Cla005653 (gene) Watermelon (97103) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cla005653
Type	gene
Organism	Citrullus. lanatus (Watermelon (97103) v1)
Description	Germacrene D synthase (AHRD V1 ***- F8SME6_CITHY); contains Interpro domain(s) IPR005630 Terpene synthase, metal-binding domain
Location	Chr10 : 3144530 .. 3145900 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGGCAGGCTAGAAATTACTTTTATGAAGCAAAATGGTATAGAGAAGGGTATACACCAACAATAGAGGAGTACTTAAGGGTTGGAAGAGTAACTGCTTGCTACTCATTGTTTTCTCCCATTTCATTTCTTGGTATGGGAGATGTTGCTTCAATAGAGGCCTTTGAATGGATAGAGAGTGACCCCAAATCGCTTATTGGCTCAGGAGTAATTGGTAGGATCATGAACGATATTACTTCCCACAAGGTAAATTCTTGCATTTTTTTTTTTTTAATCATGATTCATACAATCTTTTATCTTATAATTGTTGAACTTTAAAGAAAAAATATTTTGAATGTTTAGGTTAAGTTATCGTTTAGTATGTTGTTAACGATTGAGTTTAAATCTTTATACTATTATTTTTGCATTATTTAATGTTCAAATTCCTATTCTATTATTATATTCTTTGATCATTTATCAATAATAATACATGTATATATCATAACTCAATAGTAGTTTATTGAATACACATGCACATGCATGGTTAAATTTTTACTCTAGCTCAAATATTCCCAATATATATAATGGATCAAAAGGTGATACGGTCCAAATCAATCTCACCCTACTACAAATTGATGAACTATCTCTTATAAACACTACTTTTTACCTTAGTTTTCTTTATTTTGTTATCCATTTTTTAGAATTTTTTTTTAATTTTATTTTTTAAAGTTAGCTATGATTTCAAATGATTCCTTAGGAAAAATGAAAATAATTGTAACTAAACTTAATGGTGAGAAAACAAGCATAATTTAAAAAAATTTAAATCATGTTCTCAAATGAAGTCTTGATTATCCAAGCAAGAAAGTCAACAATAGAGAGCATTACTCAACTAGCATAAACCTATACTATCAACCTCGAGGTCTAAGGTTTAATCTCTCTCACCCACCCCACATATCATTACAACACAAAATAATTAAACACTAGTAAACCCTACCTAAGATCAAGCAACGGAAAGATGTCAATACCATTAAAAAAATGAGGGTATGAAGATTTGAACATATTTTTGTCTCTAACTGTTTGTTTGATGTATAGTTCGAACAAGAAAGAGGCCATGTCGCTTCCGCAGTTGAATGTTATATGATACAGTATGATGTATCAGAAGAAGAGGCAATACAAGAATTAGAGAAGCAAGTTACAAATGCTTGGAAGGATATCATTGAAGATTATGTGCAATCTATTGAGGATGTTCCAAACACTATTTTAATGAGTGTTATAAATCTTGCTCGACTTTCAGACACCTTCTATAAAGATGAAGATGGATATACATACTCAAGTGGAGAAACCAAACGTATTGTCCTATCTTTAGTCGTTGATCCACTACCAATTTGA

mRNA sequence

ATGAAAAGGCAGGCTAGAAATTACTTTTATGAAGCAAAATGGTATAGAGAAGGGTATACACCAACAATAGAGGAGTACTTAAGGGTTGGAAGAGTAACTGCTTGCTACTCATTGTTTTCTCCCATTTCATTTCTTGGTATGGGAGATGTTGCTTCAATAGAGGCCTTTGAATGGATAGAGAGTGACCCCAAATCGCTTATTGGCTCAGGAGTAATTGGTAGGATCATGAACGATATTACTTCCCACAAGTTCGAACAAGAAAGAGGCCATGTCGCTTCCGCAGTTGAATGTTATATGATACAGTATGATGTATCAGAAGAAGAGGCAATACAAGAATTAGAGAAGCAAGTTACAAATGCTTGGAAGGATATCATTGAAGATTATGTGCAATCTATTGAGGATGTTCCAAACACTATTTTAATGAGTGTTATAAATCTTGCTCGACTTTCAGACACCTTCTATAAAGATGAAGATGGATATACATACTCAAGTGGAGAAACCAAACGTATTGTCCTATCTTTAGTCGTTGATCCACTACCAATTTGA

