Cla011547 (gene) Watermelon (97103) v1

BLAST of Cla011547 vs. Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.8e-94
Identity = 190/485 (39.18%), Postives = 283/485 (58.35%), Query Frame = 1

Query: 45  RRFRLPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVARE 104
           RR RLPPGP G P++G LP L    H    ++ KKYGPI+ L++G    ++ ++P+ A+ 
Sbjct: 32  RRRRLPPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPNAAKA 91

Query: 105 ILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYEL 164
            LK  D+ F+NR          Y   D+ + PYGP W++LRK+  + ML    L++   +
Sbjct: 92  FLKTLDINFSNRPPNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANV 151

Query: 165 RHREVKNTVAYLY--RRAGSAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQ 224
           R  E+ + +  ++     G  + V + +   + N++  ++    V  +K ++    EF+ 
Sbjct: 152 RANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRVFVEKGKE--VNEFKN 211

Query: 225 TISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEG 284
            + ++  + G  N+ DF P +A  D QGIEK M KL  K D +  KM +E    ++  +G
Sbjct: 212 MVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDLLTKMFEEHEATSNERKG 271

Query: 285 AKKNDFLQFLLKVKDEGDSKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKV 344
             K DFL F++  +D  + +  L++  +K LL+++   GTDTSS+ IE+A+ EMMKNP +
Sbjct: 272 --KPDFLDFIMANRDNSEGER-LSITNIKALLLNLFTAGTDTSSSVIEWALTEMMKNPTI 331

Query: 345 LEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKY 404
            +KA++E+  ++G+     ES I +LPYL+AI KE  R HP  PL +P   S    I  Y
Sbjct: 332 FKKAQQEMDQIIGKNRRFIESDIPNLPYLRAICKEAFRKHPSTPLNLPRVSSDACTIDGY 391

Query: 405 TIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFF---NDQFDFSGSDFRYLPFGSGRRNC 464
            IPK +R+  N+WAI RDP+ WENPLEF+PERF    N + +  G+DF  +PFG+GRR C
Sbjct: 392 YIPKNTRLSVNIWAIGRDPDVWENPLEFIPERFLSEKNAKIEHRGNDFELIPFGAGRRIC 451

Query: 465 AGIAMAERMVMYLLATLLHSFDWKL-NEVEKIEIEEKFGIVLKMKKPLVLIPKPRLSDPT 524
           AG  M   MV Y+L TL+HSFDWKL N+V  I +EE FG+ L+   PL  I  PRLS   
Sbjct: 452 AGTRMGIVMVEYILGTLIHSFDWKLPNDVVDINMEETFGLALQKAVPLEAIVTPRLSF-D 510

HSP 2 Score: 115.2 bits (287), Expect = 3.3e-24
Identity = 76/217 (35.02%), Postives = 114/217 (52.53%), Query Frame = 1

Query: 576 GVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFA 635
           G PV+G LP L    H    ++ +KYGPI+ L++G    ++ ++P  A+  LK  DI F+
Sbjct: 42  GWPVIGALPLLGGMPHVALAKMAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFS 101

Query: 636 NRDVPQAGRV-ATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV 695
           NR  P AG     Y   D+ + PYGP W++LRK+  + ML   +L++   +R     N +
Sbjct: 102 NRP-PNAGATHMAYNAQDMVFAPYGPRWKLLRKLSNLHMLGGKALENWANVR----ANEL 161

Query: 696 GHLYRRAGAAVNVGEQ----GFLT--VFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMT 755
           GH+ +    A +VGE+      LT  + N+I  ++    V   +K     EF+ +V ++ 
Sbjct: 162 GHMLKSMFDASHVGERIVVADMLTFAMANMIGQVMLSKRV-FVEKGKEVNEFKNMVVELM 221

Query: 756 ELAGKPNISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
            +AG  NI DF P  A  DLQGIEK + KL  K D +
Sbjct: 222 TVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHKKFDDL 252

BLAST of Cla011547 vs. Swiss-Prot
Match: C76B6_CATRO (Geraniol 8-hydroxylase OS=Catharanthus roseus GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.6e-93
Identity = 189/491 (38.49%), Postives = 286/491 (58.25%), Query Frame = 1

Query: 26  FLLTLFTAFLLLLYVKYWPRRFR-LPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIV 85
           F LTL+ AF       Y  RR + LPPGP  +P +G+L  L  + H    +L KK+GPI+
Sbjct: 12  FALTLYEAF------SYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIM 71

Query: 86  KLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRML 145
            L+LG    I+++S ++A+E+L+  D+ F++R VP A        F + W P    WR L
Sbjct: 72  SLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSL 131

Query: 146 RKICTIKMLSNAGLDSVYELRHREVKNTVAYLYR--RAGSAVNVGEQVFLTVFNVVTSML 205
           RK+    + S   LD+   LR R+V+  +AY  +  ++G AV+VG   F T  N++++++
Sbjct: 132 RKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLI 191

Query: 206 WGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKL 265
           +   +  D   D  A EF+  + +I    GKPN+ DFFP L + D QGI  +M     ++
Sbjct: 192 FSKDLT-DPYSDS-AKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEV 251

Query: 266 DTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEGDSKTPLTMVQLKGLLMDMVIGGT 325
             +F  +++ERL+        +KND L  LL    E   +   T ++   + +D+ + GT
Sbjct: 252 LKLFGGLVNERLEQRR--SKGEKNDVLDVLLTTSQESPEEIDRTHIER--MCLDLFVAGT 311

Query: 326 DTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLRLH 385
           DT+S+T+E+AM+EM+KNP  ++K ++ELA V+G    +EES I  LPYL+ +MKETLR+H
Sbjct: 312 DTTSSTLEWAMSEMLKNPDKMKKTQDELAQVIGRGKTIEESDINRLPYLRCVMKETLRIH 371

Query: 386 PILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFFNDQFDF 445
           P +P L+P    Q+  +  Y +PKGS+V  N WAI RD   W++ L F PERF   + D 
Sbjct: 372 PPVPFLIPRKVEQSVEVCGYNVPKGSQVLVNAWAIGRDETVWDDALAFKPERFMESELDI 431

Query: 446 SGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLN---EVEKIEIEEKFGIV 505
            G DF  +PFG+GRR C G+ +A R V  +L +LL+SF+WKL      + +++EEKFGI 
Sbjct: 432 RGRDFELIPFGAGRRICPGLPLALRTVPLMLGSLLNSFNWKLEGGMAPKDLDMEEKFGIT 490

Query: 506 LKMKKPLVLIP 511
           L+   PL  +P
Sbjct: 492 LQKAHPLRAVP 490

HSP 2 Score: 127.9 bits (320), Expect = 5.0e-28
Identity = 69/201 (34.33%), Postives = 112/201 (55.72%), Query Frame = 1

Query: 577 VPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFAN 636
           +P +G+L  L  + H    +L +K+GPI+ L+LG    I+++S T+A+EVL+  D+ F++
Sbjct: 37  LPFIGSLHLLGDQPHKSLAKLSKKHGPIMSLKLGQITTIVISSSTMAKEVLQKQDLAFSS 96

Query: 637 RDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGH 696
           R VP A        F + W P    WR LRKV    + S   LD+   LR R+V+  + +
Sbjct: 97  RSVPNALHAHNQFKFSVVWLPVASRWRSLRKVLNSNIFSGNRLDANQHLRTRKVQELIAY 156

Query: 697 LYR--RAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 756
             +  ++G AV+VG   F T  N+++++++   +     D+ A EF+ LV ++   AGKP
Sbjct: 157 CRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDS-AKEFKDLVWNIMVEAGKP 216

Query: 757 NISDFFPSFARFDLQGIEKQV 776
           N+ DFFP   + D QGI  ++
Sbjct: 217 NLVDFFPLLEKVDPQGIRHRM 236

BLAST of Cla011547 vs. Swiss-Prot
Match: C76BA_SWEMU (Geraniol 8-hydroxylase OS=Swertia mussotii GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 9.9e-93
Identity = 190/493 (38.54%), Postives = 286/493 (58.01%), Query Frame = 1

