Cla014826 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla014826
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Gibberellin 20-oxidase (AHRD V1 ***- Q9ARL2_LOLPR); contains Interpro domain(s) IPR005123 Oxoglutarate and iron-dependent oxygenase
Location	Chr9 : 6449893 .. 6451941 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGTGAACCATTACCCACTATGCCCAAACCCTAGCTTGACTTTGGGATTGAATCAACATTGTGATAGAAGCCTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAAATCTTCAAGGATGGCCATTGGATTTCTTTTGATCCTGTTGATGATGCCTTTTTTATCAACTTTGGCTATCTTTTCGAGGTAAATTGTGAAAAATATATTTTTGTACTTAAATGTTAAGATATATGTTACACTTTTAGTTCTAAATTTTTATATTTGCTTTCAATTTAGTCTCTAGGTTTCAAAATGCTTCAATTTTATTTTGAGATATGATTTTTGTTTTAATTTGATCCCTAAATTTCAAGATTTTTACATTTTCAACCTCGATTTTTTTTTTTTTTTTACTAAATATTCACTTCTGTATTTGGTATTAATATTTATTCATTAATTTAATATAATTTCAAAGTGAAATTTTAAAATTAGTTTTAATAGTAATGAAAAAAATTTAATAGATTACTAATTATAAAAAGTTTAATTGTTTATACCAAATTATTTCAATTAATTAGATGTATTTAATAGATAAGTAATTTGGTAATGGTTTCAATATACACAATTTTTTTTTTTTTTTAACAATGTGTGGGGTGGGATCAAACCTCTAACCTCGAGGTTGATAGTATATATTTGATGTTGGATGAGCTAGGCTCATTTTTGGTCAATAAATACACAATTACATTAATTTAATATATATCTTTATTTTTATTTTTGTAAAGAAAAAAAAATCAACTTTATAAATTACAATAGTAATATACTTGATGCCAAATTTGTGGGTATGGATGTTCATTTTCCCCATAGGGATGGGCCTCATGGGTCAGGGTGAGCATTCCCCACGACGAGGAATGTGTGAGGGAGAGGGGAGGAAAAATTCGCTGTAAGATAAATGGAAATAGGGACGGGGACGAAGAATGCATTCCTCGCCCCGGCCCACCCATTAGCCTCTTTTATTTATTTATTATGTTGATTTAACGTTATGTTATTATTATTATATAAATATTATTATCTAAATTTCAAGTTTTATTGTTTAATGGTTAAGATATTGAATATATTTGAATATAACCTTTAAATTTAGACTGCATGCGCGCGCGCACACATATATATATATATGAAGAATTTGATTGATAATTATTTCTTTCTACTAAATTAAAATTTGAGTAATTTCTCTTTAAATACATATTGTCTATGTAGAACTAATCATATTTAAAAATCTAGTGAGAAATTTAACCATTCAATTAAAATTTGTTCACGGTAGTGATTTTAAAACAAAAAATCGAACGCGGAAAAATTTCCCGCAATGAATCTCTTCCCTCCCCTGATCCGTGTGGAAAATTTTGCTGGAGAGAGAGAGAGAGAGAGCAAGTTCCTACCCATAACATATTTCATTTTTTGCACAAATTTTGGATCATTCCTACAAATCTCCCATACATAATATGGTTGATTATAAAATTAATTTAAATAACATAATTAATTTTGATATAATAAAAAAATGTATTTGAAAGAAAGATGGTTAAAAGAAATTAAAGACCAAATTAAAAGCAGAGATTTCAACCTCCAATTTTAAGAGGGTACATACCTCAATTTATATAGTTGCGTCATATATTGACTTTAGCAAAGTATTTAGATTATAAGTAGTCAATTAACAAAATGTTTAATAGTACAATACTATTGTTCGTTTATAATTAATATTCCGAAATGTGTTTTTGGTGTTATTAGAAGGTTCATTGTTATCCATACAAATAGAGTTTGTGTGGTCTTATATAGGTGATTAGCAATGGAAAGCTGAAGGCAACCGAACATCGAGTCGTAACAAATGCAAAGACATCTCGACAAAGCTTAGCATATTTTGTTTTTCCTGAGGATGATATGATAATAGAACCAGCAAAATGCTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTCAAGTTGGAAGACTTCAATACAAAATTTTATCCTGCATTAGCTAATAAAGAGGAAATTATGAAGTATATATTAGTTTAA

