Cla010684 (gene) Watermelon (97103) v1

NameCla010684
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPolygalacturonase (AHRD V1 **-- A8MRN3_ARATH); contains Interpro domain(s) IPR012334 Pectin lyase fold
LocationChr7 : 28827224 .. 28829211 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTCTTTTTTGTCTTTGTTTTAGTACTTTCCGTTGTCATTTTAAACTCATGGAGTGTATCAGCCTCATCTAATTTTAATGTTCTCCATTATGGAGCTACTGGAAATGGCAAAACTGATGATTCCCAAGTGAGTAATTTACAACTCCAATTTGATATATATATATATATATATATATATATATATTTTGAAGCAATTGTTATTCTCTCTCTAATTTATTTATTTATTTATTTATTTTTTATTTTTTATTATTTTTTTTTTTTGTTTAAAGGCTTTCTTGAGAGCTTGGAAAGATGTATGTGAAGCAACCGAAGGCATTCCAACATTACATGTTCCACTTGCAAAATCTTACCTATTGAACCCTCTCACTTTCGAGGGTCCTTGCAAGTCCAAACAAATCAATATACATGTAATTTATCTCTCACCCTATATTCCTAAAAAAGTTTATAATATTTGTTTGTTTTAATTTTAGCTTTAACCTACTCTTTGATCAAATTACACCTTTAATCTTTGAGGAACCCTTTTAATTAGAAAATGAGGTTATTGTGCATATAAGTCTAATACTATTTTATATTTATTTTTGTAGCTTAGAGGGACTCTTGTAGCTCCAAGCAAGGATGCATGGCCAAGTGACAAAAAAGATAAATGGATCAAATTTTATGATATAGATGGACTTACAATCAGTGGAGGAGGACAAATTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGAAGAACATTGCAAACGACCCACGGTAGCTTTTAATCCATTTCCTTCCTACGCATTGTTAAATTTATAATATAGATATTATTAATTTTATATTATATGGTTTTTCAGGCATTATTTTTTCATAATTGTAATGGACTCCGATTAAGCAATATGAAGCACATCAATAGTGGCAAGAATCACATCTCCATAAATGTTTGCAATGATGTTATTGTCTCAAATATTCACATTACTGCTCCTGATGAGAGTCCTAACACAGATGGAATTGATATTTCTAGATCAAACAATGTTCTTATTAAAAATTCTTTCATGGCAACGGGTAACAAATTTTATTATTTTTCTTTGAGAATATTAGTTTTATTTCTATGGTTTTGATGCATATATTATGATTTTTTTAAAAAAAAAAATTTGTTAATGTAGGTGATGATTGTGTTGCGATTAACAATGGATCTTCCAACATCAATATCGTAGGTGTCACTTGTGGCCCAGGCCATGGCATAAGGTAAGAAGGACCATTTCTAATTAACATATAATATTTCATCTTTTTATTTGTATTTTGTAATTAGATTTGTAGCACTCGATTCCTATATATTTTTGTTTTGTTTATTTGAATGTAGCATTGGGAGCTTAGGAGAAGATAGAAAATACAACACAGTTGAAAATATCTTTGTGAGCAATTGCTTCTTCAAGGGAACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGTGATATGATTAGTTTTCAATTTATTCATATATGTTGCTTTATTTAACATACGTTATAAAAAAGTAAATATGTGTGTTGAATATAATTATAGGGTGGATATGGATATGCAAGAAACATCACATTCGAAAAAATAACACTCACGAATACAAAAAATCCAATCATTATTGATCAATATTACACTGCTTTGGCAAGTGCAAGGAAGAATAAGGTACAACAACATATTAATTTTATGTTATTTTCATCACATTTTGTTCATAGATAATTACATTTATTTTTAATTACAATTTGTTTTCAGGAGTTGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTTATGGAACGTCAGCAAATGAAAATGCAATTATTTTGAACTGTTCGCGAGCTCGTTGCACCAATATTGTAATGAACAATGTGAACATAAAGACATCAACTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAGAATGTTGAAGGAAAAGCCGAATTGGCTGTTCCAACTGTACCTTGCTTATCAAAGTCCGATTAA

mRNA sequence

ATGAAGCTCTTTTTTGTCTTTGTTTTAGTACTTTCCGTTGTCATTTTAAACTCATGGAGTGTATCAGCCTCATCTAATTTTAATGTTCTCCATTATGGAGCTACTGGAAATGGCAAAACTGATGATTCCCAAGCTTTCTTGAGAGCTTGGAAAGATGTATGTGAAGCAACCGAAGGCATTCCAACATTACATGTTCCACTTGCAAAATCTTACCTATTGAACCCTCTCACTTTCGAGGGTCCTTGCAAGTCCAAACAAATCAATATACATCTTAGAGGGACTCTTGTAGCTCCAAGCAAGGATGCATGGCCAAGTGACAAAAAAGATAAATGGATCAAATTTTATGATATAGATGGACTTACAATCAGTGGAGGAGGACAAATTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGAAGAACATTGCAAACGACCCACGGCATTATTTTTTCATAATTGTAATGGACTCCGATTAAGCAATATGAAGCACATCAATAGTGGCAAGAATCACATCTCCATAAATGTTTGCAATGATGTTATTGTCTCAAATATTCACATTACTGCTCCTGATGAGAGTCCTAACACAGATGGAATTGATATTTCTAGATCAAACAATGTTCTTATTAAAAATTCTTTCATGGCAACGGGTGATGATTGTGTTGCGATTAACAATGGATCTTCCAACATCAATATCGTAGGTGTCACTTGTGGCCCAGGCCATGGCATAAGCATTGGGAGCTTAGGAGAAGATAGAAAATACAACACAGTTGAAAATATCTTTGTGAGCAATTGCTTCTTCAAGGGAACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGGTGGATATGGATATGCAAGAAACATCACATTCGAAAAAATAACACTCACGAATACAAAAAATCCAATCATTATTGATCAATATTACACTGCTTTGGCAAGTGCAAGGAAGAATAAGGAGTTGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTTATGGAACGTCAGCAAATGAAAATGCAATTATTTTGAACTGTTCGCGAGCTCGTTGCACCAATATTGTAATGAACAATGTGAACATAAAGACATCAACTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAGAATGTTGAAGGAAAAGCCGAATTGGCTGTTCCAACTGTACCTTGCTTATCAAAGTCCGATTAA

