Cla006632 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla006632
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Diacylglycerol kinase-like protein (AHRD V1 **** O82359_ARATH); contains Interpro domain(s) IPR001206 Diacylglycerol kinase, catalytic region
Location	Chr6 : 2451305 .. 2455844 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAACACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCCGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGGTCGTCTTCTTTCTCTTCCTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGTGGTTCGTTTCTCGTCTCTTAATTTTCAGTTTCGTTGATAGGGAGAGGAATTCAGTTGAGAAATAGAAAATTTAGGATGTGGGTTTGCTGTATTTGTGCGGTAATTGAAGATTGTAATGCCTTGAGTTTATTGGAACTTCACTGGAGATTGTAATTTTTTATGTGCTTTTCAAGTTCTTGGGGCTATTGGTCATTGGGTGATTACTAGTTATGGATTTGTTCATATTTTAGCTCCTATGCAGGTGCAAATGGTAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGGTGAACATTATAATGTGAGATTTTTTTTTCTCTTGGTCCCTGTAGGGAATGCTTATGGGTCCAATGGTGTTCCTGGGTTTCGGCTTCACTATCTCCATTGTAGTTAATCTAGCTTATTGAGTTATGTTTAAGGAGCTTGTTGGAGTAAATGACTTGCAAAAGAAATACTAGATTTAAAGACTTTGGAGGGAGCTATTTTCTGACATAGTTTTTTCGTTTTATGACAACAAAATTTTAGTTAAACATGTTTGGAATTTGGATATCTGTTTTTTAGGAGTTGTTTTCCATATATTTCTTTTTCTATTTGTTGGCCTGTTTCTTAGAATAATTAATTTGTTTGTTAAACCTCCAATTGTGAATGTTTATGCTAGAAGGGTCGAAAAAGGCAATTTTGTCACAAGCAGCAAAGATAAGTAGTAGTTTTTATTATTAGGATTGTTAGTAGTTATTATATTTGAATCTGGTGTGTTAGGAAGTTATAACTTCCTTTGTTGGTTATGATTGTGACCCTCTCGAATTGGTCATATTTGATTGGTTGTTTGTTCTCACTTGGGAATTTTTAAGTAAATAAAGGATATCTATCATAGTTCTCCTTCAAAAGTCAGGACAACTAGTCTTGGCATTGTTAAAAGGAGAGTATTGCCAAGTACTTATAAGGATTACAAATATGTGCTTGCTTGGACTTTGACTCAGAAATCTAGTTTTAGTATCGATTAAGTATTCAGTTCTTTACATACAACCCACCATTTTAATTGAATAACCTTGTGATTCTAATTAAACCAGCCAGTTTACTATTGCTTTGATCTACATGGATAGCATCTTCAATGACAGAGGAAAACCTTTTTGACAGAACTATATACTTTTAGGTTCTTTCTCGGTATAAATTTTTTCTTATTTTCATGGCTACAGTCTATACACTTTTGGTCAATTGGTCATTTTTGTAGCCTCCTAAGGCGTGGGTTTGCTGCTTCCCTGTATACTTTATCTCATCAATGAAATGCTAGAATATTTCTCATTTTTAAAAGATAATAAAGATAATAAAATGCAAGTCCCTTATATTATCAACGGCATATTTGCATCTCATTTGTACATTTTCCACTGATCTGACTTTTTGTTTCTTTAAAGGAGCTATTATTTGCTTTTGTTGAGATTTCAAACTCGTTAATGATCCATATCTGCATGGATTATTTTTGTTATTTTGACTTATTTTTTCAAATTTCATCGAGGAGCCTAGAAAACCATAATAACTCTTTATCATGTTATAATGGAGTATATAAGTGAATGGTTCCATGTGAAATTTATCTTTCAACTAAACAATTCACTTTGGTGGACAGATATGTGAATCTTTGACGTCAGGTCCTTGCCATGCTATTGATATAACGAGGGAGGTCTGTCTGGATTCTTTCTTTGTGGGACTTCAAATAGTTACTAGCAACCTTTTCGTTGACTTACTGACTTATAGGCTATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTAACATTCGTTTATATTAGTTCAGTTTTCAATGCTTGACTCAGGCTTGATTTTCTAAATTTCCTTTTCTCTCTGTCGGTTTTGGTTTCGGTTAAACTTGAAAACCAAGCCAAAGTGCTTGCACACGTAAGTTTGATGATGTGATCGTCTGATTGGTGTATGACCAGTGTTTACTTTTATTTCTCTTGGACTCCTCTAAACTTTTTGAAGATATTATTTATAACTATCCATAGTTTTTGAACCCTGAGATTTCAGTGGTAACATCTAACCAATAGTGTTGCTTGAGTATGTCTTCTTCTCCTACTACTTAGCATTTAATAACGATCAGTTATCAAGGTCTGTTTTCTATAAAAATAACCTTTTAACTTGGCTTTGATTCTAGCAACCCCCCCTCTTGTATTGTCCATTAGATTTCCAGTAGATATAAGCAAGCATTTACAGGCTTTTTGTGAAAGTTTCTAGGAGTGAAGATCGGTTGCATACGTTTGTTTTGATGGATGAGGGAATTAACCTGCCTTCTGATTGACAGGTGGTTAATGGCTTCTTTTGGGGTGGAAAACCTGTTGTTAATAATGATGGAGAAGCTCGCAAATCAACAGCACTTGGTGTAAGAGATTCCTGCGTAGTTACAATCTTAAGCTGTCATTTCCATTATCTACTATAACTGCTAGTTTATCAGTCCGTGACTCCTTCAGATTATTTTCTTGAACTTATACTATTGAATTGTCGGACTACATACATTATTGTGCCTACTTGCAGATTATTCCCTTAGGGACAGGATCAGATTTTGCTAGAACATTTGGTTGGTAAGTGCATGTTATTGCATTGTGTTTTTGTACGATGTGAGACTTTAAATTAGCACTTCCTCCATGGGGTTAAGCAAACTCGGTCAACATTCTTTTAATTAGGAATAACAATCCTTTTGAAGCTGTGGACCGTATAGCGAAAGGTATCCCTCTTTCACTTTTTTCATCTTCCTCTCTTAGGTTTCTTTCAATGAAGTACTTTAAGTGGCTCTGTTTGTTCACATAAACATCACATTAGCATTGGTAGTTCATGTAAGATCTTCTGCAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGAGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGTAAGGTTAATCTAAACAGTATCATTGATGCTAACCGACAAACTATGGTAATTCAGGACATCTAATTATTATATTGCATGGATCTTTTTGCAGGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAAAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTTAAGATTAAGGTGAGCATATAAAAGAAGCTCTAGGTTTTTTTAGTTTGTTTTATGCAATAATTGTAGTGCGGTGCCTTAAGGTGGTTTCAAAATGTAGACCATTTTTGGGGGTGGGAGAGAGTATTTTCAACATCTTGATTTAATTTCATCTATGTAGAATGCTATGACTTACAGGTCGATGATGGTGAATGGGAGCTGTATCCACAAGTAACTGCACTATGCATTGGAAATGCTAAATATTTTGGTGGTGGTATGAAGATTGTGCCGAATGCCAATCCTTCCAATGGGAGTCTAGAGGTAGAATTCATTTCCGTGCAATTTTATCTGATTTCACTATTTCCTCCATTGTCTCCTTAACTTGTGTGATACTTGCCCTCTGCTTTCTTTGCAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAACGTAACATCAAGAAGGTTTGTCATCTCTTTAAGAATTACCATGTGTCTACTTTCCATTTTGTGTTTCAATTTTCACTCTCCAACATCTGTTTTATGCTGGAGACTTGTGTAAGTAAAGATGAAGAAACTATAACTGAACGGGAATTGCATGGAGAATTTGTAGTTTTCATTTCTGTGGAAGTTTTTGCTTGAGAAAAACTTACATTAAAACTTATTCGAGATGCTTTTTTCACCTAGCAGACAAGCCATAAGAAAAGTAATTGGTAAACTTCCTGGCATTCTGATTTATCCTCCACCAAAATTTGAAAGCTTTTGTGGTTATCATCATTTCCTTGTTAAATCTTTGATCACTTTGACGAACTCAGTTCGAAAAATTCGATTGGTATCAATGTACTCATCCATGGTTGAATACACAATTAAGCATGATTTCAGATTTCAGCTCCACTTCATTGAAATTCATTAGTTGGCAATGGTGTTCTTTTCTGAATAAAAACTTCCTACTGCTGCTTCTGGATGTGTTGGTCTACAACTATCATCAGTTTATTGTTTTGTTGTTCAAGCAGCGTCCGTTCGATCGAAGTGGAAGAAGTTTCAAGCAGCGGCAGCATTTATGTTCAATCTGATGGAGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