Coding sequence (CDS)

ATGAAAAGGCAGGCTAGAAATTACTTTTATGAAGCAAAATGGTATAGAGAAGGGTATACACCAACAATAGAGGAGTACTTAAGGGTTGGAAGAGTAACTGCTTGCTACTCATTGTTTTCTCCCATTTCATTTCTTGGTATGGGAGATGTTGCTTCAATAGAGGCCTTTGAATGGATAGAGAGTGACCCCAAATCGCTTATTGGCTCAGGAGTAATTGGTAGGATCATGAACGATATTACTTCCCACAAGTTCGAACAAGAAAGAGGCCATGTCGCTTCCGCAGTTGAATGTTATATGATACAGTATGATGTATCAGAAGAAGAGGCAATACAAGAATTAGAGAAGCAAGTTACAAATGCTTGGAAGGATATCATTGAAGATTATGTGCAATCTATTGAGGATGTTCCAAACACTATTTTAATGAGTGTTATAAATCTTGCTCGACTTTCAGACACCTTCTATAAAGATGAAGATGGATATACATACTCAAGTGGAGAAACCAAACGTATTGTCCTATCTTTAGTCGTTGATCCACTACCAATTTGA

Protein sequence

MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIESDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNAWKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLPI

Homology

[Close]

BLAST of Cla005653 vs. Swiss-Prot
Match: SAUST_SANAS (Probable sesquiterpene synthase OS=Santalum austrocaledonicum GN=SesquiTPS PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 3.0e-45
Identity = 88/179 (49.16%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MK+    Y  EAKW  E Y PT +EY+ V  VT+ Y+  + IS+LGMG++AS EAF+W+ 
Sbjct: 383 MKKLVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           S P  +  S  +GR+M+D  SHKFEQERGHVAS +ECYM QY V+EEEA  E  K++  A
Sbjct: 443 SHPPVIEASESVGRLMDDTRSHKFEQERGHVASGIECYMKQYGVTEEEARDEFRKRLVKA 502

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           WKDI E+ ++    VP  +LM ++NL R+ D  YK+EDGYT+     K  + SL++DP+
Sbjct: 503 WKDINEECLRPYR-VPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPM 560

[Close]

BLAST of Cla005653 vs. Swiss-Prot
Match: STPS1_SANAL (Sesquiterpene synthase OS=Santalum album PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.9e-45
Identity = 89/179 (49.72%), Postives = 118/179 (65.92%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MK+    Y  EAKW  E Y PT EEY+ V  VT+ Y+  + IS+LGMG++AS EAF+W+ 
Sbjct: 380 MKKLVEGYMDEAKWCHEKYVPTFEEYMPVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 439

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           S P  +  S  + R+M+D+ SHKFEQERGHVAS +ECYM QY V+EEEA  E  KQ+  A
Sbjct: 440 SHPPVIEASESVCRLMDDMRSHKFEQERGHVASGIECYMKQYGVTEEEAHDEFRKQLVKA 499

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           WKDI E+ ++    VP  +LM ++NL R+ D  YK+EDGYT+     K  + SL++DP+
Sbjct: 500 WKDINEECLRPYR-VPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPM 557

[Close]

BLAST of Cla005653 vs. Swiss-Prot
Match: SAST_SANAL (Probable sesquiterpene synthase OS=Santalum album GN=SesquiTPS1 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 6.7e-45
Identity = 87/179 (48.60%), Postives = 119/179 (66.48%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MK+    Y  EAKW  E Y PT EEY+ V  VT+ Y+  + +S+LGMG++AS EAF+W+ 
Sbjct: 380 MKKLVEGYMDEAKWCHEKYVPTFEEYMSVALVTSGYTFLTTMSYLGMGEIASKEAFDWLF 439

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           S P  +  S  +GR+M+D+ SHKFE+ERGHVAS +ECYM QY V+EEEA  E  K++  A
Sbjct: 440 SHPPVIEASESVGRLMDDMRSHKFERERGHVASGIECYMKQYSVTEEEAYDEFCKRLVKA 499