Coding sequence (CDS)

ATGAAGCTCTTTTTTGTCTTTGTTTTAGTACTTTCCGTTGTCATTTTAAACTCATGGAGTGTATCAGCCTCATCTAATTTTAATGTTCTCCATTATGGAGCTACTGGAAATGGCAAAACTGATGATTCCCAAGCTTTCTTGAGAGCTTGGAAAGATGTATGTGAAGCAACCGAAGGCATTCCAACATTACATGTTCCACTTGCAAAATCTTACCTATTGAACCCTCTCACTTTCGAGGGTCCTTGCAAGTCCAAACAAATCAATATACATCTTAGAGGGACTCTTGTAGCTCCAAGCAAGGATGCATGGCCAAGTGACAAAAAAGATAAATGGATCAAATTTTATGATATAGATGGACTTACAATCAGTGGAGGAGGACAAATTGATGGCCAAGGTTCTTCTTGGTGGAAAGATTGTGAAGAACATTGCAAACGACCCACGGCATTATTTTTTCATAATTGTAATGGACTCCGATTAAGCAATATGAAGCACATCAATAGTGGCAAGAATCACATCTCCATAAATGTTTGCAATGATGTTATTGTCTCAAATATTCACATTACTGCTCCTGATGAGAGTCCTAACACAGATGGAATTGATATTTCTAGATCAAACAATGTTCTTATTAAAAATTCTTTCATGGCAACGGGTGATGATTGTGTTGCGATTAACAATGGATCTTCCAACATCAATATCGTAGGTGTCACTTGTGGCCCAGGCCATGGCATAAGCATTGGGAGCTTAGGAGAAGATAGAAAATACAACACAGTTGAAAATATCTTTGTGAGCAATTGCTTCTTCAAGGGAACTCAAAATGGAGTTAGAATCAAGACTTGGGAGGGTGGATATGGATATGCAAGAAACATCACATTCGAAAAAATAACACTCACGAATACAAAAAATCCAATCATTATTGATCAATATTACACTGCTTTGGCAAGTGCAAGGAAGAATAAGGAGTTGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTTATGGAACGTCAGCAAATGAAAATGCAATTATTTTGAACTGTTCGCGAGCTCGTTGCACCAATATTGTAATGAACAATGTGAACATAAAGACATCAACTCCAGGAAAAGAAGCCAAAGCTTTTTGCCAGAATGTTGAAGGAAAAGCCGAATTGGCTGTTCCAACTGTACCTTGCTTATCAAAGTCCGATTAA

Protein sequence

MKLFFVFVLVLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFEGPCKSKQINIHLRGTLVAPSKDAWPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKDCEEHCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRARCTNIVMNNVNIKTSTPGKEAKAFCQNVEGKAELAVPTVPCLSKSD
BLAST of Cla010684 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.1e-103
Identity = 194/379 (51.19%), Postives = 253/379 (66.75%), Query Frame = 1

Query: 22  SASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFEGP 81
           ++S+  +V  +GA G+G TDDSQAFL+AW+ VC  T G     VP   +++L PL F+G 
Sbjct: 19  TSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQGS 78

Query: 82  CKSKQINIHLRGTLVAPSKDAWPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKDCEE 141
           CKS  + + + G LVAPSK  W  DK D+WI F DI+GL I G G+I+GQGSSWW   E 
Sbjct: 79  CKSTPVFVQMLGKLVAPSKGNWKGDK-DQWILFTDIEGLVIEGDGEINGQGSSWW---EH 138

Query: 142 HCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPDESPNTDGIDI 201
              RPTAL F +CN LRLS + H++S   HI I+ CN V +S++ I AP+ SPNTDGID+
Sbjct: 139 KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDV 198

Query: 202 SRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGEDRKYNTVENIF 261
             S+NV+I++  +ATGDDC+AIN+G+SNI+I G+ CGPGHGISIGSLG+D +  TVEN+ 
Sbjct: 199 GASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVC 258

Query: 262 VSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTALAS--ARKNK 321
           V NC F+GT NG RIKTW+GG GYAR ITF  ITL N +NPIIIDQ+Y    S  A+  K
Sbjct: 259 VQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRK 318

Query: 322 ELNIKVSDVTYREVYGTSANENAIILNCS-RARCTNIVMNNVNIKT--STPGKEAKAFCQ 381
              ++VS V +    GTS +E  +   CS R  CT I + ++ I+T  S  G+ A+  C 
Sbjct: 319 SSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCL 378