mRNA sequence

ATGTCAGTGAACCATTACCCACTATGCCCAAACCCTAGCTTGACTTTGGGATTGAATCAACATTGTGATAGAAGCCTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAAATCTTCAAGGATGGCCATTGGATTTCTTTTGATCCTGTTGATGATGCCTTTTTTATCAACTTTGGCTATCTTTTCGAGGTGATTAGCAATGGAAAGCTGAAGGCAACCGAACATCGAGTCGTAACAAATGCAAAGACATCTCGACAAAGCTTAGCATATTTTGTTTTTCCTGAGGATGATATGATAATAGAACCAGCAAAATGCTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTCAAGTTGGAAGACTTCAATACAAAATTTTATCCTGCATTAGCTAATAAAGAGGAAATTATGAAGTATATATTAGTTTAA

Coding sequence (CDS)

ATGTCAGTGAACCATTACCCACTATGCCCAAACCCTAGCTTGACTTTGGGATTGAATCAACATTGTGATAGAAGCCTCATCACCATCTTGTTTCAAGATGTCAATGGCCTTCAAATCTTCAAGGATGGCCATTGGATTTCTTTTGATCCTGTTGATGATGCCTTTTTTATCAACTTTGGCTATCTTTTCGAGGTGATTAGCAATGGAAAGCTGAAGGCAACCGAACATCGAGTCGTAACAAATGCAAAGACATCTCGACAAAGCTTAGCATATTTTGTTTTTCCTGAGGATGATATGATAATAGAACCAGCAAAATGCTTGATAAATGAAGCCAATCCTCCACATTATCGTTCCTTCAAGTTGGAAGACTTCAATACAAAATTTTATCCTGCATTAGCTAATAAAGAGGAAATTATGAAGTATATATTAGTTTAA

Protein sequence

MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLEDFNTKFYPALANKEEIMKYILV

Homology

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BLAST of Cla014826 vs. Swiss-Prot
Match: IDS3_HORVU (2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3)

HSP 1 Score: 129.4 bits (324), Expect = 3.1e-29
Identity = 59/125 (47.20%), Postives = 80/125 (64.00%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINF 60
           +++NHYP CPNP   LG   HCDR+LIT+L    VNGL++   G WI  DP  +AF +NF
Sbjct: 189 LNINHYPSCPNPDKALGQPPHCDRNLITLLLPGAVNGLEVSYKGDWIKVDPAPNAFVVNF 248

Query: 61  GYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSF 120
           G   EV++NG LK+ EHR +TN+  +R S+A F+ P  + +I PAK  +++ NPP YR+ 
Sbjct: 249 GQQLEVVTNGLLKSIEHRAMTNSALARTSVATFIMPTQECLIGPAKEFLSKENPPCYRTT 308

Query: 121 KLEDF 125
              DF
Sbjct: 309 MFRDF 313

[Close]

BLAST of Cla014826 vs. Swiss-Prot
Match: IDS2_HORVU (Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 1.7e-27
Identity = 60/128 (46.88%), Postives = 78/128 (60.94%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINF 60
           + +N+YP  PNPS TLGL  HCDR L+T+L    V GL+I   G WI   PV ++  INF
Sbjct: 188 VDINYYPPSPNPSRTLGLPPHCDRDLMTVLLPGAVPGLEIAYKGGWIKVQPVPNSLVINF 247