Query: 26  FLLTLFTAFLLLLYVKYWPRRFR-LPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIV 85
           F +TL+ A      + ++ R+ + LPPGP  +PL+GNL  L  + H    +L KK+GPI+
Sbjct: 14  FTITLYQA------LNFFSRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIM 73

Query: 86  KLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRML 145
            LQLG    I+V S  +A+E+L+  D+ F++R +P A        + + W P    WR L
Sbjct: 74  GLQLGQVTTIVVTSSGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGL 133

Query: 146 RKICTIKMLSNAGLDSVYELRHREVKNTVAYLYR--RAGSAVNVGEQVFLTVFNVVTSML 205
           RK     M S   LD+   LR R+V+  +AY  +  + G A++VG   F T  N++++ +
Sbjct: 134 RKALNSNMFSGNRLDANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTM 193

Query: 206 WGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKL 265
           +   +  D   D  A EF+  + ++    GKPN+ D+FP L + D QGI K+M     K+
Sbjct: 194 FSKDLT-DPYSDS-AKEFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKI 253

Query: 266 DTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEGDSKTPLTMVQLKGLLMDMVIGGT 325
             +F  +IDERL+          +D L  LL   +E   +   T +Q   + +D+ + GT
Sbjct: 254 LELFGGLIDERLQQKKAK--GVNDDVLDVLLTTSEESPEEIDRTHIQR--MCLDLFVAGT 313

Query: 326 DTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLRLH 385
           DT+S+T+E+AM+EM+KNP+ ++ A+ ELA V+G+   VEE+ +  LPYL+  +KETLR+H
Sbjct: 314 DTTSSTLEWAMSEMLKNPEKMKAAQAELAQVIGKGKAVEEADLARLPYLRCAIKETLRIH 373

Query: 386 PILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFFNDQFDF 445
           P +PLL+P    Q   +  YT+PK S+V  NVWAI RD   W++PL F PERF   + + 
Sbjct: 374 PPVPLLIPRRTEQEVEVCGYTVPKNSQVLVNVWAISRDDAIWKDPLSFKPERFLESELEM 433

Query: 446 SGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLN---EVEKIEIEEKFGIV 505
            G DF  +PFG+GRR C G+ +A RMV  +L +LL+SFDWKL      + +++EEKFGI 
Sbjct: 434 RGKDFELIPFGAGRRICPGLPLAVRMVPVMLGSLLNSFDWKLEGGIAPKDLDMEEKFGIT 493

Query: 506 LKMKKPLVLIPKP 513
           L+   PL  +  P
Sbjct: 494 LQKAHPLRAVATP 494

HSP 2 Score: 123.6 bits (309), Expect = 9.4e-27
Identity = 67/201 (33.33%), Postives = 109/201 (54.23%), Query Frame = 1

Query: 577 VPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFAN 636
           +P++GNL  L  + H    +L +K+GPI+ LQLG    I++ S  +A+EVL+  D+ F++
Sbjct: 39  LPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTSSGMAKEVLQKQDLAFSS 98

Query: 637 RDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGH 696
           R +P A        + + W P    WR LRK     M S   LD+   LR R+V+  + +
Sbjct: 99  RSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRLDANQHLRSRKVQELIAY 158

Query: 697 LYR--RAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 756
             +  + G A++VG   F T  N++++ ++   +     D+ A EF+ LV ++   AGKP
Sbjct: 159 CRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDS-AKEFKDLVWNVMVEAGKP 218

Query: 757 NISDFFPSFARFDLQGIEKQV 776
           N+ D+FP   + D QGI K++
Sbjct: 219 NLVDYFPLLDKVDPQGIRKRM 238

BLAST of Cla011547 vs. Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.9e-92
Identity = 197/509 (38.70%), Postives = 289/509 (56.78%), Query Frame = 1

Query: 28  LTLFTAFLLLLYVKYWPRRF------RLPPGPPGVPLLGNLPFLDPELHTYFTELGKKYG 87
           LT+  A +L L ++ +  R       RLPPGP   P++GNLP +  + H   + +   YG
Sbjct: 6   LTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVTTYG 65

Query: 88  PIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEW 147
           PI+ L+LG    ++  S SVA + LK HD  FA+R      +   Y   D+ + PYG  W
Sbjct: 66  PILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRW 125

Query: 148 RMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGSAVNVGEQVFLTVFNVVTSM 207
           R+LRKI ++ + S   L+    +R  EV      L R     VN+G+ V + V N +   
Sbjct: 126 RLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGRE 185

Query: 208 LWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLK 267
           + G  + G    D  A EFR  ++++  L G  N+ DF PSL   D QG+  +M +L  +
Sbjct: 186 MIGRRLFGADA-DHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKR 245

Query: 268 LDTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKD---EGDSKTPLTMVQLKGLLMDMV 327
            D     ++ E  +M   D+  K  D L  L+ +K    +GD  + LT  ++K LL++M 
Sbjct: 246 FDAFLSSILKEH-EMNGQDQ--KHTDMLSTLISLKGTDLDGDGGS-LTDTEIKALLLNMF 305

Query: 328 IGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKET 387
             GTDTS++T+++A+AE++++P ++ KA+EEL IVVG    V ES I  LPYL+A++KE 
Sbjct: 306 TAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKEN 365

Query: 388 LRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFF-- 447
            RLHP  PL +PH  S++  I+ Y IPKGS +  N+WAI RDP+ W +PL F PERF   
Sbjct: 366 FRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPG 425

Query: 448 --NDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLN---EVEKIE 507
                 D  GSDF  +PFG+GRR CAG+++  R + +L ATL+  FDW+L      EK+ 
Sbjct: 426 GEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLN 485

Query: 508 IEEKFGIVLKMKKPLVLIPKPRLSDPTLY 521
           +EE +G+ L+   PLV+ PKPRL+ P +Y
Sbjct: 486 MEESYGLTLQRAVPLVVHPKPRLA-PNVY 508

HSP 2 Score: 123.2 bits (308), Expect = 1.2e-26
Identity = 69/206 (33.50%), Postives = 106/206 (51.46%), Query Frame = 1

Query: 578 PVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFANR 637
           P++GNLP +  + H   + +   YGPI+ L+LG    ++  S +VA + LK HD  FA+R
Sbjct: 41  PIIGNLPHMGTKPHRTLSAMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASR 100

Query: 638 DVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGHL 697
                 +   Y   D+ + PYG  WR+LRK+ ++ + S  +L+    +R+ EV      L
Sbjct: 101 PPNSGAKHMAYNYQDLVFAPYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTREL 160

Query: 698 YRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKPNIS 757
            R     VN+G+   + V N +   + G  + G   D  A EFR +V++M  LAG  NI 
Sbjct: 161 VRVGTKPVNLGQLVNMCVVNALGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIG 220

Query: 758 DFFPSFARFDLQGIEKQVLKLVPKLD 784
           DF PS    DLQG+  ++ +L  + D
Sbjct: 221 DFVPSLDWLDLQGVAGKMKRLHKRFD 246

BLAST of Cla011547 vs. Swiss-Prot
Match: C71A1_PERAE (Cytochrome P450 71A1 OS=Persea americana GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 340.1 bits (871), Expect = 6.4e-92
Identity = 195/498 (39.16%), Postives = 289/498 (58.03%), Query Frame = 1

Query: 26  FLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIVK 85
           FL    T FLL L  K   ++  LPP PP +P++GNL  L    H     L  + GP++ 
Sbjct: 9   FLAIALTFFLLKLNEKR-EKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELGPLIL 68

Query: 86  LQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRMLR 145
           L LG    +IV++  +A EILK HD+IFA+R    A R   Y   D++++PYG  WR +R
Sbjct: 69  LHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVR 128

Query: 146 KICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRA--GSAVNVGEQVFLTVFNVVTSMLW 205
           KIC +++LS   ++S   +R  EV   +  + +    G AVN+ E + L     +T + +
Sbjct: 129 KICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRVAF 188

Query: 206 GGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFD-FQGIEKQMLKLVLKL 265
           G   +G++ R     +F    +++T L+G   V D+FPS A  D   G++ ++ +   +L
Sbjct: 189 GKKYEGEEERKN---KFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGEL 248