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           WKDI E+ ++    VP  +LM ++NL R+ D  YK+EDGYT+     K  + SL++DP+
Sbjct: 500 WKDINEECLRPYH-VPKPLLMRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPM 557

[Close]

BLAST of Cla005653 vs. Swiss-Prot
Match: TPSGD_VITVI ((-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.9e-44
Identity = 90/181 (49.72%), Postives = 119/181 (65.75%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MK Q R Y+ EAKW +    PT+EEY+ V  VT+ YS+ +  SF+GMGD  + E+F+WI 
Sbjct: 378 MKNQVRAYYEEAKWLQVQQIPTMEEYMPVALVTSAYSMLATTSFVGMGDAVTKESFDWIF 437

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           S PK +  S ++ R+M+D+  HKFEQ+RGHVASAVECYM Q+  SE+E   E  + V  A
Sbjct: 438 SKPKIVRASAIVCRLMDDMVFHKFEQKRGHVASAVECYMKQHGASEQETPNEFPQPVREA 497

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKDI E+ +     VP  ILM V+NLAR+ D  YK+EDGYT+     K  V S+++DP+P
Sbjct: 498 WKDINEECLIPTA-VPMPILMRVLNLARVIDVIYKNEDGYTHFGAVLKDFVTSMLIDPVP 557

Query: 181 I 182
           I
Sbjct: 558 I 557

[Close]

BLAST of Cla005653 vs. Swiss-Prot
Match: SMST_SANMU (Probable sesquiterpene synthase OS=Santalum murrayanum GN=STPS PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.3e-44
Identity = 87/179 (48.60%), Postives = 117/179 (65.36%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MK     Y  EAKW  E Y PT +EY+ V  VT+ Y+  + IS+LGMG++AS EAF+W+ 
Sbjct: 383 MKTIVEGYMDEAKWCHEKYVPTFQEYMSVALVTSGYTFLTTISYLGMGEIASKEAFDWLF 442

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           S P  +  S  +GR+M+D+ SHKFEQERGHVAS +ECYM QY V+EEEA  E  K++  A
Sbjct: 443 SHPPVIEASESVGRLMDDMRSHKFEQERGHVASGIECYMKQYGVTEEEAHDEFRKRLVKA 502

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           WKDI E+ ++    VP  +L  ++NL R+ D  YK+EDGYT+     K  + SL++DP+
Sbjct: 503 WKDINEECLRPYR-VPKPLLTRILNLTRVIDVIYKNEDGYTHVKKAMKDNIASLLIDPV 560

[Close]

BLAST of Cla005653 vs. TrEMBL
Match: A0A0A0L6X1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G021130 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 3.5e-85
Identity = 157/182 (86.26%), Postives = 173/182 (95.05%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQARNYF+EAKWY+E YTPTIEEYLRVGR+TACYSLFSPISFLGMG+VASIEAFEWIE
Sbjct: 372 MKRQARNYFFEAKWYKEEYTPTIEEYLRVGRITACYSLFSPISFLGMGNVASIEAFEWIE 431

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQE-LEKQVTN 120
           SDPKSLIG+GVIGRIMNDI SHKFEQERGHVASAVECYM+QY+V E+EAI+E LEKQV N
Sbjct: 432 SDPKSLIGAGVIGRIMNDIVSHKFEQERGHVASAVECYMMQYNVLEKEAIRELLEKQVAN 491

Query: 121 AWKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           AWK+IIEDYV+SIEDVPNTI  SV+NLARLS+TFYKDEDGYT S GETKRIV+SLV+DP+
Sbjct: 492 AWKNIIEDYVESIEDVPNTIFKSVLNLARLSETFYKDEDGYTCSDGETKRIVISLVLDPI 551

Query: 181 PI 182
           PI
Sbjct: 552 PI 553

[Close]

BLAST of Cla005653 vs. TrEMBL
Match: A0A0A0L1X6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 3.8e-63
Identity = 114/181 (62.98%), Postives = 142/181 (78.45%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT EEY++V  V+ CY LF PISF+GMG  AS EAFEW+E
Sbjct: 382 MKRQCRVYFEEAKWFHEGYVPTFEEYMKVAIVSTCYYLFVPISFVGMGIAASQEAFEWVE 441