Query: 61  GYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSF 120
           G   EV++NG LKA EHR  TN    R S+A F+ P DD ++ PA+  ++E NPP YR+ 
Sbjct: 248 GLQLEVVTNGYLKAVEHRAATNFAEPRLSVASFIVPADDCVVGPAEEFVSEDNPPRYRTL 307

Query: 121 KLEDFNTK 128
            + +F  K
Sbjct: 308 TVGEFKRK 315

[Close]

BLAST of Cla014826 vs. Swiss-Prot
Match: DLO2_ARATH (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-27
Identity = 63/139 (45.32%), Postives = 85/139 (61.15%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINF 60
           M++N+YP CP P LT GL  H D +LIT+L QD V+GLQ+FKDG WI+ +PV + F +N 
Sbjct: 199 MAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNL 258

Query: 61  GYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEA--NPPHYR 120
           G   +VISN K K+  HR V N+   R S+  F  P +D +I PA+ LINE   +P  YR
Sbjct: 259 GDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYR 318

Query: 121 SFKLEDFNTKFYPALANKE 137
           +F   ++  KF+    + E
Sbjct: 319 NFTYAEYFEKFWDTAFDTE 337

[Close]

BLAST of Cla014826 vs. Swiss-Prot
Match: GAO1B_WHEAT (Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 2.7e-25
Identity = 61/135 (45.19%), Postives = 77/135 (57.04%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINF 60
           M +N+YP C  P  TLG   HCD + +TIL QD V GLQ+  +G W S  P  DAF +N 
Sbjct: 204 MRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNI 263

Query: 61  GYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSF 120
           G  F  +SNG+ K+  HR V N+K  R+SLA+F+ PE D ++ P   L++ ANP  Y  F
Sbjct: 264 GDTFMALSNGRYKSCLHRAVVNSKVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDF 323

Query: 121 ---KLEDFNTKFYPA 132
               L DF  K Y A
Sbjct: 324 TWRSLLDFTQKHYRA 338

[Close]

BLAST of Cla014826 vs. Swiss-Prot
Match: GAO1D_WHEAT (Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 8.0e-25
Identity = 60/135 (44.44%), Postives = 77/135 (57.04%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINF 60
           M +N+YP C  P  TLG   HCD + +TIL QD V GLQ+  +G W S  P  DAF +N 
Sbjct: 204 MRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHTEGRWRSIRPRADAFVVNI 263

Query: 61  GYLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSF 120
           G  F  +SNG+ K+  HR V N++  R+SLA+F+ PE D ++ P   L++ ANP  Y  F
Sbjct: 264 GDTFMALSNGRYKSCLHRAVVNSRVPRKSLAFFLCPEMDKVVAPPGTLVDAANPRAYPDF 323

Query: 121 ---KLEDFNTKFYPA 132
               L DF  K Y A
Sbjct: 324 TWRSLLDFTQKHYRA 338

[Close]

BLAST of Cla014826 vs. TrEMBL
Match: A0A0A0K4U9_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 1.3e-61
Identity = 109/143 (76.22%), Postives = 124/143 (86.71%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFG 60
           ++ NHYP+CPNPSLT+GLNQHCDR L+TILFQDV+GLQ+FKDGHWIS DP+DDAF +NFG
Sbjct: 190 LTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPIDDAFVVNFG 249

Query: 61  YLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFK 120
           YL EVISNGKLKA EHRVVTNA+ SRQS  Y +FPE +MIIEPAKCLINEANPPHYRSFK
Sbjct: 250 YLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFK 309

Query: 121 LEDFNTKFYPALANKEEIMKYIL 144
           +EDFN  F+    N EE MK+IL
Sbjct: 310 VEDFNRHFFAVSNNNEETMKFIL 332

[Close]

BLAST of Cla014826 vs. TrEMBL
Match: A0A0A0K5H3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 1.5e-49
Identity = 88/140 (62.86%), Postives = 112/140 (80.00%), Query Frame = 1