Query: 266 DTIFEKMIDERL--KMTDVDEGAKKNDFLQFLLKVKDEGDSKTPLTMVQLKGLLMDMVIG 325
           D   + +ID+ L  +  +  +G ++ D +  LL ++ +      L    LK +++DM  G
Sbjct: 249 DAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSG 308

Query: 326 GTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLR 385
           GTDT++ T+E+AMAE++K+P V+EKA++E+  VVG++  VEE  +  L YLK I+KETLR
Sbjct: 309 GTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLR 368

Query: 386 LHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFFNDQF 445
           LHP+ PLLVP   ++  VI  Y IP  +RVF N WAI RDP +WEN  EF+PERF N+  
Sbjct: 369 LHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNSV 428

Query: 446 DFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLN---EVEKIEIEEKFG 505
           DF G DF+ +PFG+GRR C GIA     V   LA LL+ F+W+L      E +++ E  G
Sbjct: 429 DFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVG 488

Query: 506 IVLKMKKPLVLIPKPRLS 516
           I + MK PL L+ K  LS
Sbjct: 489 ITVHMKFPLQLVAKRHLS 502

HSP 2 Score: 109.4 bits (272), Expect = 1.8e-22
Identity = 67/210 (31.90%), Postives = 111/210 (52.86%), Query Frame = 1

Query: 577 VPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFAN 636
           +P++GNL  L    H     L  + GP++ L LG    +I+++  +A E+LK HD+ FA+
Sbjct: 38  LPIIGNLHQLGNLPHRSLRSLANELGPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFAS 97

Query: 637 RDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGH 696
           R    A R   Y   D+ ++PYG  WR +RK+C +++LS   ++S   +R  EV   +  
Sbjct: 98  RPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGLMMER 157

Query: 697 LYR--RAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 756
           + +    G AVN+ E   L     IT + +G   EG+++     +F  L +++T L G  
Sbjct: 158 ISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEGEEER--KNKFADLATELTTLMGAF 217

Query: 757 NISDFFPSFARFD-LQGIEKQVLKLVPKLD 784
            + D+FPSFA  D L G++ ++ +   +LD
Sbjct: 218 FVGDYFPSFAWVDVLTGMDARLKRNHGELD 245

BLAST of Cla011547 vs. TrEMBL
Match: A0A0A0KKS3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 1.6e-251
Identity = 432/521 (82.92%), Postives = 467/521 (89.64%), Query Frame = 1

Query: 1   MEPTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLG 60
           M  TSNFLHSL    +F DPNN+T FLLTLF  FLLLLYVK+  RR  LPPGPPGVPLLG
Sbjct: 1   MALTSNFLHSL----DFFDPNNKTHFLLTLFAGFLLLLYVKFSRRRIHLPPGPPGVPLLG 60

Query: 61  NLPFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQ 120
           NLPFLDPELH+YFTELG+KYGPIV+LQLGGKIGIIVNS S+ARE+LKDHD+ FAN DV +
Sbjct: 61  NLPFLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHE 120

Query: 121 AGRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRA 180
           AGR ATYGG DISW PYGPEWRMLRK+CTIKMLSNA LDSVYELR REV+NTVA+LYRRA
Sbjct: 121 AGRAATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRA 180

Query: 181 GSAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFF 240
           GSAVNVGEQ FLT+FNVVTSMLWGGSV+ D+ RD +AAEFR+TIS+IT LLG+PNVSDFF
Sbjct: 181 GSAVNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFF 240

Query: 241 PSLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEGD 300
           PSLARFD QGIEKQM+K VLKLDTIFEKMIDER++M DV+E  KKNDFLQFLL VKDEGD
Sbjct: 241 PSLARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNESVKKNDFLQFLLTVKDEGD 300

Query: 301 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIV 360
           SKTPLTMVQLKGLLMDMV GGTDTSSNT+EFAM EMMKNPK+LEKAKEEL  VVGEQ +V
Sbjct: 301 SKTPLTMVQLKGLLMDMVTGGTDTSSNTVEFAMGEMMKNPKILEKAKEELCAVVGEQRMV 360

Query: 361 EESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRD 420
           EESHIQSL YLKA+MKETLRLHPILPLL+PH PS+TTV+S YTIPKGSRVF NVWAI RD
Sbjct: 361 EESHIQSLSYLKAVMKETLRLHPILPLLIPHCPSETTVVSNYTIPKGSRVFVNVWAIQRD 420

Query: 421 PNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSF 480
           PN WENPL F PERF N +FDFSGSDFRY PFGSGRRNCAGIAMAER VMYLLATLLHSF
Sbjct: 421 PNEWENPLVFDPERFLNGKFDFSGSDFRYFPFGSGRRNCAGIAMAERTVMYLLATLLHSF 480

Query: 481 DWKLNEVEKIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           DWKL E EKIE+EEKFGIVLKMKKPLVLIP+PRL DPTLY+
Sbjct: 481 DWKLEEGEKIEVEEKFGIVLKMKKPLVLIPRPRLLDPTLYE 517

BLAST of Cla011547 vs. TrEMBL
Match: A0A0A0KKS3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159620 PE=3 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.5e-95
Identity = 184/261 (70.50%), Postives = 210/261 (80.46%), Query Frame = 1

Query: 534 SPFHHGTHFQIPSNSYPLSSHLLRRVSTNFSLCQTLAAALAR--------GVPVLGNLPF 593
           S F H   F  P+N     +H L  +   F L   +  +  R        GVP+LGNLPF
Sbjct: 5   SNFLHSLDFFDPNNK----THFLLTLFAGFLLLLYVKFSRRRIHLPPGPPGVPLLGNLPF 64

Query: 594 LDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFANRDVPQAGRV 653
           LDPELH+YFTELGRKYGPIV+LQLGGKIGII+NS ++AREVLKDHDITFAN DV +AGR 
Sbjct: 65  LDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGRA 124

Query: 654 ATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGHLYRRAGAAV 713
           ATYGG DI+W PYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV HLYRRAG+AV
Sbjct: 125 ATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSAV 184

Query: 714 NVGEQGFLTVFNVITSMLWGGTVEGDQ-KDAFAAEFRGLVSDMTELAGKPNISDFFPSFA 773
           NVGEQGFLT+FNV+TSMLWGG+VE DQ +D  AAEFR  +S++T+L G+PN+SDFFPS A
Sbjct: 185 NVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSLA 244

Query: 774 RFDLQGIEKQVLKLVPKLDTI 786
           RFDLQGIEKQ++K V KLDTI
Sbjct: 245 RFDLQGIEKQMVKHVLKLDTI 261

HSP 2 Score: 808.1 bits (2086), Expect = 9.2e-231
Identity = 403/512 (78.71%), Postives = 444/512 (86.72%), Query Frame = 1

Query: 12  IHSLNFIDPNNQTRFLLTLFTAFLLL-LYVKYWPRRFRLPPGPPGVPLLGNLPFLDPELH 71
           + +LN  +PNNQT FLL LF  FLL+ LYVK    R +LPPGP GVPLLGNLPFLDP LH
Sbjct: 4   LQTLNSYNPNNQTGFLLALFAGFLLIFLYVKLSQPRLQLPPGPRGVPLLGNLPFLDPNLH 63

Query: 72  TYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGF 131
           TYF ELG+KYG IVKLQLGGK+GIIVNSPSVAREILKDHD+ FANRDVPQAGR+ATYGGF
Sbjct: 64  TYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGF 123

Query: 132 DISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGSAVNVGEQV 191
           DI+WTPYGPEWRMLRK+CTIK+L NA LD VYELR  EV+ TVA LY+RA S V +GEQV
Sbjct: 124 DITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQV 183

Query: 192 FLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQG 251
           F TVFNV+TSMLWGG+++G++ +  +A  FR+ +S +T L GKPN+SDFFPSLA  D QG
Sbjct: 184 FFTVFNVITSMLWGGTMEGEE-KAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQG 243

Query: 252 IEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGAK-KNDFLQFLLKVKDEGDSKTPLTMVQ 311
           IEK+MLKL+ KLDTIFEK+IDERL+MT+ +EG++ KNDFLQFLLKVKDE DS+TPLT+VQ
Sbjct: 244 IEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQ 303