Query: 382 NVEGKAELAVPTVPCLSKS 396
           NV G + +AVP + CL  S
Sbjct: 379 NVRGASTIAVPGLECLELS 392

BLAST of Cla010684 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.7e-80
Identity = 173/383 (45.17%), Postives = 235/383 (61.36%), Query Frame = 1

Query: 10  VLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAK 69
           VLS++  +  ++ AS+  +V ++GA G+GKTDD+QAF +AWK  C +T G+ T  VP  K
Sbjct: 52  VLSLISSDETTLEAST-VSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGK 111

Query: 70  SYLLNPLTFEGPCKSKQINIHLRGTLVAPSKDAWPSDKKDK--WIKFYDIDGLTISGG-- 129
           +YLL    F GPCKS + N  + GTL A +K    SD KDK  W+   D++ L+I GG  
Sbjct: 112 TYLLKSTRFRGPCKSLR-NFQILGTLSASTKR---SDYKDKNHWLILEDVNNLSIDGGST 171

Query: 130 GQIDGQGSSWWK-----DCEEHC-KRPTALFFHNCNGLRLSNMKHINSGKNHISINVCND 189
           G I+G G +WW+     D  + C K PTAL  +N   L + N++  N+ +  ISI  CN 
Sbjct: 172 GIINGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNK 231

Query: 190 VIVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGP 249
           V VSN+ ITAP +SPNTDGI I+ + N+ + NS + TGDDC++I +G+ N+ I  +TCGP
Sbjct: 232 VEVSNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGP 291

Query: 250 GHGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNT 309
           GHGISIGSLG+D     V  I V    F  + NGVRIKT++GG G A+NI F+ I + N 
Sbjct: 292 GHGISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENV 351

Query: 310 KNPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCS-RARCTNIVMN 369
           KNPIIIDQ Y       +++E  ++V +V Y+ + GTSA + AI LNCS +  C  IV+ 
Sbjct: 352 KNPIIIDQDYCDKDKC-EDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLE 411

Query: 370 NVNIKTSTPGKEAKAFCQNVEGK 382
           NV IK  T      A C+N   K
Sbjct: 412 NVKIKGGT------ASCKNANVK 421

BLAST of Cla010684 vs. Swiss-Prot
Match: QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2)

HSP 1 Score: 297.7 bits (761), Expect = 1.8e-79
Identity = 158/369 (42.82%), Postives = 226/369 (61.25%), Query Frame = 1

Query: 22  SASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFEGP 81
           S+  +FNV  +GA  NG  DDS+AF++AW+  C +T GI  +  P  + Y+L  +TF GP
Sbjct: 66  SSPRSFNVNTFGAKANGN-DDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGP 125

Query: 82  CKSKQINIHLRGTLVAPSKDAW--PSDKKDK--WIKFYDIDGLTISGGGQIDGQGSSWW- 141
           CKS  I   + G +     +AW  PSD K++  WI F +++ L + GGG+IDG G  WW 
Sbjct: 126 CKSSLIIFKIYGRI-----EAWENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP 185

Query: 142 KDCEEHCK-----RPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPD 201
           K C+ + +      PTA+ F  CN LR+SN++  N+ + H++   C +V   N+ +T+P 
Sbjct: 186 KSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPA 245

Query: 202 ESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGED 261
           +SPNTDGI +S + N+LI++S + TGDDC++I +GS N+   G+TCGPGHGISIGSLGED
Sbjct: 246 DSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGED 305

Query: 262 RKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTA 321
                V N+ V+     GT NGVRIKTW+GG+G A+NI F+ I + N  NPIII+Q Y  
Sbjct: 306 NSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCD 365

Query: 322 LASARKNKELNIKVSDVTYREVYGTSANENAIILNCSR-ARCTNIVMNNVNIKTSTPGKE 380
              A   ++  ++VS+V Y+ + GTS+   A+   CS+   C  I M NV +   T    
Sbjct: 366 RVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDV 425

BLAST of Cla010684 vs. Swiss-Prot
Match: PGLR_PERAE (Polygalacturonase OS=Persea americana PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 5.3e-79
Identity = 156/368 (42.39%), Postives = 218/368 (59.24%), Query Frame = 1

Query: 22  SASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFEGP 81
           S  ++ +V  +GA G+G TDD++AF +AWKD C +      L VP  K+YLL  +TF GP
Sbjct: 80  SPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSSGS---VLIVPENKNYLLKQITFSGP 139

Query: 82  CKSKQINIHLRGTLVAPSKDA-WPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKDCE 141
           CKS  + + +RGT+ A S  + W    + +WI+F DI  LT+ GGG  +G G +WW   +
Sbjct: 140 CKS-DLRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWW---D 199

Query: 142 EHCKR---------PTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPD 201
             CKR         PTAL F +C  L +S++   +S K H+S + C DVI SN+ +TAP+
Sbjct: 200 SSCKRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPE 259

Query: 202 ESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGED 261
            SPNTDGI I+ +  + + NS + TGDDC++I +GS  +    +TCGPGHGISIGSLG+ 
Sbjct: 260 HSPNTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDR 319

Query: 262 RKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTA 321
                V  + V       T NG+RIKTW+GG G A+NI F+ I + N  NPIIIDQYY  
Sbjct: 320 NSEAHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCD 379

Query: 322 LASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRAR-CTNIVMNNVNIKTSTPGKE 379
                  +E  +KVS+V Y  + GTSA+E A+  +CS++  C   ++ N+N+     GKE
Sbjct: 380 SKDPCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINL-VGNGGKE 438