Query: 3   VNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYL 62
           VNHYP CPNPSLTLGL+QHCD SLITILFQDVNGLQ+ KDG WI   P+D+AF +N G++
Sbjct: 197 VNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFV 256

Query: 63  FEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLE 122
            ++I+NGKL+A EHR VTN+KTSRQSL Y V+P+D+ ++EPAKC+INEANPP YRS   +
Sbjct: 257 LQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRYRSLNFK 316

Query: 123 DFNTKFYPALANKEEIMKYI 143
           DF   + P   + + +M+YI
Sbjct: 317 DFQRNYLPRAVDTKAVMQYI 336

[Close]

BLAST of Cla014826 vs. TrEMBL
Match: A0A0A0K745_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 7.2e-49
Identity = 93/140 (66.43%), Postives = 111/140 (79.29%), Query Frame = 1


Query: 3   VNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYL 62
           V+HYP CPNPSLTLGLN+H D ++ITILFQDVNGLQ  KDG WI  DP+ DAF +N GY+
Sbjct: 193 VHHYPPCPNPSLTLGLNKHADPTVITILFQDVNGLQFLKDGEWIDVDPLPDAFLVNLGYV 252

Query: 63  FEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLE 122
           FEVISNGK+KA EHRVVTN  TSRQSLAY V+PE++M IEP+KC INEANPPHYR+ + +
Sbjct: 253 FEVISNGKMKAAEHRVVTNTTTSRQSLAYGVYPENNMTIEPSKCFINEANPPHYRTLEFK 312

Query: 123 DFNTKFYPALANKEEIMKYI 143
           DF  K      ++E  MK+I
Sbjct: 313 DFVNKLRSKHPDREMAMKFI 332

[Close]

BLAST of Cla014826 vs. TrEMBL
Match: A0A0A0K5H0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 6.1e-48
Identity = 90/139 (64.75%), Postives = 110/139 (79.14%), Query Frame = 1


Query: 4   NHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYLF 63
           +HYP CPNPSLT+GL+QH D +LITILFQDVNGLQ  KDG WI  DP+ DAF +N GY+F
Sbjct: 194 HHYPPCPNPSLTVGLSQHADPTLITILFQDVNGLQFLKDGQWIDVDPLPDAFLVNLGYVF 253

Query: 64  EVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLED 123
           EVISNGKLKA EHRVVTN+ TSRQ+L Y V+PE+D+ IEP+KC INEANPPHYRS + +D
Sbjct: 254 EVISNGKLKAAEHRVVTNSTTSRQTLVYVVYPENDVTIEPSKCFINEANPPHYRSLEFKD 313

Query: 124 FNTKFYPALANKEEIMKYI 143
           F  K      +++  M++I
Sbjct: 314 FYNKLMSKPPDRKTAMEFI 332

[Close]

BLAST of Cla014826 vs. TrEMBL
Match: A0A061DWT9_THECC (2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein, putative OS=Theobroma cacao GN=TCM_004072 PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 7.5e-38
Identity = 74/142 (52.11%), Postives = 103/142 (72.54%), Query Frame = 1


Query: 3   VNHYPLCPNPSLTLGLNQHCDRSLITILFQD-VNGLQIFKDGHWISFDPVDDAFFINFGY 62
           +NHYP CP PSLTLGL++H D S+ITIL Q  ++GLQ+FKDGHWI  +P+  AF +N G+
Sbjct: 192 INHYPPCPEPSLTLGLSKHRDPSIITILLQGHIHGLQVFKDGHWIGVEPLPHAFVVNIGF 251

Query: 63  LFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKL 122
           L ++ISNGKLK  EHRVVTN++ +R +++YFV+P D  ++EPAK L+N  NP  Y++FK 
Sbjct: 252 LLQIISNGKLKGAEHRVVTNSRDARTTISYFVYPSDQTLVEPAKALVNACNPTLYKAFKF 311