Query: 312 LKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLP 371
           LK LLMDMV GGTDTSSNTIEFAMAEMMKNPKV EKAKEEL  VVGEQ+IVEESHIQSLP
Sbjct: 304 LKALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLP 363

Query: 372 YLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLE 431
           YLKAIMKETLRLHPILPLLVPH PS TTV+S YTIPKGSRVF NVWAI RDPN WENPLE
Sbjct: 364 YLKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLE 423

Query: 432 FVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLNEVEK 491
           F PERF N + DFSGSDFRY PFGSGRR C GIAM ERMVMYLLATLLHSFDWKL E E+
Sbjct: 424 FDPERFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEE 483

Query: 492 IEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           IEIEEKFGIVL MKKPLVLIP PRLSDPTLYQ
Sbjct: 484 IEIEEKFGIVLTMKKPLVLIPTPRLSDPTLYQ 514

BLAST of Cla011547 vs. TrEMBL
Match: A0A0A0KP59_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159610 PE=3 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.4e-93
Identity = 170/211 (80.57%), Postives = 188/211 (89.10%), Query Frame = 1

Query: 575 RGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITF 634
           RGVP+LGNLPFLDP LHTYF ELG+KYG IVKLQLGGK+GII+NSP+VARE+LKDHDITF
Sbjct: 47  RGVPLLGNLPFLDPNLHTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITF 106

Query: 635 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV 694
           ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIK+L NASLD VYELRR EVR TV
Sbjct: 107 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTV 166

Query: 695 GHLYRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 754
             LY+RA + V +GEQ F TVFNVITSMLWGGT+EG++K A A  FR +VS MTELAGKP
Sbjct: 167 AQLYQRAESTVKIGEQVFFTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKP 226

Query: 755 NISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
           NISDFFPS A  D+QGIEK++LKL+PKLDTI
Sbjct: 227 NISDFFPSLACLDVQGIEKKMLKLLPKLDTI 257

HSP 2 Score: 788.1 bits (2034), Expect = 9.9e-225
Identity = 392/522 (75.10%), Postives = 442/522 (84.67%), Query Frame = 1

Query: 3   PTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLGNL 62
           PTSNF              +Q+ F  T   A L+ LY++    R  LPPGP GVPLLGNL
Sbjct: 4   PTSNFFSF-----------DQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNL 63

Query: 63  PFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAG 122
           PFL PELHTYF +LG+KYGPIVKLQLG KIGII+NSPSV RE+LKDHDV FANRDVPQAG
Sbjct: 64  PFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAG 123

Query: 123 RIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGS 182
           R A+YGG DI WTPYGP+WRMLRK+C +KMLSNA LDSVYELR +EV+NTVA+LY RAG+
Sbjct: 124 RAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGT 183

Query: 183 AVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPS 242
           AVNVGEQ FLTVFNVVTSMLWGGSV+G++ RDGLAAEFR+T+S++T LLG PNVSDFFPS
Sbjct: 184 AVNVGEQGFLTVFNVVTSMLWGGSVEGEQ-RDGLAAEFRETVSEMTELLGLPNVSDFFPS 243

Query: 243 LARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGA--KKNDFLQFLLKVKDEGD 302
           LARFD QGIEK+M +L  + D+IFEKMID+RLK+   DEG   KKNDFLQFLL+VKDE +
Sbjct: 244 LARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEE 303

Query: 303 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIV 362
           SKTPLTM  LK LLMDMVIGGTDTSSNT+EFAMAEM+K+PK L+KA+EE+  VVGE NIV
Sbjct: 304 SKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVGEDNIV 363

Query: 363 EESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRD 422
           EESHI SLPYLKA+MKETLRLHPILPLLVPH PS+T ++S YTIPKGSRVF NVWAI RD
Sbjct: 364 EESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQRD 423

Query: 423 PNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSF 482
           P NW+NPLEF PERF N +FDFSG+DFRY PFGSGRRNCAGIAMAERMVMY+LATLLHSF
Sbjct: 424 PKNWDNPLEFDPERFLNGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLATLLHSF 483

Query: 483 DWKLNEVE-KIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           DWKL E + KIE+EEKFGIVLKMK PLVLIP P+L+DPTLYQ
Sbjct: 484 DWKLEEGDGKIEVEEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

BLAST of Cla011547 vs. TrEMBL
Match: A0A0A0KR55_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G159600 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 4.3e-95
Identity = 168/211 (79.62%), Postives = 190/211 (90.05%), Query Frame = 1

Query: 575 RGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITF 634
           RGVP+LGNLPFL PELHTYF +LG+KYGPIVKLQLG KIGII+NSP+V REVLKDHD+TF
Sbjct: 44  RGVPLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTF 103

Query: 635 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV 694
           ANRDVPQAGR A+YGG DI WTPYGP+WRMLRKVC +KMLSNA+LDSVYELRR+EVRNTV
Sbjct: 104 ANRDVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTV 163

Query: 695 GHLYRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 754
            HLY RAG AVNVGEQGFLTVFNV+TSMLWGG+VEG+Q+D  AAEFR  VS+MTEL G P
Sbjct: 164 AHLYARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLP 223

Query: 755 NISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
           N+SDFFPS ARFDLQGIEK++ +L P+ D+I
Sbjct: 224 NVSDFFPSLARFDLQGIEKKMRELAPRFDSI 254

HSP 2 Score: 731.1 bits (1886), Expect = 1.4e-207
Identity = 379/710 (53.38%), Postives = 489/710 (68.87%), Query Frame = 1

Query: 101 VAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDS 160
           +ARE+LKD DV FANRDVP       YGG DI +TPYG EWRMLRK+C   ML +A LD+
Sbjct: 1   MAREVLKDQDVTFANRDVPVVVTAMEYGGRDIVFTPYGAEWRMLRKVCVRDMLGHANLDA 60

Query: 161 VYELRHREVKNTVAYLYRRAGSAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEF 220
           VY  R +E+ NT+ YLY R  S VNVGE +FL V NV+T+MLWGG++ G K R  + AEF
Sbjct: 61  VYSYRRQEIHNTIKYLYSRKDSPVNVGEVMFLNVLNVITNMLWGGTIQG-KERTNIGAEF 120

Query: 221 RQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVD 280
           RQ ++++T LLGKPNVSDFFP LA  D QG +KQM  +  KL+ IF+K+ID+R ++ D  
Sbjct: 121 RQVVAEVTKLLGKPNVSDFFPWLAWLDLQGAKKQMKVVTSKLENIFDKIIDQRTRI-DGQ 180

Query: 281 EG-------AKKNDFLQFLLKVKDEGDSKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAM 340
           +G        +  DFLQ LL++KD GD+KTPLTM  +K LLMDMV+GGT+T+S+T+EFAM
Sbjct: 181 KGIGSSNGNTESKDFLQVLLRLKDAGDAKTPLTMDHVKALLMDMVVGGTETASSTVEFAM 240

Query: 341 AEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLRLHPILPLLVPHSP 400
           AEMM  P++++K + EL  VVG+  IVEE HIQ LPYL A+MKE LRLHP+LPL+V HSP
Sbjct: 241 AEMMNKPEIMKKVQTELENVVGKNAIVEEFHIQKLPYLYAVMKEALRLHPVLPLMVNHSP 300

Query: 401 SQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFFNDQFDFSGSDFRYLPFG 460
           S ++V++ Y IPKG+ VF NVWAIHRDP+ W++PLEF+PERF N + D+SG+DF YLPFG
Sbjct: 301 SVSSVVANYRIPKGAHVFVNVWAIHRDPSIWQSPLEFLPERFLNGKGDYSGNDFNYLPFG 360

Query: 461 SGRRNCAGIAMAERMVMYLLATLLHSFDWKLNEVEKIEIEEKFGIVLKMKKPLVLIPKPR 520
           SGRR CAG+AMAE+MV++ LA+LLHSF+W L   EK+EI EKFGIVLK K PL+ IP PR
Sbjct: 361 SGRRICAGMAMAEKMVLFSLASLLHSFNWTLPAGEKLEISEKFGIVLKKKTPLIAIPTPR 420

