E+FI+DRLKELIKFK FQVAPAELEAL
Sbjct: 396 MKGYLNDLESTKRTIDKEGWLHTGDIGFVDDNNELFIIDRLKELIKFKTFQVAPAELEAL 455

Query: 61  LITHPKLSDAAVIGMPDVEAGEVPVAFVVKANGGAITEEEVKQFITKQVVFYKRLKRVFF 120
           LI HPKL DAAVIGMPDVEAGEVPVAFVV+   GA TEEEVKQF+TKQVVFYKRLKRVFF
Sbjct: 456 LIAHPKLRDAAVIGMPDVEAGEVPVAFVVEEKSGATTEEEVKQFVTKQVVFYKRLKRVFF 515

Query: 121 VNAIPKAPSGKILRKELRAKLASAAYN 148
           VNAIPKAPSGKILRKELRAKLAS AYN
Sbjct: 516 VNAIPKAPSGKILRKELRAKLASGAYN 542

The following BLAST results are available for this feature:

BLAST of Cla015296 vs. Swiss-Prot


Match Name	E-value	Identity	Description
4CL_VANPL	3.7e-62	80.00	4-coumarate--CoA ligase OS=Vanilla planifolia GN=4CL PE=3 SV=1	[more]
4CL2_TOBAC	3.2e-61	76.87	4-coumarate--CoA ligase 2 OS=Nicotiana tabacum GN=4CL2 PE=1 SV=1	[more]
4CL1_TOBAC	5.4e-61	75.51	4-coumarate--CoA ligase 1 OS=Nicotiana tabacum GN=4CL1 PE=2 SV=1	[more]
4CL1_SOLTU	9.2e-61	76.19	4-coumarate--CoA ligase 1 OS=Solanum tuberosum GN=4CL1 PE=3 SV=1	[more]
4CL2_SOLTU	9.2e-61	76.19	4-coumarate--CoA ligase 2 OS=Solanum tuberosum GN=4CL2 PE=3 SV=1	[more]

BLAST of Cla015296 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K658_CUCSA	1.7e-74	97.96	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430250 PE=4 SV=1	[more]
A0A0A0K8H9_CUCSA	1.2e-72	95.92	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430240 PE=4 SV=1	[more]
A0A0A0K6Q5_CUCSA	1.1e-65	86.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G430230 PE=4 SV=1	[more]
D1LZD3_EUCCA	3.8e-61	78.91	4-coumarate:CoA ligase OS=Eucalyptus camaldulensis PE=4 SV=1	[more]
A0A059CRW7_EUCGR	3.8e-61	78.91	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_C02284 PE=4 SV=1	[more]

BLAST of Cla015296 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778728828\|ref\|XP_004135893.2\|	2.5e-74	97.96	PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus]	[more]
gi\|659122577\|ref\|XP_008461216.1\|	1.2e-73	97.28	PREDICTED: 4-coumarate--CoA ligase 1-like [Cucumis melo]	[more]
gi\|778728825\|ref\|XP_004135894.2\|	1.8e-72	95.92	PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis sativus]	[more]
gi\|659122579\|ref\|XP_008461217.1\|	1.5e-71	95.92	PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo]	[more]
gi\|659122581\|ref\|XP_008461218.1\|	2.1e-68	90.48	PREDICTED: 4-coumarate--CoA ligase 2-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000873	AMP-dep_Synth/Lig
IPR025110	AMP-bd_C

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

Vocabulary: **Biological Process**
Term	Definition
GO:0008152	metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009805	coumarin biosynthetic process
biological_process	GO:0009809	lignin biosynthetic process
biological_process	GO:0009811	stilbene biosynthetic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0016207	4-coumarate-CoA ligase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0016874	ligase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU53630	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla015296	Cla015296.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU53630	WMU53630	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 1..47 score: 5.4
IPR025110	AMP-binding enzyme C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 56..131 score: 1.1
None	No IPR available	GENE3D	G3DSA:2.30.38.10		coord: 1..40 score: 4.5
None	No IPR available	GENE3D	G3DSA:3.30.300.30		coord: 44..143 score: 2.6
None	No IPR available	PANTHER	PTHR24096	FAMILY NOT NAMED	coord: 1..140 score: 8.1
None	No IPR available	PANTHER	PTHR24096:SF149	4-COUMARATE--COA LIGASE 1-RELATED	coord: 1..140 score: 8.1
None	No IPR available	unknown	SSF56801	Acetyl-CoA synthetase-like	coord: 1..143 score: 6.93

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla015296	Bhi09G000460	Wax gourd	wgowmB583

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla015296	Melon (DHL92) v3.6.1	medwmB194
Cla015296	Silver-seed gourd	carwmB0345
Cla015296	Silver-seed gourd	carwmB0451
Cla015296	Cucumber (Chinese Long) v3	cucwmB591
Cla015296	Watermelon (97103) v2	wmwmbB015
Cla015296	Watermelon (97103) v2	wmwmbB041
Cla015296	Wax gourd	wgowmB006
Cla015296	Cucumber (Gy14) v1	cgywmB031
Cla015296	Cucurbita maxima (Rimu)	cmawmB226
Cla015296	Cucurbita maxima (Rimu)	cmawmB328
Cla015296	Cucurbita maxima (Rimu)	cmawmB408
Cla015296	Cucurbita maxima (Rimu)	cmawmB857
Cla015296	Cucurbita moschata (Rifu)	cmowmB212
Cla015296	Cucurbita moschata (Rifu)	cmowmB322
Cla015296	Cucurbita moschata (Rifu)	cmowmB844
Cla015296	Wild cucumber (PI 183967)	cpiwmB192
Cla015296	Wild cucumber (PI 183967)	cpiwmB585
Cla015296	Melon (DHL92) v3.5.1	mewmB198
Cla015296	Watermelon (Charleston Gray)	wcgwmB007
Cla015296	Watermelon (Charleston Gray)	wcgwmB050
Cla015296	Cucumber (Chinese Long) v2	cuwmB566
Cla015296	Cucurbita pepo (Zucchini)	cpewmB131
Cla015296	Cucurbita pepo (Zucchini)	cpewmB312
Cla015296	Cucurbita pepo (Zucchini)	cpewmB536
Cla015296	Bottle gourd (USVL1VR-Ls)	lsiwmB176
Cla015296	Cucumber (Gy14) v2	cgybwmB528