Query: 123 EDFNTKFYPALANKEEIMKYIL 144
            DF + F     N  E +K ++
Sbjct: 312 FDFVSAFLLKPGNTGEAVKELI 333

[Close]

BLAST of Cla014826 vs. NCBI nr
Match: gi|700189512|gb|KGN44745.1| (hypothetical protein Csa_7G376360 [Cucumis sativus])

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-61
Identity = 109/143 (76.22%), Postives = 124/143 (86.71%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFG 60
           ++ NHYP+CPNPSLT+GLNQHCDR L+TILFQDV+GLQ+FKDGHWIS DP+DDAF +NFG
Sbjct: 190 LTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPIDDAFVVNFG 249

Query: 61  YLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFK 120
           YL EVISNGKLKA EHRVVTNA+ SRQS  Y +FPE +MIIEPAKCLINEANPPHYRSFK
Sbjct: 250 YLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFK 309

Query: 121 LEDFNTKFYPALANKEEIMKYIL 144
           +EDFN  F+    N EE MK+IL
Sbjct: 310 VEDFNRHFFAVSNNNEETMKFIL 332

[Close]

BLAST of Cla014826 vs. NCBI nr
Match: gi|778727440|ref|XP_011659260.1| (PREDICTED: 2'-deoxymugineic-acid 2'-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 243.8 bits (621), Expect = 1.8e-61
Identity = 109/143 (76.22%), Postives = 124/143 (86.71%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFG 60
           ++ NHYP+CPNPSLT+GLNQHCDR L+TILFQDV+GLQ+FKDGHWIS DP+DDAF +NFG
Sbjct: 33  LTANHYPICPNPSLTMGLNQHCDRDLMTILFQDVSGLQVFKDGHWISIDPIDDAFVVNFG 92

Query: 61  YLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFK 120
           YL EVISNGKLKA EHRVVTNA+ SRQS  Y +FPE +MIIEPAKCLINEANPPHYRSFK
Sbjct: 93  YLLEVISNGKLKAVEHRVVTNAEASRQSFGYVIFPEKEMIIEPAKCLINEANPPHYRSFK 152

Query: 121 LEDFNTKFYPALANKEEIMKYIL 144
           +EDFN  F+    N EE MK+IL
Sbjct: 153 VEDFNRHFFAVSNNNEETMKFIL 175

[Close]

BLAST of Cla014826 vs. NCBI nr
Match: gi|659101502|ref|XP_008451637.1| (PREDICTED: 2'-deoxymugineic-acid 2'-dioxygenase-like [Cucumis melo])

HSP 1 Score: 237.7 bits (605), Expect = 1.3e-59
Identity = 106/143 (74.13%), Postives = 124/143 (86.71%), Query Frame = 1


Query: 1   MSVNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFG 60
           ++ NHYP+CPNPSLT+GLNQHCDR LITILFQDV+GLQ+FKDGHWIS DP++ AF +NFG
Sbjct: 33  ITTNHYPICPNPSLTMGLNQHCDRDLITILFQDVDGLQVFKDGHWISIDPIEGAFVVNFG 92

Query: 61  YLFEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFK 120
           YL EVISNGKLKA EHRVVTNAK SRQSL Y +FPE +MII+PA CLINEANPPHYRSFK
Sbjct: 93  YLLEVISNGKLKAVEHRVVTNAKASRQSLVYTIFPEKEMIIKPATCLINEANPPHYRSFK 152

Query: 121 LEDFNTKFYPALANKEEIMKYIL 144
           +EDFN  F+    N +E+MK++L
Sbjct: 153 VEDFNRHFFAVSNNNDEMMKFML 175

[Close]

BLAST of Cla014826 vs. NCBI nr
Match: gi|449458500|ref|XP_004146985.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus])

HSP 1 Score: 203.8 bits (517), Expect = 2.1e-49
Identity = 88/140 (62.86%), Postives = 112/140 (80.00%), Query Frame = 1