Query: 521 LSDPT-----LYQQSLCLIPKILFLSPFHHGTHFQIPSNSYPL--SSHLLRRVSTNFSL- 580
           L+DP      LY  SL  I  ++  +       +   SN   +   + L   ++T F   
Sbjct: 421 LTDPACYDTFLYNTSLLDILNMIMHTILAKCAWWLENSNKRDMLVQAALTVSIATIFLFW 480

Query: 581 C----------QTLAAALARGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGI 640
           C          + L     RG+PV+G LPFL   LHT F EL  +YGPI KLQLG K+ +
Sbjct: 481 CMVAFIKSRRGKVLLPPGPRGLPVVGYLPFLGTNLHTEFAELAHQYGPIFKLQLGNKLCV 540

Query: 641 ILNSPTVAREVLKDHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLS 700
           +L+SP + RE+ +D D+ FANRD P A   ATYGG DI W+PYG  WR +RK+   +MLS
Sbjct: 541 VLSSPPLIREITRDQDVVFANRDPPIAAVAATYGGLDIAWSPYGAYWRNMRKMFVREMLS 600

Query: 701 NASLDSVYELRRREVRNTVGHLYRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDA 760
             +LD+ Y+LR+ EVR  V ++  + G  V++GE  FLT   VI SMLWGGT++ +++  
Sbjct: 601 GRNLDACYDLRKSEVRKAVEYVSTKVGTVVDIGELIFLTEVKVIMSMLWGGTLDAEKQSK 660

Query: 761 FAAEFRGLVSDMTELAGKPNISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
              EFRG V  +     KPNISDFFP  ARFD+QGIEKQV  L+ K+D I
Sbjct: 661 VGPEFRGAVEQIVGTIAKPNISDFFPILARFDIQGIEKQVKALLKKVDEI 708

BLAST of Cla011547 vs. TrEMBL
Match: A0A068UNR1_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00030376001 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 1.7e-136
Identity = 240/498 (48.19%), Postives = 345/498 (69.28%), Query Frame = 1

Query: 27  LLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIVKL 86
           + T+F  + ++ ++K    +  LPPGP G+P++G LPFL   LHT F EL  +YGPI KL
Sbjct: 471 IATIFLFWCMVAFIKSRRGKVLLPPGPRGLPVVGYLPFLGTNLHTEFAELAHQYGPIFKL 530

Query: 87  QLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGFDISWTPYGPEWRMLRK 146
           QLG K+ ++++SP + REI +D DV+FANRD P A   ATYGG DI+W+PYG  WR +RK
Sbjct: 531 QLGNKLCVVLSSPPLIREITRDQDVVFANRDPPIAAVAATYGGLDIAWSPYGAYWRNMRK 590

Query: 147 ICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGSAVNVGEQVFLTVFNVVTSMLWGGS 206
           +   +MLS   LD+ Y+LR  EV+  V Y+  + G+ V++GE +FLT   V+ SMLWGG+
Sbjct: 591 MFVREMLSGRNLDACYDLRKSEVRKAVEYVSTKVGTVVDIGELIFLTEVKVIMSMLWGGT 650

Query: 207 VDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKLDTIF 266
           +D +K +  +  EFR  +  I G + KPN+SDFFP LARFD QGIEKQ+  L+ K+D I 
Sbjct: 651 LDAEK-QSKVGPEFRGAVEQIVGTIAKPNISDFFPILARFDIQGIEKQVKALLKKVDEIV 710

Query: 267 EKMIDERLKMT-----DVDEGAKKNDFLQFLL---KVKDEGDSKTPLTMVQLKGLLMDMV 326
           + +IDER+KM      D      + DF+Q LL   K+++EG+    +T+ Q+K ++MD++
Sbjct: 711 DAVIDERIKMVPNNVEDPIRNEAEKDFIQILLELVKLENEGEK---ITLTQVKAIVMDIM 770

Query: 327 IGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKET 386
           +GGTDT++   E+ M E++ + +++EK ++EL  V+G  N VEE H+  L YL A++KET
Sbjct: 771 VGGTDTTATMAEWVMTEVLHDREIMEKVQKELEDVIGINNTVEEIHLPKLRYLDAVVKET 830

Query: 387 LRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFF-- 446
            RLHP LPLLVP  PSQ+  +  +TIPK +RVF N W IHRDP  W+NPLEF PER    
Sbjct: 831 FRLHPALPLLVPKRPSQSCTVGGFTIPKDARVFVNAWQIHRDPELWDNPLEFKPERILAT 890

Query: 447 NDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLNEVEKIEIEEKF 506
             ++D+SG++F+Y+PFGSGRR CAGI +AE+M+MY+LA+LLHS+DWK  + ++ ++ EKF
Sbjct: 891 PSKWDYSGNNFQYIPFGSGRRICAGIPLAEKMLMYILASLLHSYDWKSTKSKEADLSEKF 950

Query: 507 GIVLKMKKPLVLIPKPRL 515
           GIV++   PL+ IP  +L
Sbjct: 951 GIVMRKSTPLLAIPTKKL 964

HSP 2 Score: 699.9 bits (1805), Expect = 3.5e-198
Identity = 358/758 (47.23%), Postives = 501/758 (66.09%), Query Frame = 1

Query: 30  LFTAFL--LLLYVKYWPRRFR-LPPGPPGVPLLGNLPFLDPELHTYFTELGKKYGPIVKL 89
           L+ A L  ++LY+    RR R LPPGP G+PL+G+LPF+DP LHTYF  L  K+GPI+ +
Sbjct: 7   LYAALLVPMVLYLAVTRRRSRRLPPGPAGLPLVGSLPFIDPNLHTYFAGLAAKHGPILSI 66

Query: 90  QLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGF-DISWTPYGPEWRMLR 149
           +LG K+ I+V SP +ARE+L+D D +FANR +P AG   ++GG  +I   P GP WR+LR
Sbjct: 67  RLGSKVDIVVTSPELAREVLRDQDSVFANRVMPDAGNAVSFGGVQNIVGNPVGPMWRLLR 126

Query: 150 KICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGSAVNVGEQVFLTVFNVVTSMLWGG 209
           ++C  +MLS +GL +V++LR RE ++T+ YL+ ++G  V++G Q+FL   NV+TS +WGG
Sbjct: 127 RLCVHEMLSPSGLANVHDLRRREFRSTLRYLHTKSGEPVDLGAQMFLNTMNVITSAMWGG 186

Query: 210 SVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQGIEKQMLKLVLKLDTI 269
           ++  +  R  + +EFR  ++++T LLG PNVSDFFP+LA FD QGI  +M +   + D +
Sbjct: 187 TIGSESERAAVGSEFRGLVAEVTELLGTPNVSDFFPALAPFDLQGIRGKMERFRSRFDLM 246

Query: 270 FEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEG-DSKTPLTMVQLKGLLMDMVIGGTDT 329
           F K+I +R+K    D G    DFL+ +LK++ EG D K P TM  +K LL+DMV+GGT+T
Sbjct: 247 FAKIIQQRMKAQQ-DGGETATDFLECMLKMEKEGGDGKAPFTMDNVKALLLDMVVGGTET 306

Query: 330 SSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLPYLKAIMKETLRLHPI 389
           +SNT+E+AMAEM++NP+VL+KA++EL  VVG   +VEE H+  L YL+ ++KETLRLHP 
Sbjct: 307 TSNTVEWAMAEMLQNPQVLKKAQQELDSVVGRDEVVEEWHLPQLHYLRMVIKETLRLHPA 366

Query: 390 LPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLEFVPERFFNDQ---FD 449
           LPL+VPHSPS  + +  Y +P+GSRVF NVWAI R+P  W  PLEF PERF  D+   +D
Sbjct: 367 LPLMVPHSPSAASTVGGYHVPEGSRVFVNVWAIQRNPLVWNEPLEFNPERFAGDEGHKWD 426

Query: 450 FSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLNEVEKIEIEEKFGIVLK 509
           F+GS F Y PFGSGRR CAGIAMAE+M  Y +A LL +FDWKL +  + ++ EKFGIV+K
Sbjct: 427 FTGSQFDYFPFGSGRRICAGIAMAEKMTAYSVAMLLQAFDWKLPQGVQPDLSEKFGIVMK 486