BLAST of Cla010684 vs. Swiss-Prot
Match: ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 293.9 bits (751), Expect = 2.6e-78
Identity = 164/369 (44.44%), Postives = 222/369 (60.16%), Query Frame = 1

Query: 20  SVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFE 79
           S+   +  +V  +GA G+GKTDD+QAF+ AWK  C ++ G   L VP   +YLL  +   
Sbjct: 61  SLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKAC-SSNGAVNLLVPKGNTYLLKSIQLT 120

Query: 80  GPCKSKQINIHLRGTLVAPSKDAWPSDKKD--KWIKFYDIDGLTISGG--GQIDGQGSSW 139
           GPC S  + + + GTL A  K    SD KD  KWI F  ++ L++ GG  G +DG G +W
Sbjct: 121 GPCNSI-LTVQIFGTLSASQKR---SDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETW 180

Query: 140 WKD-CEEH-----CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITA 199
           W++ C+ +      K PTAL F+N   L + N+K  N+ +  ISI  C++V VSN+ +TA
Sbjct: 181 WQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTA 240

Query: 200 PDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLG 259
           P +SPNTDGI I+ + N+ +  S + TGDDC++I +GS N+ I  +TCGPGHGISIGSLG
Sbjct: 241 PADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLG 300

Query: 260 EDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYY 319
           +D     V  + V      GT NGVRIKT++GG G A NI F+ I + N KNPIIIDQ Y
Sbjct: 301 DDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDY 360

Query: 320 TALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSR-ARCTNIVMNNVNIKTSTPG 378
              +     K   ++V +V YR++ GTSA+ENAI  NCS+   C  IV++ VNI      
Sbjct: 361 CDKSKCTTEKSA-VQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------ 417

BLAST of Cla010684 vs. TrEMBL
Match: A0A0A0KZ95_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 1.2e-165
Identity = 275/396 (69.44%), Postives = 332/396 (83.84%), Query Frame = 1

Query: 1   MKLFFVFVLVLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGI 60
           MKL    + + S+ IL+SW+V  SS+FNVL YGA GNGKTDDS+AFL+AW DVC ATE  
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLHVPLAKSYLLNPLTFEGPCKSKQINIHLRGTLVAPSKDAWPSDKKDKWIKFYDIDGL 120
           PTLHVP  K+YLLNPL F+GPCKSKQ+     GTL+AP+KD W SD K +WI+F+DI+GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLG----GTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 TISGGGQIDGQGSSWWKDCE-EHCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCND 180
            + GGG+ DGQGS WWKDC+ +HCKRPTALFFHNCNGL++  MKHINS KNHISIN+C+D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 VIVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGP 240
           VI S++HI+AP++SPNTDGIDIS S N+ I++SFMATGDDC+AINNGSSNINI G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNT 300
           GHGISIGSLG+D +YN VEN+ VSNC  + TQNG+RIKTWEGGYGYA+NITFEKIT+ N 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRARCTNIVMNN 360
           KNPIIIDQYY++ A +RK K ++IKVSDVTYREV GTSANE+AI  NCS+ARC+NI+++N
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

Query: 361 VNIKTSTPGKEAKAFCQNVEGKAELAVPTVPCLSKS 396
           VNIK + PG++AKA CQN +GK   AVP V CLSKS
Sbjct: 361 VNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLSKS 392

BLAST of Cla010684 vs. TrEMBL
Match: A0A067JZL5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 8.2e-127
Identity = 230/389 (59.13%), Postives = 290/389 (74.55%), Query Frame = 1

Query: 10  VLSVVILNS-WSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLA 69
           VL+V IL+  W V+ASS+FNVL YGA G+GKTD+SQA LRAW+D+C AT G PTL VP  
Sbjct: 4   VLTVTILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAG 63

Query: 70  KSYLLNPLTFEGPCKSKQINIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQ 129
            ++LL P+ F+GPCKSK ++I ++GT+VA +  +AW  DK   W++F D++GL I GGGQ
Sbjct: 64  GTFLLKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKDK-GSWLQFSDVNGLIIDGGGQ 123

Query: 130 IDGQGSSWWKDCEEH--CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNI 189
           IDGQG  WWK C+E   C RPTAL FHNCNGL+LS +KH+NS +NHISIN C+DV + ++
Sbjct: 124 IDGQGGLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHL 183

Query: 190 HITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISI 249
           +ITAP +SPNTDGIDIS S ++ I+NS + TGDDC+AIN  SS INI G++CGPGHGIS+
Sbjct: 184 YITAPQDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISV 243

Query: 250 GSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIII 309
           GSLG++  Y TVE + V NC FKGTQNGVRIKTW+GG GYAR ITF+ I L  ++NPIII
Sbjct: 244 GSLGKNGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIII 303

Query: 310 DQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRAR-CTNIVMNNVNIKT 369
           DQ Y         +  N+ +SDVTYR + G+S+ E AI LNC R R CTNIVM+++ I  
Sbjct: 304 DQNYFC-RFPYSAESSNVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIVP 363

Query: 370 STPGKEAKAFCQNVEGKAELAVPTVPCLS 394
           S+PG    AFC N  G + LA PTVPCLS
Sbjct: 364 SSPGMHLHAFCNNAYGISTLASPTVPCLS 390

BLAST of Cla010684 vs. TrEMBL
Match: U5FZU8_POPTR (Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_0012s12010g PE=3 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 1.5e-120
Identity = 218/380 (57.37%), Postives = 272/380 (71.58%), Query Frame = 1

