Cla009060 (gene) Watermelon (97103) v1

NameCla009060
Typegene
OrganismCitrullus. lanatus (Watermelon (97103) v1)
DescriptionPolygalacturonase (AHRD V1 ***- A8MRN3_ARATH); contains Interpro domain(s) IPR012334 Pectin lyase fold
LocationChr1 : 21255917 .. 21257968 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTCTTTTGGCCTTAGTTTGTGTAGTTTCCATTGCTATTTTAAGCTCGTGGTCTGTATTGGCATCATCTGACTTCAATGTTCTCGATTATGGAGCAATTGGAAATGGCAAAGTGGATGATTCAAAAGTAAGTAATTTAAGCTACATAGTGATTTTTTTTACATAAAAAAAGTATTTTTGATTGTTTATTTGATCGGTAATCGTATGGTAACTTGTTTTCTTTGTATTTTTCTTTGTGACAAGGCATTTTTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGACAGTCCAACATTACATGCACCCATTGGAAAAACTTACCTATTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAAGAAGTCAATTTTAATGTAGTTTATCTATTCCTATATTCCAATGGGAAAAAAAAAAAGCTACATTTACGCTTCATTTAGAATACATTAATTATGTAAATAAAATTTGCATTAGAAGTAATTATTGTATTATGTTTTTGTAGCTTAGTGGAACTGTAATGGCTCCAAGCAAGGATGAATGGCCAAGTGATAGTGAAGATAAATGGGTCCACTTTTTTAACATAGAAGGTTTGAATCTCAAAGGAGGTGGACAATTTGATGGTGAAGGCTCTTTTTGGTGGAAACATTGTTATCATAAACAATGTAAACGACCAACGGTGCGTTTTAAATTCCATTTCAACTTTTATATTTTTCAGTAACTTGCATGATTGATTTCACATTTATAACTATGCTTTGATGGTTTAATTTATACTCTCAATAATAATTATAATGTATGATTTTGTAGGCATTGTTTTTCCACAATTGCAACCGACTTCAAGTCAAAGAAATGAAACACATCAATAGTGGCAGGAACCACATCTCCATAAATGATTCCAATGATGTCATTATCTCTAACATTCACATTAGTGCTCCCGATGAAAGCCCTAACACAGATGGAATTGATATTTCTAAATCAAACAATGTTCTTATTCGAGATTCTTTTATAGCAACAGGTAAACGCATTATTACCGTTATCATTATTATTATTTCTTTGTATTTCATATAGTTTGGTTCATAATTATACATTATTATTTAATTTTTAACTTCATTGTAAAAATGTAGGTGATGATTGTATTGCCATTAATAATGGATCTTCCAATATCAATATAATTGGTGTCACTTGTGGCCCTGGCCATGGCATAAGGTAAAAATGACATTTTTTCTTTTAACTCTTTACAAAAATCATATTACATTACATTACATCTAGTCTTTACGTGTAATTCTTTTCCATGTTATCATTTCATTAAAACTTTATTAAGTTTGAGAAAAATTAACCATTGTCATTTCTTCTTCCCTACATATGTTGGGGTGTTTATTTGAATAATGTAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAACATTGTTGAAAATGTTTATGTAAGCAAATGCCTCTTGCGAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGTGCTAATACTTTTTTTTTATTTCTTATATGCAATTAATGTCTAATGCACGTTATATAAATTGTTATAGATAATTGATTTTTCTTGAATGTAATTACAGGGTGGACATGGATATGCACGAAACATCACATTCGAGAAAATAACACTCAAGAATGCAAAAAATCCAATCATTATTGATCAATACTACACATCTTTTGCATATTCAAGGAAAAATAAGGTATACAACAAAAATGAAAACCATCACCTACATCAGTCTTATTACATTTTGTTGATATAGTTTTATAATGTTGATTTTAATGTTAAAATTTTCCTAACTTTTTAGGGAATGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTAATGGAACCTCGGCAAATGAAGATGCAATTACTTTGAACTGCTCTAGAGCTCATTGCACAAATATTATATTGGATAATGTGAGCATTAAAACAACAGATCCAGGAAAAGAAGCCAAAACTATTTGCCAAAATGTTGATGGAAAATCTTTATCTGTTGTTCCAATTGTTCCTTGCTTATCTTAG

mRNA sequence

ATGAAGCTTCTTTTGGCCTTAGTTTGTGTAGTTTCCATTGCTATTTTAAGCTCGTGGTCTGTATTGGCATCATCTGACTTCAATGTTCTCGATTATGGAGCAATTGGAAATGGCAAAGTGGATGATTCAAAAGCATTTTTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGACAGTCCAACATTACATGCACCCATTGGAAAAACTTACCTATTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAAGAAGTCAATTTTAATCTTAGTGGAACTGTAATGGCTCCAAGCAAGGATGAATGGCCAAGTGATAGTGAAGATAAATGGGTCCACTTTTTTAACATAGAAGGTTTGAATCTCAAAGGAGGTGGACAATTTGATGGTGAAGGCTCTTTTTGGTGGAAACATTGTTATCATAAACAATGTAAACGACCAACGGCATTGTTTTTCCACAATTGCAACCGACTTCAAGTCAAAGAAATGAAACACATCAATAGTGGCAGGAACCACATCTCCATAAATGATTCCAATGATGTCATTATCTCTAACATTCACATTAGTGCTCCCGATGAAAGCCCTAACACAGATGGAATTGATATTTCTAAATCAAACAATGTTCTTATTCGAGATTCTTTTATAGCAACAGGTGATGATTGTATTGCCATTAATAATGGATCTTCCAATATCAATATAATTGGTGTCACTTGTGGCCCTGGCCATGGCATAAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAACATTGTTGAAAATGTTTATGTAAGCAAATGCCTCTTGCGAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGGTGGACATGGATATGCACGAAACATCACATTCGAGAAAATAACACTCAAGAATGCAAAAAATCCAATCATTATTGATCAATACTACACATCTTTTGCATATTCAAGGAAAAATAAGGGAATGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTAATGGAACCTCGGCAAATGAAGATGCAATTACTTTGAACTGCTCTAGAGCTCATTGCACAAATATTATATTGGATAATGTGAGCATTAAAACAACAGATCCAGGAAAAGAAGCCAAAACTATTTGCCAAAATGTTGATGGAAAATCTTTATCTGTTGTTCCAATTGTTCCTTGCTTATCTTAG