mRNA sequence

ATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAACACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCCGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGGTCGTCTTCTTTCTCTTCCTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGTGGTGCAAATGGTAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGGTGAACATTATAATATATGTGAATCTTTGACGTCAGGTCCTTGCCATGCTATTGATATAACGAGGGAGGCTATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGGTGGAAAACCTGTTGTTAATAATGATGGAGAAGCTCGCAAATCAACAGCACTTGGTATTATTCCCTTAGGGACAGGATCAGATTTTGCTAGAACATTTGGTTGGAATAACAATCCTTTTGAAGCTGTGGACCGTATAGCGAAAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGAGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAAAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTTAAGATTAAGGTCGATGATGGTGAATGGGAGCTGTATCCACAAGTAACTGCACTATGCATTGGAAATGCTAAATATTTTGGTGGTGGTATGAAGATTGTGCCGAATGCCAATCCTTCCAATGGGAGTCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAACGTAACATCAAGAAGCGTCCGTTCGATCGAAGTGGAAGAAGTTTCAAGCAGCGGCAGCATTTATGTTCAATCTGATGGAGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

Coding sequence (CDS)

ATGGTTGTTTCCTTTTGCTATCACTTAGCATTGGGACCTTCCATGGCGACAACACCCTTTATCACCAGGGCCGAGCAGCCCATCGCGCCCGATCTTTATGCCGATCGGTCAATTTTTCATCGTGGGTCGTCTTCTTTCTCTTCCTCTTCTGGCCGTCGCGATCTTGTTTTCGTTGTTAATCCCCGTGGTGCAAATGGTAAGACGGTGAAGGAATGGAAGAAGCTCGTTCCATATCTACGCACTCGCCTTGGTGAACATTATAATATATGTGAATCTTTGACGTCAGGTCCTTGCCATGCTATTGATATAACGAGGGAGGCTATTCGGGAAGGTGCTGATGCTGTGATTGCTGTAGGAGGAGATGGAACTTTTCATGAGGTGGTTAATGGCTTCTTTTGGGGTGGAAAACCTGTTGTTAATAATGATGGAGAAGCTCGCAAATCAACAGCACTTGGTATTATTCCCTTAGGGACAGGATCAGATTTTGCTAGAACATTTGGTTGGAATAACAATCCTTTTGAAGCTGTGGACCGTATAGCGAAAGGGTTGAGGTCCCGAATTGATGTTGGAGTTGTCAATGAAGAAAATGGAGAGTATCATTACTTTGCAAATGTTGCAGACATTCATTTGAGTGCAAAGGCTGGTTTTTATGCTGCAAGATACAAAAAATTTGGAAACTTATGCTATGTGATTGGTGCTTTGCAAGGCTTTATGGGACACCAAAATAAAGATTTTAAGATTAAGGTCGATGATGGTGAATGGGAGCTGTATCCACAAGTAACTGCACTATGCATTGGAAATGCTAAATATTTTGGTGGTGGTATGAAGATTGTGCCGAATGCCAATCCTTCCAATGGGAGTCTAGAGGTAGTAATTCTTCAGGATTTCAAGTGGTACGACTTTATTCTGAATTTACATAAAATCTACAATGGGACACATTTGACAGTGAAAAACGTAACATCAAGAAGCGTCCGTTCGATCGAAGTGGAAGAAGTTTCAAGCAGCGGCAGCATTTATGTTCAATCTGATGGAGAACACTTGGGGTTTCTTCCAAGGAAATTCCATATTCTACCAGCTGCCATTGAAATGATATGCTGA