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG++GR+MNDITSHK+EQ+RGH+ SAVECYM Q++VSEE+AI EL K++T A
Sbjct: 442 SDPMLLKASGIVGRLMNDITSHKYEQKRGHMDSAVECYMKQHEVSEEDAIVELGKEITKA 501

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD+IEDY+     + N ILM V+NL RLSD FYK EDGYT+  G+TK  + S+++DP+P
Sbjct: 502 WKDVIEDYIMKSTKLSNAILMRVLNLTRLSDLFYKKEDGYTFVHGDTKHFITSMLIDPIP 561

Query: 181 I 182
           I
Sbjct: 562 I 562

[Close]

BLAST of Cla005653 vs. TrEMBL
Match: A0A0A0L4K8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G041370 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.6e-61
Identity = 115/180 (63.89%), Postives = 140/180 (77.78%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT+EEY++V  V+ CY LF  ISF+GMG  AS EAF+WIE
Sbjct: 211 MKRQCRVYFEEAKWFYEGYVPTMEEYMKVAIVSTCYHLFVQISFVGMGVAASQEAFQWIE 270

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG+IGR++NDITSH+FEQ+RGH+ASAVECYM QY+VSEE A+ EL+K++  A
Sbjct: 271 SDPMLLKASGIIGRLINDITSHQFEQQRGHIASAVECYMKQYEVSEEVAVIELQKEIEKA 330

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD IEDY+       N ILM V+NLARLSD +YK EDGYT+S GETK  V  +++DPLP
Sbjct: 331 WKDGIEDYIMKSTKFSNAILMRVLNLARLSDFYYKKEDGYTFSHGETKDFVTYMLIDPLP 390

[Close]

BLAST of Cla005653 vs. TrEMBL
Match: A0A0B4VFP0_CUCME (Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 5.1e-60
Identity = 109/181 (60.22%), Postives = 139/181 (76.80%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT EEY++V  V+ C+ ++ PISF+GMG  AS E FEW+E
Sbjct: 342 MKRQCRAYFAEAKWFHEGYMPTFEEYMKVAIVSTCFYVYVPISFVGMGVAASQEVFEWVE 401

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG+IGR+MNDITSHKFEQERGH+ SAVECYM Q++VSEE+AI EL K++T A
Sbjct: 402 SDPMLLKASGIIGRLMNDITSHKFEQERGHIDSAVECYMKQHEVSEEDAIIELAKEITKA 461

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD+IEDY+     +   ILM V+N  RLS+ FYK EDGYT+  G+TK  + ++++DP+P
Sbjct: 462 WKDMIEDYIMKSTKLSTAILMRVLNHGRLSEFFYKKEDGYTFVHGDTKHFITAMLIDPIP 521

Query: 181 I 182
           I
Sbjct: 522 I 522

[Close]

BLAST of Cla005653 vs. TrEMBL
Match: A0A0A0L5V3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040860 PE=4 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 9.1e-57
Identity = 109/182 (59.89%), Postives = 141/182 (77.47%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGD-VASIEAFEWI 60
           MK Q R +F EAKW+ EGY PT+EEY++V   +A Y +F+ ISFL +G+  AS + +EW 
Sbjct: 1   MKNQCRAFFMEAKWFNEGYIPTVEEYMKVSITSAAYYVFASISFLTIGNNAASKQVYEWA 60

Query: 61  ESDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTN 120
           ++DP  L  + VIGR +NDI SHKFEQERGHVASAVECYM QY VSEEEA+ EL+KQV N
Sbjct: 61  QTDPILLRATSVIGRFVNDIASHKFEQERGHVASAVECYMKQYGVSEEEAVTELQKQVVN 120

Query: 121 AWKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           AWKDIIEDY++S +  P+ IL  V+N+ARLSD FYK+EDGYT++ GETKR +  ++ +P+
Sbjct: 121 AWKDIIEDYMKSSKLFPSFILDHVLNVARLSDLFYKEEDGYTFADGETKRFITLMLKNPM 180