Query: 510 MKKPLVLIPKPRLSDPTLYQQSLCLIPKILFLSPFHHGTHFQIPSNSYPLSSHLLRRVST 569
              PLV IP P LS   LY            ++   H     +P                
Sbjct: 487 KAAPLVAIPTPSLSSAFLY------------ITIVRHRRGGDLPPG-------------- 546

Query: 570 NFSLCQTLAAALARGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPT 629
                         G+P++G LPFL+P LH YF  L  K+GP+  +++G K+ +++ SP 
Sbjct: 547 ------------PAGLPLIGCLPFLEPNLHAYFARLAEKHGPVFSIRIGSKLEVVVTSPE 606

Query: 630 VAREVLKDHDITFANRDVPQAGRVATYGGFD-ITWTPYGPEWRMLRKVCTIKMLSNASLD 689
           VAREVL+D D  FANR +P+AGR   +G  D I   P GP+ R+LR++C  +ML  + L 
Sbjct: 607 VAREVLRDQDHVFANRVIPEAGRAIAFGEEDNIVGNPAGPKLRLLRRICVSEMLGPSGLS 666

Query: 690 SVYELRRREVRNTVGHLYRRA--GAAVNVGEQGFLTVFNVITSMLWGGTVEGD-QKDAFA 749
            +Y LRRRE R+T+ HLY ++  G  V VG Q FLT  N IT+ LWGG +  + ++ A  
Sbjct: 667 KMYGLRRREFRSTLRHLYMQSQCGTPVCVGAQMFLTAMNTITNSLWGGKIGSESERTAVG 725

Query: 750 AEFRGLVSDMTELAGKPNISDFFPSFARFDLQGIEKQV 776
            EFR L++++TEL G+PN+SDFFP  A  DLQG++K++
Sbjct: 727 QEFRALIAEITELLGRPNVSDFFPVLAPLDLQGVQKRM 725

BLAST of Cla011547 vs. NCBI nr
Match: gi|449459696|ref|XP_004147582.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 877.1 bits (2265), Expect = 2.3e-251
Identity = 432/521 (82.92%), Postives = 467/521 (89.64%), Query Frame = 1

Query: 1   MEPTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLG 60
           M  TSNFLHSL    +F DPNN+T FLLTLF  FLLLLYVK+  RR  LPPGPPGVPLLG
Sbjct: 1   MALTSNFLHSL----DFFDPNNKTHFLLTLFAGFLLLLYVKFSRRRIHLPPGPPGVPLLG 60

Query: 61  NLPFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQ 120
           NLPFLDPELH+YFTELG+KYGPIV+LQLGGKIGIIVNS S+ARE+LKDHD+ FAN DV +
Sbjct: 61  NLPFLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHE 120

Query: 121 AGRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRA 180
           AGR ATYGG DISW PYGPEWRMLRK+CTIKMLSNA LDSVYELR REV+NTVA+LYRRA
Sbjct: 121 AGRAATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRA 180

Query: 181 GSAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFF 240
           GSAVNVGEQ FLT+FNVVTSMLWGGSV+ D+ RD +AAEFR+TIS+IT LLG+PNVSDFF
Sbjct: 181 GSAVNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFF 240

Query: 241 PSLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEGD 300
           PSLARFD QGIEKQM+K VLKLDTIFEKMIDER++M DV+E  KKNDFLQFLL VKDEGD
Sbjct: 241 PSLARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNESVKKNDFLQFLLTVKDEGD 300

Query: 301 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIV 360
           SKTPLTMVQLKGLLMDMV GGTDTSSNT+EFAM EMMKNPK+LEKAKEEL  VVGEQ +V
Sbjct: 301 SKTPLTMVQLKGLLMDMVTGGTDTSSNTVEFAMGEMMKNPKILEKAKEELCAVVGEQRMV 360

Query: 361 EESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRD 420
           EESHIQSL YLKA+MKETLRLHPILPLL+PH PS+TTV+S YTIPKGSRVF NVWAI RD
Sbjct: 361 EESHIQSLSYLKAVMKETLRLHPILPLLIPHCPSETTVVSNYTIPKGSRVFVNVWAIQRD 420

Query: 421 PNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSF 480
           PN WENPL F PERF N +FDFSGSDFRY PFGSGRRNCAGIAMAER VMYLLATLLHSF
Sbjct: 421 PNEWENPLVFDPERFLNGKFDFSGSDFRYFPFGSGRRNCAGIAMAERTVMYLLATLLHSF 480

Query: 481 DWKLNEVEKIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           DWKL E EKIE+EEKFGIVLKMKKPLVLIP+PRL DPTLY+
Sbjct: 481 DWKLEEGEKIEVEEKFGIVLKMKKPLVLIPRPRLLDPTLYE 517

BLAST of Cla011547 vs. NCBI nr
Match: gi|449459696|ref|XP_004147582.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 359.0 bits (920), Expect = 2.1e-95
Identity = 184/261 (70.50%), Postives = 210/261 (80.46%), Query Frame = 1

Query: 534 SPFHHGTHFQIPSNSYPLSSHLLRRVSTNFSLCQTLAAALAR--------GVPVLGNLPF 593
           S F H   F  P+N     +H L  +   F L   +  +  R        GVP+LGNLPF
Sbjct: 5   SNFLHSLDFFDPNNK----THFLLTLFAGFLLLLYVKFSRRRIHLPPGPPGVPLLGNLPF 64

Query: 594 LDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFANRDVPQAGRV 653
           LDPELH+YFTELGRKYGPIV+LQLGGKIGII+NS ++AREVLKDHDITFAN DV +AGR 
Sbjct: 65  LDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGRA 124

Query: 654 ATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGHLYRRAGAAV 713
           ATYGG DI+W PYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV HLYRRAG+AV
Sbjct: 125 ATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSAV 184

Query: 714 NVGEQGFLTVFNVITSMLWGGTVEGDQ-KDAFAAEFRGLVSDMTELAGKPNISDFFPSFA 773
           NVGEQGFLT+FNV+TSMLWGG+VE DQ +D  AAEFR  +S++T+L G+PN+SDFFPS A
Sbjct: 185 NVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSLA 244

Query: 774 RFDLQGIEKQVLKLVPKLDTI 786
           RFDLQGIEKQ++K V KLDTI
Sbjct: 245 RFDLQGIEKQMVKHVLKLDTI 261

HSP 2 Score: 867.5 bits (2240), Expect = 1.8e-248
Identity = 430/521 (82.53%), Postives = 464/521 (89.06%), Query Frame = 1

Query: 1   MEPTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLG 60
           ME TSNFL + IHSLNF     +T FL TLF  FLLLLYVK   RR  LPPGP GVPLLG
Sbjct: 1   MELTSNFLQTFIHSLNFY----KTHFLFTLFAGFLLLLYVKLSQRRIHLPPGPTGVPLLG 60

Query: 61  NLPFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQ 120
           NLPFLDPELH+YFTELG+KYGPIV+L+LGGKIGIIVNS SVARE+LKDHD+ FANRDVPQ
Sbjct: 61  NLPFLDPELHSYFTELGRKYGPIVRLRLGGKIGIIVNSSSVAREVLKDHDITFANRDVPQ 120

Query: 121 AGRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRA 180
           AGR ATYGG+DISW PYGPEWRMLRK+CT+KMLSNA LDSVYELR REV+ TVA+LYRRA
Sbjct: 121 AGRAATYGGYDISWAPYGPEWRMLRKVCTVKMLSNASLDSVYELRRREVRKTVAHLYRRA 180

Query: 181 GSAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFF 240
           GSAVNVGEQ FLT+FNVVTSMLWGGSV+ D+ RD +AA+FR+T+SD+T LLG PNVSDFF
Sbjct: 181 GSAVNVGEQGFLTIFNVVTSMLWGGSVENDQSRDDIAADFRETVSDVTKLLGLPNVSDFF 240

Query: 241 PSLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGAKKNDFLQFLLKVKDEGD 300
           PSLARFD QGIEKQM K V KLD IFEKMIDERL+M DV+E AKK+DFLQFLLKVKDEGD
Sbjct: 241 PSLARFDLQGIEKQMGKHVQKLDAIFEKMIDERLRMKDVNESAKKDDFLQFLLKVKDEGD 300