Query: 3   VNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYL 62
           VNHYP CPNPSLTLGL+QHCD SLITILFQDVNGLQ+ KDG WI   P+D+AF +N G++
Sbjct: 197 VNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDNAFVVNIGFV 256

Query: 63  FEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLE 122
            ++I+NGKL+A EHR VTN+KTSRQSL Y V+P+D+ ++EPAKC+INEANPP YRS   +
Sbjct: 257 LQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEAMMEPAKCMINEANPPRYRSLNFK 316

Query: 123 DFNTKFYPALANKEEIMKYI 143
           DF   + P   + + +M+YI
Sbjct: 317 DFQRNYLPRAVDTKAVMQYI 336

[Close]

BLAST of Cla014826 vs. NCBI nr
Match: gi|659100775|ref|XP_008451261.1| (PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo])

HSP 1 Score: 202.2 bits (513), Expect = 6.1e-49
Identity = 88/140 (62.86%), Postives = 110/140 (78.57%), Query Frame = 1


Query: 3   VNHYPLCPNPSLTLGLNQHCDRSLITILFQDVNGLQIFKDGHWISFDPVDDAFFINFGYL 62
           VNHYP CPNPSLTLGL+QHCD SLITILFQDVNGLQ+ KDG WI   P+D AF +N G++
Sbjct: 197 VNHYPPCPNPSLTLGLSQHCDPSLITILFQDVNGLQVLKDGQWIGVQPIDKAFVVNIGFV 256

Query: 63  FEVISNGKLKATEHRVVTNAKTSRQSLAYFVFPEDDMIIEPAKCLINEANPPHYRSFKLE 122
            ++I+NGKL+A EHR VTN+KTSRQSL Y V+P+D+  +EPAKC+INEANPP YRS   +
Sbjct: 257 LQIITNGKLQAAEHRAVTNSKTSRQSLTYLVYPKDEATMEPAKCMINEANPPRYRSLNFK 316

Query: 123 DFNTKFYPALANKEEIMKYI 143
           DF   + P   + + +M+YI
Sbjct: 317 DFQRNYLPRAVDTKAVMQYI 336

The following BLAST results are available for this feature:

BLAST of Cla014826 vs. Swiss-Prot


Match Name	E-value	Identity	Description
IDS3_HORVU	3.1e-29	47.20	2'-deoxymugineic-acid 2'-dioxygenase OS=Hordeum vulgare GN=IDS3 PE=1 SV=3	[more]
IDS2_HORVU	1.7e-27	46.88	Mugineic-acid 3-dioxygenase OS=Hordeum vulgare GN=IDS2 PE=2 SV=1	[more]
DLO2_ARATH	2.2e-27	45.32	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana GN=DLO2 PE=2 SV=1	[more]
GAO1B_WHEAT	2.7e-25	45.19	Gibberellin 20 oxidase 1-B OS=Triticum aestivum GN=GA20ox1B PE=2 SV=1	[more]
GAO1D_WHEAT	8.0e-25	44.44	Gibberellin 20 oxidase 1-D OS=Triticum aestivum GN=GA20ox1D PE=1 SV=1	[more]

BLAST of Cla014826 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K4U9_CUCSA	1.3e-61	76.22	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G376360 PE=3 SV=1	[more]
A0A0A0K5H3_CUCSA	1.5e-49	62.86	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G378370 PE=3 SV=1	[more]
A0A0A0K745_CUCSA	7.2e-49	66.43	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375860 PE=3 SV=1	[more]
A0A0A0K5H0_CUCSA	6.1e-48	64.75	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G375850 PE=3 SV=1	[more]
A0A061DWT9_THECC	7.5e-38	52.11	2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein, putative OS=T...	[more]