Query: 20  SVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFE 79
           S +    FNVL YGA GNGK D + AF +AW D C+A+E +PTL VP  K++LL  ++F 
Sbjct: 11  SEATGRRFNVLDYGADGNGKIDATPAFQKAWGDFCQASEEMPTLEVPAGKTFLLKSVSFS 70

Query: 80  GPCKSKQINIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKD 139
           GPC SK  ++ + GT+VAP+  D+W  D  +KWI F  + GL + GGG+ DG+G +WWK 
Sbjct: 71  GPCMSKNPHVLIEGTIVAPNSFDSWDDDDYEKWIGFTAVVGLIVDGGGRFDGRGEAWWKA 130

Query: 140 CEEH----CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPDESP 199
           C ++     KR  AL F+ CNGLRLSN+ H+NS K HI IN C+DV VSN+ I APDESP
Sbjct: 131 CNDNDSACSKRRQALHFNKCNGLRLSNLHHVNSQKGHICINACDDVEVSNLQILAPDESP 190

Query: 200 NTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGEDRKY 259
           NTDGIDIS SN+V I +SF+ TGDDC+AIN  S++IN+ GV CGPGHGISIGSLG+D  Y
Sbjct: 191 NTDGIDISESNHVNIHDSFIGTGDDCIAINGFSTSINVTGVKCGPGHGISIGSLGKDGAY 250

Query: 260 NTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTALAS 319
            TVE++ V +C FKGTQNGVRIKTWE G GY R ITFE IT  N++NPIIIDQ Y    +
Sbjct: 251 ETVEDVHVKSCAFKGTQNGVRIKTWETGSGYVRKITFEDITFVNSENPIIIDQQYNPNGN 310

Query: 320 ARKNKELNIKVSDVTYREVYGTSANENAIILNCS-RARCTNIVMNNVNIKTSTPGKEAKA 379
              +    IK+SDVTYR V G+SA+E AI LNC+ +A CTNIVM+NV I +S PGK+ +A
Sbjct: 311 RGGS---GIKISDVTYRNVRGSSADEVAIALNCAGKAACTNIVMDNVKITSSNPGKQIRA 370

Query: 380 FCQNVEGKAELAVPTVPCLS 394
            C N +G A  A PTVPCLS
Sbjct: 371 SCNNAKGTAISASPTVPCLS 387

BLAST of Cla010684 vs. TrEMBL
Match: M5VSG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023489mg PE=3 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 4.6e-114
Identity = 207/385 (53.77%), Postives = 273/385 (70.91%), Query Frame = 1

Query: 12  SVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVC-EATEGIPTLHVPLAKS 71
           S   L +W    ++ FNV+ YGA GNG  DD++AFL+AW  +C  +T+G  TL VP  K 
Sbjct: 54  SKAFLEAWEAKTNT-FNVVDYGAVGNGLVDDTKAFLKAWGAMCASSTQGTLTLVVPKGKR 113

Query: 72  YLLNPLTFEGPCKSKQINIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQID 131
           +LLN + FEGPC++ ++N  ++G++VAP+   AW +  K+ WI+F  + GL+++GGG+ID
Sbjct: 114 FLLNAVAFEGPCRASKVNFQIQGSIVAPNNIGAWTN--KEMWIQFSKVQGLSVNGGGRID 173

Query: 132 GQGSSWWKDCE-EHCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHIT 191
           G G+ WWK C  E C+RPTAL F+ C+G +LS +   NS KNHISI  CN   V  + I 
Sbjct: 174 GNGAVWWKACGGEGCQRPTALHFNKCHGFQLSRLAIFNSPKNHISICSCNGPRVFGLFIW 233

Query: 192 APDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSL 251
           AP +SPNTDGIDIS+S  V I++S +ATGDDC+AIN+GSS I I  + CGPGHGISIGSL
Sbjct: 234 APKDSPNTDGIDISQSTRVTIQSSHIATGDDCIAINSGSSYIKIRDIKCGPGHGISIGSL 293

Query: 252 GEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQY 311
           G+  +Y+ VE + VSNC FKGT NGVRIKTWEGG GYAR ITFE IT  +TKNPIIIDQ+
Sbjct: 294 GQHGEYSQVEEVQVSNCTFKGTTNGVRIKTWEGGSGYARKITFEGITFEDTKNPIIIDQH 353

Query: 312 YTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRARCTNIVMNNVNIKTSTPG 371
           Y A  ++  +   +++VSDVTYR + GT A+E AI L C+   CTNIVM+NV IK+  P 
Sbjct: 354 YFAKPTSAMDISKSVQVSDVTYRNINGTCADEKAISLACAGGGCTNIVMDNVTIKSDLPN 413

Query: 372 KEAKAFCQNVEGKAELAVPTVPCLS 394
           K + A+C N  G +  +VP+VPCLS
Sbjct: 414 KRSYAYCDNAHGTSSFSVPSVPCLS 435

BLAST of Cla010684 vs. TrEMBL
Match: A0A0S3RVQ3_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G213800 PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.3e-113
Identity = 219/405 (54.07%), Postives = 270/405 (66.67%), Query Frame = 1

Query: 1   MKLFFVF----VLVLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEA 60
           MKL FV     V   S  +     + A   FNV+ YGA GNG+TDDSQAF++AWKD C A
Sbjct: 1   MKLLFVALGMCVFAYSACVWGRMELHAGPRFNVIDYGAMGNGETDDSQAFMKAWKDACNA 60