Protein sequence

MVVSFCYHLALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC

Homology

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BLAST of Cla006632 vs. Swiss-Prot
Match: LCKB2_ARATH (Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana GN=LCKB2 PE=2 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.8e-151
Identity = 256/354 (72.32%), Postives = 298/354 (84.18%), Query Frame = 1


Query: 11  LGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVK 70
           L P  A  P   R +Q I  D +  R      +++  SSSG RDLVFVVNP+GANG+T K
Sbjct: 10  LCPFTAKQPSFLRKQQRITSDRFTFRGGGGGDAAAVVSSSGLRDLVFVVNPQGANGRTAK 69

Query: 71  EWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNG 130
           EWKKL+P+LR+RLG+  N+ E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVVNG
Sbjct: 70  EWKKLLPHLRSRLGKDCNVSELLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVVNG 129

Query: 131 FFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRIDVG 190
           FFW GKPV    GEA +STALG+IPLGTGSDFARTFGWNN+P EAV+RIA+G+RSRIDVG
Sbjct: 130 FFWEGKPVGYLSGEASRSTALGLIPLGTGSDFARTFGWNNDPCEAVERIARGMRSRIDVG 189

Query: 191 VVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVD 250
           V+++E  + HYF NVAD+HLSAKAGFYA++YKKFGNLCYVIGALQ FMGH N+D +I+V+
Sbjct: 190 VIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAFMGHHNQDMRIRVN 249

Query: 251 DGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKIYN 310
            GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NG+LEVV+LQ+FKWYDF+L LHK+YN
Sbjct: 250 GGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQNFKWYDFVLKLHKLYN 309

Query: 311 GTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 365
           GTHL+V NVTSRSV+SIEVEE++ SGSIYVQSDGEHLGFLPRKF +LP AI++I
Sbjct: 310 GTHLSVNNVTSRSVQSIEVEEITDSGSIYVQSDGEHLGFLPRKFQVLPGAIDII 363

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BLAST of Cla006632 vs. Swiss-Prot
Match: DAGK_MYCTU (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) GN=dagK PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.8e-23
Identity = 88/311 (28.30%), Postives = 139/311 (44.69%), Query Frame = 1


Query: 58  VVNPRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIA 117
           + NP   +G  VK     +  L+ R     ++ E +      A  +   A+ +G DAV+ 
Sbjct: 17  LTNPLSGHGAAVKAAHGAIARLKHR---GVDVVEIVGGDAHDARHLLAAAVAKGTDAVMV 76

Query: 118 VGGDGTFHEVVNGFFWGGKPVVNNDGEARKST--ALGIIPLGTGSDFARTFGW-NNNPFE 177
            GGDG               VV+N  +    T   LGIIP GTG+D AR FG    NP  
Sbjct: 77  TGGDG---------------VVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKA 136

Query: 178 AVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYK-KFGNLCYVIGA 237
           A D +  G    ID+G + ++NG   +F  VA     +     A R +   G + Y I  
Sbjct: 137 AADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAM 196

Query: 238 LQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVIL 297
           L      +   F++ V DG  E+   +T    GN + +GGG+ I PNA+ S+G L++ + 
Sbjct: 197 LAELSRLRPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMA 256

Query: 298 QDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRK 357
           Q       +     I+ G H+ +  V++   +++ VE       I V +DG+    LP +
Sbjct: 257 QSDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE----CPGINVYADGDFACPLPAE 304

Query: 358 FHILPAAIEMI 365
              +PAA++++
Sbjct: 317 ISAVPAALQVL 304

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BLAST of Cla006632 vs. Swiss-Prot
Match: DAGK_MYCTO (Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) GN=dagK PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 3.8e-23
Identity = 88/311 (28.30%), Postives = 139/311 (44.69%), Query Frame = 1


Query: 58  VVNPRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIA 117
           + NP   +G  VK     +  L+ R     ++ E +      A  +   A+ +G DAV+ 
Sbjct: 17  LTNPLSGHGAAVKAAHGAIARLKHR---GVDVVEIVGGDAHDARHLLAAAVAKGTDAVMV 76

Query: 118 VGGDGTFHEVVNGFFWGGKPVVNNDGEARKST--ALGIIPLGTGSDFARTFGW-NNNPFE 177
            GGDG               VV+N  +    T   LGIIP GTG+D AR FG    NP  
Sbjct: 77  TGGDG---------------VVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKA 136

Query: 178 AVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYK-KFGNLCYVIGA 237
           A D +  G    ID+G + ++NG   +F  VA     +     A R +   G + Y I  
Sbjct: 137 AADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAM 196

Query: 238 LQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVIL 297
           L      +   F++ V DG  E+   +T    GN + +GGG+ I PNA+ S+G L++ + 
Sbjct: 197 LAELSRLRPLPFRL-VLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMA 256

Query: 298 QDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRK 357
           Q       +     I+ G H+ +  V++   +++ VE       I V +DG+    LP +
Sbjct: 257 QSDSRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVE----CPGINVYADGDFACPLPAE 304

Query: 358 FHILPAAIEMI 365
              +PAA++++
Sbjct: 317 ISAVPAALQVL 304

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BLAST of Cla006632 vs. Swiss-Prot
Match: DAGK_STACT (Diacylglycerol kinase OS=Staphylococcus carnosus (strain TM300) GN=dagK PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.0e-15
Identity = 69/262 (26.34%), Postives = 117/262 (44.66%), Query Frame = 1


Query: 104 TREAIREGADAVIAVGGDGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFA 163
           ++ A++E  + +I  GGDGT +EVVNG             E  K   +G+IP+GT +DF 
Sbjct: 51  SKRALQEDYEMLIVAGGDGTLNEVVNGI-----------AEHPKRPKIGVIPMGTVNDFG 110

Query: 164 RTFGWNNNPFEAVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGF-YAARYK 223
           R      +  +AVD I +G   ++D+G +N       YF N+A      +  +  +++ K
Sbjct: 111 RALHLPTDILKAVDVIIEGHSVKVDIGKMNS-----RYFINLAAGGRITEVSYETSSKLK 170

Query: 224 KF-GNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNA 283
            F G   Y I  ++      N D +I+ D   ++   ++    +G      G  K+VP+A
Sbjct: 171 TFVGPFAYYIKGMEMLPQMTNVDVRIEYDGQVFQ--GEILLFLLGLTNSMAGFEKLVPDA 230

Query: 284 NPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQ 343
              +G   ++I+Q     +    +     G H+    V     +S+    +SS   + + 
Sbjct: 231 RLDDGYFTLIIVQKANLAELGHIMTLASRGEHIKHPKVIYEKAKSV---NISSFEQMPLN 290

Query: 344 SDGEHLGFLPRKFHILPAAIEM 364
            DGE+ G LP  F  L   IE+
Sbjct: 291 VDGEYGGQLPANFLNLKQHIEV 291

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BLAST of Cla006632 vs. Swiss-Prot
Match: DAGK_STAS1 (Diacylglycerol kinase OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229) GN=dagK PE=3 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.9e-14
Identity = 71/262 (27.10%), Postives = 113/262 (43.13%), Query Frame = 1


Query: 107 AIREGADAVIAVGGDGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTF 166
           ++ +  D +IA GGDGT +EV+NG             E     +LGIIP+GT +DF R  
Sbjct: 54  SLEQNYDVLIAAGGDGTLNEVINGI-----------AEKPNRPSLGIIPMGTVNDFGRAL 113

Query: 167 GWNNNPFEAVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGN 226
               +   A+D I +G  +R+D+G +N       YF     I+L+A        Y+    
Sbjct: 114 HLPTDIMSAIDVIIEGHMTRVDIGKMNS-----RYF-----INLAAGGQLTQVSYETPSK 173

Query: 227 LCYVIGA----LQGF-MGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNA 286
           L  ++G     ++GF M  Q K   I+++  +     +     +G      G  K+VP+A
Sbjct: 174 LKSIVGPFAYYIKGFEMLPQMKAVDIRIEYDDEVFQGEALLFLLGLTNSMAGFEKLVPDA 233

Query: 287 NPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQ 346
              +G   ++I++     +    +     G H     V  +  +SI    VSS   + + 
Sbjct: 234 KLDDGYFTLIIVEKANLAELGHIMTLASRGEHTKHPKVHYKKAKSI---SVSSFTDMQLN 291

Query: 347 SDGEHLGFLPRKFHILPAAIEM 364
            DGE+ G LP  F  L   IE+
Sbjct: 294 VDGEYGGKLPGNFLNLKQHIEV 291

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BLAST of Cla006632 vs. TrEMBL
Match: M5WZY9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007544mg PE=4 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 1.8e-157
Identity = 273/347 (78.67%), Postives = 303/347 (87.32%), Query Frame = 1


Query: 19  PFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVKEWKKLVPY 78
           P+  RAE P APDL ADRS    GSSSF     RRDLVFVVNP GANG+T KEWKKL+PY
Sbjct: 22  PWAVRAELPKAPDLCADRSFLRSGSSSFR----RRDLVFVVNPSGANGRTGKEWKKLLPY 81

Query: 79  LRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWGGKPV 138
           LR+RLG   N+CESLTSGP HAIDITREAIREGADAVI VGGDGT HEVVNGFFW GKPV
Sbjct: 82  LRSRLGADCNLCESLTSGPSHAIDITREAIREGADAVIVVGGDGTLHEVVNGFFWAGKPV 141

Query: 139 VNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRIDVGVVNEENGE 198
            N+D E   STALG+IPLGTGSDFARTFGW N+P EA+DRIAKG RSRIDVGV++ E+GE
Sbjct: 142 TNHDREVTHSTALGLIPLGTGSDFARTFGWKNDPHEAIDRIAKGQRSRIDVGVISGEDGE 201

Query: 199 YHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYP 258
            HYF NVADIHLSAKAG+YA+RYK+FGNLCYVIGAL+ F+GH+N+D KIKV++GEWELY 
Sbjct: 202 PHYFVNVADIHLSAKAGYYASRYKRFGNLCYVIGALKAFVGHRNQDLKIKVNEGEWELYS 261

Query: 259 QVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKN 318
           QVTALCIGNAK+FGGGMKI PNA+P +G+ EVVILQDFKWYDFIL LHK+YNGTHLTVKN
Sbjct: 262 QVTALCIGNAKFFGGGMKITPNADPHSGNFEVVILQDFKWYDFILKLHKLYNGTHLTVKN 321

Query: 319 VTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC 366
           V+SRSV SIEVE+VS SGSIYVQSDGEHLGFLPRKF ILP+A+EMIC
Sbjct: 322 VSSRSVHSIEVEDVSGSGSIYVQSDGEHLGFLPRKFCILPSAVEMIC 364

[Close]

BLAST of Cla006632 vs. TrEMBL
Match: A0A059CJK2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02318 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 3.8e-155
Identity = 275/371 (74.12%), Postives = 316/371 (85.18%), Query Frame = 1


Query: 3   VSFCYH-LALG---PS-MATTPFITRAEQ-PIAP--DLYADRSIFHRGSSSFSSSSGRRD 62
           + +CY+ +ALG   PS MA +  + RAEQ P+AP  DL  DR++   GS   S+S GRRD
Sbjct: 1   MGWCYYQVALGRAPPSLMANSSVVARAEQRPMAPPPDLPFDRTLLRGGS--LSASRGRRD 60

Query: 63  LVFVVNPRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADA 122
           LVFVVNPRGANGKT KEWKKL+PYLR+RLGE  NICES TSGPCHA DITRE+IREGADA
Sbjct: 61  LVFVVNPRGANGKTWKEWKKLLPYLRSRLGEDCNICESFTSGPCHATDITRESIREGADA 120