Coding sequence (CDS)

ATGAAGCTTCTTTTGGCCTTAGTTTGTGTAGTTTCCATTGCTATTTTAAGCTCGTGGTCTGTATTGGCATCATCTGACTTCAATGTTCTCGATTATGGAGCAATTGGAAATGGCAAAGTGGATGATTCAAAAGCATTTTTGAAAGCTTGGAATGATGTGTGTGGAGCAACAGAAGACAGTCCAACATTACATGCACCCATTGGAAAAACTTACCTATTGAACCCTCTCAAATTTCAGGGTCCTTGCAAGTCCAAAGAAGTCAATTTTAATCTTAGTGGAACTGTAATGGCTCCAAGCAAGGATGAATGGCCAAGTGATAGTGAAGATAAATGGGTCCACTTTTTTAACATAGAAGGTTTGAATCTCAAAGGAGGTGGACAATTTGATGGTGAAGGCTCTTTTTGGTGGAAACATTGTTATCATAAACAATGTAAACGACCAACGGCATTGTTTTTCCACAATTGCAACCGACTTCAAGTCAAAGAAATGAAACACATCAATAGTGGCAGGAACCACATCTCCATAAATGATTCCAATGATGTCATTATCTCTAACATTCACATTAGTGCTCCCGATGAAAGCCCTAACACAGATGGAATTGATATTTCTAAATCAAACAATGTTCTTATTCGAGATTCTTTTATAGCAACAGGTGATGATTGTATTGCCATTAATAATGGATCTTCCAATATCAATATAATTGGTGTCACTTGTGGCCCTGGCCATGGCATAAGCATTGGGAGCTTAGGAAAAGATGGTGAATATAACATTGTTGAAAATGTTTATGTAAGCAAATGCCTCTTGCGAGGCACTCAAAATGGAGTTAGAATCAAGACTTGGCAGGGTGGACATGGATATGCACGAAACATCACATTCGAGAAAATAACACTCAAGAATGCAAAAAATCCAATCATTATTGATCAATACTACACATCTTTTGCATATTCAAGGAAAAATAAGGGAATGAACATAAAAGTGAGTGATGTGACATATCGTGAAGTTAATGGAACCTCGGCAAATGAAGATGCAATTACTTTGAACTGCTCTAGAGCTCATTGCACAAATATTATATTGGATAATGTGAGCATTAAAACAACAGATCCAGGAAAAGAAGCCAAAACTATTTGCCAAAATGTTGATGGAAAATCTTTATCTGTTGTTCCAATTGTTCCTTGCTTATCTTAG

Protein sequence

MKLLLALVCVVSIAILSSWSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAHCTNIILDNVSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS
BLAST of Cla009060 vs. Swiss-Prot
Match: PGLR3_ARATH (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.5e-99
Identity = 189/376 (50.27%), Postives = 247/376 (65.69%), Query Frame = 1

Query: 23  ASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPC 82
           +S+  +V  +GA+G+G  DDS+AFLKAW  VC  T D   +  P G T++L PLKFQG C
Sbjct: 20  SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGMTFMLQPLKFQGSC 79

Query: 83  KSKEVNFNLSGTVMAPSKDEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHK 142
           KS  V   + G ++APSK  W  D +D+W+ F +IEGL ++G G+ +G+GS WW+H    
Sbjct: 80  KSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEINGQGSSWWEH---- 139

Query: 143 QCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGIDI 202
           +  RPTAL F +CN L++  + H++S   HI I++ N V IS++ I+AP+ SPNTDGID+
Sbjct: 140 KGSRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDV 199

Query: 203 SKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENVY 262
             S+NV+I+D  IATGDDCIAIN+G+SNI+I G+ CGPGHGISIGSLGKDGE   VENV 
Sbjct: 200 GASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVENVC 259

Query: 263 VSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYT--SFAYSRKNK 322
           V  C  RGT NG RIKTWQGG GYAR ITF  ITL N +NPIIIDQ+Y       ++  K
Sbjct: 260 VQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRK 319

Query: 323 GMNIKVSDVTYREVNGTSANEDAITLNCS-RAHCTNIILDNVSIKT--TDPGKEAKTICQ 382
              ++VS V +    GTS +E  +   CS R  CT I L ++ I+T  +  G+ A+  C 
Sbjct: 320 SSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCL 379

Query: 383 NVDGKSLSVVPIVPCL 394
           NV G S   VP + CL
Sbjct: 380 NVRGASTIAVPGLECL 389

BLAST of Cla009060 vs. Swiss-Prot
Match: QRT2_ARATH (Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 5.0e-77
Identity = 156/365 (42.74%), Postives = 214/365 (58.63%), Query Frame = 1