Query: 61  TEGIPTLHVPLAKSYLLNPLTFEGPCKSKQINIHLRGTLVAPS--KDAWPSDK--KDKWI 120
           + G PTL +P  KS++L PL F GPCK   ++I L+GT++AP   KD W   K  +  WI
Sbjct: 61  SNGTPTLLIPKEKSFMLQPLFFSGPCKPSILHIKLKGTIIAPKNIKD-WKLGKGTRKAWI 120

Query: 121 KFYDIDGLTISGGGQIDGQGSSWWKDC-EEHCKRPTALFFHNCNGLRLSNMKHINSGKNH 180
           +F  I+GL I GGGQIDGQG+ WW         RPTAL F  CN L LS + HINS KNH
Sbjct: 121 RFKRINGLVIRGGGQIDGQGAPWWNAYFNSEINRPTALHFSECNNLFLSGLTHINSPKNH 180

Query: 181 ISINVCNDVIVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNIN 240
           ISIN CN   +S +H  APDESPNTDGIDIS S+N+LIKNS M TGDDC+AIN+GS+ I+
Sbjct: 181 ISINRCNGSFISKLHFIAPDESPNTDGIDISYSSNILIKNSKMETGDDCIAINHGSTFIS 240

Query: 241 IVGVTCGPGHGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITF 300
           ++GV CGPGHGISIGSLG++  ++TVE I+V NC F  T NG RIKTW GG GYAR ITF
Sbjct: 241 VIGVYCGPGHGISIGSLGKNGAHHTVEEIYVRNCTFNRTTNGARIKTWIGGKGYARKITF 300

Query: 301 EKITLTNTKNPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSR-A 360
           + I L   KNPIIIDQ Y    SA       ++VSDV+Y  V GTS++ +AI L+C +  
Sbjct: 301 KDIILVEAKNPIIIDQQYNPYDSAEA-----VRVSDVSYDNVRGTSSSPHAIKLHCDKNL 360

Query: 361 RCTNIVMNNVNIKTSTPGKEAKAFCQNVEGKAELAVPTVPCLSKS 396
            CTNIV+  +NI T+  GK+  A C+N++G      P VPCLS S
Sbjct: 361 GCTNIVLKGINI-TTIAGKKTYASCKNIKGVCSFCNPHVPCLSHS 398

BLAST of Cla010684 vs. NCBI nr
Match: gi|778697492|ref|XP_011654334.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus])

HSP 1 Score: 590.9 bits (1522), Expect = 1.7e-165
Identity = 275/396 (69.44%), Postives = 332/396 (83.84%), Query Frame = 1

Query: 1   MKLFFVFVLVLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGI 60
           MKL    + + S+ IL+SW+V  SS+FNVL YGA GNGKTDDS+AFL+AW DVC ATE  
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLHVPLAKSYLLNPLTFEGPCKSKQINIHLRGTLVAPSKDAWPSDKKDKWIKFYDIDGL 120
           PTLHVP  K+YLLNPL F+GPCKSKQ+     GTL+AP+KD W SD K +WI+F+DI+GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQLG----GTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 TISGGGQIDGQGSSWWKDCE-EHCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCND 180
            + GGG+ DGQGS WWKDC+ +HCKRPTALFFHNCNGL++  MKHINS KNHISIN+C+D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 VIVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGP 240
           VI S++HI+AP++SPNTDGIDIS S N+ I++SFMATGDDC+AINNGSSNINI G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNT 300
           GHGISIGSLG+D +YN VEN+ VSNC  + TQNG+RIKTWEGGYGYA+NITFEKIT+ N 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRARCTNIVMNN 360
           KNPIIIDQYY++ A +RK K ++IKVSDVTYREV GTSANE+AI  NCS+ARC+NI+++N
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

Query: 361 VNIKTSTPGKEAKAFCQNVEGKAELAVPTVPCLSKS 396
           VNIK + PG++AKA CQN +GK   AVP V CLSKS
Sbjct: 361 VNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLSKS 392

BLAST of Cla010684 vs. NCBI nr
Match: gi|659108957|ref|XP_008454473.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo])

HSP 1 Score: 567.8 bits (1462), Expect = 1.5e-158
Identity = 266/395 (67.34%), Postives = 323/395 (81.77%), Query Frame = 1

Query: 1   MKLFFVFVLVLSVVILNSWSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGI 60
           MKL    +L  S+ IL+S +V +SS+FNVL YGA GNGKTDDS+AFL+AW DVC AT+  
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLHVPLAKSYLLNPLTFEGPCKSKQINIHLRGTLVAPSKDAWPSDKKDKWIKFYDIDGL 120
           PTLHVP  K+YLL+PL F+GPCKS Q+   L GTL+AP+KD WP+D K KW++F D++GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TISGGGQIDGQGSSWWKDCEEHCKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDV 180
            + GGG+ DGQGS WWK          ALFFHNCNGL+++ MKH+NS KNHISIN+CNDV
Sbjct: 121 NVQGGGRFDGQGSCWWK----------ALFFHNCNGLQMNGMKHVNSAKNHISINLCNDV 180

Query: 181 IVSNIHITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPG 240
           I S++HI+AP++SPNTDGIDIS+S N+ I++SF+ TGDDCVAINNGSSNINI  +TCGPG
Sbjct: 181 IFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGPG 240