Query: 301 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIV 360
           SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAE+MKNPK++EKAKEEL  VVGEQ++V
Sbjct: 301 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEIMKNPKIVEKAKEELCAVVGEQSMV 360

Query: 361 EESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRD 420
           EESHIQSLPYLKA+MKE LRLHP LPLLVPH PS+TTVIS YTIPKGSR+F N+WAI RD
Sbjct: 361 EESHIQSLPYLKAVMKEALRLHPTLPLLVPHCPSETTVISNYTIPKGSRMFVNIWAIQRD 420

Query: 421 PNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSF 480
           PN WENP  F PERF N +FDFSGSDFRYLPFGSGRRNCAGIAMAER VMYLLATLLHSF
Sbjct: 421 PNEWENPSVFDPERFLNGKFDFSGSDFRYLPFGSGRRNCAGIAMAERTVMYLLATLLHSF 480

Query: 481 DWKLNEVEKIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           DWKL E EKIEIEEKFGIVLKMKKPLVLIP PRL DPTLYQ
Sbjct: 481 DWKLEEGEKIEIEEKFGIVLKMKKPLVLIPTPRLLDPTLYQ 517

BLAST of Cla011547 vs. NCBI nr
Match: gi|659073578|ref|XP_008437137.1| (PREDICTED: geraniol 8-hydroxylase-like [Cucumis melo])

HSP 1 Score: 360.9 bits (925), Expect = 5.6e-96
Identity = 182/255 (71.37%), Postives = 206/255 (80.78%), Query Frame = 1

Query: 532 FLSPFHHGTHFQIPSNSYPLSSHLLRRVSTNFSLCQTLAAALARGVPVLGNLPFLDPELH 591
           FL  F H  +F      + L +  L  +    S  +        GVP+LGNLPFLDPELH
Sbjct: 7   FLQTFIHSLNFYKTHFLFTLFAGFLLLLYVKLSQRRIHLPPGPTGVPLLGNLPFLDPELH 66

Query: 592 TYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITFANRDVPQAGRVATYGGF 651
           +YFTELGRKYGPIV+L+LGGKIGII+NS +VAREVLKDHDITFANRDVPQAGR ATYGG+
Sbjct: 67  SYFTELGRKYGPIVRLRLGGKIGIIVNSSSVAREVLKDHDITFANRDVPQAGRAATYGGY 126

Query: 652 DITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVGHLYRRAGAAVNVGEQG 711
           DI+W PYGPEWRMLRKVCT+KMLSNASLDSVYELRRREVR TV HLYRRAG+AVNVGEQG
Sbjct: 127 DISWAPYGPEWRMLRKVCTVKMLSNASLDSVYELRRREVRKTVAHLYRRAGSAVNVGEQG 186

Query: 712 FLTVFNVITSMLWGGTVEGDQ-KDAFAAEFRGLVSDMTELAGKPNISDFFPSFARFDLQG 771
           FLT+FNV+TSMLWGG+VE DQ +D  AA+FR  VSD+T+L G PN+SDFFPS ARFDLQG
Sbjct: 187 FLTIFNVVTSMLWGGSVENDQSRDDIAADFRETVSDVTKLLGLPNVSDFFPSLARFDLQG 246

Query: 772 IEKQVLKLVPKLDTI 786
           IEKQ+ K V KLD I
Sbjct: 247 IEKQMGKHVQKLDAI 261

HSP 2 Score: 808.1 bits (2086), Expect = 1.3e-230
Identity = 403/512 (78.71%), Postives = 444/512 (86.72%), Query Frame = 1

Query: 12  IHSLNFIDPNNQTRFLLTLFTAFLLL-LYVKYWPRRFRLPPGPPGVPLLGNLPFLDPELH 71
           + +LN  +PNNQT FLL LF  FLL+ LYVK    R +LPPGP GVPLLGNLPFLDP LH
Sbjct: 4   LQTLNSYNPNNQTGFLLALFAGFLLIFLYVKLSQPRLQLPPGPRGVPLLGNLPFLDPNLH 63

Query: 72  TYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAGRIATYGGF 131
           TYF ELG+KYG IVKLQLGGK+GIIVNSPSVAREILKDHD+ FANRDVPQAGR+ATYGGF
Sbjct: 64  TYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGGF 123

Query: 132 DISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGSAVNVGEQV 191
           DI+WTPYGPEWRMLRK+CTIK+L NA LD VYELR  EV+ TVA LY+RA S V +GEQV
Sbjct: 124 DITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQV 183

Query: 192 FLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPSLARFDFQG 251
           F TVFNV+TSMLWGG+++G++ +  +A  FR+ +S +T L GKPN+SDFFPSLA  D QG
Sbjct: 184 FFTVFNVITSMLWGGTMEGEE-KAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQG 243

Query: 252 IEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGAK-KNDFLQFLLKVKDEGDSKTPLTMVQ 311
           IEK+MLKL+ KLDTIFEK+IDERL+MT+ +EG++ KNDFLQFLLKVKDE DS+TPLT+VQ
Sbjct: 244 IEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVVQ 303

Query: 312 LKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIVEESHIQSLP 371
           LK LLMDMV GGTDTSSNTIEFAMAEMMKNPKV EKAKEEL  VVGEQ+IVEESHIQSLP
Sbjct: 304 LKALLMDMVFGGTDTSSNTIEFAMAEMMKNPKVAEKAKEELRAVVGEQSIVEESHIQSLP 363

Query: 372 YLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRDPNNWENPLE 431
           YLKAIMKETLRLHPILPLLVPH PS TTV+S YTIPKGSRVF NVWAI RDPN WENPLE
Sbjct: 364 YLKAIMKETLRLHPILPLLVPHCPSDTTVVSNYTIPKGSRVFVNVWAIQRDPNEWENPLE 423

Query: 432 FVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSFDWKLNEVEK 491
           F PERF N + DFSGSDFRY PFGSGRR C GIAM ERMVMYLLATLLHSFDWKL E E+
Sbjct: 424 FDPERFMNGKLDFSGSDFRYFPFGSGRRKCPGIAMGERMVMYLLATLLHSFDWKLEEGEE 483

Query: 492 IEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           IEIEEKFGIVL MKKPLVLIP PRLSDPTLYQ
Sbjct: 484 IEIEEKFGIVLTMKKPLVLIPTPRLSDPTLYQ 514

BLAST of Cla011547 vs. NCBI nr
Match: gi|778699925|ref|XP_011654786.1| (PREDICTED: flavonoid 3',5'-hydroxylase-like [Cucumis sativus])

HSP 1 Score: 351.7 bits (901), Expect = 3.4e-93
Identity = 170/211 (80.57%), Postives = 188/211 (89.10%), Query Frame = 1

Query: 575 RGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITF 634
           RGVP+LGNLPFLDP LHTYF ELG+KYG IVKLQLGGK+GII+NSP+VARE+LKDHDITF
Sbjct: 47  RGVPLLGNLPFLDPNLHTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITF 106

Query: 635 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV 694
           ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIK+L NASLD VYELRR EVR TV
Sbjct: 107 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTV 166

Query: 695 GHLYRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 754
             LY+RA + V +GEQ F TVFNVITSMLWGGT+EG++K A A  FR +VS MTELAGKP
Sbjct: 167 AQLYQRAESTVKIGEQVFFTVFNVITSMLWGGTMEGEEKAAVAVVFREMVSAMTELAGKP 226

Query: 755 NISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
           NISDFFPS A  D+QGIEK++LKL+PKLDTI
Sbjct: 227 NISDFFPSLACLDVQGIEKKMLKLLPKLDTI 257

HSP 2 Score: 788.1 bits (2034), Expect = 1.4e-224
Identity = 392/522 (75.10%), Postives = 442/522 (84.67%), Query Frame = 1

Query: 3   PTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLGNL 62
           PTSNF              +Q+ F  T   A L+ LY++    R  LPPGP GVPLLGNL
Sbjct: 4   PTSNFFSF-----------DQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGVPLLGNL 63

Query: 63  PFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQAG 122
           PFL PELHTYF +LG+KYGPIVKLQLG KIGII+NSPSV RE+LKDHDV FANRDVPQAG
Sbjct: 64  PFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPQAG 123