Query: 123 VIAVGGDGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFE 182
           VIAVGGDGT HEVVNGFFW GKPV N D     STALG+IPLGTGSDFARTFGW N+P +
Sbjct: 121 VIAVGGDGTLHEVVNGFFWAGKPVTNQDTATSCSTALGLIPLGTGSDFARTFGWKNDPHD 180

Query: 183 AVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGAL 242
           A++RIAKG RS+IDVGV+  EN   HYF NVADIHLSAKAG+YA+RYK+FGNLCYV GAL
Sbjct: 181 AIERIAKGQRSQIDVGVIVGENEHRHYFLNVADIHLSAKAGYYASRYKRFGNLCYVFGAL 240

Query: 243 QGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQ 302
           Q F+GH+N+DF+IKV+DGEWE YPQVTALCIGNAK+FGGGMKI PNA+PS+G+ EVVILQ
Sbjct: 241 QAFIGHRNQDFRIKVNDGEWETYPQVTALCIGNAKFFGGGMKITPNADPSSGNFEVVILQ 300

Query: 303 DFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKF 362
           DF+WYDFIL LHK+YNGTHL+V NV+SRSV SIEVEE+S +GSI+VQSDGEHLGFLPRKF
Sbjct: 301 DFRWYDFILKLHKLYNGTHLSVNNVSSRSVHSIEVEEISGTGSIFVQSDGEHLGFLPRKF 360

Query: 363 HILPAAIEMIC 366
            +LP+AIEMIC
Sbjct: 361 RVLPSAIEMIC 369

[Close]

BLAST of Cla006632 vs. TrEMBL
Match: D7LDW2_ARALL (Diacylglycerol kinase family protein (Fragment) OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_483755 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.4e-152
Identity = 262/357 (73.39%), Postives = 302/357 (84.59%), Query Frame = 1


Query: 9   LALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKT 68
           + L P  A  P   R +Q I  D +  R      +S+  SSSG R+LVFVVNP+GANG+T
Sbjct: 10  IPLCPFTAKQPSFPRKQQRITSDHFTLRGGGGGSASAVVSSSGLRELVFVVNPQGANGRT 69

Query: 69  VKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVV 128
            KEWKKL+PYLR+RLG+  N+ ESLTSGP HAIDITREAIR+GADAVIAVGGDGT HEVV
Sbjct: 70  AKEWKKLLPYLRSRLGKDCNVSESLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVV 129

Query: 129 NGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRID 188
           NGFFW GKPV N + EA  STALG+IPLGTGSDFARTFGWNN+P EAV+RIAKG+RSR+D
Sbjct: 130 NGFFWEGKPVGNLNSEASHSTALGLIPLGTGSDFARTFGWNNDPCEAVERIAKGMRSRVD 189

Query: 189 VGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIK 248
           VG++++E  + HYF NVAD+HLSAKAGFYA++YKKFGNLCYVIGALQ FMGH N+D +I+
Sbjct: 190 VGIIDKEGKDLHYFINVADVHLSAKAGFYASKYKKFGNLCYVIGALQAFMGHHNRDMRIR 249

Query: 249 VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKI 308
           V+ GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NG+LEVV+LQDFKWYDF+L LHK+
Sbjct: 250 VNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAIPGNGNLEVVVLQDFKWYDFVLKLHKL 309

Query: 309 YNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC 366
           YNGTHL+V NVTSRSV SIEVEE+S SGSIYVQSDGEHLGFLPRKF ILP AI+MIC
Sbjct: 310 YNGTHLSVNNVTSRSVLSIEVEEISDSGSIYVQSDGEHLGFLPRKFRILPGAIDMIC 366

[Close]

BLAST of Cla006632 vs. TrEMBL
Match: R0G0Y8_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10025121mg PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 8.8e-152
Identity = 259/356 (72.75%), Postives = 303/356 (85.11%), Query Frame = 1


Query: 9   LALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKT 68
           +++ P  A  P   R +Q I  DL + R+    G ++  SSS  RDLVFVVNP+GANG+T
Sbjct: 8   VSVSPFTAKQPSFIRTQQRITSDLSSGRNPLRGGGAAAVSSSSLRDLVFVVNPQGANGRT 67

Query: 69  VKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVV 128
            KEWKKL+PYLR+RLG+  NICESLTSGP HAIDITREAIR+GADAVIAVGGDGT HEVV
Sbjct: 68  AKEWKKLLPYLRSRLGKDCNICESLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVV 127

Query: 129 NGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRID 188
           NGFFW GKPV N + EA  STALG+IPLGTGSDFARTFGW+N+P EAV+RIAKG+RS++D
Sbjct: 128 NGFFWEGKPVSNLNSEASHSTALGLIPLGTGSDFARTFGWSNDPCEAVERIAKGMRSQVD 187

Query: 189 VGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIK 248
           VGV+++E  + HYF NVAD+HLSAKAGFYA++YKKFG LCYVIGALQ FMGH N+D +I+
Sbjct: 188 VGVIDKEGRDLHYFINVADVHLSAKAGFYASKYKKFGKLCYVIGALQAFMGHHNRDLRIR 247

Query: 249 VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKI 308
           V+ GEWELYPQVTALC+GNAKYFGGGMKI PNA P +G+LEVV+LQDFKWYDF+L LHK+
Sbjct: 248 VNGGEWELYPQVTALCVGNAKYFGGGMKITPNAVPGSGNLEVVVLQDFKWYDFVLKLHKL 307

Query: 309 YNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 365
           YNGTHL+V NVTSRSV+SIEVEE+S SGSIYVQSDGEHLGFLP KF +LP AI+MI
Sbjct: 308 YNGTHLSVNNVTSRSVQSIEVEEISGSGSIYVQSDGEHLGFLPGKFWVLPGAIDMI 363

[Close]