BLAST of Cla014826 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700189512\|gb\|KGN44745.1\|	1.8e-61	76.22	hypothetical protein Csa_7G376360 [Cucumis sativus]	[more]
gi\|778727440\|ref\|XP_011659260.1\|	1.8e-61	76.22	PREDICTED: 2'-deoxymugineic-acid 2'-dioxygenase-like [Cucumis sativus]	[more]
gi\|659101502\|ref\|XP_008451637.1\|	1.3e-59	74.13	PREDICTED: 2'-deoxymugineic-acid 2'-dioxygenase-like [Cucumis melo]	[more]
gi\|449458500\|ref\|XP_004146985.1\|	2.1e-49	62.86	PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis sativus]	[more]
gi\|659100775\|ref\|XP_008451261.1\|	6.1e-49	62.86	PREDICTED: hyoscyamine 6-dioxygenase-like [Cucumis melo]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005123	Oxoglu/Fe-dep_dioxygenase
IPR027443	IPNS-like

Vocabulary: **Molecular Function**
Term	Definition
GO:0016491	oxidoreductase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0016491	oxidoreductase activity

Unigene

This gene is associated with the following unigenes:


Unigene Name	Analysis Name	Sequence type in Unigene
WMU61851	watermelon EST collection version 2.0	transcribed_cluster
WMU64625	watermelon EST collection version 2.0	transcribed_cluster
WMU67119	watermelon EST collection version 2.0	transcribed_cluster
WMU67637	watermelon EST collection version 2.0	transcribed_cluster
WMU72180	watermelon EST collection version 2.0	transcribed_cluster

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla014826	Cla014826.1	mRNA

The following transcribed_cluster feature(s) are associated with this gene:


Feature Name	Unique Name	Type
WMU72180	WMU72180	transcribed_cluster
WMU67637	WMU67637	transcribed_cluster
WMU64625	WMU64625	transcribed_cluster
WMU67119	WMU67119	transcribed_cluster
WMU61851	WMU61851	transcribed_cluster

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PFAM	PF03171	2OG-FeII_Oxy	coord: 2..95 score: 2.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROFILE	PS51471	FE2OG_OXY	coord: 1..95 score: 13
IPR027443	Isopenicillin N synthase-like	GENE3D	G3DSA:2.60.120.330		coord: 1..133 score: 4.9
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..139 score: 8.5
None	No IPR available	PANTHER	PTHR10209:SF124	SUBFAMILY NOT NAMED	coord: 1..139 score: 8.5
None	No IPR available	unknown	SSF51197	Clavaminate synthase-like	coord: 1..130 score: 5.29

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla014826	ClCG09G007720	Watermelon (Charleston Gray)	wcgwmB007

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla014826	Wild cucumber (PI 183967)	cpiwmB586
Cla014826	Melon (DHL92) v3.5.1	mewmB205
Cla014826	Watermelon (Charleston Gray)	wcgwmB271
Cla014826	Cucumber (Chinese Long) v2	cuwmB567
Cla014826	Cucurbita pepo (Zucchini)	cpewmB311
Cla014826	Cucurbita pepo (Zucchini)	cpewmB314
Cla014826	Cucurbita pepo (Zucchini)	cpewmB823
Cla014826	Bottle gourd (USVL1VR-Ls)	lsiwmB175
Cla014826	Cucumber (Gy14) v2	cgybwmB529
Cla014826	Melon (DHL92) v3.6.1	medwmB201
Cla014826	Silver-seed gourd	carwmB0215
Cla014826	Silver-seed gourd	carwmB0959
Cla014826	Cucumber (Chinese Long) v3	cucwmB592
Cla014826	Watermelon (97103) v2	wmwmbB041
Cla014826	Wax gourd	wgowmB580
Cla014826	Wax gourd	wgowmB592
Cla014826	Cucurbita moschata (Rifu)	cmowmB843
Cla014826	Cucumber (Gy14) v1	cgywmB702
Cla014826	Cucurbita maxima (Rimu)	cmawmB333
Cla014826	Cucurbita maxima (Rimu)	cmawmB778
Cla014826	Cucurbita maxima (Rimu)	cmawmB856