Query: 27  FNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKE 86
           FNV  +GA  NG  DDSKAF+KAW   C +T     + AP  + Y+L  + F GPCKS  
Sbjct: 71  FNVNTFGAKANGN-DDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGPCKSSL 130

Query: 87  VNFNLSGTVMAPSKDEWPSDSEDK--WVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHKQC 146
           + F + G + A    E PSD +++  W+ F N+  L ++GGG+ DG G  WW     K C
Sbjct: 131 IIFKIYGRIEAW---ENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP----KSC 190

Query: 147 K---------RPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESP 206
           K          PTA+ F  CN L+V  ++  N+ + H++  D  +V   N+ +++P +SP
Sbjct: 191 KINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPADSP 250

Query: 207 NTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEY 266
           NTDGI +S + N+LI+DS + TGDDCI+I +GS N+   G+TCGPGHGISIGSLG+D   
Sbjct: 251 NTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDNSE 310

Query: 267 NIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAY 326
             V NV V+K  L GT NGVRIKTWQGGHG A+NI F+ I +KN  NPIII+Q Y     
Sbjct: 311 AYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDRVE 370

Query: 327 SRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSR-AHCTNIILDNVSIKTTDPGKEAKT 380
           +   +   ++VS+V Y+ + GTS+   A+   CS+   C  I + NV +        +K 
Sbjct: 371 ACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVSKA 426

BLAST of Cla009060 vs. Swiss-Prot
Match: ADPG2_ARATH (Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 4.6e-75
Identity = 159/355 (44.79%), Postives = 216/355 (60.85%), Query Frame = 1

Query: 20  SVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQ 79
           S+   +  +V D+GA G+GK DD++AF+ AW   C ++  +  L  P G TYLL  ++  
Sbjct: 61  SLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKAC-SSNGAVNLLVPKGNTYLLKSIQLT 120

Query: 80  GPCKSKEVNFNLSGTVMAPSKDEWPSDSED--KWVHFFNIEGLNLKGG--GQFDGEGSFW 139
           GPC S  +   + GT+ A  K    SD +D  KW+ F  +  L++ GG  G  DG G  W
Sbjct: 121 GPCNSI-LTVQIFGTLSASQKR---SDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETW 180

Query: 140 WKHCYH----KQC-KRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISA 199
           W++       K C K PTAL F+N   L VK +K  N+ +  ISI   ++V +SN+ ++A
Sbjct: 181 WQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTA 240

Query: 200 PDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLG 259
           P +SPNTDGI I+ + N+ + +S I TGDDCI+I +GS N+ I  +TCGPGHGISIGSLG
Sbjct: 241 PADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLG 300

Query: 260 KDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYY 319
            D     V  V V    L GT NGVRIKT+QGG G A NI F+ I + N KNPIIIDQ Y
Sbjct: 301 DDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDY 360

Query: 320 TSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAH-CTNIILDNVSIK 365
              +     K   ++V +V YR+++GTSA+E+AIT NCS+ + C  I+LD V+IK
Sbjct: 361 CDKSKCTTEKSA-VQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIK 409

BLAST of Cla009060 vs. Swiss-Prot
Match: PGLR5_ARATH (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.0e-74
Identity = 148/374 (39.57%), Postives = 216/374 (57.75%), Query Frame = 1

Query: 28  NVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKEV 87
           +V ++GA GNG  DD+KAF  AW   C +   +  L  P   T LL P+   GPCK++ +
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRIL-VPENYTCLLRPIDLSGPCKAR-L 113

Query: 88  NFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWW----KHCYHK 147
              +SGT++AP+  D W   +  KW++F  +  L ++GGG  +G G  WW    KH +  
Sbjct: 114 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 173

Query: 148 QCK-RPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGID 207
            C+  PTAL FH C  ++V+ +  I+S + HI++     V IS + + AP  SPNTDGI 
Sbjct: 174 PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIH 233

Query: 208 ISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENV 267
           IS S  ++I ++ ++TGDDCI+I   S+ I+I  + CGPGHGISIGSLGK   +  V ++
Sbjct: 234 ISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDI 293

Query: 268 YVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAYSRKNKG 327
            V   ++  T NGVRIKTWQGG G    I F  I + N  NPIIIDQYY        N+ 
Sbjct: 294 TVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQT 353

Query: 328 MNIKVSDVTYREVNGTSANEDAITLNCS-RAHCTNIILDNVSIKTTDPGKEAKTICQNVD 387
             I + ++++  V GTSA+++AI ++CS  + C NI+L ++ ++ ++     ++ C    
Sbjct: 354 SAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAY 413

Query: 388 GKSLSVVPIVPCLS 395
           G S   V   PCLS
Sbjct: 414 GSSSGQVYPPPCLS 425

BLAST of Cla009060 vs. Swiss-Prot
Match: ADPG1_ARATH (Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.3e-74
Identity = 165/383 (43.08%), Postives = 228/383 (59.53%), Query Frame = 1

Query: 15  ILSSWSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLN 74
           I S  + L +S  +V ++GA G+GK DD++AF KAW   C +T    T   P GKTYLL 
Sbjct: 56  ISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYLLK 115

Query: 75  PLKFQGPCKSKEVNFNLSGTVMAPSKDEWPSDSEDK--WVHFFNIEGLNLKGG--GQFDG 134
             +F+GPCKS   NF + GT+ A +K    SD +DK  W+   ++  L++ GG  G  +G
Sbjct: 116 STRFRGPCKSLR-NFQILGTLSASTKR---SDYKDKNHWLILEDVNNLSIDGGSTGIING 175