Query: 181 PI 182
           PI
Sbjct: 181 PI 182

[Close]

BLAST of Cla005653 vs. NCBI nr
Match: gi|778675433|ref|XP_004147250.2| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 322.4 bits (825), Expect = 5.1e-85
Identity = 157/182 (86.26%), Postives = 173/182 (95.05%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQARNYF+EAKWY+E YTPTIEEYLRVGR+TACYSLFSPISFLGMG+VASIEAFEWIE
Sbjct: 372 MKRQARNYFFEAKWYKEEYTPTIEEYLRVGRITACYSLFSPISFLGMGNVASIEAFEWIE 431

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQE-LEKQVTN 120
           SDPKSLIG+GVIGRIMNDI SHKFEQERGHVASAVECYM+QY+V E+EAI+E LEKQV N
Sbjct: 432 SDPKSLIGAGVIGRIMNDIVSHKFEQERGHVASAVECYMMQYNVLEKEAIRELLEKQVAN 491

Query: 121 AWKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           AWK+IIEDYV+SIEDVPNTI  SV+NLARLS+TFYKDEDGYT S GETKRIV+SLV+DP+
Sbjct: 492 AWKNIIEDYVESIEDVPNTIFKSVLNLARLSETFYKDEDGYTCSDGETKRIVISLVLDPI 551

Query: 181 PI 182
           PI
Sbjct: 552 PI 553

[Close]

BLAST of Cla005653 vs. NCBI nr
Match: gi|659095987|ref|XP_008448865.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis melo])

HSP 1 Score: 319.3 bits (817), Expect = 4.3e-84
Identity = 155/182 (85.16%), Postives = 170/182 (93.41%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQAR YF+EAKWY+E YTPTIEEYLR+GRVTACYSLFSPISFLGMG+VAS EAFEWIE
Sbjct: 386 MKRQARYYFFEAKWYKEEYTPTIEEYLRIGRVTACYSLFSPISFLGMGNVASTEAFEWIE 445

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDPK+L+GSGVIGRIMNDI SHKFEQERGHVASAVECYMIQY+V E+EA QELEKQVTNA
Sbjct: 446 SDPKALVGSGVIGRIMNDIVSHKFEQERGHVASAVECYMIQYNVPEKEARQELEKQVTNA 505

Query: 121 WKDIIEDYVQSIE-DVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPL 180
           WKDIIEDY++S E DVPNTI  SV+NLARLSDTFYKDEDGYT S GETKR+++SLV+DPL
Sbjct: 506 WKDIIEDYIKSTENDVPNTIFKSVLNLARLSDTFYKDEDGYTCSGGETKRMIISLVLDPL 565

Query: 181 PI 182
           PI
Sbjct: 566 PI 567

[Close]

BLAST of Cla005653 vs. NCBI nr
Match: gi|778675684|ref|XP_011650453.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 5.4e-63
Identity = 114/181 (62.98%), Postives = 142/181 (78.45%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT EEY++V  V+ CY LF PISF+GMG  AS EAFEW+E
Sbjct: 382 MKRQCRVYFEEAKWFHEGYVPTFEEYMKVAIVSTCYYLFVPISFVGMGIAASQEAFEWVE 441

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG++GR+MNDITSHK+EQ+RGH+ SAVECYM Q++VSEE+AI EL K++T A
Sbjct: 442 SDPMLLKASGIVGRLMNDITSHKYEQKRGHMDSAVECYMKQHEVSEEDAIVELGKEITKA 501

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD+IEDY+     + N ILM V+NL RLSD FYK EDGYT+  G+TK  + S+++DP+P
Sbjct: 502 WKDVIEDYIMKSTKLSNAILMRVLNLTRLSDLFYKKEDGYTFVHGDTKHFITSMLIDPIP 561

Query: 181 I 182
           I
Sbjct: 562 I 562

[Close]

BLAST of Cla005653 vs. NCBI nr
Match: gi|778688341|ref|XP_011652723.1| (PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 5.1e-61
Identity = 115/180 (63.89%), Postives = 140/180 (77.78%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT+EEY++V  V+ CY LF  ISF+GMG  AS EAF+WIE
Sbjct: 315 MKRQCRVYFEEAKWFYEGYVPTMEEYMKVAIVSTCYHLFVQISFVGMGVAASQEAFQWIE 374