Query: 123 RIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAGS 182
           R A+YGG DI WTPYGP+WRMLRK+C +KMLSNA LDSVYELR +EV+NTVA+LY RAG+
Sbjct: 124 RAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTVAHLYARAGT 183

Query: 183 AVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFPS 242
           AVNVGEQ FLTVFNVVTSMLWGGSV+G++ RDGLAAEFR+T+S++T LLG PNVSDFFPS
Sbjct: 184 AVNVGEQGFLTVFNVVTSMLWGGSVEGEQ-RDGLAAEFRETVSEMTELLGLPNVSDFFPS 243

Query: 243 LARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEGA--KKNDFLQFLLKVKDEGD 302
           LARFD QGIEK+M +L  + D+IFEKMID+RLK+   DEG   KKNDFLQFLL+VKDE +
Sbjct: 244 LARFDLQGIEKKMRELAPRFDSIFEKMIDQRLKIDGGDEGGSVKKNDFLQFLLQVKDEEE 303

Query: 303 SKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNIV 362
           SKTPLTM  LK LLMDMVIGGTDTSSNT+EFAMAEM+K+PK L+KA+EE+  VVGE NIV
Sbjct: 304 SKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQEEVVAVVGEDNIV 363

Query: 363 EESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHRD 422
           EESHI SLPYLKA+MKETLRLHPILPLLVPH PS+T ++S YTIPKGSRVF NVWAI RD
Sbjct: 364 EESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQRD 423

Query: 423 PNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHSF 482
           P NW+NPLEF PERF N +FDFSG+DFRY PFGSGRRNCAGIAMAERMVMY+LATLLHSF
Sbjct: 424 PKNWDNPLEFDPERFLNGKFDFSGNDFRYFPFGSGRRNCAGIAMAERMVMYMLATLLHSF 483

Query: 483 DWKLNEVE-KIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           DWKL E + KIE+EEKFGIVLKMK PLVLIP P+L+DPTLYQ
Sbjct: 484 DWKLEEGDGKIEVEEKFGIVLKMKTPLVLIPTPKLADPTLYQ 513

BLAST of Cla011547 vs. NCBI nr
Match: gi|449459692|ref|XP_004147580.1| (PREDICTED: flavonoid 3'-monooxygenase-like [Cucumis sativus])

HSP 1 Score: 357.5 bits (916), Expect = 6.2e-95
Identity = 168/211 (79.62%), Postives = 190/211 (90.05%), Query Frame = 1

Query: 575 RGVPVLGNLPFLDPELHTYFTELGRKYGPIVKLQLGGKIGIILNSPTVAREVLKDHDITF 634
           RGVP+LGNLPFL PELHTYF +LG+KYGPIVKLQLG KIGII+NSP+V REVLKDHD+TF
Sbjct: 44  RGVPLLGNLPFLHPELHTYFAQLGQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTF 103

Query: 635 ANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTV 694
           ANRDVPQAGR A+YGG DI WTPYGP+WRMLRKVC +KMLSNA+LDSVYELRR+EVRNTV
Sbjct: 104 ANRDVPQAGRAASYGGSDIVWTPYGPKWRMLRKVCVVKMLSNATLDSVYELRRKEVRNTV 163

Query: 695 GHLYRRAGAAVNVGEQGFLTVFNVITSMLWGGTVEGDQKDAFAAEFRGLVSDMTELAGKP 754
            HLY RAG AVNVGEQGFLTVFNV+TSMLWGG+VEG+Q+D  AAEFR  VS+MTEL G P
Sbjct: 164 AHLYARAGTAVNVGEQGFLTVFNVVTSMLWGGSVEGEQRDGLAAEFRETVSEMTELLGLP 223

Query: 755 NISDFFPSFARFDLQGIEKQVLKLVPKLDTI 786
           N+SDFFPS ARFDLQGIEK++ +L P+ D+I
Sbjct: 224 NVSDFFPSLARFDLQGIEKKMRELAPRFDSI 254

HSP 2 Score: 786.9 bits (2031), Expect = 3.2e-224
Identity = 390/523 (74.57%), Postives = 441/523 (84.32%), Query Frame = 1

Query: 2   EPTSNFLHSLIHSLNFIDPNNQTRFLLTLFTAFLLLLYVKYWPRRFRLPPGPPGVPLLGN 61
           +PTSNF              +Q+ F  T   A L+ LY++    R  LPPGP GVPLLGN
Sbjct: 3   DPTSNFFSI-----------DQSHFFFTFLAALLIFLYLRLTRLRVPLPPGPRGVPLLGN 62

Query: 62  LPFLDPELHTYFTELGKKYGPIVKLQLGGKIGIIVNSPSVAREILKDHDVIFANRDVPQA 121
           LPFLDPELHTYF +L +KYGPIVKLQLG KIGII+NSPSV RE+LKDHDV FANRDVP A
Sbjct: 63  LPFLDPELHTYFAQLSQKYGPIVKLQLGRKIGIIINSPSVVREVLKDHDVTFANRDVPHA 122

Query: 122 GRIATYGGFDISWTPYGPEWRMLRKICTIKMLSNAGLDSVYELRHREVKNTVAYLYRRAG 181
           GR A+YGG DI WTPYGP+WRMLRK+C +KMLSNA LDSVYELR REV+NTVA+LY RAG
Sbjct: 123 GRAASYGGSDIVWTPYGPQWRMLRKVCVLKMLSNATLDSVYELRRREVRNTVAHLYARAG 182

Query: 182 SAVNVGEQVFLTVFNVVTSMLWGGSVDGDKIRDGLAAEFRQTISDITGLLGKPNVSDFFP 241
           + VNVGEQ FLTVFNVVTSMLWGGSV+G++ RDGLAAEFR+T+S++T LLG PNVSDFFP
Sbjct: 183 TRVNVGEQGFLTVFNVVTSMLWGGSVEGEQ-RDGLAAEFRETVSEMTELLGLPNVSDFFP 242

Query: 242 SLARFDFQGIEKQMLKLVLKLDTIFEKMIDERLKMTDVDEG--AKKNDFLQFLLKVKDEG 301
           SLARFD QGIEK+M +L  + D+IFEKMID+RLK+   DEG   KKNDFLQFLL+VKD+G
Sbjct: 243 SLARFDIQGIEKKMRELAPRFDSIFEKMIDQRLKIDGKDEGESVKKNDFLQFLLQVKDDG 302

Query: 302 DSKTPLTMVQLKGLLMDMVIGGTDTSSNTIEFAMAEMMKNPKVLEKAKEELAIVVGEQNI 361
           +SKTPLTM  LK LLMDMVIGGTDTSSNT+EFAMAEM+K+PK L+KA++EL  VVGE NI
Sbjct: 303 ESKTPLTMTHLKALLMDMVIGGTDTSSNTVEFAMAEMLKSPKTLKKAQQELVAVVGEDNI 362

Query: 362 VEESHIQSLPYLKAIMKETLRLHPILPLLVPHSPSQTTVISKYTIPKGSRVFFNVWAIHR 421
           VEESHI SLPYLKA+MKETLRLHPILPLLVPH PS+T ++S YTIPKGSRVF NVWAI R
Sbjct: 363 VEESHIHSLPYLKAVMKETLRLHPILPLLVPHCPSETAIVSNYTIPKGSRVFINVWAIQR 422

Query: 422 DPNNWENPLEFVPERFFNDQFDFSGSDFRYLPFGSGRRNCAGIAMAERMVMYLLATLLHS 481
           DP NW+NPLEF PERF N ++DFSG+DFRY PFGSGRRNCAGI MAERMVMYLLATLLHS
Sbjct: 423 DPKNWDNPLEFDPERFLNGKYDFSGNDFRYFPFGSGRRNCAGIGMAERMVMYLLATLLHS 482

Query: 482 FDWKLNE-VEKIEIEEKFGIVLKMKKPLVLIPKPRLSDPTLYQ 522
           FDWKL +  EKIE+EEKFGIVLKMK PLV+IP P+LSDPTLYQ
Sbjct: 483 FDWKLGDGDEKIEVEEKFGIVLKMKTPLVVIPTPKLSDPTLYQ 513