Query: 241 HGISIGSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTK 300
           HGISIGSLG+D +YN VEN+ VSNC  + TQNG+RIKTWEGGYGYA+NITFEKITL N+K
Sbjct: 241 HGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNSK 300

Query: 301 NPIIIDQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRARCTNIVMNNV 360
           NPIIIDQYYT+ A +RK K ++IKVSDVTYREV GTSANE AI  NCS+ARC+NI+++NV
Sbjct: 301 NPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDNV 360

Query: 361 NIKTSTPGKEAKAFCQNVEGKAELAVPTVPCLSKS 396
           NIK + PG+EAKA CQNV+GK   AVP V CLSKS
Sbjct: 361 NIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLSKS 385

BLAST of Cla010684 vs. NCBI nr
Match: gi|802681849|ref|XP_012082168.1| (PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas])

HSP 1 Score: 461.8 bits (1187), Expect = 1.2e-126
Identity = 230/389 (59.13%), Postives = 290/389 (74.55%), Query Frame = 1

Query: 10  VLSVVILNS-WSVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLA 69
           VL+V IL+  W V+ASS+FNVL YGA G+GKTD+SQA LRAW+D+C AT G PTL VP  
Sbjct: 4   VLTVTILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAG 63

Query: 70  KSYLLNPLTFEGPCKSKQINIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQ 129
            ++LL P+ F+GPCKSK ++I ++GT+VA +  +AW  DK   W++F D++GL I GGGQ
Sbjct: 64  GTFLLKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKDK-GSWLQFSDVNGLIIDGGGQ 123

Query: 130 IDGQGSSWWKDCEEH--CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNI 189
           IDGQG  WWK C+E   C RPTAL FHNCNGL+LS +KH+NS +NHISIN C+DV + ++
Sbjct: 124 IDGQGGLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHL 183

Query: 190 HITAPDESPNTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISI 249
           +ITAP +SPNTDGIDIS S ++ I+NS + TGDDC+AIN  SS INI G++CGPGHGIS+
Sbjct: 184 YITAPQDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISV 243

Query: 250 GSLGEDRKYNTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIII 309
           GSLG++  Y TVE + V NC FKGTQNGVRIKTW+GG GYAR ITF+ I L  ++NPIII
Sbjct: 244 GSLGKNGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIII 303

Query: 310 DQYYTALASARKNKELNIKVSDVTYREVYGTSANENAIILNCSRAR-CTNIVMNNVNIKT 369
           DQ Y         +  N+ +SDVTYR + G+S+ E AI LNC R R CTNIVM+++ I  
Sbjct: 304 DQNYFC-RFPYSAESSNVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIVP 363

Query: 370 STPGKEAKAFCQNVEGKAELAVPTVPCLS 394
           S+PG    AFC N  G + LA PTVPCLS
Sbjct: 364 SSPGMHLHAFCNNAYGISTLASPTVPCLS 390

BLAST of Cla010684 vs. NCBI nr
Match: gi|566198073|ref|XP_006377005.1| (glycoside hydrolase family 28 family protein [Populus trichocarpa])

HSP 1 Score: 441.0 bits (1133), Expect = 2.1e-120
Identity = 218/380 (57.37%), Postives = 272/380 (71.58%), Query Frame = 1

Query: 20  SVSASSNFNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFE 79
           S +    FNVL YGA GNGK D + AF +AW D C+A+E +PTL VP  K++LL  ++F 
Sbjct: 11  SEATGRRFNVLDYGADGNGKIDATPAFQKAWGDFCQASEEMPTLEVPAGKTFLLKSVSFS 70

Query: 80  GPCKSKQINIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKD 139
           GPC SK  ++ + GT+VAP+  D+W  D  +KWI F  + GL + GGG+ DG+G +WWK 
Sbjct: 71  GPCMSKNPHVLIEGTIVAPNSFDSWDDDDYEKWIGFTAVVGLIVDGGGRFDGRGEAWWKA 130

Query: 140 CEEH----CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPDESP 199
           C ++     KR  AL F+ CNGLRLSN+ H+NS K HI IN C+DV VSN+ I APDESP
Sbjct: 131 CNDNDSACSKRRQALHFNKCNGLRLSNLHHVNSQKGHICINACDDVEVSNLQILAPDESP 190

Query: 200 NTDGIDISRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGEDRKY 259
           NTDGIDIS SN+V I +SF+ TGDDC+AIN  S++IN+ GV CGPGHGISIGSLG+D  Y
Sbjct: 191 NTDGIDISESNHVNIHDSFIGTGDDCIAINGFSTSINVTGVKCGPGHGISIGSLGKDGAY 250

Query: 260 NTVENIFVSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTALAS 319
            TVE++ V +C FKGTQNGVRIKTWE G GY R ITFE IT  N++NPIIIDQ Y    +
Sbjct: 251 ETVEDVHVKSCAFKGTQNGVRIKTWETGSGYVRKITFEDITFVNSENPIIIDQQYNPNGN 310

Query: 320 ARKNKELNIKVSDVTYREVYGTSANENAIILNCS-RARCTNIVMNNVNIKTSTPGKEAKA 379
              +    IK+SDVTYR V G+SA+E AI LNC+ +A CTNIVM+NV I +S PGK+ +A
Sbjct: 311 RGGS---GIKISDVTYRNVRGSSADEVAIALNCAGKAACTNIVMDNVKITSSNPGKQIRA 370