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG+IGR++NDITSH+FEQ+RGH+ASAVECYM QY+VSEE A+ EL+K++  A
Sbjct: 375 SDPMLLKASGIIGRLINDITSHQFEQQRGHIASAVECYMKQYEVSEEVAVIELQKEIEKA 434

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD IEDY+       N ILM V+NLARLSD +YK EDGYT+S GETK  V  +++DPLP
Sbjct: 435 WKDGIEDYIMKSTKFSNAILMRVLNLARLSDFYYKKEDGYTFSHGETKDFVTYMLIDPLP 494

[Close]

BLAST of Cla005653 vs. NCBI nr
Match: gi|700200836|gb|KGN55969.1| (hypothetical protein Csa_3G041370 [Cucumis sativus])

HSP 1 Score: 242.7 bits (618), Expect = 5.1e-61
Identity = 115/180 (63.89%), Postives = 140/180 (77.78%), Query Frame = 1


Query: 1   MKRQARNYFYEAKWYREGYTPTIEEYLRVGRVTACYSLFSPISFLGMGDVASIEAFEWIE 60
           MKRQ R YF EAKW+ EGY PT+EEY++V  V+ CY LF  ISF+GMG  AS EAF+WIE
Sbjct: 211 MKRQCRVYFEEAKWFYEGYVPTMEEYMKVAIVSTCYHLFVQISFVGMGVAASQEAFQWIE 270

Query: 61  SDPKSLIGSGVIGRIMNDITSHKFEQERGHVASAVECYMIQYDVSEEEAIQELEKQVTNA 120
           SDP  L  SG+IGR++NDITSH+FEQ+RGH+ASAVECYM QY+VSEE A+ EL+K++  A
Sbjct: 271 SDPMLLKASGIIGRLINDITSHQFEQQRGHIASAVECYMKQYEVSEEVAVIELQKEIEKA 330

Query: 121 WKDIIEDYVQSIEDVPNTILMSVINLARLSDTFYKDEDGYTYSSGETKRIVLSLVVDPLP 180
           WKD IEDY+       N ILM V+NLARLSD +YK EDGYT+S GETK  V  +++DPLP
Sbjct: 331 WKDGIEDYIMKSTKFSNAILMRVLNLARLSDFYYKKEDGYTFSHGETKDFVTYMLIDPLP 390

The following BLAST results are available for this feature:

BLAST of Cla005653 vs. Swiss-Prot


Match Name	E-value	Identity	Description
SAUST_SANAS	3.0e-45	49.16	Probable sesquiterpene synthase OS=Santalum austrocaledonicum GN=SesquiTPS PE=3 ...	[more]
STPS1_SANAL	3.9e-45	49.72	Sesquiterpene synthase OS=Santalum album PE=1 SV=1	[more]
SAST_SANAL	6.7e-45	48.60	Probable sesquiterpene synthase OS=Santalum album GN=SesquiTPS1 PE=3 SV=1	[more]
TPSGD_VITVI	1.9e-44	49.72	(-)-germacrene D synthase OS=Vitis vinifera GN=VIT_19s0014g04930 PE=1 SV=1	[more]
SMST_SANMU	3.3e-44	48.60	Probable sesquiterpene synthase OS=Santalum murrayanum GN=STPS PE=3 SV=1	[more]

BLAST of Cla005653 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6X1_CUCSA	3.5e-85	86.26	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G021130 PE=3 SV=1	[more]
A0A0A0L1X6_CUCSA	3.8e-63	62.98	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G039850 PE=3 SV=1	[more]
A0A0A0L4K8_CUCSA	3.6e-61	63.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G041370 PE=3 SV=1	[more]
A0A0B4VFP0_CUCME	5.1e-60	60.22	Sesquiterpene synthase Tps3 OS=Cucumis melo PE=2 SV=1	[more]
A0A0A0L5V3_CUCSA	9.1e-57	59.89	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G040860 PE=4 SV=1	[more]