Query: 135 EGSFWWKHCYH----KQC-KRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISN 194
            G  WW++       K C K PTAL  +N   L VK ++  N+ +  ISI   N V +SN
Sbjct: 176 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 235

Query: 195 IHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGIS 254
           + I+AP +SPNTDGI I+ + N+ + +S I TGDDCI+I +G+ N+ I  +TCGPGHGIS
Sbjct: 236 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGIS 295

Query: 255 IGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPII 314
           IGSLG D     V  + V       + NGVRIKT+QGG G A+NI F+ I ++N KNPII
Sbjct: 296 IGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPII 355

Query: 315 IDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAH-CTNIILDNVSIK 374
           IDQ Y       +++   ++V +V Y+ ++GTSA + AITLNCS  + C  I+L+NV IK
Sbjct: 356 IDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVKIK 415

Query: 375 TTDPGKEAKTICQNVDGKSLSVV 388
               G  A   C+N + K+   V
Sbjct: 416 ----GGTAS--CKNANVKNQGTV 426

BLAST of Cla009060 vs. TrEMBL
Match: A0A0A0KZ95_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 2.2e-185
Identity = 309/394 (78.43%), Postives = 351/394 (89.09%), Query Frame = 1

Query: 1   MKLLLALVCVVSIAILSSWSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDS 60
           MKLL A + + SIAILSSW+VL SSDFNVLDYGAIGNGK DDSKAFLKAWNDVCGATE+S
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDEWPSDSEDKWVHFFNIEGL 120
           PTLH P GKTYLLNPLKFQGPCKSK+    L GT+MAP+KDEW SD + +W+ FF+IEGL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 NLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSND 180
           NL+GGG+FDG+GS WWK C HK CKRPTALFFHNCN LQ+K MKHINS +NHISIN  +D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 VIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGP 240
           VI S++HISAP++SPNTDGIDIS+S N+ I DSF+ATGDDCIAINNGSSNINI G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYN+VENV+VS CLLR TQNG+RIKTW+GG+GYA+NITFEKIT+KNA
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAHCTNIILDN 360
           KNPIIIDQYY+S+AYSRK KGM+IKVSDVTYREVNGTSANEDAIT NCS+A C+NIILDN
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

Query: 361 VSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
           V+IK T+PG++AK +CQN DGK LS VP V CLS
Sbjct: 361 VNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLS 390

BLAST of Cla009060 vs. TrEMBL
Match: A0A067JZL5_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 7.4e-120
Identity = 217/390 (55.64%), Postives = 277/390 (71.03%), Query Frame = 1

Query: 10  VVSIAILSS-WSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIG 69
           V+++ ILS  W V ASS FNVLDYGAIG+GK D+S+A L+AW D+CGAT  +PTL  P G
Sbjct: 4   VLTVTILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAG 63

Query: 70  KTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQ 129
            T+LL P++FQGPCKSK ++  + GT++A +  + W  D +  W+ F ++ GL + GGGQ
Sbjct: 64  GTFLLKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKD-KGSWLQFSDVNGLIIDGGGQ 123

Query: 130 FDGEGSFWWKHCYH-KQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNI 189
            DG+G  WWK C   K C RPTAL FHNCN LQ+  +KH+NS RNHISIN  +DV + ++
Sbjct: 124 IDGQGGLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHL 183

Query: 190 HISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISI 249
           +I+AP +SPNTDGIDIS S ++ IR+S I TGDDCIAIN  SS INI G++CGPGHGIS+
Sbjct: 184 YITAPQDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISV 243

Query: 250 GSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIII 309
           GSLGK+G Y  VE V+V  C  +GTQNGVRIKTWQGG GYAR ITF+ I L  ++NPIII
Sbjct: 244 GSLGKNGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIII 303

Query: 310 DQ-YYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAH-CTNIILDNVSIK 369
           DQ Y+  F YS ++   N+ +SDVTYR + G+S+ E AI LNC R   CTNI++D++ I 
Sbjct: 304 DQNYFCRFPYSAESS--NVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIV 363

Query: 370 TTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
            + PG      C N  G S    P VPCLS
Sbjct: 364 PSSPGMHLHAFCNNAYGISTLASPTVPCLS 390

BLAST of Cla009060 vs. TrEMBL
Match: U5FZU8_POPTR (Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_0012s12010g PE=3 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 7.6e-117
Identity = 209/373 (56.03%), Postives = 260/373 (69.71%), Query Frame = 1

Query: 27  FNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKE 86
           FNVLDYGA GNGK+D + AF KAW D C A+E+ PTL  P GKT+LL  + F GPC SK 
Sbjct: 18  FNVLDYGADGNGKIDATPAFQKAWGDFCQASEEMPTLEVPAGKTFLLKSVSFSGPCMSKN 77

Query: 87  VNFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHKQC- 146
            +  + GT++AP+  D W  D  +KW+ F  + GL + GGG+FDG G  WWK C      
Sbjct: 78  PHVLIEGTIVAPNSFDSWDDDDYEKWIGFTAVVGLIVDGGGRFDGRGEAWWKACNDNDSA 137

Query: 147 --KRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGIDI 206
             KR  AL F+ CN L++  + H+NS + HI IN  +DV +SN+ I APDESPNTDGIDI
Sbjct: 138 CSKRRQALHFNKCNGLRLSNLHHVNSQKGHICINACDDVEVSNLQILAPDESPNTDGIDI 197