Query: 380 FCQNVEGKAELAVPTVPCLS 394
            C N +G A  A PTVPCLS
Sbjct: 371 SCNNAKGTAISASPTVPCLS 387

BLAST of Cla010684 vs. NCBI nr
Match: gi|743914770|ref|XP_011001322.1| (PREDICTED: probable polygalacturonase At3g15720 [Populus euphratica])

HSP 1 Score: 436.0 bits (1120), Expect = 6.9e-119
Identity = 217/373 (58.18%), Postives = 265/373 (71.05%), Query Frame = 1

Query: 27  FNVLHYGATGNGKTDDSQAFLRAWKDVCEATEGIPTLHVPLAKSYLLNPLTFEGPCKSKQ 86
           FNVL YGA GNGK D + AF +AW   C A+E +PTL VP  K++LL  ++F GPC SK 
Sbjct: 18  FNVLDYGADGNGKIDATPAFQKAWGHFCRASEEMPTLEVPAGKTFLLKSVSFSGPCMSKN 77

Query: 87  INIHLRGTLVAPSK-DAWPSDKKDKWIKFYDIDGLTISGGGQIDGQGSSWWKDCEEH--- 146
            ++ + GT+VAP+  D+W  D  +KWI F    GL + GGG+ DG+G +WWK C ++   
Sbjct: 78  PHVLIEGTIVAPNSFDSWDDDDYEKWIGFTASIGLIVDGGGRFDGRGEAWWKACNDNDSA 137

Query: 147 -CKRPTALFFHNCNGLRLSNMKHINSGKNHISINVCNDVIVSNIHITAPDESPNTDGIDI 206
             KR  AL F+ CNGLRLSN+ H+NS K HISIN C+DV VSN+ I APDESPNTDGIDI
Sbjct: 138 CSKRRQALHFNKCNGLRLSNLHHVNSQKGHISINACDDVEVSNLQILAPDESPNTDGIDI 197

Query: 207 SRSNNVLIKNSFMATGDDCVAINNGSSNINIVGVTCGPGHGISIGSLGEDRKYNTVENIF 266
           S SN+V I +SF+ TGDDC+AIN  S++IN+  V CGPGHGISIGSLG+D  Y TVE + 
Sbjct: 198 SESNHVNIHDSFIGTGDDCIAINGFSTSINVTSVQCGPGHGISIGSLGKDGAYETVEEVH 257

Query: 267 VSNCFFKGTQNGVRIKTWEGGYGYARNITFEKITLTNTKNPIIIDQYYTALASARKNKEL 326
           V +C FKGTQNGVRIKTWE G GY R ITFE IT  N++NPIIIDQ Y    +   +   
Sbjct: 258 VKSCAFKGTQNGVRIKTWESGSGYVRKITFEDITFVNSENPIIIDQQYNPNGNRGSS--- 317

Query: 327 NIKVSDVTYREVYGTSANENAIILNCS-RARCTNIVMNNVNIKTSTPGKEAKAFCQNVEG 386
            IK+SDVTYR V G+SA+E AI LNC+ RA CTNIVM+NV I +S PGK+ +A C N  G
Sbjct: 318 GIKISDVTYRNVQGSSADEVAIALNCAGRAACTNIVMDNVKITSSNPGKQIRASCNNAMG 377

Query: 387 KAELAVPTVPCLS 394
            A  A PTVPCLS
Sbjct: 378 TAISASPTVPCLS 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR3_ARATH3.1e-10351.19Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 S... [more]
ADPG1_ARATH3.7e-8045.17Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1[more]
QRT2_ARATH1.8e-7942.82Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2[more]
PGLR_PERAE5.3e-7942.39Polygalacturonase OS=Persea americana PE=2 SV=1[more]
ADPG2_ARATH2.6e-7844.44Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZ95_CUCSA1.2e-16569.44Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1[more]
A0A067JZL5_JATCU8.2e-12759.13Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1[more]
U5FZU8_POPTR1.5e-12057.37Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_001... [more]
M5VSG3_PRUPE4.6e-11453.77Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa023489mg PE=3 SV=1[more]
A0A0S3RVQ3_PHAAN1.3e-11354.07Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.04G213800 PE=... [more]
Match NameE-valueIdentityDescription
gi|778697492|ref|XP_011654334.1|1.7e-16569.44PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus][more]
gi|659108957|ref|XP_008454473.1|1.5e-15867.34PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo][more]
gi|802681849|ref|XP_012082168.1|1.2e-12659.13PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas][more]
gi|566198073|ref|XP_006377005.1|2.1e-12057.37glycoside hydrolase family 28 family protein [Populus trichocarpa][more]
gi|743914770|ref|XP_011001322.1|6.9e-11958.18PREDICTED: probable polygalacturonase At3g15720 [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR006626PbH1
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004650 polygalacturonase activity
This gene is associated with the following unigenes:
Unigene NameAnalysis NameSequence type in Unigene
WMU51802watermelon EST collection version 2.0transcribed_cluster

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla010684Cla010684.1mRNA


The following transcribed_cluster feature(s) are associated with this gene:

Feature NameUnique NameType
WMU51802WMU51802transcribed_cluster


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 60..381
score: 5.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 234..247
scor
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 257..278
score: 760.0coord: 227..247
score: 7300.0coord: 204..225
score: 520.0coord: 177..203
score: 44.0coord: 286..307
score: 7
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 14..392
score: 1.37E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 18..393
score: 4.2E
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 1..396
score: 1.6E
NoneNo IPR availablePANTHERPTHR31375:SF42SUBFAMILY NOT NAMEDcoord: 1..396
score: 1.6E