BLAST of Cla005653 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778675433\|ref\|XP_004147250.2\|	5.1e-85	86.26	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|659095987\|ref\|XP_008448865.1\|	4.3e-84	85.16	PREDICTED: (-)-germacrene D synthase-like [Cucumis melo]	[more]
gi\|778675684\|ref\|XP_011650453.1\|	5.4e-63	62.98	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|778688341\|ref\|XP_011652723.1\|	5.1e-61	63.89	PREDICTED: (-)-germacrene D synthase-like [Cucumis sativus]	[more]
gi\|700200836\|gb\|KGN55969.1\|	5.1e-61	63.89	hypothetical protein Csa_3G041370 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR005630	Terpene_synthase_metal-bd
IPR008949	Isoprenoid_synthase_dom_sf

Vocabulary: **Molecular Function**
Term	Definition
GO:0000287	magnesium ion binding
GO:0010333	terpene synthase activity
GO:0016829	lyase activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010333	terpene synthase activity
molecular_function	GO:0016829	lyase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cla005653	Cla005653.1	mRNA

InterPro

Analysis Name: InterPro Annotations of watermelon (97103)
Date Performed: 2016-09-28


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005630	Terpene synthase, metal-binding domain	PFAM	PF03936	Terpene_synth_C	coord: 1..123 score: 1.6
IPR008949	Isoprenoid synthase domain	GENE3D	G3DSA:1.10.600.10		coord: 1..180 score: 1.6
IPR008949	Isoprenoid synthase domain	unknown	SSF48576	Terpenoid synthases	coord: 2..179 score: 7.19
None	No IPR available	PANTHER	PTHR31225	FAMILY NOT NAMED	coord: 1..181 score: 9.3
None	No IPR available	PANTHER	PTHR31225:SF15	ALPHA-HUMULENE/(-)-(E)-BETA-CARYOPHYLLENE SYNTHASE	coord: 1..181 score: 9.3

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cla005653	ClCG10G002800	Watermelon (Charleston Gray)	wcgwmB053

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cla005653	Cucumber (Gy14) v2	cgybwmB184
Cla005653	Cucumber (Gy14) v2	cgybwmB348
Cla005653	Melon (DHL92) v3.6.1	medwmB004
Cla005653	Melon (DHL92) v3.6.1	medwmB433
Cla005653	Silver-seed gourd	carwmB0526
Cla005653	Cucumber (Chinese Long) v3	cucwmB205
Cla005653	Cucumber (Chinese Long) v3	cucwmB398
Cla005653	Watermelon (97103) v2	wmwmbB050
Cla005653	Watermelon (97103) v2	wmwmbB053
Cla005653	Wax gourd	wgowmB012
Cla005653	Wax gourd	wgowmB057
Cla005653	Watermelon (97103) v1	wmwmB049
Cla005653	Cucumber (Gy14) v1	cgywmB547
Cla005653	Cucumber (Gy14) v1	cgywmB585
Cla005653	Cucurbita maxima (Rimu)	cmawmB254
Cla005653	Cucurbita maxima (Rimu)	cmawmB295
Cla005653	Cucurbita maxima (Rimu)	cmawmB553
Cla005653	Cucurbita maxima (Rimu)	cmawmB790
Cla005653	Cucurbita moschata (Rifu)	cmowmB245
Cla005653	Cucurbita moschata (Rifu)	cmowmB288
Cla005653	Cucurbita moschata (Rifu)	cmowmB545
Cla005653	Cucurbita moschata (Rifu)	cmowmB784
Cla005653	Wild cucumber (PI 183967)	cpiwmB195
Cla005653	Wild cucumber (PI 183967)	cpiwmB381
Cla005653	Melon (DHL92) v3.5.1	mewmB452
Cla005653	Watermelon (Charleston Gray)	wcgwmB119
Cla005653	Cucumber (Chinese Long) v2	cuwmB195
Cla005653	Cucumber (Chinese Long) v2	cuwmB375
Cla005653	Cucurbita pepo (Zucchini)	cpewmB205
Cla005653	Cucurbita pepo (Zucchini)	cpewmB828
Cla005653	Bottle gourd (USVL1VR-Ls)	lsiwmB008
Cla005653	Bottle gourd (USVL1VR-Ls)	lsiwmB428