BLAST of Cla006632 vs. TrEMBL
Match: A0A078CD69_BRANA (BnaA05g05360D protein OS=Brassica napus GN=BnaA05g05360D PE=4 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.2e-151
Identity = 262/356 (73.60%), Postives = 298/356 (83.71%), Query Frame = 1


Query: 9   LALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKT 68
           +++ P  A  P   R    I+  L + R   H G S+  S S  RDLVFVVNP+GANG+T
Sbjct: 8   ISIRPYTAKQPSFHRLHHSISSSLSSGRITLHGGGSAAVSPSRLRDLVFVVNPQGANGRT 67

Query: 69  VKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVV 128
            KEWKKL+PYLRTRLG+  NI E LTSGP HAIDITREAIR+GADAVIAVGGDGT HEVV
Sbjct: 68  AKEWKKLLPYLRTRLGKDCNIKEYLTSGPSHAIDITREAIRDGADAVIAVGGDGTLHEVV 127

Query: 129 NGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRID 188
           NGFFW GKPV N DGEA  S ALG+IPLGTGSDFARTFGW N+P EAV+RIAKG+RSRID
Sbjct: 128 NGFFWEGKPVSNLDGEASHSAALGLIPLGTGSDFARTFGWRNDPCEAVERIAKGVRSRID 187

Query: 189 VGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIK 248
           VGV++ E  + HYF NVAD+HLSAKAGFYA++YKKFG+LCYVIGALQ FMGH N+D +IK
Sbjct: 188 VGVIDHEESDLHYFINVADVHLSAKAGFYASKYKKFGSLCYVIGALQAFMGHHNRDMRIK 247

Query: 249 VDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKI 308
           V+ GEWE+YPQVTALC+GNAKYFGGGMKI PNA P NG+LEVV+LQDFKWYDFIL LHK+
Sbjct: 248 VNGGEWEIYPQVTALCVGNAKYFGGGMKITPNAVPGNGNLEVVVLQDFKWYDFILKLHKL 307

Query: 309 YNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMI 365
           YNGTHL+V NV+SRSV+SIE+EEVS SGSIYVQSDGEHLGFLPRKF +LP AI+MI
Sbjct: 308 YNGTHLSVNNVSSRSVQSIEIEEVSESGSIYVQSDGEHLGFLPRKFQVLPGAIDMI 363

[Close]

BLAST of Cla006632 vs. NCBI nr
Match: gi|659116626|ref|XP_008458171.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 716.8 bits (1849), Expect = 1.9e-203
Identity = 346/365 (94.79%), Postives = 354/365 (96.99%), Query Frame = 1


Query: 1   MVVSFCYHLALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALG SMAT PFIT AEQP APDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT KEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA 180
           DGTFHEVVNGFFW GKP+VNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA 181

Query: 181 KGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGH 240
           KGLR+RIDVGVVNE+NGE HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGH
Sbjct: 182 KGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGH 241

Query: 241 QNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYD 300
           QN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNA+PSNGSLEVVILQDFKWYD
Sbjct: 242 QNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVILQDFKWYD 301

Query: 301 FILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAA 360
           FILNLHKIYNGTHLTVKNVTS+S+RSIEVEEVS SGSIYVQSDGEHLGFLPRKFHILPAA
Sbjct: 302 FILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRKFHILPAA 361

Query: 361 IEMIC 366
           IEMIC
Sbjct: 362 IEMIC 366

[Close]

BLAST of Cla006632 vs. NCBI nr
Match: gi|659116624|ref|XP_008458170.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 709.9 bits (1831), Expect = 2.3e-201
Identity = 346/372 (93.01%), Postives = 354/372 (95.16%), Query Frame = 1


Query: 1   MVVSFCYHLALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALG SMAT PFIT AEQP APDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGSSMATKPFITMAEQPTAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT KEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWGGKPVVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA 180
           DGTFHEVVNGFFW GKP+VNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIA 181

Query: 181 K-------GLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGA 240
           K       GLR+RIDVGVVNE+NGE HYFANVADIHLSAKAGFYAARYKKFGNLCYVIGA
Sbjct: 182 KVHVRYSAGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGA 241

Query: 241 LQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVIL 300
           LQGFMGHQN+DFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNA+PSNGSLEVVIL
Sbjct: 242 LQGFMGHQNRDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNADPSNGSLEVVIL 301

Query: 301 QDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRK 360
           QDFKWYDFILNLHKIYNGTHLTVKNVTS+S+RSIEVEEVS SGSIYVQSDGEHLGFLPRK
Sbjct: 302 QDFKWYDFILNLHKIYNGTHLTVKNVTSKSIRSIEVEEVSCSGSIYVQSDGEHLGFLPRK 361

Query: 361 FHILPAAIEMIC 366
           FHILPAAIEMIC
Sbjct: 362 FHILPAAIEMIC 373

[Close]

BLAST of Cla006632 vs. NCBI nr
Match: gi|778721950|ref|XP_011658380.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus])

HSP 1 Score: 647.1 bits (1668), Expect = 1.8e-182
Identity = 324/381 (85.04%), Postives = 335/381 (87.93%), Query Frame = 1


Query: 1   MVVSFCYHLALGPSMATTPFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVN 60
           +VVS CYHLALGPSMAT PFIT AEQPIAPDLY DRS FHRGSSSFSSSSGRRDLVFVVN
Sbjct: 2   VVVSLCYHLALGPSMATKPFITMAEQPIAPDLYVDRSTFHRGSSSFSSSSGRRDLVFVVN 61

Query: 61  PRGANGKTVKEWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 120
           PRGANGKT K+WKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG
Sbjct: 62  PRGANGKTAKQWKKLVPYLRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGG 121

Query: 121 DGTFHEVVNGFFWGGKPVVNNDGEARKSTALGI------------IPLGTGSDFARTFGW 180
           DGTFHEVVNGFFW GKP+VNNDGEARKSTALG+             PL T    A     
Sbjct: 122 DGTFHEVVNGFFWAGKPIVNNDGEARKSTALGVRDSFIVTILSCLFPLSTSCPMAA---- 181