Query: 207 SKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENVY 266
           S+SN+V I DSFI TGDDCIAIN  S++IN+ GV CGPGHGISIGSLGKDG Y  VE+V+
Sbjct: 198 SESNHVNIHDSFIGTGDDCIAINGFSTSINVTGVKCGPGHGISIGSLGKDGAYETVEDVH 257

Query: 267 VSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAYSRKNKGM 326
           V  C  +GTQNGVRIKTW+ G GY R ITFE IT  N++NPIIIDQ Y     +    G 
Sbjct: 258 VKSCAFKGTQNGVRIKTWETGSGYVRKITFEDITFVNSENPIIIDQQYNP---NGNRGGS 317

Query: 327 NIKVSDVTYREVNGTSANEDAITLNCS-RAHCTNIILDNVSIKTTDPGKEAKTICQNVDG 386
            IK+SDVTYR V G+SA+E AI LNC+ +A CTNI++DNV I +++PGK+ +  C N  G
Sbjct: 318 GIKISDVTYRNVRGSSADEVAIALNCAGKAACTNIVMDNVKITSSNPGKQIRASCNNAKG 377

Query: 387 KSLSVVPIVPCLS 395
            ++S  P VPCLS
Sbjct: 378 TAISASPTVPCLS 387

BLAST of Cla009060 vs. TrEMBL
Match: I1M7C8_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=2)

HSP 1 Score: 414.5 bits (1064), Expect = 1.5e-112
Identity = 207/401 (51.62%), Postives = 264/401 (65.84%), Query Frame = 1

Query: 1   MKLLLALVCVVSIAILSSWSVLASS-----DFNVLDYGAIGNGKVDDSKAFLKAWNDVCG 60
           M++L+A+  V+ +    S S +  +      FNV+DYGAIG+G  DDS+AFLKAW+ VC 
Sbjct: 1   MEVLIAIFFVLYLVSHCSCSRIREAAINLYTFNVMDYGAIGDGLTDDSQAFLKAWSMVCA 60

Query: 61  ATEDSPTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDE-WPSDSEDKWVHF 120
               + TL  P GKT++L PL+F GPC    V+F L G V+AP   E W      KW+ F
Sbjct: 61  MKNGAATLKVPPGKTFMLKPLQFSGPCSFSSVHFQLEGDVVAPKSTEAWKGQDSSKWIDF 120

Query: 121 FNIEGLNLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHIS 180
            N++GL + GGGQ DG GS WW  C  K C RPTAL   NCN LQ+   +H+NS RNHIS
Sbjct: 121 SNVDGLIIDGGGQIDGSGSVWWNSCKVKSCSRPTALSIQNCNNLQLTGTRHLNSARNHIS 180

Query: 181 INDSNDVIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINII 240
           IN+SN   I N+ I+AP +SPNTDGID+S+S+ +LI+ S IATGDDCIA+ +G+S +NI 
Sbjct: 181 INNSNHTHIFNVTITAPQDSPNTDGIDVSQSSYILIQRSTIATGDDCIAMKSGTSYVNIT 240

Query: 241 GVTCGPGHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEK 300
           G+TCGPGHGIS+GSLGK G    VE+V+V+ C  +G  NG+RIKTW GG GYARNI FE 
Sbjct: 241 GITCGPGHGISVGSLGKKGTCQTVEHVHVNNCNFKGADNGMRIKTWPGGCGYARNIKFEH 300

Query: 301 ITLKNAKNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNC-SRAHC 360
           I L N KNPIIIDQ Y +     K +   +++S VTYR VNGTS +E AI LNC + A C
Sbjct: 301 ILLTNTKNPIIIDQDYENVQNEDKKQTSEVQISGVTYRCVNGTSNSETAIILNCGAGAGC 360

Query: 361 TNIILDNVSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
           T+I +D V+I +T  G      C N  G + S  P V CLS
Sbjct: 361 TDIFMDVVNITSTSSGSNVHASCNNAHGVAASTSPPVSCLS 401

BLAST of Cla009060 vs. TrEMBL
Match: I1LNR1_SOYBN (Uncharacterized protein OS=Glycine max PE=3 SV=2)

HSP 1 Score: 412.9 bits (1060), Expect = 4.3e-112
Identity = 208/401 (51.87%), Postives = 264/401 (65.84%), Query Frame = 1

Query: 1   MKLLLALVCVVSIAILSSWSVLASS-----DFNVLDYGAIGNGKVDDSKAFLKAWNDVCG 60
           M++L+A+  ++ +    S S L  +      FNV+DYGAIG+G  DDS+AFLKAW+ VC 
Sbjct: 1   MEVLIAIFFLLYLVSHCSCSRLREAAINLNTFNVMDYGAIGDGLTDDSQAFLKAWSMVCA 60

Query: 61  ATEDSPTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDE-WPSDSEDKWVHF 120
               + TL  P GKT++L PL+F GPC    V+F L G V+AP   E W      KW+ F
Sbjct: 61  MKNGAATLKVPPGKTFMLKPLQFSGPCSFSSVHFQLEGDVVAPKSTEAWKGQDSSKWIDF 120

Query: 121 FNIEGLNLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHIS 180
            N++GL + GGGQ DG GS WW  C  K C RPTAL  HNCN LQ+   +H+NS RNHIS
Sbjct: 121 SNVDGLIIDGGGQIDGSGSVWWNSCKVKSCSRPTALSIHNCNNLQLTGTRHLNSARNHIS 180

Query: 181 INDSNDVIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINII 240
           IN+SN   I N+ I+AP +SPNTDGID+S+S+ +LI+ S IATGDDCIA+N+G+S +NI 
Sbjct: 181 INNSNHTHIFNVTITAPQDSPNTDGIDVSQSSYILIQRSTIATGDDCIAMNSGTSYVNIT 240