Query: 181 NNNP----FEAVDRIAKGLRSRIDVGVVNEENGEYHYFANVADIHLSAKAGFYAARYKKF 240
              P       +  +AKGLR+RIDVGVVNE+NGE HYFANVADIHLSAKAGFYAARYKKF
Sbjct: 182 --KPCVLMLITLSCMAKGLRTRIDVGVVNEDNGENHYFANVADIHLSAKAGFYAARYKKF 241

Query: 241 GNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNANPS 300
           GNLCYVIGALQGFMGHQN+DF+IKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPN +PS
Sbjct: 242 GNLCYVIGALQGFMGHQNRDFRIKVDDGEWELYPQVTALCIGNAKYFGGGMKIVPNVDPS 301

Query: 301 NGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSSSGSIYVQSDG 360
           N SLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVS SGSIYVQSDG
Sbjct: 302 NRSLEVVILQDFKWYDFILNLHKIYNGTHLTVKNVTSRSVRSIEVEEVSCSGSIYVQSDG 361

Query: 361 EHLGFLPRKFHILPAAIEMIC 366
           EHLGFLPRKFHILPAAIEMIC
Sbjct: 362 EHLGFLPRKFHILPAAIEMIC 376

[Close]

BLAST of Cla006632 vs. NCBI nr
Match: gi|645254327|ref|XP_008232990.1| (PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Prunus mume])

HSP 1 Score: 564.7 bits (1454), Expect = 1.2e-157
Identity = 275/347 (79.25%), Postives = 304/347 (87.61%), Query Frame = 1


Query: 19  PFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVKEWKKLVPY 78
           P+  RAE P APDL ADRSI   G    SSSS RRDLVFVVNP GANG+T KEWKKL+PY
Sbjct: 22  PWAVRAELPRAPDLCADRSILRSG----SSSSRRRDLVFVVNPSGANGRTGKEWKKLLPY 81

Query: 79  LRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWGGKPV 138
           LR+RLG   NICESLTSGP HAIDITREAIREGADAVI VGGDGT HEVVNGFFW GKPV
Sbjct: 82  LRSRLGADCNICESLTSGPSHAIDITREAIREGADAVIVVGGDGTLHEVVNGFFWAGKPV 141

Query: 139 VNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRIDVGVVNEENGE 198
            N+D E   STALG+IPLGTGSDFARTFGW N+P EA+DRIAKG RSRIDVGV++ E+GE
Sbjct: 142 TNHDREVTHSTALGLIPLGTGSDFARTFGWKNDPHEAIDRIAKGQRSRIDVGVISGEDGE 201

Query: 199 YHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYP 258
            HYF NVADIHLSAKAG+YA+RYK+FGNLCYVIGAL+ F+GH+N+D KIKV++GEWELY 
Sbjct: 202 PHYFVNVADIHLSAKAGYYASRYKRFGNLCYVIGALKAFVGHRNQDLKIKVNEGEWELYS 261

Query: 259 QVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKN 318
           QVTALCIGNAK+FGGGMKI PNA+P +G+ EVVILQDFKWYDFIL LHK+YNGTHLTVKN
Sbjct: 262 QVTALCIGNAKFFGGGMKITPNADPHSGNFEVVILQDFKWYDFILKLHKLYNGTHLTVKN 321

Query: 319 VTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC 366
           V+SRSV SIEVE+VS SGSIYVQSDGEHLGFLPRKF ILP+A+EMIC
Sbjct: 322 VSSRSVHSIEVEDVSGSGSIYVQSDGEHLGFLPRKFCILPSAVEMIC 364

[Close]

BLAST of Cla006632 vs. NCBI nr
Match: gi|596001486|ref|XP_007218173.1| (hypothetical protein PRUPE_ppa007544mg [Prunus persica])

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-157
Identity = 273/347 (78.67%), Postives = 303/347 (87.32%), Query Frame = 1


Query: 19  PFITRAEQPIAPDLYADRSIFHRGSSSFSSSSGRRDLVFVVNPRGANGKTVKEWKKLVPY 78
           P+  RAE P APDL ADRS    GSSSF     RRDLVFVVNP GANG+T KEWKKL+PY
Sbjct: 22  PWAVRAELPKAPDLCADRSFLRSGSSSFR----RRDLVFVVNPSGANGRTGKEWKKLLPY 81

Query: 79  LRTRLGEHYNICESLTSGPCHAIDITREAIREGADAVIAVGGDGTFHEVVNGFFWGGKPV 138
           LR+RLG   N+CESLTSGP HAIDITREAIREGADAVI VGGDGT HEVVNGFFW GKPV
Sbjct: 82  LRSRLGADCNLCESLTSGPSHAIDITREAIREGADAVIVVGGDGTLHEVVNGFFWAGKPV 141

Query: 139 VNNDGEARKSTALGIIPLGTGSDFARTFGWNNNPFEAVDRIAKGLRSRIDVGVVNEENGE 198
            N+D E   STALG+IPLGTGSDFARTFGW N+P EA+DRIAKG RSRIDVGV++ E+GE
Sbjct: 142 TNHDREVTHSTALGLIPLGTGSDFARTFGWKNDPHEAIDRIAKGQRSRIDVGVISGEDGE 201

Query: 199 YHYFANVADIHLSAKAGFYAARYKKFGNLCYVIGALQGFMGHQNKDFKIKVDDGEWELYP 258
            HYF NVADIHLSAKAG+YA+RYK+FGNLCYVIGAL+ F+GH+N+D KIKV++GEWELY 
Sbjct: 202 PHYFVNVADIHLSAKAGYYASRYKRFGNLCYVIGALKAFVGHRNQDLKIKVNEGEWELYS 261

Query: 259 QVTALCIGNAKYFGGGMKIVPNANPSNGSLEVVILQDFKWYDFILNLHKIYNGTHLTVKN 318
           QVTALCIGNAK+FGGGMKI PNA+P +G+ EVVILQDFKWYDFIL LHK+YNGTHLTVKN
Sbjct: 262 QVTALCIGNAKFFGGGMKITPNADPHSGNFEVVILQDFKWYDFILKLHKLYNGTHLTVKN 321

Query: 319 VTSRSVRSIEVEEVSSSGSIYVQSDGEHLGFLPRKFHILPAAIEMIC 366
           V+SRSV SIEVE+VS SGSIYVQSDGEHLGFLPRKF ILP+A+EMIC
Sbjct: 322 VSSRSVHSIEVEDVSGSGSIYVQSDGEHLGFLPRKFCILPSAVEMIC 364

The following BLAST results are available for this feature:

BLAST of Cla006632 vs. Swiss-Prot


Match Name	E-value	Identity	Description
LCKB2_ARATH	4.8e-151	72.32	Sphingoid long-chain bases kinase 2, mitochondrial OS=Arabidopsis thaliana GN=LC...	[more]
DAGK_MYCTU	3.8e-23	28.30	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) ...	[more]
DAGK_MYCTO	3.8e-23	28.30	Diacylglycerol kinase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) ...	[more]
DAGK_STACT	5.0e-15	26.34	Diacylglycerol kinase OS=Staphylococcus carnosus (strain TM300) GN=dagK PE=3 SV=...	[more]
DAGK_STAS1	1.9e-14	27.10	Diacylglycerol kinase OS=Staphylococcus saprophyticus subsp. saprophyticus (stra...	[more]

BLAST of Cla006632 vs. TrEMBL


Match Name	E-value	Identity	Description
M5WZY9_PRUPE	1.8e-157	78.67	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007544mg PE=4 SV=1	[more]
A0A059CJK2_EUCGR	3.8e-155	74.12	Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_D02318 PE=4 SV=1	[more]
D7LDW2_ARALL	1.4e-152	73.39	Diacylglycerol kinase family protein (Fragment) OS=Arabidopsis lyrata subsp. lyr...	[more]
R0G0Y8_9BRAS	8.8e-152	72.75	Uncharacterized protein OS=Capsella rubella GN=CARUB_v10025121mg PE=4 SV=1	[more]
A0A078CD69_BRANA	1.2e-151	73.60	BnaA05g05360D protein OS=Brassica napus GN=BnaA05g05360D PE=4 SV=1	[more]

BLAST of Cla006632 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659116626\|ref\|XP_008458171.1\|	1.9e-203	94.79	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X2 [Cucumi...	[more]
gi\|659116624\|ref\|XP_008458170.1\|	2.3e-201	93.01	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial isoform X1 [Cucumi...	[more]
gi\|778721950\|ref\|XP_011658380.1\|	1.8e-182	85.04	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Cucumis sativus]	[more]
gi\|645254327\|ref\|XP_008232990.1\|	1.2e-157	79.25	PREDICTED: sphingoid long-chain bases kinase 2, mitochondrial [Prunus mume]	[more]
gi\|596001486\|ref\|XP_007218173.1\|	2.6e-157	78.67	hypothetical protein PRUPE_ppa007544mg [Prunus persica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR001206	Diacylglycerol_kinase_cat_dom
IPR005218	Diacylglycerol/lipid_kinase
IPR016064	NAD/diacylglycerol_kinase_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0016301	kinase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0006671	phytosphingosine metabolic process
biological_process	GO:0009409	response to cold
biological_process	GO:0006769	nicotinamide metabolic process
biological_process	GO:0046497	nicotinate nucleotide metabolic process
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0017050	D-erythro-sphingosine kinase activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0003951	NAD+ kinase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla006632	Cla006632.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 55..191 score: 1.4
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 54..196 score: 1.
IPR001206	Diacylglycerol kinase, catalytic domain	PROFILE	PS50146	DAGK	coord: 50..196 score: 34
IPR005218	Diacylglycerol/lipid kinase	TIGRFAMs	TIGR00147	TIGR00147	coord: 55..363 score: 3.8
IPR016064	NAD kinase/diacylglycerol kinase-like domain	unknown	SSF111331	NAD kinase/diacylglycerol kinase-like	coord: 53..365 score: 7.85
None	No IPR available	PANTHER	PTHR12358	SPHINGOSINE KINASE	coord: 45..365 score: 2.7E
None	No IPR available	PANTHER	PTHR12358:SF43	SPHINGOID LONG-CHAIN BASES KINASE 2, MITOCHONDRIAL	coord: 45..365 score: 2.7E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla006632	Cla97C06G112810	Watermelon (97103) v2	wmwmbB408
Cla006632	Csa6G006770	Cucumber (Chinese Long) v2	cuwmB540
Cla006632	MELO3C021184	Melon (DHL92) v3.5.1	mewmB137
Cla006632	ClCG06G003440	Watermelon (Charleston Gray)	wcgwmB346
Cla006632	CSPI06G00780	Wild cucumber (PI 183967)	cpiwmB559
Cla006632	Cucsa.135750	Cucumber (Gy14) v1	cgywmB236
Cla006632	CmaCh08G008870	Cucurbita maxima (Rimu)	cmawmB880
Cla006632	CmoCh08G008600	Cucurbita moschata (Rifu)	cmowmB869
Cla006632	Lsi09G015930	Bottle gourd (USVL1VR-Ls)	lsiwmB036
Cla006632	Cp4.1LG17g03790	Cucurbita pepo (Zucchini)	cpewmB335
Cla006632	CsGy6G000850	Cucumber (Gy14) v2	cgybwmB504
Cla006632	MELO3C021184.2	Melon (DHL92) v3.6.1	medwmB134
Cla006632	Carg13845	Silver-seed gourd	carwmB0601
Cla006632	CsaV3_6G000870	Cucumber (Chinese Long) v3	cucwmB565
Cla006632	Bhi12G001690	Wax gourd	wgowmB100

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla006632	Cucurbita moschata (Rifu)	cmowmB346
Cla006632	Cucurbita pepo (Zucchini)	cpewmB155
Cla006632	Watermelon (97103) v1	wmwmB151
Cla006632	Cucurbita maxima (Rimu)	cmawmB351