Query: 241 GVTCGPGHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEK 300
           G+TCGPGHGIS+GSLGK G    VE+V+VS C  +G  NG+RIKTW GG GYARNI FE 
Sbjct: 241 GITCGPGHGISVGSLGKKGTCQTVEHVHVSNCNFKGADNGMRIKTWPGGCGYARNIKFEH 300

Query: 301 ITLKNAKNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNC-SRAHC 360
           I L N KNPIIIDQ Y + +         +++S VTYR VNGT  +E AI LNC + A C
Sbjct: 301 IVLTNTKNPIIIDQDYENTS--------EVQISGVTYRYVNGTCNSETAIILNCGAGAGC 360

Query: 361 TNIILDNVSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
           T+I +D V+I +T  G      C N  G + S  P V CLS
Sbjct: 361 TDIFMDLVNITSTSSGSNVLASCNNAHGVAASTSPPVSCLS 393

BLAST of Cla009060 vs. NCBI nr
Match: gi|778697492|ref|XP_011654334.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 3.2e-185
Identity = 309/394 (78.43%), Postives = 351/394 (89.09%), Query Frame = 1

Query: 1   MKLLLALVCVVSIAILSSWSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDS 60
           MKLL A + + SIAILSSW+VL SSDFNVLDYGAIGNGK DDSKAFLKAWNDVCGATE+S
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDEWPSDSEDKWVHFFNIEGL 120
           PTLH P GKTYLLNPLKFQGPCKSK+    L GT+MAP+KDEW SD + +W+ FF+IEGL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 NLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSND 180
           NL+GGG+FDG+GS WWK C HK CKRPTALFFHNCN LQ+K MKHINS +NHISIN  +D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 181 VIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGP 240
           VI S++HISAP++SPNTDGIDIS+S N+ I DSF+ATGDDCIAINNGSSNINI G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 241 GHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYN+VENV+VS CLLR TQNG+RIKTW+GG+GYA+NITFEKIT+KNA
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 301 KNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAHCTNIILDN 360
           KNPIIIDQYY+S+AYSRK KGM+IKVSDVTYREVNGTSANEDAIT NCS+A C+NIILDN
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

Query: 361 VSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
           V+IK T+PG++AK +CQN DGK LS VP V CLS
Sbjct: 361 VNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLS 390

BLAST of Cla009060 vs. NCBI nr
Match: gi|659108957|ref|XP_008454473.1| (PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo])

HSP 1 Score: 629.8 bits (1623), Expect = 3.2e-177
Identity = 299/394 (75.89%), Postives = 344/394 (87.31%), Query Frame = 1

Query: 1   MKLLLALVCVVSIAILSSWSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDS 60
           MKLLLA +   SIAILSS +VL+SSDFNVLDYGAIGNGK DDSKAFLKAWNDVCGAT+DS
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLHAPIGKTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSKDEWPSDSEDKWVHFFNIEGL 120
           PTLH P GKTYLL+PLKFQGPCKS +V F+L GT+MAP+KDEWP+D + KWV F ++EGL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 NLKGGGQFDGEGSFWWKHCYHKQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSND 180
           N++GGG+FDG+GS WWK           ALFFHNCN LQ+  MKH+NS +NHISIN  ND
Sbjct: 121 NVQGGGRFDGQGSCWWK-----------ALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VIISNIHISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGP 240
           VI S++HISAP++SPNTDGIDIS+S N+ I+DSFI TGDDC+AINNGSSNINI  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNA 300
           GHGISIGSLGKDGEYN+VENV+VS CLLR TQNG+RIKTW+GG+GYA+NITFEKITLKN+
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQYYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAHCTNIILDN 360
           KNPIIIDQYYTS+AYSRK KGM+IKVSDVTYREVNGTSANE+AIT NCS+A C+NIILDN
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

Query: 361 VSIKTTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
           V+IK T+PG+EAK +CQNVDGK LS VP V CLS
Sbjct: 361 VNIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLS 383

BLAST of Cla009060 vs. NCBI nr
Match: gi|802681849|ref|XP_012082168.1| (PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas])

HSP 1 Score: 438.7 bits (1127), Expect = 1.1e-119
Identity = 217/390 (55.64%), Postives = 277/390 (71.03%), Query Frame = 1

Query: 10  VVSIAILSS-WSVLASSDFNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIG 69
           V+++ ILS  W V ASS FNVLDYGAIG+GK D+S+A L+AW D+CGAT  +PTL  P G
Sbjct: 4   VLTVTILSFFWIVTASSSFNVLDYGAIGDGKTDNSQAVLRAWEDLCGATRGTPTLIVPAG 63

Query: 70  KTYLLNPLKFQGPCKSKEVNFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQ 129
            T+LL P++FQGPCKSK ++  + GT++A +  + W  D +  W+ F ++ GL + GGGQ
Sbjct: 64  GTFLLKPVRFQGPCKSKNLHIQIQGTIVASNNINAWGKD-KGSWLQFSDVNGLIIDGGGQ 123

Query: 130 FDGEGSFWWKHCYH-KQCKRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNI 189
            DG+G  WWK C   K C RPTAL FHNCN LQ+  +KH+NS RNHISIN  +DV + ++
Sbjct: 124 IDGQGGLWWKFCKEDKGCSRPTALSFHNCNGLQLSGLKHLNSQRNHISINGCSDVNLFHL 183

Query: 190 HISAPDESPNTDGIDISKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISI 249
           +I+AP +SPNTDGIDIS S ++ IR+S I TGDDCIAIN  SS INI G++CGPGHGIS+
Sbjct: 184 YITAPQDSPNTDGIDISASTHINIRNSVIRTGDDCIAINGFSSYINISGISCGPGHGISV 243

Query: 250 GSLGKDGEYNIVENVYVSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIII 309
           GSLGK+G Y  VE V+V  C  +GTQNGVRIKTWQGG GYAR ITF+ I L  ++NPIII
Sbjct: 244 GSLGKNGAYETVEEVHVQNCTFKGTQNGVRIKTWQGGAGYARKITFDNIILIASQNPIII 303

Query: 310 DQ-YYTSFAYSRKNKGMNIKVSDVTYREVNGTSANEDAITLNCSRAH-CTNIILDNVSIK 369
           DQ Y+  F YS ++   N+ +SDVTYR + G+S+ E AI LNC R   CTNI++D++ I 
Sbjct: 304 DQNYFCRFPYSAESS--NVYISDVTYRNIRGSSSVERAIDLNCGRKRGCTNIVMDDIEIV 363

Query: 370 TTDPGKEAKTICQNVDGKSLSVVPIVPCLS 395
            + PG      C N  G S    P VPCLS
Sbjct: 364 PSSPGMHLHAFCNNAYGISTLASPTVPCLS 390

BLAST of Cla009060 vs. NCBI nr
Match: gi|566198073|ref|XP_006377005.1| (glycoside hydrolase family 28 family protein [Populus trichocarpa])

HSP 1 Score: 428.7 bits (1101), Expect = 1.1e-116
Identity = 209/373 (56.03%), Postives = 260/373 (69.71%), Query Frame = 1

Query: 27  FNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKE 86
           FNVLDYGA GNGK+D + AF KAW D C A+E+ PTL  P GKT+LL  + F GPC SK 
Sbjct: 18  FNVLDYGADGNGKIDATPAFQKAWGDFCQASEEMPTLEVPAGKTFLLKSVSFSGPCMSKN 77

Query: 87  VNFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHKQC- 146
            +  + GT++AP+  D W  D  +KW+ F  + GL + GGG+FDG G  WWK C      
Sbjct: 78  PHVLIEGTIVAPNSFDSWDDDDYEKWIGFTAVVGLIVDGGGRFDGRGEAWWKACNDNDSA 137

Query: 147 --KRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGIDI 206
             KR  AL F+ CN L++  + H+NS + HI IN  +DV +SN+ I APDESPNTDGIDI
Sbjct: 138 CSKRRQALHFNKCNGLRLSNLHHVNSQKGHICINACDDVEVSNLQILAPDESPNTDGIDI 197

Query: 207 SKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENVY 266
           S+SN+V I DSFI TGDDCIAIN  S++IN+ GV CGPGHGISIGSLGKDG Y  VE+V+
Sbjct: 198 SESNHVNIHDSFIGTGDDCIAINGFSTSINVTGVKCGPGHGISIGSLGKDGAYETVEDVH 257

Query: 267 VSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAYSRKNKGM 326
           V  C  +GTQNGVRIKTW+ G GY R ITFE IT  N++NPIIIDQ Y     +    G 
Sbjct: 258 VKSCAFKGTQNGVRIKTWETGSGYVRKITFEDITFVNSENPIIIDQQYNP---NGNRGGS 317

Query: 327 NIKVSDVTYREVNGTSANEDAITLNCS-RAHCTNIILDNVSIKTTDPGKEAKTICQNVDG 386
            IK+SDVTYR V G+SA+E AI LNC+ +A CTNI++DNV I +++PGK+ +  C N  G
Sbjct: 318 GIKISDVTYRNVRGSSADEVAIALNCAGKAACTNIVMDNVKITSSNPGKQIRASCNNAKG 377

Query: 387 KSLSVVPIVPCLS 395
            ++S  P VPCLS
Sbjct: 378 TAISASPTVPCLS 387

BLAST of Cla009060 vs. NCBI nr
Match: gi|743914770|ref|XP_011001322.1| (PREDICTED: probable polygalacturonase At3g15720 [Populus euphratica])

HSP 1 Score: 422.9 bits (1086), Expect = 6.0e-115
Identity = 210/374 (56.15%), Postives = 259/374 (69.25%), Query Frame = 1

Query: 27  FNVLDYGAIGNGKVDDSKAFLKAWNDVCGATEDSPTLHAPIGKTYLLNPLKFQGPCKSKE 86
           FNVLDYGA GNGK+D + AF KAW   C A+E+ PTL  P GKT+LL  + F GPC SK 
Sbjct: 18  FNVLDYGADGNGKIDATPAFQKAWGHFCRASEEMPTLEVPAGKTFLLKSVSFSGPCMSKN 77

Query: 87  VNFNLSGTVMAPSK-DEWPSDSEDKWVHFFNIEGLNLKGGGQFDGEGSFWWKHCYHKQC- 146
            +  + GT++AP+  D W  D  +KW+ F    GL + GGG+FDG G  WWK C      
Sbjct: 78  PHVLIEGTIVAPNSFDSWDDDDYEKWIGFTASIGLIVDGGGRFDGRGEAWWKACNDNDSA 137

Query: 147 --KRPTALFFHNCNRLQVKEMKHINSGRNHISINDSNDVIISNIHISAPDESPNTDGIDI 206
             KR  AL F+ CN L++  + H+NS + HISIN  +DV +SN+ I APDESPNTDGIDI
Sbjct: 138 CSKRRQALHFNKCNGLRLSNLHHVNSQKGHISINACDDVEVSNLQILAPDESPNTDGIDI 197

Query: 207 SKSNNVLIRDSFIATGDDCIAINNGSSNINIIGVTCGPGHGISIGSLGKDGEYNIVENVY 266
           S+SN+V I DSFI TGDDCIAIN  S++IN+  V CGPGHGISIGSLGKDG Y  VE V+
Sbjct: 198 SESNHVNIHDSFIGTGDDCIAINGFSTSINVTSVQCGPGHGISIGSLGKDGAYETVEEVH 257

Query: 267 VSKCLLRGTQNGVRIKTWQGGHGYARNITFEKITLKNAKNPIIIDQYYTSFAYSRKNKGM 326
           V  C  +GTQNGVRIKTW+ G GY R ITFE IT  N++NPIIIDQ Y        N+G 
Sbjct: 258 VKSCAFKGTQNGVRIKTWESGSGYVRKITFEDITFVNSENPIIIDQQYN----PNGNRGS 317

Query: 327 N-IKVSDVTYREVNGTSANEDAITLNCS-RAHCTNIILDNVSIKTTDPGKEAKTICQNVD 386
           + IK+SDVTYR V G+SA+E AI LNC+ RA CTNI++DNV I +++PGK+ +  C N  
Sbjct: 318 SGIKISDVTYRNVQGSSADEVAIALNCAGRAACTNIVMDNVKITSSNPGKQIRASCNNAM 377

Query: 387 GKSLSVVPIVPCLS 395
           G ++S  P VPCLS
Sbjct: 378 GTAISASPTVPCLS 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PGLR3_ARATH3.5e-9950.27Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana GN=At3g15720 PE=1 S... [more]
QRT2_ARATH5.0e-7742.74Polygalacturonase QRT2 OS=Arabidopsis thaliana GN=QRT2 PE=1 SV=2[more]
ADPG2_ARATH4.6e-7544.79Polygalacturonase ADPG2 OS=Arabidopsis thaliana GN=ADPG2 PE=2 SV=2[more]
PGLR5_ARATH1.0e-7439.57Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana GN=At1g80170 PE=1 S... [more]
ADPG1_ARATH1.3e-7443.08Polygalacturonase ADPG1 OS=Arabidopsis thaliana GN=ADPG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KZ95_CUCSA2.2e-18578.43Uncharacterized protein OS=Cucumis sativus GN=Csa_4G025750 PE=3 SV=1[more]
A0A067JZL5_JATCU7.4e-12055.64Uncharacterized protein OS=Jatropha curcas GN=JCGZ_19407 PE=3 SV=1[more]
U5FZU8_POPTR7.6e-11756.03Glycoside hydrolase family 28 family protein OS=Populus trichocarpa GN=POPTR_001... [more]
I1M7C8_SOYBN1.5e-11251.62Uncharacterized protein OS=Glycine max PE=3 SV=2[more]
I1LNR1_SOYBN4.3e-11251.87Uncharacterized protein OS=Glycine max PE=3 SV=2[more]
Match NameE-valueIdentityDescription
gi|778697492|ref|XP_011654334.1|3.2e-18578.43PREDICTED: probable polygalacturonase At3g15720 [Cucumis sativus][more]
gi|659108957|ref|XP_008454473.1|3.2e-17775.89PREDICTED: probable polygalacturonase At3g15720 [Cucumis melo][more]
gi|802681849|ref|XP_012082168.1|1.1e-11955.64PREDICTED: probable polygalacturonase At3g15720 [Jatropha curcas][more]
gi|566198073|ref|XP_006377005.1|1.1e-11656.03glycoside hydrolase family 28 family protein [Populus trichocarpa][more]
gi|743914770|ref|XP_011001322.1|6.0e-11556.15PREDICTED: probable polygalacturonase At3g15720 [Populus euphratica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000743Glyco_hydro_28
IPR006626PbH1
IPR011050Pectin_lyase_fold/virulence
IPR012334Pectin_lyas_fold
Vocabulary: Molecular Function
TermDefinition
GO:0004650polygalacturonase activity
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071555 cell wall organization
biological_process GO:0005982 starch metabolic process
biological_process GO:0005985 sucrose metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0005575 cellular_component
molecular_function GO:0004650 polygalacturonase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cla009060Cla009060.1mRNA


Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 62..380
score: 4.7
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 235..248
scor
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 228..248
score: 6700.0coord: 178..204
score: 38.0coord: 287..308
score: 1200.0coord: 205..226
score: 280.0coord: 321..348
score: 3100.0coord: 258..279
score:
IPR011050Pectin lyase fold/virulence factorunknownSSF51126Pectin lyase-likecoord: 12..393
score: 5.01E
IPR012334Pectin lyase foldGENE3DG3DSA:2.160.20.10coord: 11..393
score: 1.8E
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 10..393
score: 3.0E
NoneNo IPR availablePANTHERPTHR31375:SF42SUBFAMILY NOT NAMEDcoord: 10..393
score: 3.0E

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cla009060Cla97C01G010540Watermelon (97103) v2wmwmbB239
Cla009060ClCG01G010770Watermelon (Charleston Gray)wcgwmB146
Cla009060Lsi02G011360Bottle gourd (USVL1VR-Ls)lsiwmB201
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cla009060Cucurbita pepo (Zucchini)cpewmB521
Cla009060Cucurbita maxima (Rimu)cmawmB197
Cla009060Cucurbita moschata (Rifu)